[{"day":"23","date_updated":"2023-08-18T06:33:07Z","year":"2020","article_number":"e52067","date_published":"2020-01-23T00:00:00Z","month":"01","isi":1,"department":[{"_id":"JiFr"},{"_id":"GaTk"},{"_id":"EM-Fac"},{"_id":"SyCr"}],"_id":"7490","publication_status":"published","type":"journal_article","volume":9,"status":"public","citation":{"ama":"Narasimhan M, Johnson AJ, Prizak R, et al. Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants. eLife. 2020;9. doi:10.7554/eLife.52067","ista":"Narasimhan M, Johnson AJ, Prizak R, Kaufmann W, Tan S, Casillas Perez BE, Friml J. 2020. Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants. eLife. 9, e52067.","chicago":"Narasimhan, Madhumitha, Alexander J Johnson, Roshan Prizak, Walter Kaufmann, Shutang Tan, Barbara E Casillas Perez, and Jiří Friml. “Evolutionarily Unique Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” ELife. eLife Sciences Publications, 2020. https://doi.org/10.7554/eLife.52067.","mla":"Narasimhan, Madhumitha, et al. “Evolutionarily Unique Mechanistic Framework of Clathrin-Mediated Endocytosis in Plants.” ELife, vol. 9, e52067, eLife Sciences Publications, 2020, doi:10.7554/eLife.52067.","ieee":"M. Narasimhan et al., “Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants,” eLife, vol. 9. eLife Sciences Publications, 2020.","apa":"Narasimhan, M., Johnson, A. J., Prizak, R., Kaufmann, W., Tan, S., Casillas Perez, B. E., & Friml, J. (2020). Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.52067","short":"M. Narasimhan, A.J. Johnson, R. Prizak, W. Kaufmann, S. Tan, B.E. Casillas Perez, J. Friml, ELife 9 (2020)."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"file_date_updated":"2020-07-14T12:47:59Z","author":[{"full_name":"Narasimhan, Madhumitha","orcid":"0000-0002-8600-0671","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","first_name":"Madhumitha","last_name":"Narasimhan"},{"orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","last_name":"Johnson","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J"},{"full_name":"Prizak, Roshan","id":"4456104E-F248-11E8-B48F-1D18A9856A87","first_name":"Roshan","last_name":"Prizak"},{"id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","last_name":"Tan","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285"},{"last_name":"Casillas Perez","first_name":"Barbara E","id":"351ED2AA-F248-11E8-B48F-1D18A9856A87","full_name":"Casillas Perez, Barbara E"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"language":[{"iso":"eng"}],"intvolume":" 9","publication_identifier":{"eissn":["2050-084X"]},"publication":"eLife","doi":"10.7554/eLife.52067","scopus_import":"1","license":"https://creativecommons.org/licenses/by/4.0/","pmid":1,"external_id":{"isi":["000514104100001"],"pmid":["31971511"]},"article_processing_charge":"No","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"file":[{"file_name":"2020_eLife_Narasimhan.pdf","date_created":"2020-02-18T07:21:16Z","file_id":"7494","content_type":"application/pdf","file_size":7247468,"relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:47:59Z","creator":"dernst","checksum":"2052daa4be5019534f3a42f200a09f32"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"In plants, clathrin mediated endocytosis (CME) represents the major route for cargo internalisation from the cell surface. It has been assumed to operate in an evolutionary conserved manner as in yeast and animals. Here we report characterisation of ultrastructure, dynamics and mechanisms of plant CME as allowed by our advancement in electron microscopy and quantitative live imaging techniques. Arabidopsis CME appears to follow the constant curvature model and the bona fide CME population generates vesicles of a predominantly hexagonal-basket type; larger and with faster kinetics than in other models. Contrary to the existing paradigm, actin is dispensable for CME events at the plasma membrane but plays a unique role in collecting endocytic vesicles, sorting of internalised cargos and directional endosome movement that itself actively promote CME events. Internalized vesicles display a strongly delayed and sequential uncoating. These unique features highlight the independent evolution of the plant CME mechanism during the autonomous rise of multicellularity in eukaryotes."}],"acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"oa_version":"Published Version","quality_controlled":"1","title":"Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"eLife Sciences Publications","date_created":"2020-02-16T23:00:50Z","ec_funded":1,"ddc":["570","580"],"article_type":"original","oa":1},{"month":"09","date_published":"2020-09-01T00:00:00Z","_id":"7792","isi":1,"department":[{"_id":"NanoFab"}],"day":"01","date_updated":"2023-08-21T06:18:20Z","year":"2020","author":[{"full_name":"Taboada-Gutiérrez, Javier","first_name":"Javier","last_name":"Taboada-Gutiérrez"},{"last_name":"Álvarez-Pérez","first_name":"Gonzalo","full_name":"Álvarez-Pérez, Gonzalo"},{"first_name":"Jiahua","last_name":"Duan","full_name":"Duan, Jiahua"},{"last_name":"Ma","first_name":"Weiliang","full_name":"Ma, Weiliang"},{"full_name":"Crowley, Kyle","last_name":"Crowley","first_name":"Kyle"},{"full_name":"Prieto Gonzalez, Ivan","orcid":"0000-0002-7370-5357","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Ivan","last_name":"Prieto Gonzalez"},{"full_name":"Bylinkin, Andrei","first_name":"Andrei","last_name":"Bylinkin"},{"full_name":"Autore, Marta","last_name":"Autore","first_name":"Marta"},{"first_name":"Halyna","last_name":"Volkova","full_name":"Volkova, Halyna"},{"first_name":"Kenta","last_name":"Kimura","full_name":"Kimura, Kenta"},{"full_name":"Kimura, Tsuyoshi","first_name":"Tsuyoshi","last_name":"Kimura"},{"full_name":"Berger, M. H.","first_name":"M. H.","last_name":"Berger"},{"last_name":"Li","first_name":"Shaojuan","full_name":"Li, Shaojuan"},{"full_name":"Bao, Qiaoliang","first_name":"Qiaoliang","last_name":"Bao"},{"last_name":"Gao","first_name":"Xuan P.A.","full_name":"Gao, Xuan P.A."},{"full_name":"Errea, Ion","last_name":"Errea","first_name":"Ion"},{"full_name":"Nikitin, Alexey Y.","last_name":"Nikitin","first_name":"Alexey Y."},{"last_name":"Hillenbrand","first_name":"Rainer","full_name":"Hillenbrand, Rainer"},{"full_name":"Martín-Sánchez, Javier","first_name":"Javier","last_name":"Martín-Sánchez"},{"last_name":"Alonso-González","first_name":"Pablo","full_name":"Alonso-González, Pablo"}],"publication_identifier":{"eissn":["14764660"],"issn":["14761122"]},"intvolume":" 19","language":[{"iso":"eng"}],"acknowledgement":"J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa Program from the Government of the Principality of Asturias (nos. PA-18-PF-BP17-126 and PA-20-PF-BP19-053, respectively). J.M.-S. acknowledges finantial support from the Clarín Programme from the Government of the Principality of Asturias and a Marie Curie-COFUND grant (PA-18-ACB17-29) and the Ramón y Cajal Program from the Government of Spain (RYC2018-026196-I). K.C., X.P.A.G., H.V. and M.H.B. acknowledge the Air Force Office of Scientific Research (AFOSR) grant no. FA 9550-18-1-0030 for funding support. I.E. acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (grant no. FIS2016-76617-P). A.Y.N. acknowledges the Spanish Ministry of Science, Innovation and Universities (national project no. MAT2017-88358-C3-3-R) and the Basque Government (grant no. IT1164-19). Q.B. acknowledges the support from Australian Research Council (grant nos. FT150100450, IH150100006 and CE170100039). R.H. acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness (national project RTI2018-094830-B-100 and the Project MDM-2016-0618 of the María de Maeztu Units of Excellence Program) and the Basque Goverment (grant no. IT1164-19). P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA.","doi":"10.1038/s41563-020-0665-0","publication":"Nature Materials","publication_status":"published","status":"public","volume":19,"type":"journal_article","citation":{"ama":"Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, et al. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. Nature Materials. 2020;19:964–968. doi:10.1038/s41563-020-0665-0","ista":"Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, Ma W, Crowley K, Prieto Gonzalez I, Bylinkin A, Autore M, Volkova H, Kimura K, Kimura T, Berger MH, Li S, Bao Q, Gao XPA, Errea I, Nikitin AY, Hillenbrand R, Martín-Sánchez J, Alonso-González P. 2020. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. Nature Materials. 19, 964–968.","mla":"Taboada-Gutiérrez, Javier, et al. “Broad Spectral Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.” Nature Materials, vol. 19, Springer Nature, 2020, pp. 964–968, doi:10.1038/s41563-020-0665-0.","chicago":"Taboada-Gutiérrez, Javier, Gonzalo Álvarez-Pérez, Jiahua Duan, Weiliang Ma, Kyle Crowley, Ivan Prieto Gonzalez, Andrei Bylinkin, et al. “Broad Spectral Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.” Nature Materials. Springer Nature, 2020. https://doi.org/10.1038/s41563-020-0665-0.","ieee":"J. Taboada-Gutiérrez et al., “Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation,” Nature Materials, vol. 19. Springer Nature, pp. 964–968, 2020.","short":"J. Taboada-Gutiérrez, G. Álvarez-Pérez, J. Duan, W. Ma, K. Crowley, I. Prieto Gonzalez, A. Bylinkin, M. Autore, H. Volkova, K. Kimura, T. Kimura, M.H. Berger, S. Li, Q. Bao, X.P.A. Gao, I. Errea, A.Y. Nikitin, R. Hillenbrand, J. Martín-Sánchez, P. Alonso-González, Nature Materials 19 (2020) 964–968.","apa":"Taboada-Gutiérrez, J., Álvarez-Pérez, G., Duan, J., Ma, W., Crowley, K., Prieto Gonzalez, I., … Alonso-González, P. (2020). Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. Nature Materials. Springer Nature. https://doi.org/10.1038/s41563-020-0665-0"},"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Phonon polaritons—light coupled to lattice vibrations—in polar van der Waals crystals are promising candidates for controlling the flow of energy on the nanoscale due to their strong field confinement, anisotropic propagation and ultra-long lifetime in the picosecond range1,2,3,4,5. However, the lack of tunability of their narrow and material-specific spectral range—the Reststrahlen band—severely limits their technological implementation. Here, we demonstrate that intercalation of Na atoms in the van der Waals semiconductor α-V2O5 enables a broad spectral shift of Reststrahlen bands, and that the phonon polaritons excited show ultra-low losses (lifetime of 4 ± 1 ps), similar to phonon polaritons in a non-intercalated crystal (lifetime of 6 ± 1 ps). We expect our intercalation method to be applicable to other van der Waals crystals, opening the door for the use of phonon polaritons in broad spectral bands in the mid-infrared domain."}],"page":"964–968","scopus_import":"1","external_id":{"pmid":["32284598"],"isi":["000526218500004"]},"pmid":1,"date_created":"2020-05-03T22:00:49Z","article_type":"original","quality_controlled":"1","oa_version":"None","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Springer Nature","title":"Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation"},{"department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"isi":1,"_id":"7875","article_number":"e201907154","date_published":"2020-06-01T00:00:00Z","month":"06","date_updated":"2023-08-21T06:28:17Z","year":"2020","day":"01","language":[{"iso":"eng"}],"intvolume":" 219","publication_identifier":{"eissn":["1540-8140"]},"publication":"The Journal of Cell Biology","doi":"10.1083/jcb.201907154","acknowledgement":"The authors thank the Scientific Service Units (Life Sciences, Bioimaging, Preclinical) of the Institute of Science and Technology Austria for excellent support. This work was funded by the European Research Council (ERC StG 281556 and CoG 724373), two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20 to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O. Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734) and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014) co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European Funds for Social and Regional Development.","file_date_updated":"2020-11-24T13:25:13Z","author":[{"last_name":"Kopf","first_name":"Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja"},{"orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg","last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","first_name":"Jörg"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"last_name":"Girkontaite","first_name":"Irute","full_name":"Girkontaite, Irute"},{"last_name":"Tedford","first_name":"Kerry","full_name":"Tedford, Kerry"},{"last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"first_name":"Oliver","last_name":"Thorn-Seshold","full_name":"Thorn-Seshold, Oliver"},{"full_name":"Trauner, Dirk","last_name":"Trauner","first_name":"Dirk","id":"E8F27F48-3EBA-11E9-92A1-B709E6697425"},{"full_name":"Häcker, Hans","first_name":"Hans","last_name":"Häcker"},{"full_name":"Fischer, Klaus Dieter","last_name":"Fischer","first_name":"Klaus Dieter"},{"last_name":"Kiermaier","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0001-6165-5738","full_name":"Kiermaier, Eva"},{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"chicago":"Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” The Journal of Cell Biology. Rockefeller University Press, 2020. https://doi.org/10.1083/jcb.201907154.","mla":"Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” The Journal of Cell Biology, vol. 219, no. 6, e201907154, Rockefeller University Press, 2020, doi:10.1083/jcb.201907154.","ama":"Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 2020;219(6). doi:10.1083/jcb.201907154","ista":"Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 219(6), e201907154.","short":"A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin, O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt, The Journal of Cell Biology 219 (2020).","apa":"Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin, J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.201907154","ieee":"A. Kopf et al., “Microtubules control cellular shape and coherence in amoeboid migrating cells,” The Journal of Cell Biology, vol. 219, no. 6. Rockefeller University Press, 2020."},"publication_status":"published","volume":219,"type":"journal_article","status":"public","article_processing_charge":"No","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556"},{"call_identifier":"H2020","name":"Cellular navigation along spatial gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373"},{"name":"Mechanical adaptation of lamellipodial actin","call_identifier":"FWF","grant_number":"P29911","_id":"26018E70-B435-11E9-9278-68D0E5697425"},{"name":"Nano-Analytics of Cellular Systems","call_identifier":"FWF","_id":"252C3B08-B435-11E9-9278-68D0E5697425","grant_number":"W 1250-B20"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"},{"grant_number":"ALTF 1396-2014","_id":"25A48D24-B435-11E9-9278-68D0E5697425","name":"Molecular and system level view of immune cell migration"}],"file":[{"content_type":"application/pdf","file_id":"8801","success":1,"file_name":"2020_JCellBiol_Kopf.pdf","date_created":"2020-11-24T13:25:13Z","relation":"main_file","access_level":"open_access","file_size":7536712,"checksum":"cb0b9c77842ae1214caade7b77e4d82d","creator":"dernst","date_updated":"2020-11-24T13:25:13Z"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"Cells navigating through complex tissues face a fundamental challenge: while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion: (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device: they sense cell shape and control actomyosin retraction to sustain cellular coherence."}],"pmid":1,"external_id":{"pmid":["32379884"],"isi":["000538141100020"]},"scopus_import":"1","ddc":["570"],"article_type":"original","oa":1,"date_created":"2020-05-24T22:00:56Z","ec_funded":1,"issue":"6","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"}],"oa_version":"Published Version","quality_controlled":"1","title":"Microtubules control cellular shape and coherence in amoeboid migrating cells","publisher":"Rockefeller University Press","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8"},{"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"mla":"Schauer, Alexandra, et al. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.” ELife, vol. 9, e55190, eLife Sciences Publications, 2020, doi:10.7554/elife.55190.","chicago":"Schauer, Alexandra, Diana C Nunes Pinheiro, Robert Hauschild, and Carl-Philipp J Heisenberg. “Zebrafish Embryonic Explants Undergo Genetically Encoded Self-Assembly.” ELife. eLife Sciences Publications, 2020. https://doi.org/10.7554/elife.55190.","ama":"Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. Zebrafish embryonic explants undergo genetically encoded self-assembly. eLife. 2020;9. doi:10.7554/elife.55190","ista":"Schauer A, Nunes Pinheiro DC, Hauschild R, Heisenberg C-PJ. 2020. Zebrafish embryonic explants undergo genetically encoded self-assembly. eLife. 9, e55190.","apa":"Schauer, A., Nunes Pinheiro, D. C., Hauschild, R., & Heisenberg, C.-P. J. (2020). Zebrafish embryonic explants undergo genetically encoded self-assembly. ELife. eLife Sciences Publications. https://doi.org/10.7554/elife.55190","short":"A. Schauer, D.C. Nunes Pinheiro, R. Hauschild, C.-P.J. Heisenberg, ELife 9 (2020).","ieee":"A. Schauer, D. C. Nunes Pinheiro, R. Hauschild, and C.-P. J. Heisenberg, “Zebrafish embryonic explants undergo genetically encoded self-assembly,” eLife, vol. 9. eLife Sciences Publications, 2020."},"publication_status":"published","volume":9,"type":"journal_article","status":"public","language":[{"iso":"eng"}],"intvolume":" 9","publication_identifier":{"issn":["2050-084X"]},"publication":"eLife","doi":"10.7554/elife.55190","file_date_updated":"2020-07-14T12:48:04Z","author":[{"orcid":"0000-0001-7659-9142","full_name":"Schauer, Alexandra","last_name":"Schauer","first_name":"Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Nunes Pinheiro, Diana C","orcid":"0000-0003-4333-7503","first_name":"Diana C","id":"2E839F16-F248-11E8-B48F-1D18A9856A87","last_name":"Nunes Pinheiro"},{"last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"date_updated":"2023-08-21T06:25:49Z","year":"2020","related_material":{"record":[{"status":"public","id":"12891","relation":"dissertation_contains"}]},"day":"06","department":[{"_id":"CaHe"},{"_id":"Bio"}],"isi":1,"_id":"7888","article_number":"e55190","date_published":"2020-04-06T00:00:00Z","month":"04","quality_controlled":"1","oa_version":"Published Version","title":"Zebrafish embryonic explants undergo genetically encoded self-assembly","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"eLife Sciences Publications","ddc":["570"],"article_type":"original","oa":1,"date_created":"2020-05-25T15:01:40Z","ec_funded":1,"pmid":1,"external_id":{"pmid":["32250246"],"isi":["000531544400001"]},"scopus_import":"1","article_processing_charge":"No","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"},{"name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","grant_number":"25239","_id":"26B1E39C-B435-11E9-9278-68D0E5697425"},{"_id":"26520D1E-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"},{"name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation","grant_number":"LT000429","_id":"266BC5CE-B435-11E9-9278-68D0E5697425"}],"file":[{"access_level":"open_access","relation":"main_file","file_size":7744848,"creator":"dernst","checksum":"f6aad884cf706846ae9357fcd728f8b5","date_updated":"2020-07-14T12:48:04Z","file_name":"2020_eLife_Schauer.pdf","date_created":"2020-05-25T15:15:43Z","content_type":"application/pdf","file_id":"7890"}],"has_accepted_license":"1","abstract":[{"text":"Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this remarkable self-organization capacity with classical experiments demonstrating key roles for extrinsic biases by maternal factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm anlage. Importantly, explant organization requires polarized inheritance of maternal factors from dorsal-marginal regions of the blastoderm. Moreover, induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels, is highly variable in explants, reminiscent of embryos with reduced Nodal signals from the extraembryonic tissues. Together, these data suggest that zebrafish explants do not undergo bona fide self-organization, but rather display features of genetically encoded self-assembly, where intrinsic genetic programs control the emergence of order.","lang":"eng"}]},{"day":"01","date_updated":"2023-08-21T06:28:52Z","year":"2020","date_published":"2020-06-01T00:00:00Z","month":"06","isi":1,"department":[{"_id":"Bio"}],"_id":"7864","publication_status":"published","type":"journal_article","volume":20,"status":"public","citation":{"ieee":"J. Singer, J. Singer, and E. Jensen-Jarolim, “Precision medicine in clinical oncology: the journey from IgG antibody to IgE,” Current opinion in allergy and clinical immunology, vol. 20, no. 3. Wolters Kluwer, pp. 282–289, 2020.","apa":"Singer, J., Singer, J., & Jensen-Jarolim, E. (2020). Precision medicine in clinical oncology: the journey from IgG antibody to IgE. Current Opinion in Allergy and Clinical Immunology. Wolters Kluwer. https://doi.org/10.1097/ACI.0000000000000637","short":"J. Singer, J. Singer, E. Jensen-Jarolim, Current Opinion in Allergy and Clinical Immunology 20 (2020) 282–289.","ista":"Singer J, Singer J, Jensen-Jarolim E. 2020. Precision medicine in clinical oncology: the journey from IgG antibody to IgE. Current opinion in allergy and clinical immunology. 20(3), 282–289.","ama":"Singer J, Singer J, Jensen-Jarolim E. Precision medicine in clinical oncology: the journey from IgG antibody to IgE. Current opinion in allergy and clinical immunology. 2020;20(3):282-289. doi:10.1097/ACI.0000000000000637","mla":"Singer, Judit, et al. “Precision Medicine in Clinical Oncology: The Journey from IgG Antibody to IgE.” Current Opinion in Allergy and Clinical Immunology, vol. 20, no. 3, Wolters Kluwer, 2020, pp. 282–89, doi:10.1097/ACI.0000000000000637.","chicago":"Singer, Judit, Josef Singer, and Erika Jensen-Jarolim. “Precision Medicine in Clinical Oncology: The Journey from IgG Antibody to IgE.” Current Opinion in Allergy and Clinical Immunology. Wolters Kluwer, 2020. https://doi.org/10.1097/ACI.0000000000000637."},"author":[{"full_name":"Singer, Judit","orcid":"0000-0002-8777-3502","id":"36432834-F248-11E8-B48F-1D18A9856A87","first_name":"Judit","last_name":"Singer"},{"full_name":"Singer, Josef","first_name":"Josef","last_name":"Singer"},{"last_name":"Jensen-Jarolim","first_name":"Erika","full_name":"Jensen-Jarolim, Erika"}],"language":[{"iso":"eng"}],"intvolume":" 20","publication_identifier":{"eissn":["14736322"]},"publication":"Current opinion in allergy and clinical immunology","doi":"10.1097/ACI.0000000000000637","scopus_import":"1","external_id":{"isi":["000561358300010"]},"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Purpose of review: Cancer is one of the leading causes of death and the incidence rates are constantly rising. The heterogeneity of tumors poses a big challenge for the treatment of the disease and natural antibodies additionally affect disease progression. The introduction of engineered mAbs for anticancer immunotherapies has substantially improved progression-free and overall survival of cancer patients, but little efforts have been made to exploit other antibody isotypes than IgG.\r\nRecent findings: In order to improve these therapies, ‘next-generation antibodies’ were engineered to enhance a specific feature of classical antibodies and form a group of highly effective and precise therapy compounds. Advanced antibody approaches include among others antibody-drug conjugates, glyco-engineered and Fc-engineered antibodies, antibody fragments, radioimmunotherapy compounds, bispecific antibodies and alternative (non-IgG) immunoglobulin classes, especially IgE.\r\nSummary: The current review describes solutions for the needs of next-generation antibody therapies through different approaches. Careful selection of the best-suited engineering methodology is a key factor in developing personalized, more specific and more efficient mAbs against cancer to improve the outcomes of cancer patients. We highlight here the large evidence of IgE exploiting a highly cytotoxic effector arm as potential next-generation anticancer immunotherapy."}],"page":"282-289","quality_controlled":"1","oa_version":"None","title":"Precision medicine in clinical oncology: the journey from IgG antibody to IgE","publisher":"Wolters Kluwer","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"3","date_created":"2020-05-17T22:00:44Z","article_type":"original"},{"publication_identifier":{"issn":["0896-6273"]},"intvolume":" 107","language":[{"iso":"eng"}],"acknowledgement":"This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement 692692, P.J.) and the Fond zur Förderung der Wissenschaftlichen Forschung (Z 312-B27, Wittgenstein award, P.J.). We thank Gyorgy Buzsáki, Jozsef Csicsvari, Juan Ramirez Villegas, and Federico Stella for commenting on earlier versions of this manuscript. We also thank Katie Bittner, Michael Brecht, Albert Lee, Jeffery Magee, and Alejandro Pernía-Andrade for sharing expertise in in vivo patch-clamp recording. We are grateful to Florian Marr for cell labeling, cell reconstruction, and technical assistance; Ben Suter for helpful discussions; Christina Altmutter for technical support; Eleftheria Kralli-Beller for manuscript editing; and Todor Asenov (Machine Shop) for device construction. We also thank the Scientific Service Units (SSUs) of IST Austria (Machine Shop, Scientific Computing, and Preclinical Facility) for efficient support.","doi":"10.1016/j.neuron.2020.07.006","publication":"Neuron","author":[{"full_name":"Zhang, Xiaomin","last_name":"Zhang","id":"423EC9C2-F248-11E8-B48F-1D18A9856A87","first_name":"Xiaomin"},{"first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2020-12-04T09:29:21Z","citation":{"mla":"Zhang, Xiaomin, et al. “Selective Routing of Spatial Information Flow from Input to Output in Hippocampal Granule Cells.” Neuron, vol. 107, no. 6, Elsevier, 2020, pp. 1212–25, doi:10.1016/j.neuron.2020.07.006.","chicago":"Zhang, Xiaomin, Alois Schlögl, and Peter M Jonas. “Selective Routing of Spatial Information Flow from Input to Output in Hippocampal Granule Cells.” Neuron. Elsevier, 2020. https://doi.org/10.1016/j.neuron.2020.07.006.","ama":"Zhang X, Schlögl A, Jonas PM. Selective routing of spatial information flow from input to output in hippocampal granule cells. Neuron. 2020;107(6):1212-1225. doi:10.1016/j.neuron.2020.07.006","ista":"Zhang X, Schlögl A, Jonas PM. 2020. Selective routing of spatial information flow from input to output in hippocampal granule cells. Neuron. 107(6), 1212–1225.","short":"X. Zhang, A. Schlögl, P.M. Jonas, Neuron 107 (2020) 1212–1225.","apa":"Zhang, X., Schlögl, A., & Jonas, P. M. (2020). Selective routing of spatial information flow from input to output in hippocampal granule cells. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2020.07.006","ieee":"X. Zhang, A. Schlögl, and P. M. Jonas, “Selective routing of spatial information flow from input to output in hippocampal granule cells,” Neuron, vol. 107, no. 6. Elsevier, pp. 1212–1225, 2020."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"publication_status":"published","status":"public","volume":107,"type":"journal_article","_id":"8261","isi":1,"department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"month":"09","date_published":"2020-09-23T00:00:00Z","date_updated":"2023-08-22T08:30:55Z","year":"2020","related_material":{"link":[{"relation":"press_release","description":"News on IST Website","url":"https://ist.ac.at/en/news/the-bouncer-in-the-brain/"}]},"day":"23","oa":1,"article_type":"original","ddc":["570"],"ec_funded":1,"date_created":"2020-08-14T09:36:05Z","issue":"6","oa_version":"Published Version","quality_controlled":"1","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"ScienComp"},{"_id":"PreCl"}],"publisher":"Elsevier","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Selective routing of spatial information flow from input to output in hippocampal granule cells","project":[{"name":"Biophysics and circuit function of a giant cortical glumatergic synapse","call_identifier":"H2020","grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"The Wittgenstein Prize","_id":"25C5A090-B435-11E9-9278-68D0E5697425","grant_number":"Z00312"}],"article_processing_charge":"No","file":[{"success":1,"content_type":"application/pdf","file_id":"8920","file_name":"2020_Neuron_Zhang.pdf","date_created":"2020-12-04T09:29:21Z","date_updated":"2020-12-04T09:29:21Z","checksum":"44a5960fc083a4cb3488d22224859fdc","creator":"dernst","file_size":3011120,"access_level":"open_access","relation":"main_file"}],"page":"1212-1225","has_accepted_license":"1","abstract":[{"text":"Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal CA3 region, but how they process spatial information remains enigmatic. To examine the role of GCs in spatial coding, we measured excitatory postsynaptic potentials (EPSPs) and action potentials (APs) in head-fixed mice running on a linear belt. Intracellular recording from morphologically identified GCs revealed that most cells were active, but activity level varied over a wide range. Whereas only ∼5% of GCs showed spatially tuned spiking, ∼50% received spatially tuned input. Thus, the GC population broadly encodes spatial information, but only a subset relays this information to the CA3 network. Fourier analysis indicated that GCs received conjunctive place-grid-like synaptic input, suggesting code conversion in single neurons. GC firing was correlated with dendritic complexity and intrinsic excitability, but not extrinsic excitatory input or dendritic cable properties. Thus, functional maturation may control input-output transformation and spatial code conversion.","lang":"eng"}],"external_id":{"isi":["000579698700009"],"pmid":["32763145"]},"pmid":1,"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/"},{"_id":"8597","department":[{"_id":"NanoFab"}],"isi":1,"month":"09","date_published":"2020-09-23T00:00:00Z","article_number":"065005","year":"2020","date_updated":"2023-08-22T09:53:29Z","day":"23","doi":"10.1088/1478-3975/abb2db","acknowledgement":"I would especially like to thank Michael Sixt for encouraging me to think about these problems while working at home due to restrictions in place. I want to thank Nick Barton, Katka Bodova, Matthew Robinson, Simon Rella, Federico Sau, Ivan Prieto, and Pradeep Kumar for useful discussions.","publication":"Physical Biology","publication_identifier":{"eissn":["14783975"]},"language":[{"iso":"eng"}],"intvolume":" 17","author":[{"orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack"}],"file_date_updated":"2020-10-05T13:53:59Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"mla":"Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of COVID-19 Pandemic Progression Worldwide.” Physical Biology, vol. 17, no. 6, 065005, IOP Publishing, 2020, doi:10.1088/1478-3975/abb2db.","chicago":"Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of COVID-19 Pandemic Progression Worldwide.” Physical Biology. IOP Publishing, 2020. https://doi.org/10.1088/1478-3975/abb2db.","ama":"Merrin J. Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. Physical Biology. 2020;17(6). doi:10.1088/1478-3975/abb2db","ista":"Merrin J. 2020. Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. Physical Biology. 17(6), 065005.","short":"J. Merrin, Physical Biology 17 (2020).","apa":"Merrin, J. (2020). Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. Physical Biology. IOP Publishing. https://doi.org/10.1088/1478-3975/abb2db","ieee":"J. Merrin, “Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide,” Physical Biology, vol. 17, no. 6. IOP Publishing, 2020."},"status":"public","volume":17,"type":"journal_article","publication_status":"published","abstract":[{"text":"Error analysis and data visualization of positive COVID-19 cases in 27 countries have been performed up to August 8, 2020. This survey generally observes a progression from early exponential growth transitioning to an intermediate power-law growth phase, as recently suggested by Ziff and Ziff. The occurrence of logistic growth after the power-law phase with lockdowns or social distancing may be described as an effect of avoidance. A visualization of the power-law growth exponent over short time windows is qualitatively similar to the Bhatia visualization for pandemic progression. Visualizations like these can indicate the onset of second waves and may influence social policy.","lang":"eng"}],"has_accepted_license":"1","file":[{"date_updated":"2020-10-05T13:53:59Z","checksum":"fec9bdd355ed349f09990faab20838a7","creator":"dernst","file_size":1667111,"access_level":"open_access","relation":"main_file","date_created":"2020-10-05T13:53:59Z","file_name":"2020_PhysBio_Merrin.pdf","success":1,"file_id":"8609","content_type":"application/pdf"}],"article_processing_charge":"Yes (via OA deal)","external_id":{"isi":["000575539700001"]},"scopus_import":"1","oa":1,"ddc":["510","570"],"article_type":"original","date_created":"2020-10-04T22:01:35Z","issue":"6","publisher":"IOP Publishing","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide","oa_version":"Published Version","quality_controlled":"1"},{"file_date_updated":"2020-11-09T07:56:24Z","author":[{"last_name":"Schulte","first_name":"Linda","full_name":"Schulte, Linda"},{"first_name":"Jiafei","last_name":"Mao","full_name":"Mao, Jiafei"},{"first_name":"Julian","last_name":"Reitz","full_name":"Reitz, Julian"},{"last_name":"Sreeramulu","first_name":"Sridhar","full_name":"Sreeramulu, Sridhar"},{"last_name":"Kudlinzki","first_name":"Denis","full_name":"Kudlinzki, Denis"},{"full_name":"Hodirnau, Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87","first_name":"Victor-Valentin"},{"first_name":"Jakob","last_name":"Meier-Credo","full_name":"Meier-Credo, Jakob"},{"full_name":"Saxena, Krishna","last_name":"Saxena","first_name":"Krishna"},{"first_name":"Florian","last_name":"Buhr","full_name":"Buhr, Florian"},{"full_name":"Langer, Julian D.","last_name":"Langer","first_name":"Julian D."},{"full_name":"Blackledge, Martin","first_name":"Martin","last_name":"Blackledge"},{"full_name":"Frangakis, Achilleas S.","first_name":"Achilleas S.","last_name":"Frangakis"},{"last_name":"Glaubitz","first_name":"Clemens","full_name":"Glaubitz, Clemens"},{"full_name":"Schwalbe, Harald","first_name":"Harald","last_name":"Schwalbe"}],"keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"intvolume":" 11","language":[{"iso":"eng"}],"publication_identifier":{"issn":["2041-1723"]},"publication":"Nature Communications","acknowledgement":"We acknowledge help from Anja Seybert, Margot Frangakis, Diana Grewe, Mikhail Eltsov, Utz Ermel, and Shintaro Aibara. The work was supported by Deutsche Forschungsgemeinschaft in the CLiC graduate school. Work at the Center for Biomolecular Magnetic Resonance (BMRZ) is supported by the German state of Hesse. The work at BMRZ has been supported by the state of Hesse. L.S. has been supported by the DFG graduate college: CLiC.","doi":"10.1038/s41467-020-19372-x","publication_status":"published","volume":11,"type":"journal_article","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"chicago":"Schulte, Linda, Jiafei Mao, Julian Reitz, Sridhar Sreeramulu, Denis Kudlinzki, Victor-Valentin Hodirnau, Jakob Meier-Credo, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal Exit Tunnel.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-19372-x.","mla":"Schulte, Linda, et al. “Cysteine Oxidation and Disulfide Formation in the Ribosomal Exit Tunnel.” Nature Communications, vol. 11, 5569, Springer Nature, 2020, doi:10.1038/s41467-020-19372-x.","ama":"Schulte L, Mao J, Reitz J, et al. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. Nature Communications. 2020;11. doi:10.1038/s41467-020-19372-x","ista":"Schulte L, Mao J, Reitz J, Sreeramulu S, Kudlinzki D, Hodirnau V-V, Meier-Credo J, Saxena K, Buhr F, Langer JD, Blackledge M, Frangakis AS, Glaubitz C, Schwalbe H. 2020. Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. Nature Communications. 11, 5569.","short":"L. Schulte, J. Mao, J. Reitz, S. Sreeramulu, D. Kudlinzki, V.-V. Hodirnau, J. Meier-Credo, K. Saxena, F. Buhr, J.D. Langer, M. Blackledge, A.S. Frangakis, C. Glaubitz, H. Schwalbe, Nature Communications 11 (2020).","apa":"Schulte, L., Mao, J., Reitz, J., Sreeramulu, S., Kudlinzki, D., Hodirnau, V.-V., … Schwalbe, H. (2020). Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-19372-x","ieee":"L. Schulte et al., “Cysteine oxidation and disulfide formation in the ribosomal exit tunnel,” Nature Communications, vol. 11. Springer Nature, 2020."},"article_number":"5569","date_published":"2020-11-04T00:00:00Z","month":"11","isi":1,"department":[{"_id":"EM-Fac"}],"_id":"8744","day":"04","date_updated":"2023-08-22T12:36:07Z","year":"2020","date_created":"2020-11-09T07:49:36Z","article_type":"original","ddc":["570"],"oa":1,"quality_controlled":"1","oa_version":"Published Version","title":"Cysteine oxidation and disulfide formation in the ribosomal exit tunnel","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Springer Nature","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Understanding the conformational sampling of translation-arrested ribosome nascent chain complexes is key to understand co-translational folding. Up to now, coupling of cysteine oxidation, disulfide bond formation and structure formation in nascent chains has remained elusive. Here, we investigate the eye-lens protein γB-crystallin in the ribosomal exit tunnel. Using mass spectrometry, theoretical simulations, dynamic nuclear polarization-enhanced solid-state nuclear magnetic resonance and cryo-electron microscopy, we show that thiol groups of cysteine residues undergo S-glutathionylation and S-nitrosylation and form non-native disulfide bonds. Thus, covalent modification chemistry occurs already prior to nascent chain release as the ribosome exit tunnel provides sufficient space even for disulfide bond formation which can guide protein folding."}],"file":[{"file_size":1670898,"access_level":"open_access","relation":"main_file","date_updated":"2020-11-09T07:56:24Z","creator":"dernst","checksum":"b2688f0347e69e6629bba582077278c5","date_created":"2020-11-09T07:56:24Z","file_name":"2020_NatureComm_Schulte.pdf","file_id":"8745","content_type":"application/pdf","success":1}],"has_accepted_license":"1","scopus_import":"1","external_id":{"isi":["000592028600001"]}},{"date_created":"2020-11-22T23:01:23Z","ec_funded":1,"article_type":"original","ddc":["570"],"oa":1,"quality_controlled":"1","oa_version":"Published Version","title":"Vascular surveillance by haptotactic blood platelets in inflammation and infection","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Springer Nature","article_processing_charge":"No","project":[{"grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells"}],"file":[{"relation":"main_file","access_level":"open_access","file_size":7035340,"creator":"dernst","checksum":"485b7b6cf30198ba0ce126491a28f125","date_updated":"2020-11-23T13:29:49Z","date_created":"2020-11-23T13:29:49Z","file_name":"2020_NatureComm_Nicolai.pdf","content_type":"application/pdf","file_id":"8798","success":1}],"abstract":[{"text":"Breakdown of vascular barriers is a major complication of inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play a crucial role in inflammation. While spatio-temporal dynamics of clot formation are well characterized, the cell-biological mechanisms of platelet recruitment to inflammatory micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia formation as a prominent morphological feature of immune-responsive platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the inflamed vasculature and to directionally spread, to polarize and to govern haptotactic migration along gradients of the adhesive ligand. Platelet-specific abrogation of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions, thus impairing vascular sealing and provoking inflammatory microbleeding. During infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination, rendering platelets gate-keepers of the inflamed microvasculature. Consequently, these findings identify haptotaxis as a key effector function of immune-responsive platelets.","lang":"eng"}],"has_accepted_license":"1","scopus_import":"1","pmid":1,"external_id":{"isi":["000594648000014"],"pmid":["33188196"]},"file_date_updated":"2020-11-23T13:29:49Z","author":[{"full_name":"Nicolai, Leo","first_name":"Leo","last_name":"Nicolai"},{"full_name":"Schiefelbein, Karin","first_name":"Karin","last_name":"Schiefelbein"},{"last_name":"Lipsky","first_name":"Silvia","full_name":"Lipsky, Silvia"},{"first_name":"Alexander","last_name":"Leunig","full_name":"Leunig, Alexander"},{"full_name":"Hoffknecht, Marie","last_name":"Hoffknecht","first_name":"Marie"},{"full_name":"Pekayvaz, Kami","last_name":"Pekayvaz","first_name":"Kami"},{"last_name":"Raude","first_name":"Ben","full_name":"Raude, Ben"},{"full_name":"Marx, Charlotte","first_name":"Charlotte","last_name":"Marx"},{"full_name":"Ehrlich, Andreas","last_name":"Ehrlich","first_name":"Andreas"},{"first_name":"Joachim","last_name":"Pircher","full_name":"Pircher, Joachim"},{"first_name":"Zhe","last_name":"Zhang","full_name":"Zhang, Zhe"},{"full_name":"Saleh, Inas","first_name":"Inas","last_name":"Saleh"},{"first_name":"Anna-Kristina","last_name":"Marel","full_name":"Marel, Anna-Kristina"},{"full_name":"Löf, Achim","first_name":"Achim","last_name":"Löf"},{"full_name":"Petzold, Tobias","last_name":"Petzold","first_name":"Tobias"},{"full_name":"Lorenz, Michael","first_name":"Michael","last_name":"Lorenz"},{"full_name":"Stark, Konstantin","last_name":"Stark","first_name":"Konstantin"},{"first_name":"Robert","last_name":"Pick","full_name":"Pick, Robert"},{"last_name":"Rosenberger","first_name":"Gerhild","full_name":"Rosenberger, Gerhild"},{"full_name":"Weckbach, Ludwig","first_name":"Ludwig","last_name":"Weckbach"},{"last_name":"Uhl","first_name":"Bernd","full_name":"Uhl, Bernd"},{"full_name":"Xia, Sheng","first_name":"Sheng","last_name":"Xia"},{"last_name":"Reichel","first_name":"Christoph Andreas","full_name":"Reichel, Christoph Andreas"},{"first_name":"Barbara","last_name":"Walzog","full_name":"Walzog, Barbara"},{"first_name":"Christian","last_name":"Schulz","full_name":"Schulz, Christian"},{"first_name":"Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","last_name":"Zheden","full_name":"Zheden, Vanessa","orcid":"0000-0002-9438-4783"},{"full_name":"Bender, Markus","first_name":"Markus","last_name":"Bender"},{"first_name":"Rong","last_name":"Li","full_name":"Li, Rong"},{"full_name":"Massberg, Steffen","last_name":"Massberg","first_name":"Steffen"},{"last_name":"Gärtner","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","orcid":"0000-0001-6120-3723","full_name":"Gärtner, Florian R"}],"intvolume":" 11","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["20411723"]},"publication":"Nature Communications","acknowledgement":"We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M. [B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P. [Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]), FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.), FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.), and LMUexcellence NFF (F.G.). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie grant agreement no.\r\n747687.","doi":"10.1038/s41467-020-19515-0","publication_status":"published","volume":11,"type":"journal_article","status":"public","citation":{"chicago":"Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-19515-0.","mla":"Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” Nature Communications, vol. 11, 5778, Springer Nature, 2020, doi:10.1038/s41467-020-19515-0.","ista":"Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T, Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA, Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 11, 5778.","ama":"Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 2020;11. doi:10.1038/s41467-020-19515-0","apa":"Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz, K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-19515-0","short":"L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz, B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A. Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B. Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li, S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).","ieee":"L. Nicolai et al., “Vascular surveillance by haptotactic blood platelets in inflammation and infection,” Nature Communications, vol. 11. Springer Nature, 2020."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_number":"5778","date_published":"2020-11-13T00:00:00Z","month":"11","isi":1,"department":[{"_id":"MiSi"},{"_id":"EM-Fac"}],"_id":"8787","related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-31310-7","relation":"erratum"}]},"day":"13","date_updated":"2023-08-22T13:26:26Z","year":"2020"},{"publication_status":"published","status":"public","volume":11,"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"chicago":"Fäßler, Florian, Georgi A Dimchev, Victor-Valentin Hodirnau, William Wan, and Florian KM Schur. “Cryo-Electron Tomography Structure of Arp2/3 Complex in Cells Reveals New Insights into the Branch Junction.” Nature Communications. Springer Nature, 2020. https://doi.org/10.1038/s41467-020-20286-x.","mla":"Fäßler, Florian, et al. “Cryo-Electron Tomography Structure of Arp2/3 Complex in Cells Reveals New Insights into the Branch Junction.” Nature Communications, vol. 11, 6437, Springer Nature, 2020, doi:10.1038/s41467-020-20286-x.","ama":"Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature Communications. 2020;11. doi:10.1038/s41467-020-20286-x","ista":"Fäßler F, Dimchev GA, Hodirnau V-V, Wan W, Schur FK. 2020. Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature Communications. 11, 6437.","short":"F. Fäßler, G.A. Dimchev, V.-V. Hodirnau, W. Wan, F.K. Schur, Nature Communications 11 (2020).","apa":"Fäßler, F., Dimchev, G. A., Hodirnau, V.-V., Wan, W., & Schur, F. K. (2020). Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-020-20286-x","ieee":"F. Fäßler, G. A. Dimchev, V.-V. Hodirnau, W. Wan, and F. K. Schur, “Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction,” Nature Communications, vol. 11. Springer Nature, 2020."},"author":[{"full_name":"Fäßler, Florian","orcid":"0000-0001-7149-769X","id":"404F5528-F248-11E8-B48F-1D18A9856A87","first_name":"Florian","last_name":"Fäßler"},{"first_name":"Georgi A","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","last_name":"Dimchev","full_name":"Dimchev, Georgi A","orcid":"0000-0001-8370-6161"},{"last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87","first_name":"Victor-Valentin","full_name":"Hodirnau, Victor-Valentin"},{"first_name":"William","last_name":"Wan","full_name":"Wan, William"},{"last_name":"Schur","first_name":"Florian KM","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078","full_name":"Schur, Florian KM"}],"file_date_updated":"2020-12-28T08:16:10Z","keyword":["General Biochemistry","Genetics and Molecular Biology","General Physics and Astronomy","General Chemistry"],"publication_identifier":{"issn":["2041-1723"]},"intvolume":" 11","language":[{"iso":"eng"}],"doi":"10.1038/s41467-020-20286-x","acknowledgement":"This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the BioImaging Facility (BIF), and the Electron Microscopy Facility (EMF). We also thank Dimitry Tegunov (MPI for Biophysical Chemistry) for helpful discussions\r\nabout the M software, and Michael Sixt (IST Austria) and Klemens Rottner (Technical University Braunschweig, HZI Braunschweig) for critical reading of the manuscript. We also thank Gregory Voth (University of Chicago) for providing us the MD-derived branch junction model for comparison. The authors acknowledge support from IST Austria and from the Austrian Science Fund (FWF): M02495 to G.D. and Austrian Science Fund (FWF): P33367 to F.K.M.S. ","publication":"Nature Communications","related_material":{"link":[{"url":"https://ist.ac.at/en/news/cutting-edge-technology-reveals-structures-within-cells/","description":"News on IST Homepage","relation":"press_release"}]},"day":"22","date_updated":"2023-08-24T11:01:50Z","year":"2020","article_number":"6437","month":"12","date_published":"2020-12-22T00:00:00Z","_id":"8971","isi":1,"department":[{"_id":"FlSc"},{"_id":"EM-Fac"}],"oa_version":"Published Version","quality_controlled":"1","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"publisher":"Springer Nature","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Cryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction","date_created":"2020-12-23T08:25:45Z","oa":1,"article_type":"original","ddc":["570"],"scopus_import":"1","external_id":{"isi":["000603078000003"]},"project":[{"grant_number":"P33367","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A","name":"Structure and isoform diversity of the Arp2/3 complex"},{"name":"Protein structure and function in filopodia across scales","call_identifier":"FWF","grant_number":"M02495","_id":"2674F658-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","has_accepted_license":"1","file":[{"checksum":"55d43ea0061cc4027ba45e966e1db8cc","creator":"dernst","date_updated":"2020-12-28T08:16:10Z","relation":"main_file","access_level":"open_access","file_size":3958727,"success":1,"content_type":"application/pdf","file_id":"8975","date_created":"2020-12-28T08:16:10Z","file_name":"2020_NatureComm_Faessler.pdf"}],"abstract":[{"text":"The actin-related protein (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosis and pathogen infection. Its activation is tightly regulated and involves complex structural rearrangements and actin filament binding, which are yet to be understood. Here, we report a 9.0 Å resolution structure of the actin filament Arp2/3 complex branch junction in cells using cryo-electron tomography and subtomogram averaging. This allows us to generate an accurate model of the active Arp2/3 complex in the branch junction and its interaction with actin filaments. Notably, our model reveals a previously undescribed set of interactions of the Arp2/3 complex with the mother filament, significantly different to the previous branch junction model. Our structure also indicates a central role for the ArpC3 subunit in stabilizing the active conformation.","lang":"eng"}]},{"day":"01","date_updated":"2023-09-05T12:05:58Z","year":"2020","month":"07","date_published":"2020-07-01T00:00:00Z","_id":"10866","department":[{"_id":"NanoFab"}],"isi":1,"publication_status":"published","status":"public","type":"journal_article","volume":20,"citation":{"ama":"Duan J, Capote-Robayna N, Taboada-Gutiérrez J, et al. Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano Letters. 2020;20(7):5323-5329. doi:10.1021/acs.nanolett.0c01673","ista":"Duan J, Capote-Robayna N, Taboada-Gutiérrez J, Álvarez-Pérez G, Prieto Gonzalez I, Martín-Sánchez J, Nikitin AY, Alonso-González P. 2020. Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano Letters. 20(7), 5323–5329.","chicago":"Duan, Jiahua, Nathaniel Capote-Robayna, Javier Taboada-Gutiérrez, Gonzalo Álvarez-Pérez, Ivan Prieto Gonzalez, Javier Martín-Sánchez, Alexey Y. Nikitin, and Pablo Alonso-González. “Twisted Nano-Optics: Manipulating Light at the Nanoscale with Twisted Phonon Polaritonic Slabs.” Nano Letters. American Chemical Society, 2020. https://doi.org/10.1021/acs.nanolett.0c01673.","mla":"Duan, Jiahua, et al. “Twisted Nano-Optics: Manipulating Light at the Nanoscale with Twisted Phonon Polaritonic Slabs.” Nano Letters, vol. 20, no. 7, American Chemical Society, 2020, pp. 5323–29, doi:10.1021/acs.nanolett.0c01673.","ieee":"J. Duan et al., “Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs,” Nano Letters, vol. 20, no. 7. American Chemical Society, pp. 5323–5329, 2020.","apa":"Duan, J., Capote-Robayna, N., Taboada-Gutiérrez, J., Álvarez-Pérez, G., Prieto Gonzalez, I., Martín-Sánchez, J., … Alonso-González, P. (2020). Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs. Nano Letters. American Chemical Society. https://doi.org/10.1021/acs.nanolett.0c01673","short":"J. Duan, N. Capote-Robayna, J. Taboada-Gutiérrez, G. Álvarez-Pérez, I. Prieto Gonzalez, J. Martín-Sánchez, A.Y. Nikitin, P. Alonso-González, Nano Letters 20 (2020) 5323–5329."},"author":[{"full_name":"Duan, Jiahua","last_name":"Duan","first_name":"Jiahua"},{"full_name":"Capote-Robayna, Nathaniel","first_name":"Nathaniel","last_name":"Capote-Robayna"},{"last_name":"Taboada-Gutiérrez","first_name":"Javier","full_name":"Taboada-Gutiérrez, Javier"},{"full_name":"Álvarez-Pérez, Gonzalo","last_name":"Álvarez-Pérez","first_name":"Gonzalo"},{"last_name":"Prieto Gonzalez","id":"2A307FE2-F248-11E8-B48F-1D18A9856A87","first_name":"Ivan","orcid":"0000-0002-7370-5357","full_name":"Prieto Gonzalez, Ivan"},{"first_name":"Javier","last_name":"Martín-Sánchez","full_name":"Martín-Sánchez, Javier"},{"first_name":"Alexey Y.","last_name":"Nikitin","full_name":"Nikitin, Alexey Y."},{"full_name":"Alonso-González, Pablo","first_name":"Pablo","last_name":"Alonso-González"}],"keyword":["Mechanical Engineering","Condensed Matter Physics","General Materials Science","General Chemistry","Bioengineering"],"publication_identifier":{"eissn":["1530-6992"],"issn":["1530-6984"]},"language":[{"iso":"eng"}],"intvolume":" 20","doi":"10.1021/acs.nanolett.0c01673","acknowledgement":"J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa Program from the\r\nGovernment of the Principality of Asturias (nos. PA-18-PF-BP17-126 and PA20-PF-BP19-053,\r\nrespectively). J. M-S acknowledges financial support through the Ramón y Cajal Program from\r\nthe Government of Spain (RYC2018-026196-I). A.Y.N. acknowledges the Spanish Ministry of\r\nScience, Innovation and Universities (national project no. MAT201788358-C3-3-R). P.A.-G.\r\nacknowledges support from the European Research Council under starting grant no. 715496,\r\n2DNANOPTICA.","publication":"Nano Letters","scopus_import":"1","external_id":{"pmid":["32530634"],"isi":["000548893200082"],"arxiv":["2004.14599"]},"pmid":1,"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Recent discoveries have shown that, when two layers of van der Waals (vdW) materials are superimposed with a relative twist angle between them, the electronic properties of the coupled system can be dramatically altered. Here, we demonstrate that a similar concept can be extended to the optics realm, particularly to propagating phonon polaritons–hybrid light-matter interactions. To do this, we fabricate stacks composed of two twisted slabs of a vdW crystal (α-MoO3) supporting anisotropic phonon polaritons (PhPs), and image the propagation of the latter when launched by localized sources. Our images reveal that, under a critical angle, the PhPs isofrequency curve undergoes a topological transition, in which the propagation of PhPs is strongly guided (canalization regime) along predetermined directions without geometric spreading. These results demonstrate a new degree of freedom (twist angle) for controlling the propagation of polaritons at the nanoscale with potential for nanoimaging, (bio)-sensing, or heat management."}],"page":"5323-5329","oa_version":"Preprint","quality_controlled":"1","publisher":"American Chemical Society","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Twisted nano-optics: Manipulating light at the nanoscale with twisted phonon polaritonic slabs","main_file_link":[{"url":"https://arxiv.org/abs/2004.14599","open_access":"1"}],"issue":"7","date_created":"2022-03-18T11:37:38Z","oa":1,"article_type":"original"},{"citation":{"chicago":"Ernst, Doris, Gertraud Novotny, and Eva Maria Schönher. “(Core Trust) Seal your repository!” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, 2020. https://doi.org/10.31263/voebm.v73i1.3491.","mla":"Ernst, Doris, et al. “(Core Trust) Seal your repository!” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol. 73, no. 1, Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, 2020, pp. 46–59, doi:10.31263/voebm.v73i1.3491.","ama":"Ernst D, Novotny G, Schönher EM. (Core Trust) Seal your repository! Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 2020;73(1):46-59. doi:10.31263/voebm.v73i1.3491","ista":"Ernst D, Novotny G, Schönher EM. 2020. (Core Trust) Seal your repository! Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 73(1), 46–59.","apa":"Ernst, D., Novotny, G., & Schönher, E. M. (2020). (Core Trust) Seal your repository! Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare. https://doi.org/10.31263/voebm.v73i1.3491","short":"D. Ernst, G. Novotny, E.M. Schönher, Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare 73 (2020) 46–59.","ieee":"D. Ernst, G. Novotny, and E. M. Schönher, “(Core Trust) Seal your repository!,” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol. 73, no. 1. Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare, pp. 46–59, 2020."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"volume":73,"type":"journal_article","status":"public","publication_status":"published","publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","doi":"10.31263/voebm.v73i1.3491","language":[{"iso":"ger"}],"intvolume":" 73","publication_identifier":{"issn":["1022-2588"]},"file_date_updated":"2024-03-12T10:12:33Z","author":[{"last_name":"Ernst","id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","first_name":"Doris","orcid":"0000-0002-2354-0195","full_name":"Ernst, Doris"},{"last_name":"Novotny","first_name":"Gertraud","full_name":"Novotny, Gertraud"},{"first_name":"Eva Maria","last_name":"Schönher","full_name":"Schönher, Eva Maria"}],"year":"2020","date_updated":"2024-03-12T10:12:33Z","day":"28","department":[{"_id":"E-Lib"}],"_id":"7687","date_published":"2020-04-28T00:00:00Z","month":"04","issue":"1","popular_science":"1","title":"(Core Trust) Seal your repository!","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Vereinigung Osterreichischer Bibliothekarinnen und Bibliothekare","oa_version":"Published Version","ddc":["020"],"article_type":"original","oa":1,"date_created":"2020-04-28T08:37:38Z","scopus_import":"1","page":"46-59","has_accepted_license":"1","file":[{"file_id":"7970","content_type":"application/pdf","file_name":"2020_VOEB_Ernst.pdf","date_created":"2020-06-17T10:50:13Z","checksum":"fee784f15a489deb7def6ccf8c5bf8c3","creator":"dernst","date_updated":"2024-03-12T10:12:33Z","relation":"main_file","access_level":"open_access","file_size":579291}],"abstract":[{"text":"A working group, which was established within the Network of Repository Managers (RepManNet), has dealt with common certifications for repositories. In addition, current requirements of the research funding agencies FWF and EU were also taken into account. The Core Trust Seal was examined in more detail. For this purpose, a questionnaire was sent to those organizations that are already certified with CTS in Austria. The answers were summarized and evaluated anonymously. It is recommended to go for a repository certification. Moreover, the development of a DINI certificate in Austria is strongly suggested.","lang":"eng"},{"text":" Eine Arbeitsgruppe, die im Rahmen des Netzwerks für RepositorienmanagerInnen (RepManNet) entstanden ist, hat sich mit gängigen Zertifizierungen für Repositorien beschäftigt. Weiters wurden aktuelle Vorgaben der Forschungsförderer FWF und EU herangezogen. Das Core Trust Seal wurde genauer betrachtet. Hierfür wurden jenen Organisationen, die in Österreich bereits mit CTS zertifiziert sind, ein Fragebogen übermittelt. Die Antworten wurden anonymisiert zusammengefasst und ausgewertet. Plädiert wird für eine Zertifizierung von Repositorien und die Entwicklung einer DINI-Zertifizierung in Österreich.","lang":"ger"}],"article_processing_charge":"No"},{"date_created":"2020-05-05T14:31:33Z","oa":1,"ddc":["570"],"oa_version":"Preprint","acknowledged_ssus":[{"_id":"PreCl"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Cold Spring Harbor Laboratory","title":"Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development","project":[{"grant_number":"I03600","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules"},{"_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","name":"Molecular Drug Targets","call_identifier":"FWF"}],"article_processing_charge":"No","file":[{"date_created":"2020-05-05T14:31:19Z","file_name":"2020.01.10.902064v1.full.pdf","file_id":"7801","content_type":"application/pdf","file_size":2931370,"access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:48:03Z","creator":"rsix","checksum":"c6799ab5daba80efe8e2ed63c15f8c81"}],"abstract":[{"lang":"eng","text":"De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 (CUL3) lead to autism spectrum disorder (ASD). Here, we used Cul3 mouse models to evaluate the consequences of Cul3 mutations in vivo. Our results show that Cul3 haploinsufficient mice exhibit deficits in motor coordination as well as ASD-relevant social and cognitive impairments. Cul3 mutant brain displays cortical lamination abnormalities due to defective neuronal migration and reduced numbers of excitatory and inhibitory neurons. In line with the observed abnormal columnar organization, Cul3 haploinsufficiency is associated with decreased spontaneous excitatory and inhibitory activity in the cortex. At the molecular level, employing a quantitative proteomic approach, we show that Cul3 regulates cytoskeletal and adhesion protein abundance in mouse embryos. Abnormal regulation of cytoskeletal proteins in Cul3 mutant neuronal cells results in atypical organization of the actin mesh at the cell leading edge, likely causing the observed migration deficits. In contrast to these important functions early in development, Cul3 deficiency appears less relevant at adult stages. In fact, induction of Cul3 haploinsufficiency in adult mice does not result in the behavioral defects observed in constitutive Cul3 haploinsufficient animals. Taken together, our data indicate that Cul3 has a critical role in the regulation of cytoskeletal proteins and neuronal migration and that ASD-associated defects and behavioral abnormalities are primarily due to Cul3 functions at early developmental stages."}],"has_accepted_license":"1","author":[{"full_name":"Morandell, Jasmin","last_name":"Morandell","first_name":"Jasmin","id":"4739D480-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schwarz, Lena A","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","first_name":"Lena A","last_name":"Schwarz"},{"last_name":"Basilico","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","first_name":"Bernadette","orcid":"0000-0003-1843-3173","full_name":"Basilico, Bernadette"},{"last_name":"Tasciyan","first_name":"Saren","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren"},{"full_name":"Nicolas, Armel","last_name":"Nicolas","id":"2A103192-F248-11E8-B48F-1D18A9856A87","first_name":"Armel"},{"orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M"},{"full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87","first_name":"Caroline","last_name":"Kreuzinger"},{"id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87","first_name":"Lisa","last_name":"Knaus","full_name":"Knaus, Lisa"},{"first_name":"Zoe","id":"D23090A2-9057-11EA-883A-A8396FC7A38F","last_name":"Dobler","full_name":"Dobler, Zoe"},{"full_name":"Cacci, Emanuele","first_name":"Emanuele","last_name":"Cacci"},{"full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","first_name":"Johann G","last_name":"Danzl"},{"orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia","last_name":"Novarino","first_name":"Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2020-07-14T12:48:03Z","language":[{"iso":"eng"}],"doi":"10.1101/2020.01.10.902064 ","publication":"bioRxiv","publication_status":"submitted","status":"public","type":"preprint","citation":{"ama":"Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. bioRxiv. doi:10.1101/2020.01.10.902064 ","ista":"Morandell J, Schwarz LA, Basilico B, Tasciyan S, Nicolas A, Sommer CM, Kreuzinger C, Knaus L, Dobler Z, Cacci E, Danzl JG, Novarino G. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. bioRxiv, 10.1101/2020.01.10.902064 .","mla":"Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” BioRxiv, Cold Spring Harbor Laboratory, doi:10.1101/2020.01.10.902064 .","chicago":"Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan, Armel Nicolas, Christoph M Sommer, Caroline Kreuzinger, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” BioRxiv. Cold Spring Harbor Laboratory, n.d. https://doi.org/10.1101/2020.01.10.902064 .","ieee":"J. Morandell et al., “Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development,” bioRxiv. Cold Spring Harbor Laboratory.","apa":"Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Nicolas, A., Sommer, C. M., … Novarino, G. (n.d.). Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.01.10.902064 ","short":"J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, A. Nicolas, C.M. Sommer, C. Kreuzinger, L. Knaus, Z. Dobler, E. Cacci, J.G. Danzl, G. Novarino, BioRxiv (n.d.)."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"month":"01","date_published":"2020-01-11T00:00:00Z","_id":"7800","department":[{"_id":"JoDa"},{"_id":"GaNo"},{"_id":"LifeSc"}],"related_material":{"record":[{"status":"public","id":"9429","relation":"later_version"},{"relation":"dissertation_contains","id":"8620","status":"public"}]},"day":"11","date_updated":"2024-03-18T23:30:14Z","year":"2020"},{"page":"41","abstract":[{"text":"Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow1,2. Here we show in zebrafish primary germ layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase, and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. Once tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tension stabilizing E-cadherin-actin complexes at the contact.","lang":"eng"}],"article_processing_charge":"No","department":[{"_id":"CaHe"},{"_id":"EM-Fac"},{"_id":"Bio"}],"project":[{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"},{"_id":"2521E28E-B435-11E9-9278-68D0E5697425","grant_number":"187-2013","name":"Modulation of adhesion function in cell-cell contact formation by cortical tension"}],"_id":"9750","date_published":"2020-11-20T00:00:00Z","month":"11","year":"2020","date_updated":"2024-03-18T23:30:19Z","day":"20","related_material":{"record":[{"id":"10766","relation":"later_version","status":"public"},{"status":"public","id":"9623","relation":"dissertation_contains"}]},"publication":"bioRxiv","doi":"10.1101/2020.11.20.391284","acknowledgement":"We would like to thank Edouard Hannezo for discussions, Shayan Shami Pour and Daniel Capek for help with data analysis, Vanessa Barone and other members of the Heisenberg laboratory for thoughtful discussions and comments on the manuscript. We also thank Jack Merrin for preparing the microwells, and the Scientific Service Units at IST Austria, specifically Bioimaging and Electron Microscopy, and the Zebrafish Facility for continuous support. We acknowledge Hitoshi Morita for the kind gift of VinculinB-GFP plasmid. This research was supported by an ERC Advanced Grant (MECSPEC) to C.-P.H, EMBO Long Term grant (ALTF 187-2013) to M.S and IST Fellow Marie-Curie COFUND No. P_IST_EU01 to J.S.","language":[{"iso":"eng"}],"oa":1,"date_created":"2021-07-29T11:29:50Z","ec_funded":1,"author":[{"full_name":"Slovakova, Jana","last_name":"Slovakova","first_name":"Jana","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","first_name":"Mateusz K","last_name":"Sikora"},{"orcid":"0000-0002-5223-3346","full_name":"Caballero Mancebo, Silvia","last_name":"Caballero Mancebo","first_name":"Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel","last_name":"Krens","first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter"},{"id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","first_name":"Karla","last_name":"Huljev","full_name":"Huljev, Karla"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J"}],"main_file_link":[{"url":"https://doi.org/10.1101/2020.11.20.391284","open_access":"1"}],"citation":{"mla":"Slovakova, Jana, et al. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv, Cold Spring Harbor Laboratory, 2020, doi:10.1101/2020.11.20.391284.","chicago":"Slovakova, Jana, Mateusz K Sikora, Silvia Caballero Mancebo, Gabriel Krens, Walter Kaufmann, Karla Huljev, and Carl-Philipp J Heisenberg. “Tension-Dependent Stabilization of E-Cadherin Limits Cell-Cell Contact Expansion.” BioRxiv. Cold Spring Harbor Laboratory, 2020. https://doi.org/10.1101/2020.11.20.391284.","ama":"Slovakova J, Sikora MK, Caballero Mancebo S, et al. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv. 2020. doi:10.1101/2020.11.20.391284","ista":"Slovakova J, Sikora MK, Caballero Mancebo S, Krens G, Kaufmann W, Huljev K, Heisenberg C-PJ. 2020. Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv, 10.1101/2020.11.20.391284.","apa":"Slovakova, J., Sikora, M. K., Caballero Mancebo, S., Krens, G., Kaufmann, W., Huljev, K., & Heisenberg, C.-P. J. (2020). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion. bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.11.20.391284","short":"J. Slovakova, M.K. Sikora, S. Caballero Mancebo, G. Krens, W. Kaufmann, K. Huljev, C.-P.J. Heisenberg, BioRxiv (2020).","ieee":"J. Slovakova et al., “Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion,” bioRxiv. Cold Spring Harbor Laboratory, 2020."},"title":"Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion","type":"preprint","publisher":"Cold Spring Harbor Laboratory","status":"public","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"SSU"}],"oa_version":"Preprint","publication_status":"published"},{"external_id":{"isi":["000532688300008"]},"scopus_import":"1","abstract":[{"text":"Eukaryotic cells migrate by coupling the intracellular force of the actin cytoskeleton to the environment. While force coupling is usually mediated by transmembrane adhesion receptors, especially those of the integrin family, amoeboid cells such as leukocytes can migrate extremely fast despite very low adhesive forces1. Here we show that leukocytes cannot only migrate under low adhesion but can also transmit forces in the complete absence of transmembrane force coupling. When confined within three-dimensional environments, they use the topographical features of the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton follows the texture of the substrate, creating retrograde shear forces that are sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent migration are not mutually exclusive, but rather are variants of the same principle of coupling retrograde actin flow to the environment and thus can potentially operate interchangeably and simultaneously. As adhesion-free migration is independent of the chemical composition of the environment, it renders cells completely autonomous in their locomotive behaviour.","lang":"eng"}],"page":"582–585","project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","call_identifier":"FP7"},{"grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Cellular navigation along spatial gradients"},{"name":"Mechanical adaptation of lamellipodial actin","call_identifier":"FWF","grant_number":"P29911","_id":"26018E70-B435-11E9-9278-68D0E5697425"},{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687"}],"article_processing_charge":"No","publisher":"Springer Nature","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Cellular locomotion using environmental topography","oa_version":"None","quality_controlled":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"article_type":"original","ec_funded":1,"date_created":"2020-05-24T22:01:01Z","year":"2020","date_updated":"2024-03-18T23:30:24Z","day":"25","related_material":{"link":[{"url":"https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/","relation":"press_release","description":"News on IST Homepage"}],"record":[{"relation":"dissertation_contains","id":"14697","status":"public"},{"status":"public","relation":"dissertation_contains","id":"12401"}]},"_id":"7885","isi":1,"department":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"MiSi"}],"month":"06","date_published":"2020-06-25T00:00:00Z","citation":{"ista":"Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL, de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK. 2020. Cellular locomotion using environmental topography. Nature. 582, 582–585.","ama":"Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental topography. Nature. 2020;582:582–585. doi:10.1038/s41586-020-2283-z","mla":"Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.” Nature, vol. 582, Springer Nature, 2020, pp. 582–585, doi:10.1038/s41586-020-2283-z.","chicago":"Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan, Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental Topography.” Nature. Springer Nature, 2020. https://doi.org/10.1038/s41586-020-2283-z.","ieee":"A. Reversat et al., “Cellular locomotion using environmental topography,” Nature, vol. 582. Springer Nature, pp. 582–585, 2020.","apa":"Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography. Nature. Springer Nature. https://doi.org/10.1038/s41586-020-2283-z","short":"A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez, M.K. Sixt, Nature 582 (2020) 582–585."},"status":"public","type":"journal_article","volume":582,"publication_status":"published","acknowledgement":"We thank A. Leithner and J. Renkawitz for discussion and critical reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic setups; the Bioimaging Facility of IST Austria for excellent support, as well as the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan, L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687.","doi":"10.1038/s41586-020-2283-z","publication":"Nature","publication_identifier":{"eissn":["14764687"],"issn":["00280836"]},"language":[{"iso":"eng"}],"intvolume":" 582","author":[{"full_name":"Reversat, Anne","orcid":"0000-0003-0666-8928","id":"35B76592-F248-11E8-B48F-1D18A9856A87","first_name":"Anne","last_name":"Reversat"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","last_name":"Gärtner","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723"},{"first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"full_name":"Stopp, Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","first_name":"Julian A","last_name":"Stopp"},{"orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","last_name":"Tasciyan","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren"},{"last_name":"Aguilera Servin","first_name":"Juan L","id":"2A67C376-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2862-8372","full_name":"Aguilera Servin, Juan L"},{"full_name":"De Vries, Ingrid","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"id":"4167FE56-F248-11E8-B48F-1D18A9856A87","first_name":"Miroslav","last_name":"Hons","full_name":"Hons, Miroslav","orcid":"0000-0002-6625-3348"},{"last_name":"Piel","first_name":"Matthieu","full_name":"Piel, Matthieu"},{"full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones","first_name":"Andrew"},{"last_name":"Voituriez","first_name":"Raphael","full_name":"Voituriez, Raphael"},{"orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}]},{"article_number":"jcs248062","month":"08","date_published":"2020-08-06T00:00:00Z","_id":"8139","isi":1,"department":[{"_id":"JiFr"},{"_id":"EM-Fac"}],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"14510"}]},"day":"06","date_updated":"2023-12-01T13:51:07Z","year":"2020","author":[{"full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","first_name":"Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","last_name":"Johnson"},{"last_name":"Gnyliukh","first_name":"Nataliia","id":"390C1120-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2198-0509","full_name":"Gnyliukh, Nataliia"},{"full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann"},{"last_name":"Narasimhan","first_name":"Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8600-0671","full_name":"Narasimhan, Madhumitha"},{"last_name":"Vert","first_name":"G","full_name":"Vert, G"},{"full_name":"Bednarek, SY","last_name":"Bednarek","first_name":"SY"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"file_date_updated":"2021-08-08T22:30:03Z","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"language":[{"iso":"eng"}],"intvolume":" 133","acknowledgement":"This paper is dedicated to the memory of Christien Merrifield. He pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship inspired the development of all\r\nthe analysis methods presented here. His joy in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP plants used in this manuscript. We further thank the\r\nScientific Service Units at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility for access to equipment. ","doi":"10.1242/jcs.248062","publication":"Journal of Cell Science","publication_status":"published","status":"public","volume":133,"type":"journal_article","citation":{"ieee":"A. J. Johnson et al., “Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis,” Journal of Cell Science, vol. 133, no. 15. The Company of Biologists, 2020.","apa":"Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek, S., & Friml, J. (2020). Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell Science. The Company of Biologists. https://doi.org/10.1242/jcs.248062","short":"A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek, J. Friml, Journal of Cell Science 133 (2020).","ama":"Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell Science. 2020;133(15). doi:10.1242/jcs.248062","ista":"Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15), jcs248062.","mla":"Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of Cell Science, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020, doi:10.1242/jcs.248062.","chicago":"Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan, G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” Journal of Cell Science. The Company of Biologists, 2020. https://doi.org/10.1242/jcs.248062."},"project":[{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425"},{"grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program","call_identifier":"H2020"}],"article_processing_charge":"No","abstract":[{"text":"Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and inter-cellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how it functions in planta. In order to facilitate the direct quantitative study of plant CME, here we review current routinely used methods and present refined, standardized quantitative imaging protocols which allow the detailed characterization of CME at multiple scales in plant tissues. These include: (i) an efficient electron microscopy protocol for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for the detailed characterization of the ultra-structure of clathrin-coated vesicles; (ii) a detailed protocol and analysis for quantitative live-cell fluorescence microscopy to precisely examine the temporal interplay of endocytosis components during single CME events; (iii) a semi-automated analysis to allow the quantitative characterization of global internalization of cargos in whole plant tissues; and (iv) an overview and validation of useful genetic and pharmacological tools to interrogate the molecular mechanisms and function of CME in intact plant samples.","lang":"eng"}],"has_accepted_license":"1","file":[{"date_created":"2020-11-26T17:12:51Z","file_name":"2020 - Johnson - JSC - plant CME toolbox.pdf","file_id":"8815","content_type":"application/pdf","embargo":"2021-08-07","file_size":15150403,"relation":"main_file","access_level":"open_access","date_updated":"2021-08-08T22:30:03Z","checksum":"2d11f79a0b4e0a380fb002b933da331a","creator":"ajohnson"}],"scopus_import":"1","external_id":{"pmid":["32616560"],"isi":["000561047900021"]},"pmid":1,"ec_funded":1,"date_created":"2020-07-21T08:58:19Z","oa":1,"ddc":["575"],"article_type":"original","oa_version":"Published Version","quality_controlled":"1","acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"The Company of Biologists","title":"Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis","issue":"15"},{"quality_controlled":"1","oa_version":"Published Version","title":"Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publisher":"BioMed Central","ddc":["570"],"oa":1,"date_created":"2019-08-18T22:00:39Z","pmid":1,"external_id":{"pmid":["31395095"]},"scopus_import":1,"article_processing_charge":"No","has_accepted_license":"1","abstract":[{"text":"Glyphosate (N-phosphonomethyl glycine) and its commercial herbicide formulations have been shown to exert toxicity via various mechanisms. It has been asserted that glyphosate substitutes for glycine in polypeptide chains leading to protein misfolding and toxicity. However, as no direct evidence exists for glycine to glyphosate substitution in proteins, including in mammalian organisms, we tested this claim by conducting a proteomics analysis of MDA-MB-231 human breast cancer cells grown in the presence of 100 mg/L glyphosate for 6 days. Protein extracts from three treated and three untreated cell cultures were analysed as one TMT-6plex labelled sample, to highlight a specific pattern (+/+/+/−/−/−) of reporter intensities for peptides bearing true glyphosate treatment induced-post translational modifications as well as allowing an investigation of the total proteome.","lang":"eng"}],"file":[{"access_level":"open_access","relation":"main_file","file_size":1177482,"checksum":"4a2bb7994b7f2c432bf44f5127ea3102","creator":"dernst","date_updated":"2020-07-14T12:47:40Z","file_id":"6829","content_type":"application/pdf","file_name":"2019_BMC_Antoniou.pdf","date_created":"2019-08-23T11:10:35Z"}],"citation":{"ista":"Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. 2019. Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. BMC Research Notes. 12, 494.","ama":"Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. BMC Research Notes. 2019;12. doi:10.1186/s13104-019-4534-3","chicago":"Antoniou, Michael N., Armel Nicolas, Robin Mesnage, Martina Biserni, Francesco V. Rao, and Cristina Vazquez Martin. “Glyphosate Does Not Substitute for Glycine in Proteins of Actively Dividing Mammalian Cells.” BMC Research Notes. BioMed Central, 2019. https://doi.org/10.1186/s13104-019-4534-3.","mla":"Antoniou, Michael N., et al. “Glyphosate Does Not Substitute for Glycine in Proteins of Actively Dividing Mammalian Cells.” BMC Research Notes, vol. 12, 494, BioMed Central, 2019, doi:10.1186/s13104-019-4534-3.","ieee":"M. N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F. V. Rao, and C. V. Martin, “Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells,” BMC Research Notes, vol. 12. BioMed Central, 2019.","short":"M.N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F.V. Rao, C.V. Martin, BMC Research Notes 12 (2019).","apa":"Antoniou, M. N., Nicolas, A., Mesnage, R., Biserni, M., Rao, F. V., & Martin, C. V. (2019). Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. BMC Research Notes. BioMed Central. https://doi.org/10.1186/s13104-019-4534-3"},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"publication_status":"published","volume":12,"type":"journal_article","status":"public","intvolume":" 12","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1756-0500"]},"publication":"BMC Research Notes","doi":"10.1186/s13104-019-4534-3","file_date_updated":"2020-07-14T12:47:40Z","author":[{"full_name":"Antoniou, Michael N.","last_name":"Antoniou","first_name":"Michael N."},{"last_name":"Nicolas","first_name":"Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","full_name":"Nicolas, Armel"},{"first_name":"Robin","last_name":"Mesnage","full_name":"Mesnage, Robin"},{"full_name":"Biserni, Martina","last_name":"Biserni","first_name":"Martina"},{"last_name":"Rao","first_name":"Francesco V.","full_name":"Rao, Francesco V."},{"first_name":"Cristina Vazquez","last_name":"Martin","full_name":"Martin, Cristina Vazquez"}],"date_updated":"2023-02-23T14:08:14Z","year":"2019","related_material":{"record":[{"id":"9784","relation":"research_data","status":"public"}]},"day":"08","department":[{"_id":"LifeSc"}],"_id":"6819","article_number":"494","date_published":"2019-08-08T00:00:00Z","month":"08"},{"citation":{"chicago":"Antoniou, Michael N., Armel Nicolas, Robin Mesnage, Martina Biserni, Francesco V. Rao, and Cristina Vazquez Martin. “MOESM1 of Glyphosate Does Not Substitute for Glycine in Proteins of Actively Dividing Mammalian Cells.” Springer Nature, 2019. https://doi.org/10.6084/m9.figshare.9411761.v1.","mla":"Antoniou, Michael N., et al. MOESM1 of Glyphosate Does Not Substitute for Glycine in Proteins of Actively Dividing Mammalian Cells. Springer Nature, 2019, doi:10.6084/m9.figshare.9411761.v1.","ista":"Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. 2019. MOESM1 of Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells, Springer Nature, 10.6084/m9.figshare.9411761.v1.","ama":"Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. MOESM1 of Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. 2019. doi:10.6084/m9.figshare.9411761.v1","apa":"Antoniou, M. N., Nicolas, A., Mesnage, R., Biserni, M., Rao, F. V., & Martin, C. V. (2019). MOESM1 of Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells. Springer Nature. https://doi.org/10.6084/m9.figshare.9411761.v1","short":"M.N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F.V. Rao, C.V. Martin, (2019).","ieee":"M. N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F. V. Rao, and C. V. Martin, “MOESM1 of Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells.” Springer Nature, 2019."},"main_file_link":[{"url":"https://doi.org/10.6084/m9.figshare.9411761.v1","open_access":"1"}],"oa_version":"Published Version","type":"research_data_reference","title":"MOESM1 of Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells","status":"public","user_id":"6785fbc1-c503-11eb-8a32-93094b40e1cf","publisher":"Springer Nature","oa":1,"doi":"10.6084/m9.figshare.9411761.v1","author":[{"first_name":"Michael N.","last_name":"Antoniou","full_name":"Antoniou, Michael N."},{"full_name":"Nicolas, Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","first_name":"Armel","last_name":"Nicolas"},{"last_name":"Mesnage","first_name":"Robin","full_name":"Mesnage, Robin"},{"last_name":"Biserni","first_name":"Martina","full_name":"Biserni, Martina"},{"full_name":"Rao, Francesco V.","last_name":"Rao","first_name":"Francesco V."},{"last_name":"Martin","first_name":"Cristina Vazquez","full_name":"Martin, Cristina Vazquez"}],"date_created":"2021-08-06T08:14:05Z","date_updated":"2023-02-23T12:52:29Z","year":"2019","related_material":{"record":[{"id":"6819","relation":"used_in_publication","status":"public"}]},"day":"09","article_processing_charge":"No","department":[{"_id":"LifeSc"}],"_id":"9784","abstract":[{"text":"Additional file 1: Table S1. Kinetics of MDA-MB-231 cell growth in either the presence or absence of 100Â mg/L glyphosate. Cell counts are given at day-1 of seeding flasks and following 6-days of continuous culture. Note: no differences in cell numbers were observed between negative control and glyphosate treated cultures.","lang":"eng"}],"date_published":"2019-08-09T00:00:00Z","month":"08"},{"author":[{"first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"full_name":"Kiss, Janos","last_name":"Kiss","first_name":"Janos","id":"3D3A06F8-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Elefante","id":"490F40CE-F248-11E8-B48F-1D18A9856A87","first_name":"Stefano","full_name":"Elefante, Stefano"}],"file_date_updated":"2023-05-16T07:27:09Z","date_created":"2023-05-05T12:48:48Z","oa":1,"ddc":["000"],"language":[{"iso":"eng"}],"publication":"AHPC19 - Austrian HPC Meeting 2019 ","publication_status":"published","oa_version":"Published Version","conference":{"end_date":"2019-02-27","name":"AHPC: Austrian HPC Meeting","location":"Grundlsee, Austria","start_date":"2019-02-25"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz","status":"public","type":"conference_abstract","title":"Is Debian suitable for running an HPC Cluster?","citation":{"short":"A. Schlögl, J. Kiss, S. Elefante, in:, AHPC19 - Austrian HPC Meeting 2019 , Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz, 2019, p. 25.","apa":"Schlögl, A., Kiss, J., & Elefante, S. (2019). Is Debian suitable for running an HPC Cluster? In AHPC19 - Austrian HPC Meeting 2019 (p. 25). Grundlsee, Austria: Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz.","ieee":"A. Schlögl, J. Kiss, and S. Elefante, “Is Debian suitable for running an HPC Cluster?,” in AHPC19 - Austrian HPC Meeting 2019 , Grundlsee, Austria, 2019, p. 25.","chicago":"Schlögl, Alois, Janos Kiss, and Stefano Elefante. “Is Debian Suitable for Running an HPC Cluster?” In AHPC19 - Austrian HPC Meeting 2019 , 25. Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz, 2019.","mla":"Schlögl, Alois, et al. “Is Debian Suitable for Running an HPC Cluster?” AHPC19 - Austrian HPC Meeting 2019 , Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz, 2019, p. 25.","ista":"Schlögl A, Kiss J, Elefante S. 2019. Is Debian suitable for running an HPC Cluster? AHPC19 - Austrian HPC Meeting 2019 . AHPC: Austrian HPC Meeting, 25.","ama":"Schlögl A, Kiss J, Elefante S. Is Debian suitable for running an HPC Cluster? In: AHPC19 - Austrian HPC Meeting 2019 . Institut für Mathematik und wissenschaftliches Rechnen der Universität Graz; 2019:25."},"main_file_link":[{"open_access":"1","url":"https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc19/BOOKLET_AHPC19.pdf"}],"month":"02","date_published":"2019-02-27T00:00:00Z","_id":"12901","article_processing_charge":"No","department":[{"_id":"ScienComp"}],"has_accepted_license":"1","page":"25","file":[{"success":1,"content_type":"application/pdf","file_id":"12970","file_name":"2019_AHPC_Schloegl.pdf","date_created":"2023-05-16T07:27:09Z","date_updated":"2023-05-16T07:27:09Z","checksum":"acc8272027faaf30709c51ac5c58ffa4","creator":"dernst","file_size":1097603,"access_level":"open_access","relation":"main_file"}],"day":"27","date_updated":"2023-05-16T07:29:32Z","year":"2019"},{"publisher":"Nature Publishing Group","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"A practical guide to optimization in X10 expansion microscopy","quality_controlled":"1","oa_version":"Submitted Version","issue":"3","ec_funded":1,"date_created":"2019-02-24T22:59:20Z","oa":1,"ddc":["570"],"article_type":"original","scopus_import":"1","external_id":{"isi":["000459890700008"],"pmid":["30778205"]},"pmid":1,"abstract":[{"lang":"eng","text":"Expansion microscopy is a relatively new approach to super-resolution imaging that uses expandable hydrogels to isotropically increase the physical distance between fluorophores in biological samples such as cell cultures or tissue slices. The classic gel recipe results in an expansion factor of ~4×, with a resolution of 60–80 nm. We have recently developed X10 microscopy, which uses a gel that achieves an expansion factor of ~10×, with a resolution of ~25 nm. Here, we provide a step-by-step protocol for X10 expansion microscopy. A typical experiment consists of seven sequential stages: (i) immunostaining, (ii) anchoring, (iii) polymerization, (iv) homogenization, (v) expansion, (vi) imaging, and (vii) validation. The protocol presented here includes recommendations for optimization, pitfalls and their solutions, and detailed guidelines that should increase reproducibility. Although our protocol focuses on X10 expansion microscopy, we detail which of these suggestions are also applicable to classic fourfold expansion microscopy. We exemplify our protocol using primary hippocampal neurons from rats, but our approach can be used with other primary cells or cultured cell lines of interest. This protocol will enable any researcher with basic experience in immunostainings and access to an epifluorescence microscope to perform super-resolution microscopy with X10. The procedure takes 3 d and requires ~5 h of actively handling the sample for labeling and expansion, and another ~3 h for imaging and analysis."}],"page":"832–863","file":[{"date_updated":"2021-06-29T14:41:46Z","creator":"kschuh","checksum":"7efb9951e7ddf3e3dcc2fb92b859c623","file_size":84478958,"access_level":"open_access","relation":"main_file","success":1,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_id":"9619","date_created":"2021-06-29T14:41:46Z","file_name":"181031_Truckenbrodt_ExM_NatProtoc.docx"}],"has_accepted_license":"1","project":[{"name":"ISTplus - Postdoctoral Fellowships","call_identifier":"H2020","_id":"260C2330-B435-11E9-9278-68D0E5697425","grant_number":"754411"},{"name":"Optical control of synaptic function via adhesion molecules","call_identifier":"FWF","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","grant_number":"I03600"}],"article_processing_charge":"No","status":"public","type":"journal_article","volume":14,"publication_status":"published","citation":{"ieee":"S. M. Truckenbrodt, C. M. Sommer, S. O. Rizzoli, and J. G. Danzl, “A practical guide to optimization in X10 expansion microscopy,” Nature Protocols, vol. 14, no. 3. Nature Publishing Group, pp. 832–863, 2019.","apa":"Truckenbrodt, S. M., Sommer, C. M., Rizzoli, S. O., & Danzl, J. G. (2019). A practical guide to optimization in X10 expansion microscopy. Nature Protocols. Nature Publishing Group. https://doi.org/10.1038/s41596-018-0117-3","short":"S.M. Truckenbrodt, C.M. Sommer, S.O. Rizzoli, J.G. Danzl, Nature Protocols 14 (2019) 832–863.","ama":"Truckenbrodt SM, Sommer CM, Rizzoli SO, Danzl JG. A practical guide to optimization in X10 expansion microscopy. Nature Protocols. 2019;14(3):832–863. doi:10.1038/s41596-018-0117-3","ista":"Truckenbrodt SM, Sommer CM, Rizzoli SO, Danzl JG. 2019. A practical guide to optimization in X10 expansion microscopy. Nature Protocols. 14(3), 832–863.","mla":"Truckenbrodt, Sven M., et al. “A Practical Guide to Optimization in X10 Expansion Microscopy.” Nature Protocols, vol. 14, no. 3, Nature Publishing Group, 2019, pp. 832–863, doi:10.1038/s41596-018-0117-3.","chicago":"Truckenbrodt, Sven M, Christoph M Sommer, Silvio O Rizzoli, and Johann G Danzl. “A Practical Guide to Optimization in X10 Expansion Microscopy.” Nature Protocols. Nature Publishing Group, 2019. https://doi.org/10.1038/s41596-018-0117-3."},"author":[{"full_name":"Truckenbrodt, Sven M","last_name":"Truckenbrodt","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","first_name":"Sven M"},{"orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M"},{"first_name":"Silvio O","last_name":"Rizzoli","full_name":"Rizzoli, Silvio O"},{"first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973"}],"file_date_updated":"2021-06-29T14:41:46Z","doi":"10.1038/s41596-018-0117-3","publication":"Nature Protocols","language":[{"iso":"eng"}],"intvolume":" 14","day":"01","year":"2019","date_updated":"2023-08-24T14:48:33Z","month":"03","date_published":"2019-03-01T00:00:00Z","_id":"6052","department":[{"_id":"JoDa"},{"_id":"Bio"}],"isi":1},{"citation":{"mla":"Xia, Peng, et al. “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.” Cell, vol. 176, no. 6, Elsevier, 2019, p. 1379–1392.e14, doi:10.1016/j.cell.2019.01.019.","chicago":"Xia, Peng, Daniel J Gütl, Vanessa Zheden, and Carl-Philipp J Heisenberg. “Lateral Inhibition in Cell Specification Mediated by Mechanical Signals Modulating TAZ Activity.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.01.019.","ista":"Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. 2019. Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. 176(6), 1379–1392.e14.","ama":"Xia P, Gütl DJ, Zheden V, Heisenberg C-PJ. Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. 2019;176(6):1379-1392.e14. doi:10.1016/j.cell.2019.01.019","short":"P. Xia, D.J. Gütl, V. Zheden, C.-P.J. Heisenberg, Cell 176 (2019) 1379–1392.e14.","apa":"Xia, P., Gütl, D. J., Zheden, V., & Heisenberg, C.-P. J. (2019). Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.01.019","ieee":"P. Xia, D. J. Gütl, V. Zheden, and C.-P. J. Heisenberg, “Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity,” Cell, vol. 176, no. 6. Elsevier, p. 1379–1392.e14, 2019."},"publication_status":"published","type":"journal_article","volume":176,"status":"public","intvolume":" 176","language":[{"iso":"eng"}],"publication":"Cell","doi":"10.1016/j.cell.2019.01.019","acknowledgement":"We thank Roland Dosch, Makoto Furutani-Seiki, Brian Link, Mary Mullins, and Masazumi Tada for providing transgenic and/or mutant zebrafish lines; Alexandra Schauer, Shayan Shami-Pour, and the rest of the Heisenberg lab for technical assistance and feedback on the manuscript; and the Bioimaging, Electron Microscopy, and Zebrafish facilities of IST Austria for continuous support. This work was supported by an ERC advanced grant ( MECSPEC to C.-P.H.).","author":[{"first_name":"Peng","id":"4AB6C7D0-F248-11E8-B48F-1D18A9856A87","last_name":"Xia","full_name":"Xia, Peng","orcid":"0000-0002-5419-7756"},{"last_name":"Gütl","first_name":"Daniel J","id":"381929CE-F248-11E8-B48F-1D18A9856A87","full_name":"Gütl, Daniel J"},{"orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa","last_name":"Zheden","first_name":"Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"date_updated":"2023-08-25T08:02:23Z","year":"2019","related_material":{"link":[{"url":"https://ist.ac.at/en/news/in-zebrafish-eggs-most-rapidly-growing-cell-inhibits-its-neighbours-through-mechanical-signals/","description":"News on IST Homepage","relation":"press_release"}]},"day":"07","isi":1,"department":[{"_id":"CaHe"},{"_id":"EM-Fac"}],"_id":"6087","date_published":"2019-03-07T00:00:00Z","month":"03","issue":"6","main_file_link":[{"url":"https://doi.org/10.1016/j.cell.2019.01.019","open_access":"1"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"oa_version":"Published Version","quality_controlled":"1","title":"Lateral inhibition in cell specification mediated by mechanical signals modulating TAZ activity","publisher":"Elsevier","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_type":"original","oa":1,"date_created":"2019-03-10T22:59:19Z","ec_funded":1,"pmid":1,"external_id":{"pmid":["30773315"],"isi":["000460509600013"]},"scopus_import":"1","article_processing_charge":"No","project":[{"grant_number":"742573","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation"}],"page":"1379-1392.e14","abstract":[{"lang":"eng","text":"Cell fate specification by lateral inhibition typically involves contact signaling through the Delta-Notch signaling pathway. However, whether this is the only signaling mode mediating lateral inhibition remains unclear. Here we show that in zebrafish oogenesis, a group of cells within the granulosa cell layer at the oocyte animal pole acquire elevated levels of the transcriptional coactivator TAZ in their nuclei. One of these cells, the future micropyle precursor cell (MPC), accumulates increasingly high levels of nuclear TAZ and grows faster than its surrounding cells, mechanically compressing those cells, which ultimately lose TAZ from their nuclei. Strikingly, relieving neighbor-cell compression by MPC ablation or aspiration restores nuclear TAZ accumulation in neighboring cells, eventually leading to MPC re-specification from these cells. Conversely, MPC specification is defective in taz−/− follicles. These findings uncover a novel mode of lateral inhibition in cell fate specification based on mechanical signals controlling TAZ activity."}]},{"external_id":{"isi":["000472597400042"]},"scopus_import":"1","article_processing_charge":"No","abstract":[{"text":"Acute myeloid leukemia (AML) is a heterogeneous disease with respect to its genetic and molecular basis and to patients´ outcome. Clinical, cytogenetic, and mutational data are used to classify patients into risk groups with different survival, however, within-group heterogeneity is still an issue. Here, we used a robust likelihood-based survival modeling approach and publicly available gene expression data to identify a minimal number of genes whose combined expression values were prognostic of overall survival. The resulting gene expression signature (4-GES) consisted of 4 genes (SOCS2, IL2RA, NPDC1, PHGDH), predicted patient survival as an independent prognostic parameter in several cohorts of AML patients (total, 1272 patients), and further refined prognostication based on the European Leukemia Net classification. An oncogenic role of the top scoring gene in this signature, SOCS2, was investigated using MLL-AF9 and Flt3-ITD/NPM1c driven mouse models of AML. SOCS2 promoted leukemogenesis as well as the abundance, quiescence, and activity of AML stem cells. Overall, the 4-GES represents a highly discriminating prognostic parameter in AML, whose clinical applicability is greatly enhanced by its small number of genes. The newly established role of SOCS2 in leukemia aggressiveness and stemness raises the possibility that the signature might even be exploitable therapeutically.","lang":"eng"}],"has_accepted_license":"1","file":[{"date_updated":"2020-07-14T12:47:34Z","checksum":"3283522fffadf4b5fc8c7adfe3ba4564","creator":"kschuh","file_size":2017352,"relation":"main_file","access_level":"open_access","file_name":"nature_2019_Nguyen.pdf","date_created":"2019-07-08T15:15:28Z","file_id":"6623","content_type":"application/pdf"}],"issue":"1","oa_version":"Published Version","quality_controlled":"1","publisher":"Nature Publishing Group","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness","oa":1,"ddc":["576"],"date_created":"2019-07-07T21:59:19Z","date_updated":"2023-08-28T12:26:51Z","year":"2019","day":"24","_id":"6607","isi":1,"department":[{"_id":"PreCl"}],"article_number":"9139","month":"06","date_published":"2019-06-24T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"C. H. Nguyen et al., “SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness,” Scientific Reports, vol. 9, no. 1. Nature Publishing Group, 2019.","apa":"Nguyen, C. H., Glüxam, T., Schlerka, A., Bauer, K., Grandits, A. M., Hackl, H., … Heller, G. (2019). SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness. Scientific Reports. Nature Publishing Group. https://doi.org/10.1038/s41598-019-45579-0","short":"C.H. Nguyen, T. Glüxam, A. Schlerka, K. Bauer, A.M. Grandits, H. Hackl, O. Dovey, S. Zöchbauer-Müller, J.L. Cooper, G.S. Vassiliou, D. Stoiber, R. Wieser, G. Heller, Scientific Reports 9 (2019).","ista":"Nguyen CH, Glüxam T, Schlerka A, Bauer K, Grandits AM, Hackl H, Dovey O, Zöchbauer-Müller S, Cooper JL, Vassiliou GS, Stoiber D, Wieser R, Heller G. 2019. SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness. Scientific Reports. 9(1), 9139.","ama":"Nguyen CH, Glüxam T, Schlerka A, et al. SOCS2 is part of a highly prognostic 4-gene signature in AML and promotes disease aggressiveness. Scientific Reports. 2019;9(1). doi:10.1038/s41598-019-45579-0","mla":"Nguyen, Chi Huu, et al. “SOCS2 Is Part of a Highly Prognostic 4-Gene Signature in AML and Promotes Disease Aggressiveness.” Scientific Reports, vol. 9, no. 1, 9139, Nature Publishing Group, 2019, doi:10.1038/s41598-019-45579-0.","chicago":"Nguyen, Chi Huu, Tobias Glüxam, Angela Schlerka, Katharina Bauer, Alexander M. Grandits, Hubert Hackl, Oliver Dovey, et al. “SOCS2 Is Part of a Highly Prognostic 4-Gene Signature in AML and Promotes Disease Aggressiveness.” Scientific Reports. Nature Publishing Group, 2019. https://doi.org/10.1038/s41598-019-45579-0."},"publication_status":"published","status":"public","type":"journal_article","volume":9,"intvolume":" 9","language":[{"iso":"eng"}],"doi":"10.1038/s41598-019-45579-0","publication":"Scientific Reports","author":[{"full_name":"Nguyen, Chi Huu","first_name":"Chi Huu","last_name":"Nguyen"},{"first_name":"Tobias","last_name":"Glüxam","full_name":"Glüxam, Tobias"},{"full_name":"Schlerka, Angela","last_name":"Schlerka","first_name":"Angela"},{"full_name":"Bauer, Katharina","last_name":"Bauer","id":"2ED6B14C-F248-11E8-B48F-1D18A9856A87","first_name":"Katharina"},{"full_name":"Grandits, Alexander M.","first_name":"Alexander M.","last_name":"Grandits"},{"full_name":"Hackl, Hubert","last_name":"Hackl","first_name":"Hubert"},{"full_name":"Dovey, Oliver","last_name":"Dovey","first_name":"Oliver"},{"full_name":"Zöchbauer-Müller, Sabine","first_name":"Sabine","last_name":"Zöchbauer-Müller"},{"first_name":"Jonathan L.","last_name":"Cooper","full_name":"Cooper, Jonathan L."},{"last_name":"Vassiliou","first_name":"George S.","full_name":"Vassiliou, George S."},{"last_name":"Stoiber","first_name":"Dagmar","full_name":"Stoiber, Dagmar"},{"last_name":"Wieser","first_name":"Rotraud","full_name":"Wieser, Rotraud"},{"first_name":"Gerwin","last_name":"Heller","full_name":"Heller, Gerwin"}],"file_date_updated":"2020-07-14T12:47:34Z"},{"author":[{"full_name":"Fenu, M.","last_name":"Fenu","first_name":"M."},{"full_name":"Bettermann, T.","first_name":"T.","last_name":"Bettermann"},{"full_name":"Vogl, C.","last_name":"Vogl","first_name":"C."},{"orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser","last_name":"Darwish-Miranda","first_name":"Nasser","id":"39CD9926-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schramel, J.","last_name":"Schramel","first_name":"J."},{"full_name":"Jenner, F.","first_name":"F.","last_name":"Jenner"},{"full_name":"Ribitsch, I.","last_name":"Ribitsch","first_name":"I."}],"file_date_updated":"2020-07-14T12:47:42Z","publication_identifier":{"eissn":["20452322"]},"intvolume":" 9","language":[{"iso":"eng"}],"doi":"10.1038/s41598-019-48930-7","publication":"Scientific Reports","publication_status":"published","status":"public","volume":9,"type":"journal_article","citation":{"ama":"Fenu M, Bettermann T, Vogl C, et al. A novel magnet-based scratch method for standardisation of wound-healing assays. Scientific Reports. 2019;9(1). doi:10.1038/s41598-019-48930-7","ista":"Fenu M, Bettermann T, Vogl C, Darwish-Miranda N, Schramel J, Jenner F, Ribitsch I. 2019. A novel magnet-based scratch method for standardisation of wound-healing assays. Scientific Reports. 9(1), 12625.","chicago":"Fenu, M., T. Bettermann, C. Vogl, Nasser Darwish-Miranda, J. Schramel, F. Jenner, and I. Ribitsch. “A Novel Magnet-Based Scratch Method for Standardisation of Wound-Healing Assays.” Scientific Reports. Springer Nature, 2019. https://doi.org/10.1038/s41598-019-48930-7.","mla":"Fenu, M., et al. “A Novel Magnet-Based Scratch Method for Standardisation of Wound-Healing Assays.” Scientific Reports, vol. 9, no. 1, 12625, Springer Nature, 2019, doi:10.1038/s41598-019-48930-7.","ieee":"M. Fenu et al., “A novel magnet-based scratch method for standardisation of wound-healing assays,” Scientific Reports, vol. 9, no. 1. Springer Nature, 2019.","short":"M. Fenu, T. Bettermann, C. Vogl, N. Darwish-Miranda, J. Schramel, F. Jenner, I. Ribitsch, Scientific Reports 9 (2019).","apa":"Fenu, M., Bettermann, T., Vogl, C., Darwish-Miranda, N., Schramel, J., Jenner, F., & Ribitsch, I. (2019). A novel magnet-based scratch method for standardisation of wound-healing assays. Scientific Reports. Springer Nature. https://doi.org/10.1038/s41598-019-48930-7"},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_number":"12625","month":"09","date_published":"2019-09-02T00:00:00Z","_id":"6867","department":[{"_id":"Bio"}],"isi":1,"day":"02","date_updated":"2023-08-29T07:55:15Z","year":"2019","date_created":"2019-09-15T22:00:42Z","oa":1,"ddc":["570"],"oa_version":"Published Version","quality_controlled":"1","publisher":"Springer Nature","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"A novel magnet-based scratch method for standardisation of wound-healing assays","issue":"1","article_processing_charge":"No","has_accepted_license":"1","file":[{"file_id":"6879","content_type":"application/pdf","date_created":"2019-09-16T12:42:40Z","file_name":"2019_ScientificReports_Fenu.pdf","date_updated":"2020-07-14T12:47:42Z","creator":"dernst","checksum":"9cfd986d4108e288cc72276ef047ab0c","file_size":3523795,"access_level":"open_access","relation":"main_file"}],"abstract":[{"text":"A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories.","lang":"eng"}],"scopus_import":"1","external_id":{"isi":["000483697800007"],"pmid":["31477739"]},"pmid":1},{"author":[{"last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"}],"file_date_updated":"2020-07-14T12:47:54Z","doi":"10.3390/bioengineering6040109","publication":"Bioengineering","publication_identifier":{"eissn":["23065354"]},"language":[{"iso":"eng"}],"intvolume":" 6","status":"public","type":"journal_article","volume":6,"publication_status":"published","citation":{"ama":"Merrin J. Frontiers in microfluidics, a teaching resource review. Bioengineering. 2019;6(4). doi:10.3390/bioengineering6040109","ista":"Merrin J. 2019. Frontiers in microfluidics, a teaching resource review. Bioengineering. 6(4), 109.","mla":"Merrin, Jack. “Frontiers in Microfluidics, a Teaching Resource Review.” Bioengineering, vol. 6, no. 4, 109, MDPI, 2019, doi:10.3390/bioengineering6040109.","chicago":"Merrin, Jack. “Frontiers in Microfluidics, a Teaching Resource Review.” Bioengineering. MDPI, 2019. https://doi.org/10.3390/bioengineering6040109.","ieee":"J. Merrin, “Frontiers in microfluidics, a teaching resource review,” Bioengineering, vol. 6, no. 4. MDPI, 2019.","apa":"Merrin, J. (2019). Frontiers in microfluidics, a teaching resource review. Bioengineering. MDPI. https://doi.org/10.3390/bioengineering6040109","short":"J. Merrin, Bioengineering 6 (2019)."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"month":"12","date_published":"2019-12-03T00:00:00Z","article_number":"109","_id":"7225","isi":1,"department":[{"_id":"NanoFab"}],"day":"03","year":"2019","date_updated":"2023-09-06T14:52:49Z","date_created":"2020-01-05T23:00:45Z","oa":1,"article_type":"review","ddc":["620"],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"MDPI","title":"Frontiers in microfluidics, a teaching resource review","quality_controlled":"1","oa_version":"Published Version","issue":"4","has_accepted_license":"1","abstract":[{"lang":"eng","text":"This is a literature teaching resource review for biologically inspired microfluidics courses\r\nor exploring the diverse applications of microfluidics. The structure is around key papers and model\r\norganisms. While courses gradually change over time, a focus remains on understanding how\r\nmicrofluidics has developed as well as what it can and cannot do for researchers. As a primary\r\nstarting point, we cover micro-fluid mechanics principles and microfabrication of devices. A variety\r\nof applications are discussed using model prokaryotic and eukaryotic organisms from the set\r\nof bacteria (Escherichia coli), trypanosomes (Trypanosoma brucei), yeast (Saccharomyces cerevisiae),\r\nslime molds (Physarum polycephalum), worms (Caenorhabditis elegans), flies (Drosophila melangoster),\r\nplants (Arabidopsis thaliana), and mouse immune cells (Mus musculus). Other engineering and\r\nbiochemical methods discussed include biomimetics, organ on a chip, inkjet, droplet microfluidics,\r\nbiotic games, and diagnostics. While we have not yet reached the end-all lab on a chip,\r\nmicrofluidics can still be used effectively for specific applications."}],"file":[{"checksum":"80f1499e2a4caccdf3aa54b137fd99a0","creator":"dernst","date_updated":"2020-07-14T12:47:54Z","relation":"main_file","access_level":"open_access","file_size":2660780,"date_created":"2020-01-07T14:49:59Z","file_name":"2019_Bioengineering_Merrin.pdf","file_id":"7243","content_type":"application/pdf"}],"article_processing_charge":"Yes","scopus_import":"1","external_id":{"pmid":["31816954"],"isi":["000505590000024"]},"pmid":1},{"day":"15","date_updated":"2023-09-06T15:27:29Z","year":"2019","month":"01","date_published":"2019-01-15T00:00:00Z","_id":"7406","isi":1,"department":[{"_id":"HaJa"},{"_id":"Bio"}],"publication_status":"published","status":"public","volume":312,"type":"journal_article","citation":{"mla":"Mckenzie, Catherine, et al. “Isolation of Synaptic Vesicles from Genetically Engineered Cultured Neurons.” Journal of Neuroscience Methods, vol. 312, Elsevier, 2019, pp. 114–21, doi:10.1016/j.jneumeth.2018.11.018.","chicago":"Mckenzie, Catherine, Miroslava Spanova, Alexander J Johnson, Stephanie Kainrath, Vanessa Zheden, Harald H. Sitte, and Harald L Janovjak. “Isolation of Synaptic Vesicles from Genetically Engineered Cultured Neurons.” Journal of Neuroscience Methods. Elsevier, 2019. https://doi.org/10.1016/j.jneumeth.2018.11.018.","ama":"Mckenzie C, Spanova M, Johnson AJ, et al. Isolation of synaptic vesicles from genetically engineered cultured neurons. Journal of Neuroscience Methods. 2019;312:114-121. doi:10.1016/j.jneumeth.2018.11.018","ista":"Mckenzie C, Spanova M, Johnson AJ, Kainrath S, Zheden V, Sitte HH, Janovjak HL. 2019. Isolation of synaptic vesicles from genetically engineered cultured neurons. Journal of Neuroscience Methods. 312, 114–121.","apa":"Mckenzie, C., Spanova, M., Johnson, A. J., Kainrath, S., Zheden, V., Sitte, H. H., & Janovjak, H. L. (2019). Isolation of synaptic vesicles from genetically engineered cultured neurons. Journal of Neuroscience Methods. Elsevier. https://doi.org/10.1016/j.jneumeth.2018.11.018","short":"C. Mckenzie, M. Spanova, A.J. Johnson, S. Kainrath, V. Zheden, H.H. Sitte, H.L. Janovjak, Journal of Neuroscience Methods 312 (2019) 114–121.","ieee":"C. Mckenzie et al., “Isolation of synaptic vesicles from genetically engineered cultured neurons,” Journal of Neuroscience Methods, vol. 312. Elsevier, pp. 114–121, 2019."},"author":[{"full_name":"Mckenzie, Catherine","last_name":"Mckenzie","id":"3EEDE19A-F248-11E8-B48F-1D18A9856A87","first_name":"Catherine"},{"first_name":"Miroslava","id":"44A924DC-F248-11E8-B48F-1D18A9856A87","last_name":"Spanova","full_name":"Spanova, Miroslava"},{"id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson","full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843"},{"full_name":"Kainrath, Stephanie","id":"32CFBA64-F248-11E8-B48F-1D18A9856A87","first_name":"Stephanie","last_name":"Kainrath"},{"last_name":"Zheden","first_name":"Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa"},{"first_name":"Harald H.","last_name":"Sitte","full_name":"Sitte, Harald H."},{"full_name":"Janovjak, Harald L","orcid":"0000-0002-8023-9315","first_name":"Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak"}],"publication_identifier":{"issn":["0165-0270"]},"intvolume":" 312","language":[{"iso":"eng"}],"doi":"10.1016/j.jneumeth.2018.11.018","publication":"Journal of Neuroscience Methods","scopus_import":"1","external_id":{"isi":["000456220900013"],"pmid":["30496761"]},"pmid":1,"project":[{"name":"Microbial Ion Channels for Synthetic Neurobiology","call_identifier":"FP7","grant_number":"303564","_id":"25548C20-B435-11E9-9278-68D0E5697425"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425"},{"grant_number":"W1232-B24","_id":"2548AE96-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular Drug Targets"}],"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Background\r\nSynaptic vesicles (SVs) are an integral part of the neurotransmission machinery, and isolation of SVs from their host neuron is necessary to reveal their most fundamental biochemical and functional properties in in vitro assays. Isolated SVs from neurons that have been genetically engineered, e.g. to introduce genetically encoded indicators, are not readily available but would permit new insights into SV structure and function. Furthermore, it is unclear if cultured neurons can provide sufficient starting material for SV isolation procedures.\r\n\r\nNew method\r\nHere, we demonstrate an efficient ex vivo procedure to obtain functional SVs from cultured rat cortical neurons after genetic engineering with a lentivirus.\r\n\r\nResults\r\nWe show that ∼108 plated cortical neurons allow isolation of suitable SV amounts for functional analysis and imaging. We found that SVs isolated from cultured neurons have neurotransmitter uptake comparable to that of SVs isolated from intact cortex. Using total internal reflection fluorescence (TIRF) microscopy, we visualized an exogenous SV-targeted marker protein and demonstrated the high efficiency of SV modification.\r\n\r\nComparison with existing methods\r\nObtaining SVs from genetically engineered neurons currently generally requires the availability of transgenic animals, which is constrained by technical (e.g. cost and time) and biological (e.g. developmental defects and lethality) limitations.\r\n\r\nConclusions\r\nThese results demonstrate the modification and isolation of functional SVs using cultured neurons and viral transduction. The ability to readily obtain SVs from genetically engineered neurons will permit linking in situ studies to in vitro experiments in a variety of genetic contexts."}],"page":"114-121","quality_controlled":"1","oa_version":"None","acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Elsevier","title":"Isolation of synaptic vesicles from genetically engineered cultured neurons","ec_funded":1,"date_created":"2020-01-30T09:12:19Z","article_type":"original"},{"author":[{"full_name":"Morandell, Jasmin","first_name":"Jasmin","id":"4739D480-F248-11E8-B48F-1D18A9856A87","last_name":"Morandell"},{"full_name":"Nicolas, Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","first_name":"Armel","last_name":"Nicolas"},{"last_name":"Schwarz","id":"29A8453C-F248-11E8-B48F-1D18A9856A87","first_name":"Lena A","full_name":"Schwarz, Lena A"},{"last_name":"Novarino","first_name":"Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia"}],"date_created":"2020-01-30T10:07:41Z","publication_identifier":{"issn":["0924-977X"]},"article_type":"original","intvolume":" 29","language":[{"iso":"eng"}],"doi":"10.1016/j.euroneuro.2019.09.040","publication":"European Neuropsychopharmacology","publication_status":"published","oa_version":"None","quality_controlled":"1","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Elsevier","title":"S.16.05 Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development and autism","type":"journal_article","volume":29,"citation":{"ama":"Morandell J, Nicolas A, Schwarz LA, Novarino G. S.16.05 Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development and autism. European Neuropsychopharmacology. 2019;29(Supplement 6):S11-S12. doi:10.1016/j.euroneuro.2019.09.040","ista":"Morandell J, Nicolas A, Schwarz LA, Novarino G. 2019. S.16.05 Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development and autism. European Neuropsychopharmacology. 29(Supplement 6), S11–S12.","mla":"Morandell, Jasmin, et al. “S.16.05 Illuminating the Role of the E3 Ubiquitin Ligase Cullin3 in Brain Development and Autism.” European Neuropsychopharmacology, vol. 29, no. Supplement 6, Elsevier, 2019, pp. S11–12, doi:10.1016/j.euroneuro.2019.09.040.","chicago":"Morandell, Jasmin, Armel Nicolas, Lena A Schwarz, and Gaia Novarino. “S.16.05 Illuminating the Role of the E3 Ubiquitin Ligase Cullin3 in Brain Development and Autism.” European Neuropsychopharmacology. Elsevier, 2019. https://doi.org/10.1016/j.euroneuro.2019.09.040.","ieee":"J. Morandell, A. Nicolas, L. A. Schwarz, and G. Novarino, “S.16.05 Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development and autism,” European Neuropsychopharmacology, vol. 29, no. Supplement 6. Elsevier, pp. S11–S12, 2019.","apa":"Morandell, J., Nicolas, A., Schwarz, L. A., & Novarino, G. (2019). S.16.05 Illuminating the role of the e3 ubiquitin ligase cullin3 in brain development and autism. European Neuropsychopharmacology. Elsevier. https://doi.org/10.1016/j.euroneuro.2019.09.040","short":"J. Morandell, A. Nicolas, L.A. Schwarz, G. Novarino, European Neuropsychopharmacology 29 (2019) S11–S12."},"issue":"Supplement 6","month":"12","date_published":"2019-12-13T00:00:00Z","_id":"7415","isi":1,"article_processing_charge":"No","department":[{"_id":"GaNo"},{"_id":"LifeSc"}],"page":"S11-S12","day":"13","date_updated":"2023-09-07T14:56:17Z","external_id":{"isi":["000502657500021"]},"year":"2019"},{"file":[{"file_id":"6096","content_type":"application/pdf","file_name":"2019_PLoSOne_Goudarzi.pdf","date_created":"2019-03-11T16:09:23Z","access_level":"open_access","relation":"main_file","file_size":2967731,"creator":"dernst","checksum":"b885de050ed4bb3c86f706487a47197f","date_updated":"2020-07-14T12:47:19Z"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells results primarily from redistribution of material within the cell rather than being driven by flow of water from an external source."}],"article_processing_charge":"No","scopus_import":"1","external_id":{"isi":["000459712100022"]},"date_created":"2019-03-10T22:59:21Z","ddc":["570"],"oa":1,"title":"Fluid dynamics during bleb formation in migrating cells in vivo","publisher":"Public Library of Science","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","quality_controlled":"1","oa_version":"Published Version","issue":"2","date_published":"2019-02-26T00:00:00Z","month":"02","article_number":"e0212699","department":[{"_id":"Bio"}],"isi":1,"_id":"6093","day":"26","year":"2019","date_updated":"2023-09-19T14:46:47Z","file_date_updated":"2020-07-14T12:47:19Z","author":[{"full_name":"Goudarzi, Mohammad","id":"3384113A-F248-11E8-B48F-1D18A9856A87","first_name":"Mohammad","last_name":"Goudarzi"},{"full_name":"Boquet-Pujadas, Aleix","last_name":"Boquet-Pujadas","first_name":"Aleix"},{"full_name":"Olivo-Marin, Jean Christophe","first_name":"Jean Christophe","last_name":"Olivo-Marin"},{"full_name":"Raz, Erez","last_name":"Raz","first_name":"Erez"}],"publication":"PLOS ONE","doi":"10.1371/journal.pone.0212699","intvolume":" 14","language":[{"iso":"eng"}],"type":"journal_article","volume":14,"status":"public","publication_status":"published","citation":{"apa":"Goudarzi, M., Boquet-Pujadas, A., Olivo-Marin, J. C., & Raz, E. (2019). Fluid dynamics during bleb formation in migrating cells in vivo. PLOS ONE. Public Library of Science. https://doi.org/10.1371/journal.pone.0212699","short":"M. Goudarzi, A. Boquet-Pujadas, J.C. Olivo-Marin, E. Raz, PLOS ONE 14 (2019).","ieee":"M. Goudarzi, A. Boquet-Pujadas, J. C. Olivo-Marin, and E. Raz, “Fluid dynamics during bleb formation in migrating cells in vivo,” PLOS ONE, vol. 14, no. 2. Public Library of Science, 2019.","mla":"Goudarzi, Mohammad, et al. “Fluid Dynamics during Bleb Formation in Migrating Cells in Vivo.” PLOS ONE, vol. 14, no. 2, e0212699, Public Library of Science, 2019, doi:10.1371/journal.pone.0212699.","chicago":"Goudarzi, Mohammad, Aleix Boquet-Pujadas, Jean Christophe Olivo-Marin, and Erez Raz. “Fluid Dynamics during Bleb Formation in Migrating Cells in Vivo.” PLOS ONE. Public Library of Science, 2019. https://doi.org/10.1371/journal.pone.0212699.","ama":"Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E. Fluid dynamics during bleb formation in migrating cells in vivo. PLOS ONE. 2019;14(2). doi:10.1371/journal.pone.0212699","ista":"Goudarzi M, Boquet-Pujadas A, Olivo-Marin JC, Raz E. 2019. Fluid dynamics during bleb formation in migrating cells in vivo. PLOS ONE. 14(2), e0212699."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"}},{"author":[{"id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","first_name":"Patrick","last_name":"Danowski","full_name":"Danowski, Patrick","orcid":"0000-0002-6026-4409"}],"file_date_updated":"2020-07-14T12:47:35Z","doi":"10.31263/voebm.v72i1.2276","publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","publication_identifier":{"eissn":["1022-2588"]},"language":[{"iso":"eng"}],"intvolume":" 72","status":"public","type":"journal_article","volume":72,"publication_status":"published","citation":{"ieee":"P. Danowski, “An Austrian proposal for the classification of Open Access Tuples (COAT) - distinguish different open access types beyond colors,” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol. 72, no. 1. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, pp. 59–65, 2019.","apa":"Danowski, P. (2019). An Austrian proposal for the classification of Open Access Tuples (COAT) - distinguish different open access types beyond colors. Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. https://doi.org/10.31263/voebm.v72i1.2276","short":"P. Danowski, Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare 72 (2019) 59–65.","ama":"Danowski P. An Austrian proposal for the classification of Open Access Tuples (COAT) - distinguish different open access types beyond colors. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 2019;72(1):59-65. doi:10.31263/voebm.v72i1.2276","ista":"Danowski P. 2019. An Austrian proposal for the classification of Open Access Tuples (COAT) - distinguish different open access types beyond colors. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. 72(1), 59–65.","mla":"Danowski, Patrick. “An Austrian Proposal for the Classification of Open Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors.” Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare, vol. 72, no. 1, Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2019, pp. 59–65, doi:10.31263/voebm.v72i1.2276.","chicago":"Danowski, Patrick. “An Austrian Proposal for the Classification of Open Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors.” Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2019. https://doi.org/10.31263/voebm.v72i1.2276."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"month":"05","date_published":"2019-05-17T00:00:00Z","_id":"6657","department":[{"_id":"E-Lib"}],"day":"17","related_material":{"record":[{"relation":"earlier_version","id":"5686","status":"public"}]},"year":"2019","date_updated":"2023-10-17T11:33:58Z","date_created":"2019-07-21T21:59:15Z","oa":1,"ddc":["020"],"article_type":"original","publisher":"Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"An Austrian proposal for the classification of Open Access Tuples (COAT) - distinguish different open access types beyond colors","oa_version":"Published Version","quality_controlled":"1","issue":"1","page":"59-65","file":[{"date_updated":"2020-07-14T12:47:35Z","checksum":"c0d2695d6d0d34e62ba06fb3f0ebaaed","creator":"apreinsp","file_size":468558,"relation":"main_file","access_level":"open_access","file_id":"6661","content_type":"application/pdf","file_name":"2019_MitteilungenDerVOEB_Danowski.pdf","date_created":"2019-07-22T08:45:03Z"}],"has_accepted_license":"1","abstract":[{"text":"In this article a model is described how Open Access definitions can be formed on the basis of objective criteria. The common Open Access definitions such as \"gold\" and \"green\" are not exactly defined. This becomes a problem as soon as one begins to measure Open Access, for example if the development of the Open Access share should be monitored. This was discussed in the working group on Open Access Monitoring of the AT2OA project and the present model was developed, which is based on 5 critics with 4 characteristics: location, licence, version, embargo and conditions of the Open Access publication are taken into account. In the meantime, the model has also been tested in practice using R scripts, and the initial results are quite promising.","lang":"eng"}],"article_processing_charge":"No","scopus_import":"1"},{"month":"04","date_published":"2019-04-25T00:00:00Z","_id":"6328","isi":1,"department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"day":"25","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"14697"},{"relation":"dissertation_contains","id":"6891","status":"public"}],"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/leukocytes-use-their-nucleus-as-a-ruler-to-choose-path-of-least-resistance/"}]},"year":"2019","date_updated":"2024-03-18T23:30:41Z","author":[{"first_name":"Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz","full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","first_name":"Aglaja","last_name":"Kopf","full_name":"Kopf, Aglaja","orcid":"0000-0002-2187-6656"},{"last_name":"Stopp","first_name":"Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87","full_name":"Stopp, Julian A"},{"full_name":"de Vries, Ingrid","last_name":"de Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid"},{"full_name":"Driscoll, Meghan K.","last_name":"Driscoll","first_name":"Meghan K."},{"last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"full_name":"Welf, Erik S.","first_name":"Erik S.","last_name":"Welf"},{"full_name":"Danuser, Gaudenz","first_name":"Gaudenz","last_name":"Danuser"},{"full_name":"Fiolka, Reto","last_name":"Fiolka","first_name":"Reto"},{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"doi":"10.1038/s41586-019-1087-5","publication":"Nature","intvolume":" 568","language":[{"iso":"eng"}],"status":"public","type":"journal_article","volume":568,"publication_status":"published","citation":{"apa":"Renkawitz, J., Kopf, A., Stopp, J. A., de Vries, I., Driscoll, M. K., Merrin, J., … Sixt, M. K. (2019). Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature. Springer Nature. https://doi.org/10.1038/s41586-019-1087-5","short":"J. Renkawitz, A. Kopf, J.A. Stopp, I. de Vries, M.K. Driscoll, J. Merrin, R. Hauschild, E.S. Welf, G. Danuser, R. Fiolka, M.K. Sixt, Nature 568 (2019) 546–550.","ieee":"J. Renkawitz et al., “Nuclear positioning facilitates amoeboid migration along the path of least resistance,” Nature, vol. 568. Springer Nature, pp. 546–550, 2019.","mla":"Renkawitz, Jörg, et al. “Nuclear Positioning Facilitates Amoeboid Migration along the Path of Least Resistance.” Nature, vol. 568, Springer Nature, 2019, pp. 546–50, doi:10.1038/s41586-019-1087-5.","chicago":"Renkawitz, Jörg, Aglaja Kopf, Julian A Stopp, Ingrid de Vries, Meghan K. Driscoll, Jack Merrin, Robert Hauschild, et al. “Nuclear Positioning Facilitates Amoeboid Migration along the Path of Least Resistance.” Nature. Springer Nature, 2019. https://doi.org/10.1038/s41586-019-1087-5.","ista":"Renkawitz J, Kopf A, Stopp JA, de Vries I, Driscoll MK, Merrin J, Hauschild R, Welf ES, Danuser G, Fiolka R, Sixt MK. 2019. Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature. 568, 546–550.","ama":"Renkawitz J, Kopf A, Stopp JA, et al. Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature. 2019;568:546-550. doi:10.1038/s41586-019-1087-5"},"abstract":[{"text":"During metazoan development, immune surveillance and cancer dissemination, cells migrate in complex three-dimensional microenvironments1,2,3. These spaces are crowded by cells and extracellular matrix, generating mazes with differently sized gaps that are typically smaller than the diameter of the migrating cell4,5. Most mesenchymal and epithelial cells and some—but not all—cancer cells actively generate their migratory path using pericellular tissue proteolysis6. By contrast, amoeboid cells such as leukocytes use non-destructive strategies of locomotion7, raising the question how these extremely fast cells navigate through dense tissues. Here we reveal that leukocytes sample their immediate vicinity for large pore sizes, and are thereby able to choose the path of least resistance. This allows them to circumnavigate local obstacles while effectively following global directional cues such as chemotactic gradients. Pore-size discrimination is facilitated by frontward positioning of the nucleus, which enables the cells to use their bulkiest compartment as a mechanical gauge. Once the nucleus and the closely associated microtubule organizing centre pass the largest pore, cytoplasmic protrusions still lingering in smaller pores are retracted. These retractions are coordinated by dynamic microtubules; when microtubules are disrupted, migrating cells lose coherence and frequently fragment into migratory cytoplasmic pieces. As nuclear positioning in front of the microtubule organizing centre is a typical feature of amoeboid migration, our findings link the fundamental organization of cellular polarity to the strategy of locomotion.","lang":"eng"}],"page":"546-550","project":[{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7"},{"grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","call_identifier":"H2020"},{"grant_number":"W01250-B20","_id":"265FAEBA-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","call_identifier":"FWF"},{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"_id":"25A48D24-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 1396-2014","name":"Molecular and system level view of immune cell migration"}],"article_processing_charge":"No","scopus_import":"1","external_id":{"isi":["000465594200050"],"pmid":["30944468"]},"pmid":1,"ec_funded":1,"date_created":"2019-04-17T06:52:28Z","oa":1,"article_type":"letter_note","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Springer Nature","title":"Nuclear positioning facilitates amoeboid migration along the path of least resistance","quality_controlled":"1","oa_version":"Submitted Version","acknowledged_ssus":[{"_id":"SSU"}],"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217284/","open_access":"1"}]},{"publisher":"Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"IST PubRep and IST DataRep: the institutional repositories at IST Austria","oa_version":"Published Version","issue":"1","date_created":"2018-12-11T11:44:22Z","oa":1,"ddc":["020"],"scopus_import":1,"abstract":[{"text":"In 2013, a publication repository was implemented at IST Austria and 2015 after a thorough preparation phase a data repository was implemented - both based on the Open Source Software EPrints. In this text, designed as field report, we will reflect on our experiences with Open Source Software in general and specifically with EPrints regarding technical aspects but also regarding their characteristics of the user community. The second part is a pleading for including the end users in the process of implementation, adaption and evaluation.","lang":"eng"}],"file":[{"creator":"dernst","checksum":"7ac61bade5f37db011ca435ebcf86797","date_updated":"2020-07-14T12:46:38Z","access_level":"open_access","relation":"main_file","file_size":509434,"content_type":"application/pdf","file_id":"5702","date_created":"2018-12-17T12:40:27Z","file_name":"2018_VOEB_Petritsch.pdf"}],"page":"199 - 206","has_accepted_license":"1","status":"public","type":"journal_article","volume":71,"publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"B. Petritsch and J. Porsche, “IST PubRep and IST DataRep: the institutional repositories at IST Austria,” VÖB Mitteilungen, vol. 71, no. 1. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, pp. 199–206, 2018.","apa":"Petritsch, B., & Porsche, J. (2018). IST PubRep and IST DataRep: the institutional repositories at IST Austria. VÖB Mitteilungen. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare. https://doi.org/10.31263/voebm.v71i1.1993","short":"B. Petritsch, J. Porsche, VÖB Mitteilungen 71 (2018) 199–206.","ama":"Petritsch B, Porsche J. IST PubRep and IST DataRep: the institutional repositories at IST Austria. VÖB Mitteilungen. 2018;71(1):199-206. doi:10.31263/voebm.v71i1.1993","ista":"Petritsch B, Porsche J. 2018. IST PubRep and IST DataRep: the institutional repositories at IST Austria. VÖB Mitteilungen. 71(1), 199–206.","mla":"Petritsch, Barbara, and Jana Porsche. “IST PubRep and IST DataRep: The Institutional Repositories at IST Austria.” VÖB Mitteilungen, vol. 71, no. 1, Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2018, pp. 199–206, doi:10.31263/voebm.v71i1.1993.","chicago":"Petritsch, Barbara, and Jana Porsche. “IST PubRep and IST DataRep: The Institutional Repositories at IST Austria.” VÖB Mitteilungen. Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, 2018. https://doi.org/10.31263/voebm.v71i1.1993."},"publist_id":"8001","author":[{"id":"406048EC-F248-11E8-B48F-1D18A9856A87","first_name":"Barbara","last_name":"Petritsch","full_name":"Petritsch, Barbara","orcid":"0000-0003-2724-4614"},{"last_name":"Porsche","id":"3252EDC2-F248-11E8-B48F-1D18A9856A87","first_name":"Jana","full_name":"Porsche, Jana"}],"file_date_updated":"2020-07-14T12:46:38Z","doi":"10.31263/voebm.v71i1.1993","publication":"VÖB Mitteilungen","intvolume":" 71","language":[{"iso":"eng"}],"day":"01","year":"2018","date_updated":"2021-01-12T08:01:26Z","month":"10","date_published":"2018-10-01T00:00:00Z","_id":"53","department":[{"_id":"E-Lib"}]},{"year":"2018","date_updated":"2020-07-14T23:06:21Z","day":"24","file":[{"date_created":"2019-05-16T07:26:25Z","file_name":"Poster_Beitrag_125_Petritsch.pdf","file_id":"6460","content_type":"application/pdf","checksum":"9063ab4d10ea93353c3a03bbf53fbcf1","creator":"dernst","date_updated":"2020-07-14T12:47:30Z","access_level":"open_access","relation":"main_file","file_size":1967778}],"has_accepted_license":"1","_id":"6459","department":[{"_id":"E-Lib"}],"month":"09","date_published":"2018-09-24T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"short":"B. Petritsch, Open Access at IST Austria 2009-2017, IST Austria, 2018.","apa":"Petritsch, B. (2018). Open Access at IST Austria 2009-2017. Presented at the Open-Access-Tage, Graz, Austria: IST Austria. https://doi.org/10.5281/zenodo.1410279","ieee":"B. Petritsch, Open Access at IST Austria 2009-2017. IST Austria, 2018.","chicago":"Petritsch, Barbara. Open Access at IST Austria 2009-2017. IST Austria, 2018. https://doi.org/10.5281/zenodo.1410279.","mla":"Petritsch, Barbara. Open Access at IST Austria 2009-2017. IST Austria, 2018, doi:10.5281/zenodo.1410279.","ista":"Petritsch B. 2018. Open Access at IST Austria 2009-2017, IST Austria,p.","ama":"Petritsch B. Open Access at IST Austria 2009-2017. IST Austria; 2018. doi:10.5281/zenodo.1410279"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"IST Austria","status":"public","title":"Open Access at IST Austria 2009-2017","type":"conference_poster","publication_status":"published","oa_version":"Published Version","conference":{"end_date":"2018-09-26","location":"Graz, Austria","start_date":"2018-09-24","name":"Open-Access-Tage"},"doi":"10.5281/zenodo.1410279","oa":1,"language":[{"iso":"eng"}],"ddc":["020"],"keyword":["Open Access","Publication Analysis"],"date_created":"2019-05-16T07:27:14Z","author":[{"last_name":"Petritsch","id":"406048EC-F248-11E8-B48F-1D18A9856A87","first_name":"Barbara","orcid":"0000-0003-2724-4614","full_name":"Petritsch, Barbara"}],"file_date_updated":"2020-07-14T12:47:30Z"},{"author":[{"orcid":"0000-0001-7190-0776","full_name":"Ratheesh, Aparna","last_name":"Ratheesh","id":"2F064CFE-F248-11E8-B48F-1D18A9856A87","first_name":"Aparna"},{"last_name":"Biebl","id":"3CCBB46E-F248-11E8-B48F-1D18A9856A87","first_name":"Julia","full_name":"Biebl, Julia"},{"first_name":"Michael","last_name":"Smutny","full_name":"Smutny, Michael"},{"full_name":"Veselá, Jana","id":"433253EE-F248-11E8-B48F-1D18A9856A87","first_name":"Jana","last_name":"Veselá"},{"id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina","last_name":"Papusheva","full_name":"Papusheva, Ekaterina"},{"last_name":"Krens","first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter"},{"last_name":"György","first_name":"Attila","id":"3BCEDBE0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1819-198X","full_name":"György, Attila"},{"first_name":"Alessandra M","id":"3DBA3F4E-F248-11E8-B48F-1D18A9856A87","last_name":"Casano","full_name":"Casano, Alessandra M","orcid":"0000-0002-6009-6804"},{"full_name":"Siekhaus, Daria E","orcid":"0000-0001-8323-8353","first_name":"Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus"}],"doi":"10.1016/j.devcel.2018.04.002","publication":"Developmental Cell","language":[{"iso":"eng"}],"intvolume":" 45","status":"public","type":"journal_article","volume":45,"publication_status":"published","citation":{"mla":"Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” Developmental Cell, vol. 45, no. 3, Elsevier, 2018, pp. 331–46, doi:10.1016/j.devcel.2018.04.002.","chicago":"Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano, and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration.” Developmental Cell. Elsevier, 2018. https://doi.org/10.1016/j.devcel.2018.04.002.","ista":"Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W, György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 45(3), 331–346.","ama":"Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. 2018;45(3):331-346. doi:10.1016/j.devcel.2018.04.002","apa":"Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G., … Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration. Developmental Cell. Elsevier. https://doi.org/10.1016/j.devcel.2018.04.002","short":"A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W. Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018) 331–346.","ieee":"A. Ratheesh et al., “Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration,” Developmental Cell, vol. 45, no. 3. Elsevier, pp. 331–346, 2018."},"month":"05","date_published":"2018-05-07T00:00:00Z","_id":"308","department":[{"_id":"DaSi"},{"_id":"CaHe"},{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"MiSi"}],"isi":1,"day":"07","related_material":{"link":[{"url":"https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/","description":"News on IST Homepage","relation":"press_release"}]},"year":"2018","date_updated":"2023-09-11T13:22:13Z","ec_funded":1,"date_created":"2018-12-11T11:45:44Z","oa":1,"article_type":"original","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Elsevier","title":"Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration","quality_controlled":"1","oa_version":"Published Version","acknowledged_ssus":[{"_id":"SSU"}],"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2018.04.002","open_access":"1"}],"issue":"3","page":"331 - 346","abstract":[{"text":"Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo.","lang":"eng"}],"project":[{"call_identifier":"FWF","name":"Drosophila TNFa´s Funktion in Immunzellen","grant_number":"P29638","_id":"253B6E48-B435-11E9-9278-68D0E5697425"},{"name":"Investigating the role of transporters in invasive migration through junctions","call_identifier":"FP7","_id":"2536F660-B435-11E9-9278-68D0E5697425","grant_number":"334077"}],"article_processing_charge":"No","scopus_import":"1","external_id":{"isi":["000432461400009"],"pmid":["29738712"]},"pmid":1},{"year":"2018","date_updated":"2023-09-11T14:01:18Z","day":"13","_id":"437","isi":1,"department":[{"_id":"MiSi"},{"_id":"Bio"}],"month":"02","date_published":"2018-02-13T00:00:00Z","citation":{"mla":"Leithner, Alexander F., et al. “Fast and Efficient Genetic Engineering of Hematopoietic Precursor Cells for the Study of Dendritic Cell Migration.” European Journal of Immunology, vol. 48, no. 6, Wiley-Blackwell, 2018, pp. 1074–77, doi:10.1002/eji.201747358.","chicago":"Leithner, Alexander F, Jörg Renkawitz, Ingrid de Vries, Robert Hauschild, Hans Haecker, and Michael K Sixt. “Fast and Efficient Genetic Engineering of Hematopoietic Precursor Cells for the Study of Dendritic Cell Migration.” European Journal of Immunology. Wiley-Blackwell, 2018. https://doi.org/10.1002/eji.201747358.","ama":"Leithner AF, Renkawitz J, de Vries I, Hauschild R, Haecker H, Sixt MK. Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration. European Journal of Immunology. 2018;48(6):1074-1077. doi:10.1002/eji.201747358","ista":"Leithner AF, Renkawitz J, de Vries I, Hauschild R, Haecker H, Sixt MK. 2018. Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration. European Journal of Immunology. 48(6), 1074–1077.","short":"A.F. Leithner, J. Renkawitz, I. de Vries, R. Hauschild, H. Haecker, M.K. Sixt, European Journal of Immunology 48 (2018) 1074–1077.","apa":"Leithner, A. F., Renkawitz, J., de Vries, I., Hauschild, R., Haecker, H., & Sixt, M. K. (2018). Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration. European Journal of Immunology. Wiley-Blackwell. https://doi.org/10.1002/eji.201747358","ieee":"A. F. Leithner, J. Renkawitz, I. de Vries, R. Hauschild, H. Haecker, and M. K. Sixt, “Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration,” European Journal of Immunology, vol. 48, no. 6. Wiley-Blackwell, pp. 1074–1077, 2018."},"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)"},"status":"public","type":"journal_article","volume":48,"publication_status":"published","pubrep_id":"1067","acknowledgement":"This work was supported by grants of the European Research Council (ERC CoG 724373) and the Austrian Science Fund (FWF) to M.S. We thank the scientific support units at IST Austria for excellent technical support.\r\nWe thank the scientific support units at IST Austria for excellent technical support. ","doi":"10.1002/eji.201747358","publication":"European Journal of Immunology","intvolume":" 48","language":[{"iso":"eng"}],"publist_id":"7386","author":[{"orcid":"0000-0002-1073-744X","full_name":"Leithner, Alexander F","last_name":"Leithner","first_name":"Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369","first_name":"Jörg","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz"},{"full_name":"De Vries, Ingrid","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","last_name":"De Vries"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"full_name":"Haecker, Hans","last_name":"Haecker","first_name":"Hans"},{"orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2020-07-14T12:46:27Z","external_id":{"isi":["000434963700016"]},"scopus_import":"1","license":"https://creativecommons.org/licenses/by-nc/4.0/","file":[{"file_id":"5044","content_type":"application/pdf","file_name":"IST-2018-1067-v1+2_Leithner_et_al-2018-European_Journal_of_Immunology.pdf","date_created":"2018-12-12T10:13:56Z","date_updated":"2020-07-14T12:46:27Z","creator":"system","checksum":"9d5b74cd016505aeb9a4c2d33bbedaeb","file_size":590106,"relation":"main_file","access_level":"open_access"}],"has_accepted_license":"1","page":"1074 - 1077","abstract":[{"text":"Dendritic cells (DCs) are sentinels of the adaptive immune system that reside in peripheral organs of mammals. Upon pathogen encounter, they undergo maturation and up-regulate the chemokine receptor CCR7 that guides them along gradients of its chemokine ligands CCL19 and 21 to the next draining lymph node. There, DCs present peripherally acquired antigen to naïve T cells, thereby triggering adaptive immunity.","lang":"eng"}],"project":[{"name":"Cellular navigation along spatial gradients","call_identifier":"H2020","grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"Yes (via OA deal)","issue":"6","publisher":"Wiley-Blackwell","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Fast and efficient genetic engineering of hematopoietic precursor cells for the study of dendritic cell migration","quality_controlled":"1","oa_version":"Published Version","acknowledged_ssus":[{"_id":"SSU"}],"oa":1,"ddc":["570"],"ec_funded":1,"date_created":"2018-12-11T11:46:28Z"},{"citation":{"ieee":"M. Brown et al., “Lymphatic exosomes promote dendritic cell migration along guidance cues,” Journal of Cell Biology, vol. 217, no. 6. Rockefeller University Press, pp. 2205–2221, 2018.","short":"M. Brown, L. Johnson, D. Leone, P. Májek, K. Vaahtomeri, D. Senfter, N. Bukosza, H. Schachner, G. Asfour, B. Langer, R. Hauschild, K. Parapatics, Y. Hong, K. Bennett, R. Kain, M. Detmar, M.K. Sixt, D. Jackson, D. Kerjaschki, Journal of Cell Biology 217 (2018) 2205–2221.","apa":"Brown, M., Johnson, L., Leone, D., Májek, P., Vaahtomeri, K., Senfter, D., … Kerjaschki, D. (2018). Lymphatic exosomes promote dendritic cell migration along guidance cues. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.201612051","ama":"Brown M, Johnson L, Leone D, et al. Lymphatic exosomes promote dendritic cell migration along guidance cues. Journal of Cell Biology. 2018;217(6):2205-2221. doi:10.1083/jcb.201612051","ista":"Brown M, Johnson L, Leone D, Májek P, Vaahtomeri K, Senfter D, Bukosza N, Schachner H, Asfour G, Langer B, Hauschild R, Parapatics K, Hong Y, Bennett K, Kain R, Detmar M, Sixt MK, Jackson D, Kerjaschki D. 2018. Lymphatic exosomes promote dendritic cell migration along guidance cues. Journal of Cell Biology. 217(6), 2205–2221.","mla":"Brown, Markus, et al. “Lymphatic Exosomes Promote Dendritic Cell Migration along Guidance Cues.” Journal of Cell Biology, vol. 217, no. 6, Rockefeller University Press, 2018, pp. 2205–21, doi:10.1083/jcb.201612051.","chicago":"Brown, Markus, Louise Johnson, Dario Leone, Peter Májek, Kari Vaahtomeri, Daniel Senfter, Nora Bukosza, et al. “Lymphatic Exosomes Promote Dendritic Cell Migration along Guidance Cues.” Journal of Cell Biology. Rockefeller University Press, 2018. https://doi.org/10.1083/jcb.201612051."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"publication_status":"published","type":"journal_article","volume":217,"status":"public","intvolume":" 217","language":[{"iso":"eng"}],"publication":"Journal of Cell Biology","doi":"10.1083/jcb.201612051","acknowledgement":"M. Brown was supported by the Cell Communication in Health and Disease Graduate Study Program of the Austrian Science Fund and Medizinische Universität Wien, M. Sixt by the European Research Council (ERC GA 281556) and an Austrian Science Fund START award, K.L. Bennett by the Austrian Academy of Sciences, D.G. Jackson and L.A. Johnson by Unit Funding (MC_UU_12010/2) and project grants from the Medical Research Council (G1100134 and MR/L008610/1), and M. Detmar by the Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung and Advanced European Research Council grant LYVICAM. K. Vaahtomeri was supported by an Academy of Finland postdoctoral research grant (287853). This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 668036 (RELENT).","file_date_updated":"2020-07-14T12:45:45Z","author":[{"id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","first_name":"Markus","last_name":"Brown","full_name":"Brown, Markus"},{"full_name":"Johnson, Louise","last_name":"Johnson","first_name":"Louise"},{"full_name":"Leone, Dario","first_name":"Dario","last_name":"Leone"},{"last_name":"Májek","first_name":"Peter","full_name":"Májek, Peter"},{"orcid":"0000-0001-7829-3518","full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Senfter, Daniel","first_name":"Daniel","last_name":"Senfter"},{"first_name":"Nora","last_name":"Bukosza","full_name":"Bukosza, Nora"},{"last_name":"Schachner","first_name":"Helga","full_name":"Schachner, Helga"},{"full_name":"Asfour, Gabriele","last_name":"Asfour","first_name":"Gabriele"},{"full_name":"Langer, Brigitte","first_name":"Brigitte","last_name":"Langer"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Katja","last_name":"Parapatics","full_name":"Parapatics, Katja"},{"last_name":"Hong","first_name":"Young","full_name":"Hong, Young"},{"full_name":"Bennett, Keiryn","first_name":"Keiryn","last_name":"Bennett"},{"full_name":"Kain, Renate","first_name":"Renate","last_name":"Kain"},{"last_name":"Detmar","first_name":"Michael","full_name":"Detmar, Michael"},{"orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"full_name":"Jackson, David","last_name":"Jackson","first_name":"David"},{"full_name":"Kerjaschki, Dontscho","last_name":"Kerjaschki","first_name":"Dontscho"}],"publist_id":"7627","date_updated":"2023-09-13T08:51:29Z","year":"2018","day":"12","isi":1,"department":[{"_id":"MiSi"},{"_id":"Bio"}],"_id":"275","date_published":"2018-04-12T00:00:00Z","month":"04","issue":"6","quality_controlled":"1","oa_version":"Published Version","title":"Lymphatic exosomes promote dendritic cell migration along guidance cues","publisher":"Rockefeller University Press","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ddc":["570"],"oa":1,"date_created":"2018-12-11T11:45:33Z","ec_funded":1,"pmid":1,"external_id":{"isi":["000438077800026"],"pmid":["29650776"]},"scopus_import":"1","article_processing_charge":"No","project":[{"_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12","name":"Cytoskeletal force generation and transduction of leukocytes (FWF)","call_identifier":"FWF"},{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendritic cells. In this study, we report that LECs also release basolateral exosome-rich endothelial vesicles (EEVs) that are secreted in greater numbers in the presence of inflammatory cytokines and accumulate in the perivascular stroma of small lymphatic vessels in human chronic inflammatory diseases. Proteomic analyses of EEV fractions identified > 1,700 cargo proteins and revealed a dominant motility-promoting protein signature. In vitro and ex vivo EEV fractions augmented cellular protrusion formation in a CX3CL1/fractalkine-dependent fashion and enhanced the directional migratory response of human dendritic cells along guidance cues. We conclude that perilymphatic LEC exosomes enhance exploratory behavior and thus promote directional migration of CX3CR1-expressing cells in complex tissue environments."}],"file":[{"date_updated":"2020-07-14T12:45:45Z","checksum":"9c7eba51a35c62da8c13f98120b64df4","creator":"dernst","file_size":2252043,"relation":"main_file","access_level":"open_access","file_id":"5704","content_type":"application/pdf","date_created":"2018-12-17T12:50:07Z","file_name":"2018_JournalCellBiology_Brown.pdf"}],"page":"2205 - 2221"},{"oa_version":"None","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Academic Press","title":"Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments","date_created":"2018-12-11T11:44:54Z","external_id":{"pmid":["30165964"],"isi":["000452412300006"]},"pmid":1,"scopus_import":"1","article_processing_charge":"No","abstract":[{"lang":"eng","text":"Cells migrating in multicellular organisms steadily traverse complex three-dimensional (3D) environments. To decipher the underlying cell biology, current experimental setups either use simplified 2D, tissue-mimetic 3D (e.g., collagen matrices) or in vivo environments. While only in vivo experiments are truly physiological, they do not allow for precise manipulation of environmental parameters. 2D in vitro experiments do allow mechanical and chemical manipulations, but increasing evidence demonstrates substantial differences of migratory mechanisms in 2D and 3D. Here, we describe simple, robust, and versatile “pillar forests” to investigate cell migration in complex but fully controllable 3D environments. Pillar forests are polydimethylsiloxane-based setups, in which two closely adjacent surfaces are interconnected by arrays of micrometer-sized pillars. Changing the pillar shape, size, height and the inter-pillar distance precisely manipulates microenvironmental parameters (e.g., pore sizes, micro-geometry, micro-topology), while being easily combined with chemotactic cues, surface coatings, diverse cell types and advanced imaging techniques. Thus, pillar forests combine the advantages of 2D cell migration assays with the precise definition of 3D environmental parameters."}],"page":"79 - 91","citation":{"apa":"Renkawitz, J., Reversat, A., Leithner, A. F., Merrin, J., & Sixt, M. K. (2018). Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments. In Methods in Cell Biology (Vol. 147, pp. 79–91). Academic Press. https://doi.org/10.1016/bs.mcb.2018.07.004","short":"J. Renkawitz, A. Reversat, A.F. Leithner, J. Merrin, M.K. Sixt, in:, Methods in Cell Biology, Academic Press, 2018, pp. 79–91.","ieee":"J. Renkawitz, A. Reversat, A. F. Leithner, J. Merrin, and M. K. Sixt, “Micro-engineered ‘pillar forests’ to study cell migration in complex but controlled 3D environments,” in Methods in Cell Biology, vol. 147, Academic Press, 2018, pp. 79–91.","mla":"Renkawitz, Jörg, et al. “Micro-Engineered ‘Pillar Forests’ to Study Cell Migration in Complex but Controlled 3D Environments.” Methods in Cell Biology, vol. 147, Academic Press, 2018, pp. 79–91, doi:10.1016/bs.mcb.2018.07.004.","chicago":"Renkawitz, Jörg, Anne Reversat, Alexander F Leithner, Jack Merrin, and Michael K Sixt. “Micro-Engineered ‘Pillar Forests’ to Study Cell Migration in Complex but Controlled 3D Environments.” In Methods in Cell Biology, 147:79–91. Academic Press, 2018. https://doi.org/10.1016/bs.mcb.2018.07.004.","ama":"Renkawitz J, Reversat A, Leithner AF, Merrin J, Sixt MK. Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments. In: Methods in Cell Biology. Vol 147. Academic Press; 2018:79-91. doi:10.1016/bs.mcb.2018.07.004","ista":"Renkawitz J, Reversat A, Leithner AF, Merrin J, Sixt MK. 2018.Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments. In: Methods in Cell Biology. vol. 147, 79–91."},"publication_status":"published","status":"public","volume":147,"type":"book_chapter","publication_identifier":{"issn":["0091679X"]},"language":[{"iso":"eng"}],"intvolume":" 147","doi":"10.1016/bs.mcb.2018.07.004","publication":"Methods in Cell Biology","author":[{"orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg","last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","first_name":"Jörg"},{"full_name":"Reversat, Anne","orcid":"0000-0003-0666-8928","first_name":"Anne","id":"35B76592-F248-11E8-B48F-1D18A9856A87","last_name":"Reversat"},{"first_name":"Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","last_name":"Leithner","full_name":"Leithner, Alexander F","orcid":"0000-0002-1073-744X"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","first_name":"Jack","last_name":"Merrin","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609"},{"orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"publist_id":"7768","date_updated":"2023-09-13T08:56:35Z","year":"2018","day":"27","_id":"153","department":[{"_id":"MiSi"},{"_id":"NanoFab"}],"isi":1,"month":"07","date_published":"2018-07-27T00:00:00Z"},{"date_created":"2018-12-11T11:45:07Z","oa":1,"article_type":"original","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Springer Nature","title":"Rapid and reversible root growth inhibition by TIR1 auxin signalling","quality_controlled":"1","oa_version":"Submitted Version","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pubmed/29942048"}],"issue":"7","page":"453 - 459","abstract":[{"lang":"eng","text":"The phytohormone auxin is the information carrier in a plethora of developmental and physiological processes in plants(1). It has been firmly established that canonical, nuclear auxin signalling acts through regulation of gene transcription(2). Here, we combined microfluidics, live imaging, genetic engineering and computational modelling to reanalyse the classical case of root growth inhibition(3) by auxin. We show that Arabidopsis roots react to addition and removal of auxin by extremely rapid adaptation of growth rate. This process requires intracellular auxin perception but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA co-receptor complex is required for the growth regulation, hinting to a novel, non-transcriptional branch of this signalling pathway. Our results challenge the current understanding of root growth regulation by auxin and suggest another, presumably non-transcriptional, signalling output of the canonical auxin pathway."}],"article_processing_charge":"No","scopus_import":"1","external_id":{"pmid":["29942048"],"isi":["000443221200017"]},"pmid":1,"publist_id":"7728","author":[{"last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","first_name":"Matyas","orcid":"0000-0002-9767-8699","full_name":"Fendrych, Matyas"},{"id":"3425EC26-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","last_name":"Akhmanova","full_name":"Akhmanova, Maria","orcid":"0000-0003-1522-3162"},{"orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Glanc, Matous","last_name":"Glanc","first_name":"Matous"},{"first_name":"Shinya","last_name":"Hagihara","full_name":"Hagihara, Shinya"},{"full_name":"Takahashi, Koji","last_name":"Takahashi","first_name":"Koji"},{"last_name":"Uchida","first_name":"Naoyuki","full_name":"Uchida, Naoyuki"},{"full_name":"Torii, Keiko U","last_name":"Torii","first_name":"Keiko U"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"}],"doi":"10.1038/s41477-018-0190-1","publication":"Nature Plants","language":[{"iso":"eng"}],"intvolume":" 4","status":"public","type":"journal_article","volume":4,"publication_status":"published","citation":{"ama":"Fendrych M, Akhmanova M, Merrin J, et al. Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. 2018;4(7):453-459. doi:10.1038/s41477-018-0190-1","ista":"Fendrych M, Akhmanova M, Merrin J, Glanc M, Hagihara S, Takahashi K, Uchida N, Torii KU, Friml J. 2018. Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. 4(7), 453–459.","mla":"Fendrych, Matyas, et al. “Rapid and Reversible Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants, vol. 4, no. 7, Springer Nature, 2018, pp. 453–59, doi:10.1038/s41477-018-0190-1.","chicago":"Fendrych, Matyas, Maria Akhmanova, Jack Merrin, Matous Glanc, Shinya Hagihara, Koji Takahashi, Naoyuki Uchida, Keiko U Torii, and Jiří Friml. “Rapid and Reversible Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants. Springer Nature, 2018. https://doi.org/10.1038/s41477-018-0190-1.","ieee":"M. Fendrych et al., “Rapid and reversible root growth inhibition by TIR1 auxin signalling,” Nature Plants, vol. 4, no. 7. Springer Nature, pp. 453–459, 2018.","short":"M. Fendrych, M. Akhmanova, J. Merrin, M. Glanc, S. Hagihara, K. Takahashi, N. Uchida, K.U. Torii, J. Friml, Nature Plants 4 (2018) 453–459.","apa":"Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi, K., … Friml, J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-018-0190-1"},"month":"06","date_published":"2018-06-25T00:00:00Z","_id":"192","department":[{"_id":"JiFr"},{"_id":"DaSi"},{"_id":"NanoFab"}],"isi":1,"day":"25","related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/new-mechanism-for-the-plant-hormone-auxin-discovered/"}]},"year":"2018","date_updated":"2023-09-15T12:11:03Z"},{"date_published":"2018-12-01T00:00:00Z","month":"12","isi":1,"department":[{"_id":"RySh"},{"_id":"EM-Fac"}],"_id":"163","day":"01","date_updated":"2023-10-17T08:42:24Z","year":"2018","author":[{"first_name":"Siegfried","last_name":"Reipert","full_name":"Reipert, Siegfried"},{"full_name":"Goldammer, Helmuth","first_name":"Helmuth","last_name":"Goldammer"},{"full_name":"Richardson, Christine","last_name":"Richardson","first_name":"Christine"},{"first_name":"Martin","last_name":"Goldberg","full_name":"Goldberg, Martin"},{"first_name":"Timothy","last_name":"Hawkins","full_name":"Hawkins, Timothy"},{"full_name":"Hollergschwandtner, Elena","last_name":"Hollergschwandtner","id":"3C054040-F248-11E8-B48F-1D18A9856A87","first_name":"Elena"},{"first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315"},{"full_name":"Antreich, Sebastian","first_name":"Sebastian","last_name":"Antreich"},{"first_name":"York","last_name":"Stierhof","full_name":"Stierhof, York"}],"language":[{"iso":"eng"}],"intvolume":" 66","publication_identifier":{"issn":["0022-1554"]},"publication":"Journal of Histochemistry and Cytochemistry","doi":"10.1369/0022155418786698","publication_status":"published","volume":66,"type":"journal_article","status":"public","citation":{"mla":"Reipert, Siegfried, et al. “Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.” Journal of Histochemistry and Cytochemistry, vol. 66, no. 12, SAGE Publications, 2018, pp. 903–21, doi:10.1369/0022155418786698.","chicago":"Reipert, Siegfried, Helmuth Goldammer, Christine Richardson, Martin Goldberg, Timothy Hawkins, Elena Saeckl, Walter Kaufmann, Sebastian Antreich, and York Stierhof. “Agitation Modules: Flexible Means to Accelerate Automated Freeze Substitution.” Journal of Histochemistry and Cytochemistry. SAGE Publications, 2018. https://doi.org/10.1369/0022155418786698.","ista":"Reipert S, Goldammer H, Richardson C, Goldberg M, Hawkins T, Saeckl E, Kaufmann W, Antreich S, Stierhof Y. 2018. Agitation modules: Flexible means to accelerate automated freeze substitution. Journal of Histochemistry and Cytochemistry. 66(12), 903–921.","ama":"Reipert S, Goldammer H, Richardson C, et al. Agitation modules: Flexible means to accelerate automated freeze substitution. Journal of Histochemistry and Cytochemistry. 2018;66(12):903-921. doi:10.1369/0022155418786698","short":"S. Reipert, H. Goldammer, C. Richardson, M. Goldberg, T. Hawkins, E. Saeckl, W. Kaufmann, S. Antreich, Y. Stierhof, Journal of Histochemistry and Cytochemistry 66 (2018) 903–921.","apa":"Reipert, S., Goldammer, H., Richardson, C., Goldberg, M., Hawkins, T., Saeckl, E., … Stierhof, Y. (2018). Agitation modules: Flexible means to accelerate automated freeze substitution. Journal of Histochemistry and Cytochemistry. SAGE Publications. https://doi.org/10.1369/0022155418786698","ieee":"S. Reipert et al., “Agitation modules: Flexible means to accelerate automated freeze substitution,” Journal of Histochemistry and Cytochemistry, vol. 66, no. 12. SAGE Publications, pp. 903–921, 2018."},"article_processing_charge":"No","abstract":[{"text":"For ultrafast fixation of biological samples to avoid artifacts, high-pressure freezing (HPF) followed by freeze substitution (FS) is preferred over chemical fixation at room temperature. After HPF, samples are maintained at low temperature during dehydration and fixation, while avoiding damaging recrystallization. This is a notoriously slow process. McDonald and Webb demonstrated, in 2011, that sample agitation during FS dramatically reduces the necessary time. Then, in 2015, we (H.G. and S.R.) introduced an agitation module into the cryochamber of an automated FS unit and demonstrated that the preparation of algae could be shortened from days to a couple of hours. We argued that variability in the processing, reproducibility, and safety issues are better addressed using automated FS units. For dissemination, we started low-cost manufacturing of agitation modules for two of the most widely used FS units, the Automatic Freeze Substitution Systems, AFS(1) and AFS2, from Leica Microsystems, using three dimensional (3D)-printing of the major components. To test them, several labs independently used the modules on a wide variety of specimens that had previously been processed by manual agitation, or without agitation. We demonstrate that automated processing with sample agitation saves time, increases flexibility with respect to sample requirements and protocols, and produces data of at least as good quality as other approaches.","lang":"eng"}],"page":"903-921","scopus_import":"1","pmid":1,"external_id":{"isi":["000452277700005"],"pmid":["29969056"]},"date_created":"2018-12-11T11:44:57Z","article_type":"original","oa":1,"oa_version":"Published Version","quality_controlled":"1","title":"Agitation modules: Flexible means to accelerate automated freeze substitution","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"SAGE Publications","issue":"12","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1369/0022155418786698"}]},{"month":"05","date_published":"2018-05-09T00:00:00Z","_id":"5686","department":[{"_id":"E-Lib"}],"article_processing_charge":"No","file":[{"file_name":"2018_WorkingPaper_Danowski.pdf","date_created":"2019-01-22T09:06:51Z","content_type":"application/pdf","file_id":"5872","date_updated":"2020-07-14T12:47:10Z","checksum":"6cb95f8772491d155ce77c6160655fff","creator":"dernst","file_size":202798,"access_level":"open_access","relation":"main_file"}],"page":"5","has_accepted_license":"1","scopus_import":1,"related_material":{"record":[{"status":"public","id":"6657","relation":"later_version"}]},"day":"09","date_updated":"2023-10-17T11:33:57Z","year":"2018","author":[{"full_name":"Danowski, Patrick","orcid":"0000-0002-6026-4409","id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","first_name":"Patrick","last_name":"Danowski"}],"file_date_updated":"2020-07-14T12:47:10Z","date_created":"2018-12-17T10:28:26Z","oa":1,"ddc":["020"],"language":[{"iso":"eng"}],"doi":"10.5281/zenodo.1244154","publication_status":"published","oa_version":"Published Version","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","type":"working_paper","title":"An Austrian proposal for the Classification of Open Access Tuples (COAT) - Distinguish different Open Access types beyond colors","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"P. Danowski, An Austrian proposal for the Classification of Open Access Tuples (COAT) - Distinguish different Open Access types beyond colors. 2018.","short":"P. Danowski, An Austrian Proposal for the Classification of Open Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors, 2018.","apa":"Danowski, P. (2018). An Austrian proposal for the Classification of Open Access Tuples (COAT) - Distinguish different Open Access types beyond colors. https://doi.org/10.5281/zenodo.1244154","ista":"Danowski P. 2018. An Austrian proposal for the Classification of Open Access Tuples (COAT) - Distinguish different Open Access types beyond colors, 5p.","ama":"Danowski P. An Austrian Proposal for the Classification of Open Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors.; 2018. doi:10.5281/zenodo.1244154","chicago":"Danowski, Patrick. An Austrian Proposal for the Classification of Open Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors, 2018. https://doi.org/10.5281/zenodo.1244154.","mla":"Danowski, Patrick. An Austrian Proposal for the Classification of Open Access Tuples (COAT) - Distinguish Different Open Access Types beyond Colors. 2018, doi:10.5281/zenodo.1244154."}},{"type":"research_data","title":"Emerald Austrian Publications 2013-2017","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Institute of Science and Technology Austria","status":"public","oa_version":"Submitted Version","tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)","short":"CC0 (1.0)"},"citation":{"ista":"Villányi M. 2018. Emerald Austrian Publications 2013-2017, Institute of Science and Technology Austria, 10.15479/AT:ISTA:89.","ama":"Villányi M. Emerald Austrian Publications 2013-2017. 2018. doi:10.15479/AT:ISTA:89","mla":"Villányi, Márton. Emerald Austrian Publications 2013-2017. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:89.","chicago":"Villányi, Márton. “Emerald Austrian Publications 2013-2017.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:89.","ieee":"M. Villányi, “Emerald Austrian Publications 2013-2017.” Institute of Science and Technology Austria, 2018.","short":"M. Villányi, (2018).","apa":"Villányi, M. (2018). Emerald Austrian Publications 2013-2017. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:89"},"date_created":"2018-12-12T12:31:37Z","keyword":["Publication analysis","Bibliography","Open Access"],"file_date_updated":"2020-07-14T12:47:06Z","author":[{"last_name":"Villányi","first_name":"Márton","id":"3FFCCD3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8126-0426","full_name":"Villányi, Márton"}],"doi":"10.15479/AT:ISTA:89","ddc":["020"],"oa":1,"day":"16","license":"https://creativecommons.org/publicdomain/zero/1.0/","related_material":{"record":[{"relation":"part_of_dissertation","id":"278","status":"public"}]},"year":"2018","date_updated":"2024-02-21T13:41:32Z","date_published":"2018-01-16T00:00:00Z","month":"01","datarep_id":"89","has_accepted_license":"1","abstract":[{"lang":"ger","text":"Data on Austrian open access publication output at Emerald from 2013-2017 including data analysis."}],"file":[{"file_name":"IST-2018-89-v1+1_Emerald_Austrian_Publications_2013-2017.zip","date_created":"2018-12-12T13:02:39Z","file_id":"5604","content_type":"application/zip","access_level":"open_access","relation":"main_file","file_size":222011,"checksum":"786b599abfae6c355dee87835f414549","creator":"system","date_updated":"2020-07-14T12:47:06Z"}],"department":[{"_id":"E-Lib"}],"article_processing_charge":"No","_id":"5577"},{"date_published":"2018-01-16T00:00:00Z","month":"01","datarep_id":"90","abstract":[{"text":"Data on Austrian open access publication output at IOP from 2012-2015 including data analysis.","lang":"ger"}],"has_accepted_license":"1","file":[{"date_created":"2018-12-12T13:03:06Z","file_name":"IST-2018-90-v1+1_IOP_Austrian_Publications_2012-2015.zip","file_id":"5624","content_type":"application/zip","relation":"main_file","access_level":"open_access","file_size":237067,"creator":"system","checksum":"a4f1bf041ccd4c35912e2d595b0c2883","date_updated":"2020-07-14T12:47:06Z"}],"article_processing_charge":"No","department":[{"_id":"E-Lib"}],"_id":"5578","day":"16","related_material":{"record":[{"status":"public","id":"278","relation":"part_of_dissertation"}]},"year":"2018","date_updated":"2024-02-21T13:42:36Z","date_created":"2018-12-12T12:31:38Z","keyword":["Publication analysis","Bibliography","Open Access"],"file_date_updated":"2020-07-14T12:47:06Z","author":[{"id":"3FFCCD3A-F248-11E8-B48F-1D18A9856A87","first_name":"Márton","last_name":"Villányi","full_name":"Villányi, Márton","orcid":"0000-0001-8126-0426"}],"doi":"10.15479/AT:ISTA:90","ddc":["020"],"oa":1,"title":"IOP Austrian Publications 2012-2015","type":"research_data","status":"public","publisher":"Institute of Science and Technology Austria","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Submitted Version","citation":{"apa":"Villányi, M. (2018). IOP Austrian Publications 2012-2015. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:90","short":"M. Villányi, (2018).","ieee":"M. Villányi, “IOP Austrian Publications 2012-2015.” Institute of Science and Technology Austria, 2018.","chicago":"Villányi, Márton. “IOP Austrian Publications 2012-2015.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:90.","mla":"Villányi, Márton. IOP Austrian Publications 2012-2015. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:90.","ama":"Villányi M. IOP Austrian Publications 2012-2015. 2018. doi:10.15479/AT:ISTA:90","ista":"Villányi M. 2018. IOP Austrian Publications 2012-2015, Institute of Science and Technology Austria, 10.15479/AT:ISTA:90."},"tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)","short":"CC0 (1.0)"}},{"tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)","short":"CC0 (1.0)"},"citation":{"ieee":"M. Villányi, “Data Check IOP Scopus vs. Publisher.” Institute of Science and Technology Austria, 2018.","apa":"Villányi, M. (2018). Data Check IOP Scopus vs. Publisher. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:86","short":"M. Villányi, (2018).","ista":"Villányi M. 2018. Data Check IOP Scopus vs. Publisher, Institute of Science and Technology Austria, 10.15479/AT:ISTA:86.","ama":"Villányi M. Data Check IOP Scopus vs. Publisher. 2018. doi:10.15479/AT:ISTA:86","mla":"Villányi, Márton. Data Check IOP Scopus vs. Publisher. Institute of Science and Technology Austria, 2018, doi:10.15479/AT:ISTA:86.","chicago":"Villányi, Márton. “Data Check IOP Scopus vs. Publisher.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:86."},"oa_version":"Submitted Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","publisher":"Institute of Science and Technology Austria","title":"Data Check IOP Scopus vs. Publisher","type":"research_data","oa":1,"ddc":["020"],"doi":"10.15479/AT:ISTA:86","author":[{"last_name":"Villányi","first_name":"Márton","id":"3FFCCD3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8126-0426","full_name":"Villányi, Márton"}],"file_date_updated":"2020-07-14T12:47:05Z","keyword":["Publication analysis","Bibliography","Open Access"],"date_created":"2018-12-12T12:31:37Z","date_updated":"2024-02-21T13:42:21Z","year":"2018","related_material":{"record":[{"status":"public","id":"278","relation":"part_of_dissertation"}]},"day":"16","_id":"5574","department":[{"_id":"E-Lib"}],"article_processing_charge":"No","abstract":[{"lang":"ger","text":"Comparison of Scopus' and publisher's data on Austrian publication output at IOP. "}],"has_accepted_license":"1","file":[{"date_created":"2018-12-12T13:05:14Z","file_name":"IST-2018-86-v1+1_Data_Check_IOP_Scopus_vs._Publisher.zip","file_id":"5642","content_type":"application/zip","date_updated":"2020-07-14T12:47:05Z","checksum":"c7a61147bd15cb4ae45878d270628c06","creator":"system","file_size":12283857,"access_level":"open_access","relation":"main_file"}],"datarep_id":"86","month":"01","date_published":"2018-01-16T00:00:00Z"},{"department":[{"_id":"E-Lib"}],"_id":"278","page":"94","abstract":[{"lang":"eng","text":"Consortial subscription contracts regulate the digital access to publications between publishers and scientific libraries. However, since a couple of years the tendency towards a freely accessible publishing (Open Access) intensifies. As a consequence of this trend the contractual relationship between licensor and licensee is gradually changing as well: More and more contracts exercise influence on open access publishing. The present study attempts to compare Austrian examples of consortial licence contracts, which include components of open access. It describes the difference between pure subscription contracts and differing innovative deals including open access components. Thereby it becomes obvious that for the evaluation of this licence contracts new methods are needed. An essential new element of such analyses is the evaluation of the open access publication numbers. So this study tries to carry out such publication analyses for Austrian open access deals focusing on quantitative questions: How does the number of publications evolve? How does the open access share change? Publications reports of the publishers and database queries from Scopus form the data basis. The analysis of the data points out that differing approaches of contracts result in highly divergent results: Particular deals can prioritize a saving in costs or else the increase of the open access rate. It is to be assumed that within the following years further numerous open access deals will be negotiated. The finding of this study shall provide guidance."}],"date_published":"2018-04-06T00:00:00Z","month":"04","date_updated":"2024-02-21T13:44:07Z","year":"2018","related_material":{"record":[{"id":"5577","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"5574"},{"id":"5578","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"5579"},{"status":"public","relation":"dissertation_contains","id":"5576"},{"relation":"dissertation_contains","id":"5575","status":"public"},{"relation":"dissertation_contains","id":"5582","status":"public"},{"relation":"dissertation_contains","id":"5581","status":"public"},{"id":"5580","relation":"dissertation_contains","status":"public"}]},"day":"06","language":[{"iso":"ger"}],"oa":1,"author":[{"id":"3FFCCD3A-F248-11E8-B48F-1D18A9856A87","first_name":"Márton","last_name":"Villányi","full_name":"Villányi, Márton","orcid":"0000-0001-8126-0426"}],"date_created":"2018-12-11T11:45:34Z","publist_id":"7624","citation":{"ieee":"M. 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F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687, and J.R. was funded by an EMBO long-term fellowship (ALTF 1396-2014).","doi":"10.1038/s41590-018-0109-z","publication":"Nature Immunology","publication_status":"published","status":"public","type":"journal_article","volume":19,"citation":{"apa":"Hons, M., Kopf, A., Hauschild, R., Leithner, A. F., Gärtner, F. R., Abe, J., … Sixt, M. K. (2018). Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. Nature Publishing Group. https://doi.org/10.1038/s41590-018-0109-z","short":"M. Hons, A. Kopf, R. Hauschild, A.F. Leithner, F.R. Gärtner, J. Abe, J. Renkawitz, J. Stein, M.K. Sixt, Nature Immunology 19 (2018) 606–616.","ieee":"M. Hons et al., “Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells,” Nature Immunology, vol. 19, no. 6. Nature Publishing Group, pp. 606–616, 2018.","chicago":"Hons, Miroslav, Aglaja Kopf, Robert Hauschild, Alexander F Leithner, Florian R Gärtner, Jun Abe, Jörg Renkawitz, Jens Stein, and Michael K Sixt. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” Nature Immunology. Nature Publishing Group, 2018. https://doi.org/10.1038/s41590-018-0109-z.","mla":"Hons, Miroslav, et al. “Chemokines and Integrins Independently Tune Actin Flow and Substrate Friction during Intranodal Migration of T Cells.” Nature Immunology, vol. 19, no. 6, Nature Publishing Group, 2018, pp. 606–16, doi:10.1038/s41590-018-0109-z.","ama":"Hons M, Kopf A, Hauschild R, et al. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. 2018;19(6):606-616. doi:10.1038/s41590-018-0109-z","ista":"Hons M, Kopf A, Hauschild R, Leithner AF, Gärtner FR, Abe J, Renkawitz J, Stein J, Sixt MK. 2018. Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells. Nature Immunology. 19(6), 606–616."},"project":[{"grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Cellular navigation along spatial gradients"},{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020"},{"grant_number":"ALTF 1396-2014","_id":"25A48D24-B435-11E9-9278-68D0E5697425","name":"Molecular and system level view of immune cell migration"},{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556"}],"article_processing_charge":"No","abstract":[{"lang":"eng","text":"Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together with analysis of cytoskeletal dynamics in vitro to deconstruct the basic mechanisms of T cell migration within lymphatic organs. We show that the contributions of the integrin LFA-1 and the chemokine receptor CCR7 are complementary rather than positioned in a linear pathway, as they are during leukocyte extravasation from the blood vasculature. Our data demonstrate that CCR7 controls cortical actin flows, whereas integrins mediate substrate friction that is sufficient to drive locomotion in the absence of considerable surface adhesions and plasma membrane flux."}],"page":"606 - 616","scopus_import":"1","external_id":{"pmid":["29777221"],"isi":["000433041500026"]},"pmid":1,"ec_funded":1,"date_created":"2018-12-11T11:44:10Z","oa":1,"quality_controlled":"1","oa_version":"Published Version","acknowledged_ssus":[{"_id":"SSU"}],"publisher":"Nature Publishing Group","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Chemokines and integrins independently tune actin flow and substrate friction during intranodal migration of T cells","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pubmed/29777221"}],"issue":"6"},{"related_material":{"record":[{"relation":"dissertation_contains","id":"10083","status":"public"}]},"day":"05","date_updated":"2024-03-18T23:30:45Z","year":"2018","month":"01","date_published":"2018-01-05T00:00:00Z","_id":"442","department":[{"_id":"JiFr"},{"_id":"Bio"}],"publication_status":"published","pubrep_id":"970","status":"public","type":"journal_article","volume":8,"citation":{"apa":"Li, L., Krens, G., Fendrych, M., & Friml, J. (2018). Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-Protocol. Bio-protocol. https://doi.org/10.21769/BioProtoc.2685","short":"L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).","ieee":"L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” Bio-protocol, vol. 8, no. 1. Bio-protocol, 2018.","mla":"Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” Bio-Protocol, vol. 8, no. 1, Bio-protocol, 2018, doi:10.21769/BioProtoc.2685.","chicago":"Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” Bio-Protocol. Bio-protocol, 2018. https://doi.org/10.21769/BioProtoc.2685.","ista":"Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol. 8(1).","ama":"Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol. 2018;8(1). doi:10.21769/BioProtoc.2685"},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"author":[{"first_name":"Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li","full_name":"Li, Lanxin","orcid":"0000-0002-5607-272X"},{"last_name":"Krens","first_name":"Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel"},{"last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","first_name":"Matyas","orcid":"0000-0002-9767-8699","full_name":"Fendrych, Matyas"},{"last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"file_date_updated":"2020-07-14T12:46:29Z","publist_id":"7381","publication_identifier":{"eissn":["2331-8325"]},"language":[{"iso":"eng"}],"intvolume":" 8","acknowledgement":"This protocol was adapted from Fendrych et al., 2016. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian Science Fund (FWF) [M 2128-B21]. ","doi":"10.21769/BioProtoc.2685","publication":"Bio-protocol","project":[{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020"}],"article_processing_charge":"No","has_accepted_license":"1","abstract":[{"lang":"eng","text":"The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH."}],"file":[{"creator":"system","checksum":"6644ba698206eda32b0abf09128e63e3","date_updated":"2020-07-14T12:46:29Z","relation":"main_file","access_level":"open_access","file_size":11352389,"file_name":"IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf","date_created":"2018-12-12T10:17:43Z","file_id":"5299","content_type":"application/pdf"}],"quality_controlled":"1","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Bio-protocol","title":"Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls","issue":"1","ec_funded":1,"date_created":"2018-12-11T11:46:30Z","oa":1,"ddc":["576","581"],"article_type":"original"},{"day":"26","date_updated":"2020-07-14T23:05:03Z","year":"2017","date_published":"2017-06-26T00:00:00Z","month":"06","department":[{"_id":"E-Lib"}],"_id":"5450","abstract":[{"lang":"eng","text":"In this report the implementation of the institutional data repository IST DataRep at IST Austria will be covered: Starting with the research phase when requirements for a repository were established, the procedure of choosing a repository-software and its customization based on the results of user-testings will be discussed. Followed by reflections on the marketing strategies in regard of impact, and at the end sharing some experiences of one year operating IST DataRep."}],"publication_date":"2017-06-26","file":[{"date_updated":"2020-07-14T12:46:59Z","checksum":"6321792dcfa82bf490f17615a9b22355","creator":"system","file_size":3460985,"relation":"main_file","access_level":"open_access","date_created":"2018-12-12T11:53:22Z","file_name":"IST-2017-724-v1+1_DataRep_Project_Report_2017.pdf","content_type":"application/pdf","file_id":"5483"}],"pubrep_id":"724","title":"Implementing the institutional data repository IST DataRep","type":"report","publisher":"IST Austria","status":"public","citation":{"ieee":"B. Petritsch, Implementing the institutional data repository IST DataRep. IST Austria, 2017.","apa":"Petritsch, B. (2017). Implementing the institutional data repository IST DataRep. IST Austria.","short":"B. Petritsch, Implementing the Institutional Data Repository IST DataRep, IST Austria, 2017.","ista":"Petritsch B. 2017. Implementing the institutional data repository IST DataRep, IST Austria,p.","ama":"Petritsch B. Implementing the Institutional Data Repository IST DataRep. IST Austria; 2017.","chicago":"Petritsch, Barbara. Implementing the Institutional Data Repository IST DataRep. IST Austria, 2017.","mla":"Petritsch, Barbara. Implementing the Institutional Data Repository IST DataRep. IST Austria, 2017."},"main_file_link":[{"url":"https://repository.ist.ac.at/id/eprint/724.","open_access":"1"}],"file_date_updated":"2020-07-14T12:46:59Z","author":[{"full_name":"Barbara Petritsch","orcid":"0000-0003-2724-4614","first_name":"Barbara","id":"406048EC-F248-11E8-B48F-1D18A9856A87","last_name":"Petritsch"}],"date_created":"2018-12-12T11:39:24Z","extern":0,"oa":1},{"scopus_import":1,"abstract":[{"text":"Background: Standards have become available to share semantically encoded vital parameters from medical devices, as required for example by personal healthcare records. Standardised sharing of biosignal data largely remains open. Objectives: The goal of this work is to explore available biosignal file format and data exchange standards and profiles, and to conceptualise end-To-end solutions. Methods: The authors reviewed and discussed available biosignal file format standards with other members of international standards development organisations (SDOs). Results: A raw concept for standards based acquisition, storage, archiving and sharing of biosignals was developed. The GDF format may serve for storing biosignals. Signals can then be shared using FHIR resources and may be stored on FHIR servers or in DICOM archives, with DICOM waveforms as one possible format. Conclusion: Currently a group of international SDOs (e.g. HL7, IHE, DICOM, IEEE) is engaged in intensive discussions. This discussion extends existing work that already was adopted by large implementer communities. The concept presented here only reports the current status of the discussion in Austria. The discussion will continue internationally, with results to be expected over the coming years.","lang":"eng"}],"has_accepted_license":"1","page":"356 - 362","file":[{"date_updated":"2020-07-14T12:47:27Z","creator":"system","checksum":"1254dcc5b04a996d97fad9a726b42727","file_size":443635,"access_level":"open_access","relation":"main_file","file_id":"4913","content_type":"application/pdf","file_name":"IST-2017-906-v1+1_SHTI236-0356.pdf","date_created":"2018-12-12T10:11:56Z"}],"title":"Biosignals standards and FHIR: The way to go","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"IOS Press","oa_version":"Published Version","quality_controlled":"1","ddc":["005"],"oa":1,"date_created":"2018-12-11T11:47:36Z","year":"2017","date_updated":"2021-01-12T08:06:59Z","day":"01","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"_id":"630","date_published":"2017-01-01T00:00:00Z","month":"01","alternative_title":["Studies in Health Technology and Informatics"],"tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)"},"citation":{"apa":"Sauermann, S., David, V., Schlögl, A., Egelkraut, R., Frohner, M., Pohn, B., … Mense, A. (2017). Biosignals standards and FHIR: The way to go (Vol. 236, pp. 356–362). Presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria: IOS Press. https://doi.org/10.3233/978-1-61499-759-7-356","short":"S. Sauermann, V. David, A. Schlögl, R. Egelkraut, M. Frohner, B. Pohn, P. Urbauer, A. Mense, in:, IOS Press, 2017, pp. 356–362.","ieee":"S. Sauermann et al., “Biosignals standards and FHIR: The way to go,” presented at the eHealth: Health Informatics Meets eHealth, Vienna, Austria, 2017, vol. 236, pp. 356–362.","chicago":"Sauermann, Stefan, Veronika David, Alois Schlögl, Reinhard Egelkraut, Matthias Frohner, Birgit Pohn, Philipp Urbauer, and Alexander Mense. “Biosignals Standards and FHIR: The Way to Go,” 236:356–62. IOS Press, 2017. https://doi.org/10.3233/978-1-61499-759-7-356.","mla":"Sauermann, Stefan, et al. Biosignals Standards and FHIR: The Way to Go. Vol. 236, IOS Press, 2017, pp. 356–62, doi:10.3233/978-1-61499-759-7-356.","ista":"Sauermann S, David V, Schlögl A, Egelkraut R, Frohner M, Pohn B, Urbauer P, Mense A. 2017. Biosignals standards and FHIR: The way to go. eHealth: Health Informatics Meets eHealth, Studies in Health Technology and Informatics, vol. 236, 356–362.","ama":"Sauermann S, David V, Schlögl A, et al. Biosignals standards and FHIR: The way to go. In: Vol 236. IOS Press; 2017:356-362. doi:10.3233/978-1-61499-759-7-356"},"volume":236,"type":"conference","status":"public","pubrep_id":"906","publication_status":"published","conference":{"location":"Vienna, Austria","start_date":"2017-05-23","name":"eHealth: Health Informatics Meets eHealth","end_date":"2017-05-24"},"doi":"10.3233/978-1-61499-759-7-356","intvolume":" 236","language":[{"iso":"eng"}],"publication_identifier":{"isbn":["978-161499758-0"]},"publist_id":"7164","file_date_updated":"2020-07-14T12:47:27Z","author":[{"last_name":"Sauermann","first_name":"Stefan","full_name":"Sauermann, Stefan"},{"last_name":"David","first_name":"Veronika","full_name":"David, Veronika"},{"id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"first_name":"Reinhard","last_name":"Egelkraut","full_name":"Egelkraut, Reinhard"},{"last_name":"Frohner","first_name":"Matthias","full_name":"Frohner, Matthias"},{"full_name":"Pohn, Birgit","last_name":"Pohn","first_name":"Birgit"},{"full_name":"Urbauer, Philipp","first_name":"Philipp","last_name":"Urbauer"},{"last_name":"Mense","first_name":"Alexander","full_name":"Mense, Alexander"}]},{"scopus_import":1,"abstract":[{"lang":"eng","text":"Trafficking cells frequently transmigrate through epithelial and endothelial monolayers. How monolayers cooperate with the penetrating cells to support their transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic capillaries as a model system for transendothelial migration. We find that the chemokine CCL21, which is the decisive guidance cue for intravasation, mainly localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes extracellularly enriched at the sites of endothelial cell-cell junctions. When we reconstitute the transmigration process in vitro, we find that secretion of CCL21-positive vesicles is triggered by a DC contact-induced calcium signal, and selective calcium chelation in lymphatic endothelium attenuates transmigration. Altogether, our data demonstrate a chemokine-mediated feedback between DCs and lymphatic endothelium, which facilitates transendothelial migration."}],"page":"902 - 909","file":[{"access_level":"open_access","relation":"main_file","file_size":2248814,"checksum":"8fdddaab1f1d76a6ec9ca94dcb6b07a2","creator":"system","date_updated":"2020-07-14T12:47:38Z","file_id":"5109","content_type":"application/pdf","date_created":"2018-12-12T10:14:54Z","file_name":"IST-2017-900-v1+1_1-s2.0-S2211124717305211-main.pdf"}],"has_accepted_license":"1","project":[{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7"},{"_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12","call_identifier":"FWF","name":"Cytoskeletal force generation and transduction of leukocytes (FWF)"}],"article_processing_charge":"Yes","issue":"5","publisher":"Cell Press","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","title":"Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia","quality_controlled":"1","oa_version":"Published Version","oa":1,"ddc":["570"],"ec_funded":1,"date_created":"2018-12-11T11:47:50Z","year":"2017","date_updated":"2023-02-23T12:50:09Z","day":"02","_id":"672","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"EM-Fac"}],"month":"05","date_published":"2017-05-02T00:00:00Z","citation":{"chicago":"Vaahtomeri, Kari, Markus Brown, Robert Hauschild, Ingrid de Vries, Alexander F Leithner, Matthias Mehling, Walter Kaufmann, and Michael K Sixt. “Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” Cell Reports. Cell Press, 2017. https://doi.org/10.1016/j.celrep.2017.04.027.","mla":"Vaahtomeri, Kari, et al. “Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia.” Cell Reports, vol. 19, no. 5, Cell Press, 2017, pp. 902–09, doi:10.1016/j.celrep.2017.04.027.","ama":"Vaahtomeri K, Brown M, Hauschild R, et al. Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. 2017;19(5):902-909. doi:10.1016/j.celrep.2017.04.027","ista":"Vaahtomeri K, Brown M, Hauschild R, de Vries I, Leithner AF, Mehling M, Kaufmann W, Sixt MK. 2017. Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. 19(5), 902–909.","short":"K. Vaahtomeri, M. Brown, R. Hauschild, I. de Vries, A.F. Leithner, M. Mehling, W. Kaufmann, M.K. Sixt, Cell Reports 19 (2017) 902–909.","apa":"Vaahtomeri, K., Brown, M., Hauschild, R., de Vries, I., Leithner, A. F., Mehling, M., … Sixt, M. K. (2017). Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2017.04.027","ieee":"K. Vaahtomeri et al., “Locally triggered release of the chemokine CCL21 promotes dendritic cell transmigration across lymphatic endothelia,” Cell Reports, vol. 19, no. 5. Cell Press, pp. 902–909, 2017."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"status":"public","type":"journal_article","volume":19,"publication_status":"published","pubrep_id":"900","doi":"10.1016/j.celrep.2017.04.027","publication":"Cell Reports","publication_identifier":{"issn":["22111247"]},"intvolume":" 19","language":[{"iso":"eng"}],"publist_id":"7052","author":[{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","first_name":"Kari","last_name":"Vaahtomeri","full_name":"Vaahtomeri, Kari","orcid":"0000-0001-7829-3518"},{"last_name":"Brown","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","first_name":"Markus","full_name":"Brown, Markus"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"De Vries","full_name":"De Vries, Ingrid"},{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F","last_name":"Leithner","full_name":"Leithner, Alexander F"},{"last_name":"Mehling","first_name":"Matthias","id":"3C23B994-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8599-1226","full_name":"Mehling, Matthias"},{"full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann"},{"orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"file_date_updated":"2020-07-14T12:47:38Z"},{"page":"1314 - 1325","abstract":[{"lang":"eng","text":"Navigation of cells along gradients of guidance cues is a determining step in many developmental and immunological processes. Gradients can either be soluble or immobilized to tissues as demonstrated for the haptotactic migration of dendritic cells (DCs) toward higher concentrations of immobilized chemokine CCL21. To elucidate how gradient characteristics govern cellular response patterns, we here introduce an in vitro system allowing to track migratory responses of DCs to precisely controlled immobilized gradients of CCL21. We find that haptotactic sensing depends on the absolute CCL21 concentration and local steepness of the gradient, consistent with a scenario where DC directionality is governed by the signal-to-noise ratio of CCL21 binding to the receptor CCR7. We find that the conditions for optimal DC guidance are perfectly provided by the CCL21 gradients we measure in vivo. Furthermore, we find that CCR7 signal termination by the G-protein-coupled receptor kinase 6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient sensing in vitro and confirm those observations in vivo. These findings suggest that stable, tissue-bound CCL21 gradients as sustainable “roads” ensure optimal guidance in vivo."}],"project":[{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"name":"Cytoskeletal force generation and transduction of leukocytes (FWF)","call_identifier":"FWF","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12"}],"scopus_import":1,"ec_funded":1,"date_created":"2018-12-11T11:47:51Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publisher":"Cell Press","title":"Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6","oa_version":"None","quality_controlled":"1","issue":"9","month":"05","date_published":"2017-05-09T00:00:00Z","_id":"674","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"day":"09","year":"2017","date_updated":"2023-02-23T12:50:44Z","publist_id":"7050","author":[{"first_name":"Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","last_name":"Schwarz","full_name":"Schwarz, Jan"},{"last_name":"Bierbaum","id":"3FD04378-F248-11E8-B48F-1D18A9856A87","first_name":"Veronika","full_name":"Bierbaum, Veronika"},{"orcid":"0000-0001-7829-3518","full_name":"Vaahtomeri, Kari","last_name":"Vaahtomeri","first_name":"Kari","id":"368EE576-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Markus","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","last_name":"Brown","full_name":"Brown, Markus"},{"full_name":"De Vries, Ingrid","last_name":"De Vries","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Leithner, Alexander F","first_name":"Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","last_name":"Leithner"},{"orcid":"0000-0003-0666-8928","full_name":"Reversat, Anne","last_name":"Reversat","id":"35B76592-F248-11E8-B48F-1D18A9856A87","first_name":"Anne"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin"},{"last_name":"Tarrant","first_name":"Teresa","full_name":"Tarrant, Teresa"},{"full_name":"Bollenbach, Tobias","orcid":"0000-0003-4398-476X","first_name":"Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","last_name":"Bollenbach"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"doi":"10.1016/j.cub.2017.04.004","publication":"Current Biology","publication_identifier":{"issn":["09609822"]},"language":[{"iso":"eng"}],"intvolume":" 27","status":"public","type":"journal_article","volume":27,"publication_status":"published","citation":{"mla":"Schwarz, Jan, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” Current Biology, vol. 27, no. 9, Cell Press, 2017, pp. 1314–25, doi:10.1016/j.cub.2017.04.004.","chicago":"Schwarz, Jan, Veronika Bierbaum, Kari Vaahtomeri, Robert Hauschild, Markus Brown, Ingrid de Vries, Alexander F Leithner, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” Current Biology. Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.04.004.","ista":"Schwarz J, Bierbaum V, Vaahtomeri K, Hauschild R, Brown M, de Vries I, Leithner AF, Reversat A, Merrin J, Tarrant T, Bollenbach MT, Sixt MK. 2017. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. 27(9), 1314–1325.","ama":"Schwarz J, Bierbaum V, Vaahtomeri K, et al. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. 2017;27(9):1314-1325. doi:10.1016/j.cub.2017.04.004","apa":"Schwarz, J., Bierbaum, V., Vaahtomeri, K., Hauschild, R., Brown, M., de Vries, I., … Sixt, M. K. (2017). Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2017.04.004","short":"J. Schwarz, V. Bierbaum, K. Vaahtomeri, R. Hauschild, M. Brown, I. de Vries, A.F. Leithner, A. Reversat, J. Merrin, T. Tarrant, M.T. Bollenbach, M.K. Sixt, Current Biology 27 (2017) 1314–1325.","ieee":"J. Schwarz et al., “Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6,” Current Biology, vol. 27, no. 9. Cell Press, pp. 1314–1325, 2017."}},{"date_published":"2017-06-27T00:00:00Z","month":"06","department":[{"_id":"EM-Fac"},{"_id":"RySh"}],"_id":"693","day":"27","date_updated":"2023-02-23T12:54:57Z","year":"2017","file_date_updated":"2020-07-14T12:47:44Z","author":[{"full_name":"Miki, Takafumi","last_name":"Miki","first_name":"Takafumi"},{"first_name":"Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","last_name":"Kaufmann","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315"},{"first_name":"Gerardo","last_name":"Malagon","full_name":"Malagon, Gerardo"},{"first_name":"Laura","last_name":"Gomez","full_name":"Gomez, Laura"},{"first_name":"Katsuhiko","last_name":"Tabuchi","full_name":"Tabuchi, Katsuhiko"},{"last_name":"Watanabe","first_name":"Masahiko","full_name":"Watanabe, Masahiko"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Marty, Alain","first_name":"Alain","last_name":"Marty"}],"publist_id":"7013","language":[{"iso":"eng"}],"intvolume":" 114","publication_identifier":{"issn":["00278424"]},"publication":"PNAS","doi":"10.1073/pnas.1704470114","publication_status":"published","volume":114,"type":"journal_article","status":"public","citation":{"ama":"Miki T, Kaufmann W, Malagon G, et al. Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses. PNAS. 2017;114(26):E5246-E5255. doi:10.1073/pnas.1704470114","ista":"Miki T, Kaufmann W, Malagon G, Gomez L, Tabuchi K, Watanabe M, Shigemoto R, Marty A. 2017. Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses. PNAS. 114(26), E5246–E5255.","chicago":"Miki, Takafumi, Walter Kaufmann, Gerardo Malagon, Laura Gomez, Katsuhiko Tabuchi, Masahiko Watanabe, Ryuichi Shigemoto, and Alain Marty. “Numbers of Presynaptic Ca2+ Channel Clusters Match Those of Functionally Defined Vesicular Docking Sites in Single Central Synapses.” PNAS. National Academy of Sciences, 2017. https://doi.org/10.1073/pnas.1704470114.","mla":"Miki, Takafumi, et al. “Numbers of Presynaptic Ca2+ Channel Clusters Match Those of Functionally Defined Vesicular Docking Sites in Single Central Synapses.” PNAS, vol. 114, no. 26, National Academy of Sciences, 2017, pp. E5246–55, doi:10.1073/pnas.1704470114.","ieee":"T. Miki et al., “Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses,” PNAS, vol. 114, no. 26. National Academy of Sciences, pp. E5246–E5255, 2017.","short":"T. Miki, W. Kaufmann, G. Malagon, L. Gomez, K. Tabuchi, M. Watanabe, R. Shigemoto, A. Marty, PNAS 114 (2017) E5246–E5255.","apa":"Miki, T., Kaufmann, W., Malagon, G., Gomez, L., Tabuchi, K., Watanabe, M., … Marty, A. (2017). Numbers of presynaptic Ca2+ channel clusters match those of functionally defined vesicular docking sites in single central synapses. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1704470114"},"article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","page":"E5246 - E5255","abstract":[{"lang":"eng","text":"Many central synapses contain a single presynaptic active zone and a single postsynaptic density. Vesicular release statistics at such “simple synapses” indicate that they contain a small complement of docking sites where vesicles repetitively dock and fuse. In this work, we investigate functional and morphological aspects of docking sites at simple synapses made between cerebellar parallel fibers and molecular layer interneurons. Using immunogold labeling of SDS-treated freeze-fracture replicas, we find that Cav2.1 channels form several clusters per active zone with about nine channels per cluster. The mean value and range of intersynaptic variation are similar for Cav2.1 cluster numbers and for functional estimates of docking-site numbers obtained from the maximum numbers of released vesicles per action potential. Both numbers grow in relation with synaptic size and decrease by a similar extent with age between 2 wk and 4 wk postnatal. Thus, the mean docking-site numbers were 3.15 at 2 wk (range: 1–10) and 2.03 at 4 wk (range: 1–4), whereas the mean numbers of Cav2.1 clusters were 2.84 at 2 wk (range: 1–8) and 2.37 at 4 wk (range: 1–5). These changes were accompanied by decreases of miniature current amplitude (from 93 pA to 56 pA), active-zone surface area (from 0.0427 μm2 to 0.0234 μm2), and initial success rate (from 0.609 to 0.353), indicating a tightening of synaptic transmission with development. Altogether, these results suggest a close correspondence between the number of functionally defined vesicular docking sites and that of clusters of voltage-gated calcium channels. 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More than 1000 papers by Austrian authors were published open access at Springer in the first year alone. The working group "Springer Compact Evaluierung" made the data for these articles available via the platform OpenAPC and would like to use this opportunity to give a short account of what this publishing agreement actually entails and the working group intends to do."}],"has_accepted_license":"1","file":[{"file_name":"2017_VOEB_Andrae.pdf","date_created":"2019-01-18T13:39:26Z","file_id":"5851","content_type":"application/pdf","creator":"dernst","checksum":"558c18bcf5580d87dd371ec626d52075","date_updated":"2020-07-14T12:48:09Z","access_level":"open_access","relation":"main_file","file_size":125065}],"scopus_import":1,"publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare","doi":"10.31263/voebm.v70i2.1898","language":[{"iso":"eng"}],"intvolume":" 70","publication_identifier":{"issn":["10222588"]},"publist_id":"6843","file_date_updated":"2020-07-14T12:48:09Z","author":[{"full_name":"Andrae, Magdalena","first_name":"Magdalena","last_name":"Andrae"},{"full_name":"Villányi, Márton","orcid":"0000-0001-8126-0426","id":"3FFCCD3A-F248-11E8-B48F-1D18A9856A87","first_name":"Márton","last_name":"Villányi"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"mla":"Andrae, Magdalena, and Márton Villányi. “Der Springer Compact-Deal – Ein Erster Einblick in Die Evaluierung Einer Offsetting-Vereinbarung.” Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare, vol. 70, no. 2, VÖB, 2017, pp. 274–80, doi:10.31263/voebm.v70i2.1898.","chicago":"Andrae, Magdalena, and Márton Villányi. “Der Springer Compact-Deal – Ein Erster Einblick in Die Evaluierung Einer Offsetting-Vereinbarung.” Mitteilungen Der Vereinigung Österreichischer Bibliothekarinnen Und Bibliothekare. 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Villányi, “Der Springer Compact-Deal – Ein erster Einblick in die Evaluierung einer Offsetting-Vereinbarung,” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen und Bibliothekare, vol. 70, no. 2. VÖB, pp. 274–280, 2017."},"volume":70,"type":"journal_article","status":"public","publication_status":"published","department":[{"_id":"E-Lib"}],"_id":"807","date_published":"2017-08-01T00:00:00Z","month":"08","year":"2017","date_updated":"2021-01-12T08:16:45Z","day":"01"},{"ddc":["020"],"oa":1,"date_created":"2018-12-11T11:48:42Z","issue":"2","oa_version":"Published Version","title":"Metadata for research data in practice","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"VÖB","page":"200 - 207","abstract":[{"lang":"eng","text":"What data is needed about data? Describing the process to answer this question for the institutional data repository IST DataRep."}],"file":[{"relation":"main_file","access_level":"open_access","file_size":7843975,"checksum":"7c4544d07efa2c2add8612b489abb4e2","creator":"dernst","date_updated":"2020-07-14T12:48:11Z","file_name":"2017_VOEB_Petritsch.pdf","date_created":"2019-01-18T13:32:17Z","file_id":"5850","content_type":"application/pdf"}],"has_accepted_license":"1","scopus_import":1,"language":[{"iso":"eng"}],"intvolume":" 70","publication_identifier":{"issn":["10222588"]},"publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare","doi":"10.31263/voebm.v70i2.1678","file_date_updated":"2020-07-14T12:48:11Z","author":[{"first_name":"Barbara","id":"406048EC-F248-11E8-B48F-1D18A9856A87","last_name":"Petritsch","full_name":"Petritsch, Barbara","orcid":"0000-0003-2724-4614"}],"publist_id":"6823","citation":{"apa":"Petritsch, B. (2017). Metadata for research data in practice. 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In: AHPC17 – Austrian HPC Meeting 2017. FSP Scientific Computing; 2017:28.","ista":"Schlögl A, Kiss J. 2017. Scientific Computing at IST Austria. AHPC17 – Austrian HPC Meeting 2017. AHPC: Austrian HPC Meeting, 28.","apa":"Schlögl, A., & Kiss, J. (2017). Scientific Computing at IST Austria. In AHPC17 – Austrian HPC Meeting 2017 (p. 28). Grundlsee, Austria: FSP Scientific Computing.","short":"A. Schlögl, J. Kiss, in:, AHPC17 – Austrian HPC Meeting 2017, FSP Scientific Computing, 2017, p. 28.","ieee":"A. Schlögl and J. Kiss, “Scientific Computing at IST Austria,” in AHPC17 – Austrian HPC Meeting 2017, Grundlsee, Austria, 2017, p. 28."},"main_file_link":[{"url":"https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc17/BOOKLET_AHPC17.pdf","open_access":"1"}],"conference":{"end_date":"2017-03-03","location":"Grundlsee, Austria","start_date":"2017-03-01","name":"AHPC: Austrian HPC Meeting"},"oa_version":"Published Version","publication_status":"published","publisher":"FSP Scientific Computing","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"conference_abstract","title":"Scientific Computing at IST Austria","oa":1,"ddc":["000"],"language":[{"iso":"eng"}],"publication":"AHPC17 – Austrian HPC Meeting 2017","author":[{"id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"full_name":"Kiss, Janos","last_name":"Kiss","id":"3D3A06F8-F248-11E8-B48F-1D18A9856A87","first_name":"Janos"}],"file_date_updated":"2023-05-16T07:20:50Z","date_created":"2023-05-05T12:58:53Z","date_updated":"2023-05-16T07:22:23Z","year":"2017","day":"03","_id":"12905","article_processing_charge":"No","department":[{"_id":"ScienComp"}],"has_accepted_license":"1","file":[{"access_level":"open_access","relation":"main_file","file_size":1005486,"creator":"dernst","checksum":"7bcc499479d4f4c5ce6c0071c24ca6c6","date_updated":"2023-05-16T07:20:50Z","file_name":"2017_AHPC_Schloegl.pdf","date_created":"2023-05-16T07:20:50Z","file_id":"12969","content_type":"application/pdf","success":1}],"page":"28","month":"03","date_published":"2017-03-03T00:00:00Z"},{"issue":"6","publisher":"American Chemical Society","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Current-phase relation of ballistic graphene Josephson junctions","oa_version":"Published Version","quality_controlled":"1","oa":1,"ddc":["621"],"date_created":"2018-12-11T11:49:33Z","external_id":{"isi":["000403631600011"]},"scopus_import":"1","page":"3396 - 3401","file":[{"file_size":508638,"relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:48:18Z","checksum":"22021daa90cf13b01becd776838acb7b","creator":"system","content_type":"application/pdf","file_id":"5037","file_name":"IST-2017-826-v1+1_2017_Aguilera-Servin_Current.pdf","date_created":"2018-12-12T10:13:50Z"}],"has_accepted_license":"1","abstract":[{"text":"The current-phase relation (CPR) of a Josephson junction (JJ) determines how the supercurrent evolves with the superconducting phase difference across the junction. Knowledge of the CPR is essential in order to understand the response of a JJ to various external parameters. Despite the rising interest in ultraclean encapsulated graphene JJs, the CPR of such junctions remains unknown. Here, we use a fully gate-tunable graphene superconducting quantum intereference device (SQUID) to determine the CPR of ballistic graphene JJs. Each of the two JJs in the SQUID is made with graphene encapsulated in hexagonal boron nitride. By independently controlling the critical current of the JJs, we can operate the SQUID either in a symmetric or asymmetric configuration. The highly asymmetric SQUID allows us to phase-bias one of the JJs and thereby directly obtain its CPR. The CPR is found to be skewed, deviating significantly from a sinusoidal form. The skewness can be tuned with the gate voltage and oscillates in antiphase with Fabry-Pérot resistance oscillations of the ballistic graphene cavity. We compare our experiments with tight-binding calculations that include realistic graphene-superconductor interfaces and find a good qualitative agreement.","lang":"eng"}],"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"citation":{"ista":"Nanda G, Aguilera Servin JL, Rakyta P, Kormányos A, Kleiner R, Koelle D, Watanabe K, Taniguchi T, Vandersypen L, Goswami S. 2017. Current-phase relation of ballistic graphene Josephson junctions. Nano Letters. 17(6), 3396–3401.","ama":"Nanda G, Aguilera Servin JL, Rakyta P, et al. Current-phase relation of ballistic graphene Josephson junctions. Nano Letters. 2017;17(6):3396-3401. doi:10.1021/acs.nanolett.7b00097","mla":"Nanda, Gaurav, et al. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.” Nano Letters, vol. 17, no. 6, American Chemical Society, 2017, pp. 3396–401, doi:10.1021/acs.nanolett.7b00097.","chicago":"Nanda, Gaurav, Juan L Aguilera Servin, Péter Rakyta, Andor Kormányos, Reinhold Kleiner, Dieter Koelle, Kazuo Watanabe, Takashi Taniguchi, Lieven Vandersypen, and Srijit Goswami. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.” Nano Letters. American Chemical Society, 2017. https://doi.org/10.1021/acs.nanolett.7b00097.","ieee":"G. Nanda et al., “Current-phase relation of ballistic graphene Josephson junctions,” Nano Letters, vol. 17, no. 6. American Chemical Society, pp. 3396–3401, 2017.","apa":"Nanda, G., Aguilera Servin, J. L., Rakyta, P., Kormányos, A., Kleiner, R., Koelle, D., … Goswami, S. (2017). Current-phase relation of ballistic graphene Josephson junctions. Nano Letters. American Chemical Society. https://doi.org/10.1021/acs.nanolett.7b00097","short":"G. Nanda, J.L. Aguilera Servin, P. Rakyta, A. Kormányos, R. Kleiner, D. Koelle, K. Watanabe, T. Taniguchi, L. Vandersypen, S. Goswami, Nano Letters 17 (2017) 3396–3401."},"status":"public","volume":17,"type":"journal_article","publication_status":"published","pubrep_id":"826","doi":"10.1021/acs.nanolett.7b00097","publication":"Nano Letters","publication_identifier":{"issn":["15306984"]},"intvolume":" 17","language":[{"iso":"eng"}],"publist_id":"6412","author":[{"full_name":"Nanda, Gaurav","first_name":"Gaurav","last_name":"Nanda"},{"orcid":"0000-0002-2862-8372","full_name":"Aguilera Servin, Juan L","last_name":"Aguilera Servin","id":"2A67C376-F248-11E8-B48F-1D18A9856A87","first_name":"Juan L"},{"full_name":"Rakyta, Péter","first_name":"Péter","last_name":"Rakyta"},{"full_name":"Kormányos, Andor","last_name":"Kormányos","first_name":"Andor"},{"full_name":"Kleiner, Reinhold","first_name":"Reinhold","last_name":"Kleiner"},{"last_name":"Koelle","first_name":"Dieter","full_name":"Koelle, Dieter"},{"last_name":"Watanabe","first_name":"Kazuo","full_name":"Watanabe, Kazuo"},{"first_name":"Takashi","last_name":"Taniguchi","full_name":"Taniguchi, Takashi"},{"last_name":"Vandersypen","first_name":"Lieven","full_name":"Vandersypen, Lieven"},{"last_name":"Goswami","first_name":"Srijit","full_name":"Goswami, Srijit"}],"file_date_updated":"2020-07-14T12:48:18Z","year":"2017","date_updated":"2023-09-22T09:56:21Z","day":"05","_id":"988","isi":1,"department":[{"_id":"NanoFab"}],"month":"05","date_published":"2017-05-05T00:00:00Z"},{"external_id":{"isi":["000411331800020"]},"scopus_import":"1","project":[{"name":"Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments","grant_number":"LS13-029","_id":"25AD6156-B435-11E9-9278-68D0E5697425"},{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7"}],"article_processing_charge":"No","page":"188 - 200","abstract":[{"text":"Actin filaments polymerizing against membranes power endocytosis, vesicular traffic, and cell motility. In vitro reconstitution studies suggest that the structure and the dynamics of actin networks respond to mechanical forces. We demonstrate that lamellipodial actin of migrating cells responds to mechanical load when membrane tension is modulated. In a steady state, migrating cell filaments assume the canonical dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension triggers a dense network with a broadened range of angles, whereas decreased tension causes a shift to a sparse configuration dominated by filaments growing perpendicularly to the plasma membrane. We show that these responses emerge from the geometry of branched actin: when load per filament decreases, elongation speed increases and perpendicular filaments gradually outcompete others because they polymerize the shortest distance to the membrane, where they are protected from capping. This network-intrinsic geometrical adaptation mechanism tunes protrusive force in response to mechanical load.","lang":"eng"}],"issue":"1","quality_controlled":"1","oa_version":"None","acknowledged_ssus":[{"_id":"ScienComp"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Cell Press","title":"Load adaptation of lamellipodial actin networks","ec_funded":1,"date_created":"2018-12-11T11:48:10Z","date_updated":"2023-09-28T11:33:49Z","year":"2017","day":"21","_id":"727","department":[{"_id":"MiSi"},{"_id":"Bio"}],"isi":1,"month":"09","date_published":"2017-09-21T00:00:00Z","citation":{"apa":"Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A., Winkler, C., … Sixt, M. K. (2017). Load adaptation of lamellipodial actin networks. Cell. Cell Press. https://doi.org/10.1016/j.cell.2017.07.051","short":"J. Mueller, G. Szep, M. Nemethova, I. de Vries, A. Lieber, C. Winkler, K. Kruse, J. Small, C. Schmeiser, K. Keren, R. Hauschild, M.K. Sixt, Cell 171 (2017) 188–200.","ieee":"J. Mueller et al., “Load adaptation of lamellipodial actin networks,” Cell, vol. 171, no. 1. Cell Press, pp. 188–200, 2017.","mla":"Mueller, Jan, et al. “Load Adaptation of Lamellipodial Actin Networks.” Cell, vol. 171, no. 1, Cell Press, 2017, pp. 188–200, doi:10.1016/j.cell.2017.07.051.","chicago":"Mueller, Jan, Gregory Szep, Maria Nemethova, Ingrid de Vries, Arnon Lieber, Christoph Winkler, Karsten Kruse, et al. “Load Adaptation of Lamellipodial Actin Networks.” Cell. Cell Press, 2017. https://doi.org/10.1016/j.cell.2017.07.051.","ista":"Mueller J, Szep G, Nemethova M, de Vries I, Lieber A, Winkler C, Kruse K, Small J, Schmeiser C, Keren K, Hauschild R, Sixt MK. 2017. Load adaptation of lamellipodial actin networks. Cell. 171(1), 188–200.","ama":"Mueller J, Szep G, Nemethova M, et al. Load adaptation of lamellipodial actin networks. Cell. 2017;171(1):188-200. doi:10.1016/j.cell.2017.07.051"},"publication_status":"published","status":"public","volume":171,"type":"journal_article","publication_identifier":{"issn":["00928674"]},"language":[{"iso":"eng"}],"intvolume":" 171","doi":"10.1016/j.cell.2017.07.051","publication":"Cell","author":[{"first_name":"Jan","last_name":"Mueller","full_name":"Mueller, Jan"},{"full_name":"Szep, Gregory","first_name":"Gregory","id":"4BFB7762-F248-11E8-B48F-1D18A9856A87","last_name":"Szep"},{"first_name":"Maria","id":"34E27F1C-F248-11E8-B48F-1D18A9856A87","last_name":"Nemethova","full_name":"Nemethova, Maria"},{"last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","full_name":"De Vries, Ingrid"},{"full_name":"Lieber, Arnon","last_name":"Lieber","first_name":"Arnon"},{"full_name":"Winkler, Christoph","last_name":"Winkler","first_name":"Christoph"},{"full_name":"Kruse, Karsten","first_name":"Karsten","last_name":"Kruse"},{"full_name":"Small, John","first_name":"John","last_name":"Small"},{"full_name":"Schmeiser, Christian","first_name":"Christian","last_name":"Schmeiser"},{"first_name":"Kinneret","last_name":"Keren","full_name":"Keren, Kinneret"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"publist_id":"6951"},{"title":"Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Optica Publishing Group","quality_controlled":"1","oa_version":"None","issue":"10","date_created":"2018-12-11T11:47:51Z","article_type":"original","ddc":["530"],"scopus_import":"1","abstract":[{"lang":"eng","text":"We report the enhancement of infrared absorption of chemisorbed carbon monoxide on platinum in the gap of plasmonic nanoantennas. Our method is based on the self-assembled formation of platinum nanoislands on nanoscopic dipole antenna arrays manufactured via electron beam lithography. We employ systematic variations of the plasmonic antenna resonance to precisely couple to the molecular stretch vibration of carbon monoxide adsorbed on the platinum nanoislands. Ultimately, we reach more than 1500-fold infrared absorption enhancements, allowing for an ultrasensitive detection of a monolayer of chemisorbed carbon monoxide. The developed procedure can be adapted to other metal adsorbents and molecular species and could be utilized for coverage sensing in surface catalytic reactions. "}],"page":"1931 - 1934","article_processing_charge":"No","volume":42,"type":"journal_article","status":"public","publication_status":"published","citation":{"ista":"Haase J, Bagiante S, Sigg H, Van Bokhoven J. 2017. Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. Optics Letters. 42(10), 1931–1934.","ama":"Haase J, Bagiante S, Sigg H, Van Bokhoven J. Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. Optics Letters. 2017;42(10):1931-1934. doi:10.1364/OL.42.001931","mla":"Haase, Johannes, et al. “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic Nanoantennas.” Optics Letters, vol. 42, no. 10, Optica Publishing Group, 2017, pp. 1931–34, doi:10.1364/OL.42.001931.","chicago":"Haase, Johannes, Salvatore Bagiante, Hans Sigg, and Jeroen Van Bokhoven. “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic Nanoantennas.” Optics Letters. Optica Publishing Group, 2017. https://doi.org/10.1364/OL.42.001931.","ieee":"J. Haase, S. Bagiante, H. Sigg, and J. Van Bokhoven, “Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas,” Optics Letters, vol. 42, no. 10. Optica Publishing Group, pp. 1931–1934, 2017.","short":"J. Haase, S. Bagiante, H. Sigg, J. Van Bokhoven, Optics Letters 42 (2017) 1931–1934.","apa":"Haase, J., Bagiante, S., Sigg, H., & Van Bokhoven, J. (2017). Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. Optics Letters. Optica Publishing Group. https://doi.org/10.1364/OL.42.001931"},"publist_id":"7048","author":[{"first_name":"Johannes","last_name":"Haase","full_name":"Haase, Johannes"},{"orcid":"0000-0002-0122-9603","full_name":"Bagiante, Salvatore","last_name":"Bagiante","first_name":"Salvatore","id":"38ED402E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Hans","last_name":"Sigg","full_name":"Sigg, Hans"},{"full_name":"Van Bokhoven, Jeroen","last_name":"Van Bokhoven","first_name":"Jeroen"}],"publication":"Optics Letters","doi":"10.1364/OL.42.001931","language":[{"iso":"eng"}],"intvolume":" 42","day":"15","year":"2017","date_updated":"2023-10-17T12:16:02Z","date_published":"2017-05-15T00:00:00Z","month":"05","department":[{"_id":"NanoFab"}],"_id":"675"},{"publist_id":"6360","file_date_updated":"2018-12-12T10:08:20Z","author":[{"last_name":"Villányi","id":"3FFCCD3A-F248-11E8-B48F-1D18A9856A87","first_name":"Márton","orcid":"0000-0001-8126-0426","full_name":"Villányi, Márton"}],"publication":"Informationspraxis","doi":"10.11588/ip.2017.1.35227","language":[{"iso":"eng"}],"intvolume":" 3","publication_identifier":{"issn":["2297-3249"]},"volume":3,"type":"journal_article","status":"public","pubrep_id":"799","publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"short":"M. Villányi, Informationspraxis 3 (2017).","apa":"Villányi, M. (2017). Ein freies Bibliothekssystem für wissenschaftliche Bibliotheken – Werkstattbericht der IST Austria Library. Informationspraxis. Verein Informationspraxis . https://doi.org/10.11588/ip.2017.1.35227","ieee":"M. Villányi, “Ein freies Bibliothekssystem für wissenschaftliche Bibliotheken – Werkstattbericht der IST Austria Library,” Informationspraxis, vol. 3, no. 1. Verein Informationspraxis , 2017.","chicago":"Villányi, Márton. “Ein Freies Bibliothekssystem Für Wissenschaftliche Bibliotheken – Werkstattbericht Der IST Austria Library.” Informationspraxis. Verein Informationspraxis , 2017. https://doi.org/10.11588/ip.2017.1.35227.","mla":"Villányi, Márton. “Ein Freies Bibliothekssystem Für Wissenschaftliche Bibliotheken – Werkstattbericht Der IST Austria Library.” Informationspraxis, vol. 3, no. 1, Verein Informationspraxis , 2017, doi:10.11588/ip.2017.1.35227.","ama":"Villányi M. Ein freies Bibliothekssystem für wissenschaftliche Bibliotheken – Werkstattbericht der IST Austria Library. Informationspraxis. 2017;3(1). doi:10.11588/ip.2017.1.35227","ista":"Villányi M. 2017. Ein freies Bibliothekssystem für wissenschaftliche Bibliotheken – Werkstattbericht der IST Austria Library. Informationspraxis. 3(1)."},"date_published":"2017-01-01T00:00:00Z","month":"01","department":[{"_id":"E-Lib"}],"_id":"1030","day":"01","year":"2017","date_updated":"2023-10-18T07:49:29Z","date_created":"2018-12-11T11:49:46Z","article_type":"original","ddc":["020"],"oa":1,"title":"Ein freies Bibliothekssystem für wissenschaftliche Bibliotheken – Werkstattbericht der IST Austria Library","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Verein Informationspraxis ","oa_version":"Published Version","issue":"1","popular_science":"1","has_accepted_license":"1","abstract":[{"lang":"ger","text":"Auf der Suche nach einem Bibliothekssystem entschied sich die Forschungseinrichtung IST Austria im Jahr 2014 für das Open-Source-Produkt Koha. In einem ersten Schritt wurden zunächst Grundfunktionen aktiviert um im Anschluss diverse zusätzliche Tools zum Einsatz zu bringen. Die große Flexibilität des Systems erlaubt maßgeschneiderte Lösungen für unterschiedlichste Institutionen. Trotz Herausforderungen kann die Bibliothek auf eine erfolgreiche Implementierung zurückblicken."},{"lang":"eng","text":"IST Austria was looking for a new library system until 2014 when the research institute decided\r\nto implement Koha. The library first activated basic functions of the open-source product and\r\nthen brought additional tools into operation. The high flexibility of the system allows customized\r\nsolutions for different institutions. Although the library faced some challenges, it can now look\r\nback on a successful implementation."}],"file":[{"date_updated":"2018-12-12T10:08:20Z","creator":"system","file_size":201163,"access_level":"open_access","relation":"main_file","file_id":"4680","content_type":"application/pdf","date_created":"2018-12-12T10:08:20Z","file_name":"IST-2017-799-v1+1_35227-112025-1-PB.pdf"}],"article_processing_charge":"No"},{"date_created":"2018-12-12T12:31:35Z","keyword":["Cell migration","tracking","forward migration index","FMI"],"file_date_updated":"2020-07-14T12:47:04Z","author":[{"last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"}],"doi":"10.15479/AT:ISTA:75","ddc":["570"],"oa":1,"type":"research_data","title":"Forward migration indexes","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Institute of Science and Technology Austria","oa_version":"Published Version","tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)","short":"CC0 (1.0)"},"citation":{"ieee":"R. Hauschild, “Forward migration indexes.” Institute of Science and Technology Austria, 2017.","short":"R. Hauschild, (2017).","apa":"Hauschild, R. (2017). Forward migration indexes. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:75","ama":"Hauschild R. Forward migration indexes. 2017. doi:10.15479/AT:ISTA:75","ista":"Hauschild R. 2017. Forward migration indexes, Institute of Science and Technology Austria, 10.15479/AT:ISTA:75.","chicago":"Hauschild, Robert. “Forward Migration Indexes.” Institute of Science and Technology Austria, 2017. https://doi.org/10.15479/AT:ISTA:75.","mla":"Hauschild, Robert. Forward Migration Indexes. Institute of Science and Technology Austria, 2017, doi:10.15479/AT:ISTA:75."},"date_published":"2017-10-04T00:00:00Z","month":"10","datarep_id":"75","file":[{"file_name":"IST-2017-75-v1+1_FMI.m","date_created":"2018-12-12T13:02:29Z","file_id":"5596","content_type":"application/octet-stream","relation":"main_file","access_level":"open_access","file_size":799,"checksum":"cb7a2fa622460eca6231d659ce590e32","creator":"system","date_updated":"2020-07-14T12:47:04Z"}],"abstract":[{"text":"Matlab script to calculate the forward migration indexes (/) from TrackMate spot-statistics files.","lang":"eng"}],"has_accepted_license":"1","department":[{"_id":"Bio"}],"article_processing_charge":"No","_id":"5570","day":"04","year":"2017","date_updated":"2024-02-21T13:47:14Z"},{"date_updated":"2024-02-21T13:49:00Z","year":"2017","related_material":{"record":[{"status":"public","id":"665","relation":"research_paper"}]},"day":"10","_id":"5560","article_processing_charge":"No","department":[{"_id":"CaGu"},{"_id":"GaTk"},{"_id":"Bio"}],"has_accepted_license":"1","file":[{"access_level":"open_access","relation":"main_file","file_size":6773204,"checksum":"d77859af757ac8025c50c7b12b52eaf3","creator":"system","date_updated":"2020-07-14T12:47:03Z","content_type":"application/zip","file_id":"5603","file_name":"IST-2017-53-v1+1_Data_MDE.zip","date_created":"2018-12-12T13:02:38Z"}],"abstract":[{"text":"This repository contains the data collected for the manuscript \"Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity\".\r\nThe data is compressed into a single archive. Within the archive, different folders correspond to figures of the main text and the SI of the related publication.\r\nData is saved as plain text, with each folder containing a separate readme file describing the format. Typically, the data is from fluorescence microscopy measurements of single cells growing in a microfluidic \"mother machine\" device, and consists of relevant values (primarily arbitrary unit or normalized fluorescence measurements, and division times / growth rates) after raw microscopy images have been processed, segmented, and their features extracted, as described in the methods section of the related publication.","lang":"eng"}],"datarep_id":"53","month":"03","date_published":"2017-03-10T00:00:00Z","citation":{"chicago":"Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza, Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning of the Multi-Drug Efflux Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity.” Institute of Science and Technology Austria, 2017. https://doi.org/10.15479/AT:ISTA:53.","mla":"Bergmiller, Tobias, et al. Biased Partitioning of the Multi-Drug Efflux Pump AcrAB-TolC Underlies Long-Lived Phenotypic Heterogeneity. Institute of Science and Technology Austria, 2017, doi:10.15479/AT:ISTA:53.","ama":"Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity. 2017. doi:10.15479/AT:ISTA:53","ista":"Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik G, Guet CC. 2017. Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity, Institute of Science and Technology Austria, 10.15479/AT:ISTA:53.","short":"T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild, G. Tkačik, C.C. Guet, (2017).","apa":"Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild, R., … Guet, C. C. (2017). Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:53","ieee":"T. Bergmiller et al., “Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity.” Institute of Science and Technology Austria, 2017."},"tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)","short":"CC0 (1.0)"},"oa_version":"Published Version","publisher":"Institute of Science and Technology Austria","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","type":"research_data","title":"Biased partitioning of the multi-drug efflux pump AcrAB-TolC underlies long-lived phenotypic heterogeneity","oa":1,"ddc":["571"],"doi":"10.15479/AT:ISTA:53","author":[{"last_name":"Bergmiller","id":"2C471CFA-F248-11E8-B48F-1D18A9856A87","first_name":"Tobias","orcid":"0000-0001-5396-4346","full_name":"Bergmiller, Tobias"},{"full_name":"Andersson, Anna M","orcid":"0000-0003-2912-6769","id":"2B8A40DA-F248-11E8-B48F-1D18A9856A87","first_name":"Anna M","last_name":"Andersson"},{"first_name":"Kathrin","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","last_name":"Tomasek","full_name":"Tomasek, Kathrin","orcid":"0000-0003-3768-877X"},{"last_name":"Balleza","first_name":"Enrique","full_name":"Balleza, Enrique"},{"full_name":"Kiviet, Daniel","last_name":"Kiviet","first_name":"Daniel"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","first_name":"Gasper","last_name":"Tkacik","full_name":"Tkacik, Gasper","orcid":"0000-0002-6699-1455"},{"id":"47F8433E-F248-11E8-B48F-1D18A9856A87","first_name":"Calin C","last_name":"Guet","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052"}],"file_date_updated":"2020-07-14T12:47:03Z","keyword":["single cell microscopy","mother machine microfluidic device","AcrAB-TolC pump","multi-drug efflux","Escherichia coli"],"date_created":"2018-12-12T12:31:32Z"},{"date_published":"2017-04-21T00:00:00Z","month":"04","department":[{"_id":"CaGu"},{"_id":"GaTk"},{"_id":"Bio"}],"_id":"665","related_material":{"record":[{"id":"5560","relation":"popular_science","status":"public"}]},"day":"21","date_updated":"2024-02-21T13:49:00Z","year":"2017","author":[{"first_name":"Tobias","id":"2C471CFA-F248-11E8-B48F-1D18A9856A87","last_name":"Bergmiller","full_name":"Bergmiller, Tobias","orcid":"0000-0001-5396-4346"},{"first_name":"Anna M","id":"2B8A40DA-F248-11E8-B48F-1D18A9856A87","last_name":"Andersson","full_name":"Andersson, Anna M","orcid":"0000-0003-2912-6769"},{"orcid":"0000-0003-3768-877X","full_name":"Tomasek, Kathrin","last_name":"Tomasek","first_name":"Kathrin","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Balleza","first_name":"Enrique","full_name":"Balleza, Enrique"},{"first_name":"Daniel","last_name":"Kiviet","full_name":"Kiviet, Daniel"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"},{"full_name":"Tkacik, Gasper","orcid":"0000-0002-6699-1455","first_name":"Gasper","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","last_name":"Tkacik"},{"id":"47F8433E-F248-11E8-B48F-1D18A9856A87","first_name":"Calin C","last_name":"Guet","full_name":"Guet, Calin C","orcid":"0000-0001-6220-2052"}],"publist_id":"7064","intvolume":" 356","language":[{"iso":"eng"}],"publication_identifier":{"issn":["00368075"]},"publication":"Science","doi":"10.1126/science.aaf4762","publication_status":"published","type":"journal_article","volume":356,"status":"public","citation":{"short":"T. Bergmiller, A.M. Andersson, K. Tomasek, E. Balleza, D. Kiviet, R. Hauschild, G. Tkačik, C.C. Guet, Science 356 (2017) 311–315.","apa":"Bergmiller, T., Andersson, A. M., Tomasek, K., Balleza, E., Kiviet, D., Hauschild, R., … Guet, C. C. (2017). Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.aaf4762","ieee":"T. Bergmiller et al., “Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity,” Science, vol. 356, no. 6335. American Association for the Advancement of Science, pp. 311–315, 2017.","chicago":"Bergmiller, Tobias, Anna M Andersson, Kathrin Tomasek, Enrique Balleza, Daniel Kiviet, Robert Hauschild, Gašper Tkačik, and Calin C Guet. “Biased Partitioning of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” Science. American Association for the Advancement of Science, 2017. https://doi.org/10.1126/science.aaf4762.","mla":"Bergmiller, Tobias, et al. “Biased Partitioning of the Multidrug Efflux Pump AcrAB TolC Underlies Long Lived Phenotypic Heterogeneity.” Science, vol. 356, no. 6335, American Association for the Advancement of Science, 2017, pp. 311–15, doi:10.1126/science.aaf4762.","ista":"Bergmiller T, Andersson AM, Tomasek K, Balleza E, Kiviet D, Hauschild R, Tkačik G, Guet CC. 2017. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. 356(6335), 311–315.","ama":"Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. Science. 2017;356(6335):311-315. doi:10.1126/science.aaf4762"},"article_processing_charge":"No","project":[{"grant_number":"P28844-B27","_id":"254E9036-B435-11E9-9278-68D0E5697425","name":"Biophysics of information processing in gene regulation","call_identifier":"FWF"}],"abstract":[{"text":"The molecular mechanisms underlying phenotypic variation in isogenic bacterial populations remain poorly understood.We report that AcrAB-TolC, the main multidrug efflux pump of Escherichia coli, exhibits a strong partitioning bias for old cell poles by a segregation mechanism that is mediated by ternary AcrAB-TolC complex formation. Mother cells inheriting old poles are phenotypically distinct and display increased drug efflux activity relative to daughters. Consequently, we find systematic and long-lived growth differences between mother and daughter cells in the presence of subinhibitory drug concentrations. A simple model for biased partitioning predicts a population structure of long-lived and highly heterogeneous phenotypes. This straightforward mechanism of generating sustained growth rate differences at subinhibitory antibiotic concentrations has implications for understanding the emergence of multidrug resistance in bacteria.","lang":"eng"}],"page":"311 - 315","scopus_import":1,"date_created":"2018-12-11T11:47:48Z","article_type":"original","quality_controlled":"1","oa_version":"None","title":"Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"American Association for the Advancement of Science","issue":"6335"},{"acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","doi":"10.7554/eLife.26792","publication":"eLife","intvolume":" 6","language":[{"iso":"eng"}],"publist_id":"6471","author":[{"orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","first_name":"Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-9767-8699","full_name":"Fendrych, Matyas","last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","first_name":"Matyas"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","last_name":"Barone","full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"file_date_updated":"2020-07-14T12:48:15Z","citation":{"ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792.","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 2017;6. doi:10.7554/eLife.26792","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” ELife, vol. 6, e26792, eLife Sciences Publications, 2017, doi:10.7554/eLife.26792.","chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” ELife. eLife Sciences Publications, 2017. https://doi.org/10.7554/eLife.26792.","ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” eLife, vol. 6. eLife Sciences Publications, 2017.","short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., & Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.26792"},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","type":"journal_article","volume":6,"pubrep_id":"847","publication_status":"published","_id":"946","isi":1,"department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"month":"06","date_published":"2017-06-19T00:00:00Z","article_number":"e26792","year":"2017","date_updated":"2024-02-21T13:49:34Z","day":"19","related_material":{"record":[{"relation":"popular_science","id":"5566","status":"public"}]},"oa":1,"ddc":["570"],"ec_funded":1,"date_created":"2018-12-11T11:49:21Z","publisher":"eLife Sciences Publications","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","quality_controlled":"1","oa_version":"Published Version","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes."}],"file":[{"file_id":"5315","content_type":"application/pdf","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","date_created":"2018-12-12T10:17:57Z","date_updated":"2020-07-14T12:48:15Z","checksum":"9af3398cb0d81f99d79016a616df22e9","creator":"system","file_size":19581847,"relation":"main_file","access_level":"open_access"}],"project":[{"call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"name":"Molecular basis of root growth inhibition by auxin","call_identifier":"FWF","_id":"2572ED28-B435-11E9-9278-68D0E5697425","grant_number":"M02128"},{"grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF"},{"grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"}],"article_processing_charge":"Yes","external_id":{"isi":["000404728300001"]},"scopus_import":"1"},{"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"oa_version":"Published Version","title":"Light sheet fluorescence microscopy of plant roots growing on the surface of a gel","publisher":"Journal of Visualized Experiments","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","issue":"119","date_created":"2018-12-11T11:50:01Z","ec_funded":1,"ddc":["580"],"oa":1,"scopus_import":"1","external_id":{"isi":["000397847200041"]},"article_processing_charge":"No","project":[{"name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734"},{"name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. "}],"file":[{"date_updated":"2018-12-12T10:16:31Z","creator":"system","file_size":57678,"relation":"main_file","access_level":"open_access","file_name":"IST-2017-808-v1+1_2017_VWangenheim_list.pdf","date_created":"2018-12-12T10:16:31Z","content_type":"application/pdf","file_id":"5219"},{"file_id":"5220","content_type":"application/pdf","file_name":"IST-2017-808-v1+2_2017_VWangenheim_article.pdf","date_created":"2018-12-12T10:16:32Z","access_level":"open_access","relation":"main_file","file_size":1317820,"creator":"system","date_updated":"2018-12-12T10:16:32Z"}],"publication_status":"published","pubrep_id":"808","volume":2017,"type":"journal_article","status":"public","citation":{"mla":"von Wangenheim, Daniel, et al. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Journal of Visualized Experiments JoVE, vol. 2017, no. 119, e55044, Journal of Visualized Experiments, 2017, doi:10.3791/55044.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Journal of Visualized Experiments JoVE. Journal of Visualized Experiments, 2017. https://doi.org/10.3791/55044.","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of visualized experiments JoVE. 2017(119), e55044.","ama":"von Wangenheim D, Hauschild R, Friml J. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of visualized experiments JoVE. 2017;2017(119). doi:10.3791/55044","short":"D. von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments JoVE 2017 (2017).","apa":"von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of Visualized Experiments JoVE. Journal of Visualized Experiments. https://doi.org/10.3791/55044","ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light sheet fluorescence microscopy of plant roots growing on the surface of a gel,” Journal of visualized experiments JoVE, vol. 2017, no. 119. Journal of Visualized Experiments, 2017."},"file_date_updated":"2018-12-12T10:16:32Z","author":[{"full_name":"Von Wangenheim, Daniel","orcid":"0000-0002-6862-1247","first_name":"Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87","last_name":"Von Wangenheim"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"publist_id":"6302","language":[{"iso":"eng"}],"intvolume":" 2017","publication":"Journal of visualized experiments JoVE","doi":"10.3791/55044","related_material":{"record":[{"status":"public","id":"5565","relation":"popular_science"}]},"day":"18","date_updated":"2024-02-21T13:49:12Z","year":"2017","article_number":"e55044","date_published":"2017-01-18T00:00:00Z","month":"01","isi":1,"department":[{"_id":"JiFr"},{"_id":"Bio"}],"_id":"1078"},{"status":"public","publisher":"Institute of Science and Technology Austria","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel","type":"research_data","oa_version":"Published Version","citation":{"ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel.” Institute of Science and Technology Austria, 2017.","short":"D. von Wangenheim, R. Hauschild, J. Friml, (2017).","apa":"von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:66","ama":"von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. 2017. doi:10.15479/AT:ISTA:66","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, Institute of Science and Technology Austria, 10.15479/AT:ISTA:66.","mla":"von Wangenheim, Daniel, et al. Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel. Institute of Science and Technology Austria, 2017, doi:10.15479/AT:ISTA:66.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute of Science and Technology Austria, 2017. https://doi.org/10.15479/AT:ISTA:66."},"ec_funded":1,"publist_id":"6302","date_created":"2018-12-12T12:31:34Z","author":[{"orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","first_name":"Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"file_date_updated":"2020-07-14T12:47:03Z","acknowledgement":"fund: FP7-ERC 0101109","doi":"10.15479/AT:ISTA:66","oa":1,"ddc":["580"],"day":"10","related_material":{"record":[{"status":"public","relation":"research_paper","id":"1078"}]},"year":"2017","date_updated":"2024-02-21T13:49:13Z","month":"04","date_published":"2017-04-10T00:00:00Z","datarep_id":"66","abstract":[{"lang":"eng","text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. \r\nThe Video is licensed under a CC BY NC ND license. "}],"file":[{"date_updated":"2020-07-14T12:47:03Z","creator":"system","checksum":"b7552fc23540a85dc5a22fd4484eae71","file_size":101497758,"relation":"main_file","access_level":"open_access","file_name":"IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4","date_created":"2018-12-12T13:02:33Z","content_type":"video/mp4","file_id":"5599"}],"has_accepted_license":"1","_id":"5565","project":[{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"}],"department":[{"_id":"JiFr"},{"_id":"Bio"}],"article_processing_charge":"No"},{"citation":{"apa":"Hauschild, R. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:69","short":"R. Hauschild, (2017).","ieee":"R. Hauschild, “Live tracking of moving samples in confocal microscopy for vertically grown roots.” Institute of Science and Technology Austria, 2017.","chicago":"Hauschild, Robert. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” Institute of Science and Technology Austria, 2017. https://doi.org/10.15479/AT:ISTA:69.","mla":"Hauschild, Robert. Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots. Institute of Science and Technology Austria, 2017, doi:10.15479/AT:ISTA:69.","ista":"Hauschild R. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots, Institute of Science and Technology Austria, 10.15479/AT:ISTA:69.","ama":"Hauschild R. Live tracking of moving samples in confocal microscopy for vertically grown roots. 2017. doi:10.15479/AT:ISTA:69"},"tmp":{"short":"CC BY-SA (4.0)","image":"/images/cc_by_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)"},"oa_version":"Published Version","publisher":"Institute of Science and Technology Austria","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"research_data","title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","oa":1,"ddc":["570"],"doi":"10.15479/AT:ISTA:69","author":[{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild"}],"file_date_updated":"2020-07-14T12:47:04Z","keyword":["tool","tracking","confocal microscopy"],"date_created":"2018-12-12T12:31:34Z","date_updated":"2024-02-21T13:49:34Z","year":"2017","license":"https://creativecommons.org/licenses/by-sa/4.0/","related_material":{"record":[{"id":"946","relation":"research_paper","status":"public"}]},"day":"21","_id":"5566","article_processing_charge":"No","department":[{"_id":"Bio"}],"abstract":[{"text":"Current minimal version of TipTracker","lang":"eng"}],"file":[{"file_size":1587986,"relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:47:04Z","checksum":"a976000e6715106724a271cc9422be4a","creator":"system","date_created":"2018-12-12T13:04:12Z","file_name":"IST-2017-69-v1+2_TipTrackerZeissLSM700.zip","file_id":"5636","content_type":"application/zip"}],"has_accepted_license":"1","datarep_id":"69","month":"07","date_published":"2017-07-21T00:00:00Z"},{"issue":"10","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Company of Biologists","title":"Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation","oa_version":"Published Version","quality_controlled":"1","oa":1,"article_type":"original","ddc":["570"],"date_created":"2018-12-11T11:47:52Z","external_id":{"pmid":["28512197"]},"pmid":1,"scopus_import":1,"page":"1798 - 1806","has_accepted_license":"1","abstract":[{"lang":"eng","text":"The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation."}],"file":[{"creator":"dernst","checksum":"bc25125fb664706cdf180e061429f91d","date_updated":"2020-07-14T12:47:39Z","access_level":"open_access","relation":"main_file","file_size":8194516,"file_id":"6905","content_type":"application/pdf","file_name":"2017_Development_Krens.pdf","date_created":"2019-09-24T06:56:22Z"}],"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ama":"Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 2017;144(10):1798-1806. doi:10.1242/dev.144964","ista":"Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 144(10), 1798–1806.","chicago":"Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître, Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” Development. Company of Biologists, 2017. https://doi.org/10.1242/dev.144964.","mla":"Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” Development, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806, doi:10.1242/dev.144964.","ieee":"G. Krens et al., “Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation,” Development, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.","apa":"Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W., & Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. Company of Biologists. https://doi.org/10.1242/dev.144964","short":"G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J. Heisenberg, Development 144 (2017) 1798–1806."},"status":"public","volume":144,"type":"journal_article","publication_status":"published","doi":"10.1242/dev.144964","publication":"Development","publication_identifier":{"issn":["09501991"]},"intvolume":" 144","language":[{"iso":"eng"}],"publist_id":"7047","author":[{"full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","last_name":"Krens"},{"last_name":"Veldhuis","first_name":"Jim","full_name":"Veldhuis, Jim"},{"first_name":"Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367"},{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"Capek","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940"},{"first_name":"Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","last_name":"Maître","full_name":"Maître, Jean-Léon","orcid":"0000-0002-3688-1474"},{"full_name":"Brodland, Wayne","first_name":"Wayne","last_name":"Brodland"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"file_date_updated":"2020-07-14T12:47:39Z","year":"2017","date_updated":"2024-03-18T23:30:27Z","day":"15","related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"},{"status":"public","id":"50","relation":"dissertation_contains"}]},"_id":"676","department":[{"_id":"Bio"},{"_id":"CaHe"}],"month":"05","date_published":"2017-05-15T00:00:00Z"},{"publication":"Nature Cell Biology","doi":"10.1038/ncb3492","language":[{"iso":"eng"}],"intvolume":" 19","publication_identifier":{"issn":["14657392"]},"publist_id":"7074","author":[{"last_name":"Smutny","first_name":"Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","full_name":"Smutny, Michael"},{"last_name":"Ákos","first_name":"Zsuzsa","full_name":"Ákos, Zsuzsa"},{"last_name":"Grigolon","first_name":"Silvia","full_name":"Grigolon, Silvia"},{"full_name":"Shamipour, Shayan","last_name":"Shamipour","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Ruprecht, Verena","last_name":"Ruprecht","first_name":"Verena"},{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"Capek","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Behrndt","full_name":"Behrndt, Martin"},{"last_name":"Papusheva","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina","full_name":"Papusheva, Ekaterina"},{"full_name":"Tada, Masazumi","last_name":"Tada","first_name":"Masazumi"},{"last_name":"Hof","id":"3A374330-F248-11E8-B48F-1D18A9856A87","first_name":"Björn","orcid":"0000-0003-2057-2754","full_name":"Hof, Björn"},{"full_name":"Vicsek, Tamás","last_name":"Vicsek","first_name":"Tamás"},{"full_name":"Salbreux, Guillaume","first_name":"Guillaume","last_name":"Salbreux"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"citation":{"apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3492","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317.","ieee":"M. Smutny et al., “Friction forces position the neural anlage,” Nature Cell Biology, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” Nature Cell Biology. Nature Publishing Group, 2017. https://doi.org/10.1038/ncb3492.","mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” Nature Cell Biology, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:10.1038/ncb3492.","ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. Nature Cell Biology. 2017;19:306-317. doi:10.1038/ncb3492"},"type":"journal_article","volume":19,"status":"public","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"_id":"661","date_published":"2017-03-27T00:00:00Z","month":"03","year":"2017","date_updated":"2024-03-18T23:30:40Z","day":"27","related_material":{"record":[{"id":"50","relation":"dissertation_contains","status":"public"},{"status":"public","id":"8350","relation":"dissertation_contains"}]},"oa":1,"date_created":"2018-12-11T11:47:46Z","ec_funded":1,"main_file_link":[{"open_access":"1","url":"https://europepmc.org/articles/pmc5635970"}],"title":"Friction forces position the neural anlage","publisher":"Nature Publishing Group","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledged_ssus":[{"_id":"SSU"}],"oa_version":"Submitted Version","quality_controlled":"1","page":"306 - 317","abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"project":[{"call_identifier":"FP7","name":"Decoding the complexity of turbulence at its origin","_id":"25152F3A-B435-11E9-9278-68D0E5697425","grant_number":"306589"},{"name":"Control of Epithelial Cell Layer Spreading in Zebrafish","call_identifier":"FWF","_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I 930-B20"}],"pmid":1,"external_id":{"pmid":["28346437"]},"scopus_import":1},{"article_processing_charge":"No","page":"309-312","abstract":[{"lang":"eng","text":"The main goal of the SCP-ECG standard is to address ECG data and related metadata structuring, semantics and syntax, with the objective of facilitating interoperability and thus supporting and promoting the exchange of the relevant information for unary and serial ECG diagnosis. Starting with version V3.0, the standard now also provides support for the storage of continuous, long-term ECG recordings and affords a repository for selected ECG sequences and the related metadata to accommodate stress tests, drug trials and protocol-based ECG recordings. The global and per-lead measurements sections have been extended and three new sections have been introduced for storing beat-by-beat and/or spike-by-spike measurements\r\nand annotations. The used terminology and the provided measurements and annotations have been harmonized with the ISO/IEEE 11073-10102 Annotated ECG standard. Emphasis has also been put on harmonizing the Universal Statement Codes with the CDISC and the categorized AHA statement codes and similarly the drug and implanted devices codes with the ATC and NASPE/BPEG codes. "}],"scopus_import":"1","oa":1,"date_created":"2022-03-03T10:43:10Z","main_file_link":[{"url":"https://doi.org/10.22489/cinc.2016.090-500","open_access":"1"}],"oa_version":"Published Version","quality_controlled":"1","title":"SCP-ECG V3.0: An enhanced standard communication protocol for computer-assisted electrocardiography","publisher":"Computing in Cardiology","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CampIT"}],"_id":"10810","date_published":"2016-03-01T00:00:00Z","month":"03","date_updated":"2022-03-04T07:34:45Z","year":"2016","day":"01","intvolume":" 43","language":[{"iso":"eng"}],"publication_identifier":{"issn":["2325-887X"]},"publication":"2016 Computing in Cardiology Conference","doi":"10.22489/cinc.2016.090-500","acknowledgement":"The authors are thankful to Drs. Roger Abaecherli, Nikus Kjell, Paul Kligfield, Jay Mason, Patrice Nony, Vito Starc, Anders Thurin and the late Galen Wagner for their in depth review and constructive comments.","author":[{"first_name":"Paul","last_name":"Rubel","full_name":"Rubel, Paul"},{"full_name":"Pani, Danilo","last_name":"Pani","first_name":"Danilo"},{"last_name":"Schlögl","first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5621-8100","full_name":"Schlögl, Alois"},{"last_name":"Fayn","first_name":"Jocelyne","full_name":"Fayn, Jocelyne"},{"last_name":"Badilini","first_name":"Fabio","full_name":"Badilini, Fabio"},{"first_name":"Peter","last_name":"Macfarlane","full_name":"Macfarlane, Peter"},{"first_name":"Alpo","last_name":"Varri","full_name":"Varri, Alpo"}],"citation":{"ama":"Rubel P, Pani D, Schlögl A, et al. SCP-ECG V3.0: An enhanced standard communication protocol for computer-assisted electrocardiography. In: 2016 Computing in Cardiology Conference. Vol 43. Computing in Cardiology; 2016:309-312. doi:10.22489/cinc.2016.090-500","ista":"Rubel P, Pani D, Schlögl A, Fayn J, Badilini F, Macfarlane P, Varri A. 2016. SCP-ECG V3.0: An enhanced standard communication protocol for computer-assisted electrocardiography. 2016 Computing in Cardiology Conference. CinC: Computing in Cardiology vol. 43, 309–312.","chicago":"Rubel, Paul, Danilo Pani, Alois Schlögl, Jocelyne Fayn, Fabio Badilini, Peter Macfarlane, and Alpo Varri. “SCP-ECG V3.0: An Enhanced Standard Communication Protocol for Computer-Assisted Electrocardiography.” In 2016 Computing in Cardiology Conference, 43:309–12. Computing in Cardiology, 2016. https://doi.org/10.22489/cinc.2016.090-500.","mla":"Rubel, Paul, et al. “SCP-ECG V3.0: An Enhanced Standard Communication Protocol for Computer-Assisted Electrocardiography.” 2016 Computing in Cardiology Conference, vol. 43, Computing in Cardiology, 2016, pp. 309–12, doi:10.22489/cinc.2016.090-500.","ieee":"P. Rubel et al., “SCP-ECG V3.0: An enhanced standard communication protocol for computer-assisted electrocardiography,” in 2016 Computing in Cardiology Conference, Vancouver, Canada, 2016, vol. 43, pp. 309–312.","short":"P. Rubel, D. Pani, A. Schlögl, J. Fayn, F. Badilini, P. Macfarlane, A. Varri, in:, 2016 Computing in Cardiology Conference, Computing in Cardiology, 2016, pp. 309–312.","apa":"Rubel, P., Pani, D., Schlögl, A., Fayn, J., Badilini, F., Macfarlane, P., & Varri, A. (2016). SCP-ECG V3.0: An enhanced standard communication protocol for computer-assisted electrocardiography. In 2016 Computing in Cardiology Conference (Vol. 43, pp. 309–312). Vancouver, Canada: Computing in Cardiology. https://doi.org/10.22489/cinc.2016.090-500"},"conference":{"location":"Vancouver, Canada","start_date":"2016-09-11","name":"CinC: Computing in Cardiology","end_date":"2016-09-14"},"publication_status":"published","volume":43,"type":"conference","status":"public"},{"day":"07","date_updated":"2021-01-12T06:48:41Z","year":"2016","article_number":"36440","month":"11","date_published":"2016-11-07T00:00:00Z","_id":"1154","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"ToBo"}],"pubrep_id":"744","publication_status":"published","status":"public","volume":6,"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ama":"Schwarz J, Bierbaum V, Merrin J, et al. A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. Scientific Reports. 2016;6. doi:10.1038/srep36440","ista":"Schwarz J, Bierbaum V, Merrin J, Frank T, Hauschild R, Bollenbach MT, Tay S, Sixt MK, Mehling M. 2016. A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. Scientific Reports. 6, 36440.","chicago":"Schwarz, Jan, Veronika Bierbaum, Jack Merrin, Tino Frank, Robert Hauschild, Mark Tobias Bollenbach, Savaş Tay, Michael K Sixt, and Matthias Mehling. “A Microfluidic Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine Gradients.” Scientific Reports. Nature Publishing Group, 2016. https://doi.org/10.1038/srep36440.","mla":"Schwarz, Jan, et al. “A Microfluidic Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine Gradients.” Scientific Reports, vol. 6, 36440, Nature Publishing Group, 2016, doi:10.1038/srep36440.","ieee":"J. Schwarz et al., “A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients,” Scientific Reports, vol. 6. Nature Publishing Group, 2016.","apa":"Schwarz, J., Bierbaum, V., Merrin, J., Frank, T., Hauschild, R., Bollenbach, M. T., … Mehling, M. (2016). A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. Scientific Reports. Nature Publishing Group. https://doi.org/10.1038/srep36440","short":"J. Schwarz, V. Bierbaum, J. Merrin, T. Frank, R. Hauschild, M.T. Bollenbach, S. Tay, M.K. Sixt, M. Mehling, Scientific Reports 6 (2016)."},"author":[{"full_name":"Schwarz, Jan","last_name":"Schwarz","first_name":"Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Bierbaum, Veronika","last_name":"Bierbaum","first_name":"Veronika","id":"3FD04378-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin"},{"full_name":"Frank, Tino","last_name":"Frank","first_name":"Tino"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","first_name":"Mark Tobias","last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias","orcid":"0000-0003-4398-476X"},{"last_name":"Tay","first_name":"Savaş","full_name":"Tay, Savaş"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"},{"full_name":"Mehling, Matthias","orcid":"0000-0001-8599-1226","first_name":"Matthias","id":"3C23B994-F248-11E8-B48F-1D18A9856A87","last_name":"Mehling"}],"file_date_updated":"2018-12-12T10:09:32Z","publist_id":"6204","intvolume":" 6","language":[{"iso":"eng"}],"acknowledgement":"This work was supported by the Swiss National Science Foundation (Ambizione fellowship; PZ00P3-154733 to M.M.), the Swiss Multiple Sclerosis Society (research support to M.M.), a fellowship from the Boehringer Ingelheim Fonds (BIF) to J.S., the European Research Council (grant ERC GA 281556) and a START award from the Austrian Science Foundation (FWF) to M.S. #BioimagingFacility","doi":"10.1038/srep36440","publication":"Scientific Reports","scopus_import":1,"project":[{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)"},{"_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","grant_number":"Y 564-B12","call_identifier":"FWF","name":"Cytoskeletal force generation and transduction of leukocytes (FWF)"}],"file":[{"date_created":"2018-12-12T10:09:32Z","file_name":"IST-2017-744-v1+1_srep36440.pdf","file_id":"4756","content_type":"application/pdf","creator":"system","date_updated":"2018-12-12T10:09:32Z","relation":"main_file","access_level":"open_access","file_size":2353456}],"abstract":[{"lang":"eng","text":"Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue. \r\n"}],"has_accepted_license":"1","quality_controlled":"1","oa_version":"Published Version","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publisher":"Nature Publishing Group","title":"A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients","ec_funded":1,"date_created":"2018-12-11T11:50:27Z","oa":1,"ddc":["579"]},{"pubrep_id":"823","publication_status":"published","status":"public","type":"journal_article","volume":353,"citation":{"ieee":"J. Guzmán, A. Schlögl, M. Frotscher, and P. M. Jonas, “Synaptic mechanisms of pattern completion in the hippocampal CA3 network,” Science, vol. 353, no. 6304. American Association for the Advancement of Science, pp. 1117–1123, 2016.","short":"J. Guzmán, A. Schlögl, M. Frotscher, P.M. Jonas, Science 353 (2016) 1117–1123.","apa":"Guzmán, J., Schlögl, A., Frotscher, M., & Jonas, P. M. (2016). Synaptic mechanisms of pattern completion in the hippocampal CA3 network. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.aaf1836","ama":"Guzmán J, Schlögl A, Frotscher M, Jonas PM. Synaptic mechanisms of pattern completion in the hippocampal CA3 network. Science. 2016;353(6304):1117-1123. doi:10.1126/science.aaf1836","ista":"Guzmán J, Schlögl A, Frotscher M, Jonas PM. 2016. Synaptic mechanisms of pattern completion in the hippocampal CA3 network. Science. 353(6304), 1117–1123.","mla":"Guzmán, José, et al. “Synaptic Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” Science, vol. 353, no. 6304, American Association for the Advancement of Science, 2016, pp. 1117–23, doi:10.1126/science.aaf1836.","chicago":"Guzmán, José, Alois Schlögl, Michael Frotscher, and Peter M Jonas. “Synaptic Mechanisms of Pattern Completion in the Hippocampal CA3 Network.” Science. American Association for the Advancement of Science, 2016. https://doi.org/10.1126/science.aaf1836."},"author":[{"full_name":"Guzmán, José","id":"30CC5506-F248-11E8-B48F-1D18A9856A87","first_name":"José","last_name":"Guzmán"},{"first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"full_name":"Frotscher, Michael","first_name":"Michael","last_name":"Frotscher"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","last_name":"Jonas","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804"}],"file_date_updated":"2020-07-14T12:44:46Z","publist_id":"5899","intvolume":" 353","language":[{"iso":"eng"}],"doi":"10.1126/science.aaf1836","publication":"Science","day":"09","date_updated":"2021-01-12T06:50:04Z","year":"2016","month":"09","date_published":"2016-09-09T00:00:00Z","_id":"1350","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"oa_version":"Preprint","quality_controlled":"1","acknowledged_ssus":[{"_id":"ScienComp"}],"publisher":"American Association for the Advancement of Science","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Synaptic mechanisms of pattern completion in the hippocampal CA3 network","issue":"6304","ec_funded":1,"date_created":"2018-12-11T11:51:31Z","oa":1,"ddc":["570"],"scopus_import":1,"project":[{"_id":"25C0F108-B435-11E9-9278-68D0E5697425","grant_number":"268548","name":"Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons","call_identifier":"FP7"},{"grant_number":"P24909-B24","_id":"25C26B1E-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Mechanisms of transmitter release at GABAergic synapses"}],"abstract":[{"lang":"eng","text":"The hippocampal CA3 region plays a key role in learning and memory. Recurrent CA3–CA3\r\nsynapses are thought to be the subcellular substrate of pattern completion. However, the\r\nsynaptic mechanisms of this network computation remain enigmatic. To investigate these mechanisms, we combined functional connectivity analysis with network modeling.\r\nSimultaneous recording fromup to eight CA3 pyramidal neurons revealed that connectivity was sparse, spatially uniform, and highly enriched in disynaptic motifs (reciprocal, convergence,divergence, and chain motifs). Unitary connections were composed of one or two synaptic contacts, suggesting efficient use of postsynaptic space. Real-size modeling indicated that CA3 networks with sparse connectivity, disynaptic motifs, and single-contact connections robustly generated pattern completion.Thus, macro- and microconnectivity contribute to efficient\r\nmemory storage and retrieval in hippocampal networks."}],"has_accepted_license":"1","page":"1117 - 1123","file":[{"checksum":"89caefa4e181424cbf0aecc835fcc5ec","creator":"system","date_updated":"2020-07-14T12:44:46Z","relation":"main_file","access_level":"open_access","file_size":19408143,"file_name":"IST-2017-823-v1+1_aaf1836_CombinedPDF_v2-1.pdf","date_created":"2018-12-12T10:12:27Z","content_type":"application/pdf","file_id":"4945"}]},{"date_created":"2023-05-05T12:54:47Z","file_date_updated":"2023-05-16T07:03:56Z","author":[{"orcid":"0000-0002-5621-8100","full_name":"Schlögl, Alois","last_name":"Schlögl","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois"},{"last_name":"Stadlbauer","id":"4D0BC184-F248-11E8-B48F-1D18A9856A87","first_name":"Stephan","full_name":"Stadlbauer, Stephan"}],"publication":"AHPC16 - Austrian HPC Meeting 2016","ddc":["000"],"language":[{"iso":"eng"}],"oa":1,"title":"High performance computing at IST Austria: Modelling the human hippocampus","type":"conference_abstract","status":"public","publisher":"VSC - Vienna Scientific Cluster","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","conference":{"location":"Grundlsee, Austria","start_date":"2016-02-22","name":"AHPC: Austrian HPC Meeting","end_date":"2016-02-24"},"publication_status":"published","oa_version":"Published Version","quality_controlled":"1","main_file_link":[{"url":"https://vsc.ac.at/fileadmin/user_upload/vsc/conferences/ahpc16/BOOKLET_AHPC16.pdf","open_access":"1"}],"citation":{"mla":"Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at IST Austria: Modelling the Human Hippocampus.” AHPC16 - Austrian HPC Meeting 2016, VSC - Vienna Scientific Cluster, 2016, p. 37.","chicago":"Schlögl, Alois, and Stephan Stadlbauer. “High Performance Computing at IST Austria: Modelling the Human Hippocampus.” In AHPC16 - Austrian HPC Meeting 2016, 37. VSC - Vienna Scientific Cluster, 2016.","ama":"Schlögl A, Stadlbauer S. High performance computing at IST Austria: Modelling the human hippocampus. In: AHPC16 - Austrian HPC Meeting 2016. VSC - Vienna Scientific Cluster; 2016:37.","ista":"Schlögl A, Stadlbauer S. 2016. High performance computing at IST Austria: Modelling the human hippocampus. AHPC16 - Austrian HPC Meeting 2016. AHPC: Austrian HPC Meeting, 37.","apa":"Schlögl, A., & Stadlbauer, S. (2016). High performance computing at IST Austria: Modelling the human hippocampus. In AHPC16 - Austrian HPC Meeting 2016 (p. 37). Grundlsee, Austria: VSC - Vienna Scientific Cluster.","short":"A. Schlögl, S. Stadlbauer, in:, AHPC16 - Austrian HPC Meeting 2016, VSC - Vienna Scientific Cluster, 2016, p. 37.","ieee":"A. Schlögl and S. Stadlbauer, “High performance computing at IST Austria: Modelling the human hippocampus,” in AHPC16 - Austrian HPC Meeting 2016, Grundlsee, Austria, 2016, p. 37."},"date_published":"2016-02-24T00:00:00Z","month":"02","has_accepted_license":"1","page":"37","file":[{"relation":"main_file","access_level":"open_access","file_size":1073523,"creator":"dernst","checksum":"4a7b00362e81358d568f5e216fa03c3e","date_updated":"2023-05-16T07:03:56Z","content_type":"application/pdf","file_id":"12968","success":1,"date_created":"2023-05-16T07:03:56Z","file_name":"2016_AHPC_Schloegl.pdf"}],"article_processing_charge":"No","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"_id":"12903","day":"24","year":"2016","date_updated":"2023-05-16T07:15:14Z"},{"doi":"10.15479/AT:ISTA:44","oa":1,"ddc":["570"],"keyword":["cell migration","wide field microscopy","FIJI"],"date_created":"2018-12-12T12:31:31Z","author":[{"first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"}],"file_date_updated":"2020-07-14T12:47:02Z","citation":{"short":"R. Hauschild, (2016).","apa":"Hauschild, R. (2016). Fiji script to determine average speed and direction of migration of cells. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:44","ieee":"R. Hauschild, “Fiji script to determine average speed and direction of migration of cells.” Institute of Science and Technology Austria, 2016.","chicago":"Hauschild, Robert. “Fiji Script to Determine Average Speed and Direction of Migration of Cells.” Institute of Science and Technology Austria, 2016. https://doi.org/10.15479/AT:ISTA:44.","mla":"Hauschild, Robert. Fiji Script to Determine Average Speed and Direction of Migration of Cells. Institute of Science and Technology Austria, 2016, doi:10.15479/AT:ISTA:44.","ista":"Hauschild R. 2016. Fiji script to determine average speed and direction of migration of cells, Institute of Science and Technology Austria, 10.15479/AT:ISTA:44.","ama":"Hauschild R. Fiji script to determine average speed and direction of migration of cells. 2016. doi:10.15479/AT:ISTA:44"},"tmp":{"legal_code_url":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","image":"/images/cc_0.png","name":"Creative Commons Public Domain Dedication (CC0 1.0)","short":"CC0 (1.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Institute of Science and Technology Austria","status":"public","type":"research_data","title":"Fiji script to determine average speed and direction of migration of cells","oa_version":"Published Version","has_accepted_license":"1","file":[{"creator":"system","checksum":"9f96cddbcd4ed689f48712ffe234d5e5","date_updated":"2020-07-14T12:47:02Z","access_level":"open_access","relation":"main_file","file_size":20692,"file_name":"IST-2016-44-v1+1_migrationAnalyzer.zip","date_created":"2018-12-12T13:03:03Z","content_type":"application/zip","file_id":"5621"}],"abstract":[{"text":"This FIJI script calculates the population average of the migration speed as a function of time of all cells from wide field microscopy movies.","lang":"eng"}],"_id":"5555","article_processing_charge":"No","department":[{"_id":"Bio"}],"month":"07","date_published":"2016-07-08T00:00:00Z","datarep_id":"44","year":"2016","date_updated":"2024-02-21T13:50:06Z","day":"08"},{"publication":"Nature Cell Biology","doi":"10.1038/ncb3426","acknowledgement":"This work was supported by the German Research Foundation (DFG) Priority Program SP 1464 to T.E.B.S. and M.S., and European Research Council (ERC GA 281556) and Human Frontiers Program grants to M.S.\r\nService Units of IST Austria for excellent technical support.","intvolume":" 18","language":[{"iso":"eng"}],"publist_id":"5949","file_date_updated":"2020-07-14T12:44:43Z","author":[{"orcid":"0000-0002-1073-744X","full_name":"Leithner, Alexander F","last_name":"Leithner","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F"},{"id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander","last_name":"Eichner","full_name":"Eichner, Alexander"},{"full_name":"Müller, Jan","first_name":"Jan","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D","last_name":"Müller"},{"last_name":"Reversat","first_name":"Anne","id":"35B76592-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0666-8928","full_name":"Reversat, Anne"},{"first_name":"Markus","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","last_name":"Brown","full_name":"Brown, Markus"},{"full_name":"Schwarz, Jan","last_name":"Schwarz","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","first_name":"Jan"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin"},{"last_name":"De Gorter","first_name":"David","full_name":"De Gorter, David"},{"orcid":"0000-0003-4790-8078","full_name":"Schur, Florian","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian"},{"full_name":"Bayerl, Jonathan","first_name":"Jonathan","last_name":"Bayerl"},{"last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","full_name":"De Vries, Ingrid"},{"full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","last_name":"Wieser"},{"last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"full_name":"Lai, Frank","first_name":"Frank","last_name":"Lai"},{"last_name":"Moser","first_name":"Markus","full_name":"Moser, Markus"},{"full_name":"Kerjaschki, Dontscho","last_name":"Kerjaschki","first_name":"Dontscho"},{"last_name":"Rottner","first_name":"Klemens","full_name":"Rottner, Klemens"},{"full_name":"Small, Victor","first_name":"Victor","last_name":"Small"},{"full_name":"Stradal, Theresia","first_name":"Theresia","last_name":"Stradal"},{"last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"citation":{"mla":"Leithner, Alexander F., et al. “Diversified Actin Protrusions Promote Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” Nature Cell Biology, vol. 18, Nature Publishing Group, 2016, pp. 1253–59, doi:10.1038/ncb3426.","chicago":"Leithner, Alexander F, Alexander Eichner, Jan Müller, Anne Reversat, Markus Brown, Jan Schwarz, Jack Merrin, et al. “Diversified Actin Protrusions Promote Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” Nature Cell Biology. Nature Publishing Group, 2016. https://doi.org/10.1038/ncb3426.","ama":"Leithner AF, Eichner A, Müller J, et al. Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. Nature Cell Biology. 2016;18:1253-1259. doi:10.1038/ncb3426","ista":"Leithner AF, Eichner A, Müller J, Reversat A, Brown M, Schwarz J, Merrin J, De Gorter D, Schur FK, Bayerl J, de Vries I, Wieser S, Hauschild R, Lai F, Moser M, Kerjaschki D, Rottner K, Small V, Stradal T, Sixt MK. 2016. Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. Nature Cell Biology. 18, 1253–1259.","apa":"Leithner, A. F., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz, J., … Sixt, M. K. (2016). Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3426","short":"A.F. Leithner, A. Eichner, J. Müller, A. Reversat, M. Brown, J. Schwarz, J. Merrin, D. De Gorter, F.K. Schur, J. Bayerl, I. de Vries, S. Wieser, R. Hauschild, F. Lai, M. Moser, D. Kerjaschki, K. Rottner, V. Small, T. Stradal, M.K. Sixt, Nature Cell Biology 18 (2016) 1253–1259.","ieee":"A. F. Leithner et al., “Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes,” Nature Cell Biology, vol. 18. Nature Publishing Group, pp. 1253–1259, 2016."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)"},"volume":18,"type":"journal_article","status":"public","publication_status":"published","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"_id":"1321","date_published":"2016-10-24T00:00:00Z","month":"10","year":"2016","date_updated":"2024-03-18T23:30:16Z","day":"24","related_material":{"record":[{"relation":"dissertation_contains","id":"323","status":"public"}]},"article_type":"original","ddc":["570"],"oa":1,"date_created":"2018-12-11T11:51:21Z","ec_funded":1,"title":"Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publisher":"Nature Publishing Group","acknowledged_ssus":[{"_id":"SSU"}],"quality_controlled":"1","oa_version":"Submitted Version","has_accepted_license":"1","page":"1253 - 1259","file":[{"content_type":"application/pdf","file_id":"7844","date_created":"2020-05-14T16:33:46Z","file_name":"2018_NatureCell_Leithner.pdf","relation":"main_file","access_level":"open_access","file_size":4433280,"checksum":"e1411cb7c99a2d9089c178a6abef25e7","creator":"dernst","date_updated":"2020-07-14T12:44:43Z"}],"abstract":[{"lang":"eng","text":"Most migrating cells extrude their front by the force of actin polymerization. Polymerization requires an initial nucleation step, which is mediated by factors establishing either parallel filaments in the case of filopodia or branched filaments that form the branched lamellipodial network. Branches are considered essential for regular cell motility and are initiated by the Arp2/3 complex, which in turn is activated by nucleation-promoting factors of the WASP and WAVE families. Here we employed rapid amoeboid crawling leukocytes and found that deletion of the WAVE complex eliminated actin branching and thus lamellipodia formation. The cells were left with parallel filaments at the leading edge, which translated, depending on the differentiation status of the cell, into a unipolar pointed cell shape or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased speed and enormous directional persistence, while they were unable to turn towards chemotactic gradients. Cells with multiple filopodia retained chemotactic activity but their migration was progressively impaired with increasing geometrical complexity of the extracellular environment. These findings establish that diversified leading edge protrusions serve as explorative structures while they slow down actual locomotion."}],"article_processing_charge":"No","project":[{"call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556"}],"license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","scopus_import":1},{"date_created":"2018-12-11T11:52:31Z","oa":1,"ddc":["020"],"article_type":"original","publisher":"Verein Österreichischer Bibliothekare","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Arbeitsgruppe „Nationale Strategie“ des Open Access Network Austria OANA","quality_controlled":"1","oa_version":"Published Version","issue":"3","abstract":[{"lang":"eng","text":"Based on 16 recommendations, efforts should be made to achieve the following goal: By 2025, all scholarly publication activity in Austria should be Open Access. In other words, the final versions of all scholarly publications resulting from the support of public resources must be freely accessible on the Internet without delay (Gold Open Access). The resources required to meet this obligation shall be provided to the authors, or the cost of the publication venues shall be borne directly by the research organisations."}],"page":"580 - 607","has_accepted_license":"1","file":[{"content_type":"application/pdf","file_id":"5317","date_created":"2018-12-12T10:17:59Z","file_name":"IST-2016-720-v1+1_OANA_OA-Empfehlungen_12-11-2015.pdf","relation":"main_file","access_level":"open_access","file_size":931707,"creator":"system","checksum":"a495fe253bbc7615b1d60e9e85c94408","date_updated":"2020-07-14T12:45:00Z"}],"article_processing_charge":"No","scopus_import":1,"publist_id":"5648","author":[{"full_name":"Bauer, Bruno","first_name":"Bruno","last_name":"Bauer"},{"first_name":"Guido","last_name":"Blechl","full_name":"Blechl, Guido"},{"full_name":"Bock, Christoph","last_name":"Bock","first_name":"Christoph"},{"orcid":"0000-0002-6026-4409","full_name":"Danowski, Patrick","last_name":"Danowski","id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","first_name":"Patrick"},{"first_name":"Andreas","last_name":"Ferus","full_name":"Ferus, Andreas"},{"last_name":"Graschopf","first_name":"Anton","full_name":"Graschopf, Anton"},{"full_name":"König, Thomas","last_name":"König","first_name":"Thomas"},{"last_name":"Mayer","first_name":"Katja","full_name":"Mayer, Katja"},{"full_name":"Reckling, Falk","last_name":"Reckling","first_name":"Falk"},{"full_name":"Rieck, Katharina","first_name":"Katharina","last_name":"Rieck"},{"first_name":"Peter","last_name":"Seitz","full_name":"Seitz, Peter"},{"full_name":"Stöger, Herwig","first_name":"Herwig","last_name":"Stöger"},{"full_name":"Welzig, Elvira","last_name":"Welzig","first_name":"Elvira"}],"file_date_updated":"2020-07-14T12:45:00Z","doi":"10.5281/zenodo.33178","publication":"VÖB Mitteilungen","intvolume":" 68","language":[{"iso":"eng"}],"status":"public","volume":68,"type":"journal_article","publication_status":"published","pubrep_id":"720","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ista":"Bauer B, Blechl G, Bock C, Danowski P, Ferus A, Graschopf A, König T, Mayer K, Reckling F, Rieck K, Seitz P, Stöger H, Welzig E. 2015. Arbeitsgruppe „Nationale Strategie“ des Open Access Network Austria OANA. VÖB Mitteilungen. 68(3), 580–607.","ama":"Bauer B, Blechl G, Bock C, et al. Arbeitsgruppe „Nationale Strategie“ des Open Access Network Austria OANA. VÖB Mitteilungen. 2015;68(3):580-607. doi:10.5281/zenodo.33178","chicago":"Bauer, Bruno, Guido Blechl, Christoph Bock, Patrick Danowski, Andreas Ferus, Anton Graschopf, Thomas König, et al. “Arbeitsgruppe „Nationale Strategie“ Des Open Access Network Austria OANA.” VÖB Mitteilungen. Verein Österreichischer Bibliothekare, 2015. https://doi.org/10.5281/zenodo.33178.","mla":"Bauer, Bruno, et al. “Arbeitsgruppe „Nationale Strategie“ Des Open Access Network Austria OANA.” VÖB Mitteilungen, vol. 68, no. 3, Verein Österreichischer Bibliothekare, 2015, pp. 580–607, doi:10.5281/zenodo.33178.","ieee":"B. Bauer et al., “Arbeitsgruppe „Nationale Strategie“ des Open Access Network Austria OANA,” VÖB Mitteilungen, vol. 68, no. 3. Verein Österreichischer Bibliothekare, pp. 580–607, 2015.","short":"B. Bauer, G. Blechl, C. Bock, P. Danowski, A. Ferus, A. Graschopf, T. König, K. Mayer, F. Reckling, K. Rieck, P. Seitz, H. Stöger, E. Welzig, VÖB Mitteilungen 68 (2015) 580–607.","apa":"Bauer, B., Blechl, G., Bock, C., Danowski, P., Ferus, A., Graschopf, A., … Welzig, E. (2015). Arbeitsgruppe „Nationale Strategie“ des Open Access Network Austria OANA. VÖB Mitteilungen. Verein Österreichischer Bibliothekare. https://doi.org/10.5281/zenodo.33178"},"month":"11","date_published":"2015-11-12T00:00:00Z","_id":"1525","department":[{"_id":"E-Lib"}],"day":"12","year":"2015","date_updated":"2021-01-12T06:51:22Z"},{"page":"5055 - 5065","abstract":[{"text":"The plant hormone auxin is a key regulator of plant growth and development. Auxin levels are sensed and interpreted by distinct receptor systems that activate a broad range of cellular responses. The Auxin-Binding Protein1 (ABP1) that has been identified based on its ability to bind auxin with high affinity is a prime candidate for the extracellular receptor responsible for mediating a range of auxin effects, in particular, the fast non-transcriptional ones. Contradictory genetic studies suggested prominent or no importance of ABP1 in many developmental processes. However, how crucial the role of auxin binding to ABP1 is for its functions has not been addressed. Here, we show that the auxin-binding pocket of ABP1 is essential for its gain-of-function cellular and developmental roles. In total, 16 different abp1 mutants were prepared that possessed substitutions in the metal core or in the hydrophobic amino acids of the auxin-binding pocket as well as neutral mutations. Their analysis revealed that an intact auxin-binding pocket is a prerequisite for ABP1 to activate downstream components of the ABP1 signalling pathway, such as Rho of Plants (ROPs) and to mediate the clathrin association with membranes for endocytosis regulation. In planta analyses demonstrated the importance of the auxin binding pocket for all known ABP1-mediated postembryonic developmental processes, including morphology of leaf epidermal cells, root growth and root meristem activity, and vascular tissue differentiation. Taken together, these findings suggest that auxin binding to ABP1 is central to its function, supporting the role of ABP1 as auxin receptor.","lang":"eng"}],"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants"}],"scopus_import":1,"ec_funded":1,"date_created":"2018-12-11T11:52:44Z","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Oxford University Press","title":"Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles","quality_controlled":"1","oa_version":"None","issue":"16","month":"08","date_published":"2015-08-01T00:00:00Z","_id":"1562","department":[{"_id":"JiFr"},{"_id":"EM-Fac"}],"day":"01","year":"2015","date_updated":"2023-02-23T10:04:26Z","publist_id":"5609","author":[{"first_name":"Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","full_name":"Grones, Peter"},{"id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","first_name":"Xu","last_name":"Chen","full_name":"Chen, Xu"},{"last_name":"Simon","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","first_name":"Sibu","orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","last_name":"Kaufmann","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter"},{"full_name":"De Rycke, Riet","last_name":"De Rycke","first_name":"Riet"},{"full_name":"Nodzyński, Tomasz","last_name":"Nodzyński","first_name":"Tomasz"},{"full_name":"Zažímalová, Eva","first_name":"Eva","last_name":"Zažímalová"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"acknowledgement":"This work was supported by ERC Independent Research grant (ERC-2011-StG- 20101109-PSDP to JF); the European Social Fund and the state budget of the Czech Republic [the project ‘Employment of Newly Graduated Doctors of Science for Scientific Excellence’ (CZ.1.07/2.3.00/30.0009) to TN]; the Czech Science Foundation (GACR) [project 13-40637S to JF].","doi":"10.1093/jxb/erv177","publication":"Journal of Experimental Botany","intvolume":" 66","language":[{"iso":"eng"}],"status":"public","volume":66,"type":"journal_article","publication_status":"published","citation":{"short":"P. Grones, X. Chen, S. Simon, W. Kaufmann, R. De Rycke, T. Nodzyński, E. Zažímalová, J. Friml, Journal of Experimental Botany 66 (2015) 5055–5065.","apa":"Grones, P., Chen, X., Simon, S., Kaufmann, W., De Rycke, R., Nodzyński, T., … Friml, J. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/erv177","ieee":"P. Grones et al., “Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles,” Journal of Experimental Botany, vol. 66, no. 16. Oxford University Press, pp. 5055–5065, 2015.","chicago":"Grones, Peter, Xu Chen, Sibu Simon, Walter Kaufmann, Riet De Rycke, Tomasz Nodzyński, Eva Zažímalová, and Jiří Friml. “Auxin-Binding Pocket of ABP1 Is Crucial for Its Gain-of-Function Cellular and Developmental Roles.” Journal of Experimental Botany. Oxford University Press, 2015. https://doi.org/10.1093/jxb/erv177.","mla":"Grones, Peter, et al. “Auxin-Binding Pocket of ABP1 Is Crucial for Its Gain-of-Function Cellular and Developmental Roles.” Journal of Experimental Botany, vol. 66, no. 16, Oxford University Press, 2015, pp. 5055–65, doi:10.1093/jxb/erv177.","ista":"Grones P, Chen X, Simon S, Kaufmann W, De Rycke R, Nodzyński T, Zažímalová E, Friml J. 2015. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. 66(16), 5055–5065.","ama":"Grones P, Chen X, Simon S, et al. Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. Journal of Experimental Botany. 2015;66(16):5055-5065. doi:10.1093/jxb/erv177"}},{"citation":{"ieee":"B. Schwamb et al., “FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors,” International Journal of Cancer, vol. 137, no. 6. Wiley, pp. 1318–1329, 2015.","short":"B. Schwamb, R. Pick, S. Fernández, K. Völp, J. Heering, V. Dötsch, S. Bösser, J. Jung, R. Beinoravičiute Kellner, J. Wesely, I. Zörnig, M. Hammerschmidt, M. Nowak, R. Penzel, K. Zatloukal, S. Joos, R. Rieker, A. Agaimy, S. Söder, K. Reid Lombardo, M. Kendrick, M. Bardsley, Y. Hayashi, D. Asuzu, S. Syed, T. Ördög, M. Zörnig, International Journal of Cancer 137 (2015) 1318–1329.","apa":"Schwamb, B., Pick, R., Fernández, S., Völp, K., Heering, J., Dötsch, V., … Zörnig, M. (2015). FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. International Journal of Cancer. Wiley. https://doi.org/10.1002/ijc.29498","ista":"Schwamb B, Pick R, Fernández S, Völp K, Heering J, Dötsch V, Bösser S, Jung J, Beinoravičiute Kellner R, Wesely J, Zörnig I, Hammerschmidt M, Nowak M, Penzel R, Zatloukal K, Joos S, Rieker R, Agaimy A, Söder S, Reid Lombardo K, Kendrick M, Bardsley M, Hayashi Y, Asuzu D, Syed S, Ördög T, Zörnig M. 2015. FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. International Journal of Cancer. 137(6), 1318–1329.","ama":"Schwamb B, Pick R, Fernández S, et al. FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors. International Journal of Cancer. 2015;137(6):1318-1329. doi:10.1002/ijc.29498","mla":"Schwamb, Bettina, et al. “FAM96A Is a Novel Pro-Apoptotic Tumor Suppressor in Gastrointestinal Stromal Tumors.” International Journal of Cancer, vol. 137, no. 6, Wiley, 2015, pp. 1318–29, doi:10.1002/ijc.29498.","chicago":"Schwamb, Bettina, Robert Pick, Sara Fernández, Kirsten Völp, Jan Heering, Volker Dötsch, Susanne Bösser, et al. “FAM96A Is a Novel Pro-Apoptotic Tumor Suppressor in Gastrointestinal Stromal Tumors.” International Journal of Cancer. Wiley, 2015. https://doi.org/10.1002/ijc.29498."},"status":"public","volume":137,"type":"journal_article","publication_status":"published","doi":"10.1002/ijc.29498","publication":"International Journal of Cancer","language":[{"iso":"eng"}],"intvolume":" 137","publist_id":"5253","author":[{"last_name":"Schwamb","first_name":"Bettina","full_name":"Schwamb, Bettina"},{"last_name":"Pick","first_name":"Robert","full_name":"Pick, Robert"},{"first_name":"Sara","last_name":"Fernández","full_name":"Fernández, Sara"},{"last_name":"Völp","first_name":"Kirsten","full_name":"Völp, Kirsten"},{"last_name":"Heering","first_name":"Jan","full_name":"Heering, Jan"},{"full_name":"Dötsch, Volker","first_name":"Volker","last_name":"Dötsch"},{"full_name":"Bösser, Susanne","last_name":"Bösser","first_name":"Susanne"},{"last_name":"Jung","first_name":"Jennifer","full_name":"Jung, Jennifer"},{"full_name":"Beinoravičiute Kellner, Rasa","first_name":"Rasa","last_name":"Beinoravičiute Kellner"},{"full_name":"Wesely, Josephine","first_name":"Josephine","last_name":"Wesely"},{"full_name":"Zörnig, Inka","last_name":"Zörnig","first_name":"Inka"},{"full_name":"Hammerschmidt, Matthias","last_name":"Hammerschmidt","first_name":"Matthias"},{"first_name":"Matthias","id":"30845DAA-F248-11E8-B48F-1D18A9856A87","last_name":"Nowak","full_name":"Nowak, Matthias"},{"last_name":"Penzel","first_name":"Roland","full_name":"Penzel, Roland"},{"full_name":"Zatloukal, Kurt","last_name":"Zatloukal","first_name":"Kurt"},{"last_name":"Joos","first_name":"Stefan","full_name":"Joos, Stefan"},{"first_name":"Ralf","last_name":"Rieker","full_name":"Rieker, Ralf"},{"last_name":"Agaimy","first_name":"Abbas","full_name":"Agaimy, Abbas"},{"last_name":"Söder","first_name":"Stephan","full_name":"Söder, Stephan"},{"first_name":"Kmarie","last_name":"Reid Lombardo","full_name":"Reid Lombardo, Kmarie"},{"first_name":"Michael","last_name":"Kendrick","full_name":"Kendrick, Michael"},{"full_name":"Bardsley, Michael","first_name":"Michael","last_name":"Bardsley"},{"full_name":"Hayashi, Yujiro","last_name":"Hayashi","first_name":"Yujiro"},{"full_name":"Asuzu, David","last_name":"Asuzu","first_name":"David"},{"full_name":"Syed, Sabriya","first_name":"Sabriya","last_name":"Syed"},{"full_name":"Ördög, Tamás","last_name":"Ördög","first_name":"Tamás"},{"last_name":"Zörnig","first_name":"Martin","full_name":"Zörnig, Martin"}],"year":"2015","date_updated":"2021-01-12T06:53:36Z","day":"01","_id":"1848","department":[{"_id":"LifeSc"}],"month":"09","date_published":"2015-09-01T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497860/"}],"issue":"6","publisher":"Wiley","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"FAM96A is a novel pro-apoptotic tumor suppressor in gastrointestinal stromal tumors","quality_controlled":"1","oa_version":"Submitted Version","oa":1,"article_type":"original","date_created":"2018-12-11T11:54:20Z","external_id":{"pmid":["25716227"]},"pmid":1,"scopus_import":1,"abstract":[{"text":"The ability to escape apoptosis is a hallmark of cancer-initiating cells and a key factor of resistance to oncolytic therapy. Here, we identify FAM96A as a ubiquitous, evolutionarily conserved apoptosome-activating protein and investigate its potential pro-apoptotic tumor suppressor function in gastrointestinal stromal tumors (GISTs). Interaction between FAM96A and apoptotic peptidase activating factor 1 (APAF1) was identified in yeast two-hybrid screen and further studied by deletion mutants, glutathione-S-transferase pull-down, co-immunoprecipitation and immunofluorescence. Effects of FAM96A overexpression and knock-down on apoptosis sensitivity were examined in cancer cells and zebrafish embryos. Expression of FAM96A in GISTs and histogenetically related cells including interstitial cells of Cajal (ICCs), “fibroblast-like cells” (FLCs) and ICC stem cells (ICC-SCs) was investigated by Northern blotting, reverse transcription—polymerase chain reaction, immunohistochemistry and Western immunoblotting. Tumorigenicity of GIST cells and transformed murine ICC-SCs stably transduced to re-express FAM96A was studied by xeno- and allografting into immunocompromised mice. FAM96A was found to bind APAF1 and to enhance the induction of mitochondrial apoptosis. FAM96A protein or mRNA was dramatically reduced or lost in 106 of 108 GIST samples representing three independent patient cohorts. Whereas ICCs, ICC-SCs and FLCs, the presumed normal counterparts of GIST, were found to robustly express FAM96A protein and mRNA, FAM96A expression was much reduced in tumorigenic ICC-SCs. Re-expression of FAM96A in GIST cells and transformed ICC-SCs increased apoptosis sensitivity and diminished tumorigenicity. Our data suggest FAM96A is a novel pro-apoptotic tumor suppressor that is lost during GIST tumorigenesis.","lang":"eng"}],"page":"1318 - 1329","article_processing_charge":"No"},{"scopus_import":1,"abstract":[{"lang":"eng","text":"High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes."}],"has_accepted_license":"1","file":[{"content_type":"application/pdf","file_id":"4842","date_created":"2018-12-12T10:10:51Z","file_name":"IST-2017-837-v1+1_ingles-prieto.pdf","file_size":1308364,"relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:45:12Z","creator":"system","checksum":"e9fb251dfcb7cd209b83f17867e61321"}],"page":"952 - 954","project":[{"_id":"25548C20-B435-11E9-9278-68D0E5697425","grant_number":"303564","call_identifier":"FP7","name":"Microbial Ion Channels for Synthetic Neurobiology"},{"_id":"255BFFFA-B435-11E9-9278-68D0E5697425","grant_number":"RGY0084/2012","name":"In situ real-time imaging of neurotransmitter signaling using designer optical sensors (HFSP Young Investigator)"},{"grant_number":"W1232-B24","_id":"255A6082-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","call_identifier":"FWF"}],"issue":"12","title":"Light-assisted small-molecule screening against protein kinases","publisher":"Nature Publishing Group","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","oa_version":"Submitted Version","ddc":["571"],"oa":1,"date_created":"2018-12-11T11:53:25Z","ec_funded":1,"year":"2015","date_updated":"2023-09-07T12:49:09Z","day":"12","related_material":{"record":[{"relation":"dissertation_contains","id":"418","status":"public"}]},"department":[{"_id":"HaJa"},{"_id":"LifeSc"}],"_id":"1678","date_published":"2015-10-12T00:00:00Z","month":"10","citation":{"ieee":"Á. Inglés Prieto et al., “Light-assisted small-molecule screening against protein kinases,” Nature Chemical Biology, vol. 11, no. 12. Nature Publishing Group, pp. 952–954, 2015.","short":"Á. Inglés Prieto, E. Gschaider-Reichhart, M. Muellner, M. Nowak, S. Nijman, M. Grusch, H.L. Janovjak, Nature Chemical Biology 11 (2015) 952–954.","apa":"Inglés Prieto, Á., Gschaider-Reichhart, E., Muellner, M., Nowak, M., Nijman, S., Grusch, M., & Janovjak, H. L. (2015). Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. Nature Publishing Group. https://doi.org/10.1038/nchembio.1933","ista":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, Nowak M, Nijman S, Grusch M, Janovjak HL. 2015. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 11(12), 952–954.","ama":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, et al. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 2015;11(12):952-954. doi:10.1038/nchembio.1933","mla":"Inglés Prieto, Álvaro, et al. “Light-Assisted Small-Molecule Screening against Protein Kinases.” Nature Chemical Biology, vol. 11, no. 12, Nature Publishing Group, 2015, pp. 952–54, doi:10.1038/nchembio.1933.","chicago":"Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Markus Muellner, Matthias Nowak, Sebastian Nijman, Michael Grusch, and Harald L Janovjak. “Light-Assisted Small-Molecule Screening against Protein Kinases.” Nature Chemical Biology. Nature Publishing Group, 2015. https://doi.org/10.1038/nchembio.1933."},"type":"journal_article","volume":11,"status":"public","pubrep_id":"837","publication_status":"published","publication":"Nature Chemical Biology","acknowledgement":"This work was supported by grants from the European Union Seventh Framework Programme (CIG-303564 to H.J. and ERC-StG-311166 to S.M.B.N.), the Human Frontier Science Program (RGY0084_2012 to H.J.) and the Herzfelder Foundation (to M.G.). A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by the graduate program MolecularDrugTargets (Austrian Science Fund (FWF): W 1232) and a FemTech fellowship (3580812 Austrian Research Promotion Agency).","doi":"10.1038/nchembio.1933","language":[{"iso":"eng"}],"intvolume":" 11","publist_id":"5471","file_date_updated":"2020-07-14T12:45:12Z","author":[{"first_name":"Álvaro","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","last_name":"Inglés Prieto","full_name":"Inglés Prieto, Álvaro","orcid":"0000-0002-5409-8571"},{"last_name":"Gschaider-Reichhart","id":"3FEE232A-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-7218-7738","full_name":"Gschaider-Reichhart, Eva"},{"full_name":"Muellner, Markus","first_name":"Markus","last_name":"Muellner"},{"last_name":"Nowak","id":"30845DAA-F248-11E8-B48F-1D18A9856A87","first_name":"Matthias","full_name":"Nowak, Matthias"},{"full_name":"Nijman, Sebastian","last_name":"Nijman","first_name":"Sebastian"},{"first_name":"Michael","last_name":"Grusch","full_name":"Grusch, Michael"},{"orcid":"0000-0002-8023-9315","full_name":"Janovjak, Harald L","last_name":"Janovjak","first_name":"Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87"}]},{"title":"Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules","publisher":"Nature Publishing Group","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","oa_version":"Submitted Version","issue":"729","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4257754/","open_access":"1"}],"date_created":"2018-12-11T11:54:25Z","ec_funded":1,"article_type":"original","oa":1,"scopus_import":"1","pmid":1,"external_id":{"pmid":["25409144"]},"page":"90 - 93","abstract":[{"lang":"eng","text":"The prominent and evolutionarily ancient role of the plant hormone auxin is the regulation of cell expansion. Cell expansion requires ordered arrangement of the cytoskeleton but molecular mechanisms underlying its regulation by signalling molecules including auxin are unknown. Here we show in the model plant Arabidopsis thaliana that in elongating cells exogenous application of auxin or redistribution of endogenous auxin induces very rapid microtubule re-orientation from transverse to longitudinal, coherent with the inhibition of cell expansion. This fast auxin effect requires auxin binding protein 1 (ABP1) and involves a contribution of downstream signalling components such as ROP6 GTPase, ROP-interactive protein RIC1 and the microtubule-severing protein katanin. These components are required for rapid auxin-and ABP1-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses."}],"article_processing_charge":"No","project":[{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"type":"journal_article","volume":516,"status":"public","publication_status":"published","citation":{"ista":"Chen X, Grandont L, Li H, Hauschild R, Paque S, Abuzeineh A, Rakusova H, Benková E, Perrot Rechenmann C, Friml J. 2014. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. 516(729), 90–93.","ama":"Chen X, Grandont L, Li H, et al. Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. 2014;516(729):90-93. doi:10.1038/nature13889","mla":"Chen, Xu, et al. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” Nature, vol. 516, no. 729, Nature Publishing Group, 2014, pp. 90–93, doi:10.1038/nature13889.","chicago":"Chen, Xu, Laurie Grandont, Hongjiang Li, Robert Hauschild, Sébastien Paque, Anas Abuzeineh, Hana Rakusova, Eva Benková, Catherine Perrot Rechenmann, and Jiří Friml. “Inhibition of Cell Expansion by Rapid ABP1-Mediated Auxin Effect on Microtubules.” Nature. Nature Publishing Group, 2014. https://doi.org/10.1038/nature13889.","ieee":"X. Chen et al., “Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules,” Nature, vol. 516, no. 729. Nature Publishing Group, pp. 90–93, 2014.","apa":"Chen, X., Grandont, L., Li, H., Hauschild, R., Paque, S., Abuzeineh, A., … Friml, J. (2014). Inhibition of cell expansion by rapid ABP1-mediated auxin effect on microtubules. Nature. Nature Publishing Group. https://doi.org/10.1038/nature13889","short":"X. Chen, L. Grandont, H. Li, R. Hauschild, S. Paque, A. Abuzeineh, H. Rakusova, E. Benková, C. Perrot Rechenmann, J. Friml, Nature 516 (2014) 90–93."},"publist_id":"5237","author":[{"last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","first_name":"Xu","full_name":"Chen, Xu"},{"last_name":"Grandont","first_name":"Laurie","full_name":"Grandont, Laurie"},{"first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","last_name":"Li","full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"first_name":"Sébastien","last_name":"Paque","full_name":"Paque, Sébastien"},{"full_name":"Abuzeineh, Anas","first_name":"Anas","last_name":"Abuzeineh"},{"full_name":"Rakusova, Hana","first_name":"Hana","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA","last_name":"Rakusova"},{"last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"},{"first_name":"Catherine","last_name":"Perrot Rechenmann","full_name":"Perrot Rechenmann, Catherine"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"publication":"Nature","doi":"10.1038/nature13889","acknowledgement":"We thank R. Dixit for performing complementary experiments, D. W. Ehrhardt and T. Hashimoto for providing the seeds of TUB6–RFP and EB1b–GFP respectively, E. Zazimalova, J. Petrasek and M. Fendrych for discussing the manuscript and J. Leung for text optimization. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, to J.F.), ANR blanc AuxiWall project (ANR-11-BSV5-0007, to C.P.-R. and L.G.) and the Agency for Innovation by Science and Technology (IWT) (to H.R.). This work benefited from the facilities and expertise of the Imagif Cell Biology platform (http://www.imagif.cnrs.fr), which is supported by the Conseil Général de l’Essonne.","language":[{"iso":"eng"}],"intvolume":" 516","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"day":"04","year":"2014","date_updated":"2022-05-23T08:26:44Z","date_published":"2014-12-04T00:00:00Z","month":"12","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"EvBe"}],"_id":"1862"},{"date_created":"2018-12-11T11:54:34Z","ddc":["000"],"oa":1,"oa_version":"Published Version","title":"Sequential effects in continued visual search: Using fixation-related potentials to compare distractor processing before and after target detection","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publisher":"Wiley-Blackwell","issue":"4","abstract":[{"text":"To search for a target in a complex environment is an everyday behavior that ends with finding the target. When we search for two identical targets, however, we must continue the search after finding the first target and memorize its location. We used fixation-related potentials to investigate the neural correlates of different stages of the search, that is, before and after finding the first target. Having found the first target influenced subsequent distractor processing. Compared to distractor fixations before the first target fixation, a negative shift was observed for three subsequent distractor fixations. These results suggest that processing a target in continued search modulates the brain's response, either transiently by reflecting temporary working memory processes or permanently by reflecting working memory retention.","lang":"eng"}],"page":"385 - 395","has_accepted_license":"1","file":[{"date_created":"2018-12-12T10:16:44Z","file_name":"IST-2016-442-v1+1_K-rner_et_al-2014-Psychophysiology.pdf","content_type":"application/pdf","file_id":"5233","creator":"system","checksum":"4255b6185e774acce1d99f8e195c564d","date_updated":"2020-07-14T12:45:20Z","access_level":"open_access","relation":"main_file","file_size":543243}],"scopus_import":1,"file_date_updated":"2020-07-14T12:45:20Z","author":[{"last_name":"Körner","first_name":"Christof","full_name":"Körner, Christof"},{"last_name":"Braunstein","first_name":"Verena","full_name":"Braunstein, Verena"},{"first_name":"Matthias","last_name":"Stangl","full_name":"Stangl, Matthias"},{"first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"first_name":"Christa","last_name":"Neuper","full_name":"Neuper, Christa"},{"full_name":"Ischebeck, Anja","last_name":"Ischebeck","first_name":"Anja"}],"publist_id":"5205","language":[{"iso":"eng"}],"intvolume":" 51","publication":"Psychophysiology","acknowledgement":"Funded by Austrian Science Fund (FWF) Grant Number: P 22189-B18; European Union within the 6th Framework Programme Grant Number: 517590; State government of Styria Grant Number: PN 4055","doi":"10.1111/psyp.12062","publication_status":"published","pubrep_id":"442","volume":51,"type":"journal_article","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"C. Körner, V. Braunstein, M. Stangl, A. Schlögl, C. Neuper, and A. Ischebeck, “Sequential effects in continued visual search: Using fixation-related potentials to compare distractor processing before and after target detection,” Psychophysiology, vol. 51, no. 4. Wiley-Blackwell, pp. 385–395, 2014.","apa":"Körner, C., Braunstein, V., Stangl, M., Schlögl, A., Neuper, C., & Ischebeck, A. (2014). Sequential effects in continued visual search: Using fixation-related potentials to compare distractor processing before and after target detection. Psychophysiology. Wiley-Blackwell. https://doi.org/10.1111/psyp.12062","short":"C. Körner, V. Braunstein, M. Stangl, A. Schlögl, C. Neuper, A. Ischebeck, Psychophysiology 51 (2014) 385–395.","ama":"Körner C, Braunstein V, Stangl M, Schlögl A, Neuper C, Ischebeck A. Sequential effects in continued visual search: Using fixation-related potentials to compare distractor processing before and after target detection. Psychophysiology. 2014;51(4):385-395. doi:10.1111/psyp.12062","ista":"Körner C, Braunstein V, Stangl M, Schlögl A, Neuper C, Ischebeck A. 2014. Sequential effects in continued visual search: Using fixation-related potentials to compare distractor processing before and after target detection. Psychophysiology. 51(4), 385–395.","chicago":"Körner, Christof, Verena Braunstein, Matthias Stangl, Alois Schlögl, Christa Neuper, and Anja Ischebeck. “Sequential Effects in Continued Visual Search: Using Fixation-Related Potentials to Compare Distractor Processing before and after Target Detection.” Psychophysiology. Wiley-Blackwell, 2014. https://doi.org/10.1111/psyp.12062.","mla":"Körner, Christof, et al. “Sequential Effects in Continued Visual Search: Using Fixation-Related Potentials to Compare Distractor Processing before and after Target Detection.” Psychophysiology, vol. 51, no. 4, Wiley-Blackwell, 2014, pp. 385–95, doi:10.1111/psyp.12062."},"date_published":"2014-02-11T00:00:00Z","month":"02","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"_id":"1890","day":"11","date_updated":"2021-01-12T06:53:52Z","year":"2014"},{"language":[{"iso":"eng"}],"intvolume":" 281","publication":"Proceedings of the Royal Society of London Series B Biological Sciences","acknowledgement":"This research was supported by grants of the Swiss National Science Foundation to M.T.\r\nWe thank Tetsu Sato for providing field samples, Olivier Goffinet for field assistance, Dolores Schütz for vital help in the field and with the manuscript, David Lank, Barbara Taborsky, Suzanne Alonzo and two anonymous referees for comments on earlier manuscript versions, and the Fisheries Department, Ministry of Agriculture and Livestock of Zambia, for permission and support.","doi":"10.1098/rspb.2014.0253","author":[{"full_name":"Ocana, Sabine","first_name":"Sabine","last_name":"Ocana"},{"last_name":"Meidl","first_name":"Patrick","id":"4709BCE6-F248-11E8-B48F-1D18A9856A87","full_name":"Meidl, Patrick"},{"full_name":"Bonfils, Danielle","first_name":"Danielle","last_name":"Bonfils"},{"last_name":"Taborsky","first_name":"Michael","full_name":"Taborsky, Michael"}],"publist_id":"5203","citation":{"ama":"Ocana S, Meidl P, Bonfils D, Taborsky M. Y-linked Mendelian inheritance of giant and dwarf male morphs in shell-brooding cichlids. Proceedings of the Royal Society of London Series B Biological Sciences. 2014;281(1794). doi:10.1098/rspb.2014.0253","ista":"Ocana S, Meidl P, Bonfils D, Taborsky M. 2014. Y-linked Mendelian inheritance of giant and dwarf male morphs in shell-brooding cichlids. Proceedings of the Royal Society of London Series B Biological Sciences. 281(1794), 20140253.","chicago":"Ocana, Sabine, Patrick Meidl, Danielle Bonfils, and Michael Taborsky. “Y-Linked Mendelian Inheritance of Giant and Dwarf Male Morphs in Shell-Brooding Cichlids.” Proceedings of the Royal Society of London Series B Biological Sciences. The Royal Society, 2014. https://doi.org/10.1098/rspb.2014.0253.","mla":"Ocana, Sabine, et al. “Y-Linked Mendelian Inheritance of Giant and Dwarf Male Morphs in Shell-Brooding Cichlids.” Proceedings of the Royal Society of London Series B Biological Sciences, vol. 281, no. 1794, 20140253, The Royal Society, 2014, doi:10.1098/rspb.2014.0253.","ieee":"S. Ocana, P. Meidl, D. Bonfils, and M. Taborsky, “Y-linked Mendelian inheritance of giant and dwarf male morphs in shell-brooding cichlids,” Proceedings of the Royal Society of London Series B Biological Sciences, vol. 281, no. 1794. The Royal Society, 2014.","apa":"Ocana, S., Meidl, P., Bonfils, D., & Taborsky, M. (2014). Y-linked Mendelian inheritance of giant and dwarf male morphs in shell-brooding cichlids. Proceedings of the Royal Society of London Series B Biological Sciences. The Royal Society. https://doi.org/10.1098/rspb.2014.0253","short":"S. Ocana, P. Meidl, D. Bonfils, M. Taborsky, Proceedings of the Royal Society of London Series B Biological Sciences 281 (2014)."},"publication_status":"published","type":"journal_article","volume":281,"status":"public","department":[{"_id":"CampIT"}],"_id":"1892","article_number":"20140253","date_published":"2014-11-07T00:00:00Z","month":"11","date_updated":"2022-06-07T09:12:32Z","year":"2014","day":"07","article_type":"original","oa":1,"date_created":"2018-12-11T11:54:34Z","issue":"1794","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211437/"}],"oa_version":"Submitted Version","quality_controlled":"1","title":"Y-linked Mendelian inheritance of giant and dwarf male morphs in shell-brooding cichlids","publisher":"The Royal Society","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","abstract":[{"text":"Behavioural variation among conspecifics is typically contingent on individual state or environmental conditions. Sex-specific genetic polymorphisms are enigmatic because they lack conditionality, and genes causing adaptive trait variation in one sex may reduce Darwinian fitness in the other. One way to avoid such genetic antagonism is to control sex-specific traits by inheritance via sex chromosomes. Here, controlled laboratory crossings suggest that in snail-brooding cichlid fish a single locus, two-allele polymorphism located on a sex-linked chromosome of heterogametic males generates an extreme reproductive dimorphism. Both natural and sexual selection are responsible for exceptionally large body size of bourgeois males, creating a niche for a miniature male phenotype to evolve. This extreme intrasexual dimorphism results from selection on opposite size thresholds caused by a single ecological factor, empty snail shells used as breeding substrate. Paternity analyses reveal that in the field parasitic dwarf males sire the majority of offspring in direct sperm competition with large nest owners exceeding their size more than 40 times. Apparently, use of empty snail shells as breeding substrate and single locus sex-linked inheritance of growth are the major ecological and genetic mechanisms responsible for the extreme intrasexual diversity observed in Lamprologus callipterus.","lang":"eng"}],"pmid":1,"external_id":{"pmid":["25232141"]},"scopus_import":"1"},{"pubrep_id":"423","publication_status":"published","status":"public","volume":159,"type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"chicago":"Gao, Peng, Maria P Postiglione, Teresa Krieger, Luisirene Hernandez, Chao Wang, Zhi Han, Carmen Streicher, et al. “Deterministic Progenitor Behavior and Unitary Production of Neurons in the Neocortex.” Cell. Cell Press, 2014. https://doi.org/10.1016/j.cell.2014.10.027.","mla":"Gao, Peng, et al. “Deterministic Progenitor Behavior and Unitary Production of Neurons in the Neocortex.” Cell, vol. 159, no. 4, Cell Press, 2014, pp. 775–88, doi:10.1016/j.cell.2014.10.027.","ama":"Gao P, Postiglione MP, Krieger T, et al. Deterministic progenitor behavior and unitary production of neurons in the neocortex. Cell. 2014;159(4):775-788. doi:10.1016/j.cell.2014.10.027","ista":"Gao P, Postiglione MP, Krieger T, Hernandez L, Wang C, Han Z, Streicher C, Papusheva E, Insolera R, Chugh K, Kodish O, Huang K, Simons B, Luo L, Hippenmeyer S, Shi S. 2014. Deterministic progenitor behavior and unitary production of neurons in the neocortex. Cell. 159(4), 775–788.","short":"P. Gao, M.P. Postiglione, T. Krieger, L. Hernandez, C. Wang, Z. Han, C. Streicher, E. Papusheva, R. Insolera, K. Chugh, O. Kodish, K. Huang, B. Simons, L. Luo, S. Hippenmeyer, S. Shi, Cell 159 (2014) 775–788.","apa":"Gao, P., Postiglione, M. P., Krieger, T., Hernandez, L., Wang, C., Han, Z., … Shi, S. (2014). Deterministic progenitor behavior and unitary production of neurons in the neocortex. Cell. Cell Press. https://doi.org/10.1016/j.cell.2014.10.027","ieee":"P. Gao et al., “Deterministic progenitor behavior and unitary production of neurons in the neocortex,” Cell, vol. 159, no. 4. Cell Press, pp. 775–788, 2014."},"author":[{"full_name":"Gao, Peng","first_name":"Peng","last_name":"Gao"},{"first_name":"Maria P","id":"2C67902A-F248-11E8-B48F-1D18A9856A87","last_name":"Postiglione","full_name":"Postiglione, Maria P"},{"first_name":"Teresa","last_name":"Krieger","full_name":"Krieger, Teresa"},{"last_name":"Hernandez","first_name":"Luisirene","full_name":"Hernandez, Luisirene"},{"full_name":"Wang, Chao","last_name":"Wang","first_name":"Chao"},{"full_name":"Han, Zhi","first_name":"Zhi","last_name":"Han"},{"first_name":"Carmen","id":"36BCB99C-F248-11E8-B48F-1D18A9856A87","last_name":"Streicher","full_name":"Streicher, Carmen"},{"full_name":"Papusheva, Ekaterina","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina","last_name":"Papusheva"},{"first_name":"Ryan","last_name":"Insolera","full_name":"Insolera, Ryan"},{"last_name":"Chugh","first_name":"Kritika","full_name":"Chugh, Kritika"},{"last_name":"Kodish","first_name":"Oren","full_name":"Kodish, Oren"},{"full_name":"Huang, Kun","last_name":"Huang","first_name":"Kun"},{"last_name":"Simons","first_name":"Benjamin","full_name":"Simons, Benjamin"},{"full_name":"Luo, Liqun","first_name":"Liqun","last_name":"Luo"},{"last_name":"Hippenmeyer","first_name":"Simon","id":"37B36620-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2279-1061","full_name":"Hippenmeyer, Simon"},{"full_name":"Shi, Song","last_name":"Shi","first_name":"Song"}],"file_date_updated":"2020-07-14T12:45:25Z","publist_id":"5050","language":[{"iso":"eng"}],"intvolume":" 159","doi":"10.1016/j.cell.2014.10.027","publication":"Cell","day":"06","date_updated":"2021-01-12T06:54:47Z","year":"2014","month":"11","date_published":"2014-11-06T00:00:00Z","_id":"2022","department":[{"_id":"SiHi"},{"_id":"Bio"}],"oa_version":"Published Version","quality_controlled":"1","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publisher":"Cell Press","title":"Deterministic progenitor behavior and unitary production of neurons in the neocortex","issue":"4","ec_funded":1,"date_created":"2018-12-11T11:55:16Z","oa":1,"ddc":["570"],"scopus_import":1,"project":[{"_id":"25D61E48-B435-11E9-9278-68D0E5697425","grant_number":"618444","name":"Molecular Mechanisms of Cerebral Cortex Development","call_identifier":"FP7"},{"_id":"25D7962E-B435-11E9-9278-68D0E5697425","grant_number":"RGP0053/2014","name":"Quantitative Structure-Function Analysis of Cerebral Cortex Assembly at Clonal Level"}],"has_accepted_license":"1","page":"775 - 788","file":[{"creator":"system","checksum":"6c5de8329bb2ffa71cba9fda750f14ce","date_updated":"2020-07-14T12:45:25Z","access_level":"open_access","relation":"main_file","file_size":4435787,"file_id":"4709","content_type":"application/pdf","file_name":"IST-2016-423-v1+1_1-s2.0-S0092867414013154-main.pdf","date_created":"2018-12-12T10:08:47Z"}],"abstract":[{"text":"Radial glial progenitors (RGPs) are responsible for producing nearly all neocortical neurons. To gain insight into the patterns of RGP division and neuron production, we quantitatively analyzed excitatory neuron genesis in the mouse neocortex using Mosaic Analysis with Double Markers, which provides single-cell resolution of progenitor division patterns and potential in vivo. We found that RGPs progress through a coherent program in which their proliferative potential diminishes in a predictable manner. Upon entry into the neurogenic phase, individual RGPs produce ∼8–9 neurons distributed in both deep and superficial layers, indicating a unitary output in neuronal production. Removal of OTX1, a transcription factor transiently expressed in RGPs, results in both deep- and superficial-layer neuron loss and a reduction in neuronal unit size. Moreover, ∼1/6 of neurogenic RGPs proceed to produce glia. These results suggest that progenitor behavior and histogenesis in the mammalian neocortex conform to a remarkably orderly and deterministic program.","lang":"eng"}]},{"publication_identifier":{"issn":["16625196"]},"intvolume":" 8","language":[{"iso":"eng"}],"doi":"10.3389/fninf.2014.00016","publication":"Frontiers in Neuroinformatics","author":[{"first_name":"José","id":"30CC5506-F248-11E8-B48F-1D18A9856A87","last_name":"Guzmán","full_name":"Guzmán, José"},{"full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","first_name":"Alois","last_name":"Schlögl"},{"full_name":"Schmidt Hieber, Christoph","last_name":"Schmidt Hieber","first_name":"Christoph"}],"file_date_updated":"2020-07-14T12:45:34Z","publist_id":"4731","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ista":"Guzmán J, Schlögl A, Schmidt Hieber C. 2014. Stimfit: Quantifying electrophysiological data with Python. Frontiers in Neuroinformatics. 8(FEB), 16.","ama":"Guzmán J, Schlögl A, Schmidt Hieber C. Stimfit: Quantifying electrophysiological data with Python. Frontiers in Neuroinformatics. 2014;8(FEB). doi:10.3389/fninf.2014.00016","mla":"Guzmán, José, et al. “Stimfit: Quantifying Electrophysiological Data with Python.” Frontiers in Neuroinformatics, vol. 8, no. FEB, 16, Frontiers Research Foundation, 2014, doi:10.3389/fninf.2014.00016.","chicago":"Guzmán, José, Alois Schlögl, and Christoph Schmidt Hieber. “Stimfit: Quantifying Electrophysiological Data with Python.” Frontiers in Neuroinformatics. Frontiers Research Foundation, 2014. https://doi.org/10.3389/fninf.2014.00016.","ieee":"J. Guzmán, A. Schlögl, and C. Schmidt Hieber, “Stimfit: Quantifying electrophysiological data with Python,” Frontiers in Neuroinformatics, vol. 8, no. FEB. Frontiers Research Foundation, 2014.","short":"J. Guzmán, A. Schlögl, C. Schmidt Hieber, Frontiers in Neuroinformatics 8 (2014).","apa":"Guzmán, J., Schlögl, A., & Schmidt Hieber, C. (2014). Stimfit: Quantifying electrophysiological data with Python. Frontiers in Neuroinformatics. Frontiers Research Foundation. https://doi.org/10.3389/fninf.2014.00016"},"pubrep_id":"425","publication_status":"published","status":"public","type":"journal_article","volume":8,"_id":"2230","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"article_number":"16","month":"02","date_published":"2014-02-21T00:00:00Z","date_updated":"2021-01-12T06:56:09Z","year":"2014","day":"21","oa":1,"ddc":["570"],"date_created":"2018-12-11T11:56:27Z","issue":"FEB","quality_controlled":"1","oa_version":"Published Version","publisher":"Frontiers Research Foundation","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Stimfit: Quantifying electrophysiological data with Python","file":[{"content_type":"application/pdf","file_id":"4935","file_name":"IST-2016-425-v1+1_fninf-08-00016.pdf","date_created":"2018-12-12T10:12:17Z","file_size":2883372,"access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:45:34Z","checksum":"eeca00bba7232ff7d27db83321f6ea30","creator":"system"}],"abstract":[{"lang":"eng","text":"Intracellular electrophysiological recordings provide crucial insights into elementary neuronal signals such as action potentials and synaptic currents. Analyzing and interpreting these signals is essential for a quantitative understanding of neuronal information processing, and requires both fast data visualization and ready access to complex analysis routines. To achieve this goal, we have developed Stimfit, a free software package for cellular neurophysiology with a Python scripting interface and a built-in Python shell. The program supports most standard file formats for cellular neurophysiology and other biomedical signals through the Biosig library. To quantify and interpret the activity of single neurons and communication between neurons, the program includes algorithms to characterize the kinetics of presynaptic action potentials and postsynaptic currents, estimate latencies between pre- and postsynaptic events, and detect spontaneously occurring events. We validate and benchmark these algorithms, give estimation errors, and provide sample use cases, showing that Stimfit represents an efficient, accessible and extensible way to accurately analyze and interpret neuronal signals."}],"has_accepted_license":"1","scopus_import":1},{"department":[{"_id":"CampIT"}],"_id":"468","article_number":"0107518","date_published":"2014-09-23T00:00:00Z","month":"09","date_updated":"2021-01-12T08:00:48Z","year":"2014","day":"23","intvolume":" 9","language":[{"iso":"eng"}],"publication":"PLoS One","acknowledgement":"The study was funded by the University of Vienna (Focus of Excellence grant), the Galápagos Conservation Trust, and the Ethologische Gesellschaft e.V.","doi":"10.1371/journal.pone.0107518","file_date_updated":"2020-07-14T12:46:34Z","author":[{"full_name":"Cimadom, Arno","last_name":"Cimadom","first_name":"Arno"},{"last_name":"Ulloa","first_name":"Angel","full_name":"Ulloa, Angel"},{"full_name":"Meidl, Patrick","id":"4709BCE6-F248-11E8-B48F-1D18A9856A87","first_name":"Patrick","last_name":"Meidl"},{"first_name":"Markus","last_name":"Zöttl","full_name":"Zöttl, Markus"},{"full_name":"Zöttl, Elisabet","first_name":"Elisabet","last_name":"Zöttl"},{"last_name":"Fessl","first_name":"Birgit","full_name":"Fessl, Birgit"},{"full_name":"Nemeth, Erwin","last_name":"Nemeth","first_name":"Erwin"},{"full_name":"Dvorak, Michael","first_name":"Michael","last_name":"Dvorak"},{"full_name":"Cunninghame, Francesca","first_name":"Francesca","last_name":"Cunninghame"},{"last_name":"Tebbich","first_name":"Sabine","full_name":"Tebbich, Sabine"}],"publist_id":"7352","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ista":"Cimadom A, Ulloa A, Meidl P, Zöttl M, Zöttl E, Fessl B, Nemeth E, Dvorak M, Cunninghame F, Tebbich S. 2014. Invasive parasites habitat change and heavy rainfall reduce breeding success in Darwin’s finches. PLoS One. 9(9), 0107518.","ama":"Cimadom A, Ulloa A, Meidl P, et al. Invasive parasites habitat change and heavy rainfall reduce breeding success in Darwin’s finches. PLoS One. 2014;9(9). doi:10.1371/journal.pone.0107518","chicago":"Cimadom, Arno, Angel Ulloa, Patrick Meidl, Markus Zöttl, Elisabet Zöttl, Birgit Fessl, Erwin Nemeth, Michael Dvorak, Francesca Cunninghame, and Sabine Tebbich. “Invasive Parasites Habitat Change and Heavy Rainfall Reduce Breeding Success in Darwin’s Finches.” PLoS One. Public Library of Science, 2014. https://doi.org/10.1371/journal.pone.0107518.","mla":"Cimadom, Arno, et al. “Invasive Parasites Habitat Change and Heavy Rainfall Reduce Breeding Success in Darwin’s Finches.” PLoS One, vol. 9, no. 9, 0107518, Public Library of Science, 2014, doi:10.1371/journal.pone.0107518.","ieee":"A. Cimadom et al., “Invasive parasites habitat change and heavy rainfall reduce breeding success in Darwin’s finches,” PLoS One, vol. 9, no. 9. Public Library of Science, 2014.","short":"A. Cimadom, A. Ulloa, P. Meidl, M. Zöttl, E. Zöttl, B. Fessl, E. Nemeth, M. Dvorak, F. Cunninghame, S. Tebbich, PLoS One 9 (2014).","apa":"Cimadom, A., Ulloa, A., Meidl, P., Zöttl, M., Zöttl, E., Fessl, B., … Tebbich, S. (2014). Invasive parasites habitat change and heavy rainfall reduce breeding success in Darwin’s finches. PLoS One. Public Library of Science. https://doi.org/10.1371/journal.pone.0107518"},"publication_status":"published","pubrep_id":"954","volume":9,"type":"journal_article","status":"public","has_accepted_license":"1","file":[{"file_name":"IST-2018-954-v1+1_2014_Meidl_Invasive_parasites.PDF","date_created":"2018-12-12T10:14:48Z","content_type":"application/pdf","file_id":"5103","date_updated":"2020-07-14T12:46:34Z","checksum":"b24e7518ccd41effed0d7d9e2498f67f","creator":"system","file_size":489387,"relation":"main_file","access_level":"open_access"}],"abstract":[{"lang":"eng","text":"Invasive alien parasites and pathogens are a growing threat to biodiversity worldwide, which can contribute to the extinction of endemic species. On the Galápagos Islands, the invasive parasitic fly Philornis downsi poses a major threat to the endemic avifauna. Here, we investigated the influence of this parasite on the breeding success of two Darwin's finch species, the warbler finch (Certhidea olivacea) and the sympatric small tree finch (Camarhynchus parvulus), on Santa Cruz Island in 2010 and 2012. While the population of the small tree finch appeared to be stable, the warbler finch has experienced a dramatic decline in population size on Santa Cruz Island since 1997. We aimed to identify whether warbler finches are particularly vulnerable during different stages of the breeding cycle. Contrary to our prediction, breeding success was lower in the small tree finch than in the warbler finch. In both species P. downsi had a strong negative impact on breeding success and our data suggest that heavy rain events also lowered the fledging success. On the one hand parents might be less efficient in compensating their chicks' energy loss due to parasitism as they might be less efficient in foraging on days of heavy rain. On the other hand, intense rainfalls might lead to increased humidity and more rapid cooling of the nests. In the case of the warbler finch we found that the control of invasive plant species with herbicides had a significant additive negative impact on the breeding success. It is very likely that the availability of insects (i.e. food abundance) is lower in such controlled areas, as herbicide usage led to the removal of the entire understory. Predation seems to be a minor factor in brood loss."}],"scopus_import":1,"ddc":["576"],"oa":1,"date_created":"2018-12-11T11:46:38Z","issue":"9","quality_controlled":"1","oa_version":"Published Version","title":"Invasive parasites habitat change and heavy rainfall reduce breeding success in Darwin's finches","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publisher":"Public Library of Science"},{"date_published":"2014-01-01T00:00:00Z","department":[{"_id":"E-Lib"}],"_id":"5422","has_accepted_license":"1","file":[{"checksum":"3954896648ce8afa8f7c4425e71cff08","creator":"system","date_updated":"2020-07-14T12:46:50Z","access_level":"open_access","relation":"main_file","file_size":648585,"file_id":"5501","content_type":"application/pdf","date_created":"2018-12-12T11:53:40Z","file_name":"IST-2014-254-v1+1_Dublin_Day_3.pdf"},{"date_updated":"2020-07-14T12:46:50Z","creator":"system","checksum":"9a0d42b0b832dfe7e4b22fb6816bcbba","file_size":221339,"relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_id":"5502","file_name":"IST-2014-254-v1+2_Dublin_Day_1.pdf","date_created":"2018-12-12T11:53:41Z"},{"date_created":"2018-12-12T11:53:42Z","file_name":"IST-2014-254-v1+3_Dublin_Day_2.pdf","file_id":"5503","content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_size":187778,"creator":"system","checksum":"498b8d629fb1bd17bff1dc43700a93e6","date_updated":"2020-07-14T12:46:50Z"}],"abstract":[{"lang":"eng","text":"Notes from the Third Plenary for the Research Data Alliance in Dublin, Ireland on March 26 to 28, 2014 with focus on starting an institutional research data repository."}],"date_updated":"2020-07-14T23:04:56Z","year":"2014","file_date_updated":"2020-07-14T12:46:50Z","author":[{"first_name":"Jana","id":"3252EDC2-F248-11E8-B48F-1D18A9856A87","last_name":"Porsche","full_name":"Porsche, Jana"}],"date_created":"2018-12-12T11:39:14Z","language":[{"iso":"eng"}],"ddc":["020"],"oa":1,"oa_version":"None","pubrep_id":"254","title":"Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland","type":"report","publisher":"none","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","citation":{"ama":"Porsche J. Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland. none; 2014.","ista":"Porsche J. 2014. Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland, none,p.","mla":"Porsche, Jana. Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland. none, 2014.","chicago":"Porsche, Jana. Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland. none, 2014.","ieee":"J. Porsche, Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland. none, 2014.","short":"J. Porsche, Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland, none, 2014.","apa":"Porsche, J. (2014). Notes from Research Data Alliance Plenary Meeting in Dublin, Ireland. none."}},{"date_created":"2018-12-11T11:56:36Z","ddc":["020"],"oa":1,"title":"Linked (Open) Data - Bibliographische Daten im Semantic Web","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Verein Österreichischer Bibliothekarinnen und Bibliothekare","oa_version":"Published Version","issue":"3/4","popular_science":"1","page":"559 - 587","file":[{"file_name":"IST-2016-719-v1+1_Patrick_Danowski__Doron_Goldfarb__Verena_Schaffner__Wolfram_Seidler_Linked__Open__Data_Bibliographische_Daten_im_Semantic_Web.pdf","date_created":"2018-12-12T10:08:09Z","file_id":"4669","content_type":"application/pdf","access_level":"open_access","relation":"main_file","file_size":881545,"checksum":"ae57ffcee3720adcc27b0f2767a1e04b","creator":"system","date_updated":"2020-07-14T12:45:35Z"}],"has_accepted_license":"1","abstract":[{"lang":"eng","text":"Linked (Open) Data - bibliographic data on the Semantic Web. Report of the Working Group on Linked Data to the plenary assembly of the Austrian Library Network (translation of the title). Linked Data stands for a certain approach to publishing data on the Web. The underlying idea is to harmonise heterogeneous data sources of different origin in order to improve their accessibility and interoperability, effectively making them queryable as a big distributed database. This report summarises relevant developments in Europe as well as the Linked Data Working Group‘s strategic and technical considerations regarding the publishing of the Austrian Library Network’s (OBV’s) bibliographic datasets. It concludes with the mutual agreement that the implementation of Linked Data principles within the OBV can only be taken into consideration accompanied by a discussion about the provision of the datasets under a free license."},{"text":"Linked Data steht für eine bestimmte Form der Veröffentlichung von Daten via Internet. Die zu Grunde liegende Idee ist es, Daten verschiedenster Provenienz, die derzeit teilweise gar nicht oder nur schwer zugänglich sind, in möglichst \r\neinheitlicher Form miteinander zu verknüpfen und dadurch in ihrer Gesamtheit abfragbar zu machen.\r\nDieser Bericht fasst die Entwicklungen im europäischen Raum, sowie strategische und technische Überlegungen der AG Linked Data hinsichtlich der Veröffentlichung von bibliothekarischen Daten des Österreichischen Bibliothekenverbundes (OBV) zusammen und schließt mit der gemeinsamen Übereinkunft, dass die Umsetzung von Linked Data-Prinzipien im OBV nur in Zusammenhang mit einer Diskussion über die damit einhergehende Veröffentlichung der Daten unter einer freien Lizenz angedacht werden sollte.","lang":"ger"}],"publist_id":"4690","file_date_updated":"2020-07-14T12:45:35Z","author":[{"id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","first_name":"Patrick","last_name":"Danowski","full_name":"Danowski, Patrick","orcid":"0000-0002-6026-4409"},{"last_name":"Goldfarb","first_name":"Doron","full_name":"Goldfarb, Doron"},{"full_name":"Schaffner, Verena","first_name":"Verena","last_name":"Schaffner"},{"full_name":"Seidler, Wolfram","last_name":"Seidler","first_name":"Wolfram"}],"publication":"VÖB Mitteilungen","intvolume":" 66","language":[{"iso":"eng"}],"volume":66,"type":"journal_article","status":"public","publication_status":"published","pubrep_id":"719","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"P. Danowski, D. Goldfarb, V. Schaffner, and W. Seidler, “Linked (Open) Data - Bibliographische Daten im Semantic Web,” VÖB Mitteilungen, vol. 66, no. 3/4. Verein Österreichischer Bibliothekarinnen und Bibliothekare, pp. 559–587, 2013.","apa":"Danowski, P., Goldfarb, D., Schaffner, V., & Seidler, W. (2013). Linked (Open) Data - Bibliographische Daten im Semantic Web. VÖB Mitteilungen. Verein Österreichischer Bibliothekarinnen und Bibliothekare.","short":"P. Danowski, D. Goldfarb, V. Schaffner, W. Seidler, VÖB Mitteilungen 66 (2013) 559–587.","ama":"Danowski P, Goldfarb D, Schaffner V, Seidler W. Linked (Open) Data - Bibliographische Daten im Semantic Web. VÖB Mitteilungen. 2013;66(3/4):559-587.","ista":"Danowski P, Goldfarb D, Schaffner V, Seidler W. 2013. Linked (Open) Data - Bibliographische Daten im Semantic Web. VÖB Mitteilungen. 66(3/4), 559–587.","chicago":"Danowski, Patrick, Doron Goldfarb, Verena Schaffner, and Wolfram Seidler. “Linked (Open) Data - Bibliographische Daten Im Semantic Web.” VÖB Mitteilungen. Verein Österreichischer Bibliothekarinnen und Bibliothekare, 2013.","mla":"Danowski, Patrick, et al. “Linked (Open) Data - Bibliographische Daten Im Semantic Web.” VÖB Mitteilungen, vol. 66, no. 3/4, Verein Österreichischer Bibliothekarinnen und Bibliothekare, 2013, pp. 559–87."},"date_published":"2013-12-01T00:00:00Z","month":"12","department":[{"_id":"E-Lib"}],"_id":"2256","day":"01","year":"2013","date_updated":"2021-01-12T06:56:20Z"},{"publication_status":"published","pubrep_id":"725","status":"public","volume":50,"type":"book","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"P. Danowski and A. Pohl, (Open) Linked Data in Bibliotheken, vol. 50. De Gruyter, 2013.","apa":"Danowski, P., & Pohl, A. (2013). (Open) Linked Data in Bibliotheken (Vol. 50). De Gruyter. https://doi.org/10.1515/9783110278736","short":"P. Danowski, A. Pohl, (Open) Linked Data in Bibliotheken, De Gruyter, 2013.","ista":"Danowski P, Pohl A. 2013. (Open) Linked Data in Bibliotheken, De Gruyter,p.","ama":"Danowski P, Pohl A. (Open) Linked Data in Bibliotheken. Vol 50. De Gruyter; 2013. doi:10.1515/9783110278736","mla":"Danowski, Patrick, and Adrian Pohl. (Open) Linked Data in Bibliotheken. Vol. 50, De Gruyter, 2013, doi:10.1515/9783110278736.","chicago":"Danowski, Patrick, and Adrian Pohl. (Open) Linked Data in Bibliotheken. Vol. 50. De Gruyter, 2013. https://doi.org/10.1515/9783110278736."},"author":[{"last_name":"Danowski","first_name":"Patrick","id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6026-4409","full_name":"Danowski, Patrick"},{"last_name":"Pohl","first_name":"Adrian","full_name":"Pohl, Adrian"}],"file_date_updated":"2020-07-14T12:45:38Z","publist_id":"4621","publication_identifier":{"issn":["2191-3587"],"eisbn":["9-783-1102-7873-6"],"isbn":[" 978-3-11-027634-3"]},"intvolume":" 50","language":[{"iso":"ger"}],"doi":"10.1515/9783110278736","day":"13","date_updated":"2021-12-21T12:17:19Z","year":"2013","alternative_title":["Bibliotheks- und Informationspraxis"],"month":"09","date_published":"2013-09-13T00:00:00Z","_id":"2306","department":[{"_id":"E-Lib"}],"quality_controlled":"1","oa_version":"Published Version","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publisher":"De Gruyter","title":"(Open) Linked Data in 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Buch ist sowohl eine Einführung in die Themen Linked Data, Open Data und Open Linked Data als es auch den konkreten Bezug auf Bibliotheken behandelt. Hierzu werden konkrete Anwendungsprojekte beschrieben. Der Band wendet sich dabei sowohl an Personen aus der Bibliothekspraxis als auch an Personen aus dem Bibliotheksmanagement, die noch nicht mit dem Thema vertraut sind.","lang":"ger"}],"has_accepted_license":"1"},{"date_created":"2018-12-11T11:57:30Z","ddc":["576"],"oa":1,"quality_controlled":"1","oa_version":"Published Version","title":"Complete genome sequence of the novel phage MG-B1 infecting bacillus weihenstephanensis","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"American Society for Microbiology","issue":"3","abstract":[{"text":"Here, we describe a novel virulent bacteriophage that infects Bacillus weihenstephanensis, isolated from soil in Austria. It is the first phage to be discovered that infects this species. Here, we present the complete genome sequence of this podovirus. ","lang":"eng"}],"has_accepted_license":"1","file":[{"date_created":"2018-12-12T10:17:36Z","file_name":"IST-2015-398-v1+1_Genome_Announc.-2013-Redondo-.pdf","file_id":"5291","content_type":"application/pdf","creator":"system","checksum":"0751ec74b695567e0cdf02aaf9c26829","date_updated":"2020-07-14T12:45:40Z","relation":"main_file","access_level":"open_access","file_size":130026}],"scopus_import":1,"file_date_updated":"2020-07-14T12:45:40Z","author":[{"orcid":"0000-0002-5837-2793","full_name":"Fernandes Redondo, Rodrigo A","last_name":"Fernandes Redondo","first_name":"Rodrigo A","id":"409D5C96-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Kupczok, Anne","last_name":"Kupczok","first_name":"Anne","id":"2BB22BC2-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Stift","first_name":"Gertraud","id":"2DB195CA-F248-11E8-B48F-1D18A9856A87","full_name":"Stift, Gertraud"},{"full_name":"Bollback, Jonathan P","orcid":"0000-0002-4624-4612","id":"2C6FA9CC-F248-11E8-B48F-1D18A9856A87","first_name":"Jonathan P","last_name":"Bollback"}],"publist_id":"4516","intvolume":" 1","language":[{"iso":"eng"}],"publication":"Genome Announcements","doi":"10.1128/genomeA.00216-13","pubrep_id":"398","publication_status":"published","type":"journal_article","volume":1,"status":"public","citation":{"chicago":"Fernandes Redondo, Rodrigo A, Anne Kupczok, Gertraud Stift, and Jonathan P Bollback. “Complete Genome Sequence of the Novel Phage MG-B1 Infecting Bacillus Weihenstephanensis.” Genome Announcements. American Society for Microbiology, 2013. https://doi.org/10.1128/genomeA.00216-13.","mla":"Fernandes Redondo, Rodrigo A., et al. “Complete Genome Sequence of the Novel Phage MG-B1 Infecting Bacillus Weihenstephanensis.” Genome Announcements, vol. 1, no. 3, American Society for Microbiology, 2013, doi:10.1128/genomeA.00216-13.","ama":"Fernandes Redondo RA, Kupczok A, Stift G, Bollback JP. Complete genome sequence of the novel phage MG-B1 infecting bacillus weihenstephanensis. Genome Announcements. 2013;1(3). doi:10.1128/genomeA.00216-13","ista":"Fernandes Redondo RA, Kupczok A, Stift G, Bollback JP. 2013. Complete genome sequence of the novel phage MG-B1 infecting bacillus weihenstephanensis. Genome Announcements. 1(3).","apa":"Fernandes Redondo, R. A., Kupczok, A., Stift, G., & Bollback, J. P. (2013). Complete genome sequence of the novel phage MG-B1 infecting bacillus weihenstephanensis. Genome Announcements. American Society for Microbiology. https://doi.org/10.1128/genomeA.00216-13","short":"R.A. Fernandes Redondo, A. Kupczok, G. Stift, J.P. Bollback, Genome Announcements 1 (2013).","ieee":"R. A. Fernandes Redondo, A. Kupczok, G. Stift, and J. P. Bollback, “Complete genome sequence of the novel phage MG-B1 infecting bacillus weihenstephanensis,” Genome Announcements, vol. 1, no. 3. American Society for Microbiology, 2013."},"date_published":"2013-06-13T00:00:00Z","month":"06","department":[{"_id":"JoBo"},{"_id":"LifeSc"}],"_id":"2410","day":"13","date_updated":"2021-01-12T06:57:19Z","year":"2013"},{"date_published":"2013-01-18T00:00:00Z","month":"01","department":[{"_id":"MiSi"},{"_id":"Bio"}],"_id":"2839","day":"18","date_updated":"2022-06-10T10:21:40Z","year":"2013","author":[{"full_name":"Weber, Michele","last_name":"Weber","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","first_name":"Michele"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"full_name":"Schwarz, Jan","last_name":"Schwarz","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","first_name":"Jan"},{"full_name":"Moussion, Christine","last_name":"Moussion","first_name":"Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87"},{"last_name":"De Vries","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","full_name":"De Vries, Ingrid"},{"last_name":"Legler","first_name":"Daniel","full_name":"Legler, Daniel"},{"full_name":"Luther, Sanjiv","first_name":"Sanjiv","last_name":"Luther"},{"first_name":"Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias","orcid":"0000-0003-4398-476X"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"publist_id":"3959","intvolume":" 339","language":[{"iso":"eng"}],"publication":"Science","doi":"10.1126/science.1228456","acknowledgement":"We thank M. Frank for technical assistance and S. Cremer, P. Schmalhorst, and E. Kiermaier for critical reading of the manuscript. This work was supported by a Humboldt Foundation postdoctoral fellowship (to M.W.), the German Research Foundation (Si1323 1,2 to M.S.), the Human Frontier Science Program (HFSP RGP0058/2011 to M.S.), the European Research Council (ERC StG 281556 to M.S.), and the Swiss National Science Foundation (31003A 127474 to D.F.L., 130488 to S.A.L.).","publication_status":"published","type":"journal_article","volume":339,"status":"public","citation":{"ista":"Weber M, Hauschild R, Schwarz J, Moussion C, de Vries I, Legler D, Luther S, Bollenbach MT, Sixt MK. 2013. Interstitial dendritic cell guidance by haptotactic chemokine gradients. Science. 339(6117), 328–332.","ama":"Weber M, Hauschild R, Schwarz J, et al. Interstitial dendritic cell guidance by haptotactic chemokine gradients. Science. 2013;339(6117):328-332. doi:10.1126/science.1228456","chicago":"Weber, Michele, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid de Vries, Daniel Legler, Sanjiv Luther, Mark Tobias Bollenbach, and Michael K Sixt. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” Science. American Association for the Advancement of Science, 2013. https://doi.org/10.1126/science.1228456.","mla":"Weber, Michele, et al. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” Science, vol. 339, no. 6117, American Association for the Advancement of Science, 2013, pp. 328–32, doi:10.1126/science.1228456.","ieee":"M. Weber et al., “Interstitial dendritic cell guidance by haptotactic chemokine gradients,” Science, vol. 339, no. 6117. American Association for the Advancement of Science, pp. 328–332, 2013.","short":"M. Weber, R. Hauschild, J. Schwarz, C. Moussion, I. de Vries, D. Legler, S. Luther, M.T. Bollenbach, M.K. Sixt, Science 339 (2013) 328–332.","apa":"Weber, M., Hauschild, R., Schwarz, J., Moussion, C., de Vries, I., Legler, D., … Sixt, M. K. (2013). Interstitial dendritic cell guidance by haptotactic chemokine gradients. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1228456"},"article_processing_charge":"No","project":[{"call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556"},{"_id":"25ABD200-B435-11E9-9278-68D0E5697425","grant_number":"RGP0058/2011","name":"Cell migration in complex environments: from in vivo experiments to theoretical models"}],"page":"328 - 332","abstract":[{"text":"Directional guidance of cells via gradients of chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous gradients of the chemokine CCL21 within mouse skin and show that they guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots of CCL21 within lymphatic endothelial cells and steeply decaying gradients within the perilymphatic interstitium. These gradients match the migratory patterns of the dendritic cells, which directionally approach vessels from a distance of up to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and its experimental delocalization or swamping the endogenous gradients abolishes directed migration. These findings functionally establish the concept of haptotaxis, directed migration along immobilized gradients, in tissues.","lang":"eng"}],"scopus_import":"1","date_created":"2018-12-11T11:59:52Z","ec_funded":1,"article_type":"original","oa":1,"quality_controlled":"1","oa_version":"Published Version","title":"Interstitial dendritic cell guidance by haptotactic chemokine gradients","publisher":"American Association for the Advancement of Science","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"6117","main_file_link":[{"url":"https://kops.uni-konstanz.de/bitstream/123456789/26341/2/Weber_263418.pdf","open_access":"1"}]},{"has_accepted_license":"1","file":[{"file_size":151208,"access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:46:45Z","creator":"system","checksum":"d68712db838432ecdacf9ffb1de8f8a6","file_id":"5536","content_type":"application/pdf","file_name":"IST-2013-113-v1+1_Initiatives_and_projects_related_to_RD.pdf","date_created":"2018-12-12T11:54:14Z"}],"abstract":[{"lang":"eng","text":"This document is created as a part of the project “Repository for Research Data at IST Austria”. It summarises the actual initiatives, projects and standards related to the project. It supports the preparation of standards and specifications for the project, which should be considered and followed to ensure interoperability and visibility of the uploaded data."}],"_id":"5401","department":[{"_id":"E-Lib"}],"month":"03","date_published":"2013-03-20T00:00:00Z","year":"2013","date_updated":"2020-07-14T23:04:47Z","day":"20","oa":1,"language":[{"iso":"eng"}],"ddc":["020"],"date_created":"2018-12-12T11:39:07Z","author":[{"full_name":"Porsche, Jana","id":"3252EDC2-F248-11E8-B48F-1D18A9856A87","first_name":"Jana","last_name":"Porsche"}],"file_date_updated":"2020-07-14T12:46:45Z","citation":{"ieee":"J. Porsche, Initiatives and projects related to RD. IST Austria, 2013.","apa":"Porsche, J. (2013). Initiatives and projects related to RD. IST Austria.","short":"J. Porsche, Initiatives and Projects Related to RD, IST Austria, 2013.","ista":"Porsche J. 2013. Initiatives and projects related to RD, IST Austria,p.","ama":"Porsche J. Initiatives and Projects Related to RD. IST Austria; 2013.","mla":"Porsche, Jana. Initiatives and Projects Related to RD. IST Austria, 2013.","chicago":"Porsche, Jana. Initiatives and Projects Related to RD. IST Austria, 2013."},"publisher":"IST Austria","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","title":"Initiatives and projects related to RD","type":"report","oa_version":"Published Version","pubrep_id":"113","publication_status":"published"},{"file_date_updated":"2020-07-14T12:46:46Z","author":[{"id":"3252EDC2-F248-11E8-B48F-1D18A9856A87","first_name":"Jana","last_name":"Porsche","full_name":"Porsche, Jana"}],"date_created":"2018-12-12T11:39:09Z","language":[{"iso":"eng"}],"ddc":["020"],"oa":1,"pubrep_id":"135","publication_status":"published","oa_version":"Published Version","type":"report","title":"Technical requirements and features","status":"public","publisher":"IST Austria","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"ama":"Porsche J. Technical Requirements and Features. IST Austria; 2013.","ista":"Porsche J. 2013. Technical requirements and features, IST Austria,p.","chicago":"Porsche, Jana. Technical Requirements and Features. IST Austria, 2013.","mla":"Porsche, Jana. Technical Requirements and Features. IST Austria, 2013.","ieee":"J. Porsche, Technical requirements and features. IST Austria, 2013.","short":"J. Porsche, Technical Requirements and Features, IST Austria, 2013.","apa":"Porsche, J. (2013). Technical requirements and features. IST Austria."},"date_published":"2013-07-13T00:00:00Z","month":"07","department":[{"_id":"E-Lib"}],"_id":"5407","abstract":[{"lang":"eng","text":"This document is created as a part of the project “Repository for Research Data at IST Austria”. It summarises the mandatory features, which need to be fulfilled to provide an institutional repository as a platform and also a service to the scientists at the institute. It also includes optional features, which would be of strong benefit for the scientists and would increase the usage of the repository, and hence the visibility of research at IST Austria."}],"file":[{"creator":"system","checksum":"9e4f9abf79a56f651f0012a34909880f","date_updated":"2020-07-14T12:46:46Z","access_level":"open_access","relation":"main_file","file_size":90311,"file_id":"5463","content_type":"application/pdf","date_created":"2018-12-12T11:53:02Z","file_name":"IST-2013-135-v1+1_Features.pdf"}],"has_accepted_license":"1","day":"13","date_updated":"2020-07-14T23:07:51Z","year":"2013"},{"scopus_import":1,"external_id":{"pmid":["23062335"]},"pmid":1,"project":[{"name":"Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen","grant_number":"SFB-TR3-TP10B","_id":"25BDE9A4-B435-11E9-9278-68D0E5697425"}],"abstract":[{"text":"Spontaneous postsynaptic currents (PSCs) provide key information about the mechanisms of synaptic transmission and the activity modes of neuronal networks. However, detecting spontaneous PSCs in vitro and in vivo has been challenging, because of the small amplitude, the variable kinetics, and the undefined time of generation of these events. Here, we describe a, to our knowledge, new method for detecting spontaneous synaptic events by deconvolution, using a template that approximates the average time course of spontaneous PSCs. A recorded PSC trace is deconvolved from the template, resulting in a series of delta-like functions. The maxima of these delta-like events are reliably detected, revealing the precise onset times of the spontaneous PSCs. Among all detection methods, the deconvolution-based method has a unique temporal resolution, allowing the detection of individual events in high-frequency bursts. Furthermore, the deconvolution-based method has a high amplitude resolution, because deconvolution can substantially increase the signal/noise ratio. When tested against previously published methods using experimental data, the deconvolution-based method was superior for spontaneous PSCs recorded in vivo. Using the high-resolution deconvolution-based detection algorithm, we show that the frequency of spontaneous excitatory postsynaptic currents in dentate gyrus granule cells is 4.5 times higher in vivo than in vitro.","lang":"eng"}],"page":"1429 - 1439","quality_controlled":"1","oa_version":"Submitted Version","publisher":"Biophysical","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471482/"}],"issue":"7","date_created":"2018-12-11T12:00:32Z","oa":1,"day":"03","date_updated":"2021-01-12T07:40:01Z","year":"2012","month":"10","date_published":"2012-10-03T00:00:00Z","_id":"2954","department":[{"_id":"PeJo"},{"_id":"ScienComp"}],"publication_status":"published","status":"public","volume":103,"type":"journal_article","citation":{"short":"A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, P.M. Jonas, Biophysical Journal 103 (2012) 1429–1439.","apa":"Pernia-Andrade, A., Goswami, S., Stickler, Y., Fröbe, U., Schlögl, A., & Jonas, P. M. (2012). A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo. Biophysical Journal. Biophysical. https://doi.org/10.1016/j.bpj.2012.08.039","ieee":"A. Pernia-Andrade, S. Goswami, Y. Stickler, U. Fröbe, A. Schlögl, and P. M. Jonas, “A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo,” Biophysical Journal, vol. 103, no. 7. Biophysical, pp. 1429–1439, 2012.","chicago":"Pernia-Andrade, Alejandro, Sarit Goswami, Yvonne Stickler, Ulrich Fröbe, Alois Schlögl, and Peter M Jonas. “A Deconvolution Based Method with High Sensitivity and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro and in Vivo.” Biophysical Journal. Biophysical, 2012. https://doi.org/10.1016/j.bpj.2012.08.039.","mla":"Pernia-Andrade, Alejandro, et al. “A Deconvolution Based Method with High Sensitivity and Temporal Resolution for Detection of Spontaneous Synaptic Currents in Vitro and in Vivo.” Biophysical Journal, vol. 103, no. 7, Biophysical, 2012, pp. 1429–39, doi:10.1016/j.bpj.2012.08.039.","ista":"Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. 2012. A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo. Biophysical Journal. 103(7), 1429–1439.","ama":"Pernia-Andrade A, Goswami S, Stickler Y, Fröbe U, Schlögl A, Jonas PM. A deconvolution based method with high sensitivity and temporal resolution for detection of spontaneous synaptic currents in vitro and in vivo. Biophysical Journal. 2012;103(7):1429-1439. doi:10.1016/j.bpj.2012.08.039"},"author":[{"full_name":"Pernia-Andrade, Alejandro","last_name":"Pernia-Andrade","id":"36963E98-F248-11E8-B48F-1D18A9856A87","first_name":"Alejandro"},{"full_name":"Goswami, Sarit","first_name":"Sarit","id":"3A578F32-F248-11E8-B48F-1D18A9856A87","last_name":"Goswami"},{"full_name":"Stickler, Yvonne","last_name":"Stickler","first_name":"Yvonne","id":"63B76600-E9CC-11E9-9B5F-82450873F7A1"},{"last_name":"Fröbe","first_name":"Ulrich","full_name":"Fröbe, Ulrich"},{"first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87","last_name":"Schlögl","full_name":"Schlögl, Alois","orcid":"0000-0002-5621-8100"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","first_name":"Peter M","last_name":"Jonas","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804"}],"publist_id":"3774","intvolume":" 103","language":[{"iso":"eng"}],"doi":"10.1016/j.bpj.2012.08.039","acknowledgement":"This work was supported by the Deutsche Forschungsgemeinschaft (TR3/B10) and a European Research Council Advanced grant to P.J.\r\nWe thank H. Hu, S. J. Guzman, and C. Schmidt-Hieber for critically reading the manuscript, I. Koeva and F. Marr for technical support, and E. Kramberger for editorial assistance.\r\n","publication":"Biophysical Journal"},{"date_created":"2018-12-11T12:00:30Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publisher":"American Association for the Advancement of Science","title":"Forces driving epithelial spreading in zebrafish gastrulation","oa_version":"None","quality_controlled":"1","acknowledged_ssus":[{"_id":"SSU"}],"issue":"6104","abstract":[{"text":"Contractile actomyosin rings drive various fundamental morphogenetic processes ranging from cytokinesis to wound healing. Actomyosin rings are generally thought to function by circumferential contraction. Here, we show that the spreading of the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation is driven by a contractile actomyosin ring. In contrast to previous suggestions, we find that this ring functions not only by circumferential contraction but also by a flow-friction mechanism. This generates a pulling force through resistance against retrograde actomyosin flow. EVL spreading proceeds normally in situations where circumferential contraction is unproductive, indicating that the flow-friction mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis through a combination of cable-constriction and flow-friction mechanisms.","lang":"eng"}],"page":"257 - 260","project":[{"_id":"252ABD0A-B435-11E9-9278-68D0E5697425","grant_number":"I 930-B20","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","call_identifier":"FWF"}],"scopus_import":1,"publist_id":"3778","author":[{"full_name":"Behrndt, Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin"},{"full_name":"Salbreux, Guillaume","last_name":"Salbreux","first_name":"Guillaume"},{"first_name":"Pedro","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","last_name":"Campinho","full_name":"Campinho, Pedro","orcid":"0000-0002-8526-5416"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert"},{"first_name":"Felix","last_name":"Oswald","full_name":"Oswald, Felix"},{"full_name":"Roensch, Julia","last_name":"Roensch","id":"4220E59C-F248-11E8-B48F-1D18A9856A87","first_name":"Julia"},{"last_name":"Grill","first_name":"Stephan","full_name":"Grill, Stephan"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"doi":"10.1126/science.1224143","publication":"Science","intvolume":" 338","language":[{"iso":"eng"}],"status":"public","type":"journal_article","volume":338,"publication_status":"published","citation":{"ama":"Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading in zebrafish gastrulation. Science. 2012;338(6104):257-260. doi:10.1126/science.1224143","ista":"Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation. Science. 338(6104), 257–260.","chicago":"Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild, Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” Science. American Association for the Advancement of Science, 2012. https://doi.org/10.1126/science.1224143.","mla":"Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.” Science, vol. 338, no. 6104, American Association for the Advancement of Science, 2012, pp. 257–60, doi:10.1126/science.1224143.","ieee":"M. Behrndt et al., “Forces driving epithelial spreading in zebrafish gastrulation,” Science, vol. 338, no. 6104. American Association for the Advancement of Science, pp. 257–260, 2012.","short":"M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch, S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.","apa":"Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch, J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish gastrulation. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1224143"},"month":"10","date_published":"2012-10-12T00:00:00Z","_id":"2950","department":[{"_id":"CaHe"},{"_id":"Bio"}],"day":"12","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"1403"}]},"year":"2012","date_updated":"2023-02-21T17:02:44Z"},{"file":[{"relation":"main_file","access_level":"open_access","file_size":503345,"creator":"system","checksum":"162eea47d9d840c26b496ba6ae4d1c09","date_updated":"2020-07-14T12:45:57Z","date_created":"2018-12-12T10:08:42Z","file_name":"IST-2012-95-v1+1_sp-beitrag_danowski_kontext_open_access_creative_commons.pdf","content_type":"application/pdf","file_id":"4703"}],"page":"200 - 212","has_accepted_license":"1","abstract":[{"text":"Dieser Artikel soll die sechs verschiedenen Creative Commons Lizenzen erläutern und ihre Bedeutung im Rahmen des wissenschaftlichen Publizierens und des Open Access erklären (CC-BY, CC-BY-SA, CC-BY-NC, CC-BY-ND, CC-BYNC-SA, CC-BY-NC-ND).","lang":"eng"}],"scopus_import":1,"ddc":["020"],"oa":1,"date_created":"2018-12-11T12:00:35Z","issue":"2","main_file_link":[{"open_access":"1","url":" http://hdl.handle.net/10760/17625"}],"popular_science":"1","title":"Kontext Open Access: Creative Commons","publisher":"VÖB","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","department":[{"_id":"E-Lib"}],"_id":"2965","date_published":"2012-09-01T00:00:00Z","month":"09","year":"2012","date_updated":"2021-01-12T07:40:07Z","day":"01","publication":"Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare","intvolume":" 65","language":[{"iso":"ger"}],"publist_id":"3754","file_date_updated":"2020-07-14T12:45:57Z","author":[{"orcid":"0000-0002-6026-4409","full_name":"Danowski, Patrick","last_name":"Danowski","first_name":"Patrick","id":"2EBD1598-F248-11E8-B48F-1D18A9856A87"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"mla":"Danowski, Patrick. “Kontext Open Access: Creative Commons.” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare, vol. 65, no. 2, VÖB, 2012, pp. 200–12.","chicago":"Danowski, Patrick. “Kontext Open Access: Creative Commons.” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare. VÖB, 2012.","ista":"Danowski P. 2012. Kontext Open Access: Creative Commons. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare. 65(2), 200–212.","ama":"Danowski P. Kontext Open Access: Creative Commons. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare. 2012;65(2):200-212.","short":"P. Danowski, Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare 65 (2012) 200–212.","apa":"Danowski, P. (2012). Kontext Open Access: Creative Commons. Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare. VÖB.","ieee":"P. Danowski, “Kontext Open Access: Creative Commons,” Mitteilungen der Vereinigung Österreichischer Bibliothekarinnen & Bibliothekare, vol. 65, no. 2. VÖB, pp. 200–212, 2012."},"volume":65,"type":"journal_article","status":"public","publication_status":"published","pubrep_id":"95"},{"scopus_import":1,"file":[{"creator":"system","checksum":"195238221c4b0b0f4035f6f6c16ea17c","date_updated":"2020-07-14T12:46:35Z","relation":"main_file","access_level":"open_access","file_size":2693701,"date_created":"2018-12-12T10:18:34Z","file_name":"IST-2018-945-v1+1_2012_Schloegl_Review_of.pdf","content_type":"application/pdf","file_id":"5356"}],"abstract":[{"text":"The BCI competition IV stands in the tradition of prior BCI competitions that aim to provide high quality neuroscientific data for open access to the scientific community. As experienced already in prior competitions not only scientists from the narrow field of BCI compete, but scholars with a broad variety of backgrounds and nationalities. They include high specialists as well as students.The goals of all BCI competitions have always been to challenge with respect to novel paradigms and complex data. We report on the following challenges: (1) asynchronous data, (2) synthetic, (3) multi-class continuous data, (4) sessionto-session transfer, (5) directionally modulated MEG, (6) finger movements recorded by ECoG. As after past competitions, our hope is that winning entries may enhance the analysis methods of future BCIs.","lang":"eng"}],"has_accepted_license":"1","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publisher":"Frontiers Research Foundation","title":"Review of the BCI competition IV","oa_version":"Published Version","quality_controlled":"1","oa":1,"ddc":["004"],"date_created":"2018-12-11T11:46:46Z","year":"2012","date_updated":"2021-01-12T08:01:03Z","day":"13","_id":"493","department":[{"_id":"ScienComp"},{"_id":"PeJo"}],"month":"07","date_published":"2012-07-13T00:00:00Z","article_number":"55","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"citation":{"ieee":"M. Tangermann et al., “Review of the BCI competition IV,” Frontiers in Neuroscience, vol. 6. Frontiers Research Foundation, 2012.","apa":"Tangermann, M., Müller, K., Aertsen, A., Birbaumer, N., Braun, C., Brunner, C., … Blankertz, B. (2012). Review of the BCI competition IV. Frontiers in Neuroscience. Frontiers Research Foundation. https://doi.org/10.3389/fnins.2012.00055","short":"M. Tangermann, K. Müller, A. Aertsen, N. Birbaumer, C. Braun, C. Brunner, R. Leeb, C. Mehring, K. Miller, G. Müller Putz, G. Nolte, G. Pfurtscheller, H. Preissl, G. Schalk, A. Schlögl, C. Vidaurre, S. Waldert, B. Blankertz, Frontiers in Neuroscience 6 (2012).","ista":"Tangermann M, Müller K, Aertsen A, Birbaumer N, Braun C, Brunner C, Leeb R, Mehring C, Miller K, Müller Putz G, Nolte G, Pfurtscheller G, Preissl H, Schalk G, Schlögl A, Vidaurre C, Waldert S, Blankertz B. 2012. Review of the BCI competition IV. Frontiers in Neuroscience. 6, 55.","ama":"Tangermann M, Müller K, Aertsen A, et al. Review of the BCI competition IV. Frontiers in Neuroscience. 2012;6. doi:10.3389/fnins.2012.00055","chicago":"Tangermann, Michael, Klaus Müller, Ad Aertsen, Niels Birbaumer, Christoph Braun, Clemens Brunner, Robert Leeb, et al. “Review of the BCI Competition IV.” Frontiers in Neuroscience. Frontiers Research Foundation, 2012. https://doi.org/10.3389/fnins.2012.00055.","mla":"Tangermann, Michael, et al. “Review of the BCI Competition IV.” Frontiers in Neuroscience, vol. 6, 55, Frontiers Research Foundation, 2012, doi:10.3389/fnins.2012.00055."},"status":"public","type":"journal_article","volume":6,"publication_status":"published","pubrep_id":"945","acknowledgement":"The studies were in part or completely supported by the Bundesministerium für Bildung und Forschung (BMBF), Fkz 01IB001A, 01GQ0850, by the German Science Foundation (DFG, contract MU 987/3-2), by the European ICT Programme Projects FP7-224631 and 216886, the World Class University Program through the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology (Grant R31-10008), the US Army Research Office [W911NF-08-1-0216 (Gerwin Schalk) and W911NF-07-1-0415 (Gerwin Schalk)] and the NIH [EB006356 (Gerwin Schalk) and EB000856 (Gerwin Schalk), the WIN-Kolleg of the Heidelberg Academy of Sciences and Humanities, German Federal Ministry of Education and Research grants 01GQ0420, 01GQ0761, 01GQ0762, and 01GQ0830, German Research Foundation grants 550/B5 and C6, and by a scholarship from the German National Academic Foundation. This paper only reflects the authors’ views and funding agencies are not liable for any use that may be made of the information contained herein.\r\n","doi":"10.3389/fnins.2012.00055","publication":"Frontiers in Neuroscience","language":[{"iso":"eng"}],"intvolume":" 6","publist_id":"7327","author":[{"last_name":"Tangermann","first_name":"Michael","full_name":"Tangermann, Michael"},{"full_name":"Müller, Klaus","last_name":"Müller","first_name":"Klaus"},{"first_name":"Ad","last_name":"Aertsen","full_name":"Aertsen, Ad"},{"full_name":"Birbaumer, Niels","first_name":"Niels","last_name":"Birbaumer"},{"last_name":"Braun","first_name":"Christoph","full_name":"Braun, Christoph"},{"full_name":"Brunner, Clemens","first_name":"Clemens","last_name":"Brunner"},{"last_name":"Leeb","first_name":"Robert","full_name":"Leeb, Robert"},{"last_name":"Mehring","first_name":"Carsten","full_name":"Mehring, Carsten"},{"last_name":"Miller","first_name":"Kai","full_name":"Miller, Kai"},{"last_name":"Müller Putz","first_name":"Gernot","full_name":"Müller Putz, Gernot"},{"last_name":"Nolte","first_name":"Guido","full_name":"Nolte, Guido"},{"first_name":"Gert","last_name":"Pfurtscheller","full_name":"Pfurtscheller, Gert"},{"full_name":"Preissl, Hubert","last_name":"Preissl","first_name":"Hubert"},{"full_name":"Schalk, Gerwin","last_name":"Schalk","first_name":"Gerwin"},{"orcid":"0000-0002-5621-8100","full_name":"Schlögl, Alois","last_name":"Schlögl","first_name":"Alois","id":"45BF87EE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Vidaurre","first_name":"Carmen","full_name":"Vidaurre, Carmen"},{"full_name":"Waldert, Stephan","last_name":"Waldert","first_name":"Stephan"},{"full_name":"Blankertz, Benjamin","last_name":"Blankertz","first_name":"Benjamin"}],"file_date_updated":"2020-07-14T12:46:35Z"},{"day":"12","year":"2012","date_updated":"2020-07-14T23:04:49Z","month":"11","date_published":"2012-11-12T00:00:00Z","file":[{"content_type":"application/pdf","file_id":"5472","file_name":"IST-2012-103-v1+1_Actual_state_of_research_data_@_IST_Austria.pdf","date_created":"2018-12-12T11:53:11Z","date_updated":"2020-07-14T12:46:44Z","checksum":"e0a7c041eea1ca4b70ab6f9ec5177f4e","creator":"system","file_size":238544,"access_level":"open_access","relation":"main_file"}],"has_accepted_license":"1","abstract":[{"text":"This document is created as a part of the project “Repository for Research Data on IST Austria”. It summarises the actual state of research data at IST Austria, based on survey results. It supports the choice of appropriate software, which would best fit the requirements of their users, the researchers.","lang":"eng"}],"_id":"5398","department":[{"_id":"E-Lib"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"IST Austria","type":"report","title":"Actual state of research data @ ISTAustria","publication_status":"published","pubrep_id":"103","oa_version":"Published Version","citation":{"apa":"Porsche, J. (2012). Actual state of research data @ ISTAustria. IST Austria.","short":"J. Porsche, Actual State of Research Data @ ISTAustria, IST Austria, 2012.","ieee":"J. Porsche, Actual state of research data @ ISTAustria. IST Austria, 2012.","chicago":"Porsche, Jana. Actual State of Research Data @ ISTAustria. IST Austria, 2012.","mla":"Porsche, Jana. Actual State of Research Data @ ISTAustria. IST Austria, 2012.","ama":"Porsche J. Actual State of Research Data @ ISTAustria. IST Austria; 2012.","ista":"Porsche J. 2012. Actual state of research data @ ISTAustria, IST Austria,p."},"date_created":"2018-12-12T11:39:06Z","author":[{"first_name":"Jana","id":"3252EDC2-F248-11E8-B48F-1D18A9856A87","last_name":"Porsche","full_name":"Porsche, Jana"}],"file_date_updated":"2020-07-14T12:46:44Z","oa":1,"ddc":["020"],"language":[{"iso":"eng"}]}]