--- _id: '2253' abstract: - lang: eng text: Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation. article_processing_charge: No article_type: original author: - first_name: Aurélien full_name: Bailly, Aurélien last_name: Bailly - first_name: Bangjun full_name: Wang, Bangjun last_name: Wang - first_name: Marta full_name: Zwiewka, Marta last_name: Zwiewka - first_name: Stephan full_name: Pollmann, Stephan last_name: Pollmann - first_name: Daniel full_name: Schenck, Daniel last_name: Schenck - first_name: Hartwig full_name: Lüthen, Hartwig last_name: Lüthen - first_name: Alexander full_name: Schulz, Alexander last_name: Schulz - first_name: Jirí full_name: Friml, Jirí id: 4159519E-F248-11E8-B48F-1D18A9856A87 last_name: Friml orcid: 0000-0002-8302-7596 - first_name: Markus full_name: Geisler, Markus last_name: Geisler citation: ama: Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 2014;77(1):108-118. doi:10.1111/tpj.12369 apa: Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. Wiley-Blackwell. https://doi.org/10.1111/tpj.12369 chicago: Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” Plant Journal. Wiley-Blackwell, 2014. https://doi.org/10.1111/tpj.12369. ieee: A. Bailly et al., “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” Plant Journal, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014. ista: Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118. mla: Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” Plant Journal, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:10.1111/tpj.12369. short: A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118. date_created: 2018-12-11T11:56:35Z date_published: 2014-01-01T00:00:00Z date_updated: 2021-01-12T06:56:18Z day: '01' department: - _id: JiFr doi: 10.1111/tpj.12369 intvolume: ' 77' issue: '1' language: - iso: eng main_file_link: - open_access: '1' url: https://doi.org/10.1111/tpj.12369 month: '01' oa: 1 oa_version: Published Version page: 108 - 118 project: - _id: 256BDAB0-B435-11E9-9278-68D0E5697425 name: Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur publication: Plant Journal publication_identifier: issn: - '09607412' publication_status: published publisher: Wiley-Blackwell publist_id: '4694' quality_controlled: '1' scopus_import: 1 status: public title: Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 77 year: '2014' ...