Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I

Baranova E, Morgan D, Sazanov LA. 2007. Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I. Journal of Structural Biology. 159(2 SPEC. ISS.), 238–242.

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Journal Article | Published
Author
Baranova, Ekaterina A; Morgan, David J; Sazanov, Leonid AIST Austria
Abstract
Respiratory complex I catalyses the transfer of electrons from NADH to quinone coupled to the translocation of protons across the membrane. The mechanism of coupling and the structure of the complete enzyme are not known. The membrane domain of the complex contains three similar antiporter-like subunits NuoL/M/N, probably involved in proton pumping. We have previously shown that subunits NuoL/M can be removed from the rest of the complex, suggesting their location at the distal end of the membrane domain. Here, using electron microscopy and single particle analysis, we show that subunits NuoL and M jointly occupy a distal half of the membrane domain, separated by about 10 nm from the interface with the peripheral arm. This indicates that coupling mechanism of complex I is likely to involve long range conformational changes.
Publishing Year
Date Published
2007-08-01
Journal Title
Journal of Structural Biology
Volume
159
Issue
2 SPEC. ISS.
Page
238 - 242
IST-REx-ID

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Baranova E, Morgan D, Sazanov LA. Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I. Journal of Structural Biology. 2007;159(2 SPEC. ISS.):238-242. doi:10.1016/j.jsb.2007.01.009
Baranova, E., Morgan, D., & Sazanov, L. A. (2007). Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I. Journal of Structural Biology. Academic Press. https://doi.org/10.1016/j.jsb.2007.01.009
Baranova, Ekaterina, David Morgan, and Leonid A Sazanov. “Single Particle Analysis Confirms Distal Location of Subunits NuoL and NuoM in Escherichia Coli Complex I.” Journal of Structural Biology. Academic Press, 2007. https://doi.org/10.1016/j.jsb.2007.01.009.
E. Baranova, D. Morgan, and L. A. Sazanov, “Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I,” Journal of Structural Biology, vol. 159, no. 2 SPEC. ISS. Academic Press, pp. 238–242, 2007.
Baranova E, Morgan D, Sazanov LA. 2007. Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I. Journal of Structural Biology. 159(2 SPEC. ISS.), 238–242.
Baranova, Ekaterina, et al. “Single Particle Analysis Confirms Distal Location of Subunits NuoL and NuoM in Escherichia Coli Complex I.” Journal of Structural Biology, vol. 159, no. 2 SPEC. ISS., Academic Press, 2007, pp. 238–42, doi:10.1016/j.jsb.2007.01.009.

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