--- _id: '1623' abstract: - lang: eng text: "Background\r\nPhotosynthetic cyanobacteria are attractive for a range of biotechnological applications including biofuel production. However, due to slow growth, screening of mutant libraries using microtiter plates is not feasible.\r\nResults\r\nWe present a method for high-throughput, single-cell analysis and sorting of genetically engineered l-lactate-producing strains of Synechocystis sp. PCC6803. A microfluidic device is used to encapsulate single cells in picoliter droplets, assay the droplets for l-lactate production, and sort strains with high productivity. We demonstrate the separation of low- and high-producing reference strains, as well as enrichment of a more productive l-lactate-synthesizing population after UV-induced mutagenesis. The droplet platform also revealed population heterogeneity in photosynthetic growth and lactate production, as well as the presence of metabolically stalled cells.\r\nConclusions\r\nThe workflow will facilitate metabolic engineering and directed evolution studies and will be useful in studies of cyanobacteria biochemistry and physiology.\r\n" article_number: '193' author: - first_name: Petter full_name: Hammar, Petter last_name: Hammar - first_name: Andreas full_name: Angermayr, Andreas id: 4677C796-F248-11E8-B48F-1D18A9856A87 last_name: Angermayr orcid: 0000-0001-8619-2223 - first_name: Staffan full_name: Sjostrom, Staffan last_name: Sjostrom - first_name: Josefin full_name: Van Der Meer, Josefin last_name: Van Der Meer - first_name: Klaas full_name: Hellingwerf, Klaas last_name: Hellingwerf - first_name: Elton full_name: Hudson, Elton last_name: Hudson - first_name: Hakaan full_name: Joensson, Hakaan last_name: Joensson citation: ama: Hammar P, Angermayr A, Sjostrom S, et al. Single-cell screening of photosynthetic growth and lactate production by cyanobacteria. Biotechnology for Biofuels. 2015;8(1). doi:10.1186/s13068-015-0380-2 apa: Hammar, P., Angermayr, A., Sjostrom, S., Van Der Meer, J., Hellingwerf, K., Hudson, E., & Joensson, H. (2015). Single-cell screening of photosynthetic growth and lactate production by cyanobacteria. Biotechnology for Biofuels. BioMed Central. https://doi.org/10.1186/s13068-015-0380-2 chicago: Hammar, Petter, Andreas Angermayr, Staffan Sjostrom, Josefin Van Der Meer, Klaas Hellingwerf, Elton Hudson, and Hakaan Joensson. “Single-Cell Screening of Photosynthetic Growth and Lactate Production by Cyanobacteria.” Biotechnology for Biofuels. BioMed Central, 2015. https://doi.org/10.1186/s13068-015-0380-2. ieee: P. Hammar et al., “Single-cell screening of photosynthetic growth and lactate production by cyanobacteria,” Biotechnology for Biofuels, vol. 8, no. 1. BioMed Central, 2015. ista: Hammar P, Angermayr A, Sjostrom S, Van Der Meer J, Hellingwerf K, Hudson E, Joensson H. 2015. Single-cell screening of photosynthetic growth and lactate production by cyanobacteria. Biotechnology for Biofuels. 8(1), 193. mla: Hammar, Petter, et al. “Single-Cell Screening of Photosynthetic Growth and Lactate Production by Cyanobacteria.” Biotechnology for Biofuels, vol. 8, no. 1, 193, BioMed Central, 2015, doi:10.1186/s13068-015-0380-2. short: P. Hammar, A. Angermayr, S. Sjostrom, J. Van Der Meer, K. Hellingwerf, E. Hudson, H. Joensson, Biotechnology for Biofuels 8 (2015). date_created: 2018-12-11T11:53:05Z date_published: 2015-11-25T00:00:00Z date_updated: 2021-01-12T06:52:04Z day: '25' ddc: - '570' department: - _id: ToBo doi: 10.1186/s13068-015-0380-2 file: - access_level: open_access checksum: 172b0b6f4eb2e5c22b7cec1d57dc0107 content_type: application/pdf creator: system date_created: 2018-12-12T10:10:11Z date_updated: 2020-07-14T12:45:07Z file_id: '4796' file_name: IST-2016-467-v1+1_s13068-015-0380-2.pdf file_size: 2914089 relation: main_file file_date_updated: 2020-07-14T12:45:07Z has_accepted_license: '1' intvolume: ' 8' issue: '1' language: - iso: eng month: '11' oa: 1 oa_version: Published Version publication: Biotechnology for Biofuels publication_status: published publisher: BioMed Central publist_id: '5537' pubrep_id: '467' quality_controlled: '1' scopus_import: 1 status: public title: Single-cell screening of photosynthetic growth and lactate production by cyanobacteria tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87 volume: 8 year: '2015' ...