Molecular characterization of a functional cDNA for rat substance P receptor

Y. Yokota, Y. Sasai, K. Tanaka, T. Fujiwara, K. Tsuchida, R. Shigemoto, A. Kakizuka, H. Ohkubo, S. Nakanishi, Journal of Biological Chemistry 264 (1989) 17649–17652.

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Abstract
This paper describes the amino acid sequence of the rat substance P receptor and its comparison with that of the rat substance K receptor on the basis of molecular cloning and sequence analysis. From a rat brain cDNA library constructed with an RNA expression vector, we identified a cDNA mixture containing a functional substance P receptor cDNA by examining electrophysiologically a receptor expression following injection of the mRNAs synthesized in vitro into Xenopus oocytes. A receptor cDNA clone was then isolated by cross-hybridization with the bovine substance K receptor DNA. The clone was confirmed by selective binding of substance P to the cloned receptor expressed in mammalian COS cells. The deduced amino acid sequence (407 amino acid residues) possesses seven putative membrane spanning domains and shows a sequence similarity to the members of G-protein-coupled receptors. The rat substance P and substance K receptor are very similar in both size and amino acid sequences, particularly in the putative transmembrane similarity is in marked contrast to the sequence divergence in the amino- and carboxyl-terminal regions and the third cytoplasmic loop. The observed sequence similarytity and divergence would thus contribute to the expression of similar but pharmacological regions and the first and second cytoplasmic loops. This distinguishable activities of the two tachykinin receptors.
Publishing Year
Date Published
1989-10-25
Journal Title
Journal of Biological Chemistry
Volume
264
Issue
30
Page
17649 - 17652
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Yokota Y, Sasai Y, Tanaka K, et al. Molecular characterization of a functional cDNA for rat substance P receptor. Journal of Biological Chemistry. 1989;264(30):17649-17652.
Yokota, Y., Sasai, Y., Tanaka, K., Fujiwara, T., Tsuchida, K., Shigemoto, R., … Nakanishi, S. (1989). Molecular characterization of a functional cDNA for rat substance P receptor. Journal of Biological Chemistry, 264(30), 17649–17652.
Yokota, Yoshifumi, Yoshiki Sasai, Kohichi Tanaka, Tsutomu Fujiwara, Kunihiro Tsuchida, Ryuichi Shigemoto, Akira Kakizuka, Hiroaki Ohkubo, and Shigetada Nakanishi. “Molecular Characterization of a Functional CDNA for Rat Substance P Receptor.” Journal of Biological Chemistry 264, no. 30 (1989): 17649–52.
Y. Yokota et al., “Molecular characterization of a functional cDNA for rat substance P receptor,” Journal of Biological Chemistry, vol. 264, no. 30, pp. 17649–17652, 1989.
Yokota Y, Sasai Y, Tanaka K, Fujiwara T, Tsuchida K, Shigemoto R, Kakizuka A, Ohkubo H, Nakanishi S. 1989. Molecular characterization of a functional cDNA for rat substance P receptor. Journal of Biological Chemistry. 264(30), 17649–17652.
Yokota, Yoshifumi, et al. “Molecular Characterization of a Functional CDNA for Rat Substance P Receptor.” Journal of Biological Chemistry, vol. 264, no. 30, American Society for Biochemistry and Molecular Biology, 1989, pp. 17649–52.

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