--- res: bibo_abstract: - Subepithelial fibroblasts of the intestinal villi, which form a contractile cellular network beneath the epithelium, are in close contact with epithelial cells, nerve varicosities, capillaries, smooth muscles and immune cells, and secrete extracellular matrix molecules, growth factors and cytokines, etc. Cultured subepithelial fibroblasts of the rat duodenal villi display various receptors such as endothelins, ATP, substance-P and bradykinin, and release ATP in response to mechanical stimulation. In this study, the presence of functional NK1 receptors (NK1R) was pharmacologically confirmed in primary culture by Ca 2+ measurement, and the effects of substance-P were measured in an acute preparation of epithelium-free duodenal villi from 2- to 3-week-old rats using a two-photon laser microscope. Substance-P elicited an increase in the intracellular Ca 2+ concentration and contraction of the subepithelial fibroblasts in culture and the isolated villi. The localization of NK1R and substance-P in the villi was examined by light and electron microscopic immunohistochemistry. NK1R-like immunoreactivity was intensely localized on the plasma membrane of villous subepithelial fibroblasts in 10-day- to 4-week-old rats and mice and was decreased or absent in adulthood. The pericryptal fibroblasts of the small and large intestine were NK1R immuno-negative. These villous subepithelial fibroblasts form synapse-like structures with both substance-P-immunopositive and -immunonegative nerve varicosities. Here, we propose that the mutual interaction between villous subepithelial fibroblasts and afferent neurons via substance-P and ATP plays important roles in the maturation of the structure and function of the small intestine.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Sonoko foaf_name: Furuya, Sonoko foaf_surname: Furuya - foaf_Person: foaf_givenName: Kishio foaf_name: Furuya, Kishio foaf_surname: Furuya - foaf_Person: foaf_givenName: Ryuichi foaf_name: Ryuichi Shigemoto foaf_surname: Shigemoto foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8761-9444 - foaf_Person: foaf_givenName: Masahiro foaf_name: Sokabe, Masahiro foaf_surname: Sokabe bibo_doi: 10.1007/s00441-010-1056-7 bibo_issue: '2' bibo_volume: 342 dct_date: 2010^xs_gYear dct_publisher: Springer@ dct_title: Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat intestinal villi@ ...