Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis

G. Novarino, S. Weinert, G. Rickheit, T. Jentsch, Science 328 (2010) 1398–1401.

Download
No fulltext has been uploaded. References only!

Journal Article | Published
Author
Novarino, GaiaIST Austria ; Weinert, Stefanie; Rickheit, Gesa; Jentsch, Thomas J
Abstract
Loss of the endosomal anion transport protein ClC-5 impairs renal endocytosis and underlies human Dent's disease. ClC-5 is thought to promote endocytosis by facilitating endosomal acidification through the neutralization of proton pump currents. However, ClC-5 is a 2 chloride (Cl-)/proton (H+) exchanger rather than a Cl- channel. We generated mice that carry the uncoupling E211A (unc) mutation that converts CLC-5 into a pure CL- conductor. Adenosine triphosphate (ATP)-dependent acidification of renal endosomes was reduced in mice in which ClC-5 was knocked out, but normal in Clcn5unc mice. However, their proximal tubular endocytosis was also impaired. Thus, endosomal chloride concentration, which is raised by CLC-5 in exchange for protons accumulated by the H+-ATPase, may play a role in endocytosis.
Publishing Year
Date Published
2010-06-11
Journal Title
Science
Volume
328
Issue
5984
Page
1398 - 1401
IST-REx-ID

Cite this

Novarino G, Weinert S, Rickheit G, Jentsch T. Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis. Science. 2010;328(5984):1398-1401. doi:10.1126/science.1188070
Novarino, G., Weinert, S., Rickheit, G., & Jentsch, T. (2010). Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis. Science, 328(5984), 1398–1401. https://doi.org/10.1126/science.1188070
Novarino, Gaia, Stefanie Weinert, Gesa Rickheit, and Thomas Jentsch. “Endosomal Chloride-Proton Exchange Rather than Chloride Conductance Is Crucial for Renal Endocytosis.” Science 328, no. 5984 (2010): 1398–1401. https://doi.org/10.1126/science.1188070.
G. Novarino, S. Weinert, G. Rickheit, and T. Jentsch, “Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis,” Science, vol. 328, no. 5984, pp. 1398–1401, 2010.
Novarino G, Weinert S, Rickheit G, Jentsch T. 2010. Endosomal chloride-proton exchange rather than chloride conductance is crucial for renal endocytosis. Science. 328(5984), 1398–1401.
Novarino, Gaia, et al. “Endosomal Chloride-Proton Exchange Rather than Chloride Conductance Is Crucial for Renal Endocytosis.” Science, vol. 328, no. 5984, American Association for the Advancement of Science, 2010, pp. 1398–401, doi:10.1126/science.1188070.

Export

Marked Publications

Open Data IST Research Explorer

Search this title in

Google Scholar