---
_id: '8582'
abstract:
- lang: eng
text: "Cell and tissue polarization is fundamental for plant growth and morphogenesis.
The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial
for their function in directional auxin transport. The clustering of PIN polar
cargoes within the plasma membrane has been proposed to be important for the maintenance
of their polar distribution. However, the more detailed features of PIN clusters
and the cellular requirements of cargo clustering remain unclear.\r\nHere, we
characterized PIN clusters in detail by means of multiple advanced microscopy
and quantification methods, such as 3D quantitative imaging or freeze‐fracture
replica labeling. The size and aggregation types of PIN clusters were determined
by electron microscopy at the nanometer level at different polar domains and at
different developmental stages, revealing a strong preference for clustering at
the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters
depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall
components as well as connections between the cell wall and the plasma membrane.\r\nThis
study identifies the role of different cellular processes and structures in polar
cargo clustering and provides initial mechanistic insight into the maintenance
of polarity in plants and other systems."
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg)
for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1
mutant and the complementation lines, and Dr Patricia C. Zambryski (University of
California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria)
for assistance with imaging, group members for discussions, Martine De Cock for
help in preparing the manuscript and Nataliia Gnyliukh for critical reading and
revision of the manuscript. This project received funding from the European Research
Council (ERC) under the European Union's Horizon 2020 research and innovation program
(grant agreement No. 742985) and Comisión Nacional de Investigación Científica y
Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People
Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme
(FP7/2007‐2013) under REA grant agreement no. 291734.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Daniel
full_name: von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Nasser
full_name: Darwish-Miranda, Nasser
id: 39CD9926-F248-11E8-B48F-1D18A9856A87
last_name: Darwish-Miranda
orcid: 0000-0002-8821-8236
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Riet
full_name: de Rycke, Riet
last_name: de Rycke
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Daniel J
full_name: Gütl, Daniel J
id: 381929CE-F248-11E8-B48F-1D18A9856A87
last_name: Gütl
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Meiyu
full_name: Ke, Meiyu
last_name: Ke
- first_name: Xu
full_name: Chen, Xu
id: 4E5ADCAA-F248-11E8-B48F-1D18A9856A87
last_name: Chen
- first_name: Jan
full_name: Dettmer, Jan
last_name: Dettmer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar
cargo clustering in Arabidopsis thaliana. New Phytologist. 2021;229(1):351-369.
doi:10.1111/nph.16887
apa: Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto,
S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in
Arabidopsis thaliana. New Phytologist. Wiley. https://doi.org/10.1111/nph.16887
chicago: Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda,
Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar
Cargo Clustering in Arabidopsis Thaliana.” New Phytologist. Wiley, 2021.
https://doi.org/10.1111/nph.16887.
ieee: H. Li et al., “Cellular requirements for PIN polar cargo clustering
in Arabidopsis thaliana,” New Phytologist, vol. 229, no. 1. Wiley, pp.
351–369, 2021.
ista: Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik
KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer
J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis
thaliana. New Phytologist. 229(1), 351–369.
mla: Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering
in Arabidopsis Thaliana.” New Phytologist, vol. 229, no. 1, Wiley, 2021,
pp. 351–69, doi:10.1111/nph.16887.
short: H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto,
K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke,
X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.
date_created: 2020-09-28T08:59:28Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2023-08-04T11:01:21Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EM-Fac
- _id: Bio
- _id: EvBe
doi: 10.1111/nph.16887
ec_funded: 1
external_id:
isi:
- '000570187900001'
file:
- access_level: open_access
checksum: b45621607b4cab97eeb1605ab58e896e
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T09:44:17Z
date_updated: 2021-02-04T09:44:17Z
file_id: '9084'
file_name: 2021_NewPhytologist_Li.pdf
file_size: 4061962
relation: main_file
success: 1
file_date_updated: 2021-02-04T09:44:17Z
has_accepted_license: '1'
intvolume: ' 229'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 351-369
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: New Phytologist
publication_identifier:
eissn:
- '14698137'
issn:
- 0028646X
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 229
year: '2021'
...
---
_id: '8927'
abstract:
- lang: eng
text: The recent outbreak of coronavirus disease 2019 (COVID‐19), caused by the
Severe Acute Respiratory Syndrome Coronavirus‐2 (SARS‐CoV‐2) has resulted in a
world‐wide pandemic. Disseminated lung injury with the development of acute respiratory
distress syndrome (ARDS) is the main cause of mortality in COVID‐19. Although
liver failure does not seem to occur in the absence of pre‐existing liver disease,
hepatic involvement in COVID‐19 may correlate with overall disease severity and
serve as a prognostic factor for the development of ARDS. The spectrum of liver
injury in COVID‐19 may range from direct infection by SARS‐CoV‐2, indirect involvement
by systemic inflammation, hypoxic changes, iatrogenic causes such as drugs and
ventilation to exacerbation of underlying liver disease. This concise review discusses
the potential pathophysiological mechanisms for SARS‐CoV‐2 hepatic tropism as
well as acute and possibly long‐term liver injury in COVID‐19.
acknowledgement: This work was supported by grant F7310‐B21 from the Austrian Science
Foundation (to MT). We thank Jelena Remetic, Claudia D. Fuchs, Veronika Mlitz and
Daniel Steinacher, for their valuable input and discussion. Figure 1 and Figure
2 have been created with BioRender.com.
article_processing_charge: No
article_type: original
author:
- first_name: Alexander D.
full_name: Nardo, Alexander D.
last_name: Nardo
- first_name: Mathias
full_name: Schneeweiss-Gleixner, Mathias
last_name: Schneeweiss-Gleixner
- first_name: May M
full_name: Bakail, May M
id: FB3C3F8E-522F-11EA-B186-22963DDC885E
last_name: Bakail
orcid: 0000-0002-9592-1587
- first_name: Emmanuel D.
full_name: Dixon, Emmanuel D.
last_name: Dixon
- first_name: Sigurd F.
full_name: Lax, Sigurd F.
last_name: Lax
- first_name: Michael
full_name: Trauner, Michael
last_name: Trauner
citation:
ama: Nardo AD, Schneeweiss-Gleixner M, Bakail MM, Dixon ED, Lax SF, Trauner M. Pathophysiological
mechanisms of liver injury in COVID-19. Liver International. 2021;41(1):20-32.
doi:10.1111/liv.14730
apa: Nardo, A. D., Schneeweiss-Gleixner, M., Bakail, M. M., Dixon, E. D., Lax, S.
F., & Trauner, M. (2021). Pathophysiological mechanisms of liver injury in
COVID-19. Liver International. Wiley. https://doi.org/10.1111/liv.14730
chicago: Nardo, Alexander D., Mathias Schneeweiss-Gleixner, May M Bakail, Emmanuel
D. Dixon, Sigurd F. Lax, and Michael Trauner. “Pathophysiological Mechanisms of
Liver Injury in COVID-19.” Liver International. Wiley, 2021. https://doi.org/10.1111/liv.14730.
ieee: A. D. Nardo, M. Schneeweiss-Gleixner, M. M. Bakail, E. D. Dixon, S. F. Lax,
and M. Trauner, “Pathophysiological mechanisms of liver injury in COVID-19,” Liver
International, vol. 41, no. 1. Wiley, pp. 20–32, 2021.
ista: Nardo AD, Schneeweiss-Gleixner M, Bakail MM, Dixon ED, Lax SF, Trauner M.
2021. Pathophysiological mechanisms of liver injury in COVID-19. Liver International.
41(1), 20–32.
mla: Nardo, Alexander D., et al. “Pathophysiological Mechanisms of Liver Injury
in COVID-19.” Liver International, vol. 41, no. 1, Wiley, 2021, pp. 20–32,
doi:10.1111/liv.14730.
short: A.D. Nardo, M. Schneeweiss-Gleixner, M.M. Bakail, E.D. Dixon, S.F. Lax, M.
Trauner, Liver International 41 (2021) 20–32.
date_created: 2020-12-06T23:01:16Z
date_published: 2021-01-01T00:00:00Z
date_updated: 2023-08-04T11:19:51Z
day: '01'
ddc:
- '570'
department:
- _id: CampIT
doi: 10.1111/liv.14730
external_id:
isi:
- '000594239200001'
file:
- access_level: open_access
checksum: 6e4f21b77ef22c854e016240974fc473
content_type: application/pdf
creator: dernst
date_created: 2021-02-04T12:01:45Z
date_updated: 2021-02-04T12:01:45Z
file_id: '9091'
file_name: 2021_Liver_Nardo.pdf
file_size: 930414
relation: main_file
success: 1
file_date_updated: 2021-02-04T12:01:45Z
has_accepted_license: '1'
intvolume: ' 41'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 20-32
publication: Liver International
publication_identifier:
eissn:
- '14783231'
issn:
- '14783223'
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Pathophysiological mechanisms of liver injury in COVID-19
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 41
year: '2021'
...
---
_id: '9038'
abstract:
- lang: eng
text: 'Layered materials in which individual atomic layers are bonded by weak van
der Waals forces (vdW materials) constitute one of the most prominent platforms
for materials research. Particularly, polar vdW crystals, such as hexagonal boron
nitride (h-BN), alpha-molybdenum trioxide (α-MoO3) or alpha-vanadium pentoxide
(α-V2O5), have received significant attention in nano-optics, since they support
phonon polaritons (PhPs)―light coupled to lattice vibrations― with strong electromagnetic
confinement and low optical losses. Recently, correlative far- and near-field
studies of α-MoO3 have been demonstrated as an effective strategy to accurately
extract the permittivity of this material. Here, we use this accurately characterized
and low-loss polaritonic material to sense its local dielectric environment, namely
silica (SiO2), one of the most widespread substrates in nanotechnology. By studying
the propagation of PhPs on α-MoO3 flakes with different thicknesses laying on
SiO2 substrates via near-field microscopy (s-SNOM), we extract locally the infrared
permittivity of SiO2. Our work reveals PhPs nanoimaging as a versatile method
for the quantitative characterization of the local optical properties of dielectric
substrates, crucial for understanding and predicting the response of nanomaterials
and for the future scalability of integrated nanophotonic devices. '
acknowledgement: "P.A.-M. acknowledges financial support through JAE Intro program
from the Superior\r\nCouncil of Scientific Investigations and the Spanish Ministry
of Science and Innovation (grant number JAEINT_20_00589). G.Á.-P. and J.T.-G. acknowledge
financial support through the Severo Ochoa Program from the Government of the Principality
of Asturias (grant numbers PA-20-PF-BP19-053 and PA-18-PF-BP17-126, respectively).
J.M.-S. acknowledges financial support from the Ramón y Cajal Program of the Government
of Spain (RYC2018-026196-I) and the Spanish Ministry of Science and Innovation (State
Plan for Scientific and Technical Research and Innovation grant number PID2019-110308GA-I00).
P.A.-G. acknowledges support from the European Research Council under starting grant
no. 715496, 2DNANOPTICA and the Spanish Ministry of Science and Innovation (State
Plan for Scientific and Technical Research and Innovation grant number PID2019-111156GB-I00)."
article_number: '120'
article_processing_charge: No
article_type: original
author:
- first_name: Patricia
full_name: Aguilar-Merino, Patricia
last_name: Aguilar-Merino
- first_name: Gonzalo
full_name: Álvarez-Pérez, Gonzalo
last_name: Álvarez-Pérez
- first_name: Javier
full_name: Taboada-Gutiérrez, Javier
last_name: Taboada-Gutiérrez
- first_name: Jiahua
full_name: Duan, Jiahua
last_name: Duan
- first_name: Ivan
full_name: Prieto Gonzalez, Ivan
id: 2A307FE2-F248-11E8-B48F-1D18A9856A87
last_name: Prieto Gonzalez
orcid: 0000-0002-7370-5357
- first_name: Luis Manuel
full_name: Álvarez-Prado, Luis Manuel
last_name: Álvarez-Prado
- first_name: Alexey Y.
full_name: Nikitin, Alexey Y.
last_name: Nikitin
- first_name: Javier
full_name: Martín-Sánchez, Javier
last_name: Martín-Sánchez
- first_name: Pablo
full_name: Alonso-González, Pablo
last_name: Alonso-González
citation:
ama: Aguilar-Merino P, Álvarez-Pérez G, Taboada-Gutiérrez J, et al. Extracting the
infrared permittivity of SiO2 substrates locally by near-field imaging of phonon
polaritons in a van der Waals crystal. Nanomaterials. 2021;11(1). doi:10.3390/nano11010120
apa: Aguilar-Merino, P., Álvarez-Pérez, G., Taboada-Gutiérrez, J., Duan, J., Prieto
Gonzalez, I., Álvarez-Prado, L. M., … Alonso-González, P. (2021). Extracting the
infrared permittivity of SiO2 substrates locally by near-field imaging of phonon
polaritons in a van der Waals crystal. Nanomaterials. MDPI. https://doi.org/10.3390/nano11010120
chicago: Aguilar-Merino, Patricia, Gonzalo Álvarez-Pérez, Javier Taboada-Gutiérrez,
Jiahua Duan, Ivan Prieto Gonzalez, Luis Manuel Álvarez-Prado, Alexey Y. Nikitin,
Javier Martín-Sánchez, and Pablo Alonso-González. “Extracting the Infrared Permittivity
of SiO2 Substrates Locally by Near-Field Imaging of Phonon Polaritons in a van
Der Waals Crystal.” Nanomaterials. MDPI, 2021. https://doi.org/10.3390/nano11010120.
ieee: P. Aguilar-Merino et al., “Extracting the infrared permittivity of
SiO2 substrates locally by near-field imaging of phonon polaritons in a van der
Waals crystal,” Nanomaterials, vol. 11, no. 1. MDPI, 2021.
ista: Aguilar-Merino P, Álvarez-Pérez G, Taboada-Gutiérrez J, Duan J, Prieto Gonzalez
I, Álvarez-Prado LM, Nikitin AY, Martín-Sánchez J, Alonso-González P. 2021. Extracting
the infrared permittivity of SiO2 substrates locally by near-field imaging of
phonon polaritons in a van der Waals crystal. Nanomaterials. 11(1), 120.
mla: Aguilar-Merino, Patricia, et al. “Extracting the Infrared Permittivity of SiO2
Substrates Locally by Near-Field Imaging of Phonon Polaritons in a van Der Waals
Crystal.” Nanomaterials, vol. 11, no. 1, 120, MDPI, 2021, doi:10.3390/nano11010120.
short: P. Aguilar-Merino, G. Álvarez-Pérez, J. Taboada-Gutiérrez, J. Duan, I. Prieto
Gonzalez, L.M. Álvarez-Prado, A.Y. Nikitin, J. Martín-Sánchez, P. Alonso-González,
Nanomaterials 11 (2021).
date_created: 2021-01-24T23:01:09Z
date_published: 2021-01-07T00:00:00Z
date_updated: 2023-08-07T13:35:50Z
day: '07'
ddc:
- '620'
department:
- _id: NanoFab
doi: 10.3390/nano11010120
external_id:
isi:
- '000610636600001'
pmid:
- '33430225'
file:
- access_level: open_access
checksum: 1edc13eeda83df5cd9fff9504727b1f5
content_type: application/pdf
creator: dernst
date_created: 2021-01-25T08:02:32Z
date_updated: 2021-01-25T08:02:32Z
file_id: '9042'
file_name: 2020_Nanomaterials_Aguilar_Merino.pdf
file_size: 2730267
relation: main_file
success: 1
file_date_updated: 2021-01-25T08:02:32Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nanomaterials
publication_identifier:
eissn:
- '20794991'
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Extracting the infrared permittivity of SiO2 substrates locally by near-field
imaging of phonon polaritons in a van der Waals crystal
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2021'
...
---
_id: '9262'
abstract:
- lang: eng
text: Sequence-specific oligomers with predictable folding patterns, i.e., foldamers,
provide new opportunities to mimic α-helical peptides and design inhibitors of
protein-protein interactions. One major hurdle of this strategy is to retain the
correct orientation of key side chains involved in protein surface recognition.
Here, we show that the structural plasticity of a foldamer backbone may notably
contribute to the required spatial adjustment for optimal interaction with the
protein surface. By using oligoureas as α helix mimics, we designed a foldamer/peptide
hybrid inhibitor of histone chaperone ASF1, a key regulator of chromatin dynamics.
The crystal structure of its complex with ASF1 reveals a notable plasticity of
the urea backbone, which adapts to the ASF1 surface to maintain the same binding
interface. One additional benefit of generating ASF1 ligands with nonpeptide oligourea
segments is the resistance to proteolysis in human plasma, which was highly improved
compared to the cognate α-helical peptide.
acknowledgement: 'We thank the Synchrotron SOLEIL, the European Synchrotron Radiation
Facility (ESRF), and the French Infrastructure for Integrated Structural Biology
(FRISBI) ANR-10-INBS-05. We are particularly grateful to A. Clavier and A. Campalans
for help in setting up and performing the cell penetration assays. Funding: Research
was funded by the French Centre National de Recherche Scientifique (CNRS), the Commissariat
à l’Energie Atomique (CEA), University of Bordeaux, University Paris-Saclay, and
the Synchrotron Soleil. The project was supported by the ANR 2007 BREAKABOUND (JC-07-216078),
2011 BIPBIP (ANR-10-BINF-0003), 2012 CHAPINHIB (ANR-12-BSV5-0022-01), 2015 CHIPSET
(ANR-15-CE11-008-01), 2015 HIMPP2I (ANR-15-CE07-0010), and the program labeled by
the ARC foundation 2016 PGA1*20160203953). M.B. was supported by Canceropole (Paris,
France) and a grant for young researchers from La Ligue contre le Cancer. J.M. was
supported by La Ligue contre le Cancer.'
article_number: eabd9153
article_processing_charge: No
article_type: original
author:
- first_name: Johanne
full_name: Mbianda, Johanne
last_name: Mbianda
- first_name: May M
full_name: Bakail, May M
id: FB3C3F8E-522F-11EA-B186-22963DDC885E
last_name: Bakail
orcid: 0000-0002-9592-1587
- first_name: Christophe
full_name: André, Christophe
last_name: André
- first_name: Gwenaëlle
full_name: Moal, Gwenaëlle
last_name: Moal
- first_name: Marie E.
full_name: Perrin, Marie E.
last_name: Perrin
- first_name: Guillaume
full_name: Pinna, Guillaume
last_name: Pinna
- first_name: Raphaël
full_name: Guerois, Raphaël
last_name: Guerois
- first_name: Francois
full_name: Becher, Francois
last_name: Becher
- first_name: Pierre
full_name: Legrand, Pierre
last_name: Legrand
- first_name: Seydou
full_name: Traoré, Seydou
last_name: Traoré
- first_name: Céline
full_name: Douat, Céline
last_name: Douat
- first_name: Gilles
full_name: Guichard, Gilles
last_name: Guichard
- first_name: Françoise
full_name: Ochsenbein, Françoise
last_name: Ochsenbein
citation:
ama: Mbianda J, Bakail MM, André C, et al. Optimal anchoring of a foldamer inhibitor
of ASF1 histone chaperone through backbone plasticity. Science Advances.
2021;7(12). doi:10.1126/sciadv.abd9153
apa: Mbianda, J., Bakail, M. M., André, C., Moal, G., Perrin, M. E., Pinna, G.,
… Ochsenbein, F. (2021). Optimal anchoring of a foldamer inhibitor of ASF1 histone
chaperone through backbone plasticity. Science Advances. American Association
for the Advancement of Science. https://doi.org/10.1126/sciadv.abd9153
chicago: Mbianda, Johanne, May M Bakail, Christophe André, Gwenaëlle Moal, Marie
E. Perrin, Guillaume Pinna, Raphaël Guerois, et al. “Optimal Anchoring of a Foldamer
Inhibitor of ASF1 Histone Chaperone through Backbone Plasticity.” Science Advances.
American Association for the Advancement of Science, 2021. https://doi.org/10.1126/sciadv.abd9153.
ieee: J. Mbianda et al., “Optimal anchoring of a foldamer inhibitor of ASF1
histone chaperone through backbone plasticity,” Science Advances, vol.
7, no. 12. American Association for the Advancement of Science, 2021.
ista: Mbianda J, Bakail MM, André C, Moal G, Perrin ME, Pinna G, Guerois R, Becher
F, Legrand P, Traoré S, Douat C, Guichard G, Ochsenbein F. 2021. Optimal anchoring
of a foldamer inhibitor of ASF1 histone chaperone through backbone plasticity.
Science Advances. 7(12), eabd9153.
mla: Mbianda, Johanne, et al. “Optimal Anchoring of a Foldamer Inhibitor of ASF1
Histone Chaperone through Backbone Plasticity.” Science Advances, vol.
7, no. 12, eabd9153, American Association for the Advancement of Science, 2021,
doi:10.1126/sciadv.abd9153.
short: J. Mbianda, M.M. Bakail, C. André, G. Moal, M.E. Perrin, G. Pinna, R. Guerois,
F. Becher, P. Legrand, S. Traoré, C. Douat, G. Guichard, F. Ochsenbein, Science
Advances 7 (2021).
date_created: 2021-03-22T07:14:03Z
date_published: 2021-03-19T00:00:00Z
date_updated: 2023-08-07T14:20:26Z
day: '19'
ddc:
- '570'
department:
- _id: CampIT
doi: 10.1126/sciadv.abd9153
external_id:
isi:
- '000633443000011'
pmid:
- '33741589'
file:
- access_level: open_access
checksum: 737624cd0e630ffa7c52797a690500e3
content_type: application/pdf
creator: dernst
date_created: 2021-03-22T12:49:00Z
date_updated: 2021-03-22T12:49:00Z
file_id: '9280'
file_name: 2021_ScienceAdv_Mbianda.pdf
file_size: 837156
relation: main_file
success: 1
file_date_updated: 2021-03-22T12:49:00Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
issue: '12'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
issn:
- 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
status: public
title: Optimal anchoring of a foldamer inhibitor of ASF1 histone chaperone through
backbone plasticity
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2021'
...
---
_id: '9259'
abstract:
- lang: eng
text: Gradients of chemokines and growth factors guide migrating cells and morphogenetic
processes. Migration of antigen-presenting dendritic cells from the interstitium
into the lymphatic system is dependent on chemokine CCL21, which is secreted by
endothelial cells of the lymphatic capillary, binds heparan sulfates and forms
gradients decaying into the interstitium. Despite the importance of CCL21 gradients,
and chemokine gradients in general, the mechanisms of gradient formation are unclear.
Studies on fibroblast growth factors have shown that limited diffusion is crucial
for gradient formation. Here, we used the mouse dermis as a model tissue to address
the necessity of CCL21 anchoring to lymphatic capillary heparan sulfates in the
formation of interstitial CCL21 gradients. Surprisingly, the absence of lymphatic
endothelial heparan sulfates resulted only in a modest decrease of CCL21 levels
at the lymphatic capillaries and did neither affect interstitial CCL21 gradient
shape nor dendritic cell migration toward lymphatic capillaries. Thus, heparan
sulfates at the level of the lymphatic endothelium are dispensable for the formation
of a functional CCL21 gradient.
acknowledgement: "This work was supported by Sigrid Juselius fellowship (KV), University
of Helsinki 3-year research grant (KV), Academy of Finland Research fellow funding
(315710, to KV), the European Research Council (ERC CoG 724373 to MS), and by the
Austrian Science foundation (FWF) (Y564-B12 START award to MS).\r\nTaija Mäkinen
is acknowledged for providing Prox1CreERT2 transgenic mice and Yu Yamaguchi for
providing the conditional Ext1 mouse strain."
article_number: '630002'
article_processing_charge: No
article_type: original
author:
- first_name: Kari
full_name: Vaahtomeri, Kari
id: 368EE576-F248-11E8-B48F-1D18A9856A87
last_name: Vaahtomeri
orcid: 0000-0001-7829-3518
- first_name: Christine
full_name: Moussion, Christine
id: 3356F664-F248-11E8-B48F-1D18A9856A87
last_name: Moussion
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. Shape and function of interstitial
chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic
endothelium. Frontiers in Immunology. 2021;12. doi:10.3389/fimmu.2021.630002
apa: Vaahtomeri, K., Moussion, C., Hauschild, R., & Sixt, M. K. (2021). Shape
and function of interstitial chemokine CCL21 gradients are independent of heparan
sulfates produced by lymphatic endothelium. Frontiers in Immunology. Frontiers.
https://doi.org/10.3389/fimmu.2021.630002
chicago: Vaahtomeri, Kari, Christine Moussion, Robert Hauschild, and Michael K Sixt.
“Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent
of Heparan Sulfates Produced by Lymphatic Endothelium.” Frontiers in Immunology.
Frontiers, 2021. https://doi.org/10.3389/fimmu.2021.630002.
ieee: K. Vaahtomeri, C. Moussion, R. Hauschild, and M. K. Sixt, “Shape and function
of interstitial chemokine CCL21 gradients are independent of heparan sulfates
produced by lymphatic endothelium,” Frontiers in Immunology, vol. 12. Frontiers,
2021.
ista: Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. 2021. Shape and function of
interstitial chemokine CCL21 gradients are independent of heparan sulfates produced
by lymphatic endothelium. Frontiers in Immunology. 12, 630002.
mla: Vaahtomeri, Kari, et al. “Shape and Function of Interstitial Chemokine CCL21
Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.”
Frontiers in Immunology, vol. 12, 630002, Frontiers, 2021, doi:10.3389/fimmu.2021.630002.
short: K. Vaahtomeri, C. Moussion, R. Hauschild, M.K. Sixt, Frontiers in Immunology
12 (2021).
date_created: 2021-03-21T23:01:20Z
date_published: 2021-02-25T00:00:00Z
date_updated: 2023-08-07T14:18:26Z
day: '25'
ddc:
- '570'
department:
- _id: MiSi
- _id: Bio
doi: 10.3389/fimmu.2021.630002
ec_funded: 1
external_id:
isi:
- '000627134400001'
pmid:
- '33717158'
file:
- access_level: open_access
checksum: 663f5a48375e42afa4bfef58d42ec186
content_type: application/pdf
creator: dernst
date_created: 2021-03-22T12:08:26Z
date_updated: 2021-03-22T12:08:26Z
file_id: '9277'
file_name: 2021_FrontiersImmumo_Vaahtomeri.pdf
file_size: 3740146
relation: main_file
success: 1
file_date_updated: 2021-03-22T12:08:26Z
has_accepted_license: '1'
intvolume: ' 12'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '724373'
name: Cellular navigation along spatial gradients
- _id: 25A8E5EA-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Y 564-B12
name: Cytoskeletal force generation and force transduction of migrating leukocytes
publication: Frontiers in Immunology
publication_identifier:
eissn:
- 1664-3224
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: Shape and function of interstitial chemokine CCL21 gradients are independent
of heparan sulfates produced by lymphatic endothelium
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2021'
...
---
_id: '9329'
abstract:
- lang: eng
text: "Background: To understand information coding in single neurons, it is necessary
to analyze subthreshold synaptic events, action potentials (APs), and their interrelation
in different behavioral states. However, detecting excitatory postsynaptic potentials
(EPSPs) or currents (EPSCs) in behaving animals remains challenging, because of
unfavorable signal-to-noise ratio, high frequency, fluctuating amplitude, and
variable time course of synaptic events.\r\nNew method: We developed a method
for synaptic event detection, termed MOD (Machine-learning Optimal-filtering Detection-procedure),
which combines concepts of supervised machine learning and optimal Wiener filtering.
Experts were asked to manually score short epochs of data. The algorithm was trained
to obtain the optimal filter coefficients of a Wiener filter and the optimal detection
threshold. Scored and unscored data were then processed with the optimal filter,
and events were detected as peaks above threshold.\r\nResults: We challenged MOD
with EPSP traces in vivo in mice during spatial navigation and EPSC traces in
vitro in slices under conditions of enhanced transmitter release. The area under
the curve (AUC) of the receiver operating characteristics (ROC) curve was, on
average, 0.894 for in vivo and 0.969 for in vitro data sets, indicating high detection
accuracy and efficiency.\r\nComparison with existing methods: When benchmarked
using a (1 − AUC)−1 metric, MOD outperformed previous methods (template-fit, deconvolution,
and Bayesian methods) by an average factor of 3.13 for in vivo data sets, but
showed comparable (template-fit, deconvolution) or higher (Bayesian) computational
efficacy.\r\nConclusions: MOD may become an important new tool for large-scale,
real-time analysis of synaptic activity."
acknowledged_ssus:
- _id: SSU
acknowledgement: This project has received funding from the European Research Council
(ERC) under the European Union’s Horizon 2020 research and innovation programme
(grant agreement number 692692 to P.J.) and the Fond zur Förderung der Wissenschaftlichen
Forschung (Z 312-B27, Wittgenstein award to P.J.). We thank Drs. Jozsef Csicsvari,
Christoph Lampert, and Federico Stella for critically reading previous manuscript
versions. We are also grateful to Drs. Josh Merel and Ben Shababo for their help
with applying the Bayesian detection method to our data. We also thank Florian Marr
for technical assistance, Eleftheria Kralli-Beller for manuscript editing, and the
Scientific Service Units of IST Austria for efficient support.
article_number: '109125'
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Xiaomin
full_name: Zhang, Xiaomin
id: 423EC9C2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
- first_name: Alois
full_name: Schlögl, Alois
id: 45BF87EE-F248-11E8-B48F-1D18A9856A87
last_name: Schlögl
orcid: 0000-0002-5621-8100
- first_name: David H
full_name: Vandael, David H
id: 3AE48E0A-F248-11E8-B48F-1D18A9856A87
last_name: Vandael
orcid: 0000-0001-7577-1676
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Zhang X, Schlögl A, Vandael DH, Jonas PM. MOD: A novel machine-learning optimal-filtering
method for accurate and efficient detection of subthreshold synaptic events in
vivo. Journal of Neuroscience Methods. 2021;357(6). doi:10.1016/j.jneumeth.2021.109125'
apa: 'Zhang, X., Schlögl, A., Vandael, D. H., & Jonas, P. M. (2021). MOD: A
novel machine-learning optimal-filtering method for accurate and efficient detection
of subthreshold synaptic events in vivo. Journal of Neuroscience Methods.
Elsevier. https://doi.org/10.1016/j.jneumeth.2021.109125'
chicago: 'Zhang, Xiaomin, Alois Schlögl, David H Vandael, and Peter M Jonas. “MOD:
A Novel Machine-Learning Optimal-Filtering Method for Accurate and Efficient Detection
of Subthreshold Synaptic Events in Vivo.” Journal of Neuroscience Methods.
Elsevier, 2021. https://doi.org/10.1016/j.jneumeth.2021.109125.'
ieee: 'X. Zhang, A. Schlögl, D. H. Vandael, and P. M. Jonas, “MOD: A novel machine-learning
optimal-filtering method for accurate and efficient detection of subthreshold
synaptic events in vivo,” Journal of Neuroscience Methods, vol. 357, no.
6. Elsevier, 2021.'
ista: 'Zhang X, Schlögl A, Vandael DH, Jonas PM. 2021. MOD: A novel machine-learning
optimal-filtering method for accurate and efficient detection of subthreshold
synaptic events in vivo. Journal of Neuroscience Methods. 357(6), 109125.'
mla: 'Zhang, Xiaomin, et al. “MOD: A Novel Machine-Learning Optimal-Filtering Method
for Accurate and Efficient Detection of Subthreshold Synaptic Events in Vivo.”
Journal of Neuroscience Methods, vol. 357, no. 6, 109125, Elsevier, 2021,
doi:10.1016/j.jneumeth.2021.109125.'
short: X. Zhang, A. Schlögl, D.H. Vandael, P.M. Jonas, Journal of Neuroscience Methods
357 (2021).
date_created: 2021-04-18T22:01:39Z
date_published: 2021-03-09T00:00:00Z
date_updated: 2023-08-07T14:36:14Z
day: '09'
ddc:
- '570'
department:
- _id: PeJo
- _id: ScienComp
doi: 10.1016/j.jneumeth.2021.109125
ec_funded: 1
external_id:
isi:
- '000661088500005'
file:
- access_level: open_access
checksum: 2a5800d91b96d08b525e17319dcd5e44
content_type: application/pdf
creator: dernst
date_created: 2021-04-19T08:30:22Z
date_updated: 2021-04-19T08:30:22Z
file_id: '9339'
file_name: 2021_JourNeuroscienceMeth_Zhang.pdf
file_size: 6924738
relation: main_file
success: 1
file_date_updated: 2021-04-19T08:30:22Z
has_accepted_license: '1'
intvolume: ' 357'
isi: 1
issue: '6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 25B7EB9E-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '692692'
name: Biophysics and circuit function of a giant cortical glumatergic synapse
- _id: 25C5A090-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z00312
name: The Wittgenstein Prize
publication: Journal of Neuroscience Methods
publication_identifier:
eissn:
- 1872-678X
issn:
- 0165-0270
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'MOD: A novel machine-learning optimal-filtering method for accurate and efficient
detection of subthreshold synaptic events in vivo'
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 357
year: '2021'
...
---
_id: '9330'
abstract:
- lang: eng
text: In nerve cells the genes encoding for α2δ subunits of voltage-gated calcium
channels have been linked to synaptic functions and neurological disease. Here
we show that α2δ subunits are essential for the formation and organization of
glutamatergic synapses. Using a cellular α2δ subunit triple-knockout/knockdown
model, we demonstrate a failure in presynaptic differentiation evidenced by defective
presynaptic calcium channel clustering and calcium influx, smaller presynaptic
active zones, and a strongly reduced accumulation of presynaptic vesicle-associated
proteins (synapsin and vGLUT). The presynaptic defect is associated with the downscaling
of postsynaptic AMPA receptors and the postsynaptic density. The role of α2δ isoforms
as synaptic organizers is highly redundant, as each individual α2δ isoform can
rescue presynaptic calcium channel trafficking and expression of synaptic proteins.
Moreover, α2δ-2 and α2δ-3 with mutated metal ion-dependent adhesion sites can
fully rescue presynaptic synapsin expression but only partially calcium channel
trafficking, suggesting that the regulatory role of α2δ subunits is independent
from its role as a calcium channel subunit. Our findings influence the current
view on excitatory synapse formation. First, our study suggests that postsynaptic
differentiation is secondary to presynaptic differentiation. Second, the dependence
of presynaptic differentiation on α2δ implicates α2δ subunits as potential nucleation
points for the organization of synapses. Finally, our results suggest that α2δ
subunits act as transsynaptic organizers of glutamatergic synapses, thereby aligning
the synaptic active zone with the postsynaptic density.
acknowledged_ssus:
- _id: EM-Fac
acknowledgement: "We thank Arnold Schwartz for providing α2δ-1 knockout mice; Ariane
Benedetti, Sabine Baumgartner, Sandra Demetz, and Irene Mahlknecht for technical
support; Nadine Ortner and Andreas Lieb for electrophysiological experiments; the
team of the Electron Microscopy Facility at the Institute of Science and Technology
Austria for technical support related to ultrastructural analysis; Hermann Dietrich
and Anja Beierfuß and her team for animal care; Jutta Engel and Jörg Striessnig
for critical discussions; and Bruno Benedetti and Bernhard Flucher for critical
discussions and reading the manuscript. This study was supported by Austrian Science
Fund Grants P24079, F44060, F44150, and DOC30-B30 (to G.J.O.) and T855 (to M.C.),
European Research Council Grant AdG 694539 (to R.S.), Deutsche Forschungsgemeinschaft\r\nGrant
SFB1348-TP A03 (to M.M.), and Interdisziplinäre Zentrum für Klinische Forschung
Münster Grant Mi3/004/19 (to M.M.). This work is part of the PhD theses of C.L.S.,
S.M.G., and C.A."
article_processing_charge: No
article_type: original
author:
- first_name: Clemens L.
full_name: Schöpf, Clemens L.
last_name: Schöpf
- first_name: Cornelia
full_name: Ablinger, Cornelia
last_name: Ablinger
- first_name: Stefanie M.
full_name: Geisler, Stefanie M.
last_name: Geisler
- first_name: Ruslan I.
full_name: Stanika, Ruslan I.
last_name: Stanika
- first_name: Marta
full_name: Campiglio, Marta
last_name: Campiglio
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Benedikt
full_name: Nimmervoll, Benedikt
last_name: Nimmervoll
- first_name: Bettina
full_name: Schlick, Bettina
last_name: Schlick
- first_name: Johannes
full_name: Brockhaus, Johannes
last_name: Brockhaus
- first_name: Markus
full_name: Missler, Markus
last_name: Missler
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Gerald J.
full_name: Obermair, Gerald J.
last_name: Obermair
citation:
ama: Schöpf CL, Ablinger C, Geisler SM, et al. Presynaptic α2δ subunits are key
organizers of glutamatergic synapses. PNAS. 2021;118(14). doi:10.1073/pnas.1920827118
apa: Schöpf, C. L., Ablinger, C., Geisler, S. M., Stanika, R. I., Campiglio, M.,
Kaufmann, W., … Obermair, G. J. (2021). Presynaptic α2δ subunits are key organizers
of glutamatergic synapses. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1920827118
chicago: Schöpf, Clemens L., Cornelia Ablinger, Stefanie M. Geisler, Ruslan I. Stanika,
Marta Campiglio, Walter Kaufmann, Benedikt Nimmervoll, et al. “Presynaptic Α2δ
Subunits Are Key Organizers of Glutamatergic Synapses.” PNAS. National
Academy of Sciences, 2021. https://doi.org/10.1073/pnas.1920827118.
ieee: C. L. Schöpf et al., “Presynaptic α2δ subunits are key organizers of
glutamatergic synapses,” PNAS, vol. 118, no. 14. National Academy of Sciences,
2021.
ista: Schöpf CL, Ablinger C, Geisler SM, Stanika RI, Campiglio M, Kaufmann W, Nimmervoll
B, Schlick B, Brockhaus J, Missler M, Shigemoto R, Obermair GJ. 2021. Presynaptic
α2δ subunits are key organizers of glutamatergic synapses. PNAS. 118(14).
mla: Schöpf, Clemens L., et al. “Presynaptic Α2δ Subunits Are Key Organizers of
Glutamatergic Synapses.” PNAS, vol. 118, no. 14, National Academy of Sciences,
2021, doi:10.1073/pnas.1920827118.
short: C.L. Schöpf, C. Ablinger, S.M. Geisler, R.I. Stanika, M. Campiglio, W. Kaufmann,
B. Nimmervoll, B. Schlick, J. Brockhaus, M. Missler, R. Shigemoto, G.J. Obermair,
PNAS 118 (2021).
date_created: 2021-04-18T22:01:40Z
date_published: 2021-04-06T00:00:00Z
date_updated: 2023-08-08T13:08:47Z
day: '06'
ddc:
- '570'
department:
- _id: EM-Fac
- _id: RySh
doi: 10.1073/pnas.1920827118
ec_funded: 1
external_id:
isi:
- '000637398300002'
file:
- access_level: open_access
checksum: dd014f68ae9d7d8d8fc4139a24e04506
content_type: application/pdf
creator: dernst
date_created: 2021-04-19T10:10:56Z
date_updated: 2021-04-19T10:10:56Z
file_id: '9340'
file_name: 2021_PNAS_Schoepf.pdf
file_size: 2603911
relation: main_file
success: 1
file_date_updated: 2021-04-19T10:10:56Z
has_accepted_license: '1'
intvolume: ' 118'
isi: 1
issue: '14'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '694539'
name: 'In situ analysis of single channel subunit composition in neurons: physiological
implication in synaptic plasticity and behaviour'
publication: PNAS
publication_identifier:
eissn:
- 1091-6490
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Presynaptic α2δ subunits are key organizers of glutamatergic synapses
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 118
year: '2021'
...
---
_id: '9334'
abstract:
- lang: eng
text: 'Polaritons with directional in-plane propagation and ultralow losses in van
der Waals (vdW) crystals promise unprecedented manipulation of light at the nanoscale.
However, these polaritons present a crucial limitation: their directional propagation
is intrinsically determined by the crystal structure of the host material, imposing
forbidden directions of propagation. Here, we demonstrate that directional polaritons
(in-plane hyperbolic phonon polaritons) in a vdW crystal (α-phase molybdenum trioxide)
can be directed along forbidden directions by inducing an optical topological
transition, which emerges when the slab is placed on a substrate with a given
negative permittivity (4H–silicon carbide). By visualizing the transition in real
space, we observe exotic polaritonic states between mutually orthogonal hyperbolic
regimes, which unveil the topological origin of the transition: a gap opening
in the dispersion. This work provides insights into optical topological transitions
in vdW crystals, which introduce a route to direct light at the nanoscale.'
acknowledgement: 'G.Á.-P. and J.T.-G. acknowledge support through the Severo Ochoa
Program from the government of the Principality of Asturias (grant nos. PA20-PF-BP19-053
and PA-18-PF-BP17-126, respectively). K.V.V. and V.S.V. acknowledge the Ministry
of Science and Higher Education of the Russian Federation (no. 0714-2020-0002).
J. M.-S. acknowledges financial support through the Ramón y Cajal Program from the
government of Spain and FSE (RYC2018-026196-I). A.Y.N. acknowledges the Spanish
Ministry of Science, Innovation and Universities (national project no. MAT201788358-C3-3-R),
and the Basque Department of Education (PIBA-2020-1-0014). P.A.-G. acknowledges
support from the European Research Council under starting grant no. 715496, 2DNANOPTICA. '
article_number: eabf2690
article_processing_charge: No
article_type: original
author:
- first_name: J.
full_name: Duan, J.
last_name: Duan
- first_name: G.
full_name: Álvarez-Pérez, G.
last_name: Álvarez-Pérez
- first_name: K. V.
full_name: Voronin, K. V.
last_name: Voronin
- first_name: Ivan
full_name: Prieto Gonzalez, Ivan
id: 2A307FE2-F248-11E8-B48F-1D18A9856A87
last_name: Prieto Gonzalez
orcid: 0000-0002-7370-5357
- first_name: J.
full_name: Taboada-Gutiérrez, J.
last_name: Taboada-Gutiérrez
- first_name: V. S.
full_name: Volkov, V. S.
last_name: Volkov
- first_name: J.
full_name: Martín-Sánchez, J.
last_name: Martín-Sánchez
- first_name: A. Y.
full_name: Nikitin, A. Y.
last_name: Nikitin
- first_name: P.
full_name: Alonso-González, P.
last_name: Alonso-González
citation:
ama: Duan J, Álvarez-Pérez G, Voronin KV, et al. Enabling propagation of anisotropic
polaritons along forbidden directions via a topological transition. Science
Advances. 2021;7(14). doi:10.1126/sciadv.abf2690
apa: Duan, J., Álvarez-Pérez, G., Voronin, K. V., Prieto Gonzalez, I., Taboada-Gutiérrez,
J., Volkov, V. S., … Alonso-González, P. (2021). Enabling propagation of anisotropic
polaritons along forbidden directions via a topological transition. Science
Advances. AAAS. https://doi.org/10.1126/sciadv.abf2690
chicago: Duan, J., G. Álvarez-Pérez, K. V. Voronin, Ivan Prieto Gonzalez, J. Taboada-Gutiérrez,
V. S. Volkov, J. Martín-Sánchez, A. Y. Nikitin, and P. Alonso-González. “Enabling
Propagation of Anisotropic Polaritons along Forbidden Directions via a Topological
Transition.” Science Advances. AAAS, 2021. https://doi.org/10.1126/sciadv.abf2690.
ieee: J. Duan et al., “Enabling propagation of anisotropic polaritons along
forbidden directions via a topological transition,” Science Advances, vol.
7, no. 14. AAAS, 2021.
ista: Duan J, Álvarez-Pérez G, Voronin KV, Prieto Gonzalez I, Taboada-Gutiérrez
J, Volkov VS, Martín-Sánchez J, Nikitin AY, Alonso-González P. 2021. Enabling
propagation of anisotropic polaritons along forbidden directions via a topological
transition. Science Advances. 7(14), eabf2690.
mla: Duan, J., et al. “Enabling Propagation of Anisotropic Polaritons along Forbidden
Directions via a Topological Transition.” Science Advances, vol. 7, no.
14, eabf2690, AAAS, 2021, doi:10.1126/sciadv.abf2690.
short: J. Duan, G. Álvarez-Pérez, K.V. Voronin, I. Prieto Gonzalez, J. Taboada-Gutiérrez,
V.S. Volkov, J. Martín-Sánchez, A.Y. Nikitin, P. Alonso-González, Science Advances
7 (2021).
date_created: 2021-04-18T22:01:42Z
date_published: 2021-04-02T00:00:00Z
date_updated: 2023-08-08T13:11:31Z
day: '02'
ddc:
- '530'
department:
- _id: NanoFab
doi: 10.1126/sciadv.abf2690
external_id:
isi:
- '000636455600027'
pmid:
- '33811076'
file:
- access_level: open_access
checksum: 4b383d4a1d484a71bbc64ecf401bbdbb
content_type: application/pdf
creator: dernst
date_created: 2021-04-19T11:17:29Z
date_updated: 2021-04-19T11:17:29Z
file_id: '9343'
file_name: 2021_ScienceAdv_Duan.pdf
file_size: 717489
relation: main_file
success: 1
file_date_updated: 2021-04-19T11:17:29Z
has_accepted_license: '1'
intvolume: ' 7'
isi: 1
issue: '14'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Science Advances
publication_identifier:
eissn:
- '23752548'
publication_status: published
publisher: AAAS
quality_controlled: '1'
scopus_import: '1'
status: public
title: Enabling propagation of anisotropic polaritons along forbidden directions via
a topological transition
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 7
year: '2021'
...
---
_id: '9363'
abstract:
- lang: eng
text: Optogenetics has been harnessed to shed new mechanistic light on current and
future therapeutic strategies. This has been to date achieved by the regulation
of ion flow and electrical signals in neuronal cells and neural circuits that
are known to be affected by disease. In contrast, the optogenetic delivery of
trophic biochemical signals, which support cell survival and are implicated in
degenerative disorders, has never been demonstrated in an animal model of disease.
Here, we reengineered the human and Drosophila melanogaster REarranged during
Transfection (hRET and dRET) receptors to be activated by light, creating one-component
optogenetic tools termed Opto-hRET and Opto-dRET. Upon blue light stimulation,
these receptors robustly induced the MAPK/ERK proliferative signaling pathway
in cultured cells. In PINK1B9 flies that exhibit loss of PTEN-induced putative
kinase 1 (PINK1), a kinase associated with familial Parkinson’s disease (PD),
light activation of Opto-dRET suppressed mitochondrial defects, tissue degeneration
and behavioral deficits. In human cells with PINK1 loss-of-function, mitochondrial
fragmentation was rescued using Opto-dRET via the PI3K/NF-кB pathway. Our results
demonstrate that a light-activated receptor can ameliorate disease hallmarks in
a genetic model of PD. The optogenetic delivery of trophic signals is cell type-specific
and reversible and thus has the potential to inspire novel strategies towards
a spatio-temporal regulation of tissue repair.
acknowledgement: We thank R. Cagan, A. Whitworth and J. Nagpal for fly lines and advice,
S. Herlitze for provision of a tissue culture illuminator, and Verian Bader for
help with statistical analysis.
article_processing_charge: No
author:
- first_name: Álvaro
full_name: Inglés Prieto, Álvaro
id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
last_name: Inglés Prieto
orcid: 0000-0002-5409-8571
- first_name: Nikolas
full_name: Furthmann, Nikolas
last_name: Furthmann
- first_name: Samuel H.
full_name: Crossman, Samuel H.
last_name: Crossman
- first_name: Alexandra Madelaine
full_name: Tichy, Alexandra Madelaine
last_name: Tichy
- first_name: Nina
full_name: Hoyer, Nina
last_name: Hoyer
- first_name: Meike
full_name: Petersen, Meike
last_name: Petersen
- first_name: Vanessa
full_name: Zheden, Vanessa
id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
last_name: Zheden
- first_name: Julia
full_name: Bicher, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Bicher
- first_name: Eva
full_name: Gschaider-Reichhart, Eva
id: 3FEE232A-F248-11E8-B48F-1D18A9856A87
last_name: Gschaider-Reichhart
orcid: 0000-0002-7218-7738
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Peter
full_name: Soba, Peter
last_name: Soba
- first_name: Konstanze F.
full_name: Winklhofer, Konstanze F.
last_name: Winklhofer
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
citation:
ama: Inglés Prieto Á, Furthmann N, Crossman SH, et al. Optogenetic delivery of trophic
signals in a genetic model of Parkinson’s disease. PLoS genetics. 2021;17(4):e1009479.
doi:10.1371/journal.pgen.1009479
apa: Inglés Prieto, Á., Furthmann, N., Crossman, S. H., Tichy, A. M., Hoyer, N.,
Petersen, M., … Janovjak, H. L. (2021). Optogenetic delivery of trophic signals
in a genetic model of Parkinson’s disease. PLoS Genetics. Public Library
of Science. https://doi.org/10.1371/journal.pgen.1009479
chicago: Inglés Prieto, Álvaro, Nikolas Furthmann, Samuel H. Crossman, Alexandra
Madelaine Tichy, Nina Hoyer, Meike Petersen, Vanessa Zheden, et al. “Optogenetic
Delivery of Trophic Signals in a Genetic Model of Parkinson’s Disease.” PLoS
Genetics. Public Library of Science, 2021. https://doi.org/10.1371/journal.pgen.1009479.
ieee: Á. Inglés Prieto et al., “Optogenetic delivery of trophic signals in
a genetic model of Parkinson’s disease,” PLoS genetics, vol. 17, no. 4.
Public Library of Science, p. e1009479, 2021.
ista: Inglés Prieto Á, Furthmann N, Crossman SH, Tichy AM, Hoyer N, Petersen M,
Zheden V, Bicher J, Gschaider-Reichhart E, György A, Siekhaus DE, Soba P, Winklhofer
KF, Janovjak HL. 2021. Optogenetic delivery of trophic signals in a genetic model
of Parkinson’s disease. PLoS genetics. 17(4), e1009479.
mla: Inglés Prieto, Álvaro, et al. “Optogenetic Delivery of Trophic Signals in a
Genetic Model of Parkinson’s Disease.” PLoS Genetics, vol. 17, no. 4, Public
Library of Science, 2021, p. e1009479, doi:10.1371/journal.pgen.1009479.
short: Á. Inglés Prieto, N. Furthmann, S.H. Crossman, A.M. Tichy, N. Hoyer, M. Petersen,
V. Zheden, J. Bicher, E. Gschaider-Reichhart, A. György, D.E. Siekhaus, P. Soba,
K.F. Winklhofer, H.L. Janovjak, PLoS Genetics 17 (2021) e1009479.
date_created: 2021-05-02T22:01:29Z
date_published: 2021-04-01T00:00:00Z
date_updated: 2023-08-08T13:17:47Z
day: '01'
ddc:
- '570'
department:
- _id: EM-Fac
- _id: LoSw
- _id: DaSi
doi: 10.1371/journal.pgen.1009479
external_id:
isi:
- '000640606700001'
file:
- access_level: open_access
checksum: 82a74668f863e8dfb22fdd4f845c92ce
content_type: application/pdf
creator: kschuh
date_created: 2021-05-04T09:05:27Z
date_updated: 2021-05-04T09:05:27Z
file_id: '9369'
file_name: 2021_PLOS_Ingles-Prieto.pdf
file_size: 3072764
relation: main_file
success: 1
file_date_updated: 2021-05-04T09:05:27Z
has_accepted_license: '1'
intvolume: ' 17'
isi: 1
issue: '4'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: e1009479
publication: PLoS genetics
publication_identifier:
eissn:
- '15537404'
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 17
year: '2021'
...
---
_id: '9361'
abstract:
- lang: eng
text: The multimeric matrix (M) protein of clinically relevant paramyxoviruses orchestrates
assembly and budding activity of viral particles at the plasma membrane (PM).
We identified within the canine distemper virus (CDV) M protein two microdomains,
potentially assuming α-helix structures, which are essential for membrane budding
activity. Remarkably, while two rationally designed microdomain M mutants (E89R,
microdomain 1 and L239D, microdomain 2) preserved proper folding, dimerization,
interaction with the nucleocapsid protein, localization at and deformation of
the PM, the virus-like particle formation, as well as production of infectious
virions (as monitored using a membrane budding-complementation system), were,
in sharp contrast, strongly impaired. Of major importance, raster image correlation
spectroscopy (RICS) revealed that both microdomains contributed to finely tune
M protein mobility specifically at the PM. Collectively, our data highlighted
the cornerstone membrane budding-priming activity of two spatially discrete M
microdomains, potentially by coordinating the assembly of productive higher oligomers
at the PM.
acknowledgement: This work was supported by the Swiss National Science Foundation
(referencenumber 310030_173185 to P. P.).
article_number: e01024-20
article_processing_charge: No
author:
- first_name: Matthieu
full_name: Gast, Matthieu
last_name: Gast
- first_name: Nicole P.
full_name: Kadzioch, Nicole P.
last_name: Kadzioch
- first_name: Doreen
full_name: Milius, Doreen
id: 384050BC-F248-11E8-B48F-1D18A9856A87
last_name: Milius
- first_name: Francesco
full_name: Origgi, Francesco
last_name: Origgi
- first_name: Philippe
full_name: Plattet, Philippe
last_name: Plattet
citation:
ama: Gast M, Kadzioch NP, Milius D, Origgi F, Plattet P. Oligomerization and cell
egress controlled by two microdomains of canine distemper virus matrix protein.
mSphere. 2021;6(2). doi:10.1128/mSphere.01024-20
apa: Gast, M., Kadzioch, N. P., Milius, D., Origgi, F., & Plattet, P. (2021).
Oligomerization and cell egress controlled by two microdomains of canine distemper
virus matrix protein. MSphere. American Society for Microbiology. https://doi.org/10.1128/mSphere.01024-20
chicago: Gast, Matthieu, Nicole P. Kadzioch, Doreen Milius, Francesco Origgi, and
Philippe Plattet. “Oligomerization and Cell Egress Controlled by Two Microdomains
of Canine Distemper Virus Matrix Protein.” MSphere. American Society for
Microbiology, 2021. https://doi.org/10.1128/mSphere.01024-20.
ieee: M. Gast, N. P. Kadzioch, D. Milius, F. Origgi, and P. Plattet, “Oligomerization
and cell egress controlled by two microdomains of canine distemper virus matrix
protein,” mSphere, vol. 6, no. 2. American Society for Microbiology, 2021.
ista: Gast M, Kadzioch NP, Milius D, Origgi F, Plattet P. 2021. Oligomerization
and cell egress controlled by two microdomains of canine distemper virus matrix
protein. mSphere. 6(2), e01024-20.
mla: Gast, Matthieu, et al. “Oligomerization and Cell Egress Controlled by Two Microdomains
of Canine Distemper Virus Matrix Protein.” MSphere, vol. 6, no. 2, e01024-20,
American Society for Microbiology, 2021, doi:10.1128/mSphere.01024-20.
short: M. Gast, N.P. Kadzioch, D. Milius, F. Origgi, P. Plattet, MSphere 6 (2021).
date_created: 2021-05-02T22:01:28Z
date_published: 2021-04-14T00:00:00Z
date_updated: 2023-08-08T13:26:12Z
day: '14'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1128/mSphere.01024-20
external_id:
isi:
- '000663823400025'
pmid:
- '33853875'
file:
- access_level: open_access
checksum: 310748d140c8838335c1314431095898
content_type: application/pdf
creator: kschuh
date_created: 2021-05-04T12:41:38Z
date_updated: 2021-05-04T12:41:38Z
file_id: '9370'
file_name: 2021_mSphere_Gast.pdf
file_size: 3379349
relation: main_file
success: 1
file_date_updated: 2021-05-04T12:41:38Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
issue: '2'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: mSphere
publication_identifier:
eissn:
- '23795042'
publication_status: published
publisher: American Society for Microbiology
quality_controlled: '1'
scopus_import: '1'
status: public
title: Oligomerization and cell egress controlled by two microdomains of canine distemper
virus matrix protein
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2021'
...