---
_id: '453'
abstract:
- lang: eng
text: Most kinesin motors move in only one direction along microtubules. Members
of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed
motors and shown to produce piconewton forces. However, some fungal kinesin-5
motors are bidirectional. The force production of a bidirectional kinesin-5 has
not yet been measured. Therefore, it remains unknown whether the mechanism of
the unconventional minus-end-directed motility differs fundamentally from that
of plus-end-directed stepping. Using force spectroscopy, we have measured here
the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in
microtubule gliding assays in both plus- and minus-end direction. Correlation
analysis of pause forces demonstrated that individual Cin8 molecules produce additive
forces in both directions of movement. In ensembles, Cin8 motors were able to
produce single-motor forces up to a magnitude of ∼1.5 pN. Hence, these properties
appear to be conserved within the kinesin-5 subfamily. Force production was largely
independent of the directionality of movement, indicating similarities between
the motility mechanisms for both directions. These results provide constraints
for the development of models for the bidirectional motility mechanism of fission
yeast kinesin-5 and provide insight into the function of this mitotic motor.
acknowledgement: 'The plasmid for full-length kinesin-1 was a gift from G. Holzwarth
and J. Macosko with permission from J. Howard. We thank I. Lueke and N. I. Cade
for technical assistance. G.P. thanks the Francis Crick Institute, and in particular
the Surrey and Salbreux groups, for their hospitality during his sabbatical stay,
as well as Imperial College London for making it possible. This work was supported
by the Francis Crick Institute, which receives its core funding from Cancer Research
UK (FC001163), the United Kingdom Medical Research Council (FC001163), and the Wellcome
Trust (FC001163), and by Imperial College London. J.R. was also supported by a Sir
Henry Wellcome Postdoctoral Fellowship (100145/Z/12/Z) and T.S. by the European
Research Council (Advanced Grant, project 323042). '
article_processing_charge: No
article_type: original
author:
- first_name: Todd
full_name: Fallesen, Todd
last_name: Fallesen
- first_name: Johanna
full_name: Roostalu, Johanna
last_name: Roostalu
- first_name: Christian F
full_name: Düllberg, Christian F
id: 459064DC-F248-11E8-B48F-1D18A9856A87
last_name: Düllberg
orcid: 0000-0001-6335-9748
- first_name: Gunnar
full_name: Pruessner, Gunnar
last_name: Pruessner
- first_name: Thomas
full_name: Surrey, Thomas
last_name: Surrey
citation:
ama: Fallesen T, Roostalu J, Düllberg CF, Pruessner G, Surrey T. Ensembles of bidirectional
kinesin Cin8 produce additive forces in both directions of movement. Biophysical
Journal. 2017;113(9):2055-2067. doi:10.1016/j.bpj.2017.09.006
apa: Fallesen, T., Roostalu, J., Düllberg, C. F., Pruessner, G., & Surrey, T.
(2017). Ensembles of bidirectional kinesin Cin8 produce additive forces in both
directions of movement. Biophysical Journal. Biophysical Society. https://doi.org/10.1016/j.bpj.2017.09.006
chicago: Fallesen, Todd, Johanna Roostalu, Christian F Düllberg, Gunnar Pruessner,
and Thomas Surrey. “Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces
in Both Directions of Movement.” Biophysical Journal. Biophysical Society,
2017. https://doi.org/10.1016/j.bpj.2017.09.006.
ieee: T. Fallesen, J. Roostalu, C. F. Düllberg, G. Pruessner, and T. Surrey, “Ensembles
of bidirectional kinesin Cin8 produce additive forces in both directions of movement,”
Biophysical Journal, vol. 113, no. 9. Biophysical Society, pp. 2055–2067,
2017.
ista: Fallesen T, Roostalu J, Düllberg CF, Pruessner G, Surrey T. 2017. Ensembles
of bidirectional kinesin Cin8 produce additive forces in both directions of movement.
Biophysical Journal. 113(9), 2055–2067.
mla: Fallesen, Todd, et al. “Ensembles of Bidirectional Kinesin Cin8 Produce Additive
Forces in Both Directions of Movement.” Biophysical Journal, vol. 113,
no. 9, Biophysical Society, 2017, pp. 2055–67, doi:10.1016/j.bpj.2017.09.006.
short: T. Fallesen, J. Roostalu, C.F. Düllberg, G. Pruessner, T. Surrey, Biophysical
Journal 113 (2017) 2055–2067.
date_created: 2018-12-11T11:46:33Z
date_published: 2017-11-07T00:00:00Z
date_updated: 2021-01-12T07:59:28Z
day: '07'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1016/j.bpj.2017.09.006
file:
- access_level: open_access
checksum: 99a2474088e20ac74b1882c4fbbb45b1
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:03Z
date_updated: 2020-07-14T12:46:31Z
file_id: '5052'
file_name: IST-2018-965-v1+1_2017_Duellberg_Ensembles_of.pdf
file_size: 977192
relation: main_file
file_date_updated: 2020-07-14T12:46:31Z
has_accepted_license: '1'
intvolume: ' 113'
issue: '9'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
page: 2055 - 2067
publication: Biophysical Journal
publication_status: published
publisher: Biophysical Society
publist_id: '7369'
pubrep_id: '965'
quality_controlled: '1'
status: public
title: Ensembles of bidirectional kinesin Cin8 produce additive forces in both directions
of movement
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2017'
...
---
_id: '464'
abstract:
- lang: eng
text: The computation of the winning set for parity objectives and for Streett objectives
in graphs as well as in game graphs are central problems in computer-aided verification,
with application to the verification of closed systems with strong fairness conditions,
the verification of open systems, checking interface compatibility, well-formedness
of specifications, and the synthesis of reactive systems. We show how to compute
the winning set on n vertices for (1) parity-3 (aka one-pair Streett) objectives
in game graphs in time O(n5/2) and for (2) k-pair Streett objectives in graphs
in time O(n2+nklogn). For both problems this gives faster algorithms for dense
graphs and represents the first improvement in asymptotic running time in 15 years.
article_number: '26'
article_processing_charge: No
author:
- first_name: Krishnendu
full_name: Chatterjee, Krishnendu
id: 2E5DCA20-F248-11E8-B48F-1D18A9856A87
last_name: Chatterjee
orcid: 0000-0002-4561-241X
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
- first_name: Veronika
full_name: Loitzenbauer, Veronika
last_name: Loitzenbauer
citation:
ama: Chatterjee K, Henzinger MH, Loitzenbauer V. Improved algorithms for parity
and Streett objectives. Logical Methods in Computer Science. 2017;13(3).
doi:10.23638/LMCS-13(3:26)2017
apa: Chatterjee, K., Henzinger, M. H., & Loitzenbauer, V. (2017). Improved algorithms
for parity and Streett objectives. Logical Methods in Computer Science.
International Federation of Computational Logic. https://doi.org/10.23638/LMCS-13(3:26)2017
chicago: Chatterjee, Krishnendu, Monika H Henzinger, and Veronika Loitzenbauer.
“Improved Algorithms for Parity and Streett Objectives.” Logical Methods in
Computer Science. International Federation of Computational Logic, 2017. https://doi.org/10.23638/LMCS-13(3:26)2017.
ieee: K. Chatterjee, M. H. Henzinger, and V. Loitzenbauer, “Improved algorithms
for parity and Streett objectives,” Logical Methods in Computer Science,
vol. 13, no. 3. International Federation of Computational Logic, 2017.
ista: Chatterjee K, Henzinger MH, Loitzenbauer V. 2017. Improved algorithms for
parity and Streett objectives. Logical Methods in Computer Science. 13(3), 26.
mla: Chatterjee, Krishnendu, et al. “Improved Algorithms for Parity and Streett
Objectives.” Logical Methods in Computer Science, vol. 13, no. 3, 26, International
Federation of Computational Logic, 2017, doi:10.23638/LMCS-13(3:26)2017.
short: K. Chatterjee, M.H. Henzinger, V. Loitzenbauer, Logical Methods in Computer
Science 13 (2017).
date_created: 2018-12-11T11:46:37Z
date_published: 2017-09-26T00:00:00Z
date_updated: 2023-02-23T10:08:55Z
day: '26'
ddc:
- '004'
department:
- _id: KrCh
doi: 10.23638/LMCS-13(3:26)2017
ec_funded: 1
external_id:
arxiv:
- '1410.0833'
file:
- access_level: open_access
checksum: 12d469ae69b80361333d7dead965cf5d
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:27Z
date_updated: 2020-07-14T12:46:32Z
file_id: '5010'
file_name: IST-2018-956-v1+1_2017_Chatterjee_Improved_algorithms.pdf
file_size: 582940
relation: main_file
file_date_updated: 2020-07-14T12:46:32Z
has_accepted_license: '1'
intvolume: ' 13'
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 2584A770-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P 23499-N23
name: Modern Graph Algorithmic Techniques in Formal Verification
- _id: 25863FF4-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: S11407
name: Game Theory
- _id: 25892FC0-B435-11E9-9278-68D0E5697425
grant_number: ICT15-003
name: Efficient Algorithms for Computer Aided Verification
- _id: 2581B60A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '279307'
name: 'Quantitative Graph Games: Theory and Applications'
publication: Logical Methods in Computer Science
publication_identifier:
issn:
- 1860-5974
publication_status: published
publisher: International Federation of Computational Logic
publist_id: '7357'
pubrep_id: '956'
quality_controlled: '1'
related_material:
record:
- id: '1661'
relation: earlier_version
status: public
scopus_import: '1'
status: public
title: Improved algorithms for parity and Streett objectives
tmp:
image: /image/cc_by_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nd/4.0/legalcode
name: Creative Commons Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0)
short: CC BY-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2017'
...
---
_id: '470'
abstract:
- lang: eng
text: This paper presents a method for simulating water surface waves as a displacement
field on a 2D domain. Our method relies on Lagrangian particles that carry packets
of water wave energy; each packet carries information about an entire group of
wave trains, as opposed to only a single wave crest. Our approach is unconditionally
stable and can simulate high resolution geometric details. This approach also
presents a straightforward interface for artistic control, because it is essentially
a particle system with intuitive parameters like wavelength and amplitude. Our
implementation parallelizes well and runs in real time for moderately challenging
scenarios.
acknowledged_ssus:
- _id: ScienComp
article_number: '103'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Stefan
full_name: Jeschke, Stefan
id: 44D6411A-F248-11E8-B48F-1D18A9856A87
last_name: Jeschke
- first_name: Christopher J
full_name: Wojtan, Christopher J
id: 3C61F1D2-F248-11E8-B48F-1D18A9856A87
last_name: Wojtan
orcid: 0000-0001-6646-5546
citation:
ama: Jeschke S, Wojtan C. Water wave packets. ACM Transactions on Graphics.
2017;36(4). doi:10.1145/3072959.3073678
apa: Jeschke, S., & Wojtan, C. (2017). Water wave packets. ACM Transactions
on Graphics. ACM. https://doi.org/10.1145/3072959.3073678
chicago: Jeschke, Stefan, and Chris Wojtan. “Water Wave Packets.” ACM Transactions
on Graphics. ACM, 2017. https://doi.org/10.1145/3072959.3073678.
ieee: S. Jeschke and C. Wojtan, “Water wave packets,” ACM Transactions on Graphics,
vol. 36, no. 4. ACM, 2017.
ista: Jeschke S, Wojtan C. 2017. Water wave packets. ACM Transactions on Graphics.
36(4), 103.
mla: Jeschke, Stefan, and Chris Wojtan. “Water Wave Packets.” ACM Transactions
on Graphics, vol. 36, no. 4, 103, ACM, 2017, doi:10.1145/3072959.3073678.
short: S. Jeschke, C. Wojtan, ACM Transactions on Graphics 36 (2017).
date_created: 2018-12-11T11:46:39Z
date_published: 2017-07-01T00:00:00Z
date_updated: 2023-02-23T12:20:26Z
day: '01'
ddc:
- '006'
department:
- _id: ChWo
doi: 10.1145/3072959.3073678
ec_funded: 1
file:
- access_level: open_access
checksum: 82a3b2bfeee4ddef16ecc21675d1a48a
content_type: application/pdf
creator: wojtan
date_created: 2020-01-24T09:32:35Z
date_updated: 2020-07-14T12:46:34Z
file_id: '7359'
file_name: wavepackets_final.pdf
file_size: 13131683
relation: main_file
file_date_updated: 2020-07-14T12:46:34Z
has_accepted_license: '1'
intvolume: ' 36'
issue: '4'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 2533E772-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '638176'
name: Efficient Simulation of Natural Phenomena at Extremely Large Scales
publication: ACM Transactions on Graphics
publication_identifier:
issn:
- '07300301'
publication_status: published
publisher: ACM
publist_id: '7350'
quality_controlled: '1'
scopus_import: 1
status: public
title: Water wave packets
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 36
year: '2017'
...
---
_id: '471'
abstract:
- lang: eng
text: 'We present a new algorithm for the statistical model checking of Markov chains
with respect to unbounded temporal properties, including full linear temporal
logic. The main idea is that we monitor each simulation run on the fly, in order
to detect quickly if a bottom strongly connected component is entered with high
probability, in which case the simulation run can be terminated early. As a result,
our simulation runs are often much shorter than required by termination bounds
that are computed a priori for a desired level of confidence on a large state
space. In comparison to previous algorithms for statistical model checking our
method is not only faster in many cases but also requires less information about
the system, namely, only the minimum transition probability that occurs in the
Markov chain. In addition, our method can be generalised to unbounded quantitative
properties such as mean-payoff bounds. '
article_number: '12'
author:
- first_name: Przemyslaw
full_name: Daca, Przemyslaw
id: 49351290-F248-11E8-B48F-1D18A9856A87
last_name: Daca
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000−0002−2985−7724
- first_name: Jan
full_name: Kretinsky, Jan
id: 44CEF464-F248-11E8-B48F-1D18A9856A87
last_name: Kretinsky
orcid: 0000-0002-8122-2881
- first_name: Tatjana
full_name: Petrov, Tatjana
id: 3D5811FC-F248-11E8-B48F-1D18A9856A87
last_name: Petrov
orcid: 0000-0002-9041-0905
citation:
ama: Daca P, Henzinger TA, Kretinsky J, Petrov T. Faster statistical model checking
for unbounded temporal properties. ACM Transactions on Computational Logic
(TOCL). 2017;18(2). doi:10.1145/3060139
apa: Daca, P., Henzinger, T. A., Kretinsky, J., & Petrov, T. (2017). Faster
statistical model checking for unbounded temporal properties. ACM Transactions
on Computational Logic (TOCL). ACM. https://doi.org/10.1145/3060139
chicago: Daca, Przemyslaw, Thomas A Henzinger, Jan Kretinsky, and Tatjana Petrov.
“Faster Statistical Model Checking for Unbounded Temporal Properties.” ACM
Transactions on Computational Logic (TOCL). ACM, 2017. https://doi.org/10.1145/3060139.
ieee: P. Daca, T. A. Henzinger, J. Kretinsky, and T. Petrov, “Faster statistical
model checking for unbounded temporal properties,” ACM Transactions on Computational
Logic (TOCL), vol. 18, no. 2. ACM, 2017.
ista: Daca P, Henzinger TA, Kretinsky J, Petrov T. 2017. Faster statistical model
checking for unbounded temporal properties. ACM Transactions on Computational
Logic (TOCL). 18(2), 12.
mla: Daca, Przemyslaw, et al. “Faster Statistical Model Checking for Unbounded Temporal
Properties.” ACM Transactions on Computational Logic (TOCL), vol. 18, no.
2, 12, ACM, 2017, doi:10.1145/3060139.
short: P. Daca, T.A. Henzinger, J. Kretinsky, T. Petrov, ACM Transactions on Computational
Logic (TOCL) 18 (2017).
date_created: 2018-12-11T11:46:39Z
date_published: 2017-05-01T00:00:00Z
date_updated: 2023-02-21T16:48:11Z
day: '01'
department:
- _id: ToHe
doi: 10.1145/3060139
ec_funded: 1
intvolume: ' 18'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1504.05739
month: '05'
oa: 1
oa_version: Submitted Version
project:
- _id: 25EE3708-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '267989'
name: Quantitative Reactive Modeling
- _id: 25F5A88A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: S11402-N23
name: Moderne Concurrency Paradigms
- _id: 25F42A32-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: Z211
name: The Wittgenstein Prize
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: ACM Transactions on Computational Logic (TOCL)
publication_identifier:
issn:
- '15293785'
publication_status: published
publisher: ACM
publist_id: '7349'
quality_controlled: '1'
related_material:
record:
- id: '1234'
relation: earlier_version
status: public
scopus_import: 1
status: public
title: Faster statistical model checking for unbounded temporal properties
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2017'
...
---
_id: '481'
abstract:
- lang: eng
text: We introduce planar matchings on directed pseudo-line arrangements, which
yield a planar set of pseudo-line segments such that only matching-partners are
adjacent. By translating the planar matching problem into a corresponding stable
roommates problem we show that such matchings always exist. Using our new framework,
we establish, for the first time, a complete, rigorous definition of weighted
straight skeletons, which are based on a so-called wavefront propagation process.
We present a generalized and unified approach to treat structural changes in the
wavefront that focuses on the restoration of weak planarity by finding planar
matchings.
acknowledgement: 'Supported by NSERC and the Ross and Muriel Cheriton Fellowship.
Research supported by Austrian Science Fund (FWF): P25816-N15.'
author:
- first_name: Therese
full_name: Biedl, Therese
last_name: Biedl
- first_name: Stefan
full_name: Huber, Stefan
id: 4700A070-F248-11E8-B48F-1D18A9856A87
last_name: Huber
orcid: 0000-0002-8871-5814
- first_name: Peter
full_name: Palfrader, Peter
last_name: Palfrader
citation:
ama: Biedl T, Huber S, Palfrader P. Planar matchings for weighted straight skeletons.
International Journal of Computational Geometry and Applications. 2017;26(3-4):211-229.
doi:10.1142/S0218195916600050
apa: Biedl, T., Huber, S., & Palfrader, P. (2017). Planar matchings for weighted
straight skeletons. International Journal of Computational Geometry and Applications.
World Scientific Publishing. https://doi.org/10.1142/S0218195916600050
chicago: Biedl, Therese, Stefan Huber, and Peter Palfrader. “Planar Matchings for
Weighted Straight Skeletons.” International Journal of Computational Geometry
and Applications. World Scientific Publishing, 2017. https://doi.org/10.1142/S0218195916600050.
ieee: T. Biedl, S. Huber, and P. Palfrader, “Planar matchings for weighted straight
skeletons,” International Journal of Computational Geometry and Applications,
vol. 26, no. 3–4. World Scientific Publishing, pp. 211–229, 2017.
ista: Biedl T, Huber S, Palfrader P. 2017. Planar matchings for weighted straight
skeletons. International Journal of Computational Geometry and Applications. 26(3–4),
211–229.
mla: Biedl, Therese, et al. “Planar Matchings for Weighted Straight Skeletons.”
International Journal of Computational Geometry and Applications, vol.
26, no. 3–4, World Scientific Publishing, 2017, pp. 211–29, doi:10.1142/S0218195916600050.
short: T. Biedl, S. Huber, P. Palfrader, International Journal of Computational
Geometry and Applications 26 (2017) 211–229.
date_created: 2018-12-11T11:46:43Z
date_published: 2017-04-13T00:00:00Z
date_updated: 2023-02-21T16:06:22Z
day: '13'
ddc:
- '004'
- '514'
- '516'
department:
- _id: HeEd
doi: 10.1142/S0218195916600050
file:
- access_level: open_access
checksum: f79e8558bfe4b368dfefeb8eec2e3a5e
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:09:34Z
date_updated: 2020-07-14T12:46:35Z
file_id: '4758'
file_name: IST-2018-949-v1+1_2016_huber_PLanar_matchings.pdf
file_size: 769296
relation: main_file
file_date_updated: 2020-07-14T12:46:35Z
has_accepted_license: '1'
intvolume: ' 26'
issue: 3-4
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 211 - 229
publication: International Journal of Computational Geometry and Applications
publication_status: published
publisher: World Scientific Publishing
publist_id: '7338'
pubrep_id: '949'
quality_controlled: '1'
related_material:
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relation: earlier_version
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scopus_import: 1
status: public
title: Planar matchings for weighted straight skeletons
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
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user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
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year: '2017'
...