--- res: bibo_abstract: - We have assembled a network of cell-fate determining transcription factors that play a key role in the specification of the ventral neuronal subtypes of the spinal cord on the basis of published transcriptional interactions. Asynchronous Boolean modelling of the network was used to compare simulation results with reported experimental observations. Such comparison highlighted the need to include additional regulatory connections in order to obtain the fixed point attractors of the model associated with the five known progenitor cell types located in the ventral spinal cord. The revised gene regulatory network reproduced previously observed cell state switches between progenitor cells observed in knock-out animal models or in experiments where the transcription factors were overexpressed. Furthermore the network predicted the inhibition of Irx3 by Nkx2.2 and this prediction was tested experimentally. Our results provide evidence for the existence of an as yet undescribed inhibitory connection which could potentially have significance beyond the ventral spinal cord. The work presented in this paper demonstrates the strength of Boolean modelling for identifying gene regulatory networks.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Anna foaf_name: Lovrics, Anna foaf_surname: Lovrics - foaf_Person: foaf_givenName: Yu foaf_name: Gao, Yu foaf_surname: Gao - foaf_Person: foaf_givenName: Bianka foaf_name: Juhász, Bianka foaf_surname: Juhász - foaf_Person: foaf_givenName: István foaf_name: Bock, István foaf_surname: Bock - foaf_Person: foaf_givenName: Helen foaf_name: Byrne, Helen foaf_surname: Byrne - foaf_Person: foaf_givenName: András foaf_name: Dinnyés, András foaf_surname: Dinnyés - foaf_Person: foaf_givenName: Krisztián foaf_name: Kovács, Krisztián foaf_surname: Kovács foaf_workInfoHomepage: http://www.librecat.org/personId=2AB5821E-F248-11E8-B48F-1D18A9856A87 bibo_doi: 10.1371/journal.pone.0111430 bibo_issue: '11' bibo_volume: 9 dct_date: 2014^xs_gYear dct_language: eng dct_publisher: Public Library of Science@ dct_title: Boolean modelling reveals new regulatory connections between transcription factors orchestrating the development of the ventral spinal cord@ ...