---
_id: '11489'
abstract:
- lang: eng
text: Much of plant development depends on cell-to-cell redistribution of the plant
hormone auxin, which is facilitated by the plasma membrane (PM) localized PIN
FORMED (PIN) proteins. Auxin export activity, developmental roles, subcellular
trafficking, and polarity of PINs have been well studied, but their structure
remains elusive besides a rough outline that they contain two groups of 5 alpha-helices
connected by a large hydrophilic loop (HL). Here, we focus on the PIN1 HL as we
could produce it in sufficient quantities for biochemical investigations to provide
insights into its secondary structure. Circular dichroism (CD) studies revealed
its nature as an intrinsically disordered protein (IDP), manifested by the increase
of structure content upon thermal melting. Consistent with IDPs serving as interaction
platforms, PIN1 loops homodimerize. PIN1 HL cytoplasmic overexpression in Arabidopsis
disrupts early endocytic trafficking of PIN1 and PIN2 and causes defects in the
cotyledon vasculature formation. In summary, we demonstrate that PIN1 HL has an
intrinsically disordered nature, which must be considered to gain further structural
insights. Some secondary structures may form transiently during pairing with known
and yet-to-be-discovered interactors.
acknowledgement: 'We thank Charo del Genio from Coventry University and Richard Napier
from the University of Warwick for helpful discussion concerning protein modeling
and inspiration concerning CD spectroscopy, respectively. We thank Jan Hejatko for
sharing the published AHP2 construct. We also thank Josef Houser from the core facility
BIC CEITEC for valuable assistance, discussions, and ideas relating to CD. We acknowledge
the: Core Facility CELLIM of CEITEC supported by the Czech-BioImaging large RI project
(LM2018129 funded by MEYS CR), part of the Euro-BioImaging (www.eurobioimaging.eu
accessed on 1 January 2016) ALM and medical imaging Node (Brno, CZ), CF Biomolecular
Interactions and Crystallization of CIISB, Instruct-CZ Centre, supported by MEYS
CR (LM2018127) and European Regional Development Fund-Project “UP CIISB“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974)
for their support with obtaining scientific data presented in this paper; Plant
Sciences Core Facility of CEITEC Masaryk University for technical support. Open
Access Funding by the Austrian Science Fund (FWF).'
article_processing_charge: Yes
article_type: original
author:
- first_name: V
full_name: Bilanovičová, V
last_name: Bilanovičová
- first_name: N
full_name: Rýdza, N
last_name: Rýdza
- first_name: L
full_name: Koczka, L
last_name: Koczka
- first_name: M
full_name: Hess, M
last_name: Hess
- first_name: E
full_name: Feraru, E
last_name: Feraru
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: T
full_name: Nodzyński, T
last_name: Nodzyński
citation:
ama: Bilanovičová V, Rýdza N, Koczka L, et al. The hydrophilic loop of Arabidopsis
PIN1 auxin efflux carrier harbors hallmarks of an intrinsically disordered protein.
International Journal of Molecular Sciences. 2022;23(11):6352. doi:10.3390/ijms23116352
apa: Bilanovičová, V., Rýdza, N., Koczka, L., Hess, M., Feraru, E., Friml, J., &
Nodzyński, T. (2022). The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier
harbors hallmarks of an intrinsically disordered protein. International Journal
of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms23116352
chicago: Bilanovičová, V, N Rýdza, L Koczka, M Hess, E Feraru, Jiří Friml, and T
Nodzyński. “The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux Carrier Harbors
Hallmarks of an Intrinsically Disordered Protein.” International Journal of
Molecular Sciences. MDPI, 2022. https://doi.org/10.3390/ijms23116352.
ieee: V. Bilanovičová et al., “The hydrophilic loop of Arabidopsis PIN1 auxin
efflux carrier harbors hallmarks of an intrinsically disordered protein,” International
Journal of Molecular Sciences, vol. 23, no. 11. MDPI, p. 6352, 2022.
ista: Bilanovičová V, Rýdza N, Koczka L, Hess M, Feraru E, Friml J, Nodzyński T.
2022. The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks
of an intrinsically disordered protein. International Journal of Molecular Sciences.
23(11), 6352.
mla: Bilanovičová, V., et al. “The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux
Carrier Harbors Hallmarks of an Intrinsically Disordered Protein.” International
Journal of Molecular Sciences, vol. 23, no. 11, MDPI, 2022, p. 6352, doi:10.3390/ijms23116352.
short: V. Bilanovičová, N. Rýdza, L. Koczka, M. Hess, E. Feraru, J. Friml, T. Nodzyński,
International Journal of Molecular Sciences 23 (2022) 6352.
date_created: 2022-07-05T15:14:34Z
date_published: 2022-06-06T00:00:00Z
date_updated: 2023-08-09T10:13:57Z
day: '06'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.3390/ijms23116352
external_id:
isi:
- '000808733300001'
pmid:
- '35683031'
file:
- access_level: open_access
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creator: cchlebak
date_created: 2022-07-06T07:36:59Z
date_updated: 2022-07-06T07:36:59Z
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relation: main_file
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intvolume: ' 23'
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language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '06'
oa: 1
oa_version: Published Version
page: '6352'
pmid: 1
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29988
name: RNA-directed DNA methylation in plant development
publication: International Journal of Molecular Sciences
publication_identifier:
issn:
- 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
status: public
title: The hydrophilic loop of Arabidopsis PIN1 auxin efflux carrier harbors hallmarks
of an intrinsically disordered protein
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2022'
...