Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus

L.A. Sazanov, P. Hinchliffe, Science 311 (2006) 1430–1436.

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Abstract
Respiratory complex I plays a central role in cellular energy production in bacteria and mitochondria. Its dysfunction is implicated in many human neurodegenerative diseases, as well as in aging. The crystal structure of the hydrophilic domain (peripheral arm) of complex I from Thermus thermophilus has been solved at 3.3 angstrom resolution. This subcomplex consists of eight subunits and contains all the redox centers of the enzyme, including nine iron-sulfur clusters. The primary electron acceptor, flavin-mononucleotide, is within electron transfer distance of cluster N3, leading to the main redox pathway, and of the distal cluster Nia, a possible antioxidant. The structure reveals new aspects of the mechanism and evolution of the enzyme. The terminal cluster N2 is coordinated, uniquely, by two consecutive cysteines. The novel subunit Nqo15 has a similar fold to the mitochondrial iron chaperone frataxin, and it may be involved in iron-sulfur cluster regeneration in the complex.
Publishing Year
Date Published
2006-03-10
Journal Title
Science
Acknowledgement
This work was funded by the Medical Research Council.
Volume
311
Issue
5766
Page
1430 - 1436
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Sazanov LA, Hinchliffe P. Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus. Science. 2006;311(5766):1430-1436. doi:10.1126/science.1123809
Sazanov, L. A., & Hinchliffe, P. (2006). Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus. Science, 311(5766), 1430–1436. https://doi.org/10.1126/science.1123809
Sazanov, Leonid A, and Philip Hinchliffe. “Structure of the Hydrophilic Domain of Respiratory Complex I from Thermus Thermophilus.” Science 311, no. 5766 (2006): 1430–36. https://doi.org/10.1126/science.1123809.
L. A. Sazanov and P. Hinchliffe, “Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus,” Science, vol. 311, no. 5766, pp. 1430–1436, 2006.
Sazanov LA, Hinchliffe P. 2006. Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus. Science. 311(5766), 1430–1436.
Sazanov, Leonid A., and Philip Hinchliffe. “Structure of the Hydrophilic Domain of Respiratory Complex I from Thermus Thermophilus.” Science, vol. 311, no. 5766, American Association for the Advancement of Science, 2006, pp. 1430–36, doi:10.1126/science.1123809.

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