Efremov, Rouslan G; Sazanov, Leonid AIST Austria
Outer membrane protein F, a major component of the Escherichia coli outer membrane, was crystallized for the first time in lipidic mesophase of monoolein in novel space groups, P1 and H32. Due to ease of its purification and crystallization OmpF can be used as a benchmark protein for establishing membrane protein crystallization in meso, as a "membrane lyzozyme" The packing of porin trimers in the crystals of space group H32 is similar to natural outer membranes, providing the first high-resolution insight into the close to native packing of OmpF. Surprisingly, interaction between trimers is mediated exclusively by lipids, without direct protein-protein contacts. Multiple ordered lipids are observed and many of them occupy identical positions independently of the space group, identifying preferential interaction sites of lipid acyl chains. Presence of ordered aliphatic chains close to a positively charged area on the porin surface suggests a position for a lipopolysaccharide binding site on the surface of the major E. coli porins.
Journal of Structural Biology
This work was funded by the Medical Research Council.
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Efremov R, Sazanov LA. Structure of Escherichia coli OmpF porin from lipidic mesophase. Journal of Structural Biology. 2012;178(3):311-318. doi:10.1016/j.jsb.2012.03.005
Efremov, R., & Sazanov, L. A. (2012). Structure of Escherichia coli OmpF porin from lipidic mesophase. Journal of Structural Biology, 178(3), 311–318. https://doi.org/10.1016/j.jsb.2012.03.005
Efremov, Rouslan, and Leonid A Sazanov. “Structure of Escherichia Coli OmpF Porin from Lipidic Mesophase.” Journal of Structural Biology 178, no. 3 (2012): 311–18. https://doi.org/10.1016/j.jsb.2012.03.005.
R. Efremov and L. A. Sazanov, “Structure of Escherichia coli OmpF porin from lipidic mesophase,” Journal of Structural Biology, vol. 178, no. 3, pp. 311–318, 2012.
Efremov R, Sazanov LA. 2012. Structure of Escherichia coli OmpF porin from lipidic mesophase. Journal of Structural Biology. 178(3), 311–318.
Efremov, Rouslan, and Leonid A. Sazanov. “Structure of Escherichia Coli OmpF Porin from Lipidic Mesophase.” Journal of Structural Biology, vol. 178, no. 3, Academic Press, 2012, pp. 311–18, doi:10.1016/j.jsb.2012.03.005.