---
_id: '2084'
abstract:
- lang: eng
text: Receptor tyrosine kinases (RTKs) are a large family of cell surface receptors
that sense growth factors and hormones and regulate a variety of cell behaviours
in health and disease. Contactless activation of RTKs with spatial and temporal
precision is currently not feasible. Here, we generated RTKs that are insensitive
to endogenous ligands but can be selectively activated by low-intensity blue light.
We screened light-oxygen-voltage (LOV)-sensing domains for their ability to activate
RTKs by light-activated dimerization. Incorporation of LOV domains found in aureochrome
photoreceptors of stramenopiles resulted in robust activation of the fibroblast
growth factor receptor 1 (FGFR1), epidermal growth factor receptor (EGFR) and
rearranged during transfection (RET). In human cancer and endothelial cells, light
induced cellular signalling with spatial and temporal precision. Furthermore,
light faithfully mimicked complex mitogenic and morphogenic cell behaviour induced
by growth factors. RTKs under optical control (Opto-RTKs) provide a powerful optogenetic
approach to actuate cellular signals and manipulate cell behaviour.
acknowledgement: European Union Seventh Framework Programme; Human Frontier Science
Program; Oesterreichische Nationalbank Anniversary Fund 14211; Austrian Research
Promotion Agency; FemTech
author:
- first_name: Michael
full_name: Grusch, Michael
last_name: Grusch
- first_name: Karin
full_name: Schelch, Karin
last_name: Schelch
- first_name: Robert
full_name: Riedler, Robert
last_name: Riedler
- first_name: Eva
full_name: Gschaider-Reichhart, Eva
id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
last_name: Gschaider-Reichhart
orcid: 0000-0002-7218-7738
- first_name: Christopher
full_name: Differ, Christopher
last_name: Differ
- first_name: Walter
full_name: Berger, Walter
last_name: Berger
- first_name: Álvaro
full_name: Inglés Prieto, Álvaro
id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
last_name: Inglés Prieto
orcid: 0000-0002-5409-8571
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
citation:
ama: Grusch M, Schelch K, Riedler R, et al. Spatio-temporally precise activation
of engineered receptor tyrosine kinases by light. EMBO Journal. 2014;33(15):1713-1726.
doi:10.15252/embj.201387695
apa: Grusch, M., Schelch, K., Riedler, R., Gschaider-Reichhart, E., Differ, C.,
Berger, W., … Janovjak, H. L. (2014). Spatio-temporally precise activation of
engineered receptor tyrosine kinases by light. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.15252/embj.201387695
chicago: Grusch, Michael, Karin Schelch, Robert Riedler, Eva Gschaider-Reichhart,
Christopher Differ, Walter Berger, Álvaro Inglés Prieto, and Harald L Janovjak.
“Spatio-Temporally Precise Activation of Engineered Receptor Tyrosine Kinases
by Light.” EMBO Journal. Wiley-Blackwell, 2014. https://doi.org/10.15252/embj.201387695.
ieee: M. Grusch et al., “Spatio-temporally precise activation of engineered
receptor tyrosine kinases by light,” EMBO Journal, vol. 33, no. 15. Wiley-Blackwell,
pp. 1713–1726, 2014.
ista: Grusch M, Schelch K, Riedler R, Gschaider-Reichhart E, Differ C, Berger W,
Inglés Prieto Á, Janovjak HL. 2014. Spatio-temporally precise activation of engineered
receptor tyrosine kinases by light. EMBO Journal. 33(15), 1713–1726.
mla: Grusch, Michael, et al. “Spatio-Temporally Precise Activation of Engineered
Receptor Tyrosine Kinases by Light.” EMBO Journal, vol. 33, no. 15, Wiley-Blackwell,
2014, pp. 1713–26, doi:10.15252/embj.201387695.
short: M. Grusch, K. Schelch, R. Riedler, E. Gschaider-Reichhart, C. Differ, W.
Berger, Á. Inglés Prieto, H.L. Janovjak, EMBO Journal 33 (2014) 1713–1726.
date_created: 2018-12-11T11:55:37Z
date_published: 2014-07-01T00:00:00Z
date_updated: 2023-09-07T12:49:09Z
day: '01'
department:
- _id: HaJa
doi: 10.15252/embj.201387695
intvolume: ' 33'
issue: '15'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194103/
month: '07'
oa: 1
oa_version: Submitted Version
page: 1713 - 1726
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4953'
quality_controlled: '1'
related_material:
record:
- id: '418'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Spatio-temporally precise activation of engineered receptor tyrosine kinases
by light
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 33
year: '2014'
...