Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme

Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. 2011. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 286(7), 5023–5033.

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Journal Article | Published
Author
Yip, Chui Y; Harbour, Michael E; Jayawardena, Kamburapola G; Fearnley, Ian M; Sazanov, Leonid AIST Austria
Abstract
Modern α-proteobacteria are thought to be closely related to the ancient symbiont of eukaryotes, an ancestor of mitochondria. Respiratory complex I from α-proteobacteria and mitochondria is well conserved at the level of the 14 "core" subunits, consistent with that notion. Mitochondrial complex I contains the core subunits, present in all species, and up to 31 "supernumerary" subunits, generally thought to have originated only within eukaryotic lineages. However, the full protein composition of an α-proteobacterial complex I has not been established previously. Here, we report the first purification and characterization of complex I from the α-proteobacterium Paracoccus denitrificans. Single particle electron microscopy shows that the complex has a well defined L-shape. Unexpectedly, in addition to the 14 core subunits, the enzyme also contains homologues of three supernumerary mitochondrial subunits as follows: B17.2, AQDQ/18, and 13 kDa (bovine nomenclature). This finding suggests that evolution of complex I via addition of supernumerary or "accessory" subunits started before the original endosymbiotic event that led to the creation of the eukaryotic cell. It also provides further confirmation that α-proteobacteria are the closest extant relatives of mitochondria.
Publishing Year
Date Published
2011-02-18
Journal Title
Journal of Biological Chemistry
Acknowledgement
This work was supported by the Medical Research Council.
Volume
286
Issue
7
Page
5023 - 5033
IST-REx-ID

Cite this

Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 2011;286(7):5023-5033. doi:10.1074/jbc.M110.194993
Yip, C., Harbour, M., Jayawardena, K., Fearnley, I., & Sazanov, L. A. (2011). Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M110.194993
Yip, Chui, Michael Harbour, Kamburapola Jayawardena, Ian Fearnley, and Leonid A Sazanov. “Evolution of Respiratory Complex I "Supernumerary" Subunits Are Present in the α-Proteobacterial Enzyme.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2011. https://doi.org/10.1074/jbc.M110.194993.
C. Yip, M. Harbour, K. Jayawardena, I. Fearnley, and L. A. Sazanov, “Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme,” Journal of Biological Chemistry, vol. 286, no. 7. American Society for Biochemistry and Molecular Biology, pp. 5023–5033, 2011.
Yip C, Harbour M, Jayawardena K, Fearnley I, Sazanov LA. 2011. Evolution of respiratory complex I "Supernumerary" subunits are present in the α-proteobacterial enzyme. Journal of Biological Chemistry. 286(7), 5023–5033.
Yip, Chui, et al. “Evolution of Respiratory Complex I "Supernumerary" Subunits Are Present in the α-Proteobacterial Enzyme.” Journal of Biological Chemistry, vol. 286, no. 7, American Society for Biochemistry and Molecular Biology, 2011, pp. 5023–33, doi:10.1074/jbc.M110.194993.

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