Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters

S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:, G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.

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Book Chapter | Published | English
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Methods in Molecular Biology
Abstract
Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application.
Publishing Year
Date Published
2014-01-01
Book Title
Plant Chemical Genomics
Volume
1056
Page
255 - 264
ISSN
IST-REx-ID

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Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds. Plant Chemical Genomics. Vol 1056. Methods in Molecular Biology. Springer; 2014:255-264. doi:10.1007/978-1-62703-592-7_23
Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., & Friml, J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In G. Hicks & S. Robert (Eds.), Plant Chemical Genomics (Vol. 1056, pp. 255–264). Springer. https://doi.org/10.1007/978-1-62703-592-7_23
Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and Jirí Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” In Plant Chemical Genomics, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64. Methods in Molecular Biology. Springer, 2014. https://doi.org/10.1007/978-1-62703-592-7_23.
S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml, “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters,” in Plant Chemical Genomics, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014, pp. 255–264.
Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. Plant Chemical Genomics. Methods in Molecular Biology, Methods in Molecular Biology, vol. 1056. 255–264.
Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” Plant Chemical Genomics, edited by Glenn Hicks and Stéphanie Robert, vol. 1056, Springer, 2014, pp. 255–64, doi:10.1007/978-1-62703-592-7_23.

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