--- _id: '2004' abstract: - lang: eng text: We have assembled a network of cell-fate determining transcription factors that play a key role in the specification of the ventral neuronal subtypes of the spinal cord on the basis of published transcriptional interactions. Asynchronous Boolean modelling of the network was used to compare simulation results with reported experimental observations. Such comparison highlighted the need to include additional regulatory connections in order to obtain the fixed point attractors of the model associated with the five known progenitor cell types located in the ventral spinal cord. The revised gene regulatory network reproduced previously observed cell state switches between progenitor cells observed in knock-out animal models or in experiments where the transcription factors were overexpressed. Furthermore the network predicted the inhibition of Irx3 by Nkx2.2 and this prediction was tested experimentally. Our results provide evidence for the existence of an as yet undescribed inhibitory connection which could potentially have significance beyond the ventral spinal cord. The work presented in this paper demonstrates the strength of Boolean modelling for identifying gene regulatory networks. article_number: e111430 author: - first_name: Anna full_name: Lovrics, Anna last_name: Lovrics - first_name: Yu full_name: Gao, Yu last_name: Gao - first_name: Bianka full_name: Juhász, Bianka last_name: Juhász - first_name: István full_name: Bock, István last_name: Bock - first_name: Helen full_name: Byrne, Helen last_name: Byrne - first_name: András full_name: Dinnyés, András last_name: Dinnyés - first_name: Krisztián full_name: Kovács, Krisztián id: 2AB5821E-F248-11E8-B48F-1D18A9856A87 last_name: Kovács citation: ama: Lovrics A, Gao Y, Juhász B, et al. Boolean modelling reveals new regulatory connections between transcription factors orchestrating the development of the ventral spinal cord. PLoS One. 2014;9(11). doi:10.1371/journal.pone.0111430 apa: Lovrics, A., Gao, Y., Juhász, B., Bock, I., Byrne, H., Dinnyés, A., & Kovács, K. (2014). Boolean modelling reveals new regulatory connections between transcription factors orchestrating the development of the ventral spinal cord. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0111430 chicago: Lovrics, Anna, Yu Gao, Bianka Juhász, István Bock, Helen Byrne, András Dinnyés, and Krisztián Kovács. “Boolean Modelling Reveals New Regulatory Connections between Transcription Factors Orchestrating the Development of the Ventral Spinal Cord.” PLoS One. Public Library of Science, 2014. https://doi.org/10.1371/journal.pone.0111430. ieee: A. Lovrics et al., “Boolean modelling reveals new regulatory connections between transcription factors orchestrating the development of the ventral spinal cord,” PLoS One, vol. 9, no. 11. Public Library of Science, 2014. ista: Lovrics A, Gao Y, Juhász B, Bock I, Byrne H, Dinnyés A, Kovács K. 2014. Boolean modelling reveals new regulatory connections between transcription factors orchestrating the development of the ventral spinal cord. PLoS One. 9(11), e111430. mla: Lovrics, Anna, et al. “Boolean Modelling Reveals New Regulatory Connections between Transcription Factors Orchestrating the Development of the Ventral Spinal Cord.” PLoS One, vol. 9, no. 11, e111430, Public Library of Science, 2014, doi:10.1371/journal.pone.0111430. short: A. Lovrics, Y. Gao, B. Juhász, I. Bock, H. Byrne, A. Dinnyés, K. Kovács, PLoS One 9 (2014). date_created: 2018-12-11T11:55:09Z date_published: 2014-11-14T00:00:00Z date_updated: 2023-02-23T14:06:14Z day: '14' ddc: - '570' department: - _id: JoCs doi: 10.1371/journal.pone.0111430 ec_funded: 1 file: - access_level: open_access checksum: a2289b843f7463eb1233f9ce45e6a943 content_type: application/pdf creator: system date_created: 2018-12-12T10:10:58Z date_updated: 2020-07-14T12:45:24Z file_id: '4850' file_name: IST-2016-435-v1+1_journal.pone.0111430.pdf file_size: 829363 relation: main_file file_date_updated: 2020-07-14T12:45:24Z has_accepted_license: '1' intvolume: ' 9' issue: '11' language: - iso: eng license: https://creativecommons.org/licenses/by/4.0/ month: '11' oa: 1 oa_version: Published Version project: - _id: 25681D80-B435-11E9-9278-68D0E5697425 call_identifier: FP7 grant_number: '291734' name: International IST Postdoc Fellowship Programme publication: PLoS One publication_status: published publisher: Public Library of Science publist_id: '5072' pubrep_id: '435' quality_controlled: '1' related_material: record: - id: '9722' relation: research_data status: public scopus_import: 1 status: public title: Boolean modelling reveals new regulatory connections between transcription factors orchestrating the development of the ventral spinal cord tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87 volume: 9 year: '2014' ...