--- res: bibo_abstract: - "Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue. \r\n@eng" bibo_authorlist: - foaf_Person: foaf_givenName: Jan foaf_name: Schwarz, Jan foaf_surname: Schwarz foaf_workInfoHomepage: http://www.librecat.org/personId=346C1EC6-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Veronika foaf_name: Bierbaum, Veronika foaf_surname: Bierbaum foaf_workInfoHomepage: http://www.librecat.org/personId=3FD04378-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Jack foaf_name: Merrin, Jack foaf_surname: Merrin foaf_workInfoHomepage: http://www.librecat.org/personId=4515C308-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-5145-4609 - foaf_Person: foaf_givenName: Tino foaf_name: Frank, Tino foaf_surname: Frank - foaf_Person: foaf_givenName: Robert foaf_name: Hauschild, Robert foaf_surname: Hauschild foaf_workInfoHomepage: http://www.librecat.org/personId=4E01D6B4-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-9843-3522 - foaf_Person: foaf_givenName: Mark Tobias foaf_name: Bollenbach, Mark Tobias foaf_surname: Bollenbach foaf_workInfoHomepage: http://www.librecat.org/personId=3E6DB97A-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-4398-476X - foaf_Person: foaf_givenName: Savaş foaf_name: Tay, Savaş foaf_surname: Tay - foaf_Person: foaf_givenName: Michael K foaf_name: Sixt, Michael K foaf_surname: Sixt foaf_workInfoHomepage: http://www.librecat.org/personId=41E9FBEA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6620-9179 - foaf_Person: foaf_givenName: Matthias foaf_name: Mehling, Matthias foaf_surname: Mehling foaf_workInfoHomepage: http://www.librecat.org/personId=3C23B994-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8599-1226 bibo_doi: 10.1038/srep36440 bibo_volume: 6 dct_date: 2016^xs_gYear dct_language: eng dct_publisher: Nature Publishing Group@ dct_title: A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients@ ...