Sugimoto, Yukihiko; Shigemoto, RyuichiISTA ; Namba, Tsunehisa; Negishi, Manabu; Mizuno, Noboru; Narumiya, Shuh; Ichikawa, Atsushi
Distribution of the messenger RNA for the prostaglandin E receptor subtype EP3 was investigated by in situ hybridization in the nervous system of the mouse. The hybridization signals for EP3 were widely distributed in the brain and sensory ganglia and specifically localized to neurons. In the dorsal root and trigeminal ganglia, about half of the neurons were labeled intensely. In the brain, intensely labeled neurons were found in Ammon's horn, the preoptic nuclei, lateral hypothalamic area, dorsomedial hypothalamic nucleus, lateral mammillary nucleus, entopeduncular nucleus, substantia nigra pars compacta, locus coeruleus and raphe nuclei. Moderately labeled neurons were seen in the mitral cell layer of the main olfactory bulb, layer V of the entorhinal and parasubicular cortices, layers V and VI of the cerebral neocortex, nuclei of the diagonal band, magnocellular preoptic nucleus, globus pallidus and lateral parabrachial nucleus. In the thalamus, moderately labeled neurons were distributed in the anterior, ventromedial, laterodorsal, paraventricular and central medial nuclei. Based on these distributions, we suggest that EP3 not only mediates prostaglandin E2 signals evoked by blood-borne cytokines in the areas poor in the blood-brain barrier, but also responds to those formed intrinsically within the brain to modulate various neuronal activities. Possible EP3 actions are discussed in relation to the reported neuronal activities of prostaglandin E2 in the brain.
This work was supported in part by Grants-in-aid for Scientific Research 05404020, 04255103. 05771975, 05671816 and 05454568 from the Ministry of Education, Science and Culture of Japan and by grants from the Mitsubishi Foundation and the Takeda Science Foundation. We are grateful to Mr Akira Uesugi for photographic help. We also thank Drs Chihiro Akazawa, Hitoshi Ohishi and Masabumi Minami for helpful discussions.
919 - 928
Sugimoto Y, Shigemoto R, Namba T, et al. Distribution of the messenger rna for the prostaglandin e receptor subtype ep3 in the mouse nervous system. Neuroscience. 1994;62(3):919-928. doi:10.1016/0306-4522(94)90483-9
Sugimoto, Y., Shigemoto, R., Namba, T., Negishi, M., Mizuno, N., Narumiya, S., & Ichikawa, A. (1994). Distribution of the messenger rna for the prostaglandin e receptor subtype ep3 in the mouse nervous system. Neuroscience. Elsevier. https://doi.org/10.1016/0306-4522(94)90483-9
Sugimoto, Yukihiko, Ryuichi Shigemoto, Tsunehisa Namba, Manabu Negishi, Noboru Mizuno, Shuh Narumiya, and Atsushi Ichikawa. “Distribution of the Messenger Rna for the Prostaglandin e Receptor Subtype Ep3 in the Mouse Nervous System.” Neuroscience. Elsevier, 1994. https://doi.org/10.1016/0306-4522(94)90483-9.
Y. Sugimoto et al., “Distribution of the messenger rna for the prostaglandin e receptor subtype ep3 in the mouse nervous system,” Neuroscience, vol. 62, no. 3. Elsevier, pp. 919–928, 1994.
Sugimoto Y, Shigemoto R, Namba T, Negishi M, Mizuno N, Narumiya S, Ichikawa A. 1994. Distribution of the messenger rna for the prostaglandin e receptor subtype ep3 in the mouse nervous system. Neuroscience. 62(3), 919–928.
Sugimoto, Yukihiko, et al. “Distribution of the Messenger Rna for the Prostaglandin e Receptor Subtype Ep3 in the Mouse Nervous System.” Neuroscience, vol. 62, no. 3, Elsevier, 1994, pp. 919–28, doi:10.1016/0306-4522(94)90483-9.
Link(s) to Main File(s)