{"_id":"1975","year":"2011","abstract":[{"lang":"eng","text":"Modern α-proteobacteria are thought to be closely related to the ancient symbiont of eukaryotes, an ancestor of mitochondria. Respiratory complex I from α-proteobacteria and mitochondria is well conserved at the level of the 14 &quot;core&quot; subunits, consistent with that notion. Mitochondrial complex I contains the core subunits, present in all species, and up to 31 &quot;supernumerary&quot; subunits, generally thought to have originated only within eukaryotic lineages. However, the full protein composition of an α-proteobacterial complex I has not been established previously. Here, we report the first purification and characterization of complex I from the α-proteobacterium Paracoccus denitrificans. Single particle electron microscopy shows that the complex has a well defined L-shape. Unexpectedly, in addition to the 14 core subunits, the enzyme also contains homologues of three supernumerary mitochondrial subunits as follows: B17.2, AQDQ/18, and 13 kDa (bovine nomenclature). This finding suggests that evolution of complex I via addition of supernumerary or &quot;accessory&quot; subunits started before the original endosymbiotic event that led to the creation of the eukaryotic cell. It also provides further confirmation that α-proteobacteria are the closest extant relatives of mitochondria."}],"title":"Evolution of respiratory complex I &quot;Supernumerary&quot; subunits are present in the α-proteobacterial enzyme","date_published":"2011-02-18T00:00:00Z","month":"02","doi":"10.1074/jbc.M110.194993","date_updated":"2021-01-12T06:54:27Z","author":[{"full_name":"Yip, Chui Y","first_name":"Chui","last_name":"Yip"},{"last_name":"Harbour","full_name":"Harbour, Michael E","first_name":"Michael"},{"first_name":"Kamburapola","full_name":"Jayawardena, Kamburapola G","last_name":"Jayawardena"},{"last_name":"Fearnley","full_name":"Fearnley, Ian M","first_name":"Ian"},{"last_name":"Sazanov","orcid":"0000-0002-0977-7989","full_name":"Leonid Sazanov","first_name":"Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2018-12-11T11:55:00Z","quality_controlled":0,"type":"journal_article","publication":"Journal of Biological Chemistry","acknowledgement":"This work was supported by the Medical Research Council. ","status":"public","issue":"7","citation":{"ama":"Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. Evolution of respiratory complex I &#38;quot;Supernumerary&#38;quot; subunits are present in the α-proteobacterial enzyme. <i>Journal of Biological Chemistry</i>. 2011;286(7):5023-5033. doi:<a href=\"https://doi.org/10.1074/jbc.M110.194993\">10.1074/jbc.M110.194993</a>","short":"C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, L.A. Sazanov, Journal of Biological Chemistry 286 (2011) 5023–5033.","mla":"Yip, Chui, et al. “Evolution of Respiratory Complex I &#38;quot;Supernumerary&#38;quot; Subunits Are Present in the α-Proteobacterial Enzyme.” <i>Journal of Biological Chemistry</i>, vol. 286, no. 7, American Society for Biochemistry and Molecular Biology, 2011, pp. 5023–33, doi:<a href=\"https://doi.org/10.1074/jbc.M110.194993\">10.1074/jbc.M110.194993</a>.","apa":"Yip, C., Harbour, M., Jayawardena, K., Fearnley, I., &#38; Sazanov, L. A. (2011). Evolution of respiratory complex I &#38;quot;Supernumerary&#38;quot; subunits are present in the α-proteobacterial enzyme. <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1074/jbc.M110.194993\">https://doi.org/10.1074/jbc.M110.194993</a>","ista":"Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. 2011. Evolution of respiratory complex I &#38;quot;Supernumerary&#38;quot; subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 286(7), 5023–5033.","ieee":"C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, and L. A. Sazanov, “Evolution of respiratory complex I &#38;quot;Supernumerary&#38;quot; subunits are present in the α-proteobacterial enzyme,” <i>Journal of Biological Chemistry</i>, vol. 286, no. 7. American Society for Biochemistry and Molecular Biology, pp. 5023–5033, 2011.","chicago":"Yip, Chui, Michael Harbour, Kamburapola Jayawardena, Ian Fearnley, and Leonid A Sazanov. “Evolution of Respiratory Complex I &#38;quot;Supernumerary&#38;quot; Subunits Are Present in the α-Proteobacterial Enzyme.” <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology, 2011. <a href=\"https://doi.org/10.1074/jbc.M110.194993\">https://doi.org/10.1074/jbc.M110.194993</a>."},"publist_id":"5112","publication_status":"published","publisher":"American Society for Biochemistry and Molecular Biology","page":"5023 - 5033","day":"18","volume":286,"intvolume":"       286","extern":1}