NMDA receptors in hippocampal GABAergic synapses and their role in nitric oxide signaling

E. Szabadits, C. Cserép, A. Szonyi, Y. Fukazawa, R. Shigemoto, M. Watanabe, S. Itohara, T. Freund, G. Nyíri, Journal of Neuroscience 31 (2011) 5893–5904.

Download
No fulltext has been uploaded. References only!

Journal Article | Published
Author
Szabadits, Eszter; Cserép, Csaba; Szonyi, András; Fukazawa, Yugo; Shigemoto, RyuichiIST Austria ; Watanabe, Masahiko; Itohara, Shigeyoshi; Freund, Tamás F; Nyíri, Gábor
Abstract
GABAergic inhibition plays a central role in the control of pyramidal cell ensemble activities; thus, any signaling mechanism that regulates inhibition is able to fine-tune network patterns. Here, we provide evidence that the retrograde nitric oxide (NO)- cGMP cascade triggered by NMDA receptor (NMDAR) activation plays a role in the control of hippocampal GABAergic transmission in mice. GABAergic synapses express neuronal nitric oxide synthase (nNOS) postsynaptically and NO receptors (NO-sensitive guanylyl cyclase) in the presynaptic terminals. We hypothesized that-similar to glutamatergic synapses-the Ca 2+ transients required to activate nNOS were provided by NMDA receptor activation. Indeed, administration of 5 μm NMDA induced a robust nNOS-dependent cGMP production in GABAergic terminals, selectively in the CA1 and CA3c areas. Furthermore, using preembedding, postembedding, and SDS-digested freeze-fracture replica immunogold labeling, we provided quantitative immunocytochemical evidence that NMDAR subunits GluN1, GluN2A, and GluN2B were present in most somatic GABAergic synapses postsynaptically. These data indicate that NMDARs can modulate hippocampal GABAergic inhibition via NO- cGMP signaling in an activity-dependent manner and that this effect is subregion specific in the mouse hippocampus.
Publishing Year
Date Published
2011-04-20
Journal Title
Journal of Neuroscience
Volume
31
Issue
16
Page
5893 - 5904
IST-REx-ID

Cite this

Szabadits E, Cserép C, Szonyi A, et al. NMDA receptors in hippocampal GABAergic synapses and their role in nitric oxide signaling. Journal of Neuroscience. 2011;31(16):5893-5904. doi:10.1523/JNEUROSCI.5938-10.2011
Szabadits, E., Cserép, C., Szonyi, A., Fukazawa, Y., Shigemoto, R., Watanabe, M., … Nyíri, G. (2011). NMDA receptors in hippocampal GABAergic synapses and their role in nitric oxide signaling. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.5938-10.2011
Szabadits, Eszter, Csaba Cserép, András Szonyi, Yugo Fukazawa, Ryuichi Shigemoto, Masahiko Watanabe, Shigeyoshi Itohara, Tamás Freund, and Gábor Nyíri. “NMDA Receptors in Hippocampal GABAergic Synapses and Their Role in Nitric Oxide Signaling.” Journal of Neuroscience. Society for Neuroscience, 2011. https://doi.org/10.1523/JNEUROSCI.5938-10.2011.
E. Szabadits et al., “NMDA receptors in hippocampal GABAergic synapses and their role in nitric oxide signaling,” Journal of Neuroscience, vol. 31, no. 16. Society for Neuroscience, pp. 5893–5904, 2011.
Szabadits E, Cserép C, Szonyi A, Fukazawa Y, Shigemoto R, Watanabe M, Itohara S, Freund T, Nyíri G. 2011. NMDA receptors in hippocampal GABAergic synapses and their role in nitric oxide signaling. Journal of Neuroscience. 31(16), 5893–5904.
Szabadits, Eszter, et al. “NMDA Receptors in Hippocampal GABAergic Synapses and Their Role in Nitric Oxide Signaling.” Journal of Neuroscience, vol. 31, no. 16, Society for Neuroscience, 2011, pp. 5893–904, doi:10.1523/JNEUROSCI.5938-10.2011.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar