---
_id: '1663'
abstract:
- lang: eng
text: CREB-binding protein (CBP) and p300 are transcriptional coactivators involved
in numerous biological processes that affect cell growth, transformation, differentiation,
and development. In this study, we provide evidence of the involvement of homeodomain-interacting
protein kinase 2 (HIPK2) in the regulation of CBP activity. We show that HIPK2
interacts with and phosphorylates several regions of CBP. We demonstrate that
serines 2361, 2363, 2371, 2376, and 2381 are responsible for the HIPK2-induced
mobility shift of CBP C-terminal activation domain. Moreover, we show that HIPK2
strongly potentiates the transcriptional activity of CBP. However, our data suggest
that HIPK2 activates CBP mainly by counteracting the repressive action of cell
cycle regulatory domain 1 (CRD1), located between amino acids 977 and 1076, independently
of CBP phosphorylation. Our findings thus highlight a complex regulation of CBP
activity by HIPK2, which might be relevant for the control of specific sets of
target genes involved in cellular proliferation, differentiation and apoptosis.
author:
- first_name: Krisztián
full_name: Kovács, Krisztián
id: 2AB5821E-F248-11E8-B48F-1D18A9856A87
last_name: Kovács
- first_name: Myriam
full_name: Steinmann, Myriam
last_name: Steinmann
- first_name: Olivier
full_name: Halfon, Olivier
last_name: Halfon
- first_name: Pierre
full_name: Magistretti, Pierre
last_name: Magistretti
- first_name: Jean
full_name: Cardinaux, Jean
last_name: Cardinaux
citation:
ama: Kovács K, Steinmann M, Halfon O, Magistretti P, Cardinaux J. Complex regulation
of CREB-binding protein by homeodomain-interacting protein kinase 2. Cellular
Signalling. 2015;27(11):2252-2260. doi:10.1016/j.cellsig.2015.08.001
apa: Kovács, K., Steinmann, M., Halfon, O., Magistretti, P., & Cardinaux, J.
(2015). Complex regulation of CREB-binding protein by homeodomain-interacting
protein kinase 2. Cellular Signalling. Elsevier. https://doi.org/10.1016/j.cellsig.2015.08.001
chicago: Kovács, Krisztián, Myriam Steinmann, Olivier Halfon, Pierre Magistretti,
and Jean Cardinaux. “Complex Regulation of CREB-Binding Protein by Homeodomain-Interacting
Protein Kinase 2.” Cellular Signalling. Elsevier, 2015. https://doi.org/10.1016/j.cellsig.2015.08.001.
ieee: K. Kovács, M. Steinmann, O. Halfon, P. Magistretti, and J. Cardinaux, “Complex
regulation of CREB-binding protein by homeodomain-interacting protein kinase 2,”
Cellular Signalling, vol. 27, no. 11. Elsevier, pp. 2252–2260, 2015.
ista: Kovács K, Steinmann M, Halfon O, Magistretti P, Cardinaux J. 2015. Complex
regulation of CREB-binding protein by homeodomain-interacting protein kinase 2.
Cellular Signalling. 27(11), 2252–2260.
mla: Kovács, Krisztián, et al. “Complex Regulation of CREB-Binding Protein by Homeodomain-Interacting
Protein Kinase 2.” Cellular Signalling, vol. 27, no. 11, Elsevier, 2015,
pp. 2252–60, doi:10.1016/j.cellsig.2015.08.001.
short: K. Kovács, M. Steinmann, O. Halfon, P. Magistretti, J. Cardinaux, Cellular
Signalling 27 (2015) 2252–2260.
date_created: 2018-12-11T11:53:20Z
date_published: 2015-11-01T00:00:00Z
date_updated: 2021-01-12T06:52:22Z
day: '01'
ddc:
- '570'
department:
- _id: JoCs
doi: 10.1016/j.cellsig.2015.08.001
ec_funded: 1
file:
- access_level: local
checksum: 4ee690b6444b7a43523237f0941457d1
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:03Z
date_updated: 2020-07-14T12:45:10Z
file_id: '5321'
file_name: IST-2016-578-v1+1_CLS-D-15-00072R1_.pdf
file_size: 1735337
relation: main_file
file_date_updated: 2020-07-14T12:45:10Z
has_accepted_license: '1'
intvolume: ' 27'
issue: '11'
language:
- iso: eng
month: '11'
oa_version: Published Version
page: 2252 - 2260
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Cellular Signalling
publication_status: published
publisher: Elsevier
publist_id: '5487'
pubrep_id: '578'
quality_controlled: '1'
scopus_import: 1
status: public
title: Complex regulation of CREB-binding protein by homeodomain-interacting protein
kinase 2
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 27
year: '2015'
...