Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex

T. Kaneko, R. Shigemoto, S. Nakanishi, N. Mizuno, Neuroscience 60 (1994) 199–211.

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Author
Kaneko, Takeshi; Shigemoto, RyuichiIST Austria ; Nakanishi, Shigetada; Mizuno, Noboru
Abstract
Substance P receptor-expressing neurons in the rat cerebral neocortex were examined by single- and double-immunolabeling methods with an affinity-purified specific antibody to substance P receptor. Substance P receptor immunoreactivity was observed exclusively in non-pyramidal neurons. About a quarter of these substance P receptor-positive neocortical neurons showed intense immunoreactivity, and the other three quarters displayed weak substance P receptor immunoreactivity. The neurons showing intense substance P receptor immunoreactivity were large multipolar cells with a few long aspiny or sparsely-spiny dendrites, and were scattered throughout the neocortical layers except for layer I, and also in the underlying white matter. The weakly immunoreactive neurons were medium-sized multipolar cells with oval to round somata and aspiny varicose dendrites, and were distributed in all cortical layers with a bias to layers II-III and the superficial part of layer V. The double-immunofluorescence study revealed that almost all substance P receptor-positive neurons were immunoreactive for GABA, but negative for glutaminase. Substance P receptor immunoreactivity in GABAergic neocortical neurons were further examined by the double-immunofluorescence method with antibodies to markers for subgroups of GABAergic neurons. Somatostatin immunoreactivity was found in 89% of neurons with intense substance P receptor immunoreactivity, and in 1.5% of neurons with weak substance P receptor immunoreactivity. Neuropeptide Y immunoreactivity was also observed in 92% of neurons with intense immunoreactivity for substance P receptor, and in 1.6% of neurons with weak immunoreactivity for substance P receptor. In contrast, parvalbumin immunoreactivity was seen in 1.3% of neurons with intense substance P receptor immunoreactivity, and in 59% of weak substance P receptor immunoreactivity. Calbindin D28k immunoreactivity was found in 12 and 19% of neurons, respectively, with weak and intense immunoreactivities for substance P receptor. Virtually no cells showing substance P receptor immunoreactivity displayed immunoreactivity for vasoactive intestinal polypeptide or choline acetyltransferase. These results indicate that the neocortical neurons expressing substance P receptor constitute a subpopulation of GABAergic non-pyramidal cells, and are segregated into neurons with intense immunoreactivity and those with weak immunoreactivity for substance P receptor; the vast majority of neurons with intense substance P receptor immunoreactivity contain somatostatin and neuropeptide Y, and the majority of neurons with weak substance P receptor immunoreactivity have parvalbumin.
Publishing Year
Date Published
1994-05-01
Journal Title
Neuroscience
Volume
60
Issue
1
Page
199 - 211
IST-REx-ID

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Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience. 1994;60(1):199-211. doi:10.1016/0306-4522(94)90215-1
Kaneko, T., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1994). Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience. Elsevier. https://doi.org/10.1016/0306-4522(94)90215-1
Kaneko, Takeshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno. “Morphological and Chemical Characteristics of Substance P Receptor Immunoreactive Neurons in the Rat Neocortex.” Neuroscience. Elsevier, 1994. https://doi.org/10.1016/0306-4522(94)90215-1.
T. Kaneko, R. Shigemoto, S. Nakanishi, and N. Mizuno, “Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex,” Neuroscience, vol. 60, no. 1. Elsevier, pp. 199–211, 1994.
Kaneko T, Shigemoto R, Nakanishi S, Mizuno N. 1994. Morphological and chemical characteristics of substance P receptor immunoreactive neurons in the rat neocortex. Neuroscience. 60(1), 199–211.
Kaneko, Takeshi, et al. “Morphological and Chemical Characteristics of Substance P Receptor Immunoreactive Neurons in the Rat Neocortex.” Neuroscience, vol. 60, no. 1, Elsevier, 1994, pp. 199–211, doi:10.1016/0306-4522(94)90215-1.

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