Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme

T. Bizouarn, L.A. Sazanov, S. Aubourg, J. Jackson, Biochimica et Biophysica Acta - Bioenergetics 1273 (1996) 4–12.

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Bizouarn, Tania; Sazanov, Leonid AIST Austria ; Aubourg, Sébastien; Jackson, Julie B
Abstract
Two strains of Rhodospirillum rubrum were constructed in which, by a gene dosage effect, the transhydrogenase activity of isolated chromatophores was increased 7-10-fold and 15-20-fold, respectively. The H+/H- ratio (the ratio of protons translocated per hydride ion equivalent transferred from NADPH to an NAD+ analogue, acetyl pyridine adenine dinucleotide), determined by a spectroscopic technique, was approximately 1.0 for chromatophores from the over-expressing strains, but was only approximately 0.6 for wild-type chromatophores. Highly-coupled proteoliposomes were prepared containing purified transhydrogenase from beef-heart mitochondria. Using the same technique, the H+/H- ratio was close to 1.0 for these proteoliposomes. It is suggested that the mechanistic H+/H- ratio is indeed unity, but that a low ratio is obtained in wild-type chromatophores because of inhomogeneity in the vesicle population.
Publishing Year
Date Published
1996-01-11
Journal Title
Biochimica et Biophysica Acta - Bioenergetics
Volume
1273
Issue
1
Page
4 - 12
IST-REx-ID

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Bizouarn T, Sazanov LA, Aubourg S, Jackson J. Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. Biochimica et Biophysica Acta - Bioenergetics. 1996;1273(1):4-12. doi:10.1016/0005-2728(95)00125-5
Bizouarn, T., Sazanov, L. A., Aubourg, S., & Jackson, J. (1996). Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. Biochimica et Biophysica Acta - Bioenergetics, 1273(1), 4–12. https://doi.org/10.1016/0005-2728(95)00125-5
Bizouarn, Tania, Leonid A Sazanov, Sébastien Aubourg, and Julie Jackson. “Estimation of the H+/H- Ratio of the Reaction Catalysed by the Nicotinamide Nucleotide Transhydrogenase in Chromatophores from over-Expressing Strains of Rhodospirillum Rubrum and in Liposomes Inlaid with the Purified Bovine Enzyme.” Biochimica et Biophysica Acta - Bioenergetics 1273, no. 1 (1996): 4–12. https://doi.org/10.1016/0005-2728(95)00125-5.
T. Bizouarn, L. A. Sazanov, S. Aubourg, and J. Jackson, “Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme,” Biochimica et Biophysica Acta - Bioenergetics, vol. 1273, no. 1, pp. 4–12, 1996.
Bizouarn T, Sazanov LA, Aubourg S, Jackson J. 1996. Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. Biochimica et Biophysica Acta - Bioenergetics. 1273(1), 4–12.
Bizouarn, Tania, et al. “Estimation of the H+/H- Ratio of the Reaction Catalysed by the Nicotinamide Nucleotide Transhydrogenase in Chromatophores from over-Expressing Strains of Rhodospirillum Rubrum and in Liposomes Inlaid with the Purified Bovine Enzyme.” Biochimica et Biophysica Acta - Bioenergetics, vol. 1273, no. 1, Elsevier, 1996, pp. 4–12, doi:10.1016/0005-2728(95)00125-5.

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