---
_id: '10607'
abstract:
- lang: eng
text: The evidence linking innate immunity mechanisms and neurodegenerative diseases
is growing, but the specific mechanisms are incompletely understood. Experimental
data suggest that microglial TLR4 mediates the uptake and clearance of α-synuclein
also termed synucleinophagy. The accumulation of misfolded α-synuclein throughout
the brain is central to Parkinson's disease (PD). The distribution and progression
of the pathology is often attributed to the propagation of α-synuclein. Here,
we apply a classical α-synuclein propagation model of prodromal PD in wild type
and TLR4 deficient mice to study the role of TLR4 in the progression of the disease.
Our data suggest that TLR4 deficiency facilitates the α-synuclein seed spreading
associated with reduced lysosomal activity of microglia. Three months after seed
inoculation, more pronounced proteinase K-resistant α-synuclein inclusion pathology
is observed in mice with TLR4 deficiency. The facilitated propagation of α-synuclein
is associated with early loss of dopamine transporter (DAT) signal in the striatum
and loss of dopaminergic neurons in substantia nigra pars compacta of TLR4 deficient
mice. These new results support TLR4 signaling as a putative target for disease
modification to slow the progression of PD and related disorders.
acknowledgement: This study was supported by grants of the Austrian Science Fund (FWF)
F4414 and W1206-08. Electron microscopy was performed at the Scientific Service
Units (SSU) of IST-Austria through resources provided by the Electron Microscopy
Facility.
article_processing_charge: No
article_type: original
author:
- first_name: Serena
full_name: Venezia, Serena
last_name: Venezia
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Gregor K.
full_name: Wenning, Gregor K.
last_name: Wenning
- first_name: Nadia
full_name: Stefanova, Nadia
last_name: Stefanova
citation:
ama: Venezia S, Kaufmann W, Wenning GK, Stefanova N. Toll-like receptor 4 deficiency
facilitates α-synuclein propagation and neurodegeneration in a mouse model of
prodromal Parkinson’s disease. Parkinsonism & Related Disorders. 2021;91:59-65.
doi:10.1016/j.parkreldis.2021.09.007
apa: Venezia, S., Kaufmann, W., Wenning, G. K., & Stefanova, N. (2021). Toll-like
receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration
in a mouse model of prodromal Parkinson’s disease. Parkinsonism & Related
Disorders. Elsevier. https://doi.org/10.1016/j.parkreldis.2021.09.007
chicago: Venezia, Serena, Walter Kaufmann, Gregor K. Wenning, and Nadia Stefanova.
“Toll-like Receptor 4 Deficiency Facilitates α-Synuclein Propagation and Neurodegeneration
in a Mouse Model of Prodromal Parkinson’s Disease.” Parkinsonism & Related
Disorders. Elsevier, 2021. https://doi.org/10.1016/j.parkreldis.2021.09.007.
ieee: S. Venezia, W. Kaufmann, G. K. Wenning, and N. Stefanova, “Toll-like receptor
4 deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse
model of prodromal Parkinson’s disease,” Parkinsonism & Related Disorders,
vol. 91. Elsevier, pp. 59–65, 2021.
ista: Venezia S, Kaufmann W, Wenning GK, Stefanova N. 2021. Toll-like receptor 4
deficiency facilitates α-synuclein propagation and neurodegeneration in a mouse
model of prodromal Parkinson’s disease. Parkinsonism & Related Disorders.
91, 59–65.
mla: Venezia, Serena, et al. “Toll-like Receptor 4 Deficiency Facilitates α-Synuclein
Propagation and Neurodegeneration in a Mouse Model of Prodromal Parkinson’s Disease.”
Parkinsonism & Related Disorders, vol. 91, Elsevier, 2021, pp. 59–65,
doi:10.1016/j.parkreldis.2021.09.007.
short: S. Venezia, W. Kaufmann, G.K. Wenning, N. Stefanova, Parkinsonism & Related
Disorders 91 (2021) 59–65.
date_created: 2022-01-09T23:01:26Z
date_published: 2021-10-01T00:00:00Z
date_updated: 2023-08-17T06:36:01Z
day: '01'
ddc:
- '610'
department:
- _id: EM-Fac
doi: 10.1016/j.parkreldis.2021.09.007
external_id:
isi:
- '000701142900012'
pmid:
- '34530328'
file:
- access_level: open_access
checksum: 360681585acb51e80d17c6b213c56b55
content_type: application/pdf
creator: alisjak
date_created: 2022-01-10T13:41:40Z
date_updated: 2022-01-10T13:41:40Z
file_id: '10612'
file_name: 2021_Parkinsonism_Venezia.pdf
file_size: 6848513
relation: main_file
success: 1
file_date_updated: 2022-01-10T13:41:40Z
has_accepted_license: '1'
intvolume: ' 91'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '10'
oa: 1
oa_version: Published Version
page: 59-65
pmid: 1
publication: Parkinsonism & Related Disorders
publication_identifier:
eissn:
- 1873-5126
issn:
- 1353-8020
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Toll-like receptor 4 deficiency facilitates α-synuclein propagation and neurodegeneration
in a mouse model of prodromal Parkinson's disease
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 91
year: '2021'
...
---
_id: '9301'
abstract:
- lang: eng
text: Electrodepositing insulating lithium peroxide (Li2O2) is the key process during
discharge of aprotic Li–O2 batteries and determines rate, capacity, and reversibility.
Current understanding states that the partition between surface adsorbed and dissolved
lithium superoxide governs whether Li2O2 grows as a conformal surface film or
larger particles, leading to low or high capacities, respectively. However, better
understanding governing factors for Li2O2 packing density and capacity requires
structural sensitive in situ metrologies. Here, we establish in situ small- and
wide-angle X-ray scattering (SAXS/WAXS) as a suitable method to record the Li2O2
phase evolution with atomic to submicrometer resolution during cycling a custom-built
in situ Li–O2 cell. Combined with sophisticated data analysis, SAXS allows retrieving
rich quantitative structural information from complex multiphase systems. Surprisingly,
we find that features are absent that would point at a Li2O2 surface film formed
via two consecutive electron transfers, even in poorly solvating electrolytes
thought to be prototypical for surface growth. All scattering data can be modeled
by stacks of thin Li2O2 platelets potentially forming large toroidal particles.
Li2O2 solution growth is further justified by rotating ring-disk electrode measurements
and electron microscopy. Higher discharge overpotentials lead to smaller Li2O2
particles, but there is no transition to an electronically passivating, conformal
Li2O2 coating. Hence, mass transport of reactive species rather than electronic
transport through a Li2O2 film limits the discharge capacity. Provided that species
mobilities and carbon surface areas are high, this allows for high discharge capacities
even in weakly solvating electrolytes. The currently accepted Li–O2 reaction mechanism
ought to be reconsidered.
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: S.A.F. and C.P. are indebted to the European Research Council under
the European Union's Horizon 2020 research and innovation program (Grant Agreement
No. 636069), the Austrian Federal Ministry of Science, Research and Economy, and
the Austrian Research Promotion Agency (Grant No. 845364). We acknowledge A. Zankel
and H. Schroettner for support with SEM measurements. C.P. thanks N. Kostoglou,
C. Koczwara, M. Hartmann, and M. Burian for discussions on gas sorption analysis,
C++ programming, Monte Carlo modeling, and in situ SAXS experiments, respectively.
We thank S. Stadlbauer for help with Karl Fischer titration, R. Riccò for gas sorption
measurements, and acknowledge Graz University of Technology for support through
the Lead Project LP-03. Likewise, the use of SOMAPP Lab, a core facility supported
by the Austrian Federal Ministry of Education, Science and Research, the Graz University
of Technology, the University of Graz, and Anton Paar GmbH is acknowledged. S.A.F.
is indebted to Institute of Science and Technology Austria (IST Austria) for support.
This research was supported by the Scientific Service Units of IST Austria through
resources provided by the Electron Microscopy Facility.
article_number: e2021893118
article_processing_charge: No
article_type: original
author:
- first_name: Christian
full_name: Prehal, Christian
last_name: Prehal
- first_name: Aleksej
full_name: Samojlov, Aleksej
last_name: Samojlov
- first_name: Manfred
full_name: Nachtnebel, Manfred
last_name: Nachtnebel
- first_name: Ludek
full_name: Lovicar, Ludek
id: 36DB3A20-F248-11E8-B48F-1D18A9856A87
last_name: Lovicar
orcid: 0000-0001-6206-4200
- first_name: Manfred
full_name: Kriechbaum, Manfred
last_name: Kriechbaum
- first_name: Heinz
full_name: Amenitsch, Heinz
last_name: Amenitsch
- first_name: Stefan Alexander
full_name: Freunberger, Stefan Alexander
id: A8CA28E6-CE23-11E9-AD2D-EC27E6697425
last_name: Freunberger
orcid: 0000-0003-2902-5319
citation:
ama: Prehal C, Samojlov A, Nachtnebel M, et al. In situ small-angle X-ray scattering
reveals solution phase discharge of Li–O2 batteries with weakly solvating electrolytes.
Proceedings of the National Academy of Sciences. 2021;118(14). doi:10.1073/pnas.2021893118
apa: Prehal, C., Samojlov, A., Nachtnebel, M., Lovicar, L., Kriechbaum, M., Amenitsch,
H., & Freunberger, S. A. (2021). In situ small-angle X-ray scattering reveals
solution phase discharge of Li–O2 batteries with weakly solvating electrolytes.
Proceedings of the National Academy of Sciences. National Academy of Sciences.
https://doi.org/10.1073/pnas.2021893118
chicago: Prehal, Christian, Aleksej Samojlov, Manfred Nachtnebel, Ludek Lovicar,
Manfred Kriechbaum, Heinz Amenitsch, and Stefan Alexander Freunberger. “In Situ
Small-Angle X-Ray Scattering Reveals Solution Phase Discharge of Li–O2 Batteries
with Weakly Solvating Electrolytes.” Proceedings of the National Academy of
Sciences. National Academy of Sciences, 2021. https://doi.org/10.1073/pnas.2021893118.
ieee: C. Prehal et al., “In situ small-angle X-ray scattering reveals solution
phase discharge of Li–O2 batteries with weakly solvating electrolytes,” Proceedings
of the National Academy of Sciences, vol. 118, no. 14. National Academy of
Sciences, 2021.
ista: Prehal C, Samojlov A, Nachtnebel M, Lovicar L, Kriechbaum M, Amenitsch H,
Freunberger SA. 2021. In situ small-angle X-ray scattering reveals solution phase
discharge of Li–O2 batteries with weakly solvating electrolytes. Proceedings of
the National Academy of Sciences. 118(14), e2021893118.
mla: Prehal, Christian, et al. “In Situ Small-Angle X-Ray Scattering Reveals Solution
Phase Discharge of Li–O2 Batteries with Weakly Solvating Electrolytes.” Proceedings
of the National Academy of Sciences, vol. 118, no. 14, e2021893118, National
Academy of Sciences, 2021, doi:10.1073/pnas.2021893118.
short: C. Prehal, A. Samojlov, M. Nachtnebel, L. Lovicar, M. Kriechbaum, H. Amenitsch,
S.A. Freunberger, Proceedings of the National Academy of Sciences 118 (2021).
date_created: 2021-03-31T07:00:01Z
date_published: 2021-04-06T00:00:00Z
date_updated: 2023-09-05T13:27:18Z
day: '06'
department:
- _id: StFr
- _id: EM-Fac
doi: 10.1073/pnas.2021893118
external_id:
isi:
- '000637398300050'
intvolume: ' 118'
isi: 1
issue: '14'
keyword:
- small-angle X-ray scattering
- oxygen reduction
- disproportionation
- Li-air battery
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.26434/chemrxiv.11447775
month: '04'
oa: 1
oa_version: Preprint
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
issn:
- 0027-8424
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
status: public
title: In situ small-angle X-ray scattering reveals solution phase discharge of Li–O2
batteries with weakly solvating electrolytes
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 118
year: '2021'
...
---
_id: '10836'
acknowledgement: This work was supported by the Austrian Science Fund (FWF) grants MCCA W1248-B30 and SFB F4606-B28 to EJJ. CP received a short-term
research fellowship of the European Federation of Immunological Societies (EFIS-IL) for a research visit at Biocruces Bizkaia Health Research Institute, Barakaldo, Spain. VKK received an EFIS-IL short-term research fellowship for a research visit at King’s College London. The
research was funded by the National Institute for Health Research (NIHR) Biomedical
Research Centre (BRC) based at Guy's and St Thomas' NHS Foundation Trust and King's
College London (IS-BRC-1215-20006) (SNK). The authors acknowledge support by the Medical Research Council
(MR/L023091/1) (SNK); Breast Cancer Now (147; KCL-BCN-Q3)(SNK); Cancer Research
UK (C30122/A11527; C30122/A15774) (SNK); Cancer Research UK King's Health Partners Centre at King's College London (C604/A25135) (SNK); CRUK/NIHR in England/DoH for Scotland, Wales and Northern Ireland Experimental Cancer Medicine Centre (C10355/A15587) (SNK). The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR or the Department of Health. Additionally, this work was funded by Instituto de Salud Carlos III through the project "PI16/01223" (Co-funded by European
Regional Development Fund; “A way to make Europe”) to FB and by the Department of Health, Basque Government through the project
“2019111031” to OZ. OZ is recipient of a Sara Borrell 2017 post-doctoral contract
“CD17/00128” funded by Instituto de Salud Carlos III (Co-funded by European Social
Fund; “Investing in your future”).
article_processing_charge: No
article_type: letter_note
author:
- first_name: Christina L.
full_name: Pranger, Christina L.
last_name: Pranger
- first_name: Judit
full_name: Fazekas-Singer, Judit
id: 36432834-F248-11E8-B48F-1D18A9856A87
last_name: Fazekas-Singer
orcid: 0000-0002-8777-3502
- first_name: Verena K.
full_name: Köhler, Verena K.
last_name: Köhler
- first_name: Isabella
full_name: Pali‐Schöll, Isabella
last_name: Pali‐Schöll
- first_name: Alessandro
full_name: Fiocchi, Alessandro
last_name: Fiocchi
- first_name: Sophia N.
full_name: Karagiannis, Sophia N.
last_name: Karagiannis
- first_name: Olatz
full_name: Zenarruzabeitia, Olatz
last_name: Zenarruzabeitia
- first_name: Francisco
full_name: Borrego, Francisco
last_name: Borrego
- first_name: Erika
full_name: Jensen‐Jarolim, Erika
last_name: Jensen‐Jarolim
citation:
ama: 'Pranger CL, Singer J, Köhler VK, et al. PIPE‐cloned human IgE and IgG4 antibodies:
New tools for investigating cow’s milk allergy and tolerance. Allergy.
2021;76(5):1553-1556. doi:10.1111/all.14604'
apa: 'Pranger, C. L., Singer, J., Köhler, V. K., Pali‐Schöll, I., Fiocchi, A., Karagiannis,
S. N., … Jensen‐Jarolim, E. (2021). PIPE‐cloned human IgE and IgG4 antibodies:
New tools for investigating cow’s milk allergy and tolerance. Allergy.
Wiley. https://doi.org/10.1111/all.14604'
chicago: 'Pranger, Christina L., Judit Singer, Verena K. Köhler, Isabella Pali‐Schöll,
Alessandro Fiocchi, Sophia N. Karagiannis, Olatz Zenarruzabeitia, Francisco Borrego,
and Erika Jensen‐Jarolim. “PIPE‐cloned Human IgE and IgG4 Antibodies: New Tools
for Investigating Cow’s Milk Allergy and Tolerance.” Allergy. Wiley, 2021.
https://doi.org/10.1111/all.14604.'
ieee: 'C. L. Pranger et al., “PIPE‐cloned human IgE and IgG4 antibodies:
New tools for investigating cow’s milk allergy and tolerance,” Allergy,
vol. 76, no. 5. Wiley, pp. 1553–1556, 2021.'
ista: 'Pranger CL, Singer J, Köhler VK, Pali‐Schöll I, Fiocchi A, Karagiannis SN,
Zenarruzabeitia O, Borrego F, Jensen‐Jarolim E. 2021. PIPE‐cloned human IgE and
IgG4 antibodies: New tools for investigating cow’s milk allergy and tolerance.
Allergy. 76(5), 1553–1556.'
mla: 'Pranger, Christina L., et al. “PIPE‐cloned Human IgE and IgG4 Antibodies:
New Tools for Investigating Cow’s Milk Allergy and Tolerance.” Allergy,
vol. 76, no. 5, Wiley, 2021, pp. 1553–56, doi:10.1111/all.14604.'
short: C.L. Pranger, J. Singer, V.K. Köhler, I. Pali‐Schöll, A. Fiocchi, S.N. Karagiannis,
O. Zenarruzabeitia, F. Borrego, E. Jensen‐Jarolim, Allergy 76 (2021) 1553–1556.
date_created: 2022-03-08T11:19:05Z
date_published: 2021-05-01T00:00:00Z
date_updated: 2023-09-05T15:58:53Z
day: '01'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1111/all.14604
external_id:
isi:
- '000577708800001'
pmid:
- '32990982'
file:
- access_level: open_access
checksum: 9526f9554112fc027c9f7fa540c488cd
content_type: application/pdf
creator: dernst
date_created: 2022-03-08T11:23:16Z
date_updated: 2022-03-08T11:23:16Z
file_id: '10837'
file_name: 2021_Allergy_Pranger.pdf
file_size: 626081
relation: main_file
success: 1
file_date_updated: 2022-03-08T11:23:16Z
has_accepted_license: '1'
intvolume: ' 76'
isi: 1
issue: '5'
keyword:
- Immunology
- Immunology and Allergy
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1553-1556
pmid: 1
publication: Allergy
publication_identifier:
eissn:
- 1398-9995
issn:
- 0105-4538
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'PIPE‐cloned human IgE and IgG4 antibodies: New tools for investigating cow''s
milk allergy and tolerance'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 76
year: '2021'
...
---
_id: '9928'
abstract:
- lang: eng
text: There are two elementary superconducting qubit types that derive directly
from the quantum harmonic oscillator. In one, the inductor is replaced by a nonlinear
Josephson junction to realize the widely used charge qubits with a compact phase
variable and a discrete charge wave function. In the other, the junction is added
in parallel, which gives rise to an extended phase variable, continuous wave functions,
and a rich energy-level structure due to the loop topology. While the corresponding
rf superconducting quantum interference device Hamiltonian was introduced as a
quadratic quasi-one-dimensional potential approximation to describe the fluxonium
qubit implemented with long Josephson-junction arrays, in this work we implement
it directly using a linear superinductor formed by a single uninterrupted aluminum
wire. We present a large variety of qubits, all stemming from the same circuit
but with drastically different characteristic energy scales. This includes flux
and fluxonium qubits but also the recently introduced quasicharge qubit with strongly
enhanced zero-point phase fluctuations and a heavily suppressed flux dispersion.
The use of a geometric inductor results in high reproducibility of the inductive
energy as guaranteed by top-down lithography—a key ingredient for intrinsically
protected superconducting qubits.
acknowledged_ssus:
- _id: NanoFab
- _id: M-Shop
acknowledgement: We thank W. Hughes for analytic and numerical modeling during the
early stages of this work, J. Koch for discussions and support with the scqubits
package, R. Sett, P. Zielinski, and L. Drmic for software development, and G. Katsaros
for equipment support, as well as the MIBA workshop and the Institute of Science
and Technology Austria nanofabrication facility. We thank I. Pop, S. Deleglise,
and E. Flurin for discussions. This work was supported by a NOMIS Foundation research
grant, the Austrian Science Fund (FWF) through BeyondC (F7105), and IST Austria.
M.P. is the recipient of a Pöttinger scholarship at IST Austria. E.R. is the recipient
of a DOC fellowship of the Austrian Academy of Sciences at IST Austria.
article_processing_charge: No
article_type: original
author:
- first_name: Matilda
full_name: Peruzzo, Matilda
id: 3F920B30-F248-11E8-B48F-1D18A9856A87
last_name: Peruzzo
orcid: 0000-0002-3415-4628
- first_name: Farid
full_name: Hassani, Farid
id: 2AED110C-F248-11E8-B48F-1D18A9856A87
last_name: Hassani
orcid: 0000-0001-6937-5773
- first_name: Gregory
full_name: Szep, Gregory
last_name: Szep
- first_name: Andrea
full_name: Trioni, Andrea
id: 42F71B44-F248-11E8-B48F-1D18A9856A87
last_name: Trioni
- first_name: Elena
full_name: Redchenko, Elena
id: 2C21D6E8-F248-11E8-B48F-1D18A9856A87
last_name: Redchenko
- first_name: Martin
full_name: Zemlicka, Martin
id: 2DCF8DE6-F248-11E8-B48F-1D18A9856A87
last_name: Zemlicka
- first_name: Johannes M
full_name: Fink, Johannes M
id: 4B591CBA-F248-11E8-B48F-1D18A9856A87
last_name: Fink
orcid: 0000-0001-8112-028X
citation:
ama: 'Peruzzo M, Hassani F, Szep G, et al. Geometric superinductance qubits: Controlling
phase delocalization across a single Josephson junction. PRX Quantum. 2021;2(4):040341.
doi:10.1103/PRXQuantum.2.040341'
apa: 'Peruzzo, M., Hassani, F., Szep, G., Trioni, A., Redchenko, E., Zemlicka, M.,
& Fink, J. M. (2021). Geometric superinductance qubits: Controlling phase
delocalization across a single Josephson junction. PRX Quantum. American
Physical Society. https://doi.org/10.1103/PRXQuantum.2.040341'
chicago: 'Peruzzo, Matilda, Farid Hassani, Gregory Szep, Andrea Trioni, Elena Redchenko,
Martin Zemlicka, and Johannes M Fink. “Geometric Superinductance Qubits: Controlling
Phase Delocalization across a Single Josephson Junction.” PRX Quantum.
American Physical Society, 2021. https://doi.org/10.1103/PRXQuantum.2.040341.'
ieee: 'M. Peruzzo et al., “Geometric superinductance qubits: Controlling
phase delocalization across a single Josephson junction,” PRX Quantum,
vol. 2, no. 4. American Physical Society, p. 040341, 2021.'
ista: 'Peruzzo M, Hassani F, Szep G, Trioni A, Redchenko E, Zemlicka M, Fink JM.
2021. Geometric superinductance qubits: Controlling phase delocalization across
a single Josephson junction. PRX Quantum. 2(4), 040341.'
mla: 'Peruzzo, Matilda, et al. “Geometric Superinductance Qubits: Controlling Phase
Delocalization across a Single Josephson Junction.” PRX Quantum, vol. 2,
no. 4, American Physical Society, 2021, p. 040341, doi:10.1103/PRXQuantum.2.040341.'
short: M. Peruzzo, F. Hassani, G. Szep, A. Trioni, E. Redchenko, M. Zemlicka, J.M.
Fink, PRX Quantum 2 (2021) 040341.
date_created: 2021-08-17T08:14:18Z
date_published: 2021-11-24T00:00:00Z
date_updated: 2023-09-07T13:31:22Z
day: '24'
ddc:
- '530'
department:
- _id: JoFi
- _id: NanoFab
- _id: M-Shop
doi: 10.1103/PRXQuantum.2.040341
ec_funded: 1
external_id:
arxiv:
- '2106.05882'
isi:
- '000723015100001'
file:
- access_level: open_access
checksum: 36eb41ea43d8ca22b0efab12419e4eb2
content_type: application/pdf
creator: cchlebak
date_created: 2022-01-18T11:29:33Z
date_updated: 2022-01-18T11:29:33Z
file_id: '10641'
file_name: 2021_PRXQuantum_Peruzzo.pdf
file_size: 4247422
relation: main_file
success: 1
file_date_updated: 2022-01-18T11:29:33Z
has_accepted_license: '1'
intvolume: ' 2'
isi: 1
issue: '4'
keyword:
- quantum physics
- mesoscale and nanoscale physics
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
page: '040341'
project:
- _id: 26927A52-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: F07105
name: Integrating superconducting quantum circuits
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 2622978C-B435-11E9-9278-68D0E5697425
name: Hybrid Semiconductor - Superconductor Quantum Devices
publication: PRX Quantum
publication_identifier:
eissn:
- 2691-3399
publication_status: published
publisher: American Physical Society
quality_controlled: '1'
related_material:
record:
- id: '13057'
relation: research_data
status: public
- id: '9920'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'Geometric superinductance qubits: Controlling phase delocalization across
a single Josephson junction'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 2
year: '2021'
...
---
_id: '10223'
abstract:
- lang: eng
text: Growth regulation tailors development in plants to their environment. A prominent
example of this is the response to gravity, in which shoots bend up and roots
bend down1. This paradox is based on opposite effects of the phytohormone auxin,
which promotes cell expansion in shoots while inhibiting it in roots via a yet
unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic
engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding
of how auxin inhibits root growth. We show that auxin activates two distinct,
antagonistically acting signalling pathways that converge on rapid regulation
of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE
KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma
membrane H+-ATPases for apoplast acidification, while intracellular canonical
auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization.
Simultaneous activation of these two counteracting mechanisms poises roots for
rapid, fine-tuned growth modulation in navigating complex soil environments.
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
- _id: Bio
acknowledgement: We thank N. Gnyliukh and L. Hörmayer for technical assistance and
N. Paris for sharing PM-Cyto seeds. We gratefully acknowledge the Life Science,
Machine Shop and Bioimaging Facilities of IST Austria. This project has received
funding from the European Research Council Advanced Grant (ETAP-742985) and the
Austrian Science Fund (FWF) under I 3630-B25 to J.F., the National Institutes of
Health (GM067203) to W.M.G., the Netherlands Organization for Scientific Research
(NWO; VIDI-864.13.001), Research Foundation-Flanders (FWO; Odysseus II G0D0515N)
and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R.,
the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research
to M.R. and D.W., the Australian Research Council and China National Distinguished
Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685)
and T.K. (20H05687 and 20H05910), the European Union’s Horizon 2020 research and
innovation programme under Marie Skłodowska-Curie grant agreement no. 665385 and
the DOC Fellowship of the Austrian Academy of Sciences to L.L., and the China Scholarship
Council to J.C.
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Jian
full_name: Chen, Jian
last_name: Chen
- first_name: Lana
full_name: Shabala, Lana
last_name: Shabala
- first_name: Wouter
full_name: Smet, Wouter
last_name: Smet
- first_name: Hong
full_name: Ren, Hong
last_name: Ren
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Sergey
full_name: Shabala, Sergey
last_name: Shabala
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: William M.
full_name: Gray, William M.
last_name: Gray
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin
signalling for H+ fluxes in root growth. Nature. 2021;599(7884):273-277.
doi:10.1038/s41586-021-04037-6
apa: Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L.,
Merrin, J., … Friml, J. (2021). Cell surface and intracellular auxin signalling
for H+ fluxes in root growth. Nature. Springer Nature. https://doi.org/10.1038/s41586-021-04037-6
chicago: Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez
Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin
Signalling for H+ Fluxes in Root Growth.” Nature. Springer Nature,
2021. https://doi.org/10.1038/s41586-021-04037-6.
ieee: L. Li et al., “Cell surface and intracellular auxin signalling for
H+ fluxes in root growth,” Nature, vol. 599, no. 7884. Springer
Nature, pp. 273–277, 2021.
ista: Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J,
Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D,
Kinoshita T, Gray WM, Friml J. 2021. Cell surface and intracellular auxin signalling
for H+ fluxes in root growth. Nature. 599(7884), 273–277.
mla: Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+
Fluxes in Root Growth.” Nature, vol. 599, no. 7884, Springer Nature, 2021,
pp. 273–77, doi:10.1038/s41586-021-04037-6.
short: L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J.
Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel,
D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Nature 599 (2021) 273–277.
date_created: 2021-11-07T23:01:25Z
date_published: 2021-11-11T00:00:00Z
date_updated: 2023-10-18T08:30:53Z
day: '11'
department:
- _id: JiFr
- _id: NanoFab
doi: 10.1038/s41586-021-04037-6
ec_funded: 1
external_id:
isi:
- '000713338100006'
pmid:
- '34707283'
intvolume: ' 599'
isi: 1
issue: '7884'
keyword:
- Multidisciplinary
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.doi.org/10.21203/rs.3.rs-266395/v3
month: '11'
oa: 1
oa_version: Preprint
page: 273-277
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Nature
publication_identifier:
eissn:
- '14764687'
issn:
- '00280836'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Webpage
relation: press_release
url: https://ist.ac.at/en/news/stop-and-grow/
record:
- id: '10095'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: Cell surface and intracellular auxin signalling for H+ fluxes in
root growth
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 599
year: '2021'
...
---
_id: '9887'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis is the major route of entry of cargos into cells
and thus underpins many physiological processes. During endocytosis, an area of
flat membrane is remodeled by proteins to create a spherical vesicle against intracellular
forces. The protein machinery which mediates this membrane bending in plants is
unknown. However, it is known that plant endocytosis is actin independent, thus
indicating that plants utilize a unique mechanism to mediate membrane bending
against high-turgor pressure compared to other model systems. Here, we investigate
the TPLATE complex, a plant-specific endocytosis protein complex. It has been
thought to function as a classical adaptor functioning underneath the clathrin
coat. However, by using biochemical and advanced live microscopy approaches, we
found that TPLATE is peripherally associated with clathrin-coated vesicles and
localizes at the rim of endocytosis events. As this localization is more fitting
to the protein machinery involved in membrane bending during endocytosis, we examined
cells in which the TPLATE complex was disrupted and found that the clathrin structures
present as flat patches. This suggests a requirement of the TPLATE complex for
membrane bending during plant clathrin–mediated endocytosis. Next, we used in
vitro biophysical assays to confirm that the TPLATE complex possesses protein
domains with intrinsic membrane remodeling activity. These results redefine the
role of the TPLATE complex and implicate it as a key component of the evolutionarily
distinct plant endocytosis mechanism, which mediates endocytic membrane bending
against the high-turgor pressure in plant cells.
acknowledged_ssus:
- _id: EM-Fac
- _id: LifeSc
- _id: Bio
acknowledgement: 'We gratefully thank Julie Neveu and Dr. Amanda Barranco of the Grégory
Vert laboratory for help preparing plants in France, Dr. Zuzana Gelova for help
and advice with protoplast generation, Dr. Stéphane Vassilopoulos and Dr. Florian
Schur for advice regarding EM tomography, Alejandro Marquiegui Alvaro for help with
material generation, and Dr. Lukasz Kowalski for generously gifting us the mWasabi
protein. This research was supported by the Scientific Service Units of Institute
of Science and Technology Austria (IST Austria) through resources provided by the
Electron Microscopy Facility, Lab Support Facility (particularly Dorota Jaworska),
and the Bioimaging Facility. We acknowledge the Advanced Microscopy Facility of
the Vienna BioCenter Core Facilities for use of the 3D SIM. For the mass spectrometry
analysis of proteins, we acknowledge the University of Natural Resources and Life
Sciences (BOKU) Core Facility Mass Spectrometry. This work was supported by the
following funds: A.J. is supported by funding from the Austrian Science Fund I3630B25
to J.F. P.M. and E.B. are supported by Agence Nationale de la Recherche ANR-11-EQPX-0029
Morphoscope2 and ANR-10-INBS-04 France BioImaging. S.Y.B. is supported by the NSF
No. 1121998 and 1614915. J.W. and D.V.D. are supported by the European Research
Council Grant 682436 (to D.V.D.), a China Scholarship Council Grant 201508440249
(to J.W.), and by a Ghent University Special Research Co-funding Grant ST01511051
(to J.W.).'
article_number: e2113046118
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Dana A
full_name: Dahhan, Dana A
last_name: Dahhan
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Tommaso
full_name: Costanzo, Tommaso
id: D93824F4-D9BA-11E9-BB12-F207E6697425
last_name: Costanzo
orcid: 0000-0001-9732-3815
- first_name: Pierre
full_name: Mahou, Pierre
last_name: Mahou
- first_name: Mónika
full_name: Hrtyan, Mónika
id: 45A71A74-F248-11E8-B48F-1D18A9856A87
last_name: Hrtyan
- first_name: Jie
full_name: Wang, Jie
last_name: Wang
- first_name: Juan L
full_name: Aguilera Servin, Juan L
id: 2A67C376-F248-11E8-B48F-1D18A9856A87
last_name: Aguilera Servin
orcid: 0000-0002-2862-8372
- first_name: Daniël
full_name: van Damme, Daniël
last_name: van Damme
- first_name: Emmanuel
full_name: Beaurepaire, Emmanuel
last_name: Beaurepaire
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Sebastian Y
full_name: Bednarek, Sebastian Y
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Dahhan DA, Gnyliukh N, et al. The TPLATE complex mediates membrane
bending during plant clathrin-mediated endocytosis. Proceedings of the National
Academy of Sciences. 2021;118(51). doi:10.1073/pnas.2113046118
apa: Johnson, A. J., Dahhan, D. A., Gnyliukh, N., Kaufmann, W., Zheden, V., Costanzo,
T., … Friml, J. (2021). The TPLATE complex mediates membrane bending during plant
clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences.
National Academy of Sciences. https://doi.org/10.1073/pnas.2113046118
chicago: Johnson, Alexander J, Dana A Dahhan, Nataliia Gnyliukh, Walter Kaufmann,
Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, et al. “The TPLATE Complex Mediates
Membrane Bending during Plant Clathrin-Mediated Endocytosis.” Proceedings of
the National Academy of Sciences. National Academy of Sciences, 2021. https://doi.org/10.1073/pnas.2113046118.
ieee: A. J. Johnson et al., “The TPLATE complex mediates membrane bending
during plant clathrin-mediated endocytosis,” Proceedings of the National Academy
of Sciences, vol. 118, no. 51. National Academy of Sciences, 2021.
ista: Johnson AJ, Dahhan DA, Gnyliukh N, Kaufmann W, Zheden V, Costanzo T, Mahou
P, Hrtyan M, Wang J, Aguilera Servin JL, van Damme D, Beaurepaire E, Loose M,
Bednarek SY, Friml J. 2021. The TPLATE complex mediates membrane bending during
plant clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences.
118(51), e2113046118.
mla: Johnson, Alexander J., et al. “The TPLATE Complex Mediates Membrane Bending
during Plant Clathrin-Mediated Endocytosis.” Proceedings of the National Academy
of Sciences, vol. 118, no. 51, e2113046118, National Academy of Sciences,
2021, doi:10.1073/pnas.2113046118.
short: A.J. Johnson, D.A. Dahhan, N. Gnyliukh, W. Kaufmann, V. Zheden, T. Costanzo,
P. Mahou, M. Hrtyan, J. Wang, J.L. Aguilera Servin, D. van Damme, E. Beaurepaire,
M. Loose, S.Y. Bednarek, J. Friml, Proceedings of the National Academy of Sciences
118 (2021).
date_created: 2021-08-11T14:11:43Z
date_published: 2021-12-14T00:00:00Z
date_updated: 2024-02-19T11:06:09Z
day: '14'
ddc:
- '580'
department:
- _id: JiFr
- _id: MaLo
- _id: EvBe
- _id: EM-Fac
- _id: NanoFab
doi: 10.1073/pnas.2113046118
external_id:
isi:
- '000736417600043'
pmid:
- '34907016'
file:
- access_level: open_access
checksum: 8d01e72e22c4fb1584e72d8601947069
content_type: application/pdf
creator: cchlebak
date_created: 2021-12-15T08:59:40Z
date_updated: 2021-12-15T08:59:40Z
file_id: '10546'
file_name: 2021_PNAS_Johnson.pdf
file_size: 2757340
relation: main_file
success: 1
file_date_updated: 2021-12-15T08:59:40Z
has_accepted_license: '1'
intvolume: ' 118'
isi: 1
issue: '51'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: Proceedings of the National Academy of Sciences
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
related_material:
link:
- relation: earlier_version
url: https://doi.org/10.1101/2021.04.26.441441
record:
- id: '14510'
relation: dissertation_contains
status: public
- id: '14988'
relation: research_data
status: public
status: public
title: The TPLATE complex mediates membrane bending during plant clathrin-mediated
endocytosis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 118
year: '2021'
...
---
_id: '8910'
abstract:
- lang: eng
text: A semiconducting nanowire fully wrapped by a superconducting shell has been
proposed as a platform for obtaining Majorana modes at small magnetic fields.
In this study, we demonstrate that the appearance of subgap states in such structures
is actually governed by the junction region in tunneling spectroscopy measurements
and not the full-shell nanowire itself. Short tunneling regions never show subgap
states, whereas longer junctions always do. This can be understood in terms of
quantum dots forming in the junction and hosting Andreev levels in the Yu-Shiba-Rusinov
regime. The intricate magnetic field dependence of the Andreev levels, through
both the Zeeman and Little-Parks effects, may result in robust zero-bias peaks—features
that could be easily misinterpreted as originating from Majorana zero modes but
are unrelated to topological superconductivity.
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
acknowledgement: The authors thank A. Higginbotham, E. J. H. Lee and F. R. Martins
for helpful discussions. This research was supported by the Scientific Service Units
of IST Austria through resources provided by the MIBA Machine Shop and the nanofabrication
facility; the NOMIS Foundation and Microsoft; the European Union’s Horizon 2020
research and innovation program under the Marie SklodowskaCurie grant agreement
No 844511; the FETOPEN Grant Agreement No. 828948; the European Research Commission
through the grant agreement HEMs-DAM No 716655; the Spanish Ministry of Science
and Innovation through Grants PGC2018-097018-B-I00, PCI2018-093026, FIS2016-80434-P
(AEI/FEDER, EU), RYC2011-09345 (Ram´on y Cajal Programme), and the Mar´ıa de Maeztu
Programme for Units of Excellence in R&D (CEX2018-000805-M); the CSIC Research Platform
on Quantum Technologies PTI-001.
article_number: 82-88
article_processing_charge: No
article_type: original
author:
- first_name: Marco
full_name: Valentini, Marco
id: C0BB2FAC-D767-11E9-B658-BC13E6697425
last_name: Valentini
- first_name: Fernando
full_name: Peñaranda, Fernando
last_name: Peñaranda
- first_name: Andrea C
full_name: Hofmann, Andrea C
id: 340F461A-F248-11E8-B48F-1D18A9856A87
last_name: Hofmann
- first_name: Matthias
full_name: Brauns, Matthias
id: 33F94E3C-F248-11E8-B48F-1D18A9856A87
last_name: Brauns
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Peter
full_name: Krogstrup, Peter
last_name: Krogstrup
- first_name: Pablo
full_name: San-Jose, Pablo
last_name: San-Jose
- first_name: Elsa
full_name: Prada, Elsa
last_name: Prada
- first_name: Ramón
full_name: Aguado, Ramón
last_name: Aguado
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
citation:
ama: Valentini M, Peñaranda F, Hofmann AC, et al. Nontopological zero-bias peaks
in full-shell nanowires induced by flux-tunable Andreev states. Science.
2021;373(6550). doi:10.1126/science.abf1513
apa: Valentini, M., Peñaranda, F., Hofmann, A. C., Brauns, M., Hauschild, R., Krogstrup,
P., … Katsaros, G. (2021). Nontopological zero-bias peaks in full-shell nanowires
induced by flux-tunable Andreev states. Science. American Association for
the Advancement of Science. https://doi.org/10.1126/science.abf1513
chicago: Valentini, Marco, Fernando Peñaranda, Andrea C Hofmann, Matthias Brauns,
Robert Hauschild, Peter Krogstrup, Pablo San-Jose, Elsa Prada, Ramón Aguado, and
Georgios Katsaros. “Nontopological Zero-Bias Peaks in Full-Shell Nanowires Induced
by Flux-Tunable Andreev States.” Science. American Association for the
Advancement of Science, 2021. https://doi.org/10.1126/science.abf1513.
ieee: M. Valentini et al., “Nontopological zero-bias peaks in full-shell
nanowires induced by flux-tunable Andreev states,” Science, vol. 373, no.
6550. American Association for the Advancement of Science, 2021.
ista: Valentini M, Peñaranda F, Hofmann AC, Brauns M, Hauschild R, Krogstrup P,
San-Jose P, Prada E, Aguado R, Katsaros G. 2021. Nontopological zero-bias peaks
in full-shell nanowires induced by flux-tunable Andreev states. Science. 373(6550),
82–88.
mla: Valentini, Marco, et al. “Nontopological Zero-Bias Peaks in Full-Shell Nanowires
Induced by Flux-Tunable Andreev States.” Science, vol. 373, no. 6550, 82–88,
American Association for the Advancement of Science, 2021, doi:10.1126/science.abf1513.
short: M. Valentini, F. Peñaranda, A.C. Hofmann, M. Brauns, R. Hauschild, P. Krogstrup,
P. San-Jose, E. Prada, R. Aguado, G. Katsaros, Science 373 (2021).
date_created: 2020-12-02T10:51:52Z
date_published: 2021-07-02T00:00:00Z
date_updated: 2024-02-21T12:40:09Z
day: '02'
department:
- _id: GeKa
- _id: Bio
doi: 10.1126/science.abf1513
ec_funded: 1
external_id:
arxiv:
- '2008.02348'
isi:
- '000677843100034'
intvolume: ' 373'
isi: 1
issue: '6550'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/2008.02348
month: '07'
oa: 1
oa_version: Submitted Version
project:
- _id: 262116AA-B435-11E9-9278-68D0E5697425
name: Hybrid Semiconductor - Superconductor Quantum Devices
- _id: 26A151DA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '844511'
name: Majorana bound states in Ge/SiGe heterostructures
publication: Science
publication_identifier:
eissn:
- '10959203'
issn:
- '00368075'
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/unfinding-a-split-electron/
record:
- id: '13286'
relation: dissertation_contains
status: public
- id: '9389'
relation: research_data
status: public
scopus_import: '1'
status: public
title: Nontopological zero-bias peaks in full-shell nanowires induced by flux-tunable
Andreev states
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 373
year: '2021'
...
---
_id: '10110'
abstract:
- lang: eng
text: Pattern separation is a fundamental brain computation that converts small
differences in input patterns into large differences in output patterns. Several
synaptic mechanisms of pattern separation have been proposed, including code expansion,
inhibition and plasticity; however, which of these mechanisms play a role in the
entorhinal cortex (EC)–dentate gyrus (DG)–CA3 circuit, a classical pattern separation
circuit, remains unclear. Here we show that a biologically realistic, full-scale
EC–DG–CA3 circuit model, including granule cells (GCs) and parvalbumin-positive
inhibitory interneurons (PV+-INs) in the DG, is an efficient pattern separator.
Both external gamma-modulated inhibition and internal lateral inhibition mediated
by PV+-INs substantially contributed to pattern separation. Both local connectivity
and fast signaling at GC–PV+-IN synapses were important for maximum effectiveness.
Similarly, mossy fiber synapses with conditional detonator properties contributed
to pattern separation. By contrast, perforant path synapses with Hebbian synaptic
plasticity and direct EC–CA3 connection shifted the network towards pattern completion.
Our results demonstrate that the specific properties of cells and synapses optimize
higher-order computations in biological networks and might be useful to improve
the deep learning capabilities of technical networks.
author:
- first_name: José
full_name: Guzmán, José
id: 30CC5506-F248-11E8-B48F-1D18A9856A87
last_name: Guzmán
orcid: 0000-0003-2209-5242
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: 'Claudia '
full_name: 'Espinoza Martinez, Claudia '
id: 31FFEE2E-F248-11E8-B48F-1D18A9856A87
last_name: Espinoza Martinez
orcid: 0000-0003-4710-2082
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Benjamin
full_name: Suter, Benjamin
id: 4952F31E-F248-11E8-B48F-1D18A9856A87
last_name: Suter
orcid: 0000-0002-9885-6936
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. How connectivity
rules and synaptic properties shape the efficacy of pattern separation in the
entorhinal cortex–dentate gyrus–CA3 network. 2021. doi:10.15479/AT:ISTA:10110
apa: Guzmán, J., Schlögl, A., Espinoza Martinez, C., Zhang, X., Suter, B., &
Jonas, P. M. (2021). How connectivity rules and synaptic properties shape the
efficacy of pattern separation in the entorhinal cortex–dentate gyrus–CA3 network.
IST Austria. https://doi.org/10.15479/AT:ISTA:10110
chicago: Guzmán, José, Alois Schlögl, Claudia Espinoza Martinez, Xiaomin Zhang,
Benjamin Suter, and Peter M Jonas. “How Connectivity Rules and Synaptic Properties
Shape the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3
Network.” IST Austria, 2021. https://doi.org/10.15479/AT:ISTA:10110.
ieee: J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, and P. M.
Jonas, “How connectivity rules and synaptic properties shape the efficacy of pattern
separation in the entorhinal cortex–dentate gyrus–CA3 network.” IST Austria, 2021.
ista: Guzmán J, Schlögl A, Espinoza Martinez C, Zhang X, Suter B, Jonas PM. 2021.
How connectivity rules and synaptic properties shape the efficacy of pattern separation
in the entorhinal cortex–dentate gyrus–CA3 network, IST Austria, 10.15479/AT:ISTA:10110.
mla: Guzmán, José, et al. How Connectivity Rules and Synaptic Properties Shape
the Efficacy of Pattern Separation in the Entorhinal Cortex–Dentate Gyrus–CA3
Network. IST Austria, 2021, doi:10.15479/AT:ISTA:10110.
short: J. Guzmán, A. Schlögl, C. Espinoza Martinez, X. Zhang, B. Suter, P.M. Jonas,
(2021).
date_created: 2021-10-08T06:44:22Z
date_published: 2021-12-16T00:00:00Z
date_updated: 2024-03-28T23:30:11Z
day: '16'
ddc:
- '005'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.15479/AT:ISTA:10110
file:
- access_level: open_access
checksum: f92f8931cad0aa7e411c1715337bf408
content_type: application/x-zip-compressed
creator: cchlebak
date_created: 2021-10-08T08:46:04Z
date_updated: 2021-10-08T08:46:04Z
file_id: '10114'
file_name: patternseparation-main (1).zip
file_size: 332990101
relation: main_file
success: 1
file_date_updated: 2021-10-08T08:46:04Z
has_accepted_license: '1'
license: https://opensource.org/licenses/GPL-3.0
month: '12'
oa: 1
publisher: IST Austria
related_material:
link:
- description: News on IST Webpage
relation: press_release
url: https://ist.ac.at/en/news/spot-the-difference/
record:
- id: '10816'
relation: used_for_analysis_in
status: public
status: public
title: How connectivity rules and synaptic properties shape the efficacy of pattern
separation in the entorhinal cortex–dentate gyrus–CA3 network
tmp:
legal_code_url: https://www.gnu.org/licenses/gpl-3.0.en.html
name: GNU General Public License 3.0
short: GPL 3.0
type: software
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2021'
...
---
_id: '9429'
abstract:
- lang: eng
text: De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3
lead to autism spectrum disorder (ASD). In mouse, constitutive haploinsufficiency
leads to motor coordination deficits as well as ASD-relevant social and cognitive
impairments. However, induction of Cul3 haploinsufficiency later in life does
not lead to ASD-relevant behaviors, pointing to an important role of Cul3 during
a critical developmental window. Here we show that Cul3 is essential to regulate
neuronal migration and, therefore, constitutive Cul3 heterozygous mutant mice
display cortical lamination abnormalities. At the molecular level, we found that
Cul3 controls neuronal migration by tightly regulating the amount of Plastin3
(Pls3), a previously unrecognized player of neural migration. Furthermore, we
found that Pls3 cell-autonomously regulates cell migration by regulating actin
cytoskeleton organization, and its levels are inversely proportional to neural
migration speed. Finally, we provide evidence that cellular phenotypes associated
with autism-linked gene haploinsufficiency can be rescued by transcriptional activation
of the intact allele in vitro, offering a proof of concept for a potential therapeutic
approach for ASDs.
acknowledged_ssus:
- _id: PreCl
acknowledgement: We thank A. Coll Manzano, F. Freeman, M. Ladron de Guevara, and A.
Ç. Yahya for technical assistance, S. Deixler, A. Lepold, and A. Schlerka for the
management of our animal colony, as well as M. Schunn and the Preclinical Facility
team for technical assistance. We thank K. Heesom and her team at the University
of Bristol Proteomics Facility for the proteomics sample preparation, data generation,
and analysis support. We thank Y. B. Simon for kindly providing the plasmid for
lentiviral labeling. Further, we thank M. Sixt for his advice regarding cell migration
and the fruitful discussions. This work was supported by the ISTPlus postdoctoral
fellowship (Grant Agreement No. 754411) to B.B., by the European Union’s Horizon
2020 research and innovation program (ERC) grant 715508 (REVERSEAUTISM), and by
the Austrian Science Fund (FWF) to G.N. (DK W1232-B24 and SFB F7807-B) and to J.G.D
(I3600-B27).
article_number: '3058'
article_processing_charge: No
article_type: original
author:
- first_name: Jasmin
full_name: Morandell, Jasmin
id: 4739D480-F248-11E8-B48F-1D18A9856A87
last_name: Morandell
- first_name: Lena A
full_name: Schwarz, Lena A
id: 29A8453C-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Bernadette
full_name: Basilico, Bernadette
id: 36035796-5ACA-11E9-A75E-7AF2E5697425
last_name: Basilico
orcid: 0000-0003-1843-3173
- first_name: Saren
full_name: Tasciyan, Saren
id: 4323B49C-F248-11E8-B48F-1D18A9856A87
last_name: Tasciyan
orcid: 0000-0003-1671-393X
- first_name: Georgi A
full_name: Dimchev, Georgi A
id: 38C393BE-F248-11E8-B48F-1D18A9856A87
last_name: Dimchev
orcid: 0000-0001-8370-6161
- first_name: Armel
full_name: Nicolas, Armel
id: 2A103192-F248-11E8-B48F-1D18A9856A87
last_name: Nicolas
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- first_name: Christoph
full_name: Dotter, Christoph
id: 4C66542E-F248-11E8-B48F-1D18A9856A87
last_name: Dotter
orcid: 0000-0002-9033-9096
- first_name: Lisa
full_name: Knaus, Lisa
id: 3B2ABCF4-F248-11E8-B48F-1D18A9856A87
last_name: Knaus
- first_name: Zoe
full_name: Dobler, Zoe
id: D23090A2-9057-11EA-883A-A8396FC7A38F
last_name: Dobler
- first_name: Emanuele
full_name: Cacci, Emanuele
last_name: Cacci
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
citation:
ama: Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein
homeostasis and cell migration during a critical window of brain development.
Nature Communications. 2021;12(1). doi:10.1038/s41467-021-23123-x
apa: Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Dimchev, G. A.,
Nicolas, A., … Novarino, G. (2021). Cul3 regulates cytoskeleton protein homeostasis
and cell migration during a critical window of brain development. Nature Communications.
Springer Nature. https://doi.org/10.1038/s41467-021-23123-x
chicago: Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan,
Georgi A Dimchev, Armel Nicolas, Christoph M Sommer, et al. “Cul3 Regulates Cytoskeleton
Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.”
Nature Communications. Springer Nature, 2021. https://doi.org/10.1038/s41467-021-23123-x.
ieee: J. Morandell et al., “Cul3 regulates cytoskeleton protein homeostasis
and cell migration during a critical window of brain development,” Nature Communications,
vol. 12, no. 1. Springer Nature, 2021.
ista: Morandell J, Schwarz LA, Basilico B, Tasciyan S, Dimchev GA, Nicolas A, Sommer
CM, Kreuzinger C, Dotter C, Knaus L, Dobler Z, Cacci E, Schur FK, Danzl JG, Novarino
G. 2021. Cul3 regulates cytoskeleton protein homeostasis and cell migration during
a critical window of brain development. Nature Communications. 12(1), 3058.
mla: Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis
and Cell Migration during a Critical Window of Brain Development.” Nature Communications,
vol. 12, no. 1, 3058, Springer Nature, 2021, doi:10.1038/s41467-021-23123-x.
short: J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, G.A. Dimchev, A. Nicolas,
C.M. Sommer, C. Kreuzinger, C. Dotter, L. Knaus, Z. Dobler, E. Cacci, F.K. Schur,
J.G. Danzl, G. Novarino, Nature Communications 12 (2021).
date_created: 2021-05-28T11:49:46Z
date_published: 2021-05-24T00:00:00Z
date_updated: 2024-03-28T23:30:23Z
day: '24'
ddc:
- '572'
department:
- _id: GaNo
- _id: JoDa
- _id: FlSc
- _id: MiSi
- _id: LifeSc
- _id: Bio
doi: 10.1038/s41467-021-23123-x
ec_funded: 1
external_id:
isi:
- '000658769900010'
file:
- access_level: open_access
checksum: 337e0f7959c35ec959984cacdcb472ba
content_type: application/pdf
creator: kschuh
date_created: 2021-05-28T12:39:43Z
date_updated: 2021-05-28T12:39:43Z
file_id: '9430'
file_name: 2021_NatureCommunications_Morandell.pdf
file_size: 9358599
relation: main_file
success: 1
file_date_updated: 2021-05-28T12:39:43Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
issue: '1'
keyword:
- General Biochemistry
- Genetics and Molecular Biology
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 25444568-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '715508'
name: Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo
and in vitro Models
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
- _id: 05A0D778-7A3F-11EA-A408-12923DDC885E
grant_number: F07807
name: Neural stem cells in autism and epilepsy
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
publication: Nature Communications
publication_identifier:
eissn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: press_release
url: https://ist.ac.at/en/news/defective-gene-slows-down-brain-cells/
record:
- id: '7800'
relation: earlier_version
status: public
- id: '12401'
relation: dissertation_contains
status: public
status: public
title: Cul3 regulates cytoskeleton protein homeostasis and cell migration during a
critical window of brain development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...
---
_id: '8909'
abstract:
- lang: eng
text: Spin qubits are considered to be among the most promising candidates for building
a quantum processor. Group IV hole spin qubits have moved into the focus of interest
due to the ease of operation and compatibility with Si technology. In addition,
Ge offers the option for monolithic superconductor-semiconductor integration.
Here we demonstrate a hole spin qubit operating at fields below 10 mT, the critical
field of Al, by exploiting the large out-of-plane hole g-factors in planar Ge
and by encoding the qubit into the singlet-triplet states of a double quantum
dot. We observe electrically controlled X and Z-rotations with tunable frequencies
exceeding 100 MHz and dephasing times of 1μs which we extend beyond 15μs with
echo techniques. These results show that Ge hole singlet triplet qubits outperform
their electronic Si and GaAs based counterparts in speed and coherence, respectively.
In addition, they are on par with Ge single spin qubits, but can be operated at
much lower fields underlining their potential for on chip integration with superconducting
technologies.
acknowledged_ssus:
- _id: M-Shop
- _id: NanoFab
acknowledgement: This research was supported by the Scientific Service Units of Institute
of Science and Technology (IST) Austria through resources provided by the Miba Machine
Shop and the nanofabrication facility, and was made possible with the support of
the NOMIS Foundation. This project has received funding from the European Union’s
Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant
agreements no. 844511 and no. 75441, and by the Austrian Science Fund FWF-P 30207
project. A.B. acknowledges support from the European Union Horizon 2020 FET project
microSPIRE, no. 766955. M. Botifoll and J.A. acknowledge funding from Generalitat
de Catalunya 2017 SGR 327. The Catalan Institute of Nanoscience and Nanotechnology
(ICN2) is supported by the Severo Ochoa programme from the Spanish Ministery of
Economy (MINECO) (grant no. SEV-2017-0706) and is funded by the Catalonian Research
Centre (CERCA) Programme, Generalitat de Catalunya. Part of the present work has
been performed within the framework of the Universitat Autónoma de Barcelona Materials
Science PhD programme. Part of the HAADF scanning transmission electron microscopy
was conducted in the Laboratorio de Microscopias Avanzadas at Instituto de Nanociencia
de Aragon, Universidad de Zaragoza. ICN2 acknowledge support from the Spanish Superior
Council of Scientific Research (CSIC) Research Platform on Quantum Technologies
PTI-001. M.B. acknowledges funding from the Catalan Agency for Management of University
and Research Grants (AGAUR) Generalitat de Catalunya formation of investigators
(FI) PhD grant.
article_processing_charge: No
article_type: original
author:
- first_name: Daniel
full_name: Jirovec, Daniel
id: 4C473F58-F248-11E8-B48F-1D18A9856A87
last_name: Jirovec
orcid: 0000-0002-7197-4801
- first_name: Andrea C
full_name: Hofmann, Andrea C
id: 340F461A-F248-11E8-B48F-1D18A9856A87
last_name: Hofmann
- first_name: Andrea
full_name: Ballabio, Andrea
last_name: Ballabio
- first_name: Philipp M.
full_name: Mutter, Philipp M.
last_name: Mutter
- first_name: Giulio
full_name: Tavani, Giulio
last_name: Tavani
- first_name: Marc
full_name: Botifoll, Marc
last_name: Botifoll
- first_name: Alessandro
full_name: Crippa, Alessandro
id: 1F2B21A2-F6E7-11E9-9B82-F7DBE5697425
last_name: Crippa
orcid: 0000-0002-2968-611X
- first_name: Josip
full_name: Kukucka, Josip
id: 3F5D8856-F248-11E8-B48F-1D18A9856A87
last_name: Kukucka
- first_name: Oliver
full_name: Sagi, Oliver
id: 71616374-A8E9-11E9-A7CA-09ECE5697425
last_name: Sagi
- first_name: Frederico
full_name: Martins, Frederico
id: 38F80F9A-1CB8-11EA-BC76-B49B3DDC885E
last_name: Martins
orcid: 0000-0003-2668-2401
- first_name: Jaime
full_name: Saez Mollejo, Jaime
id: e0390f72-f6e0-11ea-865d-862393336714
last_name: Saez Mollejo
- first_name: Ivan
full_name: Prieto Gonzalez, Ivan
id: 2A307FE2-F248-11E8-B48F-1D18A9856A87
last_name: Prieto Gonzalez
orcid: 0000-0002-7370-5357
- first_name: Maksim
full_name: Borovkov, Maksim
id: 2ac7a0a2-3562-11eb-9256-fbd18ea55087
last_name: Borovkov
- first_name: Jordi
full_name: Arbiol, Jordi
last_name: Arbiol
- first_name: Daniel
full_name: Chrastina, Daniel
last_name: Chrastina
- first_name: Giovanni
full_name: Isella, Giovanni
last_name: Isella
- first_name: Georgios
full_name: Katsaros, Georgios
id: 38DB5788-F248-11E8-B48F-1D18A9856A87
last_name: Katsaros
orcid: 0000-0001-8342-202X
citation:
ama: Jirovec D, Hofmann AC, Ballabio A, et al. A singlet triplet hole spin qubit
in planar Ge. Nature Materials. 2021;20(8):1106–1112. doi:10.1038/s41563-021-01022-2
apa: Jirovec, D., Hofmann, A. C., Ballabio, A., Mutter, P. M., Tavani, G., Botifoll,
M., … Katsaros, G. (2021). A singlet triplet hole spin qubit in planar Ge. Nature
Materials. Springer Nature. https://doi.org/10.1038/s41563-021-01022-2
chicago: Jirovec, Daniel, Andrea C Hofmann, Andrea Ballabio, Philipp M. Mutter,
Giulio Tavani, Marc Botifoll, Alessandro Crippa, et al. “A Singlet Triplet Hole
Spin Qubit in Planar Ge.” Nature Materials. Springer Nature, 2021. https://doi.org/10.1038/s41563-021-01022-2.
ieee: D. Jirovec et al., “A singlet triplet hole spin qubit in planar Ge,”
Nature Materials, vol. 20, no. 8. Springer Nature, pp. 1106–1112, 2021.
ista: Jirovec D, Hofmann AC, Ballabio A, Mutter PM, Tavani G, Botifoll M, Crippa
A, Kukucka J, Sagi O, Martins F, Saez Mollejo J, Prieto Gonzalez I, Borovkov M,
Arbiol J, Chrastina D, Isella G, Katsaros G. 2021. A singlet triplet hole spin
qubit in planar Ge. Nature Materials. 20(8), 1106–1112.
mla: Jirovec, Daniel, et al. “A Singlet Triplet Hole Spin Qubit in Planar Ge.” Nature
Materials, vol. 20, no. 8, Springer Nature, 2021, pp. 1106–1112, doi:10.1038/s41563-021-01022-2.
short: D. Jirovec, A.C. Hofmann, A. Ballabio, P.M. Mutter, G. Tavani, M. Botifoll,
A. Crippa, J. Kukucka, O. Sagi, F. Martins, J. Saez Mollejo, I. Prieto Gonzalez,
M. Borovkov, J. Arbiol, D. Chrastina, G. Isella, G. Katsaros, Nature Materials
20 (2021) 1106–1112.
date_created: 2020-12-02T10:50:47Z
date_published: 2021-08-01T00:00:00Z
date_updated: 2024-03-28T23:30:27Z
day: '01'
department:
- _id: GeKa
- _id: NanoFab
- _id: GradSch
doi: 10.1038/s41563-021-01022-2
ec_funded: 1
external_id:
arxiv:
- '2011.13755'
isi:
- '000657596400001'
intvolume: ' 20'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/2011.13755
month: '08'
oa: 1
oa_version: Preprint
page: 1106–1112
project:
- _id: 26A151DA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '844511'
name: Majorana bound states in Ge/SiGe heterostructures
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 2641CE5E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P30207
name: Hole spin orbit qubits in Ge quantum wells
- _id: 262116AA-B435-11E9-9278-68D0E5697425
name: Hybrid Semiconductor - Superconductor Quantum Devices
publication: Nature Materials
publication_identifier:
eissn:
- 1476-4660
issn:
- 1476-1122
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/quantum-computing-with-holes/
record:
- id: '9323'
relation: research_data
status: public
- id: '10058'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A singlet triplet hole spin qubit in planar Ge
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2021'
...
---
_id: '9756'
abstract:
- lang: eng
text: High-resolution visualization and quantification of membrane proteins contribute
to the understanding of their functions and the roles they play in physiological
and pathological conditions. Sodium dodecyl sulfate-digested freeze-fracture replica
labeling (SDS-FRL) is a powerful electron microscopy method to study quantitatively
the two-dimensional distribution of transmembrane proteins and their tightly associated
proteins. During treatment with SDS, intracellular organelles and proteins not
anchored to the replica are dissolved, whereas integral membrane proteins captured
and stabilized by carbon/platinum deposition remain on the replica. Their intra-
and extracellular domains become exposed on the surface of the replica, facilitating
the accessibility of antibodies and, therefore, providing higher labeling efficiency
than those obtained with other immunoelectron microscopy techniques. In this chapter,
we describe the protocols of SDS-FRL adapted for mammalian brain samples, and
optimization of the SDS treatment to increase the labeling efficiency for quantification
of Cav2.1, the alpha subunit of P/Q-type voltage-dependent calcium channels utilizing
deep learning algorithms.
acknowledgement: This work was supported by the European Union (European Research
Council Advanced grant no. 694539 and Human Brain Project Ref. 720270 to R. S.)
and the Austrian Academy of Sciences (DOC fellowship to D.K.).
alternative_title:
- Neuromethods
article_processing_charge: No
author:
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: David
full_name: Kleindienst, David
id: 42E121A4-F248-11E8-B48F-1D18A9856A87
last_name: Kleindienst
- first_name: Harumi
full_name: Harada, Harumi
id: 2E55CDF2-F248-11E8-B48F-1D18A9856A87
last_name: Harada
orcid: 0000-0001-7429-7896
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: 'Kaufmann W, Kleindienst D, Harada H, Shigemoto R. High-Resolution localization
and quantitation of membrane proteins by SDS-digested freeze-fracture replica
labeling (SDS-FRL). In: Receptor and Ion Channel Detection in the Brain.
Vol 169. Neuromethods. New York: Humana; 2021:267-283. doi:10.1007/978-1-0716-1522-5_19'
apa: 'Kaufmann, W., Kleindienst, D., Harada, H., & Shigemoto, R. (2021). High-Resolution
localization and quantitation of membrane proteins by SDS-digested freeze-fracture
replica labeling (SDS-FRL). In Receptor and Ion Channel Detection in the Brain
(Vol. 169, pp. 267–283). New York: Humana. https://doi.org/10.1007/978-1-0716-1522-5_19'
chicago: 'Kaufmann, Walter, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto.
“High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested
Freeze-Fracture Replica Labeling (SDS-FRL).” In Receptor and Ion Channel Detection
in the Brain, 169:267–83. Neuromethods. New York: Humana, 2021. https://doi.org/10.1007/978-1-0716-1522-5_19.'
ieee: 'W. Kaufmann, D. Kleindienst, H. Harada, and R. Shigemoto, “High-Resolution
localization and quantitation of membrane proteins by SDS-digested freeze-fracture
replica labeling (SDS-FRL),” in Receptor and Ion Channel Detection in the
Brain, vol. 169, New York: Humana, 2021, pp. 267–283.'
ista: 'Kaufmann W, Kleindienst D, Harada H, Shigemoto R. 2021.High-Resolution localization
and quantitation of membrane proteins by SDS-digested freeze-fracture replica
labeling (SDS-FRL). In: Receptor and Ion Channel Detection in the Brain. Neuromethods,
vol. 169, 267–283.'
mla: Kaufmann, Walter, et al. “High-Resolution Localization and Quantitation of
Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL).”
Receptor and Ion Channel Detection in the Brain, vol. 169, Humana, 2021,
pp. 267–83, doi:10.1007/978-1-0716-1522-5_19.
short: W. Kaufmann, D. Kleindienst, H. Harada, R. Shigemoto, in:, Receptor and
Ion Channel Detection in the Brain, Humana, New York, 2021, pp. 267–283.
date_created: 2021-07-30T09:34:56Z
date_published: 2021-07-27T00:00:00Z
date_updated: 2024-03-28T23:30:31Z
day: '27'
ddc:
- '573'
department:
- _id: RySh
- _id: EM-Fac
doi: 10.1007/978-1-0716-1522-5_19
ec_funded: 1
has_accepted_license: '1'
intvolume: ' 169'
keyword:
- 'Freeze-fracture replica: Deep learning'
- Immunogold labeling
- Integral membrane protein
- Electron microscopy
language:
- iso: eng
month: '07'
oa_version: None
page: 267-283
place: New York
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '694539'
name: 'In situ analysis of single channel subunit composition in neurons: physiological
implication in synaptic plasticity and behaviour'
- _id: 25CBA828-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '720270'
name: Human Brain Project Specific Grant Agreement 1 (HBP SGA 1)
publication: ' Receptor and Ion Channel Detection in the Brain'
publication_identifier:
eisbn:
- '9781071615225'
isbn:
- '9781071615218'
publication_status: published
publisher: Humana
quality_controlled: '1'
related_material:
record:
- id: '9562'
relation: dissertation_contains
status: public
series_title: Neuromethods
status: public
title: High-Resolution localization and quantitation of membrane proteins by SDS-digested
freeze-fracture replica labeling (SDS-FRL)
type: book_chapter
user_id: D865714E-FA4E-11E9-B85B-F5C5E5697425
volume: 169
year: '2021'
...
---
_id: '8931'
abstract:
- lang: eng
text: "Auxin is a major plant growth regulator, but current models on auxin perception
and signaling cannot explain the whole plethora of auxin effects, in particular
those associated with rapid responses. A possible candidate for a component of
additional auxin perception mechanisms is the AUXIN BINDING PROTEIN 1 (ABP1),
whose function in planta remains unclear.\r\nHere we combined expression analysis
with gain- and loss-of-function approaches to analyze the role of ABP1 in plant
development. ABP1 shows a broad expression largely overlapping with, but not regulated
by, transcriptional auxin response activity. Furthermore, ABP1 activity is not
essential for the transcriptional auxin signaling. Genetic in planta analysis
revealed that abp1 loss-of-function mutants show largely normal development with
minor defects in bolting. On the other hand, ABP1 gain-of-function alleles show
a broad range of growth and developmental defects, including root and hypocotyl
growth and bending, lateral root and leaf development, bolting, as well as response
to heat stress. At the cellular level, ABP1 gain-of-function leads to impaired
auxin effect on PIN polar distribution and affects BFA-sensitive PIN intracellular
aggregation.\r\nThe gain-of-function analysis suggests a broad, but still mechanistically
unclear involvement of ABP1 in plant development, possibly masked in abp1 loss-of-function
mutants by a functional redundancy."
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We would like to acknowledge Bioimaging and Life Science Facilities
at IST Austria for continuous support and also the Plant Sciences Core Facility
of CEITEC Masaryk University for their support with obtaining a part of the scientific
data. We gratefully acknowledge Lindy Abas for help with ABP1::GFP-ABP1 construct
design. This project has received funding from the European Research Council (ERC)
under the European Union’s Horizon 2020 research and innovation program [grant agreement
no. 742985] and Austrian Science Fund (FWF) [I 3630-B25] to J.F.; DOC Fellowship
of the Austrian Academy of Sciences to L.L.; the European Structural and Investment
Funds, Operational Programme Research, Development and Education - Project „MSCAfellow@MUNI“
[CZ.02.2.69/0.0/0.0/17_050/0008496] to M.P.. This project was also supported by
the Czech Science Foundation [GA 20-20860Y] to M.Z and MEYS CR [project no.CZ.02.1.01/0.0/0.0/16_019/0000738]
to M. Č.
article_number: '110750'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Zuzana
full_name: Gelová, Zuzana
id: 0AE74790-0E0B-11E9-ABC7-1ACFE5697425
last_name: Gelová
orcid: 0000-0003-4783-1752
- first_name: Michelle C
full_name: Gallei, Michelle C
id: 35A03822-F248-11E8-B48F-1D18A9856A87
last_name: Gallei
orcid: 0000-0003-1286-7368
- first_name: Markéta
full_name: Pernisová, Markéta
last_name: Pernisová
- first_name: Géraldine
full_name: Brunoud, Géraldine
last_name: Brunoud
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Zlata
full_name: Pavlovicova, Zlata
last_name: Pavlovicova
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
- first_name: Teva
full_name: Vernoux, Teva
last_name: Vernoux
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Gelová Z, Gallei MC, Pernisová M, et al. Developmental roles of auxin binding
protein 1 in Arabidopsis thaliana. Plant Science. 2021;303. doi:10.1016/j.plantsci.2020.110750
apa: Gelová, Z., Gallei, M. C., Pernisová, M., Brunoud, G., Zhang, X., Glanc, M.,
… Friml, J. (2021). Developmental roles of auxin binding protein 1 in Arabidopsis
thaliana. Plant Science. Elsevier. https://doi.org/10.1016/j.plantsci.2020.110750
chicago: Gelová, Zuzana, Michelle C Gallei, Markéta Pernisová, Géraldine Brunoud,
Xixi Zhang, Matous Glanc, Lanxin Li, et al. “Developmental Roles of Auxin Binding
Protein 1 in Arabidopsis Thaliana.” Plant Science. Elsevier, 2021. https://doi.org/10.1016/j.plantsci.2020.110750.
ieee: Z. Gelová et al., “Developmental roles of auxin binding protein 1 in
Arabidopsis thaliana,” Plant Science, vol. 303. Elsevier, 2021.
ista: Gelová Z, Gallei MC, Pernisová M, Brunoud G, Zhang X, Glanc M, Li L, Michalko
J, Pavlovicova Z, Verstraeten I, Han H, Hajny J, Hauschild R, Čovanová M, Zwiewka
M, Hörmayer L, Fendrych M, Xu T, Vernoux T, Friml J. 2021. Developmental roles
of auxin binding protein 1 in Arabidopsis thaliana. Plant Science. 303, 110750.
mla: Gelová, Zuzana, et al. “Developmental Roles of Auxin Binding Protein 1 in Arabidopsis
Thaliana.” Plant Science, vol. 303, 110750, Elsevier, 2021, doi:10.1016/j.plantsci.2020.110750.
short: Z. Gelová, M.C. Gallei, M. Pernisová, G. Brunoud, X. Zhang, M. Glanc, L.
Li, J. Michalko, Z. Pavlovicova, I. Verstraeten, H. Han, J. Hajny, R. Hauschild,
M. Čovanová, M. Zwiewka, L. Hörmayer, M. Fendrych, T. Xu, T. Vernoux, J. Friml,
Plant Science 303 (2021).
date_created: 2020-12-09T14:48:28Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2024-03-28T23:30:44Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.1016/j.plantsci.2020.110750
ec_funded: 1
external_id:
isi:
- '000614154500001'
pmid:
- '33487339'
file:
- access_level: open_access
checksum: a7f2562bdca62d67dfa88e271b62a629
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T07:49:25Z
date_updated: 2021-02-04T07:49:25Z
file_id: '9083'
file_name: 2021_PlantScience_Gelova.pdf
file_size: 12563728
relation: main_file
success: 1
file_date_updated: 2021-02-04T07:49:25Z
has_accepted_license: '1'
intvolume: ' 303'
isi: 1
keyword:
- Agronomy and Crop Science
- Plant Science
- Genetics
- General Medicine
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Plant Science
publication_identifier:
issn:
- 0168-9452
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '11626'
relation: dissertation_contains
status: public
- id: '10083'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Developmental roles of auxin binding protein 1 in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 303
year: '2021'
...
---
_id: '10095'
abstract:
- lang: eng
text: Growth regulation tailors plant development to its environment. A showcase
is response to gravity, where shoots bend up and roots down1. This paradox is
based on opposite effects of the phytohormone auxin, which promotes cell expansion
in shoots, while inhibiting it in roots via a yet unknown cellular mechanism2.
Here, by combining microfluidics, live imaging, genetic engineering and phospho-proteomics
in Arabidopsis thaliana, we advance our understanding how auxin inhibits root
growth. We show that auxin activates two distinct, antagonistically acting signalling
pathways that converge on the rapid regulation of the apoplastic pH, a causative
growth determinant. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts
with and mediates phosphorylation and activation of plasma membrane H+-ATPases
for apoplast acidification, while intracellular canonical auxin signalling promotes
net cellular H+-influx, causing apoplast alkalinisation. The simultaneous activation
of these two counteracting mechanisms poises the root for a rapid, fine-tuned
growth modulation while navigating complex soil environment.
acknowledged_ssus:
- _id: LifeSc
- _id: M-Shop
- _id: Bio
acknowledgement: We thank Nataliia Gnyliukh and Lukas Hörmayer for technical assistance
and Nadine Paris for sharing PM-Cyto seeds. We gratefully acknowledge Life Science,
Machine Shop and Bioimaging Facilities of IST Austria. This project has received
funding from the European Research Council Advanced Grant (ETAP-742985) and the
Austrian Science Fund (FWF) I 3630-B25 to J.F., the National Institutes of Health
(GM067203) to W.M.G., the Netherlands Organization for Scientific Research (NWO;
VIDI-864.13.001.), the Research Foundation-Flanders (FWO; Odysseus II G0D0515N)
and a European Research Council Starting Grant (TORPEDO-714055) to W.S. and B.D.R.,
the VICI grant (865.14.001) from the Netherlands Organization for Scientific Research
to M.R and D.W., the Australian Research Council and China National Distinguished
Expert Project (WQ20174400441) to S.S., the MEXT/JSPS KAKENHI to K.T. (20K06685)
and T.K. (20H05687 and 20H05910), the European Union’s Horizon 2020 research and
innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385
and the DOC Fellowship of the Austrian Academy of Sciences to L.L., the China Scholarship
Council to J.C.
article_number: '266395'
article_processing_charge: No
author:
- first_name: Lanxin
full_name: Li, Lanxin
id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0002-5607-272X
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Mark
full_name: Roosjen, Mark
last_name: Roosjen
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Lesia
full_name: Rodriguez Solovey, Lesia
id: 3922B506-F248-11E8-B48F-1D18A9856A87
last_name: Rodriguez Solovey
orcid: 0000-0002-7244-7237
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Jian
full_name: Chen, Jian
last_name: Chen
- first_name: Lana
full_name: Shabala, Lana
last_name: Shabala
- first_name: Wouter
full_name: Smet, Wouter
last_name: Smet
- first_name: Hong
full_name: Ren, Hong
last_name: Ren
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Sergey
full_name: Shabala, Sergey
last_name: Shabala
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Toshinori
full_name: Kinoshita, Toshinori
last_name: Kinoshita
- first_name: William M.
full_name: Gray, William M.
last_name: Gray
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li L, Verstraeten I, Roosjen M, et al. Cell surface and intracellular auxin
signalling for H+-fluxes in root growth. Research Square. doi:10.21203/rs.3.rs-266395/v3
apa: Li, L., Verstraeten, I., Roosjen, M., Takahashi, K., Rodriguez Solovey, L.,
Merrin, J., … Friml, J. (n.d.). Cell surface and intracellular auxin signalling
for H+-fluxes in root growth. Research Square. https://doi.org/10.21203/rs.3.rs-266395/v3
chicago: Li, Lanxin, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez
Solovey, Jack Merrin, Jian Chen, et al. “Cell Surface and Intracellular Auxin
Signalling for H+-Fluxes in Root Growth.” Research Square, n.d. https://doi.org/10.21203/rs.3.rs-266395/v3.
ieee: L. Li et al., “Cell surface and intracellular auxin signalling for
H+-fluxes in root growth,” Research Square. .
ista: Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez Solovey L, Merrin J,
Chen J, Shabala L, Smet W, Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D,
Kinoshita T, Gray WM, Friml J. Cell surface and intracellular auxin signalling
for H+-fluxes in root growth. Research Square, 266395.
mla: Li, Lanxin, et al. “Cell Surface and Intracellular Auxin Signalling for H+-Fluxes
in Root Growth.” Research Square, 266395, doi:10.21203/rs.3.rs-266395/v3.
short: L. Li, I. Verstraeten, M. Roosjen, K. Takahashi, L. Rodriguez Solovey, J.
Merrin, J. Chen, L. Shabala, W. Smet, H. Ren, S. Vanneste, S. Shabala, B. De Rybel,
D. Weijers, T. Kinoshita, W.M. Gray, J. Friml, Research Square (n.d.).
date_created: 2021-10-06T08:56:22Z
date_published: 2021-09-09T00:00:00Z
date_updated: 2024-03-28T23:30:44Z
day: '09'
department:
- _id: JiFr
- _id: NanoFab
doi: 10.21203/rs.3.rs-266395/v3
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.doi.org/10.21203/rs.3.rs-266395/v3
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 26B4D67E-B435-11E9-9278-68D0E5697425
grant_number: '25351'
name: 'A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated
Rapid Growth Inhibition in Arabidopsis Root'
publication: Research Square
publication_identifier:
issn:
- 2693-5015
publication_status: accepted
related_material:
record:
- id: '10223'
relation: later_version
status: public
- id: '10083'
relation: dissertation_contains
status: public
status: public
title: Cell surface and intracellular auxin signalling for H+-fluxes in root growth
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2021'
...
---
_id: '8181'
author:
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
citation:
ama: Hauschild R. Amplified centrosomes in dendritic cells promote immune cell effector
functions. 2020. doi:10.15479/AT:ISTA:8181
apa: Hauschild, R. (2020). Amplified centrosomes in dendritic cells promote immune
cell effector functions. IST Austria. https://doi.org/10.15479/AT:ISTA:8181
chicago: Hauschild, Robert. “Amplified Centrosomes in Dendritic Cells Promote Immune
Cell Effector Functions.” IST Austria, 2020. https://doi.org/10.15479/AT:ISTA:8181.
ieee: R. Hauschild, “Amplified centrosomes in dendritic cells promote immune cell
effector functions.” IST Austria, 2020.
ista: Hauschild R. 2020. Amplified centrosomes in dendritic cells promote immune
cell effector functions, IST Austria, 10.15479/AT:ISTA:8181.
mla: Hauschild, Robert. Amplified Centrosomes in Dendritic Cells Promote Immune
Cell Effector Functions. IST Austria, 2020, doi:10.15479/AT:ISTA:8181.
short: R. Hauschild, (2020).
date_created: 2020-07-28T16:24:37Z
date_published: 2020-08-24T00:00:00Z
date_updated: 2021-01-11T15:29:08Z
day: '24'
department:
- _id: Bio
doi: 10.15479/AT:ISTA:8181
file:
- access_level: open_access
checksum: 878c60885ce30afb59a884dd5eef451c
content_type: text/plain
creator: rhauschild
date_created: 2020-08-24T15:43:49Z
date_updated: 2020-08-24T15:43:49Z
file_id: '8290'
file_name: centriolesDistance.m
file_size: 6577
relation: main_file
success: 1
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checksum: 5a93ac7be2b66b28e4bd8b113ee6aade
content_type: text/plain
creator: rhauschild
date_created: 2020-08-24T15:43:52Z
date_updated: 2020-08-24T15:43:52Z
file_id: '8291'
file_name: goTracking.m
file_size: 2680
relation: main_file
success: 1
file_date_updated: 2020-08-24T15:43:52Z
has_accepted_license: '1'
license: https://opensource.org/licenses/BSD-3-Clause
month: '08'
oa: 1
publisher: IST Austria
status: public
title: Amplified centrosomes in dendritic cells promote immune cell effector functions
tmp:
legal_code_url: https://opensource.org/licenses/BSD-3-Clause
name: The 3-Clause BSD License
short: 3-Clause BSD
type: software
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '8294'
abstract:
- lang: eng
text: 'Automated root growth analysis and tracking of root tips. '
author:
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
citation:
ama: Hauschild R. RGtracker. 2020. doi:10.15479/AT:ISTA:8294
apa: Hauschild, R. (2020). RGtracker. IST Austria. https://doi.org/10.15479/AT:ISTA:8294
chicago: Hauschild, Robert. “RGtracker.” IST Austria, 2020. https://doi.org/10.15479/AT:ISTA:8294.
ieee: R. Hauschild, “RGtracker.” IST Austria, 2020.
ista: Hauschild R. 2020. RGtracker, IST Austria, 10.15479/AT:ISTA:8294.
mla: Hauschild, Robert. RGtracker. IST Austria, 2020, doi:10.15479/AT:ISTA:8294.
short: R. Hauschild, (2020).
date_created: 2020-08-25T12:52:48Z
date_published: 2020-09-10T00:00:00Z
date_updated: 2021-01-12T08:17:56Z
day: '10'
ddc:
- '570'
department:
- _id: Bio
doi: 10.15479/AT:ISTA:8294
file:
- access_level: open_access
checksum: 108352149987ac6f066e4925bd56e35e
content_type: text/plain
creator: rhauschild
date_created: 2020-09-08T14:26:31Z
date_updated: 2020-09-08T14:26:31Z
file_id: '8346'
file_name: readme.txt
file_size: 882
relation: main_file
success: 1
- access_level: open_access
checksum: ffd6c643b28e0cc7c6d0060a18a7e8ea
content_type: application/octet-stream
creator: rhauschild
date_created: 2020-09-08T14:26:33Z
date_updated: 2020-09-08T14:26:33Z
file_id: '8347'
file_name: RGtracker.mlappinstall
file_size: 246121
relation: main_file
success: 1
file_date_updated: 2020-09-08T14:26:33Z
has_accepted_license: '1'
month: '09'
oa: 1
publisher: IST Austria
status: public
title: RGtracker
tmp:
legal_code_url: https://opensource.org/licenses/BSD-3-Clause
name: The 3-Clause BSD License
short: 3-Clause BSD
type: software
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '8695'
abstract:
- lang: eng
text: A look at international activities on Open Science reveals a broad spectrum
from individual institutional policies to national action plans. The present Recommendations
for a National Open Science Strategy in Austria are based on these international
initiatives and present practical considerations for their coordinated implementation
with regard to strategic developments in research, technology and innovation (RTI)
in Austria until 2030. They are addressed to all relevant actors in the RTI system,
in particular to Research Performing Organisations, Research Funding Organisations,
Research Policy, memory institutions such as Libraries and Researchers. The recommendation
paper was developed from 2018 to 2020 by the OANA working group "Open Science
Strategy" and published for the first time in spring 2020 for a public consultation.
The now available final version of the recommendation document, which contains
feedback and comments from the consultation, is intended to provide an impetus
for further discussion and implementation of Open Science in Austria and serves
as a contribution and basis for a potential national Open Science Strategy in
Austria. The document builds on the diverse expertise of the authors (academia,
administration, library and archive, information technology, science policy, funding
system, etc.) and reflects their personal experiences and opinions.
- lang: ger
text: Der Blick auf internationale Aktivitäten zu Open Science zeigt ein breites
Spektrum von einzelnen institutionellen Policies bis hin zu nationalen Aktionsplänen.
Die vorliegenden Empfehlungen für eine nationale Open Science Strategie in Österreich
orientieren sich an diesen internationalen Initiativen und stellen praktische
Überlegungen für ihre koordinierte Implementierung im Hinblick auf strategische
Entwicklungen in Forschung, Technologie und Innovation (FTI) bis 2030 in Österreich
dar. Dabei richten sie sich an alle relevanten Akteur*innen im FTI System, im
Besonderen an Forschungsstätten, Forschungsförderer, Forschungspolitik, Gedächtnisinstitutionen
wie Bibliotheken und Wissenschafter*innen. Das Empfehlungspapier wurde von 2018
bis 2020 von der OANA-Arbeitsgruppe "Open Science Strategie" entwickelt und im
Frühling 2020 das erste Mal für eine öffentliche Konsultation veröffentlicht.
Die nun vorliegende finale Version des Empfehlungsdokuments, die Feedback und
Kommentare aus der Konsultation enthält, soll ein Anstoß für die weitere Diskussion
und Umsetzung von Open Science in Österreich sein und als Beitrag und Grundlage
einer potentiellen nationalen Open Science Strategie in Österreich dienen. Das
Dokument baut auf der vielfältigen Expertise der Autor*innen auf (Wissenschaft,
Administration, Bibliothek und Archiv, Informationstechnologie, Wissenschaftspolitik,
Förderwesen etc.) und spiegelt deren persönliche Erfahrungen und Meinung wider.
article_processing_charge: No
author:
- first_name: Katja
full_name: Mayer, Katja
last_name: Mayer
- first_name: Katharina
full_name: Rieck, Katharina
last_name: Rieck
- first_name: Stefan
full_name: Reichmann, Stefan
last_name: Reichmann
- first_name: Patrick
full_name: Danowski, Patrick
id: 2EBD1598-F248-11E8-B48F-1D18A9856A87
last_name: Danowski
orcid: 0000-0002-6026-4409
- first_name: Anton
full_name: Graschopf, Anton
last_name: Graschopf
- first_name: Thomas
full_name: König, Thomas
last_name: König
- first_name: Peter
full_name: Kraker, Peter
last_name: Kraker
- first_name: Patrick
full_name: Lehner, Patrick
last_name: Lehner
- first_name: Falk
full_name: Reckling, Falk
last_name: Reckling
- first_name: Tony
full_name: Ross-Hellauer, Tony
last_name: Ross-Hellauer
- first_name: Daniel
full_name: Spichtinger, Daniel
last_name: Spichtinger
- first_name: Michalis
full_name: Tzatzanis, Michalis
last_name: Tzatzanis
- first_name: Stefanie
full_name: Schürz, Stefanie
last_name: Schürz
citation:
ama: Mayer K, Rieck K, Reichmann S, et al. Empfehlungen für eine nationale Open
Science Strategie in Österreich / Recommendations for a National Open Science
Strategy in Austria. OANA; 2020. doi:10.5281/ZENODO.4109242
apa: Mayer, K., Rieck, K., Reichmann, S., Danowski, P., Graschopf, A., König, T.,
… Schürz, S. (2020). Empfehlungen für eine nationale Open Science Strategie
in Österreich / Recommendations for a National Open Science Strategy in Austria.
OANA. https://doi.org/10.5281/ZENODO.4109242
chicago: Mayer, Katja, Katharina Rieck, Stefan Reichmann, Patrick Danowski, Anton
Graschopf, Thomas König, Peter Kraker, et al. Empfehlungen für eine nationale
Open Science Strategie in Österreich / Recommendations for a National Open Science
Strategy in Austria. OANA, 2020. https://doi.org/10.5281/ZENODO.4109242.
ieee: K. Mayer et al., Empfehlungen für eine nationale Open Science Strategie
in Österreich / Recommendations for a National Open Science Strategy in Austria.
OANA, 2020.
ista: Mayer K, Rieck K, Reichmann S, Danowski P, Graschopf A, König T, Kraker P,
Lehner P, Reckling F, Ross-Hellauer T, Spichtinger D, Tzatzanis M, Schürz S. 2020.
Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations
for a National Open Science Strategy in Austria, OANA, 36p.
mla: Mayer, Katja, et al. Empfehlungen für eine nationale Open Science Strategie
in Österreich / Recommendations for a National Open Science Strategy in Austria.
OANA, 2020, doi:10.5281/ZENODO.4109242.
short: K. Mayer, K. Rieck, S. Reichmann, P. Danowski, A. Graschopf, T. König, P.
Kraker, P. Lehner, F. Reckling, T. Ross-Hellauer, D. Spichtinger, M. Tzatzanis,
S. Schürz, Empfehlungen für eine nationale Open Science Strategie in Österreich
/ Recommendations for a National Open Science Strategy in Austria, OANA, 2020.
date_created: 2020-10-23T09:08:28Z
date_published: 2020-10-21T00:00:00Z
date_updated: 2020-10-23T09:34:40Z
day: '21'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.5281/ZENODO.4109242
file:
- access_level: open_access
checksum: 8eba912bb4b20b4f82f8010f2110461a
content_type: application/pdf
creator: dernst
date_created: 2020-10-23T09:29:45Z
date_updated: 2020-10-23T09:29:45Z
file_id: '8696'
file_name: 2020_OANA_Mayer.pdf
file_size: 2298363
relation: main_file
success: 1
file_date_updated: 2020-10-23T09:29:45Z
has_accepted_license: '1'
language:
- iso: ger
month: '10'
oa: 1
oa_version: Published Version
page: '36'
publication_status: published
publisher: OANA
status: public
title: Empfehlungen für eine nationale Open Science Strategie in Österreich / Recommendations
for a National Open Science Strategy in Austria
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: working_paper
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '8706'
abstract:
- lang: eng
text: As part of the Austrian Transition to Open Access (AT2OA) project, subproject
TP1-B is working on designing a monitoring solution for the output of Open Access
publications in Austria. This report on a potential Open Access monitoring approach
in Austria is one of the results of these efforts and can serve as a basis for
discussion on an international level.
- lang: ger
text: Als Teil des Hochschulraumstrukturmittel-Projekts Austrian Transition to Open
Access (AT2OA) befasst sich das Teilprojekt TP1-B mit der Konzeption einer Monitoring-Lösung
für den Open Access-Publikationsoutput in Österreich. Der nun vorliegende Bericht
zu einem potentiellen Open Access-Monitoring in Österreich ist eines der Ergebnisse
dieser Bemühungen und kann als Grundlage einer Diskussion auf internationaler
Ebene dienen.
article_processing_charge: No
article_type: original
author:
- first_name: Patrick
full_name: Danowski, Patrick
id: 2EBD1598-F248-11E8-B48F-1D18A9856A87
last_name: Danowski
orcid: 0000-0002-6026-4409
- first_name: Andreas
full_name: Ferus, Andreas
last_name: Ferus
- first_name: Anna-Laetitia
full_name: Hikl, Anna-Laetitia
last_name: Hikl
- first_name: Gerda
full_name: McNeill, Gerda
last_name: McNeill
- first_name: Clemens
full_name: Miniberger, Clemens
last_name: Miniberger
- first_name: Steve
full_name: Reding, Steve
last_name: Reding
- first_name: Tobias
full_name: Zarka, Tobias
last_name: Zarka
- first_name: Michael
full_name: Zojer, Michael
last_name: Zojer
citation:
ama: Danowski P, Ferus A, Hikl A-L, et al. „Recommendation“ for the further procedure
for open access monitoring. Deliverable of the AT2OA subproject TP1-B. Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 2020;73(2):278-284.
doi:10.31263/voebm.v73i2.3941
apa: Danowski, P., Ferus, A., Hikl, A.-L., McNeill, G., Miniberger, C., Reding,
S., … Zojer, M. (2020). „Recommendation“ for the further procedure for open access
monitoring. Deliverable of the AT2OA subproject TP1-B. Mitteilungen der Vereinigung
Österreichischer Bibliothekarinnen und Bibliothekare. Vereinigung Osterreichischer
Bibliothekarinnen und Bibliothekare. https://doi.org/10.31263/voebm.v73i2.3941
chicago: Danowski, Patrick, Andreas Ferus, Anna-Laetitia Hikl, Gerda McNeill, Clemens
Miniberger, Steve Reding, Tobias Zarka, and Michael Zojer. “„Recommendation“ for
the further procedure for open access monitoring. Deliverable of the AT2OA subproject
TP1-B.” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und
Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare,
2020. https://doi.org/10.31263/voebm.v73i2.3941.
ieee: P. Danowski et al., “„Recommendation“ for the further procedure for
open access monitoring. Deliverable of the AT2OA subproject TP1-B,” Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol.
73, no. 2. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, pp.
278–284, 2020.
ista: Danowski P, Ferus A, Hikl A-L, McNeill G, Miniberger C, Reding S, Zarka T,
Zojer M. 2020. „Recommendation“ for the further procedure for open access monitoring.
Deliverable of the AT2OA subproject TP1-B. Mitteilungen der Vereinigung Österreichischer
Bibliothekarinnen und Bibliothekare. 73(2), 278–284.
mla: Danowski, Patrick, et al. “„Recommendation“ for the further procedure for open
access monitoring. Deliverable of the AT2OA subproject TP1-B.” Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol.
73, no. 2, Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, 2020,
pp. 278–84, doi:10.31263/voebm.v73i2.3941.
short: P. Danowski, A. Ferus, A.-L. Hikl, G. McNeill, C. Miniberger, S. Reding,
T. Zarka, M. Zojer, Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen
und Bibliothekare 73 (2020) 278–284.
date_created: 2020-10-25T23:01:19Z
date_published: 2020-07-14T00:00:00Z
date_updated: 2021-01-12T08:20:40Z
day: '14'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.31263/voebm.v73i2.3941
file:
- access_level: open_access
checksum: 37443c34d91d5bdbeb38c78b14792537
content_type: application/pdf
creator: kschuh
date_created: 2020-10-27T16:27:25Z
date_updated: 2020-10-27T16:27:25Z
file_id: '8714'
file_name: 2020_VOEB_Danowski.pdf
file_size: 960317
relation: main_file
success: 1
file_date_updated: 2020-10-27T16:27:25Z
has_accepted_license: '1'
intvolume: ' 73'
issue: '2'
language:
- iso: ger
month: '07'
oa: 1
oa_version: Published Version
page: 278-284
publication: Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare
publication_identifier:
eissn:
- '10222588'
publication_status: published
publisher: Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare
quality_controlled: '1'
scopus_import: '1'
status: public
title: „Recommendation“ for the further procedure for open access monitoring. Deliverable
of the AT2OA subproject TP1-B
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 73
year: '2020'
...
---
_id: '7474'
abstract:
- lang: eng
text: This booklet is a collection of abstracts presented at the AHPC conference.
article_processing_charge: No
citation:
ama: 'Schlögl A, Kiss J, Elefante S, eds. Austrian High-Performance-Computing
Meeting (AHPC2020). Klosterneuburg, Austria: IST Austria; 2020. doi:10.15479/AT:ISTA:7474'
apa: 'Schlögl, A., Kiss, J., & Elefante, S. (Eds.). (2020). Austrian High-Performance-Computing
meeting (AHPC2020). Presented at the AHPC: Austrian High-Performance-Computing
Meeting, Klosterneuburg, Austria: IST Austria. https://doi.org/10.15479/AT:ISTA:7474'
chicago: 'Schlögl, Alois, Janos Kiss, and Stefano Elefante, eds. Austrian High-Performance-Computing
Meeting (AHPC2020). Klosterneuburg, Austria: IST Austria, 2020. https://doi.org/10.15479/AT:ISTA:7474.'
ieee: 'A. Schlögl, J. Kiss, and S. Elefante, Eds., Austrian High-Performance-Computing
meeting (AHPC2020). Klosterneuburg, Austria: IST Austria, 2020.'
ista: 'Schlögl A, Kiss J, Elefante S eds. 2020. Austrian High-Performance-Computing
meeting (AHPC2020), Klosterneuburg, Austria: IST Austria, 72p.'
mla: Schlögl, Alois, et al., editors. Austrian High-Performance-Computing Meeting
(AHPC2020). IST Austria, 2020, doi:10.15479/AT:ISTA:7474.
short: A. Schlögl, J. Kiss, S. Elefante, eds., Austrian High-Performance-Computing
Meeting (AHPC2020), IST Austria, Klosterneuburg, Austria, 2020.
conference:
end_date: 2020-02-21
location: Klosterneuburg, Austria
name: 'AHPC: Austrian High-Performance-Computing Meeting'
start_date: 2020-02-19
date_created: 2020-02-11T07:59:04Z
date_published: 2020-02-19T00:00:00Z
date_updated: 2023-05-16T07:48:28Z
day: '19'
ddc:
- '000'
department:
- _id: ScienComp
doi: 10.15479/AT:ISTA:7474
editor:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Janos
full_name: Kiss, Janos
id: 3D3A06F8-F248-11E8-B48F-1D18A9856A87
last_name: Kiss
- first_name: Stefano
full_name: Elefante, Stefano
id: 490F40CE-F248-11E8-B48F-1D18A9856A87
last_name: Elefante
file:
- access_level: open_access
checksum: 49798edb9e57bbd6be18362d1d7b18a9
content_type: application/pdf
creator: schloegl
date_created: 2020-02-19T06:53:38Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7504'
file_name: BOOKLET_AHPC2020.final.pdf
file_size: 90899507
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: '72'
place: Klosterneuburg, Austria
publication_identifier:
isbn:
- 978-3-99078-004-6
publication_status: published
publisher: IST Austria
quality_controlled: '1'
status: public
title: Austrian High-Performance-Computing meeting (AHPC2020)
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: book_editor
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '7490'
abstract:
- lang: eng
text: In plants, clathrin mediated endocytosis (CME) represents the major route
for cargo internalisation from the cell surface. It has been assumed to operate
in an evolutionary conserved manner as in yeast and animals. Here we report characterisation
of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement
in electron microscopy and quantitative live imaging techniques. Arabidopsis CME
appears to follow the constant curvature model and the bona fide CME population
generates vesicles of a predominantly hexagonal-basket type; larger and with faster
kinetics than in other models. Contrary to the existing paradigm, actin is dispensable
for CME events at the plasma membrane but plays a unique role in collecting endocytic
vesicles, sorting of internalised cargos and directional endosome movement that
itself actively promote CME events. Internalized vesicles display a strongly delayed
and sequential uncoating. These unique features highlight the independent evolution
of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
article_number: e52067
article_processing_charge: No
article_type: original
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Roshan
full_name: Prizak, Roshan
id: 4456104E-F248-11E8-B48F-1D18A9856A87
last_name: Prizak
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Barbara E
full_name: Casillas Perez, Barbara E
id: 351ED2AA-F248-11E8-B48F-1D18A9856A87
last_name: Casillas Perez
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Narasimhan M, Johnson AJ, Prizak R, et al. Evolutionarily unique mechanistic
framework of clathrin-mediated endocytosis in plants. eLife. 2020;9. doi:10.7554/eLife.52067
apa: Narasimhan, M., Johnson, A. J., Prizak, R., Kaufmann, W., Tan, S., Casillas
Perez, B. E., & Friml, J. (2020). Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants. ELife. eLife Sciences Publications.
https://doi.org/10.7554/eLife.52067
chicago: Narasimhan, Madhumitha, Alexander J Johnson, Roshan Prizak, Walter Kaufmann,
Shutang Tan, Barbara E Casillas Perez, and Jiří Friml. “Evolutionarily Unique
Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” ELife.
eLife Sciences Publications, 2020. https://doi.org/10.7554/eLife.52067.
ieee: M. Narasimhan et al., “Evolutionarily unique mechanistic framework
of clathrin-mediated endocytosis in plants,” eLife, vol. 9. eLife Sciences
Publications, 2020.
ista: Narasimhan M, Johnson AJ, Prizak R, Kaufmann W, Tan S, Casillas Perez BE,
Friml J. 2020. Evolutionarily unique mechanistic framework of clathrin-mediated
endocytosis in plants. eLife. 9, e52067.
mla: Narasimhan, Madhumitha, et al. “Evolutionarily Unique Mechanistic Framework
of Clathrin-Mediated Endocytosis in Plants.” ELife, vol. 9, e52067, eLife
Sciences Publications, 2020, doi:10.7554/eLife.52067.
short: M. Narasimhan, A.J. Johnson, R. Prizak, W. Kaufmann, S. Tan, B.E. Casillas
Perez, J. Friml, ELife 9 (2020).
date_created: 2020-02-16T23:00:50Z
date_published: 2020-01-23T00:00:00Z
date_updated: 2023-08-18T06:33:07Z
day: '23'
ddc:
- '570'
- '580'
department:
- _id: JiFr
- _id: GaTk
- _id: EM-Fac
- _id: SyCr
doi: 10.7554/eLife.52067
ec_funded: 1
external_id:
isi:
- '000514104100001'
pmid:
- '31971511'
file:
- access_level: open_access
checksum: 2052daa4be5019534f3a42f200a09f32
content_type: application/pdf
creator: dernst
date_created: 2020-02-18T07:21:16Z
date_updated: 2020-07-14T12:47:59Z
file_id: '7494'
file_name: 2020_eLife_Narasimhan.pdf
file_size: 7247468
relation: main_file
file_date_updated: 2020-07-14T12:47:59Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: eLife
publication_identifier:
eissn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis
in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7792'
abstract:
- lang: eng
text: Phonon polaritons—light coupled to lattice vibrations—in polar van der Waals
crystals are promising candidates for controlling the flow of energy on the nanoscale
due to their strong field confinement, anisotropic propagation and ultra-long
lifetime in the picosecond range1,2,3,4,5. However, the lack of tunability of
their narrow and material-specific spectral range—the Reststrahlen band—severely
limits their technological implementation. Here, we demonstrate that intercalation
of Na atoms in the van der Waals semiconductor α-V2O5 enables a broad spectral
shift of Reststrahlen bands, and that the phonon polaritons excited show ultra-low
losses (lifetime of 4 ± 1 ps), similar to phonon polaritons in a non-intercalated
crystal (lifetime of 6 ± 1 ps). We expect our intercalation method to be applicable
to other van der Waals crystals, opening the door for the use of phonon polaritons
in broad spectral bands in the mid-infrared domain.
acknowledgement: J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa
Program from the Government of the Principality of Asturias (nos. PA-18-PF-BP17-126
and PA-20-PF-BP19-053, respectively). J.M.-S. acknowledges finantial support from
the Clarín Programme from the Government of the Principality of Asturias and a Marie
Curie-COFUND grant (PA-18-ACB17-29) and the Ramón y Cajal Program from the Government
of Spain (RYC2018-026196-I). K.C., X.P.A.G., H.V. and M.H.B. acknowledge the Air
Force Office of Scientific Research (AFOSR) grant no. FA 9550-18-1-0030 for funding
support. I.E. acknowledges financial support from the Spanish Ministry of Economy
and Competitiveness (grant no. FIS2016-76617-P). A.Y.N. acknowledges the Spanish
Ministry of Science, Innovation and Universities (national project no. MAT2017-88358-C3-3-R)
and the Basque Government (grant no. IT1164-19). Q.B. acknowledges the support from
Australian Research Council (grant nos. FT150100450, IH150100006 and CE170100039).
R.H. acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness
(national project RTI2018-094830-B-100 and the Project MDM-2016-0618 of the María
de Maeztu Units of Excellence Program) and the Basque Goverment (grant no. IT1164-19).
P.A.-G. acknowledges support from the European Research Council under starting grant
no. 715496, 2DNANOPTICA.
article_processing_charge: No
article_type: original
author:
- first_name: Javier
full_name: Taboada-Gutiérrez, Javier
last_name: Taboada-Gutiérrez
- first_name: Gonzalo
full_name: Álvarez-Pérez, Gonzalo
last_name: Álvarez-Pérez
- first_name: Jiahua
full_name: Duan, Jiahua
last_name: Duan
- first_name: Weiliang
full_name: Ma, Weiliang
last_name: Ma
- first_name: Kyle
full_name: Crowley, Kyle
last_name: Crowley
- first_name: Ivan
full_name: Prieto Gonzalez, Ivan
id: 2A307FE2-F248-11E8-B48F-1D18A9856A87
last_name: Prieto Gonzalez
orcid: 0000-0002-7370-5357
- first_name: Andrei
full_name: Bylinkin, Andrei
last_name: Bylinkin
- first_name: Marta
full_name: Autore, Marta
last_name: Autore
- first_name: Halyna
full_name: Volkova, Halyna
last_name: Volkova
- first_name: Kenta
full_name: Kimura, Kenta
last_name: Kimura
- first_name: Tsuyoshi
full_name: Kimura, Tsuyoshi
last_name: Kimura
- first_name: M. H.
full_name: Berger, M. H.
last_name: Berger
- first_name: Shaojuan
full_name: Li, Shaojuan
last_name: Li
- first_name: Qiaoliang
full_name: Bao, Qiaoliang
last_name: Bao
- first_name: Xuan P.A.
full_name: Gao, Xuan P.A.
last_name: Gao
- first_name: Ion
full_name: Errea, Ion
last_name: Errea
- first_name: Alexey Y.
full_name: Nikitin, Alexey Y.
last_name: Nikitin
- first_name: Rainer
full_name: Hillenbrand, Rainer
last_name: Hillenbrand
- first_name: Javier
full_name: Martín-Sánchez, Javier
last_name: Martín-Sánchez
- first_name: Pablo
full_name: Alonso-González, Pablo
last_name: Alonso-González
citation:
ama: Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, et al. Broad spectral tuning
of ultra-low-loss polaritons in a van der Waals crystal by intercalation. Nature
Materials. 2020;19:964–968. doi:10.1038/s41563-020-0665-0
apa: Taboada-Gutiérrez, J., Álvarez-Pérez, G., Duan, J., Ma, W., Crowley, K., Prieto
Gonzalez, I., … Alonso-González, P. (2020). Broad spectral tuning of ultra-low-loss
polaritons in a van der Waals crystal by intercalation. Nature Materials.
Springer Nature. https://doi.org/10.1038/s41563-020-0665-0
chicago: Taboada-Gutiérrez, Javier, Gonzalo Álvarez-Pérez, Jiahua Duan, Weiliang
Ma, Kyle Crowley, Ivan Prieto Gonzalez, Andrei Bylinkin, et al. “Broad Spectral
Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.”
Nature Materials. Springer Nature, 2020. https://doi.org/10.1038/s41563-020-0665-0.
ieee: J. Taboada-Gutiérrez et al., “Broad spectral tuning of ultra-low-loss
polaritons in a van der Waals crystal by intercalation,” Nature Materials,
vol. 19. Springer Nature, pp. 964–968, 2020.
ista: Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, Ma W, Crowley K, Prieto Gonzalez
I, Bylinkin A, Autore M, Volkova H, Kimura K, Kimura T, Berger MH, Li S, Bao Q,
Gao XPA, Errea I, Nikitin AY, Hillenbrand R, Martín-Sánchez J, Alonso-González
P. 2020. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals
crystal by intercalation. Nature Materials. 19, 964–968.
mla: Taboada-Gutiérrez, Javier, et al. “Broad Spectral Tuning of Ultra-Low-Loss
Polaritons in a van Der Waals Crystal by Intercalation.” Nature Materials,
vol. 19, Springer Nature, 2020, pp. 964–968, doi:10.1038/s41563-020-0665-0.
short: J. Taboada-Gutiérrez, G. Álvarez-Pérez, J. Duan, W. Ma, K. Crowley, I. Prieto
Gonzalez, A. Bylinkin, M. Autore, H. Volkova, K. Kimura, T. Kimura, M.H. Berger,
S. Li, Q. Bao, X.P.A. Gao, I. Errea, A.Y. Nikitin, R. Hillenbrand, J. Martín-Sánchez,
P. Alonso-González, Nature Materials 19 (2020) 964–968.
date_created: 2020-05-03T22:00:49Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2023-08-21T06:18:20Z
day: '01'
department:
- _id: NanoFab
doi: 10.1038/s41563-020-0665-0
external_id:
isi:
- '000526218500004'
pmid:
- '32284598'
intvolume: ' 19'
isi: 1
language:
- iso: eng
month: '09'
oa_version: None
page: 964–968
pmid: 1
publication: Nature Materials
publication_identifier:
eissn:
- '14764660'
issn:
- '14761122'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal
by intercalation
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 19
year: '2020'
...
---
_id: '7875'
abstract:
- lang: eng
text: 'Cells navigating through complex tissues face a fundamental challenge: while
multiple protrusions explore different paths, the cell needs to avoid entanglement.
How a cell surveys and then corrects its own shape is poorly understood. Here,
we demonstrate that spatially distinct microtubule dynamics regulate amoeboid
cell migration by locally promoting the retraction of protrusions. In migrating
dendritic cells, local microtubule depolymerization within protrusions remote
from the microtubule organizing center triggers actomyosin contractility controlled
by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin
localization, thereby causing two effects that rate-limit locomotion: (1) impaired
cell edge coordination during path finding and (2) defective adhesion resolution.
Compromised shape control is particularly hindering in geometrically complex microenvironments,
where it leads to entanglement and ultimately fragmentation of the cell body.
We thus demonstrate that microtubules can act as a proprioceptive device: they
sense cell shape and control actomyosin retraction to sustain cellular coherence.'
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
- _id: PreCl
acknowledgement: "The authors thank the Scientific Service Units (Life Sciences, Bioimaging,
Preclinical) of the Institute of Science and Technology Austria for excellent support.
This work was funded by the European Research Council (ERC StG 281556 and CoG 724373),
two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20
to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O.
Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from
the People Program (Marie Curie Actions) of the European Union’s Seventh Framework
Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734)
and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014)
co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier
by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s
Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian
Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and
Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry
of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European
Funds for Social and Regional Development."
article_number: e201907154
article_processing_charge: No
article_type: original
author:
- first_name: Aglaja
full_name: Kopf, Aglaja
id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
last_name: Kopf
orcid: 0000-0002-2187-6656
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Irute
full_name: Girkontaite, Irute
last_name: Girkontaite
- first_name: Kerry
full_name: Tedford, Kerry
last_name: Tedford
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Oliver
full_name: Thorn-Seshold, Oliver
last_name: Thorn-Seshold
- first_name: Dirk
full_name: Trauner, Dirk
id: E8F27F48-3EBA-11E9-92A1-B709E6697425
last_name: Trauner
- first_name: Hans
full_name: Häcker, Hans
last_name: Häcker
- first_name: Klaus Dieter
full_name: Fischer, Klaus Dieter
last_name: Fischer
- first_name: Eva
full_name: Kiermaier, Eva
id: 3EB04B78-F248-11E8-B48F-1D18A9856A87
last_name: Kiermaier
orcid: 0000-0001-6165-5738
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape
and coherence in amoeboid migrating cells. The Journal of Cell Biology.
2020;219(6). doi:10.1083/jcb.201907154
apa: Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin,
J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in
amoeboid migrating cells. The Journal of Cell Biology. Rockefeller University
Press. https://doi.org/10.1083/jcb.201907154
chicago: Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry
Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular
Shape and Coherence in Amoeboid Migrating Cells.” The Journal of Cell Biology.
Rockefeller University Press, 2020. https://doi.org/10.1083/jcb.201907154.
ieee: A. Kopf et al., “Microtubules control cellular shape and coherence
in amoeboid migrating cells,” The Journal of Cell Biology, vol. 219, no.
6. Rockefeller University Press, 2020.
ista: Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold
O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control
cellular shape and coherence in amoeboid migrating cells. The Journal of Cell
Biology. 219(6), e201907154.
mla: Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in
Amoeboid Migrating Cells.” The Journal of Cell Biology, vol. 219, no. 6,
e201907154, Rockefeller University Press, 2020, doi:10.1083/jcb.201907154.
short: A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin,
O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt,
The Journal of Cell Biology 219 (2020).
date_created: 2020-05-24T22:00:56Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2023-08-21T06:28:17Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
- _id: Bio
- _id: NanoFab
doi: 10.1083/jcb.201907154
ec_funded: 1
external_id:
isi:
- '000538141100020'
pmid:
- '32379884'
file:
- access_level: open_access
checksum: cb0b9c77842ae1214caade7b77e4d82d
content_type: application/pdf
creator: dernst
date_created: 2020-11-24T13:25:13Z
date_updated: 2020-11-24T13:25:13Z
file_id: '8801'
file_name: 2020_JCellBiol_Kopf.pdf
file_size: 7536712
relation: main_file
success: 1
file_date_updated: 2020-11-24T13:25:13Z
has_accepted_license: '1'
intvolume: ' 219'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '724373'
name: Cellular navigation along spatial gradients
- _id: 26018E70-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29911
name: Mechanical adaptation of lamellipodial actin
- _id: 252C3B08-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W 1250-B20
name: Nano-Analytics of Cellular Systems
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 25A48D24-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1396-2014
name: Molecular and system level view of immune cell migration
publication: The Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Microtubules control cellular shape and coherence in amoeboid migrating cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 219
year: '2020'
...
---
_id: '7888'
abstract:
- lang: eng
text: Embryonic stem cell cultures are thought to self-organize into embryoid bodies,
able to undergo symmetry-breaking, germ layer specification and even morphogenesis.
Yet, it is unclear how to reconcile this remarkable self-organization capacity
with classical experiments demonstrating key roles for extrinsic biases by maternal
factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish
embryonic tissue explants, prepared prior to germ layer induction and lacking
extraembryonic tissues, can specify all germ layers and form a seemingly complete
mesendoderm anlage. Importantly, explant organization requires polarized inheritance
of maternal factors from dorsal-marginal regions of the blastoderm. Moreover,
induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels,
is highly variable in explants, reminiscent of embryos with reduced Nodal signals
from the extraembryonic tissues. Together, these data suggest that zebrafish explants
do not undergo bona fide self-organization, but rather display features of genetically
encoded self-assembly, where intrinsic genetic programs control the emergence
of order.
article_number: e55190
article_processing_charge: No
article_type: original
author:
- first_name: Alexandra
full_name: Schauer, Alexandra
id: 30A536BA-F248-11E8-B48F-1D18A9856A87
last_name: Schauer
orcid: 0000-0001-7659-9142
- first_name: Diana C
full_name: Nunes Pinheiro, Diana C
id: 2E839F16-F248-11E8-B48F-1D18A9856A87
last_name: Nunes Pinheiro
orcid: 0000-0003-4333-7503
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. Zebrafish embryonic
explants undergo genetically encoded self-assembly. eLife. 2020;9. doi:10.7554/elife.55190
apa: Schauer, A., Nunes Pinheiro, D. C., Hauschild, R., & Heisenberg, C.-P.
J. (2020). Zebrafish embryonic explants undergo genetically encoded self-assembly.
ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.55190
chicago: Schauer, Alexandra, Diana C Nunes Pinheiro, Robert Hauschild, and Carl-Philipp
J Heisenberg. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.”
ELife. eLife Sciences Publications, 2020. https://doi.org/10.7554/elife.55190.
ieee: A. Schauer, D. C. Nunes Pinheiro, R. Hauschild, and C.-P. J. Heisenberg, “Zebrafish
embryonic explants undergo genetically encoded self-assembly,” eLife, vol.
9. eLife Sciences Publications, 2020.
ista: Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. 2020. Zebrafish
embryonic explants undergo genetically encoded self-assembly. eLife. 9, e55190.
mla: Schauer, Alexandra, et al. “Zebrafish Embryonic Explants Undergo Genetically
Encoded Self-Assembly.” ELife, vol. 9, e55190, eLife Sciences Publications,
2020, doi:10.7554/elife.55190.
short: A. Schauer, D.C. Nunes Pinheiro, R. Hauschild, C.-P.J. Heisenberg, ELife
9 (2020).
date_created: 2020-05-25T15:01:40Z
date_published: 2020-04-06T00:00:00Z
date_updated: 2023-08-21T06:25:49Z
day: '06'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.7554/elife.55190
ec_funded: 1
external_id:
isi:
- '000531544400001'
pmid:
- '32250246'
file:
- access_level: open_access
checksum: f6aad884cf706846ae9357fcd728f8b5
content_type: application/pdf
creator: dernst
date_created: 2020-05-25T15:15:43Z
date_updated: 2020-07-14T12:48:04Z
file_id: '7890'
file_name: 2020_eLife_Schauer.pdf
file_size: 7744848
relation: main_file
file_date_updated: 2020-07-14T12:48:04Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
grant_number: '25239'
name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
grant_number: ALTF 850-2017
name: Coordination of mesendoderm cell fate specification and internalization during
zebrafish gastrulation
- _id: 266BC5CE-B435-11E9-9278-68D0E5697425
grant_number: LT000429
name: Coordination of mesendoderm fate specification and internalization during
zebrafish gastrulation
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
record:
- id: '12891'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Zebrafish embryonic explants undergo genetically encoded self-assembly
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2020'
...
---
_id: '7864'
abstract:
- lang: eng
text: "Purpose of review: Cancer is one of the leading causes of death and the incidence
rates are constantly rising. The heterogeneity of tumors poses a big challenge
for the treatment of the disease and natural antibodies additionally affect disease
progression. The introduction of engineered mAbs for anticancer immunotherapies
has substantially improved progression-free and overall survival of cancer patients,
but little efforts have been made to exploit other antibody isotypes than IgG.\r\nRecent
findings: In order to improve these therapies, ‘next-generation antibodies’ were
engineered to enhance a specific feature of classical antibodies and form a group
of highly effective and precise therapy compounds. Advanced antibody approaches
include among others antibody-drug conjugates, glyco-engineered and Fc-engineered
antibodies, antibody fragments, radioimmunotherapy compounds, bispecific antibodies
and alternative (non-IgG) immunoglobulin classes, especially IgE.\r\nSummary:
The current review describes solutions for the needs of next-generation antibody
therapies through different approaches. Careful selection of the best-suited engineering
methodology is a key factor in developing personalized, more specific and more
efficient mAbs against cancer to improve the outcomes of cancer patients. We highlight
here the large evidence of IgE exploiting a highly cytotoxic effector arm as potential
next-generation anticancer immunotherapy."
article_processing_charge: No
article_type: original
author:
- first_name: Judit
full_name: Singer, Judit
id: 36432834-F248-11E8-B48F-1D18A9856A87
last_name: Singer
orcid: 0000-0002-8777-3502
- first_name: Josef
full_name: Singer, Josef
last_name: Singer
- first_name: Erika
full_name: Jensen-Jarolim, Erika
last_name: Jensen-Jarolim
citation:
ama: 'Singer J, Singer J, Jensen-Jarolim E. Precision medicine in clinical oncology:
the journey from IgG antibody to IgE. Current opinion in allergy and clinical
immunology. 2020;20(3):282-289. doi:10.1097/ACI.0000000000000637'
apa: 'Singer, J., Singer, J., & Jensen-Jarolim, E. (2020). Precision medicine
in clinical oncology: the journey from IgG antibody to IgE. Current Opinion
in Allergy and Clinical Immunology. Wolters Kluwer. https://doi.org/10.1097/ACI.0000000000000637'
chicago: 'Singer, Judit, Josef Singer, and Erika Jensen-Jarolim. “Precision Medicine
in Clinical Oncology: The Journey from IgG Antibody to IgE.” Current Opinion
in Allergy and Clinical Immunology. Wolters Kluwer, 2020. https://doi.org/10.1097/ACI.0000000000000637.'
ieee: 'J. Singer, J. Singer, and E. Jensen-Jarolim, “Precision medicine in clinical
oncology: the journey from IgG antibody to IgE,” Current opinion in allergy
and clinical immunology, vol. 20, no. 3. Wolters Kluwer, pp. 282–289, 2020.'
ista: 'Singer J, Singer J, Jensen-Jarolim E. 2020. Precision medicine in clinical
oncology: the journey from IgG antibody to IgE. Current opinion in allergy and
clinical immunology. 20(3), 282–289.'
mla: 'Singer, Judit, et al. “Precision Medicine in Clinical Oncology: The Journey
from IgG Antibody to IgE.” Current Opinion in Allergy and Clinical Immunology,
vol. 20, no. 3, Wolters Kluwer, 2020, pp. 282–89, doi:10.1097/ACI.0000000000000637.'
short: J. Singer, J. Singer, E. Jensen-Jarolim, Current Opinion in Allergy and Clinical
Immunology 20 (2020) 282–289.
date_created: 2020-05-17T22:00:44Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2023-08-21T06:28:52Z
day: '01'
department:
- _id: Bio
doi: 10.1097/ACI.0000000000000637
external_id:
isi:
- '000561358300010'
intvolume: ' 20'
isi: 1
issue: '3'
language:
- iso: eng
month: '06'
oa_version: None
page: 282-289
publication: Current opinion in allergy and clinical immunology
publication_identifier:
eissn:
- '14736322'
publication_status: published
publisher: Wolters Kluwer
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Precision medicine in clinical oncology: the journey from IgG antibody to
IgE'
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2020'
...
---
_id: '8261'
abstract:
- lang: eng
text: Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal
CA3 region, but how they process spatial information remains enigmatic. To examine
the role of GCs in spatial coding, we measured excitatory postsynaptic potentials
(EPSPs) and action potentials (APs) in head-fixed mice running on a linear belt.
Intracellular recording from morphologically identified GCs revealed that most
cells were active, but activity level varied over a wide range. Whereas only ∼5%
of GCs showed spatially tuned spiking, ∼50% received spatially tuned input. Thus,
the GC population broadly encodes spatial information, but only a subset relays
this information to the CA3 network. Fourier analysis indicated that GCs received
conjunctive place-grid-like synaptic input, suggesting code conversion in single
neurons. GC firing was correlated with dendritic complexity and intrinsic excitability,
but not extrinsic excitatory input or dendritic cable properties. Thus, functional
maturation may control input-output transformation and spatial code conversion.
acknowledged_ssus:
- _id: M-Shop
- _id: ScienComp
- _id: PreCl
acknowledgement: This project has received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation program (grant
agreement 692692, P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung
(Z 312-B27, Wittgenstein award, P.J.). We thank Gyorgy Buzsáki, Jozsef Csicsvari,
Juan Ramirez Villegas, and Federico Stella for commenting on earlier versions of
this manuscript. We also thank Katie Bittner, Michael Brecht, Albert Lee, Jeffery
Magee, and Alejandro Pernía-Andrade for sharing expertise in in vivo patch-clamp
recording. We are grateful to Florian Marr for cell labeling, cell reconstruction,
and technical assistance; Ben Suter for helpful discussions; Christina Altmutter
for technical support; Eleftheria Kralli-Beller for manuscript editing; and Todor
Asenov (Machine Shop) for device construction. We also thank the Scientific Service
Units (SSUs) of IST Austria (Machine Shop, Scientific Computing, and Preclinical
Facility) for efficient support.
article_processing_charge: No
article_type: original
author:
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Zhang X, Schlögl A, Jonas PM. Selective routing of spatial information flow
from input to output in hippocampal granule cells. Neuron. 2020;107(6):1212-1225.
doi:10.1016/j.neuron.2020.07.006
apa: Zhang, X., Schlögl, A., & Jonas, P. M. (2020). Selective routing of spatial
information flow from input to output in hippocampal granule cells. Neuron.
Elsevier. https://doi.org/10.1016/j.neuron.2020.07.006
chicago: Zhang, Xiaomin, Alois Schlögl, and Peter M Jonas. “Selective Routing of
Spatial Information Flow from Input to Output in Hippocampal Granule Cells.” Neuron.
Elsevier, 2020. https://doi.org/10.1016/j.neuron.2020.07.006.
ieee: X. Zhang, A. Schlögl, and P. M. Jonas, “Selective routing of spatial information
flow from input to output in hippocampal granule cells,” Neuron, vol. 107,
no. 6. Elsevier, pp. 1212–1225, 2020.
ista: Zhang X, Schlögl A, Jonas PM. 2020. Selective routing of spatial information
flow from input to output in hippocampal granule cells. Neuron. 107(6), 1212–1225.
mla: Zhang, Xiaomin, et al. “Selective Routing of Spatial Information Flow from
Input to Output in Hippocampal Granule Cells.” Neuron, vol. 107, no. 6,
Elsevier, 2020, pp. 1212–25, doi:10.1016/j.neuron.2020.07.006.
short: X. Zhang, A. Schlögl, P.M. Jonas, Neuron 107 (2020) 1212–1225.
date_created: 2020-08-14T09:36:05Z
date_published: 2020-09-23T00:00:00Z
date_updated: 2023-08-22T08:30:55Z
day: '23'
ddc:
- '570'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.1016/j.neuron.2020.07.006
ec_funded: 1
external_id:
isi:
- '000579698700009'
pmid:
- '32763145'
file:
- access_level: open_access
checksum: 44a5960fc083a4cb3488d22224859fdc
content_type: application/pdf
creator: dernst
date_created: 2020-12-04T09:29:21Z
date_updated: 2020-12-04T09:29:21Z
file_id: '8920'
file_name: 2020_Neuron_Zhang.pdf
file_size: 3011120
relation: main_file
success: 1
file_date_updated: 2020-12-04T09:29:21Z
has_accepted_license: '1'
intvolume: ' 107'
isi: 1
issue: '6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
page: 1212-1225
pmid: 1
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Website
relation: press_release
url: https://ist.ac.at/en/news/the-bouncer-in-the-brain/
status: public
title: Selective routing of spatial information flow from input to output in hippocampal
granule cells
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 107
year: '2020'
...
---
_id: '8597'
abstract:
- lang: eng
text: Error analysis and data visualization of positive COVID-19 cases in 27 countries
have been performed up to August 8, 2020. This survey generally observes a progression
from early exponential growth transitioning to an intermediate power-law growth
phase, as recently suggested by Ziff and Ziff. The occurrence of logistic growth
after the power-law phase with lockdowns or social distancing may be described
as an effect of avoidance. A visualization of the power-law growth exponent over
short time windows is qualitatively similar to the Bhatia visualization for pandemic
progression. Visualizations like these can indicate the onset of second waves
and may influence social policy.
acknowledgement: I would especially like to thank Michael Sixt for encouraging me
to think about these problems while working at home due to restrictions in place.
I want to thank Nick Barton, Katka Bodova, Matthew Robinson, Simon Rella, Federico
Sau, Ivan Prieto, and Pradeep Kumar for useful discussions.
article_number: '065005'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
citation:
ama: Merrin J. Differences in power law growth over time and indicators of COVID-19
pandemic progression worldwide. Physical Biology. 2020;17(6). doi:10.1088/1478-3975/abb2db
apa: Merrin, J. (2020). Differences in power law growth over time and indicators
of COVID-19 pandemic progression worldwide. Physical Biology. IOP Publishing.
https://doi.org/10.1088/1478-3975/abb2db
chicago: Merrin, Jack. “Differences in Power Law Growth over Time and Indicators
of COVID-19 Pandemic Progression Worldwide.” Physical Biology. IOP Publishing,
2020. https://doi.org/10.1088/1478-3975/abb2db.
ieee: J. Merrin, “Differences in power law growth over time and indicators of COVID-19
pandemic progression worldwide,” Physical Biology, vol. 17, no. 6. IOP
Publishing, 2020.
ista: Merrin J. 2020. Differences in power law growth over time and indicators of
COVID-19 pandemic progression worldwide. Physical Biology. 17(6), 065005.
mla: Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of
COVID-19 Pandemic Progression Worldwide.” Physical Biology, vol. 17, no.
6, 065005, IOP Publishing, 2020, doi:10.1088/1478-3975/abb2db.
short: J. Merrin, Physical Biology 17 (2020).
date_created: 2020-10-04T22:01:35Z
date_published: 2020-09-23T00:00:00Z
date_updated: 2023-08-22T09:53:29Z
day: '23'
ddc:
- '510'
- '570'
department:
- _id: NanoFab
doi: 10.1088/1478-3975/abb2db
external_id:
isi:
- '000575539700001'
file:
- access_level: open_access
checksum: fec9bdd355ed349f09990faab20838a7
content_type: application/pdf
creator: dernst
date_created: 2020-10-05T13:53:59Z
date_updated: 2020-10-05T13:53:59Z
file_id: '8609'
file_name: 2020_PhysBio_Merrin.pdf
file_size: 1667111
relation: main_file
success: 1
file_date_updated: 2020-10-05T13:53:59Z
has_accepted_license: '1'
intvolume: ' 17'
isi: 1
issue: '6'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication: Physical Biology
publication_identifier:
eissn:
- '14783975'
publication_status: published
publisher: IOP Publishing
quality_controlled: '1'
scopus_import: '1'
status: public
title: Differences in power law growth over time and indicators of COVID-19 pandemic
progression worldwide
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 17
year: '2020'
...
---
_id: '8744'
abstract:
- lang: eng
text: Understanding the conformational sampling of translation-arrested ribosome
nascent chain complexes is key to understand co-translational folding. Up to now,
coupling of cysteine oxidation, disulfide bond formation and structure formation
in nascent chains has remained elusive. Here, we investigate the eye-lens protein
γB-crystallin in the ribosomal exit tunnel. Using mass spectrometry, theoretical
simulations, dynamic nuclear polarization-enhanced solid-state nuclear magnetic
resonance and cryo-electron microscopy, we show that thiol groups of cysteine
residues undergo S-glutathionylation and S-nitrosylation and form non-native disulfide
bonds. Thus, covalent modification chemistry occurs already prior to nascent chain
release as the ribosome exit tunnel provides sufficient space even for disulfide
bond formation which can guide protein folding.
acknowledgement: 'We acknowledge help from Anja Seybert, Margot Frangakis, Diana Grewe,
Mikhail Eltsov, Utz Ermel, and Shintaro Aibara. The work was supported by Deutsche
Forschungsgemeinschaft in the CLiC graduate school. Work at the Center for Biomolecular
Magnetic Resonance (BMRZ) is supported by the German state of Hesse. The work at
BMRZ has been supported by the state of Hesse. L.S. has been supported by the DFG
graduate college: CLiC.'
article_number: '5569'
article_processing_charge: No
article_type: original
author:
- first_name: Linda
full_name: Schulte, Linda
last_name: Schulte
- first_name: Jiafei
full_name: Mao, Jiafei
last_name: Mao
- first_name: Julian
full_name: Reitz, Julian
last_name: Reitz
- first_name: Sridhar
full_name: Sreeramulu, Sridhar
last_name: Sreeramulu
- first_name: Denis
full_name: Kudlinzki, Denis
last_name: Kudlinzki
- first_name: Victor-Valentin
full_name: Hodirnau, Victor-Valentin
id: 3661B498-F248-11E8-B48F-1D18A9856A87
last_name: Hodirnau
- first_name: Jakob
full_name: Meier-Credo, Jakob
last_name: Meier-Credo
- first_name: Krishna
full_name: Saxena, Krishna
last_name: Saxena
- first_name: Florian
full_name: Buhr, Florian
last_name: Buhr
- first_name: Julian D.
full_name: Langer, Julian D.
last_name: Langer
- first_name: Martin
full_name: Blackledge, Martin
last_name: Blackledge
- first_name: Achilleas S.
full_name: Frangakis, Achilleas S.
last_name: Frangakis
- first_name: Clemens
full_name: Glaubitz, Clemens
last_name: Glaubitz
- first_name: Harald
full_name: Schwalbe, Harald
last_name: Schwalbe
citation:
ama: Schulte L, Mao J, Reitz J, et al. Cysteine oxidation and disulfide formation
in the ribosomal exit tunnel. Nature Communications. 2020;11. doi:10.1038/s41467-020-19372-x
apa: Schulte, L., Mao, J., Reitz, J., Sreeramulu, S., Kudlinzki, D., Hodirnau, V.-V.,
… Schwalbe, H. (2020). Cysteine oxidation and disulfide formation in the ribosomal
exit tunnel. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-19372-x
chicago: Schulte, Linda, Jiafei Mao, Julian Reitz, Sridhar Sreeramulu, Denis Kudlinzki,
Victor-Valentin Hodirnau, Jakob Meier-Credo, et al. “Cysteine Oxidation and Disulfide
Formation in the Ribosomal Exit Tunnel.” Nature Communications. Springer
Nature, 2020. https://doi.org/10.1038/s41467-020-19372-x.
ieee: L. Schulte et al., “Cysteine oxidation and disulfide formation in the
ribosomal exit tunnel,” Nature Communications, vol. 11. Springer Nature,
2020.
ista: Schulte L, Mao J, Reitz J, Sreeramulu S, Kudlinzki D, Hodirnau V-V, Meier-Credo
J, Saxena K, Buhr F, Langer JD, Blackledge M, Frangakis AS, Glaubitz C, Schwalbe
H. 2020. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel.
Nature Communications. 11, 5569.
mla: Schulte, Linda, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal
Exit Tunnel.” Nature Communications, vol. 11, 5569, Springer Nature, 2020,
doi:10.1038/s41467-020-19372-x.
short: L. Schulte, J. Mao, J. Reitz, S. Sreeramulu, D. Kudlinzki, V.-V. Hodirnau,
J. Meier-Credo, K. Saxena, F. Buhr, J.D. Langer, M. Blackledge, A.S. Frangakis,
C. Glaubitz, H. Schwalbe, Nature Communications 11 (2020).
date_created: 2020-11-09T07:49:36Z
date_published: 2020-11-04T00:00:00Z
date_updated: 2023-08-22T12:36:07Z
day: '04'
ddc:
- '570'
department:
- _id: EM-Fac
doi: 10.1038/s41467-020-19372-x
external_id:
isi:
- '000592028600001'
file:
- access_level: open_access
checksum: b2688f0347e69e6629bba582077278c5
content_type: application/pdf
creator: dernst
date_created: 2020-11-09T07:56:24Z
date_updated: 2020-11-09T07:56:24Z
file_id: '8745'
file_name: 2020_NatureComm_Schulte.pdf
file_size: 1670898
relation: main_file
success: 1
file_date_updated: 2020-11-09T07:56:24Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
keyword:
- General Biochemistry
- Genetics and Molecular Biology
- General Physics and Astronomy
- General Chemistry
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cysteine oxidation and disulfide formation in the ribosomal exit tunnel
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '8787'
abstract:
- lang: eng
text: Breakdown of vascular barriers is a major complication of inflammatory diseases.
Anucleate platelets form blood-clots during thrombosis, but also play a crucial
role in inflammation. While spatio-temporal dynamics of clot formation are well
characterized, the cell-biological mechanisms of platelet recruitment to inflammatory
micro-environments remain incompletely understood. Here we identify Arp2/3-dependent
lamellipodia formation as a prominent morphological feature of immune-responsive
platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the
inflamed vasculature and to directionally spread, to polarize and to govern haptotactic
migration along gradients of the adhesive ligand. Platelet-specific abrogation
of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions,
thus impairing vascular sealing and provoking inflammatory microbleeding. During
infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination,
rendering platelets gate-keepers of the inflamed microvasculature. Consequently,
these findings identify haptotaxis as a key effector function of immune-responsive
platelets.
acknowledgement: "We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate
Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael
Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M.
[B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P.
[Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and
F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist
Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]),
FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.),
FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.),
and LMUexcellence NFF (F.G.). This project has received funding from the European
Research Council (ERC) under the European Union’s Horizon 2020 research and innovation
program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European
Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie
grant agreement no.\r\n747687."
article_number: '5778'
article_processing_charge: No
article_type: original
author:
- first_name: Leo
full_name: Nicolai, Leo
last_name: Nicolai
- first_name: Karin
full_name: Schiefelbein, Karin
last_name: Schiefelbein
- first_name: Silvia
full_name: Lipsky, Silvia
last_name: Lipsky
- first_name: Alexander
full_name: Leunig, Alexander
last_name: Leunig
- first_name: Marie
full_name: Hoffknecht, Marie
last_name: Hoffknecht
- first_name: Kami
full_name: Pekayvaz, Kami
last_name: Pekayvaz
- first_name: Ben
full_name: Raude, Ben
last_name: Raude
- first_name: Charlotte
full_name: Marx, Charlotte
last_name: Marx
- first_name: Andreas
full_name: Ehrlich, Andreas
last_name: Ehrlich
- first_name: Joachim
full_name: Pircher, Joachim
last_name: Pircher
- first_name: Zhe
full_name: Zhang, Zhe
last_name: Zhang
- first_name: Inas
full_name: Saleh, Inas
last_name: Saleh
- first_name: Anna-Kristina
full_name: Marel, Anna-Kristina
last_name: Marel
- first_name: Achim
full_name: Löf, Achim
last_name: Löf
- first_name: Tobias
full_name: Petzold, Tobias
last_name: Petzold
- first_name: Michael
full_name: Lorenz, Michael
last_name: Lorenz
- first_name: Konstantin
full_name: Stark, Konstantin
last_name: Stark
- first_name: Robert
full_name: Pick, Robert
last_name: Pick
- first_name: Gerhild
full_name: Rosenberger, Gerhild
last_name: Rosenberger
- first_name: Ludwig
full_name: Weckbach, Ludwig
last_name: Weckbach
- first_name: Bernd
full_name: Uhl, Bernd
last_name: Uhl
- first_name: Sheng
full_name: Xia, Sheng
last_name: Xia
- first_name: Christoph Andreas
full_name: Reichel, Christoph Andreas
last_name: Reichel
- first_name: Barbara
full_name: Walzog, Barbara
last_name: Walzog
- first_name: Christian
full_name: Schulz, Christian
last_name: Schulz
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Markus
full_name: Bender, Markus
last_name: Bender
- first_name: Rong
full_name: Li, Rong
last_name: Li
- first_name: Steffen
full_name: Massberg, Steffen
last_name: Massberg
- first_name: Florian R
full_name: Gärtner, Florian R
id: 397A88EE-F248-11E8-B48F-1D18A9856A87
last_name: Gärtner
orcid: 0000-0001-6120-3723
citation:
ama: Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic
blood platelets in inflammation and infection. Nature Communications. 2020;11.
doi:10.1038/s41467-020-19515-0
apa: Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz,
K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets
in inflammation and infection. Nature Communications. Springer Nature.
https://doi.org/10.1038/s41467-020-19515-0
chicago: Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie
Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic
Blood Platelets in Inflammation and Infection.” Nature Communications.
Springer Nature, 2020. https://doi.org/10.1038/s41467-020-19515-0.
ieee: L. Nicolai et al., “Vascular surveillance by haptotactic blood platelets
in inflammation and infection,” Nature Communications, vol. 11. Springer
Nature, 2020.
ista: Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude
B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T,
Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA,
Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular
surveillance by haptotactic blood platelets in inflammation and infection. Nature
Communications. 11, 5778.
mla: Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets
in Inflammation and Infection.” Nature Communications, vol. 11, 5778, Springer
Nature, 2020, doi:10.1038/s41467-020-19515-0.
short: L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz,
B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A.
Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B.
Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li,
S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).
date_created: 2020-11-22T23:01:23Z
date_published: 2020-11-13T00:00:00Z
date_updated: 2023-08-22T13:26:26Z
day: '13'
ddc:
- '570'
department:
- _id: MiSi
- _id: EM-Fac
doi: 10.1038/s41467-020-19515-0
ec_funded: 1
external_id:
isi:
- '000594648000014'
pmid:
- '33188196'
file:
- access_level: open_access
checksum: 485b7b6cf30198ba0ce126491a28f125
content_type: application/pdf
creator: dernst
date_created: 2020-11-23T13:29:49Z
date_updated: 2020-11-23T13:29:49Z
file_id: '8798'
file_name: 2020_NatureComm_Nicolai.pdf
file_size: 7035340
relation: main_file
success: 1
file_date_updated: 2020-11-23T13:29:49Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '747687'
name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Nature Communications
publication_identifier:
eissn:
- '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1038/s41467-022-31310-7
scopus_import: '1'
status: public
title: Vascular surveillance by haptotactic blood platelets in inflammation and infection
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '8971'
abstract:
- lang: eng
text: The actin-related protein (Arp)2/3 complex nucleates branched actin filament
networks pivotal for cell migration, endocytosis and pathogen infection. Its activation
is tightly regulated and involves complex structural rearrangements and actin
filament binding, which are yet to be understood. Here, we report a 9.0 Å resolution
structure of the actin filament Arp2/3 complex branch junction in cells using
cryo-electron tomography and subtomogram averaging. This allows us to generate
an accurate model of the active Arp2/3 complex in the branch junction and its
interaction with actin filaments. Notably, our model reveals a previously undescribed
set of interactions of the Arp2/3 complex with the mother filament, significantly
different to the previous branch junction model. Our structure also indicates
a central role for the ArpC3 subunit in stabilizing the active conformation.
acknowledged_ssus:
- _id: ScienComp
- _id: LifeSc
- _id: Bio
- _id: EM-Fac
acknowledgement: "This research was supported by the Scientific Service Units (SSUs)
of IST Austria through resources provided by Scientific Computing (SciComp), the
Life Science Facility (LSF), the BioImaging Facility (BIF), and the Electron Microscopy
Facility (EMF). We also thank Dimitry Tegunov (MPI for Biophysical Chemistry) for
helpful discussions\r\nabout the M software, and Michael Sixt (IST Austria) and
Klemens Rottner (Technical University Braunschweig, HZI Braunschweig) for critical
reading of the manuscript. We also thank Gregory Voth (University of Chicago) for
providing us the MD-derived branch junction model for comparison. The authors acknowledge
support from IST Austria and from the Austrian Science Fund (FWF): M02495 to G.D.
and Austrian Science Fund (FWF): P33367 to F.K.M.S. "
article_number: '6437'
article_processing_charge: No
article_type: original
author:
- first_name: Florian
full_name: Fäßler, Florian
id: 404F5528-F248-11E8-B48F-1D18A9856A87
last_name: Fäßler
orcid: 0000-0001-7149-769X
- first_name: Georgi A
full_name: Dimchev, Georgi A
id: 38C393BE-F248-11E8-B48F-1D18A9856A87
last_name: Dimchev
orcid: 0000-0001-8370-6161
- first_name: Victor-Valentin
full_name: Hodirnau, Victor-Valentin
id: 3661B498-F248-11E8-B48F-1D18A9856A87
last_name: Hodirnau
- first_name: William
full_name: Wan, William
last_name: Wan
- first_name: Florian KM
full_name: Schur, Florian KM
id: 48AD8942-F248-11E8-B48F-1D18A9856A87
last_name: Schur
orcid: 0000-0003-4790-8078
citation:
ama: Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. Cryo-electron tomography
structure of Arp2/3 complex in cells reveals new insights into the branch junction.
Nature Communications. 2020;11. doi:10.1038/s41467-020-20286-x
apa: Fäßler, F., Dimchev, G. A., Hodirnau, V.-V., Wan, W., & Schur, F. K. (2020).
Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights
into the branch junction. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-20286-x
chicago: Fäßler, Florian, Georgi A Dimchev, Victor-Valentin Hodirnau, William Wan,
and Florian KM Schur. “Cryo-Electron Tomography Structure of Arp2/3 Complex in
Cells Reveals New Insights into the Branch Junction.” Nature Communications.
Springer Nature, 2020. https://doi.org/10.1038/s41467-020-20286-x.
ieee: F. Fäßler, G. A. Dimchev, V.-V. Hodirnau, W. Wan, and F. K. Schur, “Cryo-electron
tomography structure of Arp2/3 complex in cells reveals new insights into the
branch junction,” Nature Communications, vol. 11. Springer Nature, 2020.
ista: Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. 2020. Cryo-electron tomography
structure of Arp2/3 complex in cells reveals new insights into the branch junction.
Nature Communications. 11, 6437.
mla: Fäßler, Florian, et al. “Cryo-Electron Tomography Structure of Arp2/3 Complex
in Cells Reveals New Insights into the Branch Junction.” Nature Communications,
vol. 11, 6437, Springer Nature, 2020, doi:10.1038/s41467-020-20286-x.
short: F. Fäßler, G.A. Dimchev, V.-V. Hodirnau, W. Wan, F.K. Schur, Nature Communications
11 (2020).
date_created: 2020-12-23T08:25:45Z
date_published: 2020-12-22T00:00:00Z
date_updated: 2023-08-24T11:01:50Z
day: '22'
ddc:
- '570'
department:
- _id: FlSc
- _id: EM-Fac
doi: 10.1038/s41467-020-20286-x
external_id:
isi:
- '000603078000003'
file:
- access_level: open_access
checksum: 55d43ea0061cc4027ba45e966e1db8cc
content_type: application/pdf
creator: dernst
date_created: 2020-12-28T08:16:10Z
date_updated: 2020-12-28T08:16:10Z
file_id: '8975'
file_name: 2020_NatureComm_Faessler.pdf
file_size: 3958727
relation: main_file
success: 1
file_date_updated: 2020-12-28T08:16:10Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
keyword:
- General Biochemistry
- Genetics and Molecular Biology
- General Physics and Astronomy
- General Chemistry
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: 9B954C5C-BA93-11EA-9121-9846C619BF3A
grant_number: P33367
name: Structure and isoform diversity of the Arp2/3 complex
- _id: 2674F658-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02495
name: Protein structure and function in filopodia across scales
publication: Nature Communications
publication_identifier:
issn:
- 2041-1723
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/cutting-edge-technology-reveals-structures-within-cells/
scopus_import: '1'
status: public
title: Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights
into the branch junction
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '10866'
abstract:
- lang: eng
text: Recent discoveries have shown that, when two layers of van der Waals (vdW)
materials are superimposed with a relative twist angle between them, the electronic
properties of the coupled system can be dramatically altered. Here, we demonstrate
that a similar concept can be extended to the optics realm, particularly to propagating
phonon polaritons–hybrid light-matter interactions. To do this, we fabricate stacks
composed of two twisted slabs of a vdW crystal (α-MoO3) supporting anisotropic
phonon polaritons (PhPs), and image the propagation of the latter when launched
by localized sources. Our images reveal that, under a critical angle, the PhPs
isofrequency curve undergoes a topological transition, in which the propagation
of PhPs is strongly guided (canalization regime) along predetermined directions
without geometric spreading. These results demonstrate a new degree of freedom
(twist angle) for controlling the propagation of polaritons at the nanoscale with
potential for nanoimaging, (bio)-sensing, or heat management.
acknowledgement: "J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa
Program from the\r\nGovernment of the Principality of Asturias (nos. PA-18-PF-BP17-126
and PA20-PF-BP19-053,\r\nrespectively). J. M-S acknowledges financial support through
the Ramón y Cajal Program from\r\nthe Government of Spain (RYC2018-026196-I). A.Y.N.
acknowledges the Spanish Ministry of\r\nScience, Innovation and Universities (national
project no. MAT201788358-C3-3-R). P.A.-G.\r\nacknowledges support from the European
Research Council under starting grant no. 715496,\r\n2DNANOPTICA."
article_processing_charge: No
article_type: original
author:
- first_name: Jiahua
full_name: Duan, Jiahua
last_name: Duan
- first_name: Nathaniel
full_name: Capote-Robayna, Nathaniel
last_name: Capote-Robayna
- first_name: Javier
full_name: Taboada-Gutiérrez, Javier
last_name: Taboada-Gutiérrez
- first_name: Gonzalo
full_name: Álvarez-Pérez, Gonzalo
last_name: Álvarez-Pérez
- first_name: Ivan
full_name: Prieto Gonzalez, Ivan
id: 2A307FE2-F248-11E8-B48F-1D18A9856A87
last_name: Prieto Gonzalez
orcid: 0000-0002-7370-5357
- first_name: Javier
full_name: Martín-Sánchez, Javier
last_name: Martín-Sánchez
- first_name: Alexey Y.
full_name: Nikitin, Alexey Y.
last_name: Nikitin
- first_name: Pablo
full_name: Alonso-González, Pablo
last_name: Alonso-González
citation:
ama: 'Duan J, Capote-Robayna N, Taboada-Gutiérrez J, et al. Twisted nano-optics:
Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano
Letters. 2020;20(7):5323-5329. doi:10.1021/acs.nanolett.0c01673'
apa: 'Duan, J., Capote-Robayna, N., Taboada-Gutiérrez, J., Álvarez-Pérez, G., Prieto
Gonzalez, I., Martín-Sánchez, J., … Alonso-González, P. (2020). Twisted nano-optics:
Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano
Letters. American Chemical Society. https://doi.org/10.1021/acs.nanolett.0c01673'
chicago: 'Duan, Jiahua, Nathaniel Capote-Robayna, Javier Taboada-Gutiérrez, Gonzalo
Álvarez-Pérez, Ivan Prieto Gonzalez, Javier Martín-Sánchez, Alexey Y. Nikitin,
and Pablo Alonso-González. “Twisted Nano-Optics: Manipulating Light at the Nanoscale
with Twisted Phonon Polaritonic Slabs.” Nano Letters. American Chemical
Society, 2020. https://doi.org/10.1021/acs.nanolett.0c01673.'
ieee: 'J. Duan et al., “Twisted nano-optics: Manipulating light at the nanoscale
with twisted phonon polaritonic slabs,” Nano Letters, vol. 20, no. 7. American
Chemical Society, pp. 5323–5329, 2020.'
ista: 'Duan J, Capote-Robayna N, Taboada-Gutiérrez J, Álvarez-Pérez G, Prieto Gonzalez
I, Martín-Sánchez J, Nikitin AY, Alonso-González P. 2020. Twisted nano-optics:
Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano
Letters. 20(7), 5323–5329.'
mla: 'Duan, Jiahua, et al. “Twisted Nano-Optics: Manipulating Light at the Nanoscale
with Twisted Phonon Polaritonic Slabs.” Nano Letters, vol. 20, no. 7, American
Chemical Society, 2020, pp. 5323–29, doi:10.1021/acs.nanolett.0c01673.'
short: J. Duan, N. Capote-Robayna, J. Taboada-Gutiérrez, G. Álvarez-Pérez, I. Prieto
Gonzalez, J. Martín-Sánchez, A.Y. Nikitin, P. Alonso-González, Nano Letters 20
(2020) 5323–5329.
date_created: 2022-03-18T11:37:38Z
date_published: 2020-07-01T00:00:00Z
date_updated: 2023-09-05T12:05:58Z
day: '01'
department:
- _id: NanoFab
doi: 10.1021/acs.nanolett.0c01673
external_id:
arxiv:
- '2004.14599'
isi:
- '000548893200082'
pmid:
- '32530634'
intvolume: ' 20'
isi: 1
issue: '7'
keyword:
- Mechanical Engineering
- Condensed Matter Physics
- General Materials Science
- General Chemistry
- Bioengineering
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/2004.14599
month: '07'
oa: 1
oa_version: Preprint
page: 5323-5329
pmid: 1
publication: Nano Letters
publication_identifier:
eissn:
- 1530-6992
issn:
- 1530-6984
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon
polaritonic slabs'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 20
year: '2020'
...
---
_id: '7687'
abstract:
- lang: eng
text: A working group, which was established within the Network of Repository Managers (RepManNet), has dealt with common certifications for repositories. In
addition, current requirements of the research funding agencies FWF and EU were
also taken into account. The Core Trust Seal was examined in more detail. For
this purpose, a questionnaire was sent to those organizations that are already certified
with CTS in Austria. The answers were summarized and evaluated anonymously. It
is recommended to go for a repository certification. Moreover, the development
of a DINI certificate in Austria is strongly suggested.
- lang: ger
text: ' Eine Arbeitsgruppe, die im Rahmen des Netzwerks für RepositorienmanagerInnen
(RepManNet) entstanden ist, hat sich mit gängigen Zertifizierungen für Repositorien
beschäftigt. Weiters wurden aktuelle Vorgaben der Forschungsförderer FWF und EU
herangezogen. Das Core Trust Seal wurde genauer betrachtet. Hierfür wurden jenen Organisationen, die in Österreich bereits mit CTS zertifiziert
sind, ein Fragebogen übermittelt. Die Antworten wurden anonymisiert zusammengefasst
und ausgewertet. Plädiert wird für eine Zertifizierung von Repositorien und die
Entwicklung einer DINI-Zertifizierung in Österreich.'
article_processing_charge: No
article_type: original
author:
- first_name: Doris
full_name: Ernst, Doris
id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
last_name: Ernst
orcid: 0000-0002-2354-0195
- first_name: Gertraud
full_name: Novotny, Gertraud
last_name: Novotny
- first_name: Eva Maria
full_name: Schönher, Eva Maria
last_name: Schönher
citation:
ama: Ernst D, Novotny G, Schönher EM. (Core Trust) Seal your repository! Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 2020;73(1):46-59.
doi:10.31263/voebm.v73i1.3491
apa: Ernst, D., Novotny, G., & Schönher, E. M. (2020). (Core Trust) Seal your
repository! Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen
und Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare.
https://doi.org/10.31263/voebm.v73i1.3491
chicago: Ernst, Doris, Gertraud Novotny, and Eva Maria Schönher. “(Core Trust) Seal
your repository!” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen
und Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare,
2020. https://doi.org/10.31263/voebm.v73i1.3491.
ieee: D. Ernst, G. Novotny, and E. M. Schönher, “(Core Trust) Seal your repository!,”
Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare,
vol. 73, no. 1. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare,
pp. 46–59, 2020.
ista: Ernst D, Novotny G, Schönher EM. 2020. (Core Trust) Seal your repository!
Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare.
73(1), 46–59.
mla: Ernst, Doris, et al. “(Core Trust) Seal your repository!” Mitteilungen der
Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol. 73,
no. 1, Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, 2020,
pp. 46–59, doi:10.31263/voebm.v73i1.3491.
short: D. Ernst, G. Novotny, E.M. Schönher, Mitteilungen der Vereinigung Österreichischer
Bibliothekarinnen und Bibliothekare 73 (2020) 46–59.
date_created: 2020-04-28T08:37:38Z
date_published: 2020-04-28T00:00:00Z
date_updated: 2024-03-12T10:12:33Z
day: '28'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.31263/voebm.v73i1.3491
file:
- access_level: open_access
checksum: fee784f15a489deb7def6ccf8c5bf8c3
content_type: application/pdf
creator: dernst
date_created: 2020-06-17T10:50:13Z
date_updated: 2024-03-12T10:12:33Z
file_id: '7970'
file_name: 2020_VOEB_Ernst.pdf
file_size: 579291
relation: main_file
file_date_updated: 2024-03-12T10:12:33Z
has_accepted_license: '1'
intvolume: ' 73'
issue: '1'
language:
- iso: ger
month: '04'
oa: 1
oa_version: Published Version
page: 46-59
popular_science: '1'
publication: Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare
publication_identifier:
issn:
- 1022-2588
publication_status: published
publisher: Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare
scopus_import: '1'
status: public
title: (Core Trust) Seal your repository!
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 73
year: '2020'
...
---
_id: '7800'
abstract:
- lang: eng
text: De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3
(CUL3) lead to autism spectrum disorder (ASD). Here, we used Cul3 mouse models
to evaluate the consequences of Cul3 mutations in vivo. Our results show that
Cul3 haploinsufficient mice exhibit deficits in motor coordination as well as
ASD-relevant social and cognitive impairments. Cul3 mutant brain displays cortical
lamination abnormalities due to defective neuronal migration and reduced numbers
of excitatory and inhibitory neurons. In line with the observed abnormal columnar
organization, Cul3 haploinsufficiency is associated with decreased spontaneous
excitatory and inhibitory activity in the cortex. At the molecular level, employing
a quantitative proteomic approach, we show that Cul3 regulates cytoskeletal and
adhesion protein abundance in mouse embryos. Abnormal regulation of cytoskeletal
proteins in Cul3 mutant neuronal cells results in atypical organization of the
actin mesh at the cell leading edge, likely causing the observed migration deficits.
In contrast to these important functions early in development, Cul3 deficiency
appears less relevant at adult stages. In fact, induction of Cul3 haploinsufficiency
in adult mice does not result in the behavioral defects observed in constitutive
Cul3 haploinsufficient animals. Taken together, our data indicate that Cul3 has
a critical role in the regulation of cytoskeletal proteins and neuronal migration
and that ASD-associated defects and behavioral abnormalities are primarily due
to Cul3 functions at early developmental stages.
acknowledged_ssus:
- _id: PreCl
article_processing_charge: No
author:
- first_name: Jasmin
full_name: Morandell, Jasmin
id: 4739D480-F248-11E8-B48F-1D18A9856A87
last_name: Morandell
- first_name: Lena A
full_name: Schwarz, Lena A
id: 29A8453C-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Bernadette
full_name: Basilico, Bernadette
id: 36035796-5ACA-11E9-A75E-7AF2E5697425
last_name: Basilico
orcid: 0000-0003-1843-3173
- first_name: Saren
full_name: Tasciyan, Saren
id: 4323B49C-F248-11E8-B48F-1D18A9856A87
last_name: Tasciyan
orcid: 0000-0003-1671-393X
- first_name: Armel
full_name: Nicolas, Armel
id: 2A103192-F248-11E8-B48F-1D18A9856A87
last_name: Nicolas
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Caroline
full_name: Kreuzinger, Caroline
id: 382077BA-F248-11E8-B48F-1D18A9856A87
last_name: Kreuzinger
- first_name: Lisa
full_name: Knaus, Lisa
id: 3B2ABCF4-F248-11E8-B48F-1D18A9856A87
last_name: Knaus
- first_name: Zoe
full_name: Dobler, Zoe
id: D23090A2-9057-11EA-883A-A8396FC7A38F
last_name: Dobler
- first_name: Emanuele
full_name: Cacci, Emanuele
last_name: Cacci
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
citation:
ama: Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein
homeostasis and cell migration during a critical window of brain development.
bioRxiv. doi:10.1101/2020.01.10.902064
apa: Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Nicolas, A., Sommer,
C. M., … Novarino, G. (n.d.). Cul3 regulates cytoskeleton protein homeostasis
and cell migration during a critical window of brain development. bioRxiv.
Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.01.10.902064
chicago: Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan,
Armel Nicolas, Christoph M Sommer, Caroline Kreuzinger, et al. “Cul3 Regulates
Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of
Brain Development.” BioRxiv. Cold Spring Harbor Laboratory, n.d. https://doi.org/10.1101/2020.01.10.902064 .
ieee: J. Morandell et al., “Cul3 regulates cytoskeleton protein homeostasis
and cell migration during a critical window of brain development,” bioRxiv.
Cold Spring Harbor Laboratory.
ista: Morandell J, Schwarz LA, Basilico B, Tasciyan S, Nicolas A, Sommer CM, Kreuzinger
C, Knaus L, Dobler Z, Cacci E, Danzl JG, Novarino G. Cul3 regulates cytoskeleton
protein homeostasis and cell migration during a critical window of brain development.
bioRxiv, 10.1101/2020.01.10.902064
.
mla: Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis
and Cell Migration during a Critical Window of Brain Development.” BioRxiv,
Cold Spring Harbor Laboratory, doi:10.1101/2020.01.10.902064 .
short: J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, A. Nicolas, C.M. Sommer,
C. Kreuzinger, L. Knaus, Z. Dobler, E. Cacci, J.G. Danzl, G. Novarino, BioRxiv
(n.d.).
date_created: 2020-05-05T14:31:33Z
date_published: 2020-01-11T00:00:00Z
date_updated: 2024-03-28T23:30:14Z
day: '11'
ddc:
- '570'
department:
- _id: JoDa
- _id: GaNo
- _id: LifeSc
doi: '10.1101/2020.01.10.902064 '
file:
- access_level: open_access
checksum: c6799ab5daba80efe8e2ed63c15f8c81
content_type: application/pdf
creator: rsix
date_created: 2020-05-05T14:31:19Z
date_updated: 2020-07-14T12:48:03Z
file_id: '7801'
file_name: 2020.01.10.902064v1.full.pdf
file_size: 2931370
relation: main_file
file_date_updated: 2020-07-14T12:48:03Z
has_accepted_license: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Preprint
project:
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
publication: bioRxiv
publication_status: submitted
publisher: Cold Spring Harbor Laboratory
related_material:
record:
- id: '9429'
relation: later_version
status: public
- id: '8620'
relation: dissertation_contains
status: public
status: public
title: Cul3 regulates cytoskeleton protein homeostasis and cell migration during a
critical window of brain development
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '9750'
abstract:
- lang: eng
text: Tension of the actomyosin cell cortex plays a key role in determining cell-cell
contact growth and size. The level of cortical tension outside of the cell-cell
contact, when pulling at the contact edge, scales with the total size to which
a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer
progenitor cells that this monotonic relationship only applies to a narrow range
of cortical tension increase, and that above a critical threshold, contact size
inversely scales with cortical tension. This switch from cortical tension increasing
to decreasing progenitor cell-cell contact size is caused by cortical tension
promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing
clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin
stabilization at the contact exceeds a critical threshold level, the rate by which
the contact expands in response to pulling forces from the cortex sharply drops,
leading to smaller contacts at physiologically relevant timescales of contact
formation. Thus, the activity of cortical tension in expanding cell-cell contact
size is limited by tension stabilizing E-cadherin-actin complexes at the contact.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: SSU
acknowledgement: We would like to thank Edouard Hannezo for discussions, Shayan Shami
Pour and Daniel Capek for help with data analysis, Vanessa Barone and other members
of the Heisenberg laboratory for thoughtful discussions and comments on the manuscript.
We also thank Jack Merrin for preparing the microwells, and the Scientific Service
Units at IST Austria, specifically Bioimaging and Electron Microscopy, and the Zebrafish
Facility for continuous support. We acknowledge Hitoshi Morita for the kind gift
of VinculinB-GFP plasmid. This research was supported by an ERC Advanced Grant (MECSPEC)
to C.-P.H, EMBO Long Term grant (ALTF 187-2013) to M.S and IST Fellow Marie-Curie
COFUND No. P_IST_EU01 to J.S.
article_processing_charge: No
author:
- first_name: Jana
full_name: Slovakova, Jana
id: 30F3F2F0-F248-11E8-B48F-1D18A9856A87
last_name: Slovakova
- first_name: Mateusz K
full_name: Sikora, Mateusz K
id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
last_name: Sikora
- first_name: Silvia
full_name: Caballero Mancebo, Silvia
id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
last_name: Caballero Mancebo
orcid: 0000-0002-5223-3346
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Karla
full_name: Huljev, Karla
id: 44C6F6A6-F248-11E8-B48F-1D18A9856A87
last_name: Huljev
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Slovakova J, Sikora MK, Caballero Mancebo S, et al. Tension-dependent stabilization
of E-cadherin limits cell-cell contact expansion. bioRxiv. 2020. doi:10.1101/2020.11.20.391284
apa: Slovakova, J., Sikora, M. K., Caballero Mancebo, S., Krens, G., Kaufmann, W.,
Huljev, K., & Heisenberg, C.-P. J. (2020). Tension-dependent stabilization
of E-cadherin limits cell-cell contact expansion. bioRxiv. Cold Spring
Harbor Laboratory. https://doi.org/10.1101/2020.11.20.391284
chicago: Slovakova, Jana, Mateusz K Sikora, Silvia Caballero Mancebo, Gabriel Krens,
Walter Kaufmann, Karla Huljev, and Carl-Philipp J Heisenberg. “Tension-Dependent
Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv.
Cold Spring Harbor Laboratory, 2020. https://doi.org/10.1101/2020.11.20.391284.
ieee: J. Slovakova et al., “Tension-dependent stabilization of E-cadherin
limits cell-cell contact expansion,” bioRxiv. Cold Spring Harbor Laboratory,
2020.
ista: Slovakova J, Sikora MK, Caballero Mancebo S, Krens G, Kaufmann W, Huljev K,
Heisenberg C-PJ. 2020. Tension-dependent stabilization of E-cadherin limits cell-cell
contact expansion. bioRxiv, 10.1101/2020.11.20.391284.
mla: Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits
Cell-Cell Contact Expansion.” BioRxiv, Cold Spring Harbor Laboratory, 2020,
doi:10.1101/2020.11.20.391284.
short: J. Slovakova, M.K. Sikora, S. Caballero Mancebo, G. Krens, W. Kaufmann, K.
Huljev, C.-P.J. Heisenberg, BioRxiv (2020).
date_created: 2021-07-29T11:29:50Z
date_published: 2020-11-20T00:00:00Z
date_updated: 2024-03-28T23:30:19Z
day: '20'
department:
- _id: CaHe
- _id: EM-Fac
- _id: Bio
doi: 10.1101/2020.11.20.391284
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2020.11.20.391284
month: '11'
oa: 1
oa_version: Preprint
page: '41'
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 2521E28E-B435-11E9-9278-68D0E5697425
grant_number: 187-2013
name: Modulation of adhesion function in cell-cell contact formation by cortical
tension
publication: bioRxiv
publication_status: published
publisher: Cold Spring Harbor Laboratory
related_material:
record:
- id: '10766'
relation: later_version
status: public
- id: '9623'
relation: dissertation_contains
status: public
status: public
title: Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion
type: preprint
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2020'
...
---
_id: '7885'
abstract:
- lang: eng
text: Eukaryotic cells migrate by coupling the intracellular force of the actin
cytoskeleton to the environment. While force coupling is usually mediated by transmembrane
adhesion receptors, especially those of the integrin family, amoeboid cells such
as leukocytes can migrate extremely fast despite very low adhesive forces1. Here
we show that leukocytes cannot only migrate under low adhesion but can also transmit
forces in the complete absence of transmembrane force coupling. When confined
within three-dimensional environments, they use the topographical features of
the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton
follows the texture of the substrate, creating retrograde shear forces that are
sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent
migration are not mutually exclusive, but rather are variants of the same principle
of coupling retrograde actin flow to the environment and thus can potentially
operate interchangeably and simultaneously. As adhesion-free migration is independent
of the chemical composition of the environment, it renders cells completely autonomous
in their locomotive behaviour.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
- _id: M-Shop
acknowledgement: We thank A. Leithner and J. Renkawitz for discussion and critical
reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic
setups; the Bioimaging Facility of IST Austria for excellent support, as well as
the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan,
L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme.
This work was supported by the European Research Council (ERC StG 281556 and CoG
724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF
to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476).
F.G. received funding from the European Union’s Horizon 2020 research and innovation
programme under the Marie Skłodowska-Curie grant agreement no. 747687.
article_processing_charge: No
article_type: original
author:
- first_name: Anne
full_name: Reversat, Anne
id: 35B76592-F248-11E8-B48F-1D18A9856A87
last_name: Reversat
orcid: 0000-0003-0666-8928
- first_name: Florian R
full_name: Gärtner, Florian R
id: 397A88EE-F248-11E8-B48F-1D18A9856A87
last_name: Gärtner
orcid: 0000-0001-6120-3723
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Julian A
full_name: Stopp, Julian A
id: 489E3F00-F248-11E8-B48F-1D18A9856A87
last_name: Stopp
- first_name: Saren
full_name: Tasciyan, Saren
id: 4323B49C-F248-11E8-B48F-1D18A9856A87
last_name: Tasciyan
orcid: 0000-0003-1671-393X
- first_name: Juan L
full_name: Aguilera Servin, Juan L
id: 2A67C376-F248-11E8-B48F-1D18A9856A87
last_name: Aguilera Servin
orcid: 0000-0002-2862-8372
- first_name: Ingrid
full_name: De Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: De Vries
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Miroslav
full_name: Hons, Miroslav
id: 4167FE56-F248-11E8-B48F-1D18A9856A87
last_name: Hons
orcid: 0000-0002-6625-3348
- first_name: Matthieu
full_name: Piel, Matthieu
last_name: Piel
- first_name: Andrew
full_name: Callan-Jones, Andrew
last_name: Callan-Jones
- first_name: Raphael
full_name: Voituriez, Raphael
last_name: Voituriez
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental
topography. Nature. 2020;582:582–585. doi:10.1038/s41586-020-2283-z
apa: Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera
Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography.
Nature. Springer Nature. https://doi.org/10.1038/s41586-020-2283-z
chicago: Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan,
Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental
Topography.” Nature. Springer Nature, 2020. https://doi.org/10.1038/s41586-020-2283-z.
ieee: A. Reversat et al., “Cellular locomotion using environmental topography,”
Nature, vol. 582. Springer Nature, pp. 582–585, 2020.
ista: Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL,
de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK.
2020. Cellular locomotion using environmental topography. Nature. 582, 582–585.
mla: Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.”
Nature, vol. 582, Springer Nature, 2020, pp. 582–585, doi:10.1038/s41586-020-2283-z.
short: A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera
Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez,
M.K. Sixt, Nature 582 (2020) 582–585.
date_created: 2020-05-24T22:01:01Z
date_published: 2020-06-25T00:00:00Z
date_updated: 2024-03-28T23:30:24Z
day: '25'
department:
- _id: NanoFab
- _id: Bio
- _id: MiSi
doi: 10.1038/s41586-020-2283-z
ec_funded: 1
external_id:
isi:
- '000532688300008'
intvolume: ' 582'
isi: 1
language:
- iso: eng
month: '06'
oa_version: None
page: 582–585
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '724373'
name: Cellular navigation along spatial gradients
- _id: 26018E70-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29911
name: Mechanical adaptation of lamellipodial actin
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '747687'
name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Nature
publication_identifier:
eissn:
- '14764687'
issn:
- '00280836'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/
record:
- id: '14697'
relation: dissertation_contains
status: public
- id: '12401'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Cellular locomotion using environmental topography
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 582
year: '2020'
...
---
_id: '8139'
abstract:
- lang: eng
text: 'Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated
in many aspects of plant growth, development, intra- and inter-cellular signaling,
nutrient uptake and pathogen defense. Despite these significant roles, little
is known about the precise molecular details of how it functions in planta. In
order to facilitate the direct quantitative study of plant CME, here we review
current routinely used methods and present refined, standardized quantitative
imaging protocols which allow the detailed characterization of CME at multiple
scales in plant tissues. These include: (i) an efficient electron microscopy protocol
for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for
the detailed characterization of the ultra-structure of clathrin-coated vesicles;
(ii) a detailed protocol and analysis for quantitative live-cell fluorescence
microscopy to precisely examine the temporal interplay of endocytosis components
during single CME events; (iii) a semi-automated analysis to allow the quantitative
characterization of global internalization of cargos in whole plant tissues; and
(iv) an overview and validation of useful genetic and pharmacological tools to
interrogate the molecular mechanisms and function of CME in intact plant samples.'
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
acknowledgement: "This paper is dedicated to the memory of Christien Merrifield. He
pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship
inspired the development of all\r\nthe analysis methods presented here. His joy
in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant
inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP
plants used in this manuscript. We further thank the\r\nScientific Service Units
at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical
assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility
for access to equipment. "
article_number: jcs248062
article_processing_charge: No
article_type: original
author:
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Nataliia
full_name: Gnyliukh, Nataliia
id: 390C1120-F248-11E8-B48F-1D18A9856A87
last_name: Gnyliukh
orcid: 0000-0002-2198-0509
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
- first_name: G
full_name: Vert, G
last_name: Vert
- first_name: SY
full_name: Bednarek, SY
last_name: Bednarek
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative
evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal
of Cell Science. 2020;133(15). doi:10.1242/jcs.248062
apa: Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek,
S., & Friml, J. (2020). Experimental toolbox for quantitative evaluation of
clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell
Science. The Company of Biologists. https://doi.org/10.1242/jcs.248062
chicago: Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan,
G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.248062.
ieee: A. J. Johnson et al., “Experimental toolbox for quantitative evaluation
of clathrin-mediated endocytosis in the plant model Arabidopsis,” Journal of
Cell Science, vol. 133, no. 15. The Company of Biologists, 2020.
ista: Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml
J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated
endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15),
jcs248062.
mla: Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation
of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of
Cell Science, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020,
doi:10.1242/jcs.248062.
short: A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek,
J. Friml, Journal of Cell Science 133 (2020).
date_created: 2020-07-21T08:58:19Z
date_published: 2020-08-06T00:00:00Z
date_updated: 2023-12-01T13:51:07Z
day: '06'
ddc:
- '575'
department:
- _id: JiFr
- _id: EM-Fac
doi: 10.1242/jcs.248062
ec_funded: 1
external_id:
isi:
- '000561047900021'
pmid:
- '32616560'
file:
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checksum: 2d11f79a0b4e0a380fb002b933da331a
content_type: application/pdf
creator: ajohnson
date_created: 2020-11-26T17:12:51Z
date_updated: 2021-08-08T22:30:03Z
embargo: 2021-08-07
file_id: '8815'
file_name: 2020 - Johnson - JSC - plant CME toolbox.pdf
file_size: 15150403
relation: main_file
file_date_updated: 2021-08-08T22:30:03Z
has_accepted_license: '1'
intvolume: ' 133'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: Journal of Cell Science
publication_identifier:
eissn:
- 1477-9137
issn:
- 0021-9533
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
related_material:
record:
- id: '14510'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis
in the plant model Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 133
year: '2020'
...
---
_id: '6819'
abstract:
- lang: eng
text: Glyphosate (N-phosphonomethyl glycine) and its commercial herbicide formulations
have been shown to exert toxicity via various mechanisms. It has been asserted
that glyphosate substitutes for glycine in polypeptide chains leading to protein
misfolding and toxicity. However, as no direct evidence exists for glycine to
glyphosate substitution in proteins, including in mammalian organisms, we tested
this claim by conducting a proteomics analysis of MDA-MB-231 human breast cancer
cells grown in the presence of 100 mg/L glyphosate for 6 days. Protein extracts
from three treated and three untreated cell cultures were analysed as one TMT-6plex
labelled sample, to highlight a specific pattern (+/+/+/−/−/−) of reporter intensities
for peptides bearing true glyphosate treatment induced-post translational modifications
as well as allowing an investigation of the total proteome.
article_number: '494'
article_processing_charge: No
author:
- first_name: Michael N.
full_name: Antoniou, Michael N.
last_name: Antoniou
- first_name: Armel
full_name: Nicolas, Armel
id: 2A103192-F248-11E8-B48F-1D18A9856A87
last_name: Nicolas
- first_name: Robin
full_name: Mesnage, Robin
last_name: Mesnage
- first_name: Martina
full_name: Biserni, Martina
last_name: Biserni
- first_name: Francesco V.
full_name: Rao, Francesco V.
last_name: Rao
- first_name: Cristina Vazquez
full_name: Martin, Cristina Vazquez
last_name: Martin
citation:
ama: Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. Glyphosate
does not substitute for glycine in proteins of actively dividing mammalian cells.
BMC Research Notes. 2019;12. doi:10.1186/s13104-019-4534-3
apa: Antoniou, M. N., Nicolas, A., Mesnage, R., Biserni, M., Rao, F. V., & Martin,
C. V. (2019). Glyphosate does not substitute for glycine in proteins of actively
dividing mammalian cells. BMC Research Notes. BioMed Central. https://doi.org/10.1186/s13104-019-4534-3
chicago: Antoniou, Michael N., Armel Nicolas, Robin Mesnage, Martina Biserni, Francesco
V. Rao, and Cristina Vazquez Martin. “Glyphosate Does Not Substitute for Glycine
in Proteins of Actively Dividing Mammalian Cells.” BMC Research Notes.
BioMed Central, 2019. https://doi.org/10.1186/s13104-019-4534-3.
ieee: M. N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F. V. Rao, and C. V. Martin,
“Glyphosate does not substitute for glycine in proteins of actively dividing mammalian
cells,” BMC Research Notes, vol. 12. BioMed Central, 2019.
ista: Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. 2019. Glyphosate
does not substitute for glycine in proteins of actively dividing mammalian cells.
BMC Research Notes. 12, 494.
mla: Antoniou, Michael N., et al. “Glyphosate Does Not Substitute for Glycine in
Proteins of Actively Dividing Mammalian Cells.” BMC Research Notes, vol.
12, 494, BioMed Central, 2019, doi:10.1186/s13104-019-4534-3.
short: M.N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F.V. Rao, C.V. Martin,
BMC Research Notes 12 (2019).
date_created: 2019-08-18T22:00:39Z
date_published: 2019-08-08T00:00:00Z
date_updated: 2023-02-23T14:08:14Z
day: '08'
ddc:
- '570'
department:
- _id: LifeSc
doi: 10.1186/s13104-019-4534-3
external_id:
pmid:
- '31395095'
file:
- access_level: open_access
checksum: 4a2bb7994b7f2c432bf44f5127ea3102
content_type: application/pdf
creator: dernst
date_created: 2019-08-23T11:10:35Z
date_updated: 2020-07-14T12:47:40Z
file_id: '6829'
file_name: 2019_BMC_Antoniou.pdf
file_size: 1177482
relation: main_file
file_date_updated: 2020-07-14T12:47:40Z
has_accepted_license: '1'
intvolume: ' 12'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: BMC Research Notes
publication_identifier:
eissn:
- 1756-0500
publication_status: published
publisher: BioMed Central
quality_controlled: '1'
related_material:
record:
- id: '9784'
relation: research_data
status: public
scopus_import: 1
status: public
title: Glyphosate does not substitute for glycine in proteins of actively dividing
mammalian cells
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2019'
...
---
_id: '9784'
abstract:
- lang: eng
text: 'Additional file 1: Table S1. Kinetics of MDA-MB-231 cell growth in either
the presence or absence of 100Â mg/L glyphosate. Cell counts are given at day-1
of seeding flasks and following 6-days of continuous culture. Note: no differences
in cell numbers were observed between negative control and glyphosate treated
cultures.'
article_processing_charge: No
author:
- first_name: Michael N.
full_name: Antoniou, Michael N.
last_name: Antoniou
- first_name: Armel
full_name: Nicolas, Armel
id: 2A103192-F248-11E8-B48F-1D18A9856A87
last_name: Nicolas
- first_name: Robin
full_name: Mesnage, Robin
last_name: Mesnage
- first_name: Martina
full_name: Biserni, Martina
last_name: Biserni
- first_name: Francesco V.
full_name: Rao, Francesco V.
last_name: Rao
- first_name: Cristina Vazquez
full_name: Martin, Cristina Vazquez
last_name: Martin
citation:
ama: Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. MOESM1 of
Glyphosate does not substitute for glycine in proteins of actively dividing mammalian
cells. 2019. doi:10.6084/m9.figshare.9411761.v1
apa: Antoniou, M. N., Nicolas, A., Mesnage, R., Biserni, M., Rao, F. V., & Martin,
C. V. (2019). MOESM1 of Glyphosate does not substitute for glycine in proteins
of actively dividing mammalian cells. Springer Nature. https://doi.org/10.6084/m9.figshare.9411761.v1
chicago: Antoniou, Michael N., Armel Nicolas, Robin Mesnage, Martina Biserni, Francesco
V. Rao, and Cristina Vazquez Martin. “MOESM1 of Glyphosate Does Not Substitute
for Glycine in Proteins of Actively Dividing Mammalian Cells.” Springer Nature,
2019. https://doi.org/10.6084/m9.figshare.9411761.v1.
ieee: M. N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F. V. Rao, and C. V. Martin,
“MOESM1 of Glyphosate does not substitute for glycine in proteins of actively
dividing mammalian cells.” Springer Nature, 2019.
ista: Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. 2019. MOESM1
of Glyphosate does not substitute for glycine in proteins of actively dividing
mammalian cells, Springer Nature, 10.6084/m9.figshare.9411761.v1.
mla: Antoniou, Michael N., et al. MOESM1 of Glyphosate Does Not Substitute for
Glycine in Proteins of Actively Dividing Mammalian Cells. Springer Nature,
2019, doi:10.6084/m9.figshare.9411761.v1.
short: M.N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F.V. Rao, C.V. Martin,
(2019).
date_created: 2021-08-06T08:14:05Z
date_published: 2019-08-09T00:00:00Z
date_updated: 2023-02-23T12:52:29Z
day: '09'
department:
- _id: LifeSc
doi: 10.6084/m9.figshare.9411761.v1
main_file_link:
- open_access: '1'
url: https://doi.org/10.6084/m9.figshare.9411761.v1
month: '08'
oa: 1
oa_version: Published Version
publisher: Springer Nature
related_material:
record:
- id: '6819'
relation: used_in_publication
status: public
status: public
title: MOESM1 of Glyphosate does not substitute for glycine in proteins of actively
dividing mammalian cells
type: research_data_reference
user_id: 6785fbc1-c503-11eb-8a32-93094b40e1cf
year: '2019'
...
---
_id: '12901'
article_processing_charge: No
author:
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: Janos
full_name: Kiss, Janos
id: 3D3A06F8-F248-11E8-B48F-1D18A9856A87
last_name: Kiss
- first_name: Stefano
full_name: Elefante, Stefano
id: 490F40CE-F248-11E8-B48F-1D18A9856A87
last_name: Elefante
citation:
ama: 'Schlögl A, Kiss J, Elefante S. Is Debian suitable for running an HPC Cluster?
In: AHPC19 - Austrian HPC Meeting 2019 . Institut für Mathematik und wissenschaftliches
Rechnen der Universität Graz; 2019:25.'
apa: 'Schlögl, A., Kiss, J., & Elefante, S. (2019). Is Debian suitable for running
an HPC Cluster? In AHPC19 - Austrian HPC Meeting 2019 (p. 25). Grundlsee,
Austria: Institut für Mathematik und wissenschaftliches Rechnen der Universität
Graz.'
chicago: Schlögl, Alois, Janos Kiss, and Stefano Elefante. “Is Debian Suitable for
Running an HPC Cluster?” In AHPC19 - Austrian HPC Meeting 2019 , 25. Institut
für Mathematik und wissenschaftliches Rechnen der Universität Graz, 2019.
ieee: A. Schlögl, J. Kiss, and S. Elefante, “Is Debian suitable for running an HPC
Cluster?,” in AHPC19 - Austrian HPC Meeting 2019 , Grundlsee, Austria,
2019, p. 25.
ista: 'Schlögl A, Kiss J, Elefante S. 2019. Is Debian suitable for running an HPC
Cluster? AHPC19 - Austrian HPC Meeting 2019 . AHPC: Austrian HPC Meeting, 25.'
mla: Schlögl, Alois, et al. “Is Debian Suitable for Running an HPC Cluster?” AHPC19
- Austrian HPC Meeting 2019 , Institut für Mathematik und wissenschaftliches
Rechnen der Universität Graz, 2019, p. 25.
short: A. Schlögl, J. Kiss, S. Elefante, in:, AHPC19 - Austrian HPC Meeting 2019
, Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz,
2019, p. 25.
conference:
end_date: 2019-02-27
location: Grundlsee, Austria
name: 'AHPC: Austrian HPC Meeting'
start_date: 2019-02-25
date_created: 2023-05-05T12:48:48Z
date_published: 2019-02-27T00:00:00Z
date_updated: 2023-05-16T07:29:32Z
day: '27'
ddc:
- '000'
department:
- _id: ScienComp
file:
- access_level: open_access
checksum: acc8272027faaf30709c51ac5c58ffa4
content_type: application/pdf
creator: dernst
date_created: 2023-05-16T07:27:09Z
date_updated: 2023-05-16T07:27:09Z
file_id: '12970'
file_name: 2019_AHPC_Schloegl.pdf
file_size: 1097603
relation: main_file
success: 1
file_date_updated: 2023-05-16T07:27:09Z
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc19/BOOKLET_AHPC19.pdf
month: '02'
oa: 1
oa_version: Published Version
page: '25'
publication: 'AHPC19 - Austrian HPC Meeting 2019 '
publication_status: published
publisher: Institut für Mathematik und wissenschaftliches Rechnen der Universität
Graz
status: public
title: Is Debian suitable for running an HPC Cluster?
type: conference_abstract
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2019'
...
---
_id: '6052'
abstract:
- lang: eng
text: 'Expansion microscopy is a relatively new approach to super-resolution imaging
that uses expandable hydrogels to isotropically increase the physical distance
between fluorophores in biological samples such as cell cultures or tissue slices.
The classic gel recipe results in an expansion factor of ~4×, with a resolution
of 60–80 nm. We have recently developed X10 microscopy, which uses a gel that
achieves an expansion factor of ~10×, with a resolution of ~25 nm. Here, we provide
a step-by-step protocol for X10 expansion microscopy. A typical experiment consists
of seven sequential stages: (i) immunostaining, (ii) anchoring, (iii) polymerization,
(iv) homogenization, (v) expansion, (vi) imaging, and (vii) validation. The protocol
presented here includes recommendations for optimization, pitfalls and their solutions,
and detailed guidelines that should increase reproducibility. Although our protocol
focuses on X10 expansion microscopy, we detail which of these suggestions are
also applicable to classic fourfold expansion microscopy. We exemplify our protocol
using primary hippocampal neurons from rats, but our approach can be used with
other primary cells or cultured cell lines of interest. This protocol will enable
any researcher with basic experience in immunostainings and access to an epifluorescence
microscope to perform super-resolution microscopy with X10. The procedure takes
3 d and requires ~5 h of actively handling the sample for labeling and expansion,
and another ~3 h for imaging and analysis.'
article_processing_charge: No
article_type: original
author:
- first_name: Sven M
full_name: Truckenbrodt, Sven M
id: 45812BD4-F248-11E8-B48F-1D18A9856A87
last_name: Truckenbrodt
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Silvio O
full_name: Rizzoli, Silvio O
last_name: Rizzoli
- first_name: Johann G
full_name: Danzl, Johann G
id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
last_name: Danzl
orcid: 0000-0001-8559-3973
citation:
ama: Truckenbrodt SM, Sommer CM, Rizzoli SO, Danzl JG. A practical guide to optimization
in X10 expansion microscopy. Nature Protocols. 2019;14(3):832–863. doi:10.1038/s41596-018-0117-3
apa: Truckenbrodt, S. M., Sommer, C. M., Rizzoli, S. O., & Danzl, J. G. (2019).
A practical guide to optimization in X10 expansion microscopy. Nature Protocols.
Nature Publishing Group. https://doi.org/10.1038/s41596-018-0117-3
chicago: Truckenbrodt, Sven M, Christoph M Sommer, Silvio O Rizzoli, and Johann
G Danzl. “A Practical Guide to Optimization in X10 Expansion Microscopy.” Nature
Protocols. Nature Publishing Group, 2019. https://doi.org/10.1038/s41596-018-0117-3.
ieee: S. M. Truckenbrodt, C. M. Sommer, S. O. Rizzoli, and J. G. Danzl, “A practical
guide to optimization in X10 expansion microscopy,” Nature Protocols, vol.
14, no. 3. Nature Publishing Group, pp. 832–863, 2019.
ista: Truckenbrodt SM, Sommer CM, Rizzoli SO, Danzl JG. 2019. A practical guide
to optimization in X10 expansion microscopy. Nature Protocols. 14(3), 832–863.
mla: Truckenbrodt, Sven M., et al. “A Practical Guide to Optimization in X10 Expansion
Microscopy.” Nature Protocols, vol. 14, no. 3, Nature Publishing Group,
2019, pp. 832–863, doi:10.1038/s41596-018-0117-3.
short: S.M. Truckenbrodt, C.M. Sommer, S.O. Rizzoli, J.G. Danzl, Nature Protocols
14 (2019) 832–863.
date_created: 2019-02-24T22:59:20Z
date_published: 2019-03-01T00:00:00Z
date_updated: 2023-08-24T14:48:33Z
day: '01'
ddc:
- '570'
department:
- _id: JoDa
- _id: Bio
doi: 10.1038/s41596-018-0117-3
ec_funded: 1
external_id:
isi:
- '000459890700008'
pmid:
- '30778205'
file:
- access_level: open_access
checksum: 7efb9951e7ddf3e3dcc2fb92b859c623
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: kschuh
date_created: 2021-06-29T14:41:46Z
date_updated: 2021-06-29T14:41:46Z
file_id: '9619'
file_name: 181031_Truckenbrodt_ExM_NatProtoc.docx
file_size: 84478958
relation: main_file
success: 1
file_date_updated: 2021-06-29T14:41:46Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Submitted Version
page: 832–863
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
- _id: 265CB4D0-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03600
name: Optical control of synaptic function via adhesion molecules
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
quality_controlled: '1'
scopus_import: '1'
status: public
title: A practical guide to optimization in X10 expansion microscopy
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2019'
...
---
_id: '6087'
abstract:
- lang: eng
text: Cell fate specification by lateral inhibition typically involves contact signaling
through the Delta-Notch signaling pathway. However, whether this is the only signaling
mode mediating lateral inhibition remains unclear. Here we show that in zebrafish
oogenesis, a group of cells within the granulosa cell layer at the oocyte animal
pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei.
One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly
high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically
compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly,
relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear
TAZ accumulation in neighboring cells, eventually leading to MPC re-specification
from these cells. Conversely, MPC specification is defective in taz−/− follicles.
These findings uncover a novel mode of lateral inhibition in cell fate specification
based on mechanical signals controlling TAZ activity.
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: LifeSc
acknowledgement: We thank Roland Dosch, Makoto Furutani-Seiki, Brian Link, Mary Mullins,
and Masazumi Tada for providing transgenic and/or mutant zebrafish lines; Alexandra
Schauer, Shayan Shami-Pour, and the rest of the Heisenberg lab for technical assistance
and feedback on the manuscript; and the Bioimaging, Electron Microscopy, and Zebrafish
facilities of IST Austria for continuous support. This work was supported by an
ERC advanced grant ( MECSPEC to C.-P.H.).
article_processing_charge: No
article_type: original
author:
- first_name: Peng
full_name: Xia, Peng
id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
last_name: Xia
orcid: 0000-0002-5419-7756
- first_name: Daniel J
full_name: Gütl, Daniel J
id: 381929CE-F248-11E8-B48F-1D18A9856A87
last_name: Gütl
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. Lateral inhibition in cell specification
mediated by mechanical signals modulating TAZ activity. Cell. 2019;176(6):1379-1392.e14.
doi:10.1016/j.cell.2019.01.019
apa: Xia, P., Gütl, D. J., Zheden, V., & Heisenberg, C.-P. J. (2019). Lateral
inhibition in cell specification mediated by mechanical signals modulating TAZ
activity. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.01.019
chicago: Xia, Peng, Daniel J Gütl, Vanessa Zheden, and Carl-Philipp J Heisenberg.
“Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating
TAZ Activity.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.01.019.
ieee: P. Xia, D. J. Gütl, V. Zheden, and C.-P. J. Heisenberg, “Lateral inhibition
in cell specification mediated by mechanical signals modulating TAZ activity,”
Cell, vol. 176, no. 6. Elsevier, p. 1379–1392.e14, 2019.
ista: Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. 2019. Lateral inhibition in cell
specification mediated by mechanical signals modulating TAZ activity. Cell. 176(6),
1379–1392.e14.
mla: Xia, Peng, et al. “Lateral Inhibition in Cell Specification Mediated by Mechanical
Signals Modulating TAZ Activity.” Cell, vol. 176, no. 6, Elsevier, 2019,
p. 1379–1392.e14, doi:10.1016/j.cell.2019.01.019.
short: P. Xia, D.J. Gütl, V. Zheden, C.-P.J. Heisenberg, Cell 176 (2019) 1379–1392.e14.
date_created: 2019-03-10T22:59:19Z
date_published: 2019-03-07T00:00:00Z
date_updated: 2023-08-25T08:02:23Z
day: '07'
department:
- _id: CaHe
- _id: EM-Fac
doi: 10.1016/j.cell.2019.01.019
ec_funded: 1
external_id:
isi:
- '000460509600013'
pmid:
- '30773315'
intvolume: ' 176'
isi: 1
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.cell.2019.01.019
month: '03'
oa: 1
oa_version: Published Version
page: 1379-1392.e14
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/in-zebrafish-eggs-most-rapidly-growing-cell-inhibits-its-neighbours-through-mechanical-signals/
scopus_import: '1'
status: public
title: Lateral inhibition in cell specification mediated by mechanical signals modulating
TAZ activity
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 176
year: '2019'
...
---
_id: '6607'
abstract:
- lang: eng
text: Acute myeloid leukemia (AML) is a heterogeneous disease with respect to its
genetic and molecular basis and to patients´ outcome. Clinical, cytogenetic, and
mutational data are used to classify patients into risk groups with different
survival, however, within-group heterogeneity is still an issue. Here, we used
a robust likelihood-based survival modeling approach and publicly available gene
expression data to identify a minimal number of genes whose combined expression
values were prognostic of overall survival. The resulting gene expression signature
(4-GES) consisted of 4 genes (SOCS2, IL2RA, NPDC1, PHGDH), predicted patient survival
as an independent prognostic parameter in several cohorts of AML patients (total,
1272 patients), and further refined prognostication based on the European Leukemia
Net classification. An oncogenic role of the top scoring gene in this signature,
SOCS2, was investigated using MLL-AF9 and Flt3-ITD/NPM1c driven mouse models of
AML. SOCS2 promoted leukemogenesis as well as the abundance, quiescence, and activity
of AML stem cells. Overall, the 4-GES represents a highly discriminating prognostic
parameter in AML, whose clinical applicability is greatly enhanced by its small
number of genes. The newly established role of SOCS2 in leukemia aggressiveness
and stemness raises the possibility that the signature might even be exploitable
therapeutically.
article_number: '9139'
article_processing_charge: No
author:
- first_name: Chi Huu
full_name: Nguyen, Chi Huu
last_name: Nguyen
- first_name: Tobias
full_name: Glüxam, Tobias
last_name: Glüxam
- first_name: Angela
full_name: Schlerka, Angela
last_name: Schlerka
- first_name: Katharina
full_name: Bauer, Katharina
id: 2ED6B14C-F248-11E8-B48F-1D18A9856A87
last_name: Bauer
- first_name: Alexander M.
full_name: Grandits, Alexander M.
last_name: Grandits
- first_name: Hubert
full_name: Hackl, Hubert
last_name: Hackl
- first_name: Oliver
full_name: Dovey, Oliver
last_name: Dovey
- first_name: Sabine
full_name: Zöchbauer-Müller, Sabine
last_name: Zöchbauer-Müller
- first_name: Jonathan L.
full_name: Cooper, Jonathan L.
last_name: Cooper
- first_name: George S.
full_name: Vassiliou, George S.
last_name: Vassiliou
- first_name: Dagmar
full_name: Stoiber, Dagmar
last_name: Stoiber
- first_name: Rotraud
full_name: Wieser, Rotraud
last_name: Wieser
- first_name: Gerwin
full_name: Heller, Gerwin
last_name: Heller
citation:
ama: Nguyen CH, Glüxam T, Schlerka A, et al. SOCS2 is part of a highly prognostic
4-gene signature in AML and promotes disease aggressiveness. Scientific Reports.
2019;9(1). doi:10.1038/s41598-019-45579-0
apa: Nguyen, C. H., Glüxam, T., Schlerka, A., Bauer, K., Grandits, A. M., Hackl,
H., … Heller, G. (2019). SOCS2 is part of a highly prognostic 4-gene signature
in AML and promotes disease aggressiveness. Scientific Reports. Nature
Publishing Group. https://doi.org/10.1038/s41598-019-45579-0
chicago: Nguyen, Chi Huu, Tobias Glüxam, Angela Schlerka, Katharina Bauer, Alexander
M. Grandits, Hubert Hackl, Oliver Dovey, et al. “SOCS2 Is Part of a Highly Prognostic
4-Gene Signature in AML and Promotes Disease Aggressiveness.” Scientific Reports.
Nature Publishing Group, 2019. https://doi.org/10.1038/s41598-019-45579-0.
ieee: C. H. Nguyen et al., “SOCS2 is part of a highly prognostic 4-gene signature
in AML and promotes disease aggressiveness,” Scientific Reports, vol. 9,
no. 1. Nature Publishing Group, 2019.
ista: Nguyen CH, Glüxam T, Schlerka A, Bauer K, Grandits AM, Hackl H, Dovey O, Zöchbauer-Müller
S, Cooper JL, Vassiliou GS, Stoiber D, Wieser R, Heller G. 2019. SOCS2 is part
of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness.
Scientific Reports. 9(1), 9139.
mla: Nguyen, Chi Huu, et al. “SOCS2 Is Part of a Highly Prognostic 4-Gene Signature
in AML and Promotes Disease Aggressiveness.” Scientific Reports, vol. 9,
no. 1, 9139, Nature Publishing Group, 2019, doi:10.1038/s41598-019-45579-0.
short: C.H. Nguyen, T. Glüxam, A. Schlerka, K. Bauer, A.M. Grandits, H. Hackl, O.
Dovey, S. Zöchbauer-Müller, J.L. Cooper, G.S. Vassiliou, D. Stoiber, R. Wieser,
G. Heller, Scientific Reports 9 (2019).
date_created: 2019-07-07T21:59:19Z
date_published: 2019-06-24T00:00:00Z
date_updated: 2023-08-28T12:26:51Z
day: '24'
ddc:
- '576'
department:
- _id: PreCl
doi: 10.1038/s41598-019-45579-0
external_id:
isi:
- '000472597400042'
file:
- access_level: open_access
checksum: 3283522fffadf4b5fc8c7adfe3ba4564
content_type: application/pdf
creator: kschuh
date_created: 2019-07-08T15:15:28Z
date_updated: 2020-07-14T12:47:34Z
file_id: '6623'
file_name: nature_2019_Nguyen.pdf
file_size: 2017352
relation: main_file
file_date_updated: 2020-07-14T12:47:34Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
quality_controlled: '1'
scopus_import: '1'
status: public
title: SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease
aggressiveness
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2019'
...
---
_id: '6867'
abstract:
- lang: eng
text: A novel magnetic scratch method achieves repeatability, reproducibility and
geometric control greater than pipette scratch assays and closely approximating
the precision of cell exclusion assays while inducing the cell injury inherently
necessary for wound healing assays. The magnetic scratch is affordable, easily
implemented and standardisable and thus may contribute toward better comparability
of data generated in different studies and laboratories.
article_number: '12625'
article_processing_charge: No
author:
- first_name: M.
full_name: Fenu, M.
last_name: Fenu
- first_name: T.
full_name: Bettermann, T.
last_name: Bettermann
- first_name: C.
full_name: Vogl, C.
last_name: Vogl
- first_name: Nasser
full_name: Darwish-Miranda, Nasser
id: 39CD9926-F248-11E8-B48F-1D18A9856A87
last_name: Darwish-Miranda
orcid: 0000-0002-8821-8236
- first_name: J.
full_name: Schramel, J.
last_name: Schramel
- first_name: F.
full_name: Jenner, F.
last_name: Jenner
- first_name: I.
full_name: Ribitsch, I.
last_name: Ribitsch
citation:
ama: Fenu M, Bettermann T, Vogl C, et al. A novel magnet-based scratch method for
standardisation of wound-healing assays. Scientific Reports. 2019;9(1).
doi:10.1038/s41598-019-48930-7
apa: Fenu, M., Bettermann, T., Vogl, C., Darwish-Miranda, N., Schramel, J., Jenner,
F., & Ribitsch, I. (2019). A novel magnet-based scratch method for standardisation
of wound-healing assays. Scientific Reports. Springer Nature. https://doi.org/10.1038/s41598-019-48930-7
chicago: Fenu, M., T. Bettermann, C. Vogl, Nasser Darwish-Miranda, J. Schramel,
F. Jenner, and I. Ribitsch. “A Novel Magnet-Based Scratch Method for Standardisation
of Wound-Healing Assays.” Scientific Reports. Springer Nature, 2019. https://doi.org/10.1038/s41598-019-48930-7.
ieee: M. Fenu et al., “A novel magnet-based scratch method for standardisation
of wound-healing assays,” Scientific Reports, vol. 9, no. 1. Springer Nature,
2019.
ista: Fenu M, Bettermann T, Vogl C, Darwish-Miranda N, Schramel J, Jenner F, Ribitsch
I. 2019. A novel magnet-based scratch method for standardisation of wound-healing
assays. Scientific Reports. 9(1), 12625.
mla: Fenu, M., et al. “A Novel Magnet-Based Scratch Method for Standardisation of
Wound-Healing Assays.” Scientific Reports, vol. 9, no. 1, 12625, Springer
Nature, 2019, doi:10.1038/s41598-019-48930-7.
short: M. Fenu, T. Bettermann, C. Vogl, N. Darwish-Miranda, J. Schramel, F. Jenner,
I. Ribitsch, Scientific Reports 9 (2019).
date_created: 2019-09-15T22:00:42Z
date_published: 2019-09-02T00:00:00Z
date_updated: 2023-08-29T07:55:15Z
day: '02'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1038/s41598-019-48930-7
external_id:
isi:
- '000483697800007'
pmid:
- '31477739'
file:
- access_level: open_access
checksum: 9cfd986d4108e288cc72276ef047ab0c
content_type: application/pdf
creator: dernst
date_created: 2019-09-16T12:42:40Z
date_updated: 2020-07-14T12:47:42Z
file_id: '6879'
file_name: 2019_ScientificReports_Fenu.pdf
file_size: 3523795
relation: main_file
file_date_updated: 2020-07-14T12:47:42Z
has_accepted_license: '1'
intvolume: ' 9'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Scientific Reports
publication_identifier:
eissn:
- '20452322'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: A novel magnet-based scratch method for standardisation of wound-healing assays
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 9
year: '2019'
...
---
_id: '7225'
abstract:
- lang: eng
text: "This is a literature teaching resource review for biologically inspired microfluidics
courses\r\nor exploring the diverse applications of microfluidics. The structure
is around key papers and model\r\norganisms. While courses gradually change over
time, a focus remains on understanding how\r\nmicrofluidics has developed as well
as what it can and cannot do for researchers. As a primary\r\nstarting point,
we cover micro-fluid mechanics principles and microfabrication of devices. A variety\r\nof
applications are discussed using model prokaryotic and eukaryotic organisms from
the set\r\nof bacteria (Escherichia coli), trypanosomes (Trypanosoma brucei),
yeast (Saccharomyces cerevisiae),\r\nslime molds (Physarum polycephalum), worms
(Caenorhabditis elegans), flies (Drosophila melangoster),\r\nplants (Arabidopsis
thaliana), and mouse immune cells (Mus musculus). Other engineering and\r\nbiochemical
methods discussed include biomimetics, organ on a chip, inkjet, droplet microfluidics,\r\nbiotic
games, and diagnostics. While we have not yet reached the end-all lab on a chip,\r\nmicrofluidics
can still be used effectively for specific applications."
article_number: '109'
article_processing_charge: Yes
article_type: review
author:
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
citation:
ama: Merrin J. Frontiers in microfluidics, a teaching resource review. Bioengineering.
2019;6(4). doi:10.3390/bioengineering6040109
apa: Merrin, J. (2019). Frontiers in microfluidics, a teaching resource review.
Bioengineering. MDPI. https://doi.org/10.3390/bioengineering6040109
chicago: Merrin, Jack. “Frontiers in Microfluidics, a Teaching Resource Review.”
Bioengineering. MDPI, 2019. https://doi.org/10.3390/bioengineering6040109.
ieee: J. Merrin, “Frontiers in microfluidics, a teaching resource review,” Bioengineering,
vol. 6, no. 4. MDPI, 2019.
ista: Merrin J. 2019. Frontiers in microfluidics, a teaching resource review. Bioengineering.
6(4), 109.
mla: Merrin, Jack. “Frontiers in Microfluidics, a Teaching Resource Review.” Bioengineering,
vol. 6, no. 4, 109, MDPI, 2019, doi:10.3390/bioengineering6040109.
short: J. Merrin, Bioengineering 6 (2019).
date_created: 2020-01-05T23:00:45Z
date_published: 2019-12-03T00:00:00Z
date_updated: 2023-09-06T14:52:49Z
day: '03'
ddc:
- '620'
department:
- _id: NanoFab
doi: 10.3390/bioengineering6040109
external_id:
isi:
- '000505590000024'
pmid:
- '31816954'
file:
- access_level: open_access
checksum: 80f1499e2a4caccdf3aa54b137fd99a0
content_type: application/pdf
creator: dernst
date_created: 2020-01-07T14:49:59Z
date_updated: 2020-07-14T12:47:54Z
file_id: '7243'
file_name: 2019_Bioengineering_Merrin.pdf
file_size: 2660780
relation: main_file
file_date_updated: 2020-07-14T12:47:54Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
issue: '4'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Bioengineering
publication_identifier:
eissn:
- '23065354'
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Frontiers in microfluidics, a teaching resource review
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 6
year: '2019'
...
---
_id: '7406'
abstract:
- lang: eng
text: "Background\r\nSynaptic vesicles (SVs) are an integral part of the neurotransmission
machinery, and isolation of SVs from their host neuron is necessary to reveal
their most fundamental biochemical and functional properties in in vitro assays.
Isolated SVs from neurons that have been genetically engineered, e.g. to introduce
genetically encoded indicators, are not readily available but would permit new
insights into SV structure and function. Furthermore, it is unclear if cultured
neurons can provide sufficient starting material for SV isolation procedures.\r\n\r\nNew
method\r\nHere, we demonstrate an efficient ex vivo procedure to obtain functional
SVs from cultured rat cortical neurons after genetic engineering with a lentivirus.\r\n\r\nResults\r\nWe
show that ∼108 plated cortical neurons allow isolation of suitable SV amounts
for functional analysis and imaging. We found that SVs isolated from cultured
neurons have neurotransmitter uptake comparable to that of SVs isolated from intact
cortex. Using total internal reflection fluorescence (TIRF) microscopy, we visualized
an exogenous SV-targeted marker protein and demonstrated the high efficiency of
SV modification.\r\n\r\nComparison with existing methods\r\nObtaining SVs from
genetically engineered neurons currently generally requires the availability of
transgenic animals, which is constrained by technical (e.g. cost and time) and
biological (e.g. developmental defects and lethality) limitations.\r\n\r\nConclusions\r\nThese
results demonstrate the modification and isolation of functional SVs using cultured
neurons and viral transduction. The ability to readily obtain SVs from genetically
engineered neurons will permit linking in situ studies to in vitro experiments
in a variety of genetic contexts."
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
article_processing_charge: No
article_type: original
author:
- first_name: Catherine
full_name: Mckenzie, Catherine
id: 3EEDE19A-F248-11E8-B48F-1D18A9856A87
last_name: Mckenzie
- first_name: Miroslava
full_name: Spanova, Miroslava
id: 44A924DC-F248-11E8-B48F-1D18A9856A87
last_name: Spanova
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Stephanie
full_name: Kainrath, Stephanie
id: 32CFBA64-F248-11E8-B48F-1D18A9856A87
last_name: Kainrath
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
orcid: 0000-0002-9438-4783
- first_name: Harald H.
full_name: Sitte, Harald H.
last_name: Sitte
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
citation:
ama: Mckenzie C, Spanova M, Johnson AJ, et al. Isolation of synaptic vesicles from
genetically engineered cultured neurons. Journal of Neuroscience Methods.
2019;312:114-121. doi:10.1016/j.jneumeth.2018.11.018
apa: Mckenzie, C., Spanova, M., Johnson, A. J., Kainrath, S., Zheden, V., Sitte,
H. H., & Janovjak, H. L. (2019). Isolation of synaptic vesicles from genetically
engineered cultured neurons. Journal of Neuroscience Methods. Elsevier.
https://doi.org/10.1016/j.jneumeth.2018.11.018
chicago: Mckenzie, Catherine, Miroslava Spanova, Alexander J Johnson, Stephanie
Kainrath, Vanessa Zheden, Harald H. Sitte, and Harald L Janovjak. “Isolation of
Synaptic Vesicles from Genetically Engineered Cultured Neurons.” Journal of
Neuroscience Methods. Elsevier, 2019. https://doi.org/10.1016/j.jneumeth.2018.11.018.
ieee: C. Mckenzie et al., “Isolation of synaptic vesicles from genetically
engineered cultured neurons,” Journal of Neuroscience Methods, vol. 312.
Elsevier, pp. 114–121, 2019.
ista: Mckenzie C, Spanova M, Johnson AJ, Kainrath S, Zheden V, Sitte HH, Janovjak
HL. 2019. Isolation of synaptic vesicles from genetically engineered cultured
neurons. Journal of Neuroscience Methods. 312, 114–121.
mla: Mckenzie, Catherine, et al. “Isolation of Synaptic Vesicles from Genetically
Engineered Cultured Neurons.” Journal of Neuroscience Methods, vol. 312,
Elsevier, 2019, pp. 114–21, doi:10.1016/j.jneumeth.2018.11.018.
short: C. Mckenzie, M. Spanova, A.J. Johnson, S. Kainrath, V. Zheden, H.H. Sitte,
H.L. Janovjak, Journal of Neuroscience Methods 312 (2019) 114–121.
date_created: 2020-01-30T09:12:19Z
date_published: 2019-01-15T00:00:00Z
date_updated: 2023-09-06T15:27:29Z
day: '15'
department:
- _id: HaJa
- _id: Bio
doi: 10.1016/j.jneumeth.2018.11.018
ec_funded: 1
external_id:
isi:
- '000456220900013'
pmid:
- '30496761'
intvolume: ' 312'
isi: 1
language:
- iso: eng
month: '01'
oa_version: None
page: 114-121
pmid: 1
project:
- _id: 25548C20-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '303564'
name: Microbial Ion Channels for Synthetic Neurobiology
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2548AE96-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W1232-B24
name: Molecular Drug Targets
publication: Journal of Neuroscience Methods
publication_identifier:
issn:
- 0165-0270
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Isolation of synaptic vesicles from genetically engineered cultured neurons
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 312
year: '2019'
...
---
_id: '7415'
article_processing_charge: No
article_type: original
author:
- first_name: Jasmin
full_name: Morandell, Jasmin
id: 4739D480-F248-11E8-B48F-1D18A9856A87
last_name: Morandell
- first_name: Armel
full_name: Nicolas, Armel
id: 2A103192-F248-11E8-B48F-1D18A9856A87
last_name: Nicolas
- first_name: Lena A
full_name: Schwarz, Lena A
id: 29A8453C-F248-11E8-B48F-1D18A9856A87
last_name: Schwarz
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
citation:
ama: Morandell J, Nicolas A, Schwarz LA, Novarino G. S.16.05 Illuminating the role
of the e3 ubiquitin ligase cullin3 in brain development and autism. European
Neuropsychopharmacology. 2019;29(Supplement 6):S11-S12. doi:10.1016/j.euroneuro.2019.09.040
apa: Morandell, J., Nicolas, A., Schwarz, L. A., & Novarino, G. (2019). S.16.05
Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development
and autism. European Neuropsychopharmacology. Elsevier. https://doi.org/10.1016/j.euroneuro.2019.09.040
chicago: Morandell, Jasmin, Armel Nicolas, Lena A Schwarz, and Gaia Novarino. “S.16.05
Illuminating the Role of the E3 Ubiquitin Ligase Cullin3 in Brain Development
and Autism.” European Neuropsychopharmacology. Elsevier, 2019. https://doi.org/10.1016/j.euroneuro.2019.09.040.
ieee: J. Morandell, A. Nicolas, L. A. Schwarz, and G. Novarino, “S.16.05 Illuminating
the role of the e3 ubiquitin ligase cullin3 in brain development and autism,”
European Neuropsychopharmacology, vol. 29, no. Supplement 6. Elsevier,
pp. S11–S12, 2019.
ista: Morandell J, Nicolas A, Schwarz LA, Novarino G. 2019. S.16.05 Illuminating
the role of the e3 ubiquitin ligase cullin3 in brain development and autism. European
Neuropsychopharmacology. 29(Supplement 6), S11–S12.
mla: Morandell, Jasmin, et al. “S.16.05 Illuminating the Role of the E3 Ubiquitin
Ligase Cullin3 in Brain Development and Autism.” European Neuropsychopharmacology,
vol. 29, no. Supplement 6, Elsevier, 2019, pp. S11–12, doi:10.1016/j.euroneuro.2019.09.040.
short: J. Morandell, A. Nicolas, L.A. Schwarz, G. Novarino, European Neuropsychopharmacology
29 (2019) S11–S12.
date_created: 2020-01-30T10:07:41Z
date_published: 2019-12-13T00:00:00Z
date_updated: 2023-09-07T14:56:17Z
day: '13'
department:
- _id: GaNo
- _id: LifeSc
doi: 10.1016/j.euroneuro.2019.09.040
external_id:
isi:
- '000502657500021'
intvolume: ' 29'
isi: 1
issue: Supplement 6
language:
- iso: eng
month: '12'
oa_version: None
page: S11-S12
publication: European Neuropsychopharmacology
publication_identifier:
issn:
- 0924-977X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: S.16.05 Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development
and autism
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 29
year: '2019'
...
---
_id: '6093'
abstract:
- lang: eng
text: Blebs are cellular protrusions observed in migrating cells and in cells undergoing
spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm
during bleb formation and the concurrent changes in cell volume using zebrafish
primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation
occurs concomitantly with cytoplasmic inflow into it and that during this process
the total cell volume does not change. We thus show that bleb formation in primordial
germ cells results primarily from redistribution of material within the cell rather
than being driven by flow of water from an external source.
article_number: e0212699
article_processing_charge: No
author:
- first_name: Mohammad
full_name: Goudarzi, Mohammad
id: 3384113A-F248-11E8-B48F-1D18A9856A87
last_name: Goudarzi
- first_name: Aleix
full_name: Boquet-Pujadas, Aleix
last_name: Boquet-Pujadas
- first_name: Jean Christophe
full_name: Olivo-Marin, Jean Christophe
last_name: Olivo-Marin
- first_name: Erez
full_name: Raz, Erez
last_name: Raz
citation:
ama: Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E. Fluid dynamics during
bleb formation in migrating cells in vivo. PLOS ONE. 2019;14(2). doi:10.1371/journal.pone.0212699
apa: Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J. C., & Raz, E. (2019).
Fluid dynamics during bleb formation in migrating cells in vivo. PLOS ONE.
Public Library of Science. https://doi.org/10.1371/journal.pone.0212699
chicago: Goudarzi, Mohammad, Aleix Boquet-Pujadas, Jean Christophe Olivo-Marin,
and Erez Raz. “Fluid Dynamics during Bleb Formation in Migrating Cells in Vivo.”
PLOS ONE. Public Library of Science, 2019. https://doi.org/10.1371/journal.pone.0212699.
ieee: M. Goudarzi, A. Boquet-Pujadas, J. C. Olivo-Marin, and E. Raz, “Fluid dynamics
during bleb formation in migrating cells in vivo,” PLOS ONE, vol. 14, no.
2. Public Library of Science, 2019.
ista: Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E. 2019. Fluid dynamics
during bleb formation in migrating cells in vivo. PLOS ONE. 14(2), e0212699.
mla: Goudarzi, Mohammad, et al. “Fluid Dynamics during Bleb Formation in Migrating
Cells in Vivo.” PLOS ONE, vol. 14, no. 2, e0212699, Public Library of Science,
2019, doi:10.1371/journal.pone.0212699.
short: M. Goudarzi, A. Boquet-Pujadas, J.C. Olivo-Marin, E. Raz, PLOS ONE 14 (2019).
date_created: 2019-03-10T22:59:21Z
date_published: 2019-02-26T00:00:00Z
date_updated: 2023-09-19T14:46:47Z
day: '26'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1371/journal.pone.0212699
external_id:
isi:
- '000459712100022'
file:
- access_level: open_access
checksum: b885de050ed4bb3c86f706487a47197f
content_type: application/pdf
creator: dernst
date_created: 2019-03-11T16:09:23Z
date_updated: 2020-07-14T12:47:19Z
file_id: '6096'
file_name: 2019_PLoSOne_Goudarzi.pdf
file_size: 2967731
relation: main_file
file_date_updated: 2020-07-14T12:47:19Z
has_accepted_license: '1'
intvolume: ' 14'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
publication: PLOS ONE
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fluid dynamics during bleb formation in migrating cells in vivo
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 14
year: '2019'
...
---
_id: '6657'
abstract:
- lang: eng
text: 'In this article a model is described how Open Access definitions can be formed
on the basis of objective criteria. The common Open Access definitions such as
"gold" and "green" are not exactly defined. This becomes a problem as soon as
one begins to measure Open Access, for example if the development of the Open
Access share should be monitored. This was discussed in the working group on Open
Access Monitoring of the AT2OA project and the present model was developed,
which is based on 5 critics with 4 characteristics: location, licence, version,
embargo and conditions of the Open Access publication are taken into account.
In the meantime, the model has also been tested in practice using R scripts, and
the initial results are quite promising.'
article_processing_charge: No
article_type: original
author:
- first_name: Patrick
full_name: Danowski, Patrick
id: 2EBD1598-F248-11E8-B48F-1D18A9856A87
last_name: Danowski
orcid: 0000-0002-6026-4409
citation:
ama: Danowski P. An Austrian proposal for the classification of Open Access Tuples
(COAT) - distinguish different open access types beyond colors. Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 2019;72(1):59-65.
doi:10.31263/voebm.v72i1.2276
apa: Danowski, P. (2019). An Austrian proposal for the classification of Open Access
Tuples (COAT) - distinguish different open access types beyond colors. Mitteilungen
Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare. Vereinigung
Österreichischer Bibliothekarinnen und Bibliothekare. https://doi.org/10.31263/voebm.v72i1.2276
chicago: Danowski, Patrick. “An Austrian Proposal for the Classification of Open
Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors.”
Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare.
Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2019. https://doi.org/10.31263/voebm.v72i1.2276.
ieee: P. Danowski, “An Austrian proposal for the classification of Open Access Tuples
(COAT) - distinguish different open access types beyond colors,” Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol.
72, no. 1. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, pp.
59–65, 2019.
ista: Danowski P. 2019. An Austrian proposal for the classification of Open Access
Tuples (COAT) - distinguish different open access types beyond colors. Mitteilungen
der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 72(1), 59–65.
mla: Danowski, Patrick. “An Austrian Proposal for the Classification of Open Access
Tuples (COAT) - Distinguish Different Open Access Types beyond Colors.” Mitteilungen
Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare, vol.
72, no. 1, Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2019,
pp. 59–65, doi:10.31263/voebm.v72i1.2276.
short: P. Danowski, Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen
Und Bibliothekare 72 (2019) 59–65.
date_created: 2019-07-21T21:59:15Z
date_published: 2019-05-17T00:00:00Z
date_updated: 2023-10-17T11:33:58Z
day: '17'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.31263/voebm.v72i1.2276
file:
- access_level: open_access
checksum: c0d2695d6d0d34e62ba06fb3f0ebaaed
content_type: application/pdf
creator: apreinsp
date_created: 2019-07-22T08:45:03Z
date_updated: 2020-07-14T12:47:35Z
file_id: '6661'
file_name: 2019_MitteilungenDerVOEB_Danowski.pdf
file_size: 468558
relation: main_file
file_date_updated: 2020-07-14T12:47:35Z
has_accepted_license: '1'
intvolume: ' 72'
issue: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 59-65
publication: Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare
publication_identifier:
eissn:
- 1022-2588
publication_status: published
publisher: Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare
quality_controlled: '1'
related_material:
record:
- id: '5686'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: An Austrian proposal for the classification of Open Access Tuples (COAT) -
distinguish different open access types beyond colors
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 72
year: '2019'
...
---
_id: '6328'
abstract:
- lang: eng
text: During metazoan development, immune surveillance and cancer dissemination,
cells migrate in complex three-dimensional microenvironments1,2,3. These spaces
are crowded by cells and extracellular matrix, generating mazes with differently
sized gaps that are typically smaller than the diameter of the migrating cell4,5.
Most mesenchymal and epithelial cells and some—but not all—cancer cells actively
generate their migratory path using pericellular tissue proteolysis6. By contrast,
amoeboid cells such as leukocytes use non-destructive strategies of locomotion7,
raising the question how these extremely fast cells navigate through dense tissues.
Here we reveal that leukocytes sample their immediate vicinity for large pore
sizes, and are thereby able to choose the path of least resistance. This allows
them to circumnavigate local obstacles while effectively following global directional
cues such as chemotactic gradients. Pore-size discrimination is facilitated by
frontward positioning of the nucleus, which enables the cells to use their bulkiest
compartment as a mechanical gauge. Once the nucleus and the closely associated
microtubule organizing centre pass the largest pore, cytoplasmic protrusions still
lingering in smaller pores are retracted. These retractions are coordinated by
dynamic microtubules; when microtubules are disrupted, migrating cells lose coherence
and frequently fragment into migratory cytoplasmic pieces. As nuclear positioning
in front of the microtubule organizing centre is a typical feature of amoeboid
migration, our findings link the fundamental organization of cellular polarity
to the strategy of locomotion.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: letter_note
author:
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
- first_name: Aglaja
full_name: Kopf, Aglaja
id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
last_name: Kopf
orcid: 0000-0002-2187-6656
- first_name: Julian A
full_name: Stopp, Julian A
id: 489E3F00-F248-11E8-B48F-1D18A9856A87
last_name: Stopp
- first_name: Ingrid
full_name: de Vries, Ingrid
id: 4C7D837E-F248-11E8-B48F-1D18A9856A87
last_name: de Vries
- first_name: Meghan K.
full_name: Driscoll, Meghan K.
last_name: Driscoll
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Erik S.
full_name: Welf, Erik S.
last_name: Welf
- first_name: Gaudenz
full_name: Danuser, Gaudenz
last_name: Danuser
- first_name: Reto
full_name: Fiolka, Reto
last_name: Fiolka
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Renkawitz J, Kopf A, Stopp JA, et al. Nuclear positioning facilitates amoeboid
migration along the path of least resistance. Nature. 2019;568:546-550.
doi:10.1038/s41586-019-1087-5
apa: Renkawitz, J., Kopf, A., Stopp, J. A., de Vries, I., Driscoll, M. K., Merrin,
J., … Sixt, M. K. (2019). Nuclear positioning facilitates amoeboid migration along
the path of least resistance. Nature. Springer Nature. https://doi.org/10.1038/s41586-019-1087-5
chicago: Renkawitz, Jörg, Aglaja Kopf, Julian A Stopp, Ingrid de Vries, Meghan K.
Driscoll, Jack Merrin, Robert Hauschild, et al. “Nuclear Positioning Facilitates
Amoeboid Migration along the Path of Least Resistance.” Nature. Springer
Nature, 2019. https://doi.org/10.1038/s41586-019-1087-5.
ieee: J. Renkawitz et al., “Nuclear positioning facilitates amoeboid migration
along the path of least resistance,” Nature, vol. 568. Springer Nature,
pp. 546–550, 2019.
ista: Renkawitz J, Kopf A, Stopp JA, de Vries I, Driscoll MK, Merrin J, Hauschild
R, Welf ES, Danuser G, Fiolka R, Sixt MK. 2019. Nuclear positioning facilitates
amoeboid migration along the path of least resistance. Nature. 568, 546–550.
mla: Renkawitz, Jörg, et al. “Nuclear Positioning Facilitates Amoeboid Migration
along the Path of Least Resistance.” Nature, vol. 568, Springer Nature,
2019, pp. 546–50, doi:10.1038/s41586-019-1087-5.
short: J. Renkawitz, A. Kopf, J.A. Stopp, I. de Vries, M.K. Driscoll, J. Merrin,
R. Hauschild, E.S. Welf, G. Danuser, R. Fiolka, M.K. Sixt, Nature 568 (2019) 546–550.
date_created: 2019-04-17T06:52:28Z
date_published: 2019-04-25T00:00:00Z
date_updated: 2024-03-28T23:30:40Z
day: '25'
department:
- _id: MiSi
- _id: NanoFab
- _id: Bio
doi: 10.1038/s41586-019-1087-5
ec_funded: 1
external_id:
isi:
- '000465594200050'
pmid:
- '30944468'
intvolume: ' 568'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217284/
month: '04'
oa: 1
oa_version: Submitted Version
page: 546-550
pmid: 1
project:
- _id: 25A603A2-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '281556'
name: Cytoskeletal force generation and force transduction of migrating leukocytes
(EU)
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '724373'
name: Cellular navigation along spatial gradients
- _id: 265FAEBA-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: W01250-B20
name: Nano-Analytics of Cellular Systems
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 25A48D24-B435-11E9-9278-68D0E5697425
grant_number: ALTF 1396-2014
name: Molecular and system level view of immune cell migration
publication: Nature
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/leukocytes-use-their-nucleus-as-a-ruler-to-choose-path-of-least-resistance/
record:
- id: '14697'
relation: dissertation_contains
status: public
- id: '6891'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Nuclear positioning facilitates amoeboid migration along the path of least
resistance
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 568
year: '2019'
...
---
_id: '53'
abstract:
- lang: eng
text: In 2013, a publication repository was implemented at IST Austria and 2015
after a thorough preparation phase a data repository was implemented - both based
on the Open Source Software EPrints. In this text, designed as field report, we
will reflect on our experiences with Open Source Software in general and specifically
with EPrints regarding technical aspects but also regarding their characteristics
of the user community. The second part is a pleading for including the end users
in the process of implementation, adaption and evaluation.
author:
- first_name: Barbara
full_name: Petritsch, Barbara
id: 406048EC-F248-11E8-B48F-1D18A9856A87
last_name: Petritsch
orcid: 0000-0003-2724-4614
- first_name: Jana
full_name: Porsche, Jana
id: 3252EDC2-F248-11E8-B48F-1D18A9856A87
last_name: Porsche
citation:
ama: 'Petritsch B, Porsche J. IST PubRep and IST DataRep: the institutional repositories
at IST Austria. VÖB Mitteilungen. 2018;71(1):199-206. doi:10.31263/voebm.v71i1.1993'
apa: 'Petritsch, B., & Porsche, J. (2018). IST PubRep and IST DataRep: the institutional
repositories at IST Austria. VÖB Mitteilungen. Vereinigung Österreichischer
Bibliothekarinnen und Bibliothekare. https://doi.org/10.31263/voebm.v71i1.1993'
chicago: 'Petritsch, Barbara, and Jana Porsche. “IST PubRep and IST DataRep: The
Institutional Repositories at IST Austria.” VÖB Mitteilungen. Vereinigung
Österreichischer Bibliothekarinnen und Bibliothekare, 2018. https://doi.org/10.31263/voebm.v71i1.1993.'
ieee: 'B. Petritsch and J. Porsche, “IST PubRep and IST DataRep: the institutional
repositories at IST Austria,” VÖB Mitteilungen, vol. 71, no. 1. Vereinigung
Österreichischer Bibliothekarinnen und Bibliothekare, pp. 199–206, 2018.'
ista: 'Petritsch B, Porsche J. 2018. IST PubRep and IST DataRep: the institutional
repositories at IST Austria. VÖB Mitteilungen. 71(1), 199–206.'
mla: 'Petritsch, Barbara, and Jana Porsche. “IST PubRep and IST DataRep: The Institutional
Repositories at IST Austria.” VÖB Mitteilungen, vol. 71, no. 1, Vereinigung
Österreichischer Bibliothekarinnen und Bibliothekare, 2018, pp. 199–206, doi:10.31263/voebm.v71i1.1993.'
short: B. Petritsch, J. Porsche, VÖB Mitteilungen 71 (2018) 199–206.
date_created: 2018-12-11T11:44:22Z
date_published: 2018-10-01T00:00:00Z
date_updated: 2021-01-12T08:01:26Z
day: '01'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.31263/voebm.v71i1.1993
file:
- access_level: open_access
checksum: 7ac61bade5f37db011ca435ebcf86797
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T12:40:27Z
date_updated: 2020-07-14T12:46:38Z
file_id: '5702'
file_name: 2018_VOEB_Petritsch.pdf
file_size: 509434
relation: main_file
file_date_updated: 2020-07-14T12:46:38Z
has_accepted_license: '1'
intvolume: ' 71'
issue: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 199 - 206
publication: VÖB Mitteilungen
publication_status: published
publisher: Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare
publist_id: '8001'
scopus_import: 1
status: public
title: 'IST PubRep and IST DataRep: the institutional repositories at IST Austria'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 71
year: '2018'
...
---
_id: '6459'
author:
- first_name: Barbara
full_name: Petritsch, Barbara
id: 406048EC-F248-11E8-B48F-1D18A9856A87
last_name: Petritsch
orcid: 0000-0003-2724-4614
citation:
ama: Petritsch B. Open Access at IST Austria 2009-2017. IST Austria; 2018.
doi:10.5281/zenodo.1410279
apa: 'Petritsch, B. (2018). Open Access at IST Austria 2009-2017. Presented
at the Open-Access-Tage, Graz, Austria: IST Austria. https://doi.org/10.5281/zenodo.1410279'
chicago: Petritsch, Barbara. Open Access at IST Austria 2009-2017. IST Austria,
2018. https://doi.org/10.5281/zenodo.1410279.
ieee: B. Petritsch, Open Access at IST Austria 2009-2017. IST Austria, 2018.
ista: Petritsch B. 2018. Open Access at IST Austria 2009-2017, IST Austria,p.
mla: Petritsch, Barbara. Open Access at IST Austria 2009-2017. IST Austria,
2018, doi:10.5281/zenodo.1410279.
short: B. Petritsch, Open Access at IST Austria 2009-2017, IST Austria, 2018.
conference:
end_date: 2018-09-26
location: Graz, Austria
name: Open-Access-Tage
start_date: 2018-09-24
date_created: 2019-05-16T07:27:14Z
date_published: 2018-09-24T00:00:00Z
date_updated: 2020-07-14T23:06:21Z
day: '24'
ddc:
- '020'
department:
- _id: E-Lib
doi: 10.5281/zenodo.1410279
file:
- access_level: open_access
checksum: 9063ab4d10ea93353c3a03bbf53fbcf1
content_type: application/pdf
creator: dernst
date_created: 2019-05-16T07:26:25Z
date_updated: 2020-07-14T12:47:30Z
file_id: '6460'
file_name: Poster_Beitrag_125_Petritsch.pdf
file_size: 1967778
relation: main_file
file_date_updated: 2020-07-14T12:47:30Z
has_accepted_license: '1'
keyword:
- Open Access
- Publication Analysis
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication_status: published
publisher: IST Austria
status: public
title: Open Access at IST Austria 2009-2017
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: conference_poster
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2018'
...
---
_id: '308'
abstract:
- lang: eng
text: Migrating cells penetrate tissue barriers during development, inflammatory
responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally
confined environments requires changes in the mechanical properties of the surrounding
cells using embryonic Drosophila melanogaster hemocytes, also called macrophages,
as a model. We find that macrophage invasion into the germband through transient
separation of the apposing ectoderm and mesoderm requires cell deformations and
reductions in apical tension in the ectoderm. Interestingly, the genetic pathway
governing these mechanical shifts acts downstream of the only known tumor necrosis
factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald.
Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal
cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated
tight junction protein). We therefore elucidate a distinct molecular pathway that
controls tissue tension and demonstrate the importance of such regulation for
invasive migration in vivo.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: original
author:
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Julia
full_name: Biebl, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Biebl
- first_name: Michael
full_name: Smutny, Michael
last_name: Smutny
- first_name: Jana
full_name: Veselá, Jana
id: 433253EE-F248-11E8-B48F-1D18A9856A87
last_name: Veselá
- first_name: Ekaterina
full_name: Papusheva, Ekaterina
id: 41DB591E-F248-11E8-B48F-1D18A9856A87
last_name: Papusheva
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Alessandra M
full_name: Casano, Alessandra M
id: 3DBA3F4E-F248-11E8-B48F-1D18A9856A87
last_name: Casano
orcid: 0000-0002-6009-6804
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension
in the embryo to facilitate macrophage invasive migration. Developmental Cell.
2018;45(3):331-346. doi:10.1016/j.devcel.2018.04.002
apa: Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G.,
… Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo
to facilitate macrophage invasive migration. Developmental Cell. Elsevier.
https://doi.org/10.1016/j.devcel.2018.04.002
chicago: Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina
Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano,
and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to
Facilitate Macrophage Invasive Migration.” Developmental Cell. Elsevier,
2018. https://doi.org/10.1016/j.devcel.2018.04.002.
ieee: A. Ratheesh et al., “Drosophila TNF modulates tissue tension in the
embryo to facilitate macrophage invasive migration,” Developmental Cell,
vol. 45, no. 3. Elsevier, pp. 331–346, 2018.
ista: Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W,
György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension
in the embryo to facilitate macrophage invasive migration. Developmental Cell.
45(3), 331–346.
mla: Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo
to Facilitate Macrophage Invasive Migration.” Developmental Cell, vol.
45, no. 3, Elsevier, 2018, pp. 331–46, doi:10.1016/j.devcel.2018.04.002.
short: A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W.
Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018)
331–346.
date_created: 2018-12-11T11:45:44Z
date_published: 2018-05-07T00:00:00Z
date_updated: 2023-09-11T13:22:13Z
day: '07'
department:
- _id: DaSi
- _id: CaHe
- _id: Bio
- _id: EM-Fac
- _id: MiSi
doi: 10.1016/j.devcel.2018.04.002
ec_funded: 1
external_id:
isi:
- '000432461400009'
pmid:
- '29738712'
intvolume: ' 45'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.devcel.2018.04.002
month: '05'
oa: 1
oa_version: Published Version
page: 331 - 346
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Developmental Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/
scopus_import: '1'
status: public
title: Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage
invasive migration
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 45
year: '2018'
...