--- _id: '1255' abstract: - lang: eng text: Down syndrome cell adhesion molecule 1 (Dscam1) has widereaching and vital neuronal functions although the role it plays in insect and crustacean immunity is less well understood. In this study, we combine different approaches to understand the roles that Dscam1 plays in fitness-related contexts in two model insect species. Contrary to our expectations, we found no short-term modulation of Dscam1 gene expression after haemocoelic or oral bacterial exposure in Tribolium castaneum, or after haemocoelic bacterial exposure in Drosophila melanogaster. Furthermore, RNAi-mediated Dscam1 knockdown and subsequent bacterial exposure did not reduce T. castaneum survival. However, Dscam1 knockdown in larvae resulted in adult locomotion defects, as well as dramatically reduced fecundity in males and females. We suggest that Dscam1 does not always play a straightforward role in immunity, but strongly influences behaviour and fecundity. This study takes a step towards understanding more about the role of this intriguing gene from different phenotypic perspectives. acknowledgement: "We thank Dietmar Schmucker for reading a draft of this manuscript and thank him and his group for\r\nhelpful discussions. We thank Barbara Hasert, Kevin Ferro and Manuel F. Talarico for technical support and helpful\r\ndiscussions. We also thank two anonymous reviewers for their comments. This study was supported by grants from the Volkswagen Stiftung (1/83 516 and AZ 86020: both to S.A.O.A.) and from the DFG priority programme 1399 ‘Host parasite coevolution’ (KU 1929/4-2 to R.P. and J.K.)." article_number: '160138' author: - first_name: Robert full_name: Peuß, Robert last_name: Peuß - first_name: Kristina full_name: Wensing, Kristina last_name: Wensing - first_name: Luisa full_name: Woestmann, Luisa last_name: Woestmann - first_name: Hendrik full_name: Eggert, Hendrik last_name: Eggert - first_name: Barbara full_name: Milutinovic, Barbara id: 2CDC32B8-F248-11E8-B48F-1D18A9856A87 last_name: Milutinovic orcid: 0000-0002-8214-4758 - first_name: Marlene full_name: Sroka, Marlene last_name: Sroka - first_name: Jörn full_name: Scharsack, Jörn last_name: Scharsack - first_name: Joachim full_name: Kurtz, Joachim last_name: Kurtz - first_name: Sophie full_name: Armitage, Sophie last_name: Armitage citation: ama: 'Peuß R, Wensing K, Woestmann L, et al. Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction. Royal Society Open Science. 2016;3(4). doi:10.1098/rsos.160138' apa: 'Peuß, R., Wensing, K., Woestmann, L., Eggert, H., Milutinovic, B., Sroka, M., … Armitage, S. (2016). Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction. Royal Society Open Science. Royal Society, The. https://doi.org/10.1098/rsos.160138' chicago: 'Peuß, Robert, Kristina Wensing, Luisa Woestmann, Hendrik Eggert, Barbara Milutinovic, Marlene Sroka, Jörn Scharsack, Joachim Kurtz, and Sophie Armitage. “Down Syndrome Cell Adhesion Molecule 1: Testing for a Role in Insect Immunity, Behaviour and Reproduction.” Royal Society Open Science. Royal Society, The, 2016. https://doi.org/10.1098/rsos.160138.' ieee: 'R. Peuß et al., “Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction,” Royal Society Open Science, vol. 3, no. 4. Royal Society, The, 2016.' ista: 'Peuß R, Wensing K, Woestmann L, Eggert H, Milutinovic B, Sroka M, Scharsack J, Kurtz J, Armitage S. 2016. Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction. Royal Society Open Science. 3(4), 160138.' mla: 'Peuß, Robert, et al. “Down Syndrome Cell Adhesion Molecule 1: Testing for a Role in Insect Immunity, Behaviour and Reproduction.” Royal Society Open Science, vol. 3, no. 4, 160138, Royal Society, The, 2016, doi:10.1098/rsos.160138.' short: R. Peuß, K. Wensing, L. Woestmann, H. Eggert, B. Milutinovic, M. Sroka, J. Scharsack, J. Kurtz, S. Armitage, Royal Society Open Science 3 (2016). date_created: 2018-12-11T11:50:58Z date_published: 2016-04-01T00:00:00Z date_updated: 2021-01-12T06:49:25Z day: '01' ddc: - '576' - '592' department: - _id: SyCr doi: 10.1098/rsos.160138 file: - access_level: open_access checksum: c3cd84666c8dc0ce6a784f1c82c1cf68 content_type: application/pdf creator: system date_created: 2018-12-12T10:14:01Z date_updated: 2020-07-14T12:44:41Z file_id: '5049' file_name: IST-2016-704-v1+1_160138.full.pdf file_size: 627377 relation: main_file file_date_updated: 2020-07-14T12:44:41Z has_accepted_license: '1' intvolume: ' 3' issue: '4' language: - iso: eng license: https://creativecommons.org/licenses/by/4.0/ month: '04' oa: 1 oa_version: Published Version publication: Royal Society Open Science publication_status: published publisher: Royal Society, The publist_id: '6070' pubrep_id: '704' quality_controlled: '1' scopus_import: 1 status: public title: 'Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction' tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87 volume: 3 year: '2016' ... --- _id: '1268' acknowledgement: We would like to thank Mihai Netea for inviting us to contribute to this Theme Issue. author: - first_name: Barbara full_name: Milutinovic, Barbara id: 2CDC32B8-F248-11E8-B48F-1D18A9856A87 last_name: Milutinovic orcid: 0000-0002-8214-4758 - first_name: Joachim full_name: Kurtz, Joachim last_name: Kurtz citation: ama: Milutinovic B, Kurtz J. Immune memory in invertebrates. Seminars in Immunology. 2016;28(4):328-342. doi:10.1016/j.smim.2016.05.004 apa: Milutinovic, B., & Kurtz, J. (2016). Immune memory in invertebrates. Seminars in Immunology. Academic Press. https://doi.org/10.1016/j.smim.2016.05.004 chicago: Milutinovic, Barbara, and Joachim Kurtz. “Immune Memory in Invertebrates.” Seminars in Immunology. Academic Press, 2016. https://doi.org/10.1016/j.smim.2016.05.004. ieee: B. Milutinovic and J. Kurtz, “Immune memory in invertebrates,” Seminars in Immunology, vol. 28, no. 4. Academic Press, pp. 328–342, 2016. ista: Milutinovic B, Kurtz J. 2016. Immune memory in invertebrates. Seminars in Immunology. 28(4), 328–342. mla: Milutinovic, Barbara, and Joachim Kurtz. “Immune Memory in Invertebrates.” Seminars in Immunology, vol. 28, no. 4, Academic Press, 2016, pp. 328–42, doi:10.1016/j.smim.2016.05.004. short: B. Milutinovic, J. Kurtz, Seminars in Immunology 28 (2016) 328–342. date_created: 2018-12-11T11:51:03Z date_published: 2016-08-01T00:00:00Z date_updated: 2021-01-12T06:49:30Z day: '01' department: - _id: SyCr doi: 10.1016/j.smim.2016.05.004 intvolume: ' 28' issue: '4' language: - iso: eng month: '08' oa_version: None page: 328 - 342 publication: Seminars in Immunology publication_status: published publisher: Academic Press publist_id: '6053' quality_controlled: '1' scopus_import: 1 status: public title: Immune memory in invertebrates type: journal_article user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87 volume: 28 year: '2016' ... --- _id: '1431' abstract: - lang: eng text: The rare socially parasitic butterfly Maculinea alcon occurs in two forms, which are characteristic of hygric or xeric habitats and which exploit different host plants and host ants. The status of these two forms has been the subject of considerable controversy. Populations of the two forms are usually spatially distinct, but at Răscruci in Romania both forms occur on the same site (syntopically). We examined the genetic differentiation between the two forms using eight microsatellite markers, and compared with a nearby hygric site, Şardu. Our results showed that while the two forms are strongly differentiated at Răscruci, it is the xeric form there that is most similar to the hygric form at Şardu, and Bayesian clustering algorithms suggest that these two populations have exchanged genes relatively recently. We found strong evidence for population substructuring, caused by high within host ant nest relatedness, indicating very limited dispersal of most ovipositing females, but not association with particular host ant species. Our results are consistent with the results of larger scale phylogeographic studies that suggest that the two forms represent local ecotypes specialising on different host plants, each with a distinct flowering phenology, providing a temporal rather than spatial barrier to gene flow. article_number: '1865' author: - first_name: András full_name: Tartally, András last_name: Tartally - first_name: Andreas full_name: Kelager, Andreas last_name: Kelager - first_name: Matthias full_name: Fürst, Matthias id: 393B1196-F248-11E8-B48F-1D18A9856A87 last_name: Fürst orcid: 0000-0002-3712-925X - first_name: David full_name: Nash, David last_name: Nash citation: ama: Tartally A, Kelager A, Fürst M, Nash D. Host plant use drives genetic differentiation in syntopic populations of Maculinea alcon. PeerJ. 2016;2016(3). doi:10.7717/peerj.1865 apa: Tartally, A., Kelager, A., Fürst, M., & Nash, D. (2016). Host plant use drives genetic differentiation in syntopic populations of Maculinea alcon. PeerJ. PeerJ. https://doi.org/10.7717/peerj.1865 chicago: Tartally, András, Andreas Kelager, Matthias Fürst, and David Nash. “Host Plant Use Drives Genetic Differentiation in Syntopic Populations of Maculinea Alcon.” PeerJ. PeerJ, 2016. https://doi.org/10.7717/peerj.1865. ieee: A. Tartally, A. Kelager, M. Fürst, and D. Nash, “Host plant use drives genetic differentiation in syntopic populations of Maculinea alcon,” PeerJ, vol. 2016, no. 3. PeerJ, 2016. ista: Tartally A, Kelager A, Fürst M, Nash D. 2016. Host plant use drives genetic differentiation in syntopic populations of Maculinea alcon. PeerJ. 2016(3), 1865. mla: Tartally, András, et al. “Host Plant Use Drives Genetic Differentiation in Syntopic Populations of Maculinea Alcon.” PeerJ, vol. 2016, no. 3, 1865, PeerJ, 2016, doi:10.7717/peerj.1865. short: A. Tartally, A. Kelager, M. Fürst, D. Nash, PeerJ 2016 (2016). date_created: 2018-12-11T11:51:59Z date_published: 2016-01-01T00:00:00Z date_updated: 2021-01-12T06:50:41Z day: '01' ddc: - '570' department: - _id: SyCr doi: 10.7717/peerj.1865 file: - access_level: open_access checksum: c27d898598a1e3d7f629607a309254e1 content_type: application/pdf creator: system date_created: 2018-12-12T10:17:19Z date_updated: 2020-07-14T12:44:53Z file_id: '5272' file_name: IST-2016-584-v1+1_peerj-1865.pdf file_size: 1216360 relation: main_file file_date_updated: 2020-07-14T12:44:53Z has_accepted_license: '1' intvolume: ' 2016' issue: '3' language: - iso: eng month: '01' oa: 1 oa_version: Published Version publication: PeerJ publication_status: published publisher: PeerJ publist_id: '5767' pubrep_id: '584' quality_controlled: '1' scopus_import: 1 status: public title: Host plant use drives genetic differentiation in syntopic populations of Maculinea alcon tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87 volume: 2016 year: '2016' ... --- _id: '9720' abstract: - lang: eng text: 'Summary: Declining populations of bee pollinators are a cause of concern, with major repercussions for biodiversity loss and food security. RNA viruses associated with honeybees represent a potential threat to other insect pollinators, but the extent of this threat is poorly understood. This study aims to attain a detailed understanding of the current and ongoing risk of emerging infectious disease (EID) transmission between managed and wild pollinator species across a wide range of RNA viruses. Within a structured large-scale national survey across 26 independent sites, we quantify the prevalence and pathogen loads of multiple RNA viruses in co-occurring managed honeybee (Apis mellifera) and wild bumblebee (Bombus spp.) populations. We then construct models that compare virus prevalence between wild and managed pollinators. Multiple RNA viruses associated with honeybees are widespread in sympatric wild bumblebee populations. Virus prevalence in honeybees is a significant predictor of virus prevalence in bumblebees, but we remain cautious in speculating over the principle direction of pathogen transmission. We demonstrate species-specific differences in prevalence, indicating significant variation in disease susceptibility or tolerance. Pathogen loads within individual bumblebees may be high and in the case of at least one RNA virus, prevalence is higher in wild bumblebees than in managed honeybee populations. Our findings indicate widespread transmission of RNA viruses between managed and wild bee pollinators, pointing to an interconnected network of potential disease pressures within and among pollinator species. In the context of the biodiversity crisis, our study emphasizes the importance of targeting a wide range of pathogens and defining host associations when considering potential drivers of population decline.' article_processing_charge: No author: - first_name: Dino full_name: Mcmahon, Dino last_name: Mcmahon - first_name: Matthias full_name: Fürst, Matthias id: 393B1196-F248-11E8-B48F-1D18A9856A87 last_name: Fürst orcid: 0000-0002-3712-925X - first_name: Jesicca full_name: Caspar, Jesicca last_name: Caspar - first_name: Panagiotis full_name: Theodorou, Panagiotis last_name: Theodorou - first_name: Mark full_name: Brown, Mark last_name: Brown - first_name: Robert full_name: Paxton, Robert last_name: Paxton citation: ama: 'Mcmahon D, Fürst M, Caspar J, Theodorou P, Brown M, Paxton R. Data from: A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees. 2016. doi:10.5061/dryad.4b565' apa: 'Mcmahon, D., Fürst, M., Caspar, J., Theodorou, P., Brown, M., & Paxton, R. (2016). Data from: A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees. Dryad. https://doi.org/10.5061/dryad.4b565' chicago: 'Mcmahon, Dino, Matthias Fürst, Jesicca Caspar, Panagiotis Theodorou, Mark Brown, and Robert Paxton. “Data from: A Sting in the Spit: Widespread Cross-Infection of Multiple RNA Viruses across Wild and Managed Bees.” Dryad, 2016. https://doi.org/10.5061/dryad.4b565.' ieee: 'D. Mcmahon, M. Fürst, J. Caspar, P. Theodorou, M. Brown, and R. Paxton, “Data from: A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees.” Dryad, 2016.' ista: 'Mcmahon D, Fürst M, Caspar J, Theodorou P, Brown M, Paxton R. 2016. Data from: A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees, Dryad, 10.5061/dryad.4b565.' mla: 'Mcmahon, Dino, et al. Data from: A Sting in the Spit: Widespread Cross-Infection of Multiple RNA Viruses across Wild and Managed Bees. Dryad, 2016, doi:10.5061/dryad.4b565.' short: D. Mcmahon, M. Fürst, J. Caspar, P. Theodorou, M. Brown, R. Paxton, (2016). date_created: 2021-07-26T09:14:19Z date_published: 2016-01-22T00:00:00Z date_updated: 2023-02-23T10:17:25Z day: '22' department: - _id: SyCr doi: 10.5061/dryad.4b565 main_file_link: - open_access: '1' url: https://doi.org/10.5061/dryad.4b565 month: '01' oa: 1 oa_version: Published Version publisher: Dryad related_material: record: - id: '1855' relation: used_in_publication status: public status: public title: 'Data from: A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees' type: research_data_reference user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf year: '2016' ... --- _id: '1262' abstract: - lang: eng text: Emerging infectious diseases (EIDs) have contributed significantly to the current biodiversity crisis, leading to widespread epidemics and population loss. Owing to genetic variation in pathogen virulence, a complete understanding of species decline requires the accurate identification and characterization of EIDs. We explore this issue in the Western honeybee, where increasing mortality of populations in the Northern Hemisphere has caused major concern. Specifically, we investigate the importance of genetic identity of the main suspect in mortality, deformed wing virus (DWV), in driving honeybee loss. Using laboratory experiments and a systematic field survey, we demonstrate that an emerging DWV genotype (DWV-B) is more virulent than the established DWV genotype (DWV-A) and is widespread in the landscape. Furthermore, we show in a simple model that colonies infected with DWV-B collapse sooner than colonies infected with DWV-A. We also identify potential for rapid DWV evolution by revealing extensive genome-wide recombination in vivo. The emergence of DWV-B in naive honeybee populations, including via recombination with DWV-A, could be of significant ecological and economic importance. Our findings emphasize that knowledge of pathogen genetic identity and diversity is critical to understanding drivers of species decline. acknowledgement: "This work was supported by the Federal Ministry of Food, Agriculture and Consumer Protection (Germany): Fit Bee project (grant 511-06.01-28-1-71.007-10), the EU: BeeDoc (grant 244956), iDiv (2013 NGS-Fast Track grant W47004118) and the Insect Pollinators Initiative (IPI grant BB/I000100/1 and BB/I000151/1). The IPI is funded jointly by the Biotechnology and Biological Sciences Research Council, the Department for Environment, Food and Rural Affairs, the Natural Environment Research Council, the Scottish Government and the Wellcome Trust, under the Living with Environmental Change Partnership. We thank A. Abrahams, M. Husemann and A. Soro\r\nfor support in obtaining\r\nV. destructor\r\n-free honeybees; and BBKA\r\nPresident D. Aston for access to records of colony overwinter\r\n2011–2012 mortality in the UK. We also thank the anonymous refe-\r\nrees and Stephen Martin for comments that led to substantial\r\nimprovement of the manuscript." article_number: '20160811' author: - first_name: Dino full_name: Mcmahon, Dino last_name: Mcmahon - first_name: Myrsini full_name: Natsopoulou, Myrsini last_name: Natsopoulou - first_name: Vincent full_name: Doublet, Vincent last_name: Doublet - first_name: Matthias full_name: Fürst, Matthias id: 393B1196-F248-11E8-B48F-1D18A9856A87 last_name: Fürst orcid: 0000-0002-3712-925X - first_name: Silvio full_name: Weging, Silvio last_name: Weging - first_name: Mark full_name: Brown, Mark last_name: Brown - first_name: Andreas full_name: Gogol Döring, Andreas last_name: Gogol Döring - first_name: Robert full_name: Paxton, Robert last_name: Paxton citation: ama: Mcmahon D, Natsopoulou M, Doublet V, et al. Elevated virulence of an emerging viral genotype as a driver of honeybee loss. Proceedings of the Royal Society of London Series B Biological Sciences. 2016;283(1833). doi:10.1098/rspb.2016.0811 apa: Mcmahon, D., Natsopoulou, M., Doublet, V., Fürst, M., Weging, S., Brown, M., … Paxton, R. (2016). Elevated virulence of an emerging viral genotype as a driver of honeybee loss. Proceedings of the Royal Society of London Series B Biological Sciences. Royal Society, The. https://doi.org/10.1098/rspb.2016.0811 chicago: Mcmahon, Dino, Myrsini Natsopoulou, Vincent Doublet, Matthias Fürst, Silvio Weging, Mark Brown, Andreas Gogol Döring, and Robert Paxton. “Elevated Virulence of an Emerging Viral Genotype as a Driver of Honeybee Loss.” Proceedings of the Royal Society of London Series B Biological Sciences. Royal Society, The, 2016. https://doi.org/10.1098/rspb.2016.0811. ieee: D. Mcmahon et al., “Elevated virulence of an emerging viral genotype as a driver of honeybee loss,” Proceedings of the Royal Society of London Series B Biological Sciences, vol. 283, no. 1833. Royal Society, The, 2016. ista: Mcmahon D, Natsopoulou M, Doublet V, Fürst M, Weging S, Brown M, Gogol Döring A, Paxton R. 2016. Elevated virulence of an emerging viral genotype as a driver of honeybee loss. Proceedings of the Royal Society of London Series B Biological Sciences. 283(1833), 20160811. mla: Mcmahon, Dino, et al. “Elevated Virulence of an Emerging Viral Genotype as a Driver of Honeybee Loss.” Proceedings of the Royal Society of London Series B Biological Sciences, vol. 283, no. 1833, 20160811, Royal Society, The, 2016, doi:10.1098/rspb.2016.0811. short: D. Mcmahon, M. Natsopoulou, V. Doublet, M. Fürst, S. Weging, M. Brown, A. Gogol Döring, R. Paxton, Proceedings of the Royal Society of London Series B Biological Sciences 283 (2016). date_created: 2018-12-11T11:51:00Z date_published: 2016-06-29T00:00:00Z date_updated: 2023-02-23T14:05:30Z day: '29' ddc: - '576' - '592' department: - _id: SyCr doi: 10.1098/rspb.2016.0811 file: - access_level: open_access checksum: 0b0d1be38b497d004064650acb3baced content_type: application/pdf creator: system date_created: 2018-12-12T10:08:46Z date_updated: 2020-07-14T12:44:42Z file_id: '4708' file_name: IST-2016-701-v1+1_20160811.full.pdf file_size: 796872 relation: main_file file_date_updated: 2020-07-14T12:44:42Z has_accepted_license: '1' intvolume: ' 283' issue: '1833' language: - iso: eng month: '06' oa: 1 oa_version: Published Version publication: Proceedings of the Royal Society of London Series B Biological Sciences publication_status: published publisher: Royal Society, The publist_id: '6060' pubrep_id: '701' quality_controlled: '1' related_material: record: - id: '9704' relation: research_data status: public scopus_import: 1 status: public title: Elevated virulence of an emerging viral genotype as a driver of honeybee loss tmp: image: /images/cc_by.png legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) short: CC BY (4.0) type: journal_article user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87 volume: 283 year: '2016' ...