---
_id: '1865'
abstract:
- lang: eng
text: The plant hormone auxin and its directional transport are known to play a
crucial role in defining the embryonic axis and subsequent development of the
body plan. Although the role of PIN auxin efflux transporters has been clearly
assigned during embryonic shoot and root specification, the role of the auxin
influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here,
we used chemical and genetic tools on Brassica napus microspore-derived embryos
and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and
LAX2 are required for both shoot and root pole formation, in concert with PIN
efflux carriers. Furthermore, we uncovered a positive-feedback loop betweenMONOPTEROS(ARF5)-dependent
auxin signalling and auxin transport. ThisMONOPTEROSdependent transcriptional
regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4)
carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip.
These results indicate that auxin-dependent cell specification during embryo development
requires balanced auxin transport involving both influx and efflux mechanisms,
and that this transport is maintained by a positive transcriptional feedback on
auxin signalling.
acknowledgement: W.G. is a post-doctoral fellow of the Research Foundation Flanders.
H.S.R. is supported by Employment of Best Young Scientists for International Cooperation
Empowerment [CZ.1.07/2.3.00/30.0037], co-financed by the European Social Fund and
the state budget of the Czech Republic. Mi.S. was funded by the Ramón y Cajal program.
This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP],
project ‘CEITEC – Central European Institute of Technology’ [CZ.1.05/1.1.00/02.0068],
the European Social Fund [CZ.1.07/2.3.00/20.0043] and the Czech Science Foundation
GACR [GA13-40637S] to J.F. We acknowledge funding from the Biological and Biotechnological
Science Research Council (BBSRC) and Engineering Physics Science Research Council
(EPSRC) to R.S. and M.B
author:
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Mercedes
full_name: Soriano, Mercedes
last_name: Soriano
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
- first_name: Kim
full_name: Boutilier, Kim
last_name: Boutilier
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Robert H, Grunewald W, Sauer M, et al. Plant embryogenesis requires AUX/LAX-mediated
auxin influx. Development. 2015;142(4):702-711. doi:10.1242/dev.115832
apa: Robert, H., Grunewald, W., Sauer, M., Cannoot, B., Soriano, M., Swarup, R.,
… Friml, J. (2015). Plant embryogenesis requires AUX/LAX-mediated auxin influx.
Development. Company of Biologists. https://doi.org/10.1242/dev.115832
chicago: Robert, Hélène, Wim Grunewald, Michael Sauer, Bernard Cannoot, Mercedes
Soriano, Ranjan Swarup, Dolf Weijers, Malcolm Bennett, Kim Boutilier, and Jiří
Friml. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin Influx.” Development.
Company of Biologists, 2015. https://doi.org/10.1242/dev.115832.
ieee: H. Robert et al., “Plant embryogenesis requires AUX/LAX-mediated auxin
influx,” Development, vol. 142, no. 4. Company of Biologists, pp. 702–711,
2015.
ista: Robert H, Grunewald W, Sauer M, Cannoot B, Soriano M, Swarup R, Weijers D,
Bennett M, Boutilier K, Friml J. 2015. Plant embryogenesis requires AUX/LAX-mediated
auxin influx. Development. 142(4), 702–711.
mla: Robert, Hélène, et al. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin
Influx.” Development, vol. 142, no. 4, Company of Biologists, 2015, pp.
702–11, doi:10.1242/dev.115832.
short: H. Robert, W. Grunewald, M. Sauer, B. Cannoot, M. Soriano, R. Swarup, D.
Weijers, M. Bennett, K. Boutilier, J. Friml, Development 142 (2015) 702–711.
date_created: 2018-12-11T11:54:26Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:53:43Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.115832
ec_funded: 1
intvolume: ' 142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 702 - 711
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5231'
quality_controlled: '1'
scopus_import: 1
status: public
title: Plant embryogenesis requires AUX/LAX-mediated auxin influx
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...