[{"doi":"10.1038/s41467-021-23854-x","acknowledged_ssus":[{"_id":"EM-Fac"}],"language":[{"iso":"eng"}],"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"pmid":["34108481"],"isi":["000664874700014"]},"quality_controlled":"1","isi":1,"month":"06","publication_identifier":{"eissn":["2041-1723"]},"author":[{"full_name":"Prattes, Michael","first_name":"Michael","last_name":"Prattes"},{"full_name":"Grishkovskaya, Irina","first_name":"Irina","last_name":"Grishkovskaya"},{"full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau","first_name":"Victor-Valentin","id":"3661B498-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ingrid","last_name":"Rössler","full_name":"Rössler, Ingrid"},{"first_name":"Isabella","last_name":"Klein","full_name":"Klein, Isabella"},{"last_name":"Hetzmannseder","first_name":"Christina","full_name":"Hetzmannseder, Christina"},{"first_name":"Gertrude","last_name":"Zisser","full_name":"Zisser, Gertrude"},{"full_name":"Gruber, Christian C.","first_name":"Christian C.","last_name":"Gruber"},{"full_name":"Gruber, Karl","last_name":"Gruber","first_name":"Karl"},{"last_name":"Haselbach","first_name":"David","full_name":"Haselbach, David"},{"first_name":"Helmut","last_name":"Bergler","full_name":"Bergler, Helmut"}],"date_updated":"2023-08-08T14:05:26Z","date_created":"2021-06-10T14:57:45Z","volume":12,"acknowledgement":"We are deeply grateful to the late Gregor Högenauer who built the foundation for this study with his visionary work on the inhibitor diazaborine and its bacterial target. We thank Rolf Breinbauer for insightful discussions on boron chemistry. We thank Anton Meinhart and Tim Clausen for the valuable discussion of the manuscript. We are indebted to Thomas Köcher for the MS measurement of the diazaborine-ATPγS adduct. We thank the team of the VBCF for support during early phases of this work and the IST Austria Electron Microscopy Facility for providing equipment. The lab of D.H. is supported by Boehringer Ingelheim. The work was funded by FWF projects P32536 and P32977 (to H.B.).","year":"2021","pmid":1,"publication_status":"published","publisher":"Springer Nature","department":[{"_id":"EM-Fac"}],"file_date_updated":"2021-06-15T18:55:59Z","article_number":"3483","date_published":"2021-06-09T00:00:00Z","publication":"Nature Communications","citation":{"ama":"Prattes M, Grishkovskaya I, Hodirnau V-V, et al. Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. Nature Communications. 2021;12(1). doi:10.1038/s41467-021-23854-x","ista":"Prattes M, Grishkovskaya I, Hodirnau V-V, Rössler I, Klein I, Hetzmannseder C, Zisser G, Gruber CC, Gruber K, Haselbach D, Bergler H. 2021. Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. Nature Communications. 12(1), 3483.","ieee":"M. Prattes et al., “Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine,” Nature Communications, vol. 12, no. 1. Springer Nature, 2021.","apa":"Prattes, M., Grishkovskaya, I., Hodirnau, V.-V., Rössler, I., Klein, I., Hetzmannseder, C., … Bergler, H. (2021). Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-021-23854-x","mla":"Prattes, Michael, et al. “Structural Basis for Inhibition of the AAA-ATPase Drg1 by Diazaborine.” Nature Communications, vol. 12, no. 1, 3483, Springer Nature, 2021, doi:10.1038/s41467-021-23854-x.","short":"M. Prattes, I. Grishkovskaya, V.-V. Hodirnau, I. Rössler, I. Klein, C. Hetzmannseder, G. Zisser, C.C. Gruber, K. Gruber, D. Haselbach, H. Bergler, Nature Communications 12 (2021).","chicago":"Prattes, Michael, Irina Grishkovskaya, Victor-Valentin Hodirnau, Ingrid Rössler, Isabella Klein, Christina Hetzmannseder, Gertrude Zisser, et al. “Structural Basis for Inhibition of the AAA-ATPase Drg1 by Diazaborine.” Nature Communications. Springer Nature, 2021. https://doi.org/10.1038/s41467-021-23854-x."},"article_type":"original","day":"09","has_accepted_license":"1","article_processing_charge":"No","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"file":[{"creator":"cziletti","content_type":"application/pdf","file_size":3397292,"access_level":"open_access","file_name":"2021_NatureComm_Prattes.pdf","success":1,"checksum":"40fc24c1310930990b52a8ad1142ee97","date_created":"2021-06-15T18:55:59Z","date_updated":"2021-06-15T18:55:59Z","file_id":"9556","relation":"main_file"}],"oa_version":"Published Version","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9540","ddc":["570"],"title":"Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine","status":"public","intvolume":" 12","abstract":[{"lang":"eng","text":"The hexameric AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis and initiates cytoplasmic maturation of the large ribosomal subunit by releasing the shuttling maturation factor Rlp24. Drg1 monomers contain two AAA-domains (D1 and D2) that act in a concerted manner. Rlp24 release is inhibited by the drug diazaborine which blocks ATP hydrolysis in D2. The mode of inhibition was unknown. Here we show the first cryo-EM structure of Drg1 revealing the inhibitory mechanism. Diazaborine forms a covalent bond to the 2′-OH of the nucleotide in D2, explaining its specificity for this site. As a consequence, the D2 domain is locked in a rigid, inactive state, stalling the whole Drg1 hexamer. Resistance mechanisms identified include abolished drug binding and altered positioning of the nucleotide. Our results suggest nucleotide-modifying compounds as potential novel inhibitors for AAA-ATPases."}],"issue":"1","type":"journal_article"},{"article_type":"original","citation":{"ama":"Bespalov A, Bernard R, Gilis A, et al. Introduction to the EQIPD quality system. eLife. 2021;10. doi:10.7554/eLife.63294","ista":"Bespalov A, Bernard R, Gilis A, Gerlach B, Guillén J, Castagné V, Lefevre IA, Ducrey F, Monk L, Bongiovanni S, Altevogt B, Arroyo-Araujo M, Bikovski L, De Bruin N, Castaños-Vélez E, Dityatev A, Emmerich CH, Fares R, Ferland-Beckham C, Froger-Colléaux C, Gailus-Durner V, Hölter SM, Hofmann MC, Kabitzke P, Kas MJ, Kurreck C, Moser P, Pietraszek M, Popik P, Potschka H, Prado Montes De Oca E, Restivo L, Riedel G, Ritskes-Hoitinga M, Samardzic J, Schunn M, Stöger C, Voikar V, Vollert J, Wever KE, Wuyts K, Macleod MR, Dirnagl U, Steckler T. 2021. Introduction to the EQIPD quality system. eLife. 10.","ieee":"A. Bespalov et al., “Introduction to the EQIPD quality system,” eLife, vol. 10. eLife Sciences Publications, 2021.","apa":"Bespalov, A., Bernard, R., Gilis, A., Gerlach, B., Guillén, J., Castagné, V., … Steckler, T. (2021). Introduction to the EQIPD quality system. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.63294","mla":"Bespalov, Anton, et al. “Introduction to the EQIPD Quality System.” ELife, vol. 10, eLife Sciences Publications, 2021, doi:10.7554/eLife.63294.","short":"A. Bespalov, R. Bernard, A. Gilis, B. Gerlach, J. Guillén, V. Castagné, I.A. Lefevre, F. Ducrey, L. Monk, S. Bongiovanni, B. Altevogt, M. Arroyo-Araujo, L. Bikovski, N. De Bruin, E. Castaños-Vélez, A. Dityatev, C.H. Emmerich, R. Fares, C. Ferland-Beckham, C. Froger-Colléaux, V. Gailus-Durner, S.M. Hölter, M.C. Hofmann, P. Kabitzke, M.J. Kas, C. Kurreck, P. Moser, M. Pietraszek, P. Popik, H. Potschka, E. Prado Montes De Oca, L. Restivo, G. Riedel, M. Ritskes-Hoitinga, J. Samardzic, M. Schunn, C. Stöger, V. Voikar, J. Vollert, K.E. Wever, K. Wuyts, M.R. Macleod, U. Dirnagl, T. Steckler, ELife 10 (2021).","chicago":"Bespalov, Anton, René Bernard, Anja Gilis, Björn Gerlach, Javier Guillén, Vincent Castagné, Isabel A. Lefevre, et al. “Introduction to the EQIPD Quality System.” ELife. eLife Sciences Publications, 2021. https://doi.org/10.7554/eLife.63294."},"publication":"eLife","date_published":"2021-05-24T00:00:00Z","scopus_import":"1","article_processing_charge":"No","has_accepted_license":"1","day":"24","intvolume":" 10","title":"Introduction to the EQIPD quality system","ddc":["570"],"status":"public","_id":"9607","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","file":[{"file_id":"9609","relation":"main_file","success":1,"checksum":"885b746051a7a6b6e24e3d2781a48fde","date_created":"2021-06-28T11:35:30Z","date_updated":"2021-06-28T11:35:30Z","access_level":"open_access","file_name":"2021_ELife_Bespalov.pdf","creator":"asandaue","file_size":2500720,"content_type":"application/pdf"}],"type":"journal_article","abstract":[{"text":"While high risk of failure is an inherent part of developing innovative therapies, it can be reduced by adherence to evidence-based rigorous research practices. Numerous analyses conducted to date have clearly identified measures that need to be taken to improve research rigor. Supported through the European Union's Innovative Medicines Initiative, the EQIPD consortium has developed a novel preclinical research quality system that can be applied in both public and private sectors and is free for anyone to use. The EQIPD Quality System was designed to be suited to boost innovation by ensuring the generation of robust and reliable preclinical data while being lean, effective and not becoming a burden that could negatively impact the freedom to explore scientific questions. EQIPD defines research quality as the extent to which research data are fit for their intended use. Fitness, in this context, is defined by the stakeholders, who are the scientists directly involved in the research, but also their funders, sponsors, publishers, research tool manufacturers and collaboration partners such as peers in a multi-site research project. The essence of the EQIPD Quality System is the set of 18 core requirements that can be addressed flexibly, according to user-specific needs and following a user-defined trajectory. The EQIPD Quality System proposes guidance on expectations for quality-related measures, defines criteria for adequate processes (i.e., performance standards) and provides examples of how such measures can be developed and implemented. However, it does not prescribe any pre-determined solutions. EQIPD has also developed tools (for optional use) to support users in implementing the system and assessment services for those research units that successfully implement the quality system and seek formal accreditation. Building upon the feedback from users and continuous improvement, a sustainable EQIPD Quality System will ultimately serve the entire community of scientists conducting non-regulated preclinical research, by helping them generate reliable data that are fit for their intended use.","lang":"eng"}],"quality_controlled":"1","isi":1,"oa":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000661272000001"],"pmid":["34028353"]},"language":[{"iso":"eng"}],"doi":"10.7554/eLife.63294","publication_identifier":{"eissn":["2050084X"]},"month":"05","department":[{"_id":"PreCl"}],"publisher":"eLife Sciences Publications","publication_status":"published","pmid":1,"year":"2021","acknowledgement":"This project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement No 777364. This Joint Undertaking receives support from the European Union’s Horizon 2020 research and innovation programme and EFPIA. The authors are very grateful to Martin Heinrich (Abbvie, Ludwigshafen, Germany) for the exceptional IT support and programming the EQIPD Planning Tool and the Creator Tool and to Dr Shai Silberberg (NINDS, USA), Dr. Renza Roncarati (PAASP Italy) and Dr Judith Homberg (Radboud University, Nijmegen) for highly stimulating contributions to the discussions and comments on earlier versions of this manuscript. We also wish to express our thanks to Dr. Sara Stöber (concentris research management GmbH, Fürstenfeldbruck, Germany) for excellent and continuous support of this project. Creation of the EQIPD Stakeholder group was supported by Noldus Information Technology bv (Wageningen, the Netherlands).","volume":10,"date_created":"2021-06-27T22:01:49Z","date_updated":"2023-08-10T13:36:50Z","author":[{"last_name":"Bespalov","first_name":"Anton","full_name":"Bespalov, Anton"},{"last_name":"Bernard","first_name":"René","full_name":"Bernard, René"},{"full_name":"Gilis, Anja","first_name":"Anja","last_name":"Gilis"},{"full_name":"Gerlach, Björn","last_name":"Gerlach","first_name":"Björn"},{"first_name":"Javier","last_name":"Guillén","full_name":"Guillén, Javier"},{"first_name":"Vincent","last_name":"Castagné","full_name":"Castagné, Vincent"},{"full_name":"Lefevre, Isabel A.","first_name":"Isabel A.","last_name":"Lefevre"},{"full_name":"Ducrey, Fiona","last_name":"Ducrey","first_name":"Fiona"},{"last_name":"Monk","first_name":"Lee","full_name":"Monk, Lee"},{"full_name":"Bongiovanni, Sandrine","first_name":"Sandrine","last_name":"Bongiovanni"},{"full_name":"Altevogt, Bruce","last_name":"Altevogt","first_name":"Bruce"},{"full_name":"Arroyo-Araujo, María","first_name":"María","last_name":"Arroyo-Araujo"},{"last_name":"Bikovski","first_name":"Lior","full_name":"Bikovski, Lior"},{"full_name":"De Bruin, Natasja","first_name":"Natasja","last_name":"De Bruin"},{"full_name":"Castaños-Vélez, Esmeralda","last_name":"Castaños-Vélez","first_name":"Esmeralda"},{"full_name":"Dityatev, Alexander","first_name":"Alexander","last_name":"Dityatev"},{"first_name":"Christoph H.","last_name":"Emmerich","full_name":"Emmerich, Christoph H."},{"last_name":"Fares","first_name":"Raafat","full_name":"Fares, Raafat"},{"full_name":"Ferland-Beckham, Chantelle","first_name":"Chantelle","last_name":"Ferland-Beckham"},{"last_name":"Froger-Colléaux","first_name":"Christelle","full_name":"Froger-Colléaux, Christelle"},{"last_name":"Gailus-Durner","first_name":"Valerie","full_name":"Gailus-Durner, Valerie"},{"last_name":"Hölter","first_name":"Sabine M.","full_name":"Hölter, Sabine M."},{"full_name":"Hofmann, Martine Cj","first_name":"Martine Cj","last_name":"Hofmann"},{"first_name":"Patricia","last_name":"Kabitzke","full_name":"Kabitzke, Patricia"},{"first_name":"Martien Jh","last_name":"Kas","full_name":"Kas, Martien Jh"},{"first_name":"Claudia","last_name":"Kurreck","full_name":"Kurreck, Claudia"},{"full_name":"Moser, Paul","last_name":"Moser","first_name":"Paul"},{"first_name":"Malgorzata","last_name":"Pietraszek","full_name":"Pietraszek, Malgorzata"},{"full_name":"Popik, Piotr","first_name":"Piotr","last_name":"Popik"},{"last_name":"Potschka","first_name":"Heidrun","full_name":"Potschka, Heidrun"},{"full_name":"Prado Montes De Oca, Ernesto","first_name":"Ernesto","last_name":"Prado Montes De Oca"},{"full_name":"Restivo, Leonardo","last_name":"Restivo","first_name":"Leonardo"},{"last_name":"Riedel","first_name":"Gernot","full_name":"Riedel, Gernot"},{"first_name":"Merel","last_name":"Ritskes-Hoitinga","full_name":"Ritskes-Hoitinga, Merel"},{"first_name":"Janko","last_name":"Samardzic","full_name":"Samardzic, Janko"},{"last_name":"Schunn","first_name":"Michael","orcid":"0000-0003-4326-5300","id":"4272DB4A-F248-11E8-B48F-1D18A9856A87","full_name":"Schunn, Michael"},{"full_name":"Stöger, Claudia","first_name":"Claudia","last_name":"Stöger"},{"full_name":"Voikar, Vootele","last_name":"Voikar","first_name":"Vootele"},{"last_name":"Vollert","first_name":"Jan","full_name":"Vollert, Jan"},{"full_name":"Wever, Kimberley E.","last_name":"Wever","first_name":"Kimberley E."},{"last_name":"Wuyts","first_name":"Kathleen","full_name":"Wuyts, Kathleen"},{"last_name":"Macleod","first_name":"Malcolm R.","full_name":"Macleod, Malcolm R."},{"full_name":"Dirnagl, Ulrich","first_name":"Ulrich","last_name":"Dirnagl"},{"full_name":"Steckler, Thomas","first_name":"Thomas","last_name":"Steckler"}],"file_date_updated":"2021-06-28T11:35:30Z"},{"issue":"12","abstract":[{"text":"Mosaic analysis with double markers (MADM) offers one approach to visualize and concomitantly manipulate genetically defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage, single-cell morphology and physiology, genomic imprinting phenotypes, and dissection of cell-autonomous gene functions in vivo in health and disease. Yet, MADM can only be applied to <25% of all mouse genes on select chromosomes to date. To overcome this limitation, we generate transgenic mice with knocked-in MADM cassettes near the centromeres of all 19 autosomes and validate their use across organs. With this resource, >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond a proof of principle, we apply our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We find striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","file":[{"creator":"asandaue","content_type":"application/pdf","file_size":7653149,"access_level":"open_access","file_name":"2021_CellReports_Contreras.pdf","success":1,"checksum":"d49520fdcbbb5c2f883bddb67cee5d77","date_updated":"2021-06-28T14:06:24Z","date_created":"2021-06-28T14:06:24Z","file_id":"9613","relation":"main_file"}],"intvolume":" 35","ddc":["570"],"status":"public","title":"A genome-wide library of MADM mice for single-cell genetic mosaic analysis","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9603","article_processing_charge":"No","has_accepted_license":"1","day":"22","scopus_import":"1","date_published":"2021-06-22T00:00:00Z","article_type":"original","citation":{"chicago":"Contreras, Ximena, Nicole Amberg, Amarbayasgalan Davaatseren, Andi H Hansen, Johanna Sonntag, Lill Andersen, Tina Bernthaler, et al. “A Genome-Wide Library of MADM Mice for Single-Cell Genetic Mosaic Analysis.” Cell Reports. Cell Press, 2021. https://doi.org/10.1016/j.celrep.2021.109274.","short":"X. Contreras, N. Amberg, A. Davaatseren, A.H. Hansen, J. Sonntag, L. Andersen, T. Bernthaler, C. Streicher, A.-M. Heger, R.L. Johnson, L.A. Schwarz, L. Luo, T. Rülicke, S. Hippenmeyer, Cell Reports 35 (2021).","mla":"Contreras, Ximena, et al. “A Genome-Wide Library of MADM Mice for Single-Cell Genetic Mosaic Analysis.” Cell Reports, vol. 35, no. 12, 109274, Cell Press, 2021, doi:10.1016/j.celrep.2021.109274.","ieee":"X. Contreras et al., “A genome-wide library of MADM mice for single-cell genetic mosaic analysis,” Cell Reports, vol. 35, no. 12. Cell Press, 2021.","apa":"Contreras, X., Amberg, N., Davaatseren, A., Hansen, A. H., Sonntag, J., Andersen, L., … Hippenmeyer, S. (2021). A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2021.109274","ista":"Contreras X, Amberg N, Davaatseren A, Hansen AH, Sonntag J, Andersen L, Bernthaler T, Streicher C, Heger A-M, Johnson RL, Schwarz LA, Luo L, Rülicke T, Hippenmeyer S. 2021. A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Reports. 35(12), 109274.","ama":"Contreras X, Amberg N, Davaatseren A, et al. A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Reports. 2021;35(12). doi:10.1016/j.celrep.2021.109274"},"publication":"Cell Reports","ec_funded":1,"file_date_updated":"2021-06-28T14:06:24Z","article_number":"109274","volume":35,"date_created":"2021-06-27T22:01:48Z","date_updated":"2023-08-10T13:55:00Z","related_material":{"link":[{"url":"https://ist.ac.at/en/news/boost-for-mouse-genetic-analysis/","description":"News on IST Homepage","relation":"press_release"}]},"author":[{"id":"475990FE-F248-11E8-B48F-1D18A9856A87","last_name":"Contreras","first_name":"Ximena","full_name":"Contreras, Ximena"},{"last_name":"Amberg","first_name":"Nicole","orcid":"0000-0002-3183-8207","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","full_name":"Amberg, Nicole"},{"full_name":"Davaatseren, Amarbayasgalan","id":"70ADC922-B424-11E9-99E3-BA18E6697425","first_name":"Amarbayasgalan","last_name":"Davaatseren"},{"id":"38853E16-F248-11E8-B48F-1D18A9856A87","first_name":"Andi H","last_name":"Hansen","full_name":"Hansen, Andi H"},{"full_name":"Sonntag, Johanna","id":"32FE7D7C-F248-11E8-B48F-1D18A9856A87","last_name":"Sonntag","first_name":"Johanna"},{"last_name":"Andersen","first_name":"Lill","full_name":"Andersen, Lill"},{"full_name":"Bernthaler, Tina","last_name":"Bernthaler","first_name":"Tina"},{"full_name":"Streicher, Carmen","first_name":"Carmen","last_name":"Streicher","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heger, Anna-Magdalena","id":"4B76FFD2-F248-11E8-B48F-1D18A9856A87","last_name":"Heger","first_name":"Anna-Magdalena"},{"full_name":"Johnson, Randy L.","last_name":"Johnson","first_name":"Randy L."},{"full_name":"Schwarz, Lindsay A.","first_name":"Lindsay A.","last_name":"Schwarz"},{"last_name":"Luo","first_name":"Liqun","full_name":"Luo, Liqun"},{"first_name":"Thomas","last_name":"Rülicke","full_name":"Rülicke, Thomas"},{"full_name":"Hippenmeyer, Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2279-1061","first_name":"Simon","last_name":"Hippenmeyer"}],"department":[{"_id":"SiHi"},{"_id":"LoSw"},{"_id":"PreCl"}],"publisher":"Cell Press","publication_status":"published","acknowledgement":"We thank the Bioimaging, Life Science, and Pre-Clinical Facilities at IST Austria; M.P. Postiglione, C. Simbriger, K. Valoskova, C. Schwayer, T. Hussain, M. Pieber, and V. Wimmer for initial experiments, technical support, and/or assistance; R. Shigemoto for sharing iv (Dnah11 mutant) mice; and M. Sixt and all members of the Hippenmeyer lab for discussion. This work was supported by National Institutes of Health grants ( R01-NS050580 to L.L. and F32MH096361 to L.A.S.). L.L. is an investigator of HHMI. N.A. received support from FWF Firnberg-Programm ( T 1031 ). A.H.H. is a recipient of a DOC Fellowship (24812) of the Austrian Academy of Sciences . This work also received support from IST Austria institutional funds , FWF SFB F78 to S.H., the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme ( FP7/2007-2013 ) under REA grant agreement no 618444 to S.H., and the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (grant agreement no. 725780 LinPro ) to S.H.","year":"2021","publication_identifier":{"eissn":["22111247"]},"month":"06","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"doi":"10.1016/j.celrep.2021.109274","project":[{"name":"Molecular Mechanisms of Radial Neuronal Migration","_id":"2625A13E-B435-11E9-9278-68D0E5697425","grant_number":"24812"},{"call_identifier":"FP7","name":"Molecular Mechanisms of Cerebral Cortex Development","_id":"25D61E48-B435-11E9-9278-68D0E5697425","grant_number":"618444"},{"_id":"260018B0-B435-11E9-9278-68D0E5697425","grant_number":"725780","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","call_identifier":"H2020"}],"quality_controlled":"1","isi":1,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"external_id":{"isi":["000664463600016"]},"oa":1},{"author":[{"last_name":"Zisis","first_name":"Themistoklis","full_name":"Zisis, Themistoklis"},{"full_name":"Schwarz, Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz","first_name":"Jan"},{"full_name":"Balles, Miriam","last_name":"Balles","first_name":"Miriam"},{"full_name":"Kretschmer, Maibritt","last_name":"Kretschmer","first_name":"Maibritt"},{"last_name":"Nemethova","first_name":"Maria","id":"34E27F1C-F248-11E8-B48F-1D18A9856A87","full_name":"Nemethova, Maria"},{"last_name":"Chait","first_name":"Remy P","orcid":"0000-0003-0876-3187","id":"3464AE84-F248-11E8-B48F-1D18A9856A87","full_name":"Chait, Remy P"},{"full_name":"Hauschild, Robert","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"last_name":"Lange","first_name":"Janina","full_name":"Lange, Janina"},{"full_name":"Guet, Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","first_name":"Calin C","last_name":"Guet"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X"},{"first_name":"Stefan","last_name":"Zahler","full_name":"Zahler, Stefan"}],"volume":13,"date_created":"2021-08-08T22:01:28Z","date_updated":"2023-08-10T14:22:48Z","pmid":1,"acknowledgement":"We would like to thank Charlott Leu for the production of our chromium wafers, Louise Ritter for her contribution of the IF stainings in Figure 4, Shokoufeh Teymouri for her help with the Bioinert coated slides, and finally Prof. Dr. Joachim Rädler for his valuable scientific guidance.","year":"2021","publisher":"American Chemical Society","department":[{"_id":"MiSi"},{"_id":"GaTk"},{"_id":"Bio"},{"_id":"CaGu"}],"publication_status":"published","ec_funded":1,"file_date_updated":"2021-08-09T09:44:03Z","doi":"10.1021/acsami.1c09850","language":[{"iso":"eng"}],"external_id":{"isi":["000683741400026"],"pmid":["34283577"]},"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"project":[{"grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","call_identifier":"H2020"}],"isi":1,"quality_controlled":"1","publication_identifier":{"eissn":["19448252"],"issn":["19448244"]},"month":"08","oa_version":"Published Version","file":[{"relation":"main_file","file_id":"9833","date_updated":"2021-08-09T09:44:03Z","date_created":"2021-08-09T09:44:03Z","checksum":"b043a91d9f9200e467b970b692687ed3","success":1,"file_name":"2021_ACSAppliedMaterialsAndInterfaces_Zisis.pdf","access_level":"open_access","file_size":7123293,"content_type":"application/pdf","creator":"asandaue"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9822","intvolume":" 13","ddc":["620","570"],"status":"public","title":"Sequential and switchable patterning for studying cellular processes under spatiotemporal control","issue":"30","abstract":[{"text":"Attachment of adhesive molecules on cell culture surfaces to restrict cell adhesion to defined areas and shapes has been vital for the progress of in vitro research. In currently existing patterning methods, a combination of pattern properties such as stability, precision, specificity, high-throughput outcome, and spatiotemporal control is highly desirable but challenging to achieve. Here, we introduce a versatile and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent patterning step and a subsequent functionalization of the pattern via click chemistry. This two-step process is feasible on arbitrary surfaces and allows for generation of sustainable patterns and gradients. The method is validated in different biological systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining the growth and migration of cells to the designated areas. We then implement a sequential photopatterning approach by adding a second switchable patterning step, allowing for spatiotemporal control over two distinct surface patterns. As a proof of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis. Our results show that the spatiotemporal control provided by our “sequential photopatterning” system is essential for mimicking dynamic biological processes and that our innovative approach has great potential for further applications in cell science.","lang":"eng"}],"type":"journal_article","date_published":"2021-08-04T00:00:00Z","citation":{"ama":"Zisis T, Schwarz J, Balles M, et al. Sequential and switchable patterning for studying cellular processes under spatiotemporal control. ACS Applied Materials and Interfaces. 2021;13(30):35545–35560. doi:10.1021/acsami.1c09850","ista":"Zisis T, Schwarz J, Balles M, Kretschmer M, Nemethova M, Chait RP, Hauschild R, Lange J, Guet CC, Sixt MK, Zahler S. 2021. Sequential and switchable patterning for studying cellular processes under spatiotemporal control. ACS Applied Materials and Interfaces. 13(30), 35545–35560.","apa":"Zisis, T., Schwarz, J., Balles, M., Kretschmer, M., Nemethova, M., Chait, R. P., … Zahler, S. (2021). Sequential and switchable patterning for studying cellular processes under spatiotemporal control. ACS Applied Materials and Interfaces. American Chemical Society. https://doi.org/10.1021/acsami.1c09850","ieee":"T. Zisis et al., “Sequential and switchable patterning for studying cellular processes under spatiotemporal control,” ACS Applied Materials and Interfaces, vol. 13, no. 30. American Chemical Society, pp. 35545–35560, 2021.","mla":"Zisis, Themistoklis, et al. “Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.” ACS Applied Materials and Interfaces, vol. 13, no. 30, American Chemical Society, 2021, pp. 35545–35560, doi:10.1021/acsami.1c09850.","short":"T. Zisis, J. Schwarz, M. Balles, M. Kretschmer, M. Nemethova, R.P. Chait, R. Hauschild, J. Lange, C.C. Guet, M.K. Sixt, S. Zahler, ACS Applied Materials and Interfaces 13 (2021) 35545–35560.","chicago":"Zisis, Themistoklis, Jan Schwarz, Miriam Balles, Maibritt Kretschmer, Maria Nemethova, Remy P Chait, Robert Hauschild, et al. “Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.” ACS Applied Materials and Interfaces. American Chemical Society, 2021. https://doi.org/10.1021/acsami.1c09850."},"publication":"ACS Applied Materials and Interfaces","page":"35545–35560","article_type":"original","article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","day":"04","scopus_import":"1"},{"isi":1,"quality_controlled":"1","oa":1,"main_file_link":[{"url":"https://doi.org/10.1111/jmi.13041","open_access":"1"}],"external_id":{"isi":["000683702700001"]},"language":[{"iso":"eng"}],"doi":"10.1111/jmi.13041","publication_identifier":{"issn":["0022-2720"],"eissn":["1365-2818"]},"month":"08","department":[{"_id":"Bio"}],"publisher":"Wiley","publication_status":"published","year":"2021","acknowledgement":"We thank https://www.somersault1824.com/somersault18:24 BV (Leuven, Belgium) for help with Figure 1. E. C.-S. was supported by the project PPBI-POCI-01-0145-FEDER-022122, in the scope of Fundação para a Ciência e Tecnologia, Portugal (FCT) National Roadmap of Research Infrastructures. R.N. was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) Grant number Ni 451/9-1 - MIAP-Freiburg.","volume":284,"date_created":"2021-08-15T22:01:29Z","date_updated":"2023-08-11T10:30:40Z","author":[{"full_name":"Nelson, Glyn","first_name":"Glyn","last_name":"Nelson"},{"full_name":"Boehm, Ulrike","first_name":"Ulrike","last_name":"Boehm"},{"first_name":"Steve","last_name":"Bagley","full_name":"Bagley, Steve"},{"full_name":"Bajcsy, Peter","last_name":"Bajcsy","first_name":"Peter"},{"first_name":"Johanna","last_name":"Bischof","full_name":"Bischof, Johanna"},{"full_name":"Brown, Claire M.","last_name":"Brown","first_name":"Claire M."},{"full_name":"Dauphin, Aurélien","last_name":"Dauphin","first_name":"Aurélien"},{"last_name":"Dobbie","first_name":"Ian M.","full_name":"Dobbie, Ian M."},{"last_name":"Eriksson","first_name":"John E.","full_name":"Eriksson, John E."},{"full_name":"Faklaris, Orestis","first_name":"Orestis","last_name":"Faklaris"},{"first_name":"Julia","last_name":"Fernandez-Rodriguez","full_name":"Fernandez-Rodriguez, Julia"},{"last_name":"Ferrand","first_name":"Alexia","full_name":"Ferrand, Alexia"},{"full_name":"Gelman, Laurent","last_name":"Gelman","first_name":"Laurent"},{"full_name":"Gheisari, Ali","first_name":"Ali","last_name":"Gheisari"},{"last_name":"Hartmann","first_name":"Hella","full_name":"Hartmann, Hella"},{"full_name":"Kukat, Christian","first_name":"Christian","last_name":"Kukat"},{"first_name":"Alex","last_name":"Laude","full_name":"Laude, Alex"},{"last_name":"Mitkovski","first_name":"Miso","full_name":"Mitkovski, Miso"},{"last_name":"Munck","first_name":"Sebastian","full_name":"Munck, Sebastian"},{"full_name":"North, Alison J.","last_name":"North","first_name":"Alison J."},{"full_name":"Rasse, Tobias M.","first_name":"Tobias M.","last_name":"Rasse"},{"last_name":"Resch-Genger","first_name":"Ute","full_name":"Resch-Genger, Ute"},{"last_name":"Schuetz","first_name":"Lucas C.","full_name":"Schuetz, Lucas C."},{"first_name":"Arne","last_name":"Seitz","full_name":"Seitz, Arne"},{"full_name":"Strambio-De-Castillia, Caterina","last_name":"Strambio-De-Castillia","first_name":"Caterina"},{"last_name":"Swedlow","first_name":"Jason R.","full_name":"Swedlow, Jason R."},{"last_name":"Alexopoulos","first_name":"Ioannis","full_name":"Alexopoulos, Ioannis"},{"first_name":"Karin","last_name":"Aumayr","full_name":"Aumayr, Karin"},{"first_name":"Sergiy","last_name":"Avilov","full_name":"Avilov, Sergiy"},{"full_name":"Bakker, Gert Jan","last_name":"Bakker","first_name":"Gert Jan"},{"full_name":"Bammann, Rodrigo R.","last_name":"Bammann","first_name":"Rodrigo R."},{"full_name":"Bassi, Andrea","first_name":"Andrea","last_name":"Bassi"},{"full_name":"Beckert, Hannes","last_name":"Beckert","first_name":"Hannes"},{"first_name":"Sebastian","last_name":"Beer","full_name":"Beer, 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P.","last_name":"Cordelieres"},{"last_name":"Nery","first_name":"Elaine Del","full_name":"Nery, Elaine Del"},{"full_name":"Dietzel, Ralf","last_name":"Dietzel","first_name":"Ralf"},{"full_name":"Eismann, Frank","first_name":"Frank","last_name":"Eismann"},{"full_name":"Fazeli, Elnaz","first_name":"Elnaz","last_name":"Fazeli"},{"full_name":"Felscher, Andreas","first_name":"Andreas","last_name":"Felscher"},{"last_name":"Fried","first_name":"Hans","full_name":"Fried, Hans"},{"last_name":"Gaudreault","first_name":"Nathalie","full_name":"Gaudreault, Nathalie"},{"full_name":"Goh, Wah Ing","first_name":"Wah Ing","last_name":"Goh"},{"first_name":"Thomas","last_name":"Guilbert","full_name":"Guilbert, Thomas"},{"first_name":"Roland","last_name":"Hadleigh","full_name":"Hadleigh, Roland"},{"full_name":"Hemmerich, Peter","first_name":"Peter","last_name":"Hemmerich"},{"first_name":"Gerhard A.","last_name":"Holst","full_name":"Holst, Gerhard A."},{"full_name":"Itano, Michelle S.","first_name":"Michelle S.","last_name":"Itano"},{"full_name":"Jaffe, Claudia B.","first_name":"Claudia B.","last_name":"Jaffe"},{"full_name":"Jambor, Helena K.","last_name":"Jambor","first_name":"Helena K."},{"full_name":"Jarvis, Stuart C.","first_name":"Stuart C.","last_name":"Jarvis"},{"last_name":"Keppler","first_name":"Antje","full_name":"Keppler, Antje"},{"first_name":"David","last_name":"Kirchenbuechler","full_name":"Kirchenbuechler, David"},{"last_name":"Kirchner","first_name":"Marcel","full_name":"Kirchner, Marcel"},{"full_name":"Kobayashi, Norio","last_name":"Kobayashi","first_name":"Norio"},{"first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel"},{"full_name":"Kunis, Susanne","last_name":"Kunis","first_name":"Susanne"},{"first_name":"Judith","last_name":"Lacoste","full_name":"Lacoste, Judith"},{"full_name":"Marcello, Marco","last_name":"Marcello","first_name":"Marco"},{"full_name":"Martins, Gabriel G.","last_name":"Martins","first_name":"Gabriel G."},{"full_name":"Metcalf, Daniel J.","last_name":"Metcalf","first_name":"Daniel J."},{"full_name":"Mitchell, Claire A.","last_name":"Mitchell","first_name":"Claire A."},{"first_name":"Joshua","last_name":"Moore","full_name":"Moore, Joshua"},{"last_name":"Mueller","first_name":"Tobias","full_name":"Mueller, Tobias"},{"first_name":"Michael S.","last_name":"Nelson","full_name":"Nelson, Michael S."},{"first_name":"Stephen","last_name":"Ogg","full_name":"Ogg, Stephen"},{"full_name":"Onami, Shuichi","last_name":"Onami","first_name":"Shuichi"},{"full_name":"Palmer, Alexandra L.","first_name":"Alexandra L.","last_name":"Palmer"},{"last_name":"Paul-Gilloteaux","first_name":"Perrine","full_name":"Paul-Gilloteaux, Perrine"},{"full_name":"Pimentel, Jaime A.","last_name":"Pimentel","first_name":"Jaime A."},{"last_name":"Plantard","first_name":"Laure","full_name":"Plantard, Laure"},{"full_name":"Podder, Santosh","first_name":"Santosh","last_name":"Podder"},{"full_name":"Rexhepaj, Elton","last_name":"Rexhepaj","first_name":"Elton"},{"full_name":"Royon, Arnaud","last_name":"Royon","first_name":"Arnaud"},{"full_name":"Saari, Markku A.","first_name":"Markku A.","last_name":"Saari"},{"full_name":"Schapman, Damien","first_name":"Damien","last_name":"Schapman"},{"last_name":"Schoonderwoert","first_name":"Vincent","full_name":"Schoonderwoert, Vincent"},{"last_name":"Schroth-Diez","first_name":"Britta","full_name":"Schroth-Diez, Britta"},{"full_name":"Schwartz, Stanley","first_name":"Stanley","last_name":"Schwartz"},{"last_name":"Shaw","first_name":"Michael","full_name":"Shaw, Michael"},{"full_name":"Spitaler, Martin","last_name":"Spitaler","first_name":"Martin"},{"full_name":"Stoeckl, Martin T.","first_name":"Martin T.","last_name":"Stoeckl"},{"last_name":"Sudar","first_name":"Damir","full_name":"Sudar, Damir"},{"last_name":"Teillon","first_name":"Jeremie","full_name":"Teillon, Jeremie"},{"full_name":"Terjung, Stefan","last_name":"Terjung","first_name":"Stefan"},{"full_name":"Thuenauer, Roland","first_name":"Roland","last_name":"Thuenauer"},{"last_name":"Wilms","first_name":"Christian D.","full_name":"Wilms, Christian D."},{"last_name":"Wright","first_name":"Graham D.","full_name":"Wright, Graham D."},{"full_name":"Nitschke, Roland","first_name":"Roland","last_name":"Nitschke"}],"page":"56-73","article_type":"original","citation":{"ama":"Nelson G, Boehm U, Bagley S, et al. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. Journal of Microscopy. 2021;284(1):56-73. doi:10.1111/jmi.13041","ista":"Nelson G et al. 2021. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. Journal of Microscopy. 284(1), 56–73.","ieee":"G. Nelson et al., “QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy,” Journal of Microscopy, vol. 284, no. 1. Wiley, pp. 56–73, 2021.","apa":"Nelson, G., Boehm, U., Bagley, S., Bajcsy, P., Bischof, J., Brown, C. M., … Nitschke, R. (2021). QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. Journal of Microscopy. Wiley. https://doi.org/10.1111/jmi.13041","mla":"Nelson, Glyn, et al. “QUAREP-LiMi: A Community-Driven Initiative to Establish Guidelines for Quality Assessment and Reproducibility for Instruments and Images in Light Microscopy.” Journal of Microscopy, vol. 284, no. 1, Wiley, 2021, pp. 56–73, doi:10.1111/jmi.13041.","short":"G. Nelson, U. Boehm, S. Bagley, P. Bajcsy, J. Bischof, C.M. Brown, A. Dauphin, I.M. Dobbie, J.E. Eriksson, O. Faklaris, J. Fernandez-Rodriguez, A. Ferrand, L. Gelman, A. Gheisari, H. Hartmann, C. Kukat, A. Laude, M. Mitkovski, S. Munck, A.J. North, T.M. Rasse, U. Resch-Genger, L.C. Schuetz, A. Seitz, C. Strambio-De-Castillia, J.R. Swedlow, I. Alexopoulos, K. Aumayr, S. Avilov, G.J. Bakker, R.R. Bammann, A. Bassi, H. Beckert, S. Beer, Y. Belyaev, J. Bierwagen, K.A. Birngruber, M. Bosch, J. Breitlow, L.A. Cameron, J. Chalfoun, J.J. Chambers, C.L. Chen, E. Conde-Sousa, A.D. Corbett, F.P. Cordelieres, E.D. Nery, R. Dietzel, F. Eismann, E. Fazeli, A. Felscher, H. Fried, N. Gaudreault, W.I. Goh, T. Guilbert, R. Hadleigh, P. Hemmerich, G.A. Holst, M.S. Itano, C.B. Jaffe, H.K. Jambor, S.C. Jarvis, A. Keppler, D. Kirchenbuechler, M. Kirchner, N. Kobayashi, G. Krens, S. Kunis, J. Lacoste, M. Marcello, G.G. Martins, D.J. Metcalf, C.A. Mitchell, J. Moore, T. Mueller, M.S. Nelson, S. Ogg, S. Onami, A.L. Palmer, P. Paul-Gilloteaux, J.A. Pimentel, L. Plantard, S. Podder, E. Rexhepaj, A. Royon, M.A. Saari, D. Schapman, V. Schoonderwoert, B. Schroth-Diez, S. Schwartz, M. Shaw, M. Spitaler, M.T. Stoeckl, D. Sudar, J. Teillon, S. Terjung, R. Thuenauer, C.D. Wilms, G.D. Wright, R. Nitschke, Journal of Microscopy 284 (2021) 56–73.","chicago":"Nelson, Glyn, Ulrike Boehm, Steve Bagley, Peter Bajcsy, Johanna Bischof, Claire M. Brown, Aurélien Dauphin, et al. “QUAREP-LiMi: A Community-Driven Initiative to Establish Guidelines for Quality Assessment and Reproducibility for Instruments and Images in Light Microscopy.” Journal of Microscopy. Wiley, 2021. https://doi.org/10.1111/jmi.13041."},"publication":"Journal of Microscopy","date_published":"2021-08-11T00:00:00Z","scopus_import":"1","article_processing_charge":"Yes","day":"11","intvolume":" 284","status":"public","title":"QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"9911","oa_version":"Published Version","type":"journal_article","issue":"1","abstract":[{"text":"A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist's experiments, while more than half have even failed to reproduce their own experiments. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g. DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common standards and guidelines for quality assessment and reproducibility. In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper (1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; (2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers and observers of such; (3) outlines the current actions of the QUAREP-LiMi initiative and (4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.","lang":"eng"}]}]