Polarity and subcellular dynamics in plants

Project Period: 2013-04-01 – 2017-01-31
Externally Funded
Acronym
PSDP
Principal Investigator
Jirí Friml
Department(s)
Friml Group
Description
Plant life strategy is marked by acquisition of highly flexible development that adapts plants’ phenotype to the environment. Various environmental signals are integrated into the endogenous signalling networks involving the versatile phytohormone auxin. The intercellular auxin transport mediates a large variety of adaptive plant growth responses. Subcellular polar distribution of PIN auxin transporters determines directionality of auxin flow and thus have potential to integrate internal and external signals via the redirection of auxin fluxes and translate them into modulation of development. Auxin transport thus represents a unique model for studying the functional link between basic cellular processes, such as vesicle trafficking and cell polarity, and their developmental outcome at the level of the multicellular organism. We will employ approaches of cell biology, molecular genetics and chemical genomics in Arabidopsis thaliana to identify the cellular and molecular mechanisms regulating the directional throughput of auxin flow a integration of environmental signals into subcellular dynamics of PIN auxin transporters as well as endogenous feed-back regulations of this mechanism. In our proposal, we will focus on four main research directions. 1. Novel regulators of cell polarity identified by chemical genomics 2. Cellular mechanisms of cell polarity maintenance 3. Integration of signals into subcellular dynamics of auxin transport 4. Mathematical modelling of regulatory circuits for adaptive development The results will demonstrate the viability of genetics approaches for addressing cell biological questions in plants, open new horizons in plant cell biology and plant hormone fields and inspire researchers also in non-plant fields. The expected output has clear application potential for targeted modulation of plant development. The project will further strengthen our position of world-leading laboratory in plant hormone and plant cell biology fields.
Grant Number
282300
Funding Organisation
FP7_ERC

40 Publications

2015 | Journal Article | IST-REx-ID: 1819
Osmotic stress modulates the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis thaliana
M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, J. Friml, Molecular Plant 8 (2015) 1175–1187.
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2014 | Journal Article | IST-REx-ID: 2223   OA
BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis
H. Tanaka, T. Nodzyński, S. Kitakura, M. Feraru, M. Sasabe, T. Ishikawa, J. Kleine Vehn, T. Kakimoto, J. Friml, Plant and Cell Physiology 55 (2014) 737–749.
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2018 | Journal Article | IST-REx-ID: 428   OA
Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane
Y. Salanenka, I. Verstraeten, C. Löfke, K. Tabata, S. Naramoto, M. Glanc, J. Friml, PNAS 115 (2018) 3716–3721.
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2015 | Journal Article | IST-REx-ID: 1509   OA
Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
J. Michalko, M. Dravecka, T. Bollenbach, J. Friml, F1000 Research 4 (2015).
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2017 | Journal Article | IST-REx-ID: 1078
Light sheet fluorescence microscopy of plant roots growing on the surface of a gel
D. Von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments JoVE 2017 (2017) e55044.
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2016 | Journal Article | IST-REx-ID: 1145   OA
Enquiry into the topology of plasma membrane localized PIN auxin transport components
T. Nodzyński, S. Vanneste, M. Zwiewka, M. Pernisová, J. Hejátko, J. Friml, Molecular Plant 9 (2016) 1504–1519.
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2018 | Journal Article | IST-REx-ID: 191   OA
PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism
P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J. Friml, Scientific Reports 8 (2018).
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2017 | Book Chapter | IST-REx-ID: 545   OA
Vascular tissue development and regeneration in the model plant arabidopsis
E. Mazur, J. Friml, in:, S. Jurić (Ed.), Plant Engineering, InTech, 2017, pp. 113–140.
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2018 | Journal Article | IST-REx-ID: 913   OA
PATELLINS are regulators of auxin mediated PIN1 relocation and plant development in Arabidopsis thaliana
R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, J. Friml, Journal of Cell Science 131 (2018) jcs.204198.
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2018 | Journal Article | IST-REx-ID: 449   OA
WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity
T. Prat, J. Hajny, W. Grunewald, M.K. Vasileva, G. Molnar, R. Tejos, M. Schmid, M. Sauer, J. Friml, PLoS Genetics 14 (2018).
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2018 | Journal Article | IST-REx-ID: 158   OA
Maternal auxin supply contributes to early embryo patterning in Arabidopsis
H. Robert, C. Park, C. Gutièrrez, B. Wójcikowska, A. Pěnčík, O. Novák, J. Chen, W. Grunewald, T. Dresselhaus, J. Friml, T. Laux, Nature Plants 4 (2018) 548–553.
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2016 | Journal Article | IST-REx-ID: 1081   OA
Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells
Ł. Łangowski, K.T. Wabnik, H. Li, S. Vanneste, S. Naramoto, H. Tanaka, J. Friml, Cell Discovery 2 (2016).
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2016 | Journal Article | IST-REx-ID: 1264   OA
Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier
G. Sancho Andrés, E. Soriano Ortega, C. Gao, J. Bernabé Orts, M. Narasimhan, A. Müller, R. Tejos, L. Jiang, J. Friml, F. Aniento, M. Marcote, Plant Physiology 171 (2016) 1965–1982.
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2016 | Journal Article | IST-REx-ID: 1221   OA View | Files available | DOI
 
2016 | Journal Article | IST-REx-ID: 1346   OA
Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification
W. Dejonghe, S. Kuenen, E. Mylle, M.K. Vasileva, O. Keech, C. Viotti, J. Swerts, M. Fendrych, F. Ortiz Morea, K. Mishev, S. Delang, S. Scholl, X. Zarza, M. Heilmann, J. Kourelis, J. Kasprowicz, L. Nguyen, A. Drozdzecki, I. Van Houtte, A. Szatmári, M. Majda, G. Baisa, S. Bednarek, S. Robert, D. Audenaert, C. Testerink, T. Munnik, D. Van Damme, I. Heilmann, K. Schumacher, J. Winne, J. Friml, P. Verstreken, E. Russinova, Nature Communications 7 (2016).
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2015 | Journal Article | IST-REx-ID: 1562
Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles
P. Grones, X. Chen, S. Simon, W. Kaufmann, R. De Rycke, T. Nodzyński, E. Zažímalová, J. Friml, Journal of Experimental Botany 66 (2015) 5055–5065.
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2015 | Journal Article | IST-REx-ID: 1865
Plant embryogenesis requires AUX/LAX-mediated auxin influx
H. Robert, W. Grunewald, M. Sauer, B. Cannoot, M. Soriano, R. Swarup, D. Weijers, M. Bennett, K. Boutilier, J. Friml, Development 142 (2015) 702–711.
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2018 | Journal Article | IST-REx-ID: 203   OA
Auxin methylation is required for differential growth in Arabidopsis
M. Abbas, G.J. Hernández, S. Pollmann, S.L. Samodelov, M. Kolb, J. Friml, U.Z. Hammes, M.D. Zurbriggen, M. Blázquez, D. Alabadí, PNAS 115 (2018) 6864–6869.
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2013 | Journal Article | IST-REx-ID: 2832   OA
Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana
H. Tanaka, S. Kitakura, H. Rakusová, T. Uemura, M. Feraru, R. De Rycke, S. Robert, T. Kakimoto, J. Friml, PLoS Genetics 9 (2013).
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2018 | Journal Article | IST-REx-ID: 412   OA
A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis
M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml, The Plant Cell 30 (2018) 700–716.
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2017 | Journal Article | IST-REx-ID: 1110   OA
Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity
B. Kuhn, T. Nodzyński, S. Errafi, R. Bucher, S. Gupta, B. Aryal, P. Dobrev, L. Bigler, M. Geisler, E. Zažímalová, J. Friml, C. Ringli, Scientific Reports 7 (2017).
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2016 | Journal Article | IST-REx-ID: 1247   OA
ROTUNDA3 function in plant development by phosphatase 2A-mediated regulation of auxin transporter recycling
M. Karampelias, P. Neyt, S. De Groeve, S. Aesaert, G. Coussens, J. Rolčík, L. Bruno, N. De Winne, A. Van Minnebruggen, M. Van Montagu, M. Ponce, J. Micol, J. Friml, G. De Jaeger, M. Van Lijsebettens, PNAS 113 (2016) 2768–2773.
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2018 | Journal Article | IST-REx-ID: 147   OA
The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes
U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois, J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant Cell 30 (2018) 2553–2572.
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2015 | Journal Article | IST-REx-ID: 1569   OA
An early secretory pathway mediated by gnom-like 1 and gnom is essential for basal polarity establishment in Arabidopsis thaliana
S. Doyle, A. Haegera, T. Vain, A. Rigala, C. Viotti, M. Łangowskaa, Q. Maa, J. Friml, N. Raikhel, G. Hickse, S. Robert, PNAS 112 (2015) E806–E815.
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2014 | Journal Article | IST-REx-ID: 1862   OA
Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules
X. Chen, L. Grandont, H. Li, R. Hauschild, S. Paque, A. Abuzeineh, H. Rakusová, E. Benková, C. Perrot Rechenmann, J. Friml, Nature 516 (2014) 90–93.
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2014 | Journal Article | IST-REx-ID: 1994
Directional auxin transport mechanisms in early diverging land plants
T. Viaene, K. Landberg, M. Thelander, E. Medvecka, E. Pederson, E. Feraru, E. Cooper, M. Karimi, C. Delwiche, K. Ljung, M. Geisler, E. Sundberg, J. Friml, Current Biology 24 (2014) 2786–2791.
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2013 | Journal Article | IST-REx-ID: 527
Modeling framework for the establishment of the apical-basal embryonic axis in plants
K.T. Wabnik, H. Robert, R. Smith, J. Friml, Current Biology 23 (2013) 2513–2518.
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2014 | Journal Article | IST-REx-ID: 1893   OA
SAC phosphoinositide phosphatases at the tonoplast mediate vacuolar function in Arabidopsis
P. Nováková, S. Hirsch, E. Feraru, R. Tejos, R. Van Wijk, T. Viaene, M. Heilmann, J. Lerche, R. De Rycke, M. Feraru, P. Grones, M. Van Montagu, I. Heilmann, T. Munnik, J. Friml, PNAS 111 (2014) 2818–2823.
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2015 | Journal Article | IST-REx-ID: 1944
Intracellular trafficking and PIN-mediated cell polarity during tropic responses in plants
H. Rakusová, M. Fendrych, J. Friml, Current Opinion in Plant Biology 23 (2015) 116–123.
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2019 | Journal Article | IST-REx-ID: 6627   OA View | Files available | DOI | PubMed | Europe PMC
 
2016 | Journal Article | IST-REx-ID: 1212   OA
Termination of shoot gravitropic responses by auxin feedback on PIN3 polarity
H. Rakusová, M. Abbas, H. Han, S. Song, H. Robert, J. Friml, Current Biology 26 (2016) 3026–3032.
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2017 | Journal Article | IST-REx-ID: 1159
Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral root formation
W. Steenackers, P. Klíma, M. Quareshy, I. Cesarino, R. Kumpf, S. Corneillie, P. Araújo, T. Viaene, G. Goeminne, M. Nowack, K. Ljung, J. Friml, J. Blakeslee, O. Novák, E. Zažímalová, R. Napier, W. Boerjan, B. Vanholme, Plant Physiology 173 (2017) 552–565.
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2016 | Journal Article | IST-REx-ID: 1344   OA View | Files available | DOI
 
2015 | Journal Article | IST-REx-ID: 1534   OA
Transcriptional regulation of PIN genes by FOUR LIPS and MYB88 during Arabidopsis root gravitropism
H. Wang, K. Yang, J. Zou, L. Zhu, Z. Xie, M. Morita, M. Tasaka, J. Friml, E. Grotewold, T. Beeckman, S. Vanneste, F. Sack, J. Le, Nature Communications 6 (2015).
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2014 | Journal Article | IST-REx-ID: 1921   OA
Bipolar plasma membrane distribution of phosphoinositides and their requirement for auxin-mediated cell polarity and patterning in Arabidopsis
R. Tejos, M. Sauer, S. Vanneste, M. Palacios-Gomez, H. Li, M. Heilmann, R. Van Wijk, J. Vermeer, I. Heilmann, T. Munnik, J. Friml, Plant Cell 26 (2014) 2114–2128.
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2014 | Journal Article | IST-REx-ID: 2222
VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis
S. Naramoto, T. Nodzyński, T. Dainobu, H. Takatsuka, T. Okada, J. Friml, H. Fukuda, Plant and Cell Physiology 55 (2014) 750–763.
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2013 | Journal Article | IST-REx-ID: 2448   OA View | DOI | Download (ext.) | PubMed | Europe PMC
 
2013 | Journal Article | IST-REx-ID: 528
Local auxin sources orient the apical basal axis in arabidopsis embryos
H. Robert, P. Grones, A. Stepanova, L. Robles, A. Lokerse, J. Alonso, D. Weijers, J. Friml, Current Biology 23 (2013) 2506–2512.
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2019 | Journal Article | IST-REx-ID: 6262   OA
Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls
H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal (2019).
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2017 | Journal Article | IST-REx-ID: 946   OA
Live tracking of moving samples in confocal microscopy for vertically grown roots
D. Von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017) e26792.
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