Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex

T. Kaneko, R. Shigemoto, S. Nakanishi, N. Mizuno, Neuroscience 60 (1994) 199–211.

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Abstract
Substance P receptor-expressing neurons in the rat cerebral neocortex were examined by single- and double-immunolabeling methods with an affinity-purified specific antibody to substance P receptor. Substance P receptor immunoreactivity was observed exclusively in non-pyramidal neurons. About a quarter of these substance P receptor-positive neocortical neurons showed intense immunoreactivity, and the other three quarters displayed weak substance P receptor immunoreactivity. The neurons showing intense substance P receptor immunoreactivity were large multipolar cells with a few long aspiny or sparsely-spiny dendrites, and were scattered throughout the neocortical layers except for layer I, and also in the underlying white matter. The weakly immunoreactive neurons were medium-sized multipolar cells with oval to round somata and aspiny varicose dendrites, and were distributed in all cortical layers with a bias to layers II-III and the superficial part of layer V. The double-immunofluorescence study revealed that almost all substance P receptor-positive neurons were immunoreactive for GABA, but negative for glutaminase. Substance P receptor immunoreactivity in GABAergic neocortical neurons were further examined by the double-immunofluorescence method with antibodies to markers for subgroups of GABAergic neurons. Somatostatin immunoreactivity was found in 89% of neurons with intense substance P receptor immunoreactivity, and in 1.5% of neurons with weak substance P receptor immunoreactivity. Neuropeptide Y immunoreactivity was also observed in 92% of neurons with intense immunoreactivity for substance P receptor, and in 1.6% of neurons with weak immunoreactivity for substance P receptor. In contrast, parvalbumin immunoreactivity was seen in 1.3% of neurons with intense substance P receptor immunoreactivity, and in 59% of weak substance P receptor immunoreactivity. Calbindin D28k immunoreactivity was found in 12 and 19% of neurons, respectively, with weak and intense immunoreactivities for substance P receptor. Virtually no cells showing substance P receptor immunoreactivity displayed immunoreactivity for vasoactive intestinal polypeptide or choline acetyltransferase. These results indicate that the neocortical neurons expressing substance P receptor constitute a subpopulation of GABAergic non-pyramidal cells, and are segregated into neurons with intense immunoreactivity and those with weak immunoreactivity for substance P receptor; the vast majority of neurons with intense substance P receptor immunoreactivity contain somatostatin and neuropeptide Y, and the majority of neurons with weak substance P receptor immunoreactivity have parvalbumin.
Publishing Year
Date Published
1994-05-01
Journal Title
Neuroscience
Volume
60
Issue
1
Page
199 - 211
IST-REx-ID

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Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience. 1994;60(1):199-211. doi:10.1016/0306-4522(94)90215-1
Kaneko, T., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1994). Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience, 60(1), 199–211. https://doi.org/10.1016/0306-4522(94)90215-1
Kaneko, Takeshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno. “Morphological and Chemical Characteristics of Substance P Receptor Immunoreactive Neurons in the Rat Neocortex.” Neuroscience 60, no. 1 (1994): 199–211. https://doi.org/10.1016/0306-4522(94)90215-1.
T. Kaneko, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex,” Neuroscience, vol. 60, no. 1, pp. 199–211, 1994.
Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1994. Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience. 60(1), 199–211.
Kaneko, Takeshi, et al. “Morphological and Chemical Characteristics of Substance P Receptor Immunoreactive Neurons in the Rat Neocortex.” Neuroscience, vol. 60, no. 1, Elsevier, 1994, pp. 199–211, doi:10.1016/0306-4522(94)90215-1.

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