Microtubule aging probed by microfluidics assisted tubulin washout

C.F. Düllberg, N. Cade, T. Surrey, Molecular Biology and Evolution 27 (2016) 3563–3573.

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Journal Article | Published | English
Author
Düllberg, Christian FIST Austria; Cade, Nicholas; Surrey, Thomas
Abstract
Microtubules switch stochastically between phases of growth and shrinkage. The molecular mechanism responsible for the end of a growth phase, an event called catastrophe, is still not understood. The probability for a catastrophe to occur increases with microtubule age, putting constraints on the possible molecular mechanism of catastrophe induction. Here we used microfluidics-Assisted fast tubulin washout experiments to induce microtubule depolymerization in a controlled manner at different times after the start of growth. We found that aging can also be observed in this assay, providing valuable new constraints against which theoretical models of catastrophe induction can be tested. We found that the data can be quantitatively well explained by a simple kinetic threshold model that assumes an age-dependent broadening of the protective cap at the microtubule end as a result of an evolving tapered end structure; this leads to a decrease of the cap density and its stability. This analysis suggests an intuitive picture of the role of morphological changes of the protective cap for the age dependence of microtubule stability.
Publishing Year
Date Published
2016-11-07
Journal Title
Molecular Biology and Evolution
Volume
27
Issue
22
Page
3563 - 3573
IST-REx-ID

Cite this

Düllberg CF, Cade N, Surrey T. Microtubule aging probed by microfluidics assisted tubulin washout. Molecular Biology and Evolution. 2016;27(22):3563-3573. doi:10.1091/mbc.E16-07-0548
Düllberg, C. F., Cade, N., & Surrey, T. (2016). Microtubule aging probed by microfluidics assisted tubulin washout. Molecular Biology and Evolution, 27(22), 3563–3573. https://doi.org/10.1091/mbc.E16-07-0548
Düllberg, Christian F, Nicholas Cade, and Thomas Surrey. “Microtubule Aging Probed by Microfluidics Assisted Tubulin Washout.” Molecular Biology and Evolution 27, no. 22 (2016): 3563–73. https://doi.org/10.1091/mbc.E16-07-0548.
C. F. Düllberg, N. Cade, and T. Surrey, “Microtubule aging probed by microfluidics assisted tubulin washout,” Molecular Biology and Evolution, vol. 27, no. 22, pp. 3563–3573, 2016.
Düllberg CF, Cade N, Surrey T. 2016. Microtubule aging probed by microfluidics assisted tubulin washout. Molecular Biology and Evolution. 27(22), 3563–3573.
Düllberg, Christian F., et al. “Microtubule Aging Probed by Microfluidics Assisted Tubulin Washout.” Molecular Biology and Evolution, vol. 27, no. 22, Oxford University Press, 2016, pp. 3563–73, doi:10.1091/mbc.E16-07-0548.

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