---
_id: '1979'
abstract:
- lang: eng
text: NADH-ubiquinone oxidoreductase (complex I) is the first and largest enzyme
in the respiratory chain of mitochondria and many bacteria. It couples the transfer
of two electrons between NADH and ubiquinone to the translocation of four protons
across the membrane. Complex I is an L-shaped assembly formed by the hydrophilic
(peripheral) arm, containing all the redox centres performing electron transfer
and the membrane arm, containing proton-translocating machinery. Mitochondrial
complex I consists of 44 subunits of about 1 MDa in total, whilst the prokaryotic
enzyme is simpler and generally consists of 14 conserved “core” subunits. Recently
we have determined the first atomic structure of the entire complex I, using the
enzyme from Thermus thermophilus (536 kDa, 16 subunits, 9 Fe-S clusters, 64 TM
helices). Structure suggests a unique coupling mechanism, with redox energy of
electron transfer driving proton translocation via long-range (up to ~200 Å) conformational
changes. It resembles a steam engine, with coupling elements (akin to coupling
rods) linking parts of this molecular machine.
author:
- first_name: Leonid A
full_name: Leonid Sazanov
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
citation:
ama: Sazanov LA. The mechanism of coupling between electron transfer and proton
translocation in respiratory complex I. Journal of Bioenergetics and Biomembranes.
2014;46(4):247-253. doi:10.1007/s10863-014-9554-z
apa: Sazanov, L. A. (2014). The mechanism of coupling between electron transfer
and proton translocation in respiratory complex I. Journal of Bioenergetics
and Biomembranes. Springer. https://doi.org/10.1007/s10863-014-9554-z
chicago: Sazanov, Leonid A. “The Mechanism of Coupling between Electron Transfer
and Proton Translocation in Respiratory Complex I.” Journal of Bioenergetics
and Biomembranes. Springer, 2014. https://doi.org/10.1007/s10863-014-9554-z.
ieee: L. A. Sazanov, “The mechanism of coupling between electron transfer and proton
translocation in respiratory complex I,” Journal of Bioenergetics and Biomembranes,
vol. 46, no. 4. Springer, pp. 247–253, 2014.
ista: Sazanov LA. 2014. The mechanism of coupling between electron transfer and
proton translocation in respiratory complex I. Journal of Bioenergetics and Biomembranes.
46(4), 247–253.
mla: Sazanov, Leonid A. “The Mechanism of Coupling between Electron Transfer and
Proton Translocation in Respiratory Complex I.” Journal of Bioenergetics and
Biomembranes, vol. 46, no. 4, Springer, 2014, pp. 247–53, doi:10.1007/s10863-014-9554-z.
short: L.A. Sazanov, Journal of Bioenergetics and Biomembranes 46 (2014) 247–253.
date_created: 2018-12-11T11:55:01Z
date_published: 2014-08-01T00:00:00Z
date_updated: 2021-01-12T06:54:28Z
day: '01'
doi: 10.1007/s10863-014-9554-z
extern: 1
intvolume: ' 46'
issue: '4'
month: '08'
page: 247 - 253
publication: Journal of Bioenergetics and Biomembranes
publication_status: published
publisher: Springer
publist_id: '5104'
quality_controlled: 0
status: public
title: The mechanism of coupling between electron transfer and proton translocation
in respiratory complex I
type: journal_article
volume: 46
year: '2014'
...