--- res: bibo_abstract: - Growth regulation tailors development in plants to their environment. A prominent example of this is the response to gravity, in which shoots bend up and roots bend down1. This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots while inhibiting it in roots via a yet unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding of how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on rapid regulation of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization. Simultaneous activation of these two counteracting mechanisms poises roots for rapid, fine-tuned growth modulation in navigating complex soil environments.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Lanxin foaf_name: Li, Lanxin foaf_surname: Li foaf_workInfoHomepage: http://www.librecat.org/personId=367EF8FA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-5607-272X - foaf_Person: foaf_givenName: Inge foaf_name: Verstraeten, Inge foaf_surname: Verstraeten foaf_workInfoHomepage: http://www.librecat.org/personId=362BF7FE-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-7241-2328 - foaf_Person: foaf_givenName: Mark foaf_name: Roosjen, Mark foaf_surname: Roosjen - foaf_Person: foaf_givenName: Koji foaf_name: Takahashi, Koji foaf_surname: Takahashi - foaf_Person: foaf_givenName: Lesia foaf_name: Rodriguez Solovey, Lesia foaf_surname: Rodriguez Solovey foaf_workInfoHomepage: http://www.librecat.org/personId=3922B506-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-7244-7237 - foaf_Person: foaf_givenName: Jack foaf_name: Merrin, Jack foaf_surname: Merrin foaf_workInfoHomepage: http://www.librecat.org/personId=4515C308-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-5145-4609 - foaf_Person: foaf_givenName: Jian foaf_name: Chen, Jian foaf_surname: Chen - foaf_Person: foaf_givenName: Lana foaf_name: Shabala, Lana foaf_surname: Shabala - foaf_Person: foaf_givenName: Wouter foaf_name: Smet, Wouter foaf_surname: Smet - foaf_Person: foaf_givenName: Hong foaf_name: Ren, Hong foaf_surname: Ren - foaf_Person: foaf_givenName: Steffen foaf_name: Vanneste, Steffen foaf_surname: Vanneste - foaf_Person: foaf_givenName: Sergey foaf_name: Shabala, Sergey foaf_surname: Shabala - foaf_Person: foaf_givenName: Bert foaf_name: De Rybel, Bert foaf_surname: De Rybel - foaf_Person: foaf_givenName: Dolf foaf_name: Weijers, Dolf foaf_surname: Weijers - foaf_Person: foaf_givenName: Toshinori foaf_name: Kinoshita, Toshinori foaf_surname: Kinoshita - foaf_Person: foaf_givenName: William M. foaf_name: Gray, William M. foaf_surname: Gray - foaf_Person: foaf_givenName: Jiří foaf_name: Friml, Jiří foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1038/s41586-021-04037-6 bibo_issue: '7884' bibo_volume: 599 dct_date: 2021^xs_gYear dct_identifier: - UT:000713338100006 dct_isPartOf: - http://id.crossref.org/issn/00280836 - http://id.crossref.org/issn/14764687 dct_language: eng dct_publisher: Springer Nature@ dct_subject: - Multidisciplinary dct_title: Cell surface and intracellular auxin signalling for H+ fluxes in root growth@ fabio_hasPubmedId: '34707283' ...