--- _id: '1715' abstract: - lang: eng text: 'Background: Cell-to-cell communication at the synapse involves synaptic transmission as well as signaling mediated by growth factors, which provide developmental and plasticity cues. There is evidence that a retrograde, presynaptic transforming growth factor-β (TGF-β) signaling event regulates synapse development and function in Drosophila. Results: Here we show that a postsynaptic TGF-β signaling event occurs during larval development. The type I receptor Thick veins (Tkv) and the R-Smad transcription factor Mothers-against-dpp (Mad) are localized postsynaptically in the muscle. Furthermore, Mad phosphorylation occurs in regions facing the presynaptic active zones of neurotransmitter release within the postsynaptic subsynaptic reticulum (SSR). In order to monitor in real time the levels of TGF-β signaling in the synapse during synaptic transmission, we have established a FRAP assay to measure Mad nuclear import/export in the muscle. We show that Mad nuclear trafficking depends on stimulation of the muscle. Conclusions: Our data suggest a mechanism linking synaptic transmission and postsynaptic TGF-β signaling that may coordinate nerve-muscle development and function.' acknowledgement: This work was supported by the Max Planck Society, HFSP, and Deutsche Forschungsgemeinschaft. article_processing_charge: No author: - first_name: Veronika full_name: Dudu, Veronika last_name: Dudu - first_name: Thomas full_name: Bittig, Thomas last_name: Bittig - first_name: Eugeni full_name: Entchev, Eugeni last_name: Entchev - first_name: Anna full_name: Kicheva, Anna id: 3959A2A0-F248-11E8-B48F-1D18A9856A87 last_name: Kicheva orcid: 0000-0003-4509-4998 - first_name: Frank full_name: Julicher, Frank last_name: Julicher - first_name: Marcos full_name: González Gaitán, Marcos last_name: González Gaitán citation: ama: Dudu V, Bittig T, Entchev E, Kicheva A, Julicher F, González Gaitán M. Postsynaptic mad signaling at the Drosophila neuromuscular junction. Current Biology. 2006;16(7):625-635. doi:10.1016/j.cub.2006.02.061 apa: Dudu, V., Bittig, T., Entchev, E., Kicheva, A., Julicher, F., & González Gaitán, M. (2006). Postsynaptic mad signaling at the Drosophila neuromuscular junction. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2006.02.061 chicago: Dudu, Veronika, Thomas Bittig, Eugeni Entchev, Anna Kicheva, Frank Julicher, and Marcos González Gaitán. “Postsynaptic Mad Signaling at the Drosophila Neuromuscular Junction.” Current Biology. Cell Press, 2006. https://doi.org/10.1016/j.cub.2006.02.061. ieee: V. Dudu, T. Bittig, E. Entchev, A. Kicheva, F. Julicher, and M. González Gaitán, “Postsynaptic mad signaling at the Drosophila neuromuscular junction,” Current Biology, vol. 16, no. 7. Cell Press, pp. 625–635, 2006. ista: Dudu V, Bittig T, Entchev E, Kicheva A, Julicher F, González Gaitán M. 2006. Postsynaptic mad signaling at the Drosophila neuromuscular junction. Current Biology. 16(7), 625–635. mla: Dudu, Veronika, et al. “Postsynaptic Mad Signaling at the Drosophila Neuromuscular Junction.” Current Biology, vol. 16, no. 7, Cell Press, 2006, pp. 625–35, doi:10.1016/j.cub.2006.02.061. short: V. Dudu, T. Bittig, E. Entchev, A. Kicheva, F. Julicher, M. González Gaitán, Current Biology 16 (2006) 625–635. date_created: 2018-12-11T11:53:37Z date_published: 2006-04-04T00:00:00Z date_updated: 2021-11-16T07:44:15Z day: '04' doi: 10.1016/j.cub.2006.02.061 extern: '1' intvolume: ' 16' issue: '7' language: - iso: eng month: '04' oa_version: None page: 625 - 635 publication: Current Biology publication_status: published publisher: Cell Press publist_id: '5416' related_material: link: - relation: erratum url: https://doi.org/10.1016/j.cub.2006.06.020 status: public title: Postsynaptic mad signaling at the Drosophila neuromuscular junction type: journal_article user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9 volume: 16 year: '2006' ...