--- _id: '2508' abstract: - lang: eng text: 'The activity patterns of subthalamic nucleus (STN) neurons are intimately linked to motor function and dysfunction and arise through the complex interaction of intrinsic properties and inhibitory and excitatory synaptic inputs. In many neurons, hyperpolarization-activated cyclic nucleotide-gated (HCN) channels play key roles in intrinsic excitability and synaptic integration both under normal conditions and in disease states. However, in STN neurons, which strongly express HCN channels, their roles remain relatively obscure. To address this deficit, complementary molecular and cellular electrophysiological, imaging, and computational approaches were applied to the rat STN. Molecular profiling demonstrated that individual STN neurons express mRNA encoding several HCN subunits, with HCN2 and 3 being the most abundant. Light and electron microscopic analysis showed that HCN2 subunits are strongly expressed and distributed throughout the somatodendritic plasma membrane. Voltage-, current-, and dynamic-clamp analysis, two-photon Ca 2+ imaging, and computational modeling revealed that HCN channels are activated by GABA A receptor-mediated inputs and thus limit synaptic hyperpolarization and deinactivation of low-voltage-activated Ca 2+ channels. Although HCN channels also limited the temporal summation of EPSPs, generated through two-photon uncaging of glutamate, this action was largely shunted by GABAergic inhibition that was necessary for HCN channel activation. Together the data demonstrate that HCN channels in STN neurons selectively counteract GABA A receptor-mediated inhibition arising from the globus pallidus and thus promote single-spike activity rather than rebound burst firing. ' author: - first_name: Jeremy full_name: Atherton, Jeremy F last_name: Atherton - first_name: Katsunori full_name: Kitano, Katsunori last_name: Kitano - first_name: Jérôme full_name: Baufreton, Jérôme last_name: Baufreton - first_name: Kai full_name: Fan, Kai last_name: Fan - first_name: David full_name: Wokosin, David L last_name: Wokosin - first_name: Tatiana full_name: Tkatch, Tatiana last_name: Tkatch - first_name: Ryuichi full_name: Ryuichi Shigemoto id: 499F3ABC-F248-11E8-B48F-1D18A9856A87 last_name: Shigemoto orcid: 0000-0001-8761-9444 - first_name: James full_name: Surmeier, James D last_name: Surmeier - first_name: Mark full_name: Bevan, Mark D last_name: Bevan citation: ama: Atherton J, Kitano K, Baufreton J, et al. Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus. Journal of Neuroscience. 2010;30(47):16025-16040. doi:10.1523/JNEUROSCI.3898-10.2010 apa: Atherton, J., Kitano, K., Baufreton, J., Fan, K., Wokosin, D., Tkatch, T., … Bevan, M. (2010). Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.3898-10.2010 chicago: Atherton, Jeremy, Katsunori Kitano, Jérôme Baufreton, Kai Fan, David Wokosin, Tatiana Tkatch, Ryuichi Shigemoto, James Surmeier, and Mark Bevan. “Selective Participation of Somatodendritic HCN Channels in Inhibitory but Not Excitatory Synaptic Integration in Neurons of the Subthalamic Nucleus.” Journal of Neuroscience. Society for Neuroscience, 2010. https://doi.org/10.1523/JNEUROSCI.3898-10.2010. ieee: J. Atherton et al., “Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus,” Journal of Neuroscience, vol. 30, no. 47. Society for Neuroscience, pp. 16025–16040, 2010. ista: Atherton J, Kitano K, Baufreton J, Fan K, Wokosin D, Tkatch T, Shigemoto R, Surmeier J, Bevan M. 2010. Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus. Journal of Neuroscience. 30(47), 16025–16040. mla: Atherton, Jeremy, et al. “Selective Participation of Somatodendritic HCN Channels in Inhibitory but Not Excitatory Synaptic Integration in Neurons of the Subthalamic Nucleus.” Journal of Neuroscience, vol. 30, no. 47, Society for Neuroscience, 2010, pp. 16025–40, doi:10.1523/JNEUROSCI.3898-10.2010. short: J. Atherton, K. Kitano, J. Baufreton, K. Fan, D. Wokosin, T. Tkatch, R. Shigemoto, J. Surmeier, M. Bevan, Journal of Neuroscience 30 (2010) 16025–16040. date_created: 2018-12-11T11:58:05Z date_published: 2010-11-24T00:00:00Z date_updated: 2021-01-12T06:57:54Z day: '24' doi: 10.1523/JNEUROSCI.3898-10.2010 extern: 1 intvolume: ' 30' issue: '47' month: '11' page: 16025 - 16040 publication: Journal of Neuroscience publication_status: published publisher: Society for Neuroscience publist_id: '4393' quality_controlled: 0 status: public title: Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus type: journal_article volume: 30 year: '2010' ...