Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo

Grinevich V, Kolleker A, Eliava M, Takada N, Takuma H, Fukazawa Y, Shigemoto R, Kuhl D, Waters J, Seeburg P, Osten P. 2009. Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods. 184(1), 25–36.

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Author
Grinevich, Valery V; Kolleker, Alexander; Eliava, Marina I; Takada, Naoki; Takuma, Hiroshi; Fukazawa, Yugo; Shigemoto, RyuichiIST Austria ; Kuhl, Dietmar; Waters, Jack; Seeburg, Peter H; Osten, Pavel
Abstract
The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.
Publishing Year
Date Published
2009-10-30
Journal Title
Journal of Neuroscience Methods
Volume
184
Issue
1
Page
25 - 36
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Grinevich V, Kolleker A, Eliava M, et al. Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods. 2009;184(1):25-36. doi:10.1016/j.jneumeth.2009.07.015
Grinevich, V., Kolleker, A., Eliava, M., Takada, N., Takuma, H., Fukazawa, Y., … Osten, P. (2009). Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods. Elsevier. https://doi.org/10.1016/j.jneumeth.2009.07.015
Grinevich, Valery, Alexander Kolleker, Marina Eliava, Naoki Takada, Hiroshi Takuma, Yugo Fukazawa, Ryuichi Shigemoto, et al. “Fluorescent Arc/Arg3.1 Indicator Mice: A Versatile Tool to Study Brain Activity Changes in Vitro and in Vivo.” Journal of Neuroscience Methods. Elsevier, 2009. https://doi.org/10.1016/j.jneumeth.2009.07.015.
V. Grinevich et al., “Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo,” Journal of Neuroscience Methods, vol. 184, no. 1. Elsevier, pp. 25–36, 2009.
Grinevich V, Kolleker A, Eliava M, Takada N, Takuma H, Fukazawa Y, Shigemoto R, Kuhl D, Waters J, Seeburg P, Osten P. 2009. Fluorescent Arc/Arg3.1 indicator mice: A versatile tool to study brain activity changes in vitro and in vivo. Journal of Neuroscience Methods. 184(1), 25–36.
Grinevich, Valery, et al. “Fluorescent Arc/Arg3.1 Indicator Mice: A Versatile Tool to Study Brain Activity Changes in Vitro and in Vivo.” Journal of Neuroscience Methods, vol. 184, no. 1, Elsevier, 2009, pp. 25–36, doi:10.1016/j.jneumeth.2009.07.015.

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