Nishioka, Tatsuya; Oda, Yoshifumi; Seino, Yutaka; Yamamoto, Taizo; Inagaki, Nobuya; Yano, Hideki; Imura, Hiroo; Shigemoto, RyuichiISTA ; Kikuchi, Haruhiko
In the present study, we have investigated the expression of both the erythrocyte-type (GLUT1) and the brain-type (GLUT3) glucose transporter isoforms in primary human brain tumors. In situ hybridization made it possible to localize and semiquantify both GLUT1 and GLUT3 mRNAs of individual cells in all 18 samples examined. More signals for GLUT3 mRNA than for GLUT1 mRNA were found over astrocytoma cells, while the reverse was the case in all 6 meningiomas. In astrocytomas, for both mRNAs, the density of silver grains over tumor cells was well correlated with the malignancy of the cells. This correlation was, as was also confirmed by Northern blot analysis, more marked with GLUT3 mRNA than with GLUT1 mRNA. In 2 of 5 anaplastic astrocytomas and in all 3 glioblastomas, numerous tumor cells with large amounts of both mRNAs tended to surround the perivascular regions. 'Tumor vessels' with endothelial proliferation, an almost pathognomonic feature of glioblastomas, expressed much GLUT3 mRNA but no significant GLUT1 mRNA, while a single- or a few-layered capillary endothelium expressed much GLUT1 mRNA. The distribution of both mRNAs was in good accordance with that of both proteins. Our results suggest that the expression of both glucose transporter isoforms may contribute to the maintenance of human brain tumors and that the expression of the GLUT3 isoform may be closely related to the malignant change of astrocytomas and particularly related to the aberrant neovascularization which accompanies glioblastomas.
We wish to acknowledge generous donations of human samples by the following neurosurgeons: Drs. Taro Fukumitsu. Akinori Kondo, Toyoshiro Yamamoto, Juji Takeuchi, Junya Hanakita, Syunichi Yoneda, and Michio Nishikawa. We are very grateful to Dr. G. I. Bell (The University of Chicago) for providing the cDNA clones of GLUTI and GLUT3. We thank Drs. Yoshifumi Yokota, Yuichiro Yamada. and Manabu Fukumoto for their helpful advice. We also thank Yoshinobu Toda and Hiroko Sato for their expert technical assistance. Supported in part by Grants in Aids for Basic Research on Radiation Therapy (03151034) and Special Project Research on Cancer Bio-Science from the Ministry of Education, Science, and Culture of Japan, by Takeda Medical Foundation, and by Monbusho International Scientific Research: Joint Research.
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Nishioka T, Oda Y, Seino Y, et al. Distribution of the glucose transporters in human brain tumors. Cancer Research. 1992;52(14):3972-3979.
Nishioka, T., Oda, Y., Seino, Y., Yamamoto, T., Inagaki, N., Yano, H., … Kikuchi, H. (1992). Distribution of the glucose transporters in human brain tumors. Cancer Research. American Association for Cancer Research.
Nishioka, Tatsuya, Yoshifumi Oda, Yutaka Seino, Taizo Yamamoto, Nobuya Inagaki, Hideki Yano, Hiroo Imura, Ryuichi Shigemoto, and Haruhiko Kikuchi. “Distribution of the Glucose Transporters in Human Brain Tumors.” Cancer Research. American Association for Cancer Research, 1992.
T. Nishioka et al., “Distribution of the glucose transporters in human brain tumors,” Cancer Research, vol. 52, no. 14. American Association for Cancer Research, pp. 3972–3979, 1992.
Nishioka T, Oda Y, Seino Y, Yamamoto T, Inagaki N, Yano H, Imura H, Shigemoto R, Kikuchi H. 1992. Distribution of the glucose transporters in human brain tumors. Cancer Research. 52(14), 3972–3979.
Nishioka, Tatsuya, et al. “Distribution of the Glucose Transporters in Human Brain Tumors.” Cancer Research, vol. 52, no. 14, American Association for Cancer Research, 1992, pp. 3972–79.
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