---
_id: '2031'
abstract:
- lang: eng
text: A puzzling property of synaptic transmission, originally established at the
neuromuscular junction, is that the time course of transmitter release is independent
of the extracellular Ca2+ concentration ([Ca2+]o), whereas the rate of release
is highly [Ca2+]o-dependent. Here, we examine the time course of release at inhibitory
basket cell-Purkinje cell synapses and show that it is independent of [Ca2+]o.
Modeling of Ca2+-dependent transmitter release suggests that the invariant time
course of release critically depends on tight coupling between Ca2+ channels and
release sensors. Experiments with exogenous Ca2+ chelators reveal that channel-sensor
coupling at basket cell-Purkinje cell synapses is very tight, with a mean distance
of 10–20 nm. Thus, tight channel-sensor coupling provides a mechanistic explanation
for the apparent [Ca2+]o independence of the time course of release.
author:
- first_name: Itaru
full_name: Arai, Itaru
id: 32A73F6C-F248-11E8-B48F-1D18A9856A87
last_name: Arai
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Arai itaru, Jonas PM. Nanodomain coupling explains Ca^2+ independence of transmitter
release time course at a fast central synapse. eLife. 2014;3. doi:10.7554/eLife.04057
apa: Arai, itaru, & Jonas, P. M. (2014). Nanodomain coupling explains Ca^2+
independence of transmitter release time course at a fast central synapse. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.04057
chicago: Arai, itaru, and Peter M Jonas. “Nanodomain Coupling Explains Ca^2+ Independence
of Transmitter Release Time Course at a Fast Central Synapse.” ELife. eLife
Sciences Publications, 2014. https://doi.org/10.7554/eLife.04057.
ieee: itaru Arai and P. M. Jonas, “Nanodomain coupling explains Ca^2+ independence
of transmitter release time course at a fast central synapse,” eLife, vol.
3. eLife Sciences Publications, 2014.
ista: Arai itaru, Jonas PM. 2014. Nanodomain coupling explains Ca^2+ independence
of transmitter release time course at a fast central synapse. eLife. 3.
mla: Arai, itaru, and Peter M. Jonas. “Nanodomain Coupling Explains Ca^2+ Independence
of Transmitter Release Time Course at a Fast Central Synapse.” ELife, vol.
3, eLife Sciences Publications, 2014, doi:10.7554/eLife.04057.
short: itaru Arai, P.M. Jonas, ELife 3 (2014).
date_created: 2018-12-11T11:55:19Z
date_published: 2014-12-09T00:00:00Z
date_updated: 2021-01-12T06:54:51Z
day: '09'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.7554/eLife.04057
ec_funded: 1
file:
- access_level: open_access
checksum: c240f915450d4ebe8f95043a2a8c7b1a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:41Z
date_updated: 2020-07-14T12:45:26Z
file_id: '5094'
file_name: IST-2016-421-v1+1_e04057.full.pdf
file_size: 2239563
relation: main_file
file_date_updated: 2020-07-14T12:45:26Z
has_accepted_license: '1'
intvolume: ' 3'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Submitted Version
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5041'
pubrep_id: '421'
quality_controlled: '1'
scopus_import: 1
status: public
title: Nanodomain coupling explains Ca^2+ independence of transmitter release time
course at a fast central synapse
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2014'
...
---
_id: '2041'
abstract:
- lang: eng
text: The hippocampus mediates several higher brain functions, such as learning,
memory, and spatial coding. The input region of the hippocampus, the dentate gyrus,
plays a critical role in these processes. Several lines of evidence suggest that
the dentate gyrus acts as a preprocessor of incoming information, preparing it
for subsequent processing in CA3. For example, the dentate gyrus converts input
from the entorhinal cortex, where cells have multiple spatial fields, into the
spatially more specific place cell activity characteristic of the CA3 region.
Furthermore, the dentate gyrus is involved in pattern separation, transforming
relatively similar input patterns into substantially different output patterns.
Finally, the dentate gyrus produces a very sparse coding scheme in which only
a very small fraction of neurons are active at any one time.
article_number: 2p
author:
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: John
full_name: Lisman, John
last_name: Lisman
citation:
ama: Jonas PM, Lisman J. Structure, function and plasticity of hippocampal dentate
gyrus microcircuits. Frontiers in Neural Circuits. 2014;8. doi:10.3389/fncir.2014.00107
apa: Jonas, P. M., & Lisman, J. (2014). Structure, function and plasticity of
hippocampal dentate gyrus microcircuits. Frontiers in Neural Circuits.
Frontiers Research Foundation. https://doi.org/10.3389/fncir.2014.00107
chicago: Jonas, Peter M, and John Lisman. “Structure, Function and Plasticity of
Hippocampal Dentate Gyrus Microcircuits.” Frontiers in Neural Circuits.
Frontiers Research Foundation, 2014. https://doi.org/10.3389/fncir.2014.00107.
ieee: P. M. Jonas and J. Lisman, “Structure, function and plasticity of hippocampal
dentate gyrus microcircuits,” Frontiers in Neural Circuits, vol. 8. Frontiers
Research Foundation, 2014.
ista: Jonas PM, Lisman J. 2014. Structure, function and plasticity of hippocampal
dentate gyrus microcircuits. Frontiers in Neural Circuits. 8, 2p.
mla: Jonas, Peter M., and John Lisman. “Structure, Function and Plasticity of Hippocampal
Dentate Gyrus Microcircuits.” Frontiers in Neural Circuits, vol. 8, 2p,
Frontiers Research Foundation, 2014, doi:10.3389/fncir.2014.00107.
short: P.M. Jonas, J. Lisman, Frontiers in Neural Circuits 8 (2014).
date_created: 2018-12-11T11:55:22Z
date_published: 2014-09-10T00:00:00Z
date_updated: 2021-01-12T06:54:55Z
day: '10'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.3389/fncir.2014.00107
file:
- access_level: open_access
checksum: 3ca57b164045523f876407e9f13a9fb8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:38Z
date_updated: 2020-07-14T12:45:26Z
file_id: '5294'
file_name: IST-2016-424-v1+1_fncir-08-00107.pdf
file_size: 201110
relation: main_file
file_date_updated: 2020-07-14T12:45:26Z
has_accepted_license: '1'
intvolume: ' 8'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
publication: Frontiers in Neural Circuits
publication_status: published
publisher: Frontiers Research Foundation
publist_id: '5010'
pubrep_id: '424'
quality_controlled: '1'
scopus_import: 1
status: public
title: Structure, function and plasticity of hippocampal dentate gyrus microcircuits
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2014'
...
---
_id: '2062'
abstract:
- lang: eng
text: The success story of fast-spiking, parvalbumin-positive (PV+) GABAergic interneurons
(GABA, γ-aminobutyric acid) in the mammalian central nervous system is noteworthy.
In 1995, the properties of these interneurons were completely unknown. Twenty
years later, thanks to the massive use of subcellular patch-clamp techniques,
simultaneous multiple-cell recording, optogenetics, in vivo measurements, and
computational approaches, our knowledge about PV+ interneurons became more extensive
than for several types of pyramidal neurons. These findings have implications
beyond the “small world” of basic research on GABAergic cells. For example, the
results provide a first proof of principle that neuroscientists might be able
to close the gaps between the molecular, cellular, network, and behavioral levels,
representing one of the main challenges at the present time. Furthermore, the
results may form the basis for PV+ interneurons as therapeutic targets for brain
disease in the future. However, much needs to be learned about the basic function
of these interneurons before clinical neuroscientists will be able to use PV+
interneurons for therapeutic purposes.
article_number: '1255263'
author:
- first_name: Hua
full_name: Hu, Hua
id: 4AC0145C-F248-11E8-B48F-1D18A9856A87
last_name: Hu
- first_name: Jian
full_name: Gan, Jian
id: 3614E438-F248-11E8-B48F-1D18A9856A87
last_name: Gan
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: 'Hu H, Gan J, Jonas PM. Fast-spiking parvalbumin^+ GABAergic interneurons:
From cellular design to microcircuit function. Science. 2014;345(6196).
doi:10.1126/science.1255263'
apa: 'Hu, H., Gan, J., & Jonas, P. M. (2014). Fast-spiking parvalbumin^+ GABAergic
interneurons: From cellular design to microcircuit function. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.1255263'
chicago: 'Hu, Hua, Jian Gan, and Peter M Jonas. “Fast-Spiking Parvalbumin^+ GABAergic
Interneurons: From Cellular Design to Microcircuit Function.” Science.
American Association for the Advancement of Science, 2014. https://doi.org/10.1126/science.1255263.'
ieee: 'H. Hu, J. Gan, and P. M. Jonas, “Fast-spiking parvalbumin^+ GABAergic interneurons:
From cellular design to microcircuit function,” Science, vol. 345, no.
6196. American Association for the Advancement of Science, 2014.'
ista: 'Hu H, Gan J, Jonas PM. 2014. Fast-spiking parvalbumin^+ GABAergic interneurons:
From cellular design to microcircuit function. Science. 345(6196), 1255263.'
mla: 'Hu, Hua, et al. “Fast-Spiking Parvalbumin^+ GABAergic Interneurons: From Cellular
Design to Microcircuit Function.” Science, vol. 345, no. 6196, 1255263,
American Association for the Advancement of Science, 2014, doi:10.1126/science.1255263.'
short: H. Hu, J. Gan, P.M. Jonas, Science 345 (2014).
date_created: 2018-12-11T11:55:29Z
date_published: 2014-08-01T00:00:00Z
date_updated: 2021-01-12T06:55:03Z
day: '01'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.1126/science.1255263
ec_funded: 1
file:
- access_level: open_access
checksum: a0036a589037d37e86364fa25cc0a82f
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:00Z
date_updated: 2020-07-14T12:45:27Z
file_id: '5185'
file_name: IST-2017-821-v1+1_1255263JonasPVReviewTextR_Final.pdf
file_size: 215514
relation: main_file
- access_level: open_access
checksum: e1f57d2713725449cb898fdcb8ef47b8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:01Z
date_updated: 2020-07-14T12:45:27Z
file_id: '5186'
file_name: IST-2017-821-v1+2_1255263JonasPVReviewFigures_Final.pdf
file_size: 1732723
relation: main_file
file_date_updated: 2020-07-14T12:45:27Z
has_accepted_license: '1'
intvolume: ' 345'
issue: '6196'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Submitted Version
project:
- _id: 25C26B1E-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P24909-B24
name: Mechanisms of transmitter release at GABAergic synapses
- _id: 25C0F108-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '268548'
name: Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '4984'
pubrep_id: '821'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Fast-spiking parvalbumin^+ GABAergic interneurons: From cellular design to
microcircuit function'
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 345
year: '2014'
...
---
_id: '2164'
abstract:
- lang: eng
text: 'Neuronal ectopia, such as granule cell dispersion (GCD) in temporal lobe
epilepsy (TLE), has been assumed to result from a migration defect during development.
Indeed, recent studies reported that aberrant migration of neonatal-generated
dentate granule cells (GCs) increased the risk to develop epilepsy later in life.
On the contrary, in the present study, we show that fully differentiated GCs become
motile following the induction of epileptiform activity, resulting in GCD. Hippocampal
slice cultures from transgenic mice expressing green fluorescent protein in differentiated,
but not in newly generated GCs, were incubated with the glutamate receptor agonist
kainate (KA), which induced GC burst activity and GCD. Using real-time microscopy,
we observed that KA-exposed, differentiated GCs translocated their cell bodies
and changed their dendritic organization. As found in human TLE, KA application
was associated with decreased expression of the extracellular matrix protein Reelin,
particularly in hilar interneurons. Together these findings suggest that KA-induced
motility of differentiated GCs contributes to the development of GCD and establish
slice cultures as a model to study neuronal changes induced by epileptiform activity. '
author:
- first_name: Xuejun
full_name: Chai, Xuejun
last_name: Chai
- first_name: Gert
full_name: Münzner, Gert
last_name: Münzner
- first_name: Shanting
full_name: Zhao, Shanting
last_name: Zhao
- first_name: Stefanie
full_name: Tinnes, Stefanie
last_name: Tinnes
- first_name: Janina
full_name: Kowalski, Janina
id: 3F3CA136-F248-11E8-B48F-1D18A9856A87
last_name: Kowalski
- first_name: Ute
full_name: Häussler, Ute
last_name: Häussler
- first_name: Christina
full_name: Young, Christina
last_name: Young
- first_name: Carola
full_name: Haas, Carola
last_name: Haas
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
citation:
ama: Chai X, Münzner G, Zhao S, et al. Epilepsy-induced motility of differentiated
neurons. Cerebral Cortex. 2014;24(8):2130-2140. doi:10.1093/cercor/bht067
apa: Chai, X., Münzner, G., Zhao, S., Tinnes, S., Kowalski, J., Häussler, U., …
Frotscher, M. (2014). Epilepsy-induced motility of differentiated neurons. Cerebral
Cortex. Oxford University Press. https://doi.org/10.1093/cercor/bht067
chicago: Chai, Xuejun, Gert Münzner, Shanting Zhao, Stefanie Tinnes, Janina Kowalski,
Ute Häussler, Christina Young, Carola Haas, and Michael Frotscher. “Epilepsy-Induced
Motility of Differentiated Neurons.” Cerebral Cortex. Oxford University
Press, 2014. https://doi.org/10.1093/cercor/bht067.
ieee: X. Chai et al., “Epilepsy-induced motility of differentiated neurons,”
Cerebral Cortex, vol. 24, no. 8. Oxford University Press, pp. 2130–2140,
2014.
ista: Chai X, Münzner G, Zhao S, Tinnes S, Kowalski J, Häussler U, Young C, Haas
C, Frotscher M. 2014. Epilepsy-induced motility of differentiated neurons. Cerebral
Cortex. 24(8), 2130–2140.
mla: Chai, Xuejun, et al. “Epilepsy-Induced Motility of Differentiated Neurons.”
Cerebral Cortex, vol. 24, no. 8, Oxford University Press, 2014, pp. 2130–40,
doi:10.1093/cercor/bht067.
short: X. Chai, G. Münzner, S. Zhao, S. Tinnes, J. Kowalski, U. Häussler, C. Young,
C. Haas, M. Frotscher, Cerebral Cortex 24 (2014) 2130–2140.
date_created: 2018-12-11T11:56:04Z
date_published: 2014-08-01T00:00:00Z
date_updated: 2021-01-12T06:55:43Z
day: '01'
department:
- _id: PeJo
doi: 10.1093/cercor/bht067
intvolume: ' 24'
issue: '8'
language:
- iso: eng
month: '08'
oa_version: None
page: 2130 - 2140
publication: Cerebral Cortex
publication_status: published
publisher: Oxford University Press
publist_id: '4820'
quality_controlled: '1'
scopus_import: 1
status: public
title: Epilepsy-induced motility of differentiated neurons
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 24
year: '2014'
...
---
_id: '2176'
abstract:
- lang: eng
text: Electron microscopy (EM) allows for the simultaneous visualization of all
tissue components at high resolution. However, the extent to which conventional
aldehyde fixation and ethanol dehydration of the tissue alter the fine structure
of cells and organelles, thereby preventing detection of subtle structural changes
induced by an experiment, has remained an issue. Attempts have been made to rapidly
freeze tissue to preserve native ultrastructure. Shock-freezing of living tissue
under high pressure (high-pressure freezing, HPF) followed by cryosubstitution
of the tissue water avoids aldehyde fixation and dehydration in ethanol; the tissue
water is immobilized in â ̂1/450 ms, and a close-to-native fine structure of cells,
organelles and molecules is preserved. Here we describe a protocol for HPF that
is useful to monitor ultrastructural changes associated with functional changes
at synapses in the brain but can be applied to many other tissues as well. The
procedure requires a high-pressure freezer and takes a minimum of 7 d but can
be paused at several points.
author:
- first_name: Daniel
full_name: Studer, Daniel
last_name: Studer
- first_name: Shanting
full_name: Zhao, Shanting
last_name: Zhao
- first_name: Xuejun
full_name: Chai, Xuejun
last_name: Chai
- first_name: Peter M
full_name: Jonas, Peter M
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
- first_name: Werner
full_name: Graber, Werner
last_name: Graber
- first_name: Sigrun
full_name: Nestel, Sigrun
last_name: Nestel
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
citation:
ama: Studer D, Zhao S, Chai X, et al. Capture of activity-induced ultrastructural
changes at synapses by high-pressure freezing of brain tissue. Nature Protocols.
2014;9(6):1480-1495. doi:10.1038/nprot.2014.099
apa: Studer, D., Zhao, S., Chai, X., Jonas, P. M., Graber, W., Nestel, S., &
Frotscher, M. (2014). Capture of activity-induced ultrastructural changes at synapses
by high-pressure freezing of brain tissue. Nature Protocols. Nature Publishing
Group. https://doi.org/10.1038/nprot.2014.099
chicago: Studer, Daniel, Shanting Zhao, Xuejun Chai, Peter M Jonas, Werner Graber,
Sigrun Nestel, and Michael Frotscher. “Capture of Activity-Induced Ultrastructural
Changes at Synapses by High-Pressure Freezing of Brain Tissue.” Nature Protocols.
Nature Publishing Group, 2014. https://doi.org/10.1038/nprot.2014.099.
ieee: D. Studer et al., “Capture of activity-induced ultrastructural changes
at synapses by high-pressure freezing of brain tissue,” Nature Protocols,
vol. 9, no. 6. Nature Publishing Group, pp. 1480–1495, 2014.
ista: Studer D, Zhao S, Chai X, Jonas PM, Graber W, Nestel S, Frotscher M. 2014.
Capture of activity-induced ultrastructural changes at synapses by high-pressure
freezing of brain tissue. Nature Protocols. 9(6), 1480–1495.
mla: Studer, Daniel, et al. “Capture of Activity-Induced Ultrastructural Changes
at Synapses by High-Pressure Freezing of Brain Tissue.” Nature Protocols,
vol. 9, no. 6, Nature Publishing Group, 2014, pp. 1480–95, doi:10.1038/nprot.2014.099.
short: D. Studer, S. Zhao, X. Chai, P.M. Jonas, W. Graber, S. Nestel, M. Frotscher,
Nature Protocols 9 (2014) 1480–1495.
date_created: 2018-12-11T11:56:09Z
date_published: 2014-05-29T00:00:00Z
date_updated: 2021-01-12T06:55:47Z
day: '29'
department:
- _id: PeJo
doi: 10.1038/nprot.2014.099
intvolume: ' 9'
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 1480 - 1495
project:
- _id: 25BDE9A4-B435-11E9-9278-68D0E5697425
grant_number: SFB-TR3-TP10B
name: Glutamaterge synaptische Übertragung und Plastizität in hippocampalen Mikroschaltkreisen
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '4807'
quality_controlled: '1'
scopus_import: 1
status: public
title: Capture of activity-induced ultrastructural changes at synapses by high-pressure
freezing of brain tissue
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2014'
...