--- res: bibo_abstract: - Receptor tyrosine kinases (RTKs) are a large family of cell surface receptors that sense growth factors and hormones and regulate a variety of cell behaviours in health and disease. Contactless activation of RTKs with spatial and temporal precision is currently not feasible. Here, we generated RTKs that are insensitive to endogenous ligands but can be selectively activated by low-intensity blue light. We screened light-oxygen-voltage (LOV)-sensing domains for their ability to activate RTKs by light-activated dimerization. Incorporation of LOV domains found in aureochrome photoreceptors of stramenopiles resulted in robust activation of the fibroblast growth factor receptor 1 (FGFR1), epidermal growth factor receptor (EGFR) and rearranged during transfection (RET). In human cancer and endothelial cells, light induced cellular signalling with spatial and temporal precision. Furthermore, light faithfully mimicked complex mitogenic and morphogenic cell behaviour induced by growth factors. RTKs under optical control (Opto-RTKs) provide a powerful optogenetic approach to actuate cellular signals and manipulate cell behaviour.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Michael foaf_name: Grusch, Michael foaf_surname: Grusch - foaf_Person: foaf_givenName: Karin foaf_name: Schelch, Karin foaf_surname: Schelch - foaf_Person: foaf_givenName: Robert foaf_name: Riedler, Robert foaf_surname: Riedler - foaf_Person: foaf_givenName: Eva foaf_name: Gschaider-Reichhart, Eva foaf_surname: Gschaider-Reichhart foaf_workInfoHomepage: http://www.librecat.org/personId=3FEE232A-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-7218-7738 - foaf_Person: foaf_givenName: Christopher foaf_name: Differ, Christopher foaf_surname: Differ - foaf_Person: foaf_givenName: Walter foaf_name: Berger, Walter foaf_surname: Berger - foaf_Person: foaf_givenName: Álvaro foaf_name: Inglés Prieto, Álvaro foaf_surname: Inglés Prieto foaf_workInfoHomepage: http://www.librecat.org/personId=2A9DB292-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-5409-8571 - foaf_Person: foaf_givenName: Harald L foaf_name: Janovjak, Harald L foaf_surname: Janovjak foaf_workInfoHomepage: http://www.librecat.org/personId=33BA6C30-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8023-9315 bibo_doi: 10.15252/embj.201387695 bibo_issue: '15' bibo_volume: 33 dct_date: 2014^xs_gYear dct_language: eng dct_publisher: Wiley-Blackwell@ dct_title: Spatio-temporally precise activation of engineered receptor tyrosine kinases by light@ ...