---
_id: '1976'
abstract:
- lang: eng
text: 'Complex I is a key enzyme of the respiratory chain in many organisms. This
multi-protein complex with an intricate evolutionary history originated from the
unification of prebuilt modules of hydrogenases and transporters. Using recently
determined crystallographic structures of complex I we reanalyzed evolutionarily
related complexes that couple oxidoreduction to trans-membrane ion translocation.
Our analysis points to the previously unnoticed structural homology of the electron
input module of formate dehydrogenlyases and subunit NuoG of complex I. We also
show that all related to complex I hydrogenases likely operate via a conformation
driven mechanism with structural changes generated in the conserved coupling site
located at the interface of subunits NuoB/D/H. The coupling apparently originated
once in evolutionary history, together with subunit NuoH joining hydrogenase and
transport modules. Analysis of quinone oxidoreduction properties and the structure
of complex I allows us to suggest a fully reversible coupling mechanism. Our model
predicts that: 1) proton access to the ketone groups of the bound quinone is rigorously
controlled by the protein, 2) the negative electric charge of the anionic ubiquinol
head group is a major driving force for conformational changes.'
acknowledgement: The work in authors' laboratory was funded by the Medical Research
Council.
author:
- first_name: Rouslan
full_name: Efremov, Rouslan G
last_name: Efremov
- first_name: Leonid A
full_name: Leonid Sazanov
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
citation:
ama: Efremov R, Sazanov LA. The coupling mechanism of respiratory complex i - A
structural and evolutionary perspective. Biochimica et Biophysica Acta - Bioenergetics.
2012;1817(10):1785-1795. doi:10.1016/j.bbabio.2012.02.015
apa: Efremov, R., & Sazanov, L. A. (2012). The coupling mechanism of respiratory
complex i - A structural and evolutionary perspective. Biochimica et Biophysica
Acta - Bioenergetics. Elsevier. https://doi.org/10.1016/j.bbabio.2012.02.015
chicago: Efremov, Rouslan, and Leonid A Sazanov. “The Coupling Mechanism of Respiratory
Complex i - A Structural and Evolutionary Perspective.” Biochimica et Biophysica
Acta - Bioenergetics. Elsevier, 2012. https://doi.org/10.1016/j.bbabio.2012.02.015.
ieee: R. Efremov and L. A. Sazanov, “The coupling mechanism of respiratory complex
i - A structural and evolutionary perspective,” Biochimica et Biophysica Acta
- Bioenergetics, vol. 1817, no. 10. Elsevier, pp. 1785–1795, 2012.
ista: Efremov R, Sazanov LA. 2012. The coupling mechanism of respiratory complex
i - A structural and evolutionary perspective. Biochimica et Biophysica Acta -
Bioenergetics. 1817(10), 1785–1795.
mla: Efremov, Rouslan, and Leonid A. Sazanov. “The Coupling Mechanism of Respiratory
Complex i - A Structural and Evolutionary Perspective.” Biochimica et Biophysica
Acta - Bioenergetics, vol. 1817, no. 10, Elsevier, 2012, pp. 1785–95, doi:10.1016/j.bbabio.2012.02.015.
short: R. Efremov, L.A. Sazanov, Biochimica et Biophysica Acta - Bioenergetics 1817
(2012) 1785–1795.
date_created: 2018-12-11T11:55:00Z
date_published: 2012-10-01T00:00:00Z
date_updated: 2019-04-26T07:22:06Z
day: '01'
doi: 10.1016/j.bbabio.2012.02.015
extern: 1
intvolume: ' 1817'
issue: '10'
month: '10'
page: 1785 - 1795
publication: Biochimica et Biophysica Acta - Bioenergetics
publication_status: published
publisher: Elsevier
publist_id: '5108'
quality_controlled: 0
status: public
title: The coupling mechanism of respiratory complex i - A structural and evolutionary
perspective
type: review
volume: 1817
year: '2012'
...