--- res: bibo_abstract: - Complex I is one of the major respiratory complexes, conserved from bacteria to mammals. It oxidises NADH, reduces quinone and pumps protons across the membrane, thus playing a central role in the oxidative energy metabolism. In this review we discuss our current state of understanding the structure of complex I from various species of mammals, plants, fungi, and bacteria, as well as of several complex I-related proteins. By comparing the structural evidence from these systems in different redox states and data from mutagenesis and molecular simulations, we formulate the mechanisms of electron transfer and proton pumping and explain how they are conformationally and electrostatically coupled. Finally, we discuss the structural basis of the deactivation phenomenon in mammalian complex I.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Domen foaf_name: Kampjut, Domen foaf_surname: Kampjut foaf_workInfoHomepage: http://www.librecat.org/personId=37233050-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Leonid A foaf_name: Sazanov, Leonid A foaf_surname: Sazanov foaf_workInfoHomepage: http://www.librecat.org/personId=338D39FE-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0977-7989 bibo_doi: 10.1016/j.sbi.2022.102350 bibo_volume: 74 dct_date: 2022^xs_gYear dct_identifier: - UT:000829029500020 dct_isPartOf: - http://id.crossref.org/issn/0959-440X dct_language: eng dct_publisher: Elsevier@ dct_subject: - Molecular Biology - Structural Biology dct_title: Structure of respiratory complex I – An emerging blueprint for the mechanism@ fabio_hasPubmedId: '35316665' ...