{"acknowledged_ssus":[{"_id":"SSU"}],"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5583642/"}],"citation":{"mla":"Kiermaier, Eva, et al. “Polysialylation Controls Dendritic Cell Trafficking by Regulating Chemokine Recognition.” <i>Science</i>, vol. 351, no. 6269, American Association for the Advancement of Science, 2016, pp. 186–90, doi:<a href=\"https://doi.org/10.1126/science.aad0512\">10.1126/science.aad0512</a>.","short":"E. Kiermaier, C. Moussion, C. Veldkamp, R. Gerardy  Schahn, I. de Vries, L. Williams, G. Chaffee, A. Phillips, F. Freiberger, R. Imre, D. Taleski, R. Payne, A. Braun, R. Förster, K. Mechtler, M. Mühlenhoff, B. Volkman, M.K. Sixt, Science 351 (2016) 186–190.","chicago":"Kiermaier, Eva, Christine Moussion, Christopher Veldkamp, Rita Gerardy  Schahn, Ingrid de Vries, Larry Williams, Gary Chaffee, et al. “Polysialylation Controls Dendritic Cell Trafficking by Regulating Chemokine Recognition.” <i>Science</i>. American Association for the Advancement of Science, 2016. <a href=\"https://doi.org/10.1126/science.aad0512\">https://doi.org/10.1126/science.aad0512</a>.","ista":"Kiermaier E, Moussion C, Veldkamp C, Gerardy  Schahn R, de Vries I, Williams L, Chaffee G, Phillips A, Freiberger F, Imre R, Taleski D, Payne R, Braun A, Förster R, Mechtler K, Mühlenhoff M, Volkman B, Sixt MK. 2016. Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. Science. 351(6269), 186–190.","ama":"Kiermaier E, Moussion C, Veldkamp C, et al. Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. <i>Science</i>. 2016;351(6269):186-190. doi:<a href=\"https://doi.org/10.1126/science.aad0512\">10.1126/science.aad0512</a>","ieee":"E. Kiermaier <i>et al.</i>, “Polysialylation controls dendritic cell trafficking by regulating chemokine recognition,” <i>Science</i>, vol. 351, no. 6269. American Association for the Advancement of Science, pp. 186–190, 2016.","apa":"Kiermaier, E., Moussion, C., Veldkamp, C., Gerardy  Schahn, R., de Vries, I., Williams, L., … Sixt, M. K. (2016). Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aad0512\">https://doi.org/10.1126/science.aad0512</a>"},"article_processing_charge":"No","date_updated":"2021-01-12T06:51:52Z","article_type":"original","publication_status":"published","issue":"6269","publication":"Science","month":"01","department":[{"_id":"MiSi"}],"type":"journal_article","intvolume":"       351","title":"Polysialylation controls dendritic cell trafficking by regulating chemokine recognition","abstract":[{"lang":"eng","text":"The addition of polysialic acid to N- and/or O-linked glycans, referred to as polysialylation, is a rare posttranslational modification that is mainly known to control the developmental plasticity of the nervous system. Here we show that CCR7, the central chemokine receptor controlling immune cell trafficking to secondary lymphatic organs, carries polysialic acid. This modification is essential for the recognition of the CCR7 ligand CCL21. As a consequence, dendritic cell trafficking is abrogated in polysialyltransferase-deficient mice, manifesting as disturbed lymph node homeostasis and unresponsiveness to inflammatory stimuli. Structure-function analysis of chemokine-receptor interactions reveals that CCL21 adopts an autoinhibited conformation, which is released upon interaction with polysialic acid. Thus, we describe a glycosylation-mediated immune cell trafficking disorder and its mechanistic basis.\r\n"}],"acknowledgement":"We thank S. Schüchner and E. Ogris for kindly providing the antibody to GFP, M. Helmbrecht and A. Huber for providing Nrp2−/− mice, the IST Scientific Support Facilities for excellent services, and J. Renkawitz and K. Vaahtomeri for critically reading the manuscript. ","doi":"10.1126/science.aad0512","ec_funded":1,"publisher":"American Association for the Advancement of Science","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","grant_number":"281556","call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"name":"Stromal Cell-immune Cell Interactions in Health and Disease","call_identifier":"FP7","_id":"25A76F58-B435-11E9-9278-68D0E5697425","grant_number":"289720"},{"name":"Cytoskeletal force generation and transduction of leukocytes (FWF)","call_identifier":"FWF","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12"}],"status":"public","pmid":1,"oa":1,"day":"08","date_published":"2016-01-08T00:00:00Z","year":"2016","quality_controlled":"1","date_created":"2018-12-11T11:52:57Z","volume":351,"external_id":{"pmid":["26657283"]},"_id":"1599","publist_id":"5570","oa_version":"Submitted Version","scopus_import":1,"author":[{"last_name":"Kiermaier","first_name":"Eva","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","full_name":"Kiermaier, Eva","orcid":"0000-0001-6165-5738"},{"full_name":"Moussion, Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87","first_name":"Christine","last_name":"Moussion"},{"last_name":"Veldkamp","first_name":"Christopher","full_name":"Veldkamp, Christopher"},{"full_name":"Gerardy  Schahn, Rita","last_name":"Gerardy  Schahn","first_name":"Rita"},{"full_name":"De Vries, Ingrid","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries"},{"full_name":"Williams, Larry","last_name":"Williams","first_name":"Larry"},{"full_name":"Chaffee, Gary","first_name":"Gary","last_name":"Chaffee"},{"last_name":"Phillips","first_name":"Andrew","full_name":"Phillips, Andrew"},{"first_name":"Friedrich","last_name":"Freiberger","full_name":"Freiberger, Friedrich"},{"full_name":"Imre, Richard","last_name":"Imre","first_name":"Richard"},{"full_name":"Taleski, Deni","first_name":"Deni","last_name":"Taleski"},{"full_name":"Payne, Richard","last_name":"Payne","first_name":"Richard"},{"last_name":"Braun","first_name":"Asolina","full_name":"Braun, Asolina"},{"full_name":"Förster, Reinhold","first_name":"Reinhold","last_name":"Förster"},{"full_name":"Mechtler, Karl","last_name":"Mechtler","first_name":"Karl"},{"full_name":"Mühlenhoff, Martina","last_name":"Mühlenhoff","first_name":"Martina"},{"full_name":"Volkman, Brian","last_name":"Volkman","first_name":"Brian"},{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"page":"186 - 190","language":[{"iso":"eng"}]}