Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses

Tarusawa E, Matsui K, Budisantoso T, Molnár E, Watanabe M, Matsui M, Fukazawa Y, Shigemoto R. 2009. Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses. Journal of Neuroscience. 29(41), 12896–12908.

Download
No fulltext has been uploaded. References only!

Journal Article | Published
Author
Tarusawa, Etsuko; Matsui, Ko; Budisantoso, Timotheus; Molnár, Elek; Watanabe, Masahiko; Matsui, Minoru; Fukazawa, Yugo; Shigemoto, RyuichiIST Austria
Abstract
To examine the intrasynaptic arrangement of postsynaptic receptors in relation to the functional role of the synapse,we quantitatively analyzed the two-dimensional distribution of AMPA and NMDA receptors (AMPARs and NMDARs, respectively) using SDS-digested freeze-fracture replica labeling (SDS-FRL) and assessed the implication of distribution differences on the postsynaptic responses by simulation. In the dorsal lateral geniculate nucleus, corticogeniculate (CG) synapses were twice as large as retinogeniculate (RG) synapses but expressed similar numbers of AMPARs. Two-dimensional views of replicas revealed that AMPARs form microclusters in both synapses to a similar extent, resulting in larger AMPAR-lacking areas in the CG synapses. Despite the broad difference in the AMPAR distribution within a synapse, our simulations based on the actual receptor distributions suggested that the AMPAR quantal response at individual RG synapses is only slightly larger in amplitude, less variable, and faster in kinetics than that at CG synapses having a similar number of the receptors. NMDARs at the CG synapses were expressed twice as many as those in the RG synapses. Electrophysiological recordings confirmed a larger contribution of NMDAR relative to AMPAR-mediated responses in CG synapses. We conclude that synapse size and the density and distribution of receptors have minor influences on quantal responses and that the number of receptors acts as a predominant postsynaptic determinant of the synaptic strength mediated by both the AMPARs and NMDARs.
Publishing Year
Date Published
2009-10-14
Journal Title
Journal of Neuroscience
Volume
29
Issue
41
Page
12896 - 12908
IST-REx-ID

Cite this

Tarusawa E, Matsui K, Budisantoso T, et al. Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses. Journal of Neuroscience. 2009;29(41):12896-12908. doi:10.1523/JNEUROSCI.6160-08.2009
Tarusawa, E., Matsui, K., Budisantoso, T., Molnár, E., Watanabe, M., Matsui, M., … Shigemoto, R. (2009). Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.6160-08.2009
Tarusawa, Etsuko, Ko Matsui, Timotheus Budisantoso, Elek Molnár, Masahiko Watanabe, Minoru Matsui, Yugo Fukazawa, and Ryuichi Shigemoto. “Input-Specific Intrasynaptic Arrangements of Ionotropic Glutamate Receptors and Their Impact on Postsynaptic Responses.” Journal of Neuroscience. Society for Neuroscience, 2009. https://doi.org/10.1523/JNEUROSCI.6160-08.2009.
E. Tarusawa et al., “Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses,” Journal of Neuroscience, vol. 29, no. 41. Society for Neuroscience, pp. 12896–12908, 2009.
Tarusawa E, Matsui K, Budisantoso T, Molnár E, Watanabe M, Matsui M, Fukazawa Y, Shigemoto R. 2009. Input-specific intrasynaptic arrangements of ionotropic glutamate receptors and their impact on postsynaptic responses. Journal of Neuroscience. 29(41), 12896–12908.
Tarusawa, Etsuko, et al. “Input-Specific Intrasynaptic Arrangements of Ionotropic Glutamate Receptors and Their Impact on Postsynaptic Responses.” Journal of Neuroscience, vol. 29, no. 41, Society for Neuroscience, 2009, pp. 12896–908, doi:10.1523/JNEUROSCI.6160-08.2009.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar