{"_id":"1952","extern":1,"status":"public","year":"1996","type":"journal_article","month":"01","day":"11","quality_controlled":0,"publication":"Biochimica et Biophysica Acta - Bioenergetics","title":"Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme","issue":"1","abstract":[{"text":"Two strains of Rhodospirillum rubrum were constructed in which, by a gene dosage effect, the transhydrogenase activity of isolated chromatophores was increased 7-10-fold and 15-20-fold, respectively. The H+/H- ratio (the ratio of protons translocated per hydride ion equivalent transferred from NADPH to an NAD+ analogue, acetyl pyridine adenine dinucleotide), determined by a spectroscopic technique, was approximately 1.0 for chromatophores from the over-expressing strains, but was only approximately 0.6 for wild-type chromatophores. Highly-coupled proteoliposomes were prepared containing purified transhydrogenase from beef-heart mitochondria. Using the same technique, the H+/H- ratio was close to 1.0 for these proteoliposomes. It is suggested that the mechanistic H+/H- ratio is indeed unity, but that a low ratio is obtained in wild-type chromatophores because of inhomogeneity in the vesicle population.","lang":"eng"}],"publisher":"Elsevier","intvolume":"      1273","page":"4 - 12","doi":"10.1016/0005-2728(95)00125-5","publication_status":"published","date_updated":"2021-01-12T06:54:18Z","date_published":"1996-01-11T00:00:00Z","author":[{"last_name":"Bizouarn","first_name":"Tania","full_name":"Bizouarn, Tania"},{"first_name":"Leonid A","full_name":"Leonid Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989","last_name":"Sazanov"},{"first_name":"Sébastien","full_name":"Aubourg, Sébastien","last_name":"Aubourg"},{"last_name":"Jackson","full_name":"Jackson, Julie B","first_name":"Julie"}],"volume":1273,"date_created":"2018-12-11T11:54:53Z","citation":{"mla":"Bizouarn, Tania, et al. “Estimation of the H+/H- Ratio of the Reaction Catalysed by the Nicotinamide Nucleotide Transhydrogenase in Chromatophores from over-Expressing Strains of Rhodospirillum Rubrum and in Liposomes Inlaid with the Purified Bovine Enzyme.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1273, no. 1, Elsevier, 1996, pp. 4–12, doi:<a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">10.1016/0005-2728(95)00125-5</a>.","short":"T. Bizouarn, L.A. Sazanov, S. Aubourg, J. Jackson, Biochimica et Biophysica Acta - Bioenergetics 1273 (1996) 4–12.","ieee":"T. Bizouarn, L. A. Sazanov, S. Aubourg, and J. Jackson, “Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme,” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1273, no. 1. Elsevier, pp. 4–12, 1996.","chicago":"Bizouarn, Tania, Leonid A Sazanov, Sébastien Aubourg, and Julie Jackson. “Estimation of the H+/H- Ratio of the Reaction Catalysed by the Nicotinamide Nucleotide Transhydrogenase in Chromatophores from over-Expressing Strains of Rhodospirillum Rubrum and in Liposomes Inlaid with the Purified Bovine Enzyme.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier, 1996. <a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">https://doi.org/10.1016/0005-2728(95)00125-5</a>.","apa":"Bizouarn, T., Sazanov, L. A., Aubourg, S., &#38; Jackson, J. (1996). Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier. <a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">https://doi.org/10.1016/0005-2728(95)00125-5</a>","ama":"Bizouarn T, Sazanov LA, Aubourg S, Jackson J. Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. 1996;1273(1):4-12. doi:<a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">10.1016/0005-2728(95)00125-5</a>","ista":"Bizouarn T, Sazanov LA, Aubourg S, Jackson J. 1996. Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. Biochimica et Biophysica Acta - Bioenergetics. 1273(1), 4–12."},"publist_id":"5132"}