---
_id: '1984'
abstract:
- lang: eng
text: In animal and plant cells, mitotic chromatin locally generates microtubules
that self-organize into a mitotic spindle, and its dimensions and bipolar symmetry
are essential for accurate chromosome segregation. By immobilizing microscopic
chromatin-coated beads on slide surfaces using a microprinting technique, we have
examined the effect of chromatin on the dimensions and symmetry of spindles in
Xenopus laevis cytoplasmic extracts. While circular spots with diameters around
14-18 μm trigger bipolar spindle formation, larger spots generate an incorrect
number of poles. We also examined lines of chromatin with various dimensions.
Their length determined the number of poles that formed, with a 6 × 18 μm rectangular
patch generating normal spindle morphology. Around longer lines, multiple poles
formed and the structures were disorganized. While lines thinner than 10 μm generated
symmetric structures, thicker lines induced the formation of asymmetric structures
where all microtubules are on the same side of the line. Our results show that
chromatin defines spindle shape and orientation. For a video summary of this article,
see the PaperFlick file available with the online Supplemental Data.
acknowledgement: This work was supported by EU contract LSHG-CT-2004-503568 ComBio,
the Spanish ministry of education (M.M.C.), and EU-STREP active BioMics (A.D.).
Research in the Nedelec lab is funded by the Center for Modeling and Simulation
in the Biosciences (http://www.bioms.de), the Volkswagenstiftung, and Human Frontier
Science Program grant RGY84.
author:
- first_name: Ana
full_name: Dinarina, Ana
last_name: Dinarina
- first_name: Céline
full_name: Pugieux, Céline
last_name: Pugieux
- first_name: Maria
full_name: Corral, Maria M
last_name: Corral
- first_name: Martin
full_name: Martin Loose
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Joachim
full_name: Spatz, Joachim P
last_name: Spatz
- first_name: Éric
full_name: Karsenti, Éric
last_name: Karsenti
- first_name: François
full_name: Nédélec, François J
last_name: Nédélec
citation:
ama: Dinarina A, Pugieux C, Corral M, et al. Chromatin shapes the mitotic spindle.
Cell. 2009;138(3):502-513. doi:10.1016/j.cell.2009.05.027
apa: Dinarina, A., Pugieux, C., Corral, M., Loose, M., Spatz, J., Karsenti, É.,
& Nédélec, F. (2009). Chromatin shapes the mitotic spindle. Cell. Cell
Press. https://doi.org/10.1016/j.cell.2009.05.027
chicago: Dinarina, Ana, Céline Pugieux, Maria Corral, Martin Loose, Joachim Spatz,
Éric Karsenti, and François Nédélec. “Chromatin Shapes the Mitotic Spindle.” Cell.
Cell Press, 2009. https://doi.org/10.1016/j.cell.2009.05.027.
ieee: A. Dinarina et al., “Chromatin shapes the mitotic spindle,” Cell,
vol. 138, no. 3. Cell Press, pp. 502–513, 2009.
ista: Dinarina A, Pugieux C, Corral M, Loose M, Spatz J, Karsenti É, Nédélec F.
2009. Chromatin shapes the mitotic spindle. Cell. 138(3), 502–513.
mla: Dinarina, Ana, et al. “Chromatin Shapes the Mitotic Spindle.” Cell,
vol. 138, no. 3, Cell Press, 2009, pp. 502–13, doi:10.1016/j.cell.2009.05.027.
short: A. Dinarina, C. Pugieux, M. Corral, M. Loose, J. Spatz, É. Karsenti, F. Nédélec,
Cell 138 (2009) 502–513.
date_created: 2018-12-11T11:55:03Z
date_published: 2009-08-07T00:00:00Z
date_updated: 2021-01-12T06:54:30Z
day: '07'
doi: 10.1016/j.cell.2009.05.027
extern: 1
intvolume: ' 138'
issue: '3'
month: '08'
page: 502 - 513
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5100'
quality_controlled: 0
status: public
title: Chromatin shapes the mitotic spindle
type: journal_article
volume: 138
year: '2009'
...