Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants

M. Eremina, S. Unterholzner, A. Rathnayake, M. Castellanos, M. Khan-Djamei, K. Kügler, S. May, K. Mayer, W. Rozhon, B. Poppenberger, PNAS 113 (2016) E5982–E5991.


Journal Article | Published | English
Author
Eremina, Marina; Unterholzner, Simon; Rathnayake, Ajith; Castellanos, Marcos; Khan-Djamei, MamoonaIST Austria; Kügler, Karl; May, Sean; Mayer, Klaus; Rozhon, Wilfried; Poppenberger, Brigitte
Abstract
Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold acclimation. The constitutive activation of BR signaling, in contrast, enhanced freezing resistance. Evidence is provided that the BR-controlled basic helix-loop-helix transcription factor CESTA (CES) can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR genes are identified that are regulated in a BR- and CES-dependent manner during cold acclimation. A model is presented in which BRs govern different cold-responsive transcriptional cascades through the post-translational modification of CES and redundantly acting factors. This contributes to the basal resistance against freezing stress, but also to the further improvement of this resistance through cold acclimation.
Publishing Year
Date Published
2016-10-04
Journal Title
PNAS
Acknowledgement
We thank Joanne Chory for seeds of the bee1 bee2 bee3, bes1-D, and bzr1-1D mutants and the 35S:BRI1-GFP line; Irene Ziegler, Clarissa Fahrig, and Renata Milcevicova for technical assistance; and the horticultural staff of the TUMs Gewächshauslaborzentrum Dürnast for plant care. This work was supported by funds from the Austrian Science Fund (Project P22734 to B.P.), the Deutsche Forschungsgemeinschaft (Project PO1640/4 to B.P. and SFB924 to B.P. and K.F.X.M.), and a TUM doctoral fellowship (to M.E.). M.E. and S.J.U. were members of the TUM graduate school.
Volume
113
Issue
40
Page
E5982 - E5991
IST-REx-ID

Cite this

Eremina M, Unterholzner S, Rathnayake A, et al. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. 2016;113(40):E5982-E5991. doi:10.1073/pnas.1611477113
Eremina, M., Unterholzner, S., Rathnayake, A., Castellanos, M., Khan-Djamei, M., Kügler, K., … Poppenberger, B. (2016). Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS, 113(40), E5982–E5991. https://doi.org/10.1073/pnas.1611477113
Eremina, Marina, Simon Unterholzner, Ajith Rathnayake, Marcos Castellanos, Mamoona Khan-Djamei, Karl Kügler, Sean May, Klaus Mayer, Wilfried Rozhon, and Brigitte Poppenberger. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” PNAS 113, no. 40 (2016): E5982–91. https://doi.org/10.1073/pnas.1611477113.
M. Eremina et al., “Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants,” PNAS, vol. 113, no. 40, pp. E5982–E5991, 2016.
Eremina M, Unterholzner S, Rathnayake A, Castellanos M, Khan-Djamei M, Kügler K, May S, Mayer K, Rozhon W, Poppenberger B. 2016. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. 113(40), E5982–E5991.
Eremina, Marina, et al. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” PNAS, vol. 113, no. 40, National Academy of Sciences, 2016, pp. E5982–91, doi:10.1073/pnas.1611477113.
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