{"_id":"1383","oa":1,"scopus_import":1,"publication_status":"published","language":[{"iso":"eng"}],"publication":"Nature Plants","date_created":"2018-12-11T11:51:42Z","abstract":[{"lang":"eng","text":"In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants."}],"volume":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","publisher":"Nature Publishing Group","day":"06","department":[{"_id":"JiFr"}],"pmid":1,"doi":"10.1038/nplants.2015.94","type":"journal_article","article_type":"original","issue":"7","article_number":"15094","external_id":{"pmid":["27250258"]},"status":"public","author":[{"last_name":"Yu","first_name":"Luo","full_name":"Yu, Luo"},{"last_name":"Scholl","first_name":"Stefan","full_name":"Scholl, Stefan"},{"first_name":"Anett","last_name":"Doering","full_name":"Doering, Anett"},{"full_name":"Yi, Zhang","first_name":"Zhang","last_name":"Yi"},{"full_name":"Irani, Niloufer","last_name":"Irani","first_name":"Niloufer"},{"full_name":"Di Rubbo, Simone","first_name":"Simone","last_name":"Di Rubbo"},{"full_name":"Neumetzler, Lutz","first_name":"Lutz","last_name":"Neumetzler"},{"full_name":"Krishnamoorthy, Praveen","last_name":"Krishnamoorthy","first_name":"Praveen"},{"last_name":"Van Houtte","first_name":"Isabelle","full_name":"Van Houtte, Isabelle"},{"full_name":"Mylle, Evelien","first_name":"Evelien","last_name":"Mylle"},{"first_name":"Volker","last_name":"Bischoff","full_name":"Bischoff, Volker"},{"first_name":"Samantha","last_name":"Vernhettes","full_name":"Vernhettes, Samantha"},{"first_name":"Johan","last_name":"Winne","full_name":"Winne, Johan"},{"last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"},{"full_name":"Stierhof, York","first_name":"York","last_name":"Stierhof"},{"full_name":"Schumacher, Karin","first_name":"Karin","last_name":"Schumacher"},{"full_name":"Persson, Staffan","last_name":"Persson","first_name":"Staffan"},{"full_name":"Russinova, Eugenia","first_name":"Eugenia","last_name":"Russinova"}],"oa_version":"Submitted Version","publist_id":"5827","date_updated":"2021-01-12T06:50:18Z","month":"07","year":"2015","quality_controlled":"1","title":"V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905525/","open_access":"1"}],"intvolume":"         1","citation":{"ieee":"L. Yu <i>et al.</i>, “V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis,” <i>Nature Plants</i>, vol. 1, no. 7. Nature Publishing Group, 2015.","ama":"Yu L, Scholl S, Doering A, et al. V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. <i>Nature Plants</i>. 2015;1(7). doi:<a href=\"https://doi.org/10.1038/nplants.2015.94\">10.1038/nplants.2015.94</a>","short":"L. Yu, S. Scholl, A. Doering, Z. Yi, N. Irani, S. Di Rubbo, L. Neumetzler, P. Krishnamoorthy, I. Van Houtte, E. Mylle, V. Bischoff, S. Vernhettes, J. Winne, J. Friml, Y. Stierhof, K. Schumacher, S. Persson, E. Russinova, Nature Plants 1 (2015).","mla":"Yu, Luo, et al. “V-ATPase Activity in the TGN/EE Is Required for Exocytosis and Recycling in Arabidopsis.” <i>Nature Plants</i>, vol. 1, no. 7, 15094, Nature Publishing Group, 2015, doi:<a href=\"https://doi.org/10.1038/nplants.2015.94\">10.1038/nplants.2015.94</a>.","ista":"Yu L, Scholl S, Doering A, Yi Z, Irani N, Di Rubbo S, Neumetzler L, Krishnamoorthy P, Van Houtte I, Mylle E, Bischoff V, Vernhettes S, Winne J, Friml J, Stierhof Y, Schumacher K, Persson S, Russinova E. 2015. V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. Nature Plants. 1(7), 15094.","apa":"Yu, L., Scholl, S., Doering, A., Yi, Z., Irani, N., Di Rubbo, S., … Russinova, E. (2015). V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis. <i>Nature Plants</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nplants.2015.94\">https://doi.org/10.1038/nplants.2015.94</a>","chicago":"Yu, Luo, Stefan Scholl, Anett Doering, Zhang Yi, Niloufer Irani, Simone Di Rubbo, Lutz Neumetzler, et al. “V-ATPase Activity in the TGN/EE Is Required for Exocytosis and Recycling in Arabidopsis.” <i>Nature Plants</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/nplants.2015.94\">https://doi.org/10.1038/nplants.2015.94</a>."},"date_published":"2015-07-06T00:00:00Z"}