Structures of multisubunit membrane complexes with the CRYO ARM 200

Gerle C, Kishikawa J, Yamaguchi T, Nakanishi A, Çoruh MO, Makino F, Miyata T, Kawamoto A, Yokoyama K, Namba K, Kurisu G, Kato T. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy.

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Journal Article | Accepted | English
Author
Gerle, Christoph; Kishikawa, Jun-ichi; Yamaguchi, Tomoko; Nakanishi, Atsuko; Çoruh, Mehmet OrkunISTA ; Makino, Fumiaki; Miyata, Tomoko; Kawamoto, Akihiro; Yokoyama, Ken; Namba, Keiichi; Kurisu, Genji; Kato, Takayuki
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Department
Abstract
Progress in structural membrane biology has been significantly accelerated by the ongoing 'Resolution Revolution' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica.
Publishing Year
Date Published
2022-07-21
Journal Title
Microscopy
ISSN
eISSN
IST-REx-ID

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Gerle C, Kishikawa J, Yamaguchi T, et al. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. doi:10.1093/jmicro/dfac037
Gerle, C., Kishikawa, J., Yamaguchi, T., Nakanishi, A., Çoruh, M. O., Makino, F., … Kato, T. (n.d.). Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. Oxford University Press. https://doi.org/10.1093/jmicro/dfac037
Gerle, Christoph, Jun-ichi Kishikawa, Tomoko Yamaguchi, Atsuko Nakanishi, Mehmet Orkun Çoruh, Fumiaki Makino, Tomoko Miyata, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” Microscopy. Oxford University Press, n.d. https://doi.org/10.1093/jmicro/dfac037.
C. Gerle et al., “Structures of multisubunit membrane complexes with the CRYO ARM 200,” Microscopy. Oxford University Press.
Gerle C, Kishikawa J, Yamaguchi T, Nakanishi A, Çoruh MO, Makino F, Miyata T, Kawamoto A, Yokoyama K, Namba K, Kurisu G, Kato T. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy.
Gerle, Christoph, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” Microscopy, Oxford University Press, doi:10.1093/jmicro/dfac037.

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PMID: 35861182
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