---
_id: '1921'
abstract:
- lang: eng
text: Cell polarity manifested by asymmetric distribution of cargoes, such as receptors
and transporters, within the plasma membrane (PM) is crucial for essential functions
in multicellular organisms. In plants, cell polarity (re)establishment is intimately
linked to patterning processes. Despite the importance of cell polarity, its underlying
mechanisms are still largely unknown, including the definition and distinctiveness
of the polar domains within the PM. Here, we show in Arabidopsis thaliana that
the signaling membrane components, the phosphoinositides phosphatidylinositol
4-phosphate (PtdIns4P) and phosphatidylinositol 4, 5-bisphosphate [PtdIns(4, 5)P2]
as well as PtdIns4P 5-kinases mediating their interconversion, are specifically
enriched at apical and basal polar plasma membrane domains. The PtdIns4P 5-kinases
PIP5K1 and PIP5K2 are redundantly required for polar localization of specifically
apical and basal cargoes, such as PIN-FORMED transporters for the plant hormone
auxin. As a consequence of the polarity defects, instructive auxin gradients as
well as embryonic and postembryonic patterning are severely compromised. Furthermore,
auxin itself regulates PIP5K transcription and PtdIns4P and PtdIns(4, 5)P2 levels,
in particular their association with polar PM domains. Our results provide insight
into the polar domain-delineating mechanisms in plant cells that depend on apical
and basal distribution of membrane lipids and are essential for embryonic and
postembryonic patterning.
acknowledgement: This work was supported by grants from the Odysseus program of the
Research Foundation-Flanders (to J.F.).
author:
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: 'MiriamPalacios '
full_name: 'Palacios-Gomez, MiriamPalacios '
last_name: Palacios-Gomez
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Mareike
full_name: Heilmann, Mareike
last_name: Heilmann
- first_name: Ringo
full_name: Van Wijk, Ringo
last_name: Van Wijk
- first_name: Joop
full_name: Vermeer, Joop
last_name: Vermeer
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Teun
full_name: Munnik, Teun
last_name: Munnik
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tejos R, Sauer M, Vanneste S, et al. Bipolar plasma membrane distribution of
phosphoinositides and their requirement for auxin-mediated cell polarity and patterning
in Arabidopsis. Plant Cell. 2014;26(5):2114-2128. doi:10.1105/tpc.114.126185
apa: Tejos, R., Sauer, M., Vanneste, S., Palacios-Gomez, M., Li, H., Heilmann, M.,
… Friml, J. (2014). Bipolar plasma membrane distribution of phosphoinositides
and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis.
Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.114.126185
chicago: Tejos, Ricardo, Michael Sauer, Steffen Vanneste, MiriamPalacios Palacios-Gomez,
Hongjiang Li, Mareike Heilmann, Ringo Van Wijk, et al. “Bipolar Plasma Membrane
Distribution of Phosphoinositides and Their Requirement for Auxin-Mediated Cell
Polarity and Patterning in Arabidopsis.” Plant Cell. American Society of
Plant Biologists, 2014. https://doi.org/10.1105/tpc.114.126185.
ieee: R. Tejos et al., “Bipolar plasma membrane distribution of phosphoinositides
and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis,”
Plant Cell, vol. 26, no. 5. American Society of Plant Biologists, pp. 2114–2128,
2014.
ista: Tejos R, Sauer M, Vanneste S, Palacios-Gomez M, Li H, Heilmann M, Van Wijk
R, Vermeer J, Heilmann I, Munnik T, Friml J. 2014. Bipolar plasma membrane distribution
of phosphoinositides and their requirement for auxin-mediated cell polarity and
patterning in Arabidopsis. Plant Cell. 26(5), 2114–2128.
mla: Tejos, Ricardo, et al. “Bipolar Plasma Membrane Distribution of Phosphoinositides
and Their Requirement for Auxin-Mediated Cell Polarity and Patterning in Arabidopsis.”
Plant Cell, vol. 26, no. 5, American Society of Plant Biologists, 2014,
pp. 2114–28, doi:10.1105/tpc.114.126185.
short: R. Tejos, M. Sauer, S. Vanneste, M. Palacios-Gomez, H. Li, M. Heilmann, R.
Van Wijk, J. Vermeer, I. Heilmann, T. Munnik, J. Friml, Plant Cell 26 (2014) 2114–2128.
date_created: 2018-12-11T11:54:43Z
date_published: 2014-05-01T00:00:00Z
date_updated: 2021-01-12T06:54:05Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.114.126185
ec_funded: 1
intvolume: ' 26'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079372/
month: '05'
oa: 1
oa_version: Submitted Version
page: 2114 - 2128
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '5173'
scopus_import: 1
status: public
title: Bipolar plasma membrane distribution of phosphoinositides and their requirement
for auxin-mediated cell polarity and patterning in Arabidopsis
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 26
year: '2014'
...