Shigemoto, RyuichiISTA ; Yokota, Yoshifumi; Tsuchida, Kunihiro; Nakanishi, Shigetada
Functional cDNA clones for rat neuromedin K receptor were isolated from a rat brain cDNA library by cross-hybridization with the bovine substance K recepor cDNA. Injection of the mRNA synthesized in vitro from the cloned cDNA into Xenopus oocytes elicited electrophysiological responses to tachykinins, with the most potent sensitivity being to neuromedin K. Ligand-binding displacement in membranes of mammalian COS cells transfected with the cDNA indicated the rank order of affinity of the receptor to tachykinins; neuromedin K > substance K > substance P. The hybridization analysis showed that the neuromedin K receptor mRNA is expressed in both the brain and the peripheral tissues at different levels. The rat neuromedin K receptor consists of 452 amino acid residues and belongs to the family of G protein-coupled receptors, which are thought to have seven transmembrane domains. The sequence comparison of the rat neuromedin K, substance P, and substance K receptors revealed that these receptors are highly conserved in the seven transmembrane domains and the cytoplasmic sides of the receptors. They also show some structural characteristics, including the common presence of histidine residues in transmembrane segments V and VI and the difference in the numbers and distributions of serine and threonine residues as possible phosphorylation sites in the cytoplasmic regions. This paper thus presents the first comprehensive analysis of the molecular nature of the multiple peptide receptors that exhibit similar but pharmacologically distinguishable activities.
Journal of Biological Chemistry
This work was supported in part by research grants from the Ministry Education, Science and Culture of Japan; the Institute of Physical and Chemical Research; and the Science and Technology Agency of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC. Section 1734 solely to indicate this fact.
623 - 628
Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S. Cloning and expression of a rat neuromedin K receptor cDNA. Journal of Biological Chemistry. 1990;265(2):623-628. doi:10.1016/s0021-9258(19)40095-1
Shigemoto, R., Yokota, Y., Tsuchida, K., & Nakanishi, S. (1990). Cloning and expression of a rat neuromedin K receptor cDNA. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1016/s0021-9258(19)40095-1
Shigemoto, Ryuichi, Yoshifumi Yokota, Kunihiro Tsuchida, and Shigetada Nakanishi. “Cloning and Expression of a Rat Neuromedin K Receptor CDNA.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 1990. https://doi.org/10.1016/s0021-9258(19)40095-1 .
R. Shigemoto, Y. Yokota, K. Tsuchida, and S. Nakanishi, “Cloning and expression of a rat neuromedin K receptor cDNA,” Journal of Biological Chemistry, vol. 265, no. 2. American Society for Biochemistry and Molecular Biology, pp. 623–628, 1990.
Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S. 1990. Cloning and expression of a rat neuromedin K receptor cDNA. Journal of Biological Chemistry. 265(2), 623–628.
Shigemoto, Ryuichi, et al. “Cloning and Expression of a Rat Neuromedin K Receptor CDNA.” Journal of Biological Chemistry, vol. 265, no. 2, American Society for Biochemistry and Molecular Biology, 1990, pp. 623–28, doi:10.1016/s0021-9258(19)40095-1 .
All files available under the following license(s):
This Item is protected by copyright and/or related rights. [...]
Link(s) to Main File(s)