Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots

Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S. 2022. Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany., erac019.

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Journal Article | Epub ahead of print | English
Author
Wang, R; Himschoot, E; Grenzi, M; Chen, J; Safi, A; Krebs, M; Schumacher, K; Nowack, MK; Moeder, W; Yoshioka, K; Van Damme, D; De Smet, I
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Abstract
Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven to be instrumental to reveal the various faces of auxin, they display in some cases distinct bioactivities compared to IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive endosomal aggregations (BFA bodies), and the correlation with the ability to elicit Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.
Publishing Year
Date Published
2022-01-27
Journal Title
Journal of Experimental Botany
Article Number
erac019
ISSN
eISSN
IST-REx-ID

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Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany. 2022. doi:10.1093/jxb/erac019
Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste, S. (2022). Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/erac019
Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher, et al. “Auxin Analog-Induced Ca 2+ Signaling Is Not Involved in Inhibition of Endosomal Aggregation in Arabidopsis Roots.” Journal of Experimental Botany. Oxford University Press, 2022. https://doi.org/10.1093/jxb/erac019.
R. Wang et al., “Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots,” Journal of Experimental Botany. Oxford University Press, 2022.
Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S. 2022. Auxin analog-induced Ca 2+ signaling is not involved in inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany., erac019.
Wang, R., et al. “Auxin Analog-Induced Ca 2+ Signaling Is Not Involved in Inhibition of Endosomal Aggregation in Arabidopsis Roots.” Journal of Experimental Botany, erac019, Oxford University Press, 2022, doi:10.1093/jxb/erac019.

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PMID: 35085386
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